CN113272649B - 用于分析病毒衣壳蛋白组成的方法 - Google Patents

用于分析病毒衣壳蛋白组成的方法 Download PDF

Info

Publication number
CN113272649B
CN113272649B CN201980069087.9A CN201980069087A CN113272649B CN 113272649 B CN113272649 B CN 113272649B CN 201980069087 A CN201980069087 A CN 201980069087A CN 113272649 B CN113272649 B CN 113272649B
Authority
CN
China
Prior art keywords
capsid
virus
viral
aav
particle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201980069087.9A
Other languages
English (en)
Chinese (zh)
Other versions
CN113272649A (zh
Inventor
王顺海
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Regeneron Pharmaceuticals Inc
Original Assignee
Regeneron Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Priority to CN202410186592.4A priority Critical patent/CN117969861A/zh
Publication of CN113272649A publication Critical patent/CN113272649A/zh
Application granted granted Critical
Publication of CN113272649B publication Critical patent/CN113272649B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/20Partition-, reverse-phase or hydrophobic interaction chromatography
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102Alpha-glucosidase (3.2.1.20)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/62Detectors specially adapted therefor
    • G01N30/72Mass spectrometers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/62Detectors specially adapted therefor
    • G01N30/72Mass spectrometers
    • G01N30/7233Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/14011Baculoviridae
    • C12N2710/14111Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141Use of virus, viral particle or viral elements as a vector
    • C12N2750/14142Use of virus, viral particle or viral elements as a vector virus or viral particle as vehicle, e.g. encapsulating small organic molecule
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151Methods of production or purification of viral material
    • C12N2750/14152Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N2030/022Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027Liquid chromatography
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/04Preparation or injection of sample to be analysed
    • G01N30/06Preparation
    • G01N2030/062Preparation extracting sample from raw material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01DNA viruses
    • G01N2333/015Parvoviridae, e.g. feline panleukopenia virus, human Parvovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980069087.9A 2018-10-25 2019-10-24 用于分析病毒衣壳蛋白组成的方法 Active CN113272649B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202410186592.4A CN117969861A (zh) 2018-10-25 2019-10-24 用于分析病毒衣壳蛋白组成的方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862750583P 2018-10-25 2018-10-25
US62/750,583 2018-10-25
PCT/US2019/057936 WO2020086893A1 (en) 2018-10-25 2019-10-24 Methods for analysis of viral capsid protein composition

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202410186592.4A Division CN117969861A (zh) 2018-10-25 2019-10-24 用于分析病毒衣壳蛋白组成的方法

Publications (2)

Publication Number Publication Date
CN113272649A CN113272649A (zh) 2021-08-17
CN113272649B true CN113272649B (zh) 2024-03-12

Family

ID=68696510

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201980069087.9A Active CN113272649B (zh) 2018-10-25 2019-10-24 用于分析病毒衣壳蛋白组成的方法
CN202410186592.4A Pending CN117969861A (zh) 2018-10-25 2019-10-24 用于分析病毒衣壳蛋白组成的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202410186592.4A Pending CN117969861A (zh) 2018-10-25 2019-10-24 用于分析病毒衣壳蛋白组成的方法

Country Status (17)

Country Link
US (4) US11345929B2 (https=)
EP (2) EP3870976B1 (https=)
JP (2) JP7439077B2 (https=)
KR (1) KR102839007B1 (https=)
CN (2) CN113272649B (https=)
AU (2) AU2019366983B2 (https=)
BR (1) BR112021007550A2 (https=)
CA (1) CA3115832A1 (https=)
EA (1) EA202191137A1 (https=)
ES (1) ES2968865T3 (https=)
IL (1) IL282411B2 (https=)
MX (1) MX2021004533A (https=)
MY (1) MY205165A (https=)
PL (1) PL3870976T3 (https=)
SG (1) SG11202103463RA (https=)
WO (1) WO2020086893A1 (https=)
ZA (2) ZA202102343B (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12017255B2 (en) 2015-07-16 2024-06-25 Sortera Technologies, Inc. Sorting based on chemical composition
US12280403B2 (en) 2015-07-16 2025-04-22 Sortera Technologies, Inc. Sorting based on chemical composition
US12290842B2 (en) 2015-07-16 2025-05-06 Sortera Technologies, Inc. Sorting of dark colored and black plastics
EP4076537A4 (en) * 2019-12-20 2024-02-28 University of Maryland, Baltimore NON-INVASIVE QUANTIFICATION OF FULL CAPSIDS VS. VACUUMS USING PROTON NMR OF WATER
US20240052322A1 (en) * 2020-12-15 2024-02-15 Pfizer Inc. HILIC UPLC-MS Method For Separating and Analyzing Intact Adeno-Associated Virus Capsid Proteins
TWI829131B (zh) * 2021-09-28 2024-01-11 美商索特拉科技公司 用於淘選材料之方法和系統以及儲存在電腦可讀儲存媒體上的電腦程式產品
EP4514984A4 (en) * 2022-04-25 2026-04-22 Beijing Hanmoluojie Tech Co Ltd 3D MOLECULAR SURFACE FEATURES MAPPING OF AAV CAPSIDS
WO2024249438A1 (en) * 2023-05-26 2024-12-05 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Copiparvovirus genetic vector platform
WO2025035009A1 (en) * 2023-08-08 2025-02-13 Brammer Bio, Llc Detection of variability in viruses

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101910841A (zh) * 2007-10-29 2010-12-08 佐治亚大学研究基金会 用于定量糖组学的体内同位素标记方法
CN107228912A (zh) * 2017-05-26 2017-10-03 河南省兽药饲料监察所 一种动物性食品中79种抗菌药物的筛查方法
WO2018035059A1 (en) * 2016-08-15 2018-02-22 Genzyme Corporation Methods for detecting aav

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0931158A1 (en) 1996-09-06 1999-07-28 The Trustees Of The University Of Pennsylvania An inducible method for production of recombinant adeno-associated viruses utilizing t7 polymerase
US7754421B2 (en) * 2005-11-18 2010-07-13 National Research Council Of Canada Detection of intact recombinant viruses
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
WO2012058619A1 (en) 2010-10-29 2012-05-03 Cohesive Technologies Inc. Lc-ms configuration for purification and detection of analytes having a broad range of hydrophobicities
CN103827134A (zh) * 2011-07-20 2014-05-28 泽普泰恩股份有限公司 多肽分离方法
US8946395B1 (en) * 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
EP2975401B1 (en) 2014-07-18 2019-12-25 Hexal AG Improved method of mapping glycans of glycoproteins in serum samples
CA3012195A1 (en) * 2016-02-03 2017-08-10 The Trustees Of The University Of Pennsylvania Gene therapy for treating mucopolysaccharidosis type i
JPWO2018003559A1 (ja) 2016-07-01 2019-02-07 日立オートモティブシステムズ株式会社 燃料噴射弁

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101910841A (zh) * 2007-10-29 2010-12-08 佐治亚大学研究基金会 用于定量糖组学的体内同位素标记方法
WO2018035059A1 (en) * 2016-08-15 2018-02-22 Genzyme Corporation Methods for detecting aav
CN107228912A (zh) * 2017-05-26 2017-10-03 河南省兽药饲料监察所 一种动物性食品中79种抗菌药物的筛查方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
液相色谱-质谱联用方法用于严重急性呼吸系统综合症冠状病毒结构蛋白质的研究;张养军,王京兰,蔡耘,应万涛,郝运伟,李蕾,李晓海,戴舒佳,代景泉,贺福初,钱小红,王杰,魏开华;分析化学(第04期);全文 *

Also Published As

Publication number Publication date
EP4306961A3 (en) 2024-04-17
JP7577719B2 (ja) 2024-11-05
US20200299728A1 (en) 2020-09-24
CN117969861A (zh) 2024-05-03
CN113272649A (zh) 2021-08-17
IL282411B1 (en) 2025-05-01
AU2019366983A1 (en) 2021-05-13
JP2022505687A (ja) 2022-01-14
PL3870976T3 (pl) 2024-04-08
JP2023021996A (ja) 2023-02-14
AU2019366983B2 (en) 2025-10-16
SG11202103463RA (en) 2021-05-28
MX2021004533A (es) 2021-08-24
MY205165A (en) 2024-10-04
US11981913B2 (en) 2024-05-14
EA202191137A1 (ru) 2021-10-06
CA3115832A1 (en) 2020-04-30
EP3870976B1 (en) 2023-11-29
BR112021007550A2 (pt) 2021-07-27
ZA202203800B (en) 2024-08-28
ZA202102343B (en) 2024-07-31
EP3870976A1 (en) 2021-09-01
AU2026200075A1 (en) 2026-01-22
KR102839007B1 (ko) 2025-07-29
US20240102046A9 (en) 2024-03-28
WO2020086893A1 (en) 2020-04-30
US11345929B2 (en) 2022-05-31
IL282411B2 (en) 2025-09-01
JP7439077B2 (ja) 2024-02-27
US20220275398A1 (en) 2022-09-01
KR20210082183A (ko) 2021-07-02
EP4306961A2 (en) 2024-01-17
US20200131533A1 (en) 2020-04-30
ES2968865T3 (es) 2024-05-14
US20240084328A1 (en) 2024-03-14
IL282411A (en) 2021-06-30
US12480139B2 (en) 2025-11-25
US11359213B2 (en) 2022-06-14

Similar Documents

Publication Publication Date Title
CN113272649B (zh) 用于分析病毒衣壳蛋白组成的方法
US20230016717A1 (en) Methods for Viral Particle Characterization Using Two-Dimensional Liquid Chromatography-Mass Spectrometry
US20230010418A1 (en) Online Native Mass Spectrometry Methods for Assaying Viral Particles
US20240052322A1 (en) HILIC UPLC-MS Method For Separating and Analyzing Intact Adeno-Associated Virus Capsid Proteins
HK40105747A (en) Methods for analysis of viral capsid protein composition
HK40055573A (en) Methods for analysis of viral capsid protein composition
HK40055573B (en) Methods for analysis of viral capsid protein composition
EA053124B1 (ru) Способы анализа композиции вирусного капсидного белка
EA045266B1 (ru) Способы анализа композиции вирусного капсидного белка
CN117795334A (zh) 使用二维液相色谱-质谱进行病毒颗粒表征的方法
CN117859060A (zh) 用于测定病毒颗粒的在线非变性质谱方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant