CN113229147A - Method for cultivating differentiated bud of butterfly orchid seedling by using extreme illumination - Google Patents
Method for cultivating differentiated bud of butterfly orchid seedling by using extreme illumination Download PDFInfo
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- CN113229147A CN113229147A CN202110631791.8A CN202110631791A CN113229147A CN 113229147 A CN113229147 A CN 113229147A CN 202110631791 A CN202110631791 A CN 202110631791A CN 113229147 A CN113229147 A CN 113229147A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a method for cultivating a differentiated bud of a butterfly orchid seedling by using extreme illumination, which comprises the following steps: dividing each day into an illumination section and a black light section, wherein the illumination section is in 18-20h, the black light section is in 4-6h, and the black light section is in the highest outdoor temperature period under natural conditions; the illumination section comprises a first illumination section and a second illumination section, and the illumination directions of the butterfly orchid explants in the proliferation stage in the first illumination section and the second illumination section are symmetrical. The method utilizes extreme light to cultivate the differentiated bud of the butterfly orchid seedling, thereby reducing energy consumption and cultivation cost.
Description
Technical Field
The invention relates to cultivation of phalaenopsis, in particular to a method for cultivating differentiation buds of phalaenopsis seedlings by using extreme light.
Background
The plant tissue culture is also called in vitro culture, and means that a tissue meeting the requirement is separated from a plant body. The tissue culture comprises the steps of explant selection, explant sterilization, explant inoculation, bud proliferation (including adventitious bud induction and adventitious bud induction), root induction, test-tube seedling bottle emergence, transplantation and the like.
Tissue culture has been widely used in the process of butterfly orchid culture, in the bud multiplication stage, explants are multiplied after being inoculated on a culture medium by the continuous formation and growth of lateral buds, and adventitious buds and embryoid bodies are generated on the explants or callus tissues. At present, lateral buds and adventitious buds are most used in tissue culture for rapid propagation of phalaenopsis.
At present, the bud propagation stage needs to give the explant enough strong light (generally 1500-. To this situation, need cool down the bottle seedling room, artifical cooling needs consume very big energy to increase cultivation cost, also do not benefit to the environmental protection simultaneously.
Disclosure of Invention
The invention aims to provide a method for cultivating a differentiated bud of a phalaenopsis seedling by using extreme light, which is used for cultivating the differentiated bud of the phalaenopsis seedling by using the extreme light, so that the energy consumption is reduced, and the cultivation cost is reduced.
In order to achieve the above object, the present invention provides a method for cultivating a differentiated bud of a butterfly orchid seedling by extreme light, comprising: dividing each day into an illumination section and a black light section, wherein the illumination section is in 18-20h, the black light section is in 4-6h, and the black light section is in the highest outdoor temperature period under natural conditions; the illumination section comprises a first illumination section and a second illumination section, and the illumination directions of the butterfly orchid explants in the proliferation stage in the first illumination section and the second illumination section are symmetrical.
Preferably, the time of the first illumination period and the second illumination period is 9-10h independently.
Preferably, in the first illumination section and the second illumination section, the illumination intensity is 1500-.
Preferably, the black light segment is between 10 and 4 points per day.
Preferably, the time of the first illumination section and the time of the second illumination section are both 10h, and the time of the black illumination section is 4 h.
Preferably, the black light segment is 11-3 points per day.
Preferably, the total time of incubation in the method is 20-45 days.
Preferably at 225m3The bottle seedling room is taken as a reference, the total air delivery volume is 1100-1150m3/h。
Preferably at 225m3The bottle seedling room is used as a reference, the number of the air conveying openings is 6-10, and the area of each conveying opening is 0.2-0.4m2。
Preferably, the method further comprises: filtering the air before the air is conveyed to the seedling chamber.
In the technical scheme, the cultivation time of each day is creatively divided into an illumination section and a black light section, wherein the illumination section illuminates the explant to induce the explant to differentiate buds, and the light source is turned off in the black light section to directly convey air into the bottle seedling chamber, so that the bottle seedling chamber is cooled; more importantly, in the invention, the illumination section comprises a first illumination section and a second illumination section, the illumination directions of the butterfly orchid explant in the proliferation stage in the first illumination section and the second illumination section are symmetrical, illumination is performed in a symmetrical illumination mode, the illumination intensity of a single side face of the butterfly orchid explant is ensured, and the first illumination section and the second illumination section are performed separately, so that the temperature in the bottle seedling chamber is mild and is generally 23-28 ℃, and additional cooling is not needed.
Therefore, compared with the prior art that the omni-directional illumination is 11-12h (the omni-directional illumination refers to the integral synchronous illumination of the seedling chamber), the method adopts a half-to-half illumination mode, namely the first illumination section and the second illumination section respectively illuminate two sides of the explant, so that extra cooling is not needed at the illumination moment, and the illumination intensity required by induced differentiation buds is ensured; the existing black light section is generally cooled by an air conditioner, the energy consumption is high, and the mode of conveying air is adopted for cooling in the application, so that the energy consumption is further reduced, and the cost is saved.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a pictorial view of a differentiated shoot obtained in example 1;
FIG. 2 is a pictorial view of the differentiated shoot obtained in comparative example 1;
FIG. 3 is a graph comparing the results of example 1 and comparative example 1.
Detailed Description
The following describes in detail specific embodiments of the present invention. It should be understood that the detailed description and specific examples, while indicating the present invention, are given by way of illustration and explanation only, not limitation.
The invention provides a method for cultivating a differentiated bud of a butterfly orchid seedling by using extreme illumination, which comprises the following steps: dividing each day into an illumination section and a black light section, wherein the illumination section is in 18-20h, the black light section is in 4-6h, and the black light section is in the highest outdoor temperature period under natural conditions; the illumination section comprises a first illumination section and a second illumination section, and the illumination directions of the butterfly orchid explants in the proliferation stage in the first illumination section and the second illumination section are symmetrical.
In the present invention, the respective time of the first illumination period and the second illumination period is not particularly limited, but in order to enable the phalaenopsis explants to induce differentiation of more buds, the time of the first illumination period and the second illumination period is preferably 9-10h independently.
In the present invention, the illumination intensity of the first illumination segment and the second illumination segment is not particularly limited, but in order to enable the phalaenopsis explants to induce the differentiation of more buds, the illumination intensity in the first illumination segment and the second illumination segment is preferably 1500-.
In the present invention, the kind of the light source of the first illumination section and the second illumination section is not particularly limited, but in order to enable the phalaenopsis explants to induce more bud differentiation, preferably, in the first illumination section and the second illumination section, the light source is selected from one of the LED lamps with power of 21-28W.
In the present invention, the black light segment is not specifically limited, and needs to be adaptively adjusted according to regions and seasons, and taking an Anhui region as an example, the black light segment is calculated according to Beijing time, and preferably, the black light segment is between 10 and 4 points per day.
On the basis, in order to further enable the phalaenopsis explant to induce and differentiate more buds, preferably, the time of the first illumination section and the time of the second illumination section are both 10h, and the time of the black illumination section is 4 h.
On the premise that the time of the black light segment is 4h, the black light segment is preferably 11-3 points per day.
In the present invention, the total time of incubation in the method is not particularly limited, but in order to allow the phalaenopsis explants to induce differentiation of more buds, it is preferred that the total time of incubation in the method is 20-45 days.
In the above embodiment, the total delivery amount of air can be selected within a wide range, but in order to reduce energy consumption and to effectively exert the cooling effect, it is preferable to use 225m3The bottle seedling room is taken as a reference, the total air delivery volume is 1100-1150m3/h。
In addition, in order to further improve the cooling effectPreferably at 225m3The bottle seedling room is used as a reference, the number of the air conveying openings is 6-10, and the area of each conveying opening is 0.2-0.4m2。
Finally, in order to avoid the contamination of the phalaenopsis explants by the delivered air, preferably, the method further comprises: filtering the air before the air is conveyed to the seedling chamber. Wherein, filter and adopt and just imitate, well effect and high-efficient three-layer filter screen to combine together and filter, the new trend filters the large granule dust through just imitating the filter screen, carries well effect filter screen after that, carries out high-efficient filter screen at last and delivers to the bottle seedling room through the air outlet, and the dust granule in the air that the control was delivered to the bottle seedling room is not more than 1 micron.
The present invention will be described in detail below by way of examples. In the following examples, the area of the seedling chamber was 90m2 and the height was 2.5 m.
In the following examples, the following treatments were required prior to propagation culture: explants (shoot tips) were deprived of apical bud dominance and basal cutin.
Example 1
Putting the explant into an SM culture medium for culture, and dividing each day into an illumination section and a black light section, wherein the illumination section is 19 hours, the black light section is 5 hours, and the black light section is between 10 and 3 points and a half of each day; the illumination section comprises a first illumination section of 9.5h and a second illumination section of 9.5h, and the illumination directions of the butterfly orchid explants in the proliferation stage in the first illumination section and the second illumination section are symmetrical; the illumination section conforms to: the illumination intensity is 1800lux, and the light source is an LED lamp with the power of 25W. Wherein the average temperature of the bottle seedling chamber of the illumination section is 29 ℃.
In the black light section, air is conveyed into the bottle seedling chamber; at 225m3The bottle seedling room is taken as a reference, 8 air conveying openings are formed, and the area of each conveying opening is 0.3m2Total air delivery of 1125m3H, leading the average temperature of the seedling chamber to be 24 ℃; the total days of culture was 30 days.
The culture results are shown in FIG. 1.
Example 2
Putting the explant into an SM culture medium for culture, and dividing each day into an illumination section and a black light section, wherein the illumination section is 18h, the black light section is 6h, and the black light section is between 10 and 4 points of each day; the illumination section comprises a first illumination section of 9h and a second illumination section of 9h, and the illumination directions of the butterfly orchid explants in the proliferation stage in the first illumination section and the second illumination section are symmetrical; the illumination section conforms to: the illumination intensity is 1500lux, and the light source is an LED lamp with the power of 28W. Wherein the average temperature of the bottle seedling chamber of the illumination section is 28 ℃.
In the black light section, air is conveyed into the bottle seedling chamber; at 225m3The bottle seedling room is taken as a reference, 8 air conveying openings are formed, and the area of each conveying opening is 0.3m2Total air delivery of 1125m3H, leading the average temperature of the seedling chamber to be 23 ℃; the total days of culture was 40 days.
Example 3
Putting the explant into an SM culture medium for culture, and dividing each day into an illumination section and a black light section, wherein the illumination section is 20 hours, the black light section is 4 hours, and the black light section is between 11 and 3 points of each day; the illumination section comprises a first illumination section of 10h and a second illumination section of 10h, and the illumination directions of the butterfly orchid explants in the proliferation stage in the first illumination section and the second illumination section are symmetrical; the illumination section conforms to: the illumination intensity is 2000lux, and the light source is an LED lamp with the power of 21W. Wherein the average temperature of the bottle seedling chamber of the illumination section is 30 ℃.
In the black light section, air is conveyed into the bottle seedling chamber; at 225m3The bottle seedling room is taken as a reference, 8 air conveying openings are formed, and the area of each conveying opening is 0.3m2Total air delivery of 1125m3H, leading the average temperature of the seedling chamber to be 25 ℃; (ii) a The total days of culture was 25 days.
Example 4
The method is carried out according to the embodiment 1, the only difference is that before air is conveyed to the bottle seedling chamber, the air is filtered, the combination of primary effect, intermediate effect and efficient three-layer filter screens is specifically adopted for filtering, the fresh air filters large-particle dust through the primary effect filter screens and then is conveyed to the intermediate effect filter screens, finally the efficient filter screens are carried out and are conveyed to the bottle seedling chamber through the air outlet, and the dust particles in the air conveyed to the bottle seedling chamber are controlled to be not more than 1 micron.
Comparative example 1
Culturing the explant in an SM culture medium, wherein the illumination condition is as follows: the illumination intensity is 1800lux, the light source is an LED lamp with the power of 25W, the illumination time and the dark condition are both 12h, and the illumination time is 7-19 points; once the temperature in the seedling chamber exceeds 28 ℃, an air conditioner is required to be started for cooling, and the temperature is maintained at 23-28 ℃; the total days of culture was 30 days.
The culture results are shown in FIG. 2.
Detection example 1
The proliferated explants obtained in the examples and comparative examples were tested, and the total electricity cost during the culture process in the examples and comparative examples was counted, and the specific results are shown in table 1, wherein the germination rate and the robustness were average values, and the germination rate was more than 99% (germination rate ═ number of germinated plants/number of inoculated plants × 100%).
TABLE 1
| Germination percentage (%) | Total electricity charge (RMB/Yuan) | |
| Example 1 | 99.3 | 3050 |
| Example 2 | 99.2 | 3650 |
| Examples3 | 99.1 | 2555 |
| Example 4 | 99.5 | 3065 |
| Comparative example 1 | 99.4 | 5125 |
As can be seen from the comparison of FIGS. 1 and 2, and the above table, in the examples and comparative examples, the germination effects of the shoots of examples 1 to 3 were equivalent to those of comparative example 1, and the germination effect of the shoot of example 4 was superior to that of the shoots of examples 1 to 3; compared with the comparative example 1, the energy consumption is far higher than that of the examples 1-4, so that the method provided by the application can achieve the purposes of robust buds, easy survival and no variation after division, does not need sufficient illumination like the rooting and strong seedling stages, and greatly reduces the consumed electric quantity compared with the comparative example 1.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
In addition, any combination of the various embodiments of the present invention is also possible, and the same should be considered as the disclosure of the present invention as long as it does not depart from the spirit of the present invention.
Claims (10)
1. A method for cultivating a differentiated bud of a butterfly orchid seedling by using extreme light is characterized by comprising the following steps: dividing each day into an illumination section and a black light section, wherein the illumination section is in 18-20h, the black light section is in 4-6h, and the black light section is in the highest outdoor temperature period under natural conditions; the illumination section comprises a first illumination section and a second illumination section, and the illumination directions of the butterfly orchid explants in the proliferation stage in the first illumination section and the second illumination section are symmetrical; and in the black light section, air is conveyed into the bottle seedling chamber.
2. The method of claim 1, wherein the first illumination session and the second illumination session are each independently 9-10 hours in time.
3. The method as claimed in claim 1, wherein in the first and second illumination sections, the illumination intensity is 1500-.
4. The method of claim 1, wherein the black light segment is between 10 and 4 points per day.
5. The method according to any one of claims 1 to 4, wherein the first illumination period and the second illumination period are both 10h in time, and the black illumination period is 4h in time.
6. The method of claim 5, wherein the black light segment is 11-3 dots per day.
7. The method according to any one of claims 1 to 4, wherein the total time of incubation in the method is 20 to 45 days.
8. The method according to any one of claims 1 to 4, characterized in that 225m is used3The bottle seedling room is taken as a reference, the total air delivery volume is 1100-1150m3/h。
9. The method of claim 8, wherein 225m is used3The bottle seedling room is used as a reference, the number of the air conveying openings is 6-10, and the area of each conveying opening is 0.2-0.4m2。
10. The method according to any one of claims 1-4, further comprising: filtering the air before the air is conveyed to the seedling chamber.
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