WO2015096463A1 - Phalaenopsis induction growth medium and phalaenopsis asexual reproduction method - Google Patents
Phalaenopsis induction growth medium and phalaenopsis asexual reproduction method Download PDFInfo
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- WO2015096463A1 WO2015096463A1 PCT/CN2014/082190 CN2014082190W WO2015096463A1 WO 2015096463 A1 WO2015096463 A1 WO 2015096463A1 CN 2014082190 W CN2014082190 W CN 2014082190W WO 2015096463 A1 WO2015096463 A1 WO 2015096463A1
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- phalaenopsis
- concentration
- induction medium
- induction
- gibberellin
- Prior art date
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- 241001505935 Phalaenopsis Species 0.000 title claims abstract description 84
- 230000006698 induction Effects 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000001963 growth medium Substances 0.000 title claims abstract 7
- 230000011681 asexual reproduction Effects 0.000 title abstract description 5
- 238000013465 asexual reproduction Methods 0.000 title abstract description 5
- 239000006259 organic additive Substances 0.000 claims abstract description 19
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 31
- IXORZMNAPKEEDV-SNTJWBGVSA-N LSM-6641 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@@]3(C)C(=O)O2 IXORZMNAPKEEDV-SNTJWBGVSA-N 0.000 claims description 21
- 229910052799 carbon Inorganic materials 0.000 claims description 13
- 235000015193 tomato juice Nutrition 0.000 claims description 13
- 229920001817 Agar Polymers 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical group O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims 13
- CGIDKJRJBMFXKV-UHFFFAOYSA-N 6-n'-benzylpurine-6,6-diamine Chemical compound N1=CN=C2N=CN=C2C1(N)NCC1=CC=CC=C1 CGIDKJRJBMFXKV-UHFFFAOYSA-N 0.000 claims 3
- AFWYNOWVYKOMGZ-UHFFFAOYSA-N 8-benzyl-7h-purine-2,6-diamine Chemical compound N=1C2=NC(N)=NC(N)=C2NC=1CC1=CC=CC=C1 AFWYNOWVYKOMGZ-UHFFFAOYSA-N 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 239000012452 mother liquor Substances 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 238000009396 hybridization Methods 0.000 abstract description 2
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 abstract 3
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 abstract 2
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 description 10
- -1 6-nonylaminoadenine Chemical compound 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 238000011081 inoculation Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000035755 proliferation Effects 0.000 description 5
- 229930024421 Adenine Natural products 0.000 description 4
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 4
- 229960000643 adenine Drugs 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 241000233855 Orchidaceae Species 0.000 description 3
- 244000127818 Phalaenopsis amabilis Species 0.000 description 3
- 235000020415 coconut juice Nutrition 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000005648 plant growth regulator Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241000286209 Phasianidae Species 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000021552 granulated sugar Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- MSSXOMSJDRHRMC-UHFFFAOYSA-N 9H-purine-2,6-diamine Chemical compound NC1=NC(N)=C2NC=NC2=N1 MSSXOMSJDRHRMC-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- FZSNGPRNPBZJGR-UHFFFAOYSA-N NNC1(C2=NC=NC2=NC=N1)N Chemical compound NNC1(C2=NC=NC2=NC=N1)N FZSNGPRNPBZJGR-UHFFFAOYSA-N 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 240000008708 Vanda coerulea Species 0.000 description 1
- 238000011276 addition treatment Methods 0.000 description 1
- HAMNKKUPIHEESI-UHFFFAOYSA-N aminoguanidine Chemical compound NNC(N)=N HAMNKKUPIHEESI-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
Definitions
- the invention relates to the field of plant tissue culture, in particular to a Phalaenopsis induction medium and a vegetative propagation method of Phalaenopsis. Background technique
- Phalaenopsis Bl. spp. is a tropical gas blue orchid with large flowers, long flowering period, beautiful flowers, rich color, beautiful flower shape, such as butterfly dancing, hence the name. It is known as the "Queen of Orchids" in the tropics and is one of the most popular orchids in recent years. Phalaenopsis is a single-flowered aerial orchid. Plants rarely develop lateral buds, and seeds are extremely difficult to germinate. They are routinely propagated and the proliferation rate is very slow. Therefore, many species are rapidly propagated by tissue culture.
- the object of the present invention is to provide a Phalaenopsis inducing medium and a method for vegetative propagation of Phalaenopsis, which can be used for inducing rapid propagation of Phalaenopsis in the internode of the butterfly orchid stem. Designed to provide a new method of asexual reproduction of Phalaenopsis.
- a Phalaenopsis induction medium wherein the Phalaenopsis induction medium is prepared by mixing 6-nonylaminoadenine BA, gibberellin GA 3 and organic additives with a standard N6 medium-based mother liquor; -
- the concentration of aminoguanidine BA is 0 ⁇ 10g/L
- the concentration of gibberellin GA 3 is 0 ⁇ 1.5 mg/L
- the concentration of organic additive is 0 ⁇ 300 g/L
- 6- ⁇ aminoadenine The content of BA and gibberellin GA 3 is non-zero.
- the phalaenopsis induction medium wherein the concentration of 6-nonamino adenine BA is 3 ⁇ 5 mg/L, the concentration of gibberellin GA 3 is l ⁇ 1.2 mg/L, and the concentration of organic additive is 150 ⁇ 180 g/L.
- the Phalaenopsis induction medium has a concentration of 6 mg/ammonium adenine BA of 5 mg/L, a concentration of gibberellin GA 3 of 1.2 mg/L, and an organic additive concentration of 150 g/L.
- the phalaenopsis induction medium wherein the phalaenopsis induction medium is added with agar, a carbon source and activated carbon; wherein the concentration of the agar is 7-9 g/L, and the concentration of the carbon source is 20-30 g/ L, the concentration of activated carbon is 1 ⁇ 3 g/L.
- the phalaenopsis induction medium wherein the organic additive is coconut juice or tomato juice.
- the phalaenopsis induction medium, wherein the organic additive is tomato juice.
- the Phalaenopsis induction medium wherein the carbon source is white sugar or sucrose.
- the phalaenopsis induction medium wherein the carbon source is white granulated sugar.
- a method for vegetative propagation of Phalaenopsis wherein the pheasant vegetative propagation method is an explant with a peduncle internode, and the phalaenopsis induction medium as described above is used as an induction medium.
- the pheasant vegetative propagation method wherein the phalaenopsis vegetative propagation method comprises the following steps:
- the present invention provides a Phalaenopsis inducing medium and a method for vegetative propagation of Phalaenopsis.
- the Phalaenopsis inducing medium can be used for inducing rapid propagation of Phalaenopsis in the internode of the butterfly orchid stem, which has low cost and protocorm-like bulbs.
- the induction rate is high, the induction time is short, and the growth rate is fast.
- the method for vegetative propagation of Phalaenopsis provided by the present invention is that the young peduncle is an explant and the phalaenopsis induction medium is used to rapidly propagate the phalaenopsis.
- the peduncle internode is the waste in the traditional tissue culture production. This method can not only make full use of the peduncle material, but also turn waste into treasure, saving cost, and Flowers that are in the lower part of the peduncle can still be used for cross-breeding without damaging the female parent. detailed description
- the present invention provides a Phalaenopsis Inducing Medium and a Phalaenopsis vegetative propagation method, and the present invention will be further described in detail below in order to clarify the purpose, technical solutions and effects of the present invention. It is understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
- the present invention provides a Phalaenopsis induction medium for inducing rapid propagation of Phalaenopsis in the internodes of the Phalaenopsis orchid.
- the Phalaenopsis induction medium is optimized by adding a plant growth regulator and an organic additive based on a standard N6 medium.
- the plant growth regulator is 6-nonylaminoadenine BA and gibberellin GA 3 .
- 6-nonylaminoadenine BA to the Phalaenopsis induction medium significantly promoted the induction and proliferation of protocorms in a certain range (0-5 mg/L, and the content is non-zero).
- the dominant factor of the protocorm-like stems induced by the peduncle can increase the induction rate and the late proliferation coefficient.
- Gibberellin GA 3 is a kind of phytohormone, which is mainly used to promote the growth of stems and leaves of plants, break the dormancy, promote heading, increase the number of male flowers in melons, induce parthenocarpy, and is rarely used in the induction of pedicels.
- the gibberellin GA 3 was creatively added to the Phalaenopsis induction medium, which significantly shortened the induction time and induced protocorm-like bulbs 10 to 15 days earlier than the no-addition treatment.
- the organic additive may be tomato juice or coconut juice, and in the solution of the present invention, preferably tomato juice.
- tomato juice is a natural organic additive that prevents the use of high concentrations of growth regulators and causes protocorm to mutate in proliferation.
- tomatoes are widely available all year round, everywhere, and cheap, reducing the cost of tissue culture.
- the Phalaenopsis induction medium is prepared by mixing 6-nonylaminoadenine BA, gibberellin GA 3 and an organic additive based on a standard N6 medium; wherein, 6-fluorenylamino The concentration of adenine BA is 0 ⁇ 10g/L, the concentration of gibberellin GA 3 is 0 ⁇ 1.5 mg/L, the concentration of organic additive is 0 ⁇ 300 g/L; and 6- ⁇ aminoadenine BA and red The content of the GA 3 is non-zero.
- the concentration of 6-mercaptoaminoadenine BA is 3 ⁇ 5 mg/L
- the concentration of gibberellin GA 3 is l ⁇ 1.2 mg/L
- the concentration of tomato juice is 150 ⁇ 180 g/L.
- the concentration of 6-mercaptoaminoadenine BA is 5 mg/L
- the concentration of gibberellin GA 3 is 1.2 mg/L
- the concentration of tomato juice is 150 g/L.
- the Phalaenopsis induction medium in this composition range has the highest induction rate, the shortest induction time, and the growth of protocorm-like bulbs is the best.
- the phalaenopsis induction medium may further contain agar, a carbon source and activated carbon; wherein the concentration of the agar is 7 to 9 g/L, the concentration of the carbon source is 20 to 30 g/L, and the concentration of the activated carbon It is 1 ⁇ 3 g/L.
- the carbon source may be white sugar or sucrose.
- white sugar is preferably used as a carbon source, because in the Phalaenopsis induction medium, white sugar is used as a carbon source in the induction rate and the induction time. Reduce costs without being affected.
- Agar can provide nutrients, moisture, and ventilating effect. The agar replaces the carrageenan in the traditional medium, and the preparation of the medium is reduced under the premise that the protocorm-inducing rate and the induction time are not affected. cost.
- the moth orchid induction medium provided by the present invention can be used in addition to various nutrient mother liquors in distilled water, and the rest can use tap water instead of distilled water, thereby indirectly simplifying the tissue culture procedure and cost.
- the invention also provides a method for vegetative propagation of Phalaenopsis, comprising the following steps:
- the method for asexual reproduction of Phalaenopsis adopts the young peduncle as an explant, which not only fully utilizes the peduncle material, but also does not damage the female parent, and the flower at the lower part of the peduncle can also be used for hybridization;
- the above-mentioned phalaenopsis induction medium is used as an induction medium, which has the advantages of low cost, high protocorm-inducing rate, short induction time, and fast growth rate.
- the base mother liquor of the Phalaenopsis induction medium was prepared according to the composition and concentration of the standard N6 medium, and was dispensed into nine triangular flasks and labeled separately.
- the No. 1 to No. 8 flasks were experimentally added to the Phalaenopsis induction medium supplemented with plant growth regulators and organic additives, and the No. 9 flask was a medium control group in which only agar, white granulated sugar and activated carbon were added.
- Example 2 Inoculation and culture of peduncle internode Inoculation in a sterile ultra-clean workbench, using a sterilized scalpel to cut the incision in contact with the disinfectant at both ends of the peduncle, taking the young part between the two pedicel sections, and cutting into 2 ⁇ 3mm sections, quickly inoculation
- the stopper was sealed with a sterilized stopper.
- the culture flask After inoculation, the culture flask is placed in the artificial culture chamber.
- the environmental parameters of the culture flask are as follows: Culture conditions: temperature 25 ⁇ 1 °C:, light intensity 1500 ⁇ 18001x, light and dark alternate 12h/12h, culture for 50 days, The induction rate, induction time and growth of the protocorm-like bulbs in the culture flask were recorded (see Table 1 for experimental data).
- the induction rate effect and growth condition of the induction medium of the present invention are better, especially when a certain amount (3 ⁇ 5 mg/L) of 6- ⁇ is added.
- the amino adenine BA Induction medium No. 1, No. 2, No. 5, No. 7, No. 8
- the induction rate not only did not decrease, but also increased; in the control group, no 6-mercaptoaminoadenine BA was added.
- the peduncle did not germinate during the internodes, and no protocorm-like bulbs were induced.
- 6-aminoamino adenine BA can effectively increase the induction rate of protocorm-like bulbs, which is the key to the success of pedicel internodes.
- Adding tomato juice has a certain promoting effect on the growth and development of protocorm-like bulbs, but the effect on the induction rate The noise is not obvious.
- gibberellin GA 3 can significantly shorten the induction time without affecting the induction rate, 10 to 15 days earlier than the non-addition.
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
Disclosed are a Phalaenopsis induction growth medium and a Phalaenopsis asexual reproduction method. The Phalaenopsis induction growth medium is prepared by mixing a standard N6 growth medium serving as a base mother liquor with 6-benzylaminopurine (BA), gibberellin A3 (GA3), and an organic additive added. The Phalaenopsis induction growth medium can be used for inducting a Phalaenopsis peduncle internode portion to rapidly reproduce Phalaenopsis and has a high protocorm-like body induction rate and a short induction time. Also provided in the present invention is a Phalaenopsis asexual reproduction method, which uses newly-budded peduncle internode as an explant and employs the Phalaenopsis induction growth medium. The method not only allows for full utilization of a peduncle material, but also causes no damage to a mother plant, where a flower located at lower parts of the peduncle can still be used for hybridization.
Description
一种蝴蝶兰诱导培养基及蝴蝶兰无性繁殖方法 技术领域 Phalaenopsis induction medium and clonal propagation method of Phalaenopsis
本发明涉及植物组织培养领域， 尤其涉及一种蝴蝶兰诱导培养基及蝴 蝶兰无性繁殖方法。 背景技术 The invention relates to the field of plant tissue culture, in particular to a Phalaenopsis induction medium and a vegetative propagation method of Phalaenopsis. Background technique
蝴蝶兰（Phalaenopsis Bl. spp. )属热带气生兰， 其花大， 花期长， 花 色艳丽， 色泽丰富， 花形美丽别致， 如蝴蝶翩翩起舞， 因而得名。 在热带 兰中有 "兰花皇后" 之美称， 是近年来最受欢迎的洋兰之一。 蝴蝶兰是单 茎性气生兰， 植株很少发育侧芽， 且种子极难萌发， 对其进行常规性的繁 殖， 增殖速度很慢， 故多釆用组织培养的方法对其进行快速繁殖。 目前蝴 蝶兰组培工厂化生产大多使用花梗腋芽诱导不定芽， 废弃花梗节间部分， 这样不仅损伤母本， 还浪费花梗节间部分。 Phalaenopsis Bl. spp. is a tropical gas blue orchid with large flowers, long flowering period, beautiful flowers, rich color, beautiful flower shape, such as butterfly dancing, hence the name. It is known as the "Queen of Orchids" in the tropics and is one of the most popular orchids in recent years. Phalaenopsis is a single-flowered aerial orchid. Plants rarely develop lateral buds, and seeds are extremely difficult to germinate. They are routinely propagated and the proliferation rate is very slow. Therefore, many species are rapidly propagated by tissue culture. At present, most of the factory production of butterfly cultivars is to use the peduncle bud to induce adventitious buds, and to abandon the internodes of the peduncle, which not only damages the female parent, but also wastes the internodes of the peduncle.
因此， 现有技术还有待发展。 发明内容 Therefore, the prior art has yet to be developed. Summary of the invention
鉴于上述现有技术的不足， 本发明的目的在于提供一种蝴蝶兰诱导培 养基及蝴蝶兰无性繁殖方法， 所述蝴蝶兰诱导培养基可用于诱导蝴蝶兰花 梗节间部分快速繁殖蝴蝶兰， 旨在提供一种新的蝴蝶兰无性繁殖方法。 In view of the above-mentioned deficiencies of the prior art, the object of the present invention is to provide a Phalaenopsis inducing medium and a method for vegetative propagation of Phalaenopsis, which can be used for inducing rapid propagation of Phalaenopsis in the internode of the butterfly orchid stem. Designed to provide a new method of asexual reproduction of Phalaenopsis.
本发明的技术方案如下： The technical solution of the present invention is as follows:
一种蝴蝶兰诱导培养基， 其中， 所述蝴蝶兰诱导培养基是以标准 N6培 养基为基础母液， 加入 6-卞氨基腺嘌呤 BA、 赤霉素 GA3和有机添加物混 合制成； 6-卞氨基腺嘌呤 BA的浓度为 0~10g/L，赤霉素 GA3的浓度为 0~1.5 mg/L, 有机添加物的浓度为 0~300 g/L; 且 6-卞氨基腺嘌呤 BA和赤霉素 GA3的含量非零。
所述的蝴蝶兰诱导培养基， 其中， 6-卞氨基腺嘌呤 BA的浓度为 3~5mg/L， 赤霉素 GA3的浓度为 l~1.2 mg/L， 有机添加物的浓度为 150~180 g/L。 A Phalaenopsis induction medium, wherein the Phalaenopsis induction medium is prepared by mixing 6-nonylaminoadenine BA, gibberellin GA 3 and organic additives with a standard N6 medium-based mother liquor; - The concentration of aminoguanidine BA is 0~10g/L, the concentration of gibberellin GA 3 is 0~1.5 mg/L, the concentration of organic additive is 0~300 g/L; and 6-卞aminoadenine The content of BA and gibberellin GA 3 is non-zero. The phalaenopsis induction medium, wherein the concentration of 6-nonamino adenine BA is 3~5 mg/L, the concentration of gibberellin GA 3 is l~1.2 mg/L, and the concentration of organic additive is 150~ 180 g/L.
所述的蝴蝶兰诱导培养基，其中， 6-卞氨基腺嘌呤 BA的浓度为 5 mg/L, 赤霉素 GA3的浓度为 1.2 mg/L， 有机添加物的浓度为 150 g/L。 The Phalaenopsis induction medium has a concentration of 6 mg/ammonium adenine BA of 5 mg/L, a concentration of gibberellin GA 3 of 1.2 mg/L, and an organic additive concentration of 150 g/L.
所述的蝴蝶兰诱导培养基， 其中， 所述蝴蝶兰诱导培养基中添加有琼 脂、碳源和活性炭； 其中， 琼脂的浓度为 7~9g/L，碳源的浓度是 20~30 g/L， 活性炭的浓度为 1~3 g/L。 The phalaenopsis induction medium, wherein the phalaenopsis induction medium is added with agar, a carbon source and activated carbon; wherein the concentration of the agar is 7-9 g/L, and the concentration of the carbon source is 20-30 g/ L, the concentration of activated carbon is 1~3 g/L.
所述的蝴蝶兰诱导培养基， 其中， 所述有机添加物为椰子汁或番茄汁。 所述的蝴蝶兰诱导培养基， 其中， 所述有机添加物为番茄汁。 The phalaenopsis induction medium, wherein the organic additive is coconut juice or tomato juice. The phalaenopsis induction medium, wherein the organic additive is tomato juice.
所述的蝴蝶兰诱导培养基， 其中， 所述碳源为白砂糖或蔗糖。 The Phalaenopsis induction medium, wherein the carbon source is white sugar or sucrose.
所述的蝴蝶兰诱导培养基， 其中， 所述碳源为白砂糖。 The phalaenopsis induction medium, wherein the carbon source is white granulated sugar.
一种蝴蝶兰无性繁殖方法， 其中， 所述蝴蝶兰无性繁殖方法是以花梗 节间为外植体， 釆用如上任一所述的蝴蝶兰诱导培养基作为诱导培养基。 A method for vegetative propagation of Phalaenopsis, wherein the pheasant vegetative propagation method is an explant with a peduncle internode, and the phalaenopsis induction medium as described above is used as an induction medium.
所述的蝴蝶兰无性繁殖方法， 其中， 所述蝴蝶兰无性繁殖方法包括以 下步骤： The pheasant vegetative propagation method, wherein the phalaenopsis vegetative propagation method comprises the following steps:
取蝴蝶兰两花梗节之间的幼嫩部分， 切成 2~3mm的切段， 接种于盛装 有所述蝴蝶兰诱导培养基的培养瓶中， 封口； 将培养瓶置于培养室中， 在 温度 25 ± 1 °C：、光照强度 1500~18001x、光暗交替 12h/12h条件下，培养 42~50 天。 Take the young part between the two peduncles of Phalaenopsis, cut into 2~3mm sections, inoculate the culture flask containing the Phalaenopsis induction medium, and seal; put the culture bottle in the culture room, The temperature is 25 ± 1 °C: the light intensity is 1500~18001x, and the light and dark are alternately 12h/12h, and the culture is carried out for 42~50 days.
有益效果： 本发明所提供一种蝴蝶兰诱导培养基及蝴蝶兰无性繁殖方 法， 所述蝴蝶兰诱导培养基可用于诱导蝴蝶兰花梗节间部分快速繁殖蝴蝶 兰， 具有成本低， 类原球茎诱导率高， 诱导时间短， 生长速度快等优点。 本发明所提供的蝴蝶兰无性繁殖方法， 是以幼嫩的花梗节间为外植体并釆 用所述蝴蝶兰诱导培养基快速繁殖蝴蝶兰。 花梗节间是传统组培生产中的 废料， 釆用此方法不仅能充分利用花梗材料， 变废为宝， 节约成本， 而且
不损伤母本， 处于花梗较低部位的花仍可用于杂交。 具体实施方式 Advantageous Effects: The present invention provides a Phalaenopsis inducing medium and a method for vegetative propagation of Phalaenopsis. The Phalaenopsis inducing medium can be used for inducing rapid propagation of Phalaenopsis in the internode of the butterfly orchid stem, which has low cost and protocorm-like bulbs. The induction rate is high, the induction time is short, and the growth rate is fast. The method for vegetative propagation of Phalaenopsis provided by the present invention is that the young peduncle is an explant and the phalaenopsis induction medium is used to rapidly propagate the phalaenopsis. The peduncle internode is the waste in the traditional tissue culture production. This method can not only make full use of the peduncle material, but also turn waste into treasure, saving cost, and Flowers that are in the lower part of the peduncle can still be used for cross-breeding without damaging the female parent. detailed description
本发明提供一种蝴蝶兰诱导培养基及蝴蝶兰无性繁殖方法， 为使本发 明的目的、 技术方案及效果更加清楚、 明确， 以下对本发明进一步详细说 明。 应当理解， 此处所描述的具体实施例仅仅用以解释本发明， 并不用于 限定本发明。 The present invention provides a Phalaenopsis Inducing Medium and a Phalaenopsis vegetative propagation method, and the present invention will be further described in detail below in order to clarify the purpose, technical solutions and effects of the present invention. It is understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
本发明提供一种蝴蝶兰诱导培养基， 用于诱导蝴蝶兰花梗节间部分快 速繁殖蝴蝶兰。 所述蝴蝶兰诱导培养基， 是以标准 N6培养基为基础母液， 加入植物生长调节剂和有机添加物优化制得的。 The present invention provides a Phalaenopsis induction medium for inducing rapid propagation of Phalaenopsis in the internodes of the Phalaenopsis orchid. The Phalaenopsis induction medium is optimized by adding a plant growth regulator and an organic additive based on a standard N6 medium.
其中， 所述植物生长调节剂为 6-卞氨基腺嘌呤 BA和赤霉素 GA3。 在 所述蝴蝶兰诱导培养基中添加 6-卞氨基腺嘌呤 BA，在一定范围（0~5mg/L， 且含量非零） 内对类原球茎的诱导及增殖有显著的促进作用， 是使花梗节 间诱导成类原球茎的主导因子， 可提高诱导率和后期的增殖系数。 赤霉素 GA3是一种植物激素， 主要用于促进植物茎叶生长， 打破休眠， 促进抽穗， 增加瓜类的雄花数， 诱导单性结实， 少见用于花梗诱导中。 在所述蝴蝶兰 诱导培养基中创造性地加入了赤霉素 GA3， 能明显地缩短诱导时间， 比不 添加处理提前 10~15天诱导出类原球茎。 Wherein, the plant growth regulator is 6-nonylaminoadenine BA and gibberellin GA 3 . Adding 6-nonylaminoadenine BA to the Phalaenopsis induction medium significantly promoted the induction and proliferation of protocorms in a certain range (0-5 mg/L, and the content is non-zero). The dominant factor of the protocorm-like stems induced by the peduncle can increase the induction rate and the late proliferation coefficient. Gibberellin GA 3 is a kind of phytohormone, which is mainly used to promote the growth of stems and leaves of plants, break the dormancy, promote heading, increase the number of male flowers in melons, induce parthenocarpy, and is rarely used in the induction of pedicels. The gibberellin GA 3 was creatively added to the Phalaenopsis induction medium, which significantly shortened the induction time and induced protocorm-like bulbs 10 to 15 days earlier than the no-addition treatment.
所述有机添加物可以为番茄汁或椰子汁， 在本发明方案中优选为番茄 汁。 在所述蝴蝶兰诱导培养基中添加番茄汁， 能有效地提高蝴蝶兰类原球 茎的健壮度， 减少后期增殖时的褐化和枯死。 番茄汁是一种天然的有机添 加剂， 能避免高浓度生长调节物质的使用导致类原球茎在增殖中发生变异。 同时相较于起相似作用的椰子汁， 番茄一年四季均有大量供应， 随处可见， 且价格便宜， 降低了组培成本。 The organic additive may be tomato juice or coconut juice, and in the solution of the present invention, preferably tomato juice. The addition of tomato juice to the Phalaenopsis induction medium can effectively improve the robustness of the original bulb of the Phalaenopsis, and reduce the browning and death of the late proliferation. Tomato juice is a natural organic additive that prevents the use of high concentrations of growth regulators and causes protocorm to mutate in proliferation. At the same time, compared with similarly used coconut juice, tomatoes are widely available all year round, everywhere, and cheap, reducing the cost of tissue culture.
综上， 所述蝴蝶兰诱导培养基， 是以标准 N6培养基为基础母液， 加入 6-卞氨基腺嘌呤 BA、 赤霉素 GA3和有机添加物混合制成； 其中， 6-卞氨基
腺嘌呤 BA的浓度为 0~10g/L， 赤霉素 GA3的浓度为 0~1.5 mg/L， 有机添 加物的浓度为 0~300 g/L;且 6-卞氨基腺嘌呤 BA和赤霉素 GA3的含量非零。 In summary, the Phalaenopsis induction medium is prepared by mixing 6-nonylaminoadenine BA, gibberellin GA 3 and an organic additive based on a standard N6 medium; wherein, 6-fluorenylamino The concentration of adenine BA is 0~10g/L, the concentration of gibberellin GA 3 is 0~1.5 mg/L, the concentration of organic additive is 0~300 g/L; and 6-卞aminoadenine BA and red The content of the GA 3 is non-zero.
优选地， 所述蝴蝶兰诱导培养基中， 6-卞氨基腺嘌呤 BA的浓度为 3~5mg/L， 赤霉素 GA3的浓度为 l~1.2 mg/L， 番茄汁的浓度为 150~180 g/L。 釆用此组成范围内的蝴蝶兰诱导培养基， 其诱导率较高， 诱导时间较短， 类原球茎的生长发育较为良好。 Preferably, in the Phalaenopsis induction medium, the concentration of 6-mercaptoaminoadenine BA is 3~5 mg/L, the concentration of gibberellin GA 3 is l~1.2 mg/L, and the concentration of tomato juice is 150~ 180 g/L. Using the Phalaenopsis induction medium in this composition range, the induction rate is higher, the induction time is shorter, and the protocorm-like growth and development is better.
最为优选地， 所述蝴蝶兰诱导培养基中， 6-卞氨基腺嘌呤 BA的浓度为 5 mg/L, 赤霉素 GA3的浓度为 1.2 mg/L， 番茄汁的浓度为 150 g/L。 釆用此 组成范围内的蝴蝶兰诱导培养基， 其诱导率最高， 诱导时间最短， 且类原 球茎的生长发育状况最好。 Most preferably, the concentration of 6-mercaptoaminoadenine BA is 5 mg/L, the concentration of gibberellin GA 3 is 1.2 mg/L, and the concentration of tomato juice is 150 g/L. . The Phalaenopsis induction medium in this composition range has the highest induction rate, the shortest induction time, and the growth of protocorm-like bulbs is the best.
进一步地， 所述蝴蝶兰诱导培养基中， 还可以添加有琼脂、 碳源和活 性炭； 其中， 琼脂的浓度为 7~9g/L， 碳源的浓度是 20~30 g/L， 活性炭的浓 度为 1~3 g/L。 Further, the phalaenopsis induction medium may further contain agar, a carbon source and activated carbon; wherein the concentration of the agar is 7 to 9 g/L, the concentration of the carbon source is 20 to 30 g/L, and the concentration of the activated carbon It is 1~3 g/L.
其中， 在所述蝴蝶兰诱导培养基中添加 0.2%左右的活性炭， 可以抑制 褐化现象， 提高类原球茎的成活率。 所述碳源可以为白砂糖或蔗糖， 在本 发明方案中， 优选为白砂糖作为碳源， 因为在所述蝴蝶兰诱导培养基中釆 用白砂糖作为碳源， 在对诱导率及诱导时间不受影响的前提下降低了成本。 而琼脂能够为植株提供养分、 水分， 并能起到透气的效用， 用琼脂代替传 统培养基中的卡拉胶， 在类原球茎诱导率以及诱导时间不受影响的前提下 降低了培养基的制备成本。 Among them, adding about 0.2% of activated carbon to the Phalaenopsis induction medium can suppress browning and increase the survival rate of the protocorm-like bulb. The carbon source may be white sugar or sucrose. In the solution of the present invention, white sugar is preferably used as a carbon source, because in the Phalaenopsis induction medium, white sugar is used as a carbon source in the induction rate and the induction time. Reduce costs without being affected. Agar can provide nutrients, moisture, and ventilating effect. The agar replaces the carrageenan in the traditional medium, and the preparation of the medium is reduced under the premise that the protocorm-inducing rate and the induction time are not affected. cost.
本发明所提供的蝴蝶兰诱导培养基， 除各种营养元素母液釆用蒸馏水 配制以外， 其余均可釆用自来水代替蒸馏水， 从而间接简化了组培程序及 成本。 The moth orchid induction medium provided by the present invention can be used in addition to various nutrient mother liquors in distilled water, and the rest can use tap water instead of distilled water, thereby indirectly simplifying the tissue culture procedure and cost.
本发明中还提供一种蝴蝶兰无性繁殖方法， 包括以下步骤： The invention also provides a method for vegetative propagation of Phalaenopsis, comprising the following steps:
取蝴蝶兰两花梗节之间的幼嫩部分， 切成 2~3mm的切段， 迅速接种于 盛装有上述蝴蝶兰诱导培养基的培养瓶中， 用瓶塞封口； 接种后， 将培养
瓶置于培养室中，在温度 25 ± 1 °C、光照强度 1500~18001x、光暗交替 12h/12h 条件下， 培养 42~50天。 Take the young part between the two peduncles of Phalaenopsis, cut into 2~3mm sections, quickly inoculate the flask containing the above-mentioned Phalaenopsis induction medium, and seal with a stopper; after inoculation, it will be cultured. The bottle was placed in a culture chamber and cultured for 42 to 50 days at a temperature of 25 ± 1 °C, a light intensity of 1500 to 18001x, and an alternating light and darkness of 12h/12h.
本发明所提供的蝴蝶兰无性繁殖方法， 以幼嫩的花梗节间为外植体， 不仅充分利用了花梗材料， 而且不损伤母本， 处于花梗较低部位的花还可 用于杂交； 另外， 釆用上述蝴蝶兰诱导培养基作为诱导培养基， 具有成本 低， 类原球茎诱导率高， 诱导时间短， 生长速度快等优点。 The method for asexual reproduction of Phalaenopsis provided by the invention adopts the young peduncle as an explant, which not only fully utilizes the peduncle material, but also does not damage the female parent, and the flower at the lower part of the peduncle can also be used for hybridization; The above-mentioned phalaenopsis induction medium is used as an induction medium, which has the advantages of low cost, high protocorm-inducing rate, short induction time, and fast growth rate.
下面通过实施例对本发明作进一步说明， 但本发明不限于以下实施例 的举例。 The invention is further illustrated by the following examples, but the invention is not limited to the examples of the following examples.
实施例 1: 配制诱导培养基 Example 1: Preparation of induction medium
( 1 )根据标准 N6培养基的成分和浓度配制所述蝴蝶兰诱导培养基的 基础母液， 并分装于 9个三角瓶中， 并分别标号。 1号〜 8号三角瓶为本发 明添加有植物生长调节剂和有机添加物的蝴蝶兰诱导培养基实验组， 9号瓶 为仅添加琼脂、 白砂糖和活性炭的培养基对照组。 (1) The base mother liquor of the Phalaenopsis induction medium was prepared according to the composition and concentration of the standard N6 medium, and was dispensed into nine triangular flasks and labeled separately. The No. 1 to No. 8 flasks were experimentally added to the Phalaenopsis induction medium supplemented with plant growth regulators and organic additives, and the No. 9 flask was a medium control group in which only agar, white granulated sugar and activated carbon were added.
( 2 )根据表 1， 将 6-卞氨基腺嘌呤 BA母液、 番茄汁和活性炭加入各 三角瓶中与 N6基础母液混合至所需浓度。 (2) According to Table 1, 6-nonamino adenine BA mother liquor, tomato juice and activated carbon were added to each flask and mixed with the N6 base mother liquor to the desired concentration.
( 3 )取一定量自来水（代替蒸馏水） 煮沸， 根据表 1中的浓度， 用电 子天平秤取琼脂及白砂糖， 将琼脂倒入沸水中煮化， 接着倒入白砂糖， 溶 化后， 将琼脂液分别分装在 1号〜 9号三角瓶中， 定容至 1L， 加盖灭菌。 (3) Take a certain amount of tap water (instead of distilled water) and boil. According to the concentration in Table 1, weigh agar and white sugar with an electronic balance, pour the agar into boiling water, cook it, then pour in white sugar, dissolve it, and then agar The liquid was separately packed in a No. 1 to No. 9 flask, and the volume was adjusted to 1 L, and the lid was sterilized.
( 4 )将赤霉素 GA3小瓶封口用 75%的酒精消毒后， 用一次性医用注射 器注入乙醇配成 l mg/L的赤霉素 GA3母液， 用过滤灭菌器进行过滤灭菌， 待用。 (4) After clarifying the gibberellin GA 3 vial with 75% alcohol, injecting ethanol into a 1 mg/L gibberellin GA 3 mother liquor with a disposable medical syringe, and filtering and sterilizing with a filter sterilizer. stand-by.
( 5 )待灭菌后培养基的温度冷却至 40 ~ 50°C时， 在无菌的超净工作台 上根据表 1在 1号〜 8号瓶中加入不同浓度的赤霉素 GA3， 混匀 , 分装到培 养瓶中， 得类原球茎诱导培养基， 即本发明所述蝴蝶兰诱导培养基。 (5) When the temperature of the medium to be sterilized is cooled to 40 ~ 50 °C, different concentrations of gibberellin GA 3 are added to the bottles of No. 1 to No. 8 according to Table 1 on a sterile ultra-clean workbench. The mixture is mixed and dispensed into a culture flask to obtain a protocorm-inducing medium, that is, a Phalaenopsis induction medium according to the present invention.
实施例 2: 花梗节间的接种及培养
在无菌超净工作台中进行接种， 用已消毒的手术刀切除花梗两端与消 毒剂接触的切口， 取两花梗节之间的幼嫩部分， 分切成 2~3mm的切段，迅 速接种至实施例 1中制备得到的各诱导培养基中， 接种后用已消毒的瓶塞 封口。 Example 2: Inoculation and culture of peduncle internode Inoculation in a sterile ultra-clean workbench, using a sterilized scalpel to cut the incision in contact with the disinfectant at both ends of the peduncle, taking the young part between the two pedicel sections, and cutting into 2~3mm sections, quickly inoculation To each of the induction media prepared in Example 1, after inoculation, the stopper was sealed with a sterilized stopper.
接种后， 将培养瓶置于人工培养室中， 培养瓶所处环境参数如下： 培养条件： 温度 25 ± 1 °C：、 光照强度 1500~18001x、 光暗交替 12h/12h 培养 50天后， 对各培养瓶中类原球茎的诱导率、 诱导时间及生长情况 进行记录（实验数据见表 1 ) After inoculation, the culture flask is placed in the artificial culture chamber. The environmental parameters of the culture flask are as follows: Culture conditions: temperature 25 ± 1 °C:, light intensity 1500~18001x, light and dark alternate 12h/12h, culture for 50 days, The induction rate, induction time and growth of the protocorm-like bulbs in the culture flask were recorded (see Table 1 for experimental data).
表 1诱导培养基各成分使用浓度及实验结果数据 Table 1 Induction medium concentration and experimental results data
由表 1可以看出， 在同样的诱导培养条件下， 釆用本发明诱导培养基 的诱导率效果和生长状况都比较好， 特别是在添加一定量（3~5mg/L ) 的 6-卞氨基腺嘌呤 BA ( 1号、 2号、 5号、 7号、 8号诱导培养基） 时， 诱导 率不仅没有降低，反而还有所提高；对照组中没有添加 6-卞氨基腺嘌呤 BA 所有花梗节间均不萌动， 没有诱导出类原球茎， 证明添加 6-卞氨基腺嘌呤 BA能有效地提高类原球茎的诱导率， 是花梗节间是否能诱导成功的关键。 添加番茄汁， 对类原球茎的生长发育有一定的促进作用， 但对诱导率的影
响不明显。 赤霉素 GA3的添加， 在不影响诱导率的情况下， 能明显地缩短 诱导时间， 比不添加项提前 10~15天。 It can be seen from Table 1 that under the same induction culture conditions, the induction rate effect and growth condition of the induction medium of the present invention are better, especially when a certain amount (3~5 mg/L) of 6-卞 is added. When the amino adenine BA (Induction medium No. 1, No. 2, No. 5, No. 7, No. 8), the induction rate not only did not decrease, but also increased; in the control group, no 6-mercaptoaminoadenine BA was added. The peduncle did not germinate during the internodes, and no protocorm-like bulbs were induced. It was proved that the addition of 6-aminoamino adenine BA can effectively increase the induction rate of protocorm-like bulbs, which is the key to the success of pedicel internodes. Adding tomato juice has a certain promoting effect on the growth and development of protocorm-like bulbs, but the effect on the induction rate The noise is not obvious. The addition of gibberellin GA 3 can significantly shorten the induction time without affecting the induction rate, 10 to 15 days earlier than the non-addition.
应当理解的是， 本发明的应用不限于上述的举例， 对本领域普通技术 人员来说， 可以根据上述说明加以改进或变换， 所有这些改进和变换都应 属于本发明所附权利要求的保护范围。
It is to be understood that the application of the present invention is not limited to the above-described examples, and those skilled in the art can make modifications and changes in accordance with the above description, all of which are within the scope of the appended claims.
Claims
1、 一种蝴蝶兰诱导培养基， 其特征在于， 所述蝴蝶兰诱导培养基是以 标准 N6培养基为基础母液， 加入 6-卞氨基腺 BA、 赤霉素 GA3和有机添加 物混合制成； 6-卞氨基腺嘌呤 BA的浓度为 0~10g/L， 赤霉素 GA3的浓度 为 0~1.5 mg/L， 有机添加物的浓度为 0~300 g/L; 其中， 6-卞氨基腺嘌呤 BA 含量和赤霉素 GA3的非零。 1. A Phalaenopsis induction medium, characterized in that, the Phalaenopsis induction medium is a mixture of standard N6 culture medium and the addition of 6-benzylaminoadene BA, gibberellin GA 3 and organic additives. into; the concentration of 6-benzylaminoadenine BA is 0~10g/L, the concentration of gibberellin GA 3 is 0~1.5 mg/L, and the concentration of organic additives is 0~300 g/L; among them, 6- The content of benzyl aminoadenine BA and gibberellin GA 3 is non-zero.
2、 根据权利要求 1所述的蝴蝶兰诱导培养基， 其特征在于， 6-卞氨基 腺嘌呤 BA的浓度为 3~5mg/L， 赤霉素 GA3的浓度为 1~1.2 mg/L, 有机添 加物的浓度为 150~180 g/L。 2. Phalaenopsis induction medium according to claim 1, characterized in that the concentration of 6-benzylaminoadenine BA is 3~5 mg/L, and the concentration of gibberellin GA 3 is 1~1.2 mg/L, The concentration of organic additives is 150~180 g/L.
3、 根据权利要求 1所述的蝴蝶兰诱导培养基， 其特征在于， 6-卞氨基 腺嘌呤 BA的浓度为 5 mg/L, 赤霉素 GA3的浓度为 1.2 mg/L, 有机添加物 的浓度为 150 g/L。 3. Phalaenopsis induction medium according to claim 1, characterized in that the concentration of 6-benzylaminoadenine BA is 5 mg/L, the concentration of gibberellin GA 3 is 1.2 mg/L, and organic additives The concentration is 150 g/L.
4、 根据权利要求 1~3任一所述的蝴蝶兰诱导培养基， 其特征在于， 所 述蝴蝶兰诱导培养基中添加有琼脂、 碳源和活性炭； 其中， 琼脂的浓度为 7~9g/L， 碳源的浓度是 20~30 g/L， 活性炭的浓度为 1~3 g/L。 4. Phalaenopsis induction medium according to any one of claims 1 to 3, characterized in that agar, carbon source and activated carbon are added to the Phalaenopsis induction medium; wherein, the concentration of agar is 7~9g/ L, the concentration of carbon source is 20~30 g/L, and the concentration of activated carbon is 1~3 g/L.
5、 根据权利要求 4所述的蝴蝶兰诱导培养基， 其特征在于， 所述有机 添加物为耶子汁或番茄汁。 5. The Phalaenopsis induction medium according to claim 4, characterized in that the organic additive is tomato juice or tomato juice.
6、 根据权利要求 4所述的蝴蝶兰诱导培养基， 其特征在于， 所述有机 添力。物为番茄汁。 6. The Phalaenopsis induction medium according to claim 4, characterized in that the organic additive. The substance is tomato juice.
7、 根据权利要求 4所述的蝴蝶兰诱导培养基， 其特征在于， 所述碳源 为白砂糖或蔗糖。 7. The Phalaenopsis induction medium according to claim 4, characterized in that the carbon source is white sugar or sucrose.
8、 根据权利要求 4所述的蝴蝶兰诱导培养基， 其特征在于， 所述碳源 为白 -糖。 8. The Phalaenopsis induction medium according to claim 4, characterized in that the carbon source is white sugar.
9、 一种蝴蝶兰无性繁殖方法， 其特征在于， 所述蝴蝶兰无性繁殖方法 是以花梗节间为外植体， 釆用如权利要求 1~8任一所述的蝴蝶兰诱导培养 基作为诱导培养基。
9. A vegetative propagation method of Phalaenopsis, characterized in that the vegetative propagation method of Phalaenopsis is to use the pedicel internodes as explants, and use the Phalaenopsis induction medium as described in any one of claims 1 to 8 as the explant. Induction medium.
10、 根据权利要求 9所述的蝴蝶兰无性繁殖方法， 其特征在于， 所蝴 蝶兰无性繁殖方法包括以下步骤： 10. The vegetative propagation method of Phalaenopsis according to claim 9, characterized in that the vegetative propagation method of Phalaenopsis includes the following steps:
取蝴蝶兰两花梗节之间的幼嫩部分， 切成 2~3mm的切段， 接种于盛装 有所述蝴蝶兰诱导培养基的培养瓶中， 封口； 将培养瓶置于培养室中， 在 温度 25 ± 1 °C：、光照强度 1500~18001x、光暗交替 12h/12h条件下，培养 42~50 天。
Take the young part between the two pedicel nodes of Phalaenopsis, cut it into 2~3mm sections, inoculate it into a culture bottle containing the Phalaenopsis induction medium, and seal it; place the culture bottle in the culture room, and Temperature 25 ± 1 °C: Light intensity 1500~18001x, light and dark alternation 12h/12h, culture for 42~50 days.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109618919A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | A kind of cultural method improving exhibition shape cherry iris flowering rate |
| CN110999792A (en) * | 2019-12-30 | 2020-04-14 | 临沧市云瑞堂生物科技有限公司 | Multi-bud culture method for anoectochilus roxburghii |
| CN113229147A (en) * | 2021-06-07 | 2021-08-10 | 芜湖东源新农村开发股份有限公司 | Method for cultivating differentiated bud of butterfly orchid seedling by using extreme illumination |
| CN115104535A (en) * | 2022-07-20 | 2022-09-27 | 浙江农林大学 | Method for regenerating phalaenopsis into complete plant by taking leaves as explants |
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| CN104604687B (en) * | 2015-01-29 | 2017-07-18 | 赤峰市农牧科学研究院 | Utilize the method for cutting the induction Multiple Buds propagating quickly butterfly orchid of the bennet stem section after bud |
| CN106489461A (en) * | 2015-09-07 | 2017-03-15 | 湖南瑞利农业发展有限公司 | A kind of breeding method of iris bennet Seedling |
| CN106069778A (en) * | 2016-07-31 | 2016-11-09 | 张玉薇 | The method for tissue culture of Herba Cymbidii Goeringii |
| CN106234218A (en) * | 2016-07-31 | 2016-12-21 | 张玉薇 | Herba Cymbidii Goeringii tissue culture culture medium |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618919A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | A kind of cultural method improving exhibition shape cherry iris flowering rate |
| CN110999792A (en) * | 2019-12-30 | 2020-04-14 | 临沧市云瑞堂生物科技有限公司 | Multi-bud culture method for anoectochilus roxburghii |
| CN113229147A (en) * | 2021-06-07 | 2021-08-10 | 芜湖东源新农村开发股份有限公司 | Method for cultivating differentiated bud of butterfly orchid seedling by using extreme illumination |
| CN115104535A (en) * | 2022-07-20 | 2022-09-27 | 浙江农林大学 | Method for regenerating phalaenopsis into complete plant by taking leaves as explants |
| CN115104535B (en) * | 2022-07-20 | 2023-09-19 | 浙江农林大学 | Method for regenerating butterfly orchid into complete plant by taking leaf as explant |
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| CN103766218B (en) | 2015-05-13 |
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