CN113229277B - Low-temperature spraying cationic disinfectant - Google Patents
Low-temperature spraying cationic disinfectant Download PDFInfo
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- CN113229277B CN113229277B CN202110111603.9A CN202110111603A CN113229277B CN 113229277 B CN113229277 B CN 113229277B CN 202110111603 A CN202110111603 A CN 202110111603A CN 113229277 B CN113229277 B CN 113229277B
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- disinfectant
- calcium chloride
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to a low-temperature spraying cationic disinfectant, which comprises a cationic disinfectant and calcium chloride, wherein the mass percentage of the calcium chloride in the prepared disinfectant is 1-30%. According to the invention, the combination of calcium chloride and the cationic disinfectant has the advantages that on one hand, the freezing point of the calcium chloride solution is low, so that the disinfectant can be kept in a liquid state at a low temperature, and the spray disinfection can be normally carried out; on the other hand, the solutions of the cationic disinfectant and the calcium chloride are alkaline, so that the cationic disinfectant and the calcium chloride are mixed with each other, the disinfection effect can be effectively improved, and the cationic disinfectant and the calcium chloride have a good disinfection effect on cells in a low-temperature dormant state.
Description
Technical Field
The invention relates to a low-temperature spraying cationic disinfectant, and belongs to the field of disinfectants.
Background
The spray disinfection is a method for disinfecting by uniformly spraying a chemical disinfectant on the air or the surface of an object by using a sprayer, can effectively kill pathogenic microorganisms in the culture and transportation process, greatly reduces the content of the pathogenic microorganisms, controls the spread of diseases and reduces the risk of diseases in culture. The disinfecting effect of disinfectants almost all disinfectants have a significantly reduced or even ineffective disinfecting effect with decreasing ambient temperature. Especially, most of northern areas have the temperature below zero, and some areas can have the temperature as low as-36 ℃. In these places, if spray disinfection is used, the disinfectant freezes without adding an antifreeze, and thus there is no disinfection effect. In addition, if the antifreeze is added improperly, the disinfection effect is reduced. In addition, under the condition of low temperature, the bacteria can enter a dormant state by self, so that the disinfection is difficult and the disinfection effect is poor.
Disclosure of Invention
The invention relates to a low-temperature spraying cationic disinfectant, which solves the problem that the disinfectant has poor disinfection effect and even fails under the low-temperature condition.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
the low-temperature spraying cationic disinfectant comprises a cationic disinfectant and calcium chloride, wherein the mass percentage of the calcium chloride in the prepared disinfectant is 1-30%. The concentration of the actual calcium chloride can be determined according to the environment of the spray disinfection work and the freezing point of the calcium chloride solution, and the mass percentage of the calcium chloride is set to be 1% -30%, so that the working temperature of the disinfectant is 0-55 ℃, and the disinfectant can be effectively used in most areas in the north. The dosage of the cationic disinfectant in the disinfectant and the disinfection environment are determined,
preferably: the cationic disinfectant is quaternary ammonium salt disinfectant.
Preferably: the quaternary ammonium salt disinfectant is benzalkonium bromide, benzalkonium chloride and decamethyl bromide.
Preferably: the low-temperature spraying cationic disinfectant also comprises a chelating agent, and the mass percentage of the chelating agent in the prepared disinfectant is 0.2% -2%.
Preferably: the chelating agent is disodium EDTA.
The invention has the beneficial effects that:
according to the invention, the combination of the cationic disinfectant and calcium chloride is adopted, firstly, cationic hydrophilic groups in the cationic disinfectant adsorb negatively charged bacteria, and the negatively charged bacteria are gathered on cell walls to generate a chamber resistance effect, so that the bacteria are inhibited in growth and die; meanwhile, the hydrophobic alkyl can also act with the hydrophilic group of bacteria to change the permeability of the membrane, so that lysis is generated, the cell structure is damaged, and the cell is dissolved and killed; the low-temperature calcium chloride can enable the cells to enter a damaged state, the calcium chloride can enable exogenous calcium ions to be adsorbed on the surfaces of the competent cells, and after the cells are revived, the external disinfectant can enter the inside of the cells more easily, so that the aim of disinfection is fulfilled. According to the combination of the calcium chloride and the cationic disinfectant, on one hand, the freezing point of the calcium chloride solution is low, so that the disinfectant can be kept in a liquid state at low temperature, and the spray disinfection can be normally carried out; on the other hand, the solutions of the cationic disinfectant and the calcium chloride are alkaline, so that the cationic disinfectant and the calcium chloride are mixed with each other, the disinfection effect can be effectively improved, and the cationic disinfectant and the calcium chloride have a good disinfection effect on cells in a low-temperature dormant state.
Compared with a liquid antifreezing agent, the calcium chloride is convenient to transport, has small influence on the environment, and is more favorable for popularization and use of spray disinfection in winter.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A low-temperature spray cationic disinfectant comprises trimercapto-ammonia chloride and calcium chloride, wherein the mass percentage of the calcium chloride in the prepared disinfectant is 18%.
The content of the benzalkonium chloride in the disinfectant is diluted by tap water or well water according to a required proportion.
Experiment of Disinfection Effect
Preparation of bacterial suspension: the suspension of colibacillus, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and bacillus subtilis used in the test is cultured according to a conventional method, then the suspension is cultured for 48 hours on a slope surface in a growth logarithmic phase and is scribed, then the suspension is stored for 24 hours at the temperature of minus 15 ℃, then the strain is taken from the slope surface, prepared by evenly sterilized normal saline and stored for standby at the temperature of minus 4 ℃.
Killing test of different disinfectants on bacteria at low temperature: diluting the bacterial suspension with 10 times of precooled various disinfectant at the temperature of-15-20 ℃, immediately taking out 0.5ml after 2min of action, adding the diluted bacterial suspension into 4.5ml of a neutralizer after sterilization, neutralizing for 10min, then taking 1ml of the diluted bacterial suspension with sterilized normal saline, finally taking l ml of the mixed bacterial suspension, coating the mixed bacterial suspension on a flat plate in an incubator at the temperature of 37 ℃, and counting bacterial colonies on the flat plate after culturing for 48 hours, wherein the specific sterilization rate is shown in table 1.
TABLE 1 Low temperature Disinfection Effect of Trimethotrimercapto/calcium chloride
Note: the benzyl trimethyl ammonium chloride and 38% ethanol solution can make the freezing point of the disinfectant at about-30 ℃.
The effect experiment of the invention is different from the conventional disinfection effect experiment, the bacteria with the measurement is stored for 24 hours at the temperature of 15 ℃ below zero in the low-temperature environment, and the bacteria are subjected to the temperature consistent with the disinfection environment, so that the strains are in the low-temperature dormant state, and the bacteria are closer to the actual environment. The solution of benzalkonium chloride and 18% calcium chloride is liquid at-15-20 deg.C, and can be spray sterilized. As can be seen from the data in Table 1, the trimercapto chloride + 18% calcium chloride has a good disinfection effect on bacteria such as escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, and compared with the combination of the trimercapto chloride and 38% ethanol solution, the sterilization composition can achieve the effect of the trimercapto chloride + 38% ethanol solution in a dilution ratio of 1:1200, and can achieve a good disinfection effect with a small dosage. For two kinds of bacteria, namely candida albicans (mold) and bacillus subtilis, dormant spores and spores can be formed at low temperature, the trimercapto chloride and 18% calcium chloride have good disinfection effect at a dilution ratio of 1:800, and the trimercapto chloride and 38% ethanol solution has poor disinfection effect for the candida albicans (mold) and the bacillus subtilis, and the sterilization rate is 92.13% and 93.28%. Therefore, the combination of the trimeresuryl chloride and the calcium chloride has good disinfection effect on bacteria in low-temperature environment.
Example 2
A low-temperature spraying cationic disinfectant comprises benzalkonium bromide and calcium chloride, wherein the mass percentage of the calcium chloride in the prepared disinfectant is 1%. The disinfectant has good disinfection effect at 0-5 ℃, and the sterilization rates of 0.1 percent of benzalkonium bromide and calcium chloride mixed disinfectant to escherichia coli, staphylococcus aureus and pseudomonas aeruginosa are respectively 100 percent, 100 percent and 99.99 percent at 0-5 ℃; the sterilization rates of Candida albicans and Bacillus subtilis are respectively 99.99% and 99.98%
Example 3
A low-temperature spraying cationic disinfectant comprises ammonium decamethyl bromide and calcium chloride, wherein the mass percentage of the calcium chloride in the prepared disinfectant is 20%. 0.01 percent of mixed disinfectant of decamethylammonium bromide and calcium chloride with the temperature below 20 ℃ below zero has good disinfection effect, and the sterilization rates on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa are respectively 100 percent, 99.99 percent and 99.99 percent; the sterilization rates for Candida albicans and Bacillus subtilis were 99.99% and 99.99%, respectively.
Example 4
The low-temperature spraying cationic disinfectant comprises trimercapto chloride, calcium chloride and disodium EDTA, wherein the mass percent of the calcium chloride in the prepared disinfectant is 18%, and the mass percent of the disodium EDTA in the prepared disinfectant is 2%.
The bactericidal rate of the benzalkonium chloride diluted by 1:1200 to escherichia coli, staphylococcus aureus and pseudomonas aeruginosa is 100%, 99.99% and 100% respectively; the sterilization rates for Candida albicans and Bacillus subtilis were 99.98% and 99.96%, respectively.
The EDTA disodium is an excellent chelating agent, can effectively chelate calcium in the disinfectant, plays a role in stabilizing the disinfectant, can effectively reduce the blockage of a spray head in the spraying process, can also effectively improve the sterilization effect, and particularly has an important role in improving the sterilization effect of Candida albicans and Bacillus subtilis.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (3)
1. A low-temperature spraying cationic disinfectant is characterized in that: the disinfectant consists of a cationic disinfectant and calcium chloride, wherein the calcium chloride accounts for 1-30% of the prepared disinfectant by mass; the cationic disinfectant is a quaternary ammonium salt disinfectant; the quaternary ammonium salt disinfectant is benzalkonium bromide, benzalkonium chloride and decamethyl bromide.
2. A low-temperature spraying cationic disinfectant is characterized in that: the low-temperature spraying cationic disinfectant consists of a cationic disinfectant, calcium chloride and a chelating agent, wherein the mass percent of the calcium chloride in the prepared disinfectant is 1% -30%; the cationic disinfectant is a quaternary ammonium salt disinfectant; the quaternary ammonium salt disinfectant is benzalkonium bromide, benzalkonium chloride and decamethyl bromide, and the chelating agent accounts for 0.2-2 wt% of the prepared disinfectant.
3. A low temperature spray cationic disinfectant as claimed in claim 2, wherein: the chelating agent is disodium EDTA.
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| CN202110111603.9A CN113229277B (en) | 2021-01-27 | 2021-01-27 | Low-temperature spraying cationic disinfectant |
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| CN113229277B true CN113229277B (en) | 2022-08-16 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101233845A (en) * | 2008-02-14 | 2008-08-06 | 中国检验检疫科学研究院检测技术与装备研究所 | Disinfection antifreeze agent and preparation thereof |
| WO2018031067A1 (en) * | 2016-08-11 | 2018-02-15 | Ecolab Usa Inc. | Interaction between antimicrobial quaternary compounds and anionic surfactants |
| CN106472502A (en) * | 2016-09-21 | 2017-03-08 | 西安乐道生物科技有限公司 | A kind of breeding environment antibacterial and preparation method thereof |
| CN106577820A (en) * | 2016-12-23 | 2017-04-26 | 江西宜信堂医疗科技有限公司 | Sanitizer for air sterilization and preparation method thereof |
| CN107810952A (en) * | 2017-11-02 | 2018-03-20 | 成都金川田农机制造有限公司 | A kind of Freezeproof foam disinfectant |
| CN111096322A (en) * | 2019-12-31 | 2020-05-05 | 内蒙古农业大学 | Glutaraldehyde decamethylammonium bromide composite disinfectant suitable for low-temperature use |
| CN112704076B (en) * | 2020-12-30 | 2021-08-24 | 中国疾病预防控制中心环境与健康相关产品安全所 | Low-temperature disinfectant and preparation and application thereof |
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