CN113303324A - Fully-closed quick-freezing vitrification cryopreservation tube - Google Patents
Fully-closed quick-freezing vitrification cryopreservation tube Download PDFInfo
- Publication number
- CN113303324A CN113303324A CN202110473624.5A CN202110473624A CN113303324A CN 113303324 A CN113303324 A CN 113303324A CN 202110473624 A CN202110473624 A CN 202110473624A CN 113303324 A CN113303324 A CN 113303324A
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- Prior art keywords
- straw
- metal balance
- fully
- cryopreservation tube
- bar
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- 238000005138 cryopreservation Methods 0.000 title claims abstract description 31
- 238000004017 vitrification Methods 0.000 title claims abstract description 25
- 238000007710 freezing Methods 0.000 title abstract description 20
- 239000010902 straw Substances 0.000 claims abstract description 46
- 239000002184 metal Substances 0.000 claims abstract description 44
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000007789 sealing Methods 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 16
- 239000012472 biological sample Substances 0.000 claims abstract description 14
- 229940099259 vaseline Drugs 0.000 claims description 8
- 229920001634 Copolyester Polymers 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 7
- 239000013078 crystal Substances 0.000 abstract description 6
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 230000005540 biological transmission Effects 0.000 abstract description 3
- 230000005611 electricity Effects 0.000 abstract description 3
- 230000003068 static effect Effects 0.000 abstract description 3
- 238000005728 strengthening Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 description 14
- 210000001161 mammalian embryo Anatomy 0.000 description 7
- 239000010408 film Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 229920003023 plastic Polymers 0.000 description 4
- 239000007789 gas Substances 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 239000004831 Hot glue Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mechanical Engineering (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A fully-closed quick-freezing vitrified cryopreservation tube comprises a half-straw core bar, a tail bar and an outer sleeve, wherein the outer sleeve comprises a metal balance bar, a plug and a transparent conduit, the metal balance bar protrudes out of the plug towards one end of the half-straw core bar, and the other end of the metal balance bar is used for contacting liquid nitrogen; the half-straw core rod is connected with a hard handle and a half-straw blade in sequence towards one end of the outer sleeve, the hard handle is connected with the transparent guide pipe in a sealing mode, and the half-straw blade is arranged right opposite to the metal balance rod and is processed by adopting plasma equipment. The invention forms a rapid transmission channel through the metal balance bar to transmit the low-temperature energy of liquid nitrogen from the outside to the inside of the outer sleeve, thereby ensuring that the vitrification process of the frozen biological sample is smoothly completed, effectively avoiding the formation of ice crystals, strengthening the sealing property between the metal balance bar and the transparent guide pipe through the sealing plug, and removing static electricity through the half straw blades to increase the hydrophilicity, and ensuring that the biological sample to be frozen is firmly attached to the front ends of the half straw blades.
Description
Technical Field
The invention relates to the technical field of embryo storage devices, in particular to the field of fully-closed quick-freezing vitrification cryopreservation tubes.
Background
The human in vitro fertilization embryo transplantation technology widely uses slow freezing and fast thawing methods to carry out cryopreservation of human eggs and embryos, but the method is time-consuming, needs an expensive programmed freezer and has poor freezing effect on the eggs. The vitrification freezing method developed in recent years has an ultra-fast freezing rate due to the adoption of the cryoprotectant with higher concentration, so that the freezing is simpler, faster and more efficient.
At present, most of cryopreservation tubes adopted by human in vitro fertilization embryo transplantation are domestic/imported open vitrification cryopreservation tubes and closed vitrification cryopreservation tubes with two ends being subjected to hot melt adhesive sealing. The open type vitrification freezing storage tube is composed of a thin film (half wheat tube) belt, a hard handle and an outer sleeve, a horn mouth is arranged at the joint of the outer sleeve and the hard handle, and a balance rod is arranged at the tail end of the outer sleeve. The sealed vitrification freezing tube is produced by setting the hard handle part with half straw on the original open vitrification freezing tube, sleeving one outer casing with wall thickness of half millimeter on the hard handle part and hot melting the two ends of the outer casing to seal the glue port to reach the sealed state.
Among the above-mentioned open vitrification cryopreservation tube and closed vitrification cryopreservation tube, the open vitrification cryopreservation tube has the advantages that the liquid nitrogen is directly put into the tube after the embryo is loaded, so that the embryo can quickly reach the freezing temperature, and the ice crystal formation is avoided. In the sealed vitrification freezing storage tube, because the freezing storage liquid on the embryo and the liquid nitrogen are separated by two layers of medium media such as the wall thickness of the rubber tube, gas in the tube and the like, the forming speed of crystal lattices of the freezing storage liquid can be influenced in the vitrification freezing storage process of the embryo, and the outer diameter becomes thicker, so that the short space resources of the storage container are wasted.
Disclosure of Invention
The invention aims to provide a fully-closed quick-freezing vitrification cryopreservation tube which is not directly contacted with liquid nitrogen and is convenient to store.
A fully-closed quick-freezing vitrification cryopreservation tube comprises a half-wheat tube core bar, a tail bar arranged at one end of the half-wheat tube core bar, and an outer sleeve sleeved on the other end of the half-wheat tube core bar and hermetically connected with the half-wheat tube core bar, wherein the outer sleeve comprises a metal balance bar, a plug sleeved on the metal balance bar, and a transparent conduit sleeved on the plug and hermetically connected with the metal balance bar, the metal balance bar protrudes out of the plug towards one end of the half-wheat tube core bar, and the other end of the metal balance bar is used for contacting with liquid nitrogen;
the half-straw core rod is provided with one end facing the outer sleeve pipe, which is sequentially connected with a hard handle and a half-straw blade, the hard handle is hermetically connected with the transparent guide pipe, and the half-straw blade is arranged right opposite to the metal balance bar and is treated by adopting plasma equipment, so that the cryopreserved biological sample is attached to the front end of the half-straw blade.
Compared with the prior art, the rapid transmission channel is formed by the metal balance rod to transmit the low-temperature energy of the liquid nitrogen from the outside to the inside of the outer sleeve, so that the vitrification process of the cryopreserved biological sample is ensured to be smoothly completed, the formation of ice crystals is effectively avoided, the sealing property between the metal balance rod and the transparent guide pipe is enhanced through the sealing plug, the static electricity removal treatment is carried out through the half-straw blade to increase the hydrophilicity, and the biological sample to be cryopreserved can be ensured to be firmly attached to the front end of the half-straw blade.
Furthermore, one end of the hard handle is of a taper structure, and the taper structure is connected with the half straw blade;
be equipped with the recess on the hard handle, be equipped with vaseline film sealing ring in the recess, hard handle pass through vaseline film sealing ring with transparent pipe sealing connection.
Further, the half straw blade is of a transparent spoon-shaped structure.
Further, the transparent conduit is made of copolyester.
Furthermore, one end of the metal balance rod, which faces the half straw core rod, is of a ball head structure, and the outer diameter of the other end of the metal balance rod is 2.5 mm.
Further, the metal balance bar is made of medical stainless steel materials.
Further, the density of the metal balance bar is greater than the density of the transparent conduit.
Furthermore, the section of the hard handle is square, and the side length is 2.5 mm.
Furthermore, a product mark is arranged on one surface of the hard handle, and the product mark is arranged on the same side of the concave surface of the half straw blade.
Furthermore, a patient color identification code is arranged on the tail rod.
Drawings
FIG. 1 is a schematic structural diagram of a fully-enclosed quick-frozen vitrified cryopreservation tube in one embodiment of the invention;
fig. 2 is an exploded view of the fully enclosed quick frozen vitrified cryopreservation tube of fig. 1.
Description of the main element symbols:
| |
Transparent conduit 11 | |||
| Metal balance bar 12 | Closure |
|||
| Half |
|
|||
| | Tail rod | 3 |
The following detailed description will further illustrate the invention in conjunction with the above-described figures.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Several embodiments of the invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1 and 2, a fully-enclosed quick-frozen vitrified cryopreservation tube provided in a first embodiment of the invention includes a half straw core rod 2, a tail rod 3 disposed at one end of the half straw core rod 2, and an outer sleeve 1 sleeved on the other end of the half straw core rod 2 and hermetically connected thereto, wherein the outer sleeve 1 includes a metal balance rod 12, a plug 13 sleeved on the metal balance rod 12, and a transparent conduit 11 sleeved on the plug 13 and hermetically connected to the metal balance rod 12, the metal balance rod 12 protrudes the plug 13 towards one end of the half straw core rod 2, and the other end is used for contacting with liquid nitrogen;
the half-straw core rod 2 is provided with a hard handle 2b and a half-straw blade 2a which are sequentially connected towards one end of the outer sleeve 1, the hard handle 2b is hermetically connected with the transparent conduit 11, and the half-straw blade 2a is arranged right opposite to the metal balance bar 12 and is treated by adopting plasma equipment so that the cryopreserved biological sample is attached to the front end of the half-straw blade 2 a.
It should be noted that, in the present invention, in order to distinguish the activity level of the biological sample, each set of product needs a color mark of five colors, and the color mark is conveniently arranged on the plug 13, and the sealing performance between the metal balance bar 12 and the transparent conduit 11 can be enhanced by the plug 13. A rapid transmission channel is formed by the metal balance bar 12 to transmit the low-temperature energy of the liquid nitrogen from the outside to the inside of the outer sleeve 1, thereby ensuring that the vitrification process of the frozen biological sample is smoothly finished and effectively avoiding the formation of ice crystals. In addition, the static electricity removing treatment is performed through the half straw blades 2a to increase the hydrophilicity thereof, so that it can be ensured that the biological sample to be cryopreserved is firmly attached to the front ends of the half straw blades 2 a.
Referring to fig. 1 and 2, one end of the hard handle 2b is a taper structure, and the taper structure is connected with the half straw blade 2 a;
be equipped with the recess on the hard handle 2b, be equipped with vaseline film sealing ring in the recess, hard handle 2b pass through vaseline film sealing ring with transparent pipe 11 sealing connection.
It should be noted that, in this embodiment, a vaseline film is injected into the annular groove at the joint of the transparent conduit 11 and the hard handle 2b and the vaseline film is in taper fit with the annular groove, so as to achieve a high sealing function, when the gas in the sealed transparent conduit 11 is contracted when being cooled, the wall of the transparent conduit 11 is also tightened, and the inner wall is tightly attached to the hard handle 2b, so as to reinforce the sealing property, achieve a real sealing effect (no gas leakage or liquid leakage), avoid the contact between liquid nitrogen and a biological sample, and reduce the risk of infection of biological cells.
In a preferred embodiment of the present invention, the half straw blade 2a is a transparent spoon-like structure to facilitate the storage of the cryopreserved biological sample.
In another preferred embodiment of the present invention, the transparent conduit 11 is made of copolyester. Specifically, the copolyester is a mixed color master polymer structure and can be tightly sewn and molded with the metal balance bar 12.
Referring to fig. 1 and 2, in a preferred embodiment of the present invention, one end of the metal balance bar 12 facing the half straw core bar 2 is of a bulb structure, and the other end has an outer diameter of 2.5 mm. Specifically, the ball head structure is a naked metal ball head without any electroplated layer, the outer end immersed in liquid nitrogen is cylindrical, and the low-temperature energy of the liquid nitrogen is radiated to an adjacent biological sample at the ball head structure through a conduction channel of the metal balance rod 12 by utilizing the high-speed heat conduction property of metal, so that the vitrification cryopreservation effect is achieved, and the formation of crystal lattices is avoided.
In another preferred embodiment of the present invention, the metal balance bar 12 is made of medical grade stainless steel material.
In a preferred embodiment of the present invention, the density of the metal balance bar 12 is greater than that of the transparent conduit 11, so as to ensure that the buoyancy effect of the transparent conduit 11 in liquid nitrogen can be counteracted by impact during freezing.
In a preferred embodiment of the invention, the hard handle 2b has a square cross section with a side length of 2.5 mm. Thereby being consistent with the external diameter of the bottom of the metal balance bar 12, ensuring the tightness without increasing the peripheral dimension and effectively controlling the space of the refrigeration storage.
It should be noted that, because the smallest unit container for loading the vitrification carrier in the liquid nitrogen container is a cylindrical plastic box, the external shape and the cross-sectional dimension of the vitrification cryopreservation tube directly affect the capacity of the liquid nitrogen container for storing the biological sample. For example, the smallest unit container on the market is a cylindrical plastic box with a diameter of 10mm and a minimum diameter size of the cross section of the vitrified cryopreservation tube of 3mm, and the cylindrical plastic box can store 6 vitrified cryopreservation tubes. In the invention, the minimum diameter of the section of the vitrification cryopreservation tube is 2.5mm, and the cylindrical plastic box can store 7 vitrification cryopreservation tubes. In contrast, the present invention has the advantage of saving and utilizing the limited freezer resources to the maximum extent, since the largest storage carrier can store the largest number of samples per unit area.
Furthermore, a product mark is arranged on one surface of the hard handle 2b, and the product mark is arranged on the same side of the concave surface of the half straw blade 2 a. For example, the product identifier may be a company LOGO.
Further, a patient color identification code, that is, basic information of the patient, is provided on the tail rod 3, so that identification is convenient, and it can be understood that the patient color identification code may be a carrier capable of storing information, such as characters, figures, two-dimensional codes and the like related to storage.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The utility model provides a totally enclosed quick-freeze vitrification cryopreserved pipe, includes half straw core bar, locates the tailpiece of half straw core bar one end, and the cover is located on half straw core bar other end and sealing connection's outer tube, its characterized in that: the outer sleeve comprises a metal balance rod, a plug and a transparent conduit, wherein the plug is sleeved on the metal balance rod, the transparent conduit is sleeved on the plug and is in sealing connection with the metal balance rod, one end of the metal balance rod, which faces to the half wheat straw core rod, protrudes out of the plug, and the other end of the metal balance rod is used for being in contact with liquid nitrogen;
the half-straw core rod is provided with one end facing the outer sleeve pipe, which is sequentially connected with a hard handle and a half-straw blade, the hard handle is hermetically connected with the transparent guide pipe, and the half-straw blade is arranged right opposite to the metal balance bar and is treated by adopting plasma equipment, so that the cryopreserved biological sample is attached to the front end of the half-straw blade.
2. The fully-closed quick-frozen vitrified cryopreservation tube of claim 1, which is characterized in that: one end of the hard handle is a taper structure, and the taper structure is connected with the half straw blade;
be equipped with the recess on the hard handle, be equipped with vaseline film sealing ring in the recess, hard handle pass through vaseline film sealing ring with transparent pipe sealing connection.
3. The fully-closed quick-frozen vitrified cryopreservation tube of claim 1, which is characterized in that: the half straw blade is of a transparent spoon-shaped structure.
4. The fully-closed quick-frozen vitrified cryopreservation tube of claim 2, which is characterized in that: the transparent conduit is made of copolyester.
5. The fully-closed quick-frozen vitrified cryopreservation tube of claim 1, which is characterized in that: one end of the metal balance rod, which faces the half straw core rod, is of a ball head structure, and the outer diameter of the other end of the metal balance rod is 2.5 mm.
6. The fully-closed quick-frozen vitrified cryopreservation tube of claim 1, which is characterized in that: the metal balance bar is made of medical stainless steel materials.
7. The fully-closed quick-frozen vitrified cryopreservation tube of claim 1, which is characterized in that: the density of the metal balance bar is greater than that of the transparent conduit.
8. The fully-closed quick-frozen vitrified cryopreservation tube of claim 1, which is characterized in that: the section of the hard handle is square, and the side length is 2.5 mm.
9. The fully-closed quick-frozen vitrified cryopreservation tube of claim 1, which is characterized in that: and a product mark is arranged on one surface of the hard handle and is positioned at the same side as the concave surface of the half straw blade.
10. The fully-closed quick-frozen vitrified cryopreservation tube of claim 1, which is characterized in that: and the tail rod is provided with a patient color identification code.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110473624.5A CN113303324A (en) | 2021-04-29 | 2021-04-29 | Fully-closed quick-freezing vitrification cryopreservation tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110473624.5A CN113303324A (en) | 2021-04-29 | 2021-04-29 | Fully-closed quick-freezing vitrification cryopreservation tube |
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| CN113303324A true CN113303324A (en) | 2021-08-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110473624.5A Pending CN113303324A (en) | 2021-04-29 | 2021-04-29 | Fully-closed quick-freezing vitrification cryopreservation tube |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107836446A (en) * | 2017-10-31 | 2018-03-27 | 江西易通医疗器械有限公司 | Cryopreservation tube |
| CN108925549A (en) * | 2018-08-10 | 2018-12-04 | 中国科学院深圳先进技术研究院 | A kind of cell cryopreservation carries bar and its preparation method and application |
| CN208509955U (en) * | 2018-05-31 | 2019-02-19 | 南京鼓楼医院 | A kind of miniature save set frozen for human testicle and epididymal sperm |
| CN215957995U (en) * | 2021-04-29 | 2022-03-08 | 江西康雅医疗用品有限公司 | Fully-closed quick-freezing vitrification cryopreservation tube |
-
2021
- 2021-04-29 CN CN202110473624.5A patent/CN113303324A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107836446A (en) * | 2017-10-31 | 2018-03-27 | 江西易通医疗器械有限公司 | Cryopreservation tube |
| CN208509955U (en) * | 2018-05-31 | 2019-02-19 | 南京鼓楼医院 | A kind of miniature save set frozen for human testicle and epididymal sperm |
| CN108925549A (en) * | 2018-08-10 | 2018-12-04 | 中国科学院深圳先进技术研究院 | A kind of cell cryopreservation carries bar and its preparation method and application |
| CN215957995U (en) * | 2021-04-29 | 2022-03-08 | 江西康雅医疗用品有限公司 | Fully-closed quick-freezing vitrification cryopreservation tube |
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