CN113208014A - Feed with high spawning proliferation rate of natural enemy ladybug and application - Google Patents
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- 230000035755 proliferation Effects 0.000 title claims description 6
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- 238000012136 culture method Methods 0.000 claims abstract description 9
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 42
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- 229930006000 Sucrose Natural products 0.000 claims description 27
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 25
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- 235000012907 honey Nutrition 0.000 claims description 22
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- 229930091371 Fructose Natural products 0.000 claims description 18
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 18
- 239000007864 aqueous solution Substances 0.000 claims description 13
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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Abstract
The invention discloses a feed with high spawning multiplication rate of natural enemy ladybug and application thereof, belonging to the technical field of insect culture. The feed comprises: sugar sources and fruit fly larvae, and discloses a culture method for the natural enemy ladybug high-yield egg multiplication rate.
Description
Technical Field
The invention belongs to the technical field of insect culture, and particularly relates to a feed with high spawning multiplication rate of natural enemy ladybug and application thereof.
Background
Biological control plays a very important role in the integrated control of pests. With the recent emphasis on biological control, natural enemy insects have been increasingly used for the comprehensive control of pests. The harmonia axyridis can prey on various pests such as aphids, whiteflies, mites and the like, is one of very important predatory natural enemy insects, and is hopefully applied to biological control of agricultural and forestry pests in a large quantity. Therefore, the artificial breeding and propagation of the harmonia axyridis have great economic and social values, and the significance of exploring the large-scale breeding technology of the harmonia axyridis is great. In recent years, although people optimize artificial feeding technology and feed formula of harmonia axyridis for several times, various problems still exist, such as:
1. long breeding period, large risk, high cost, low breeding rate and the like;
2. when some living feeds are singly used for feeding, the harmonia axyridis has the problems of low larva survival rate, reduced reproductive capacity, poor predation capacity and the like.
Therefore, the research on the artificial breeding technology of the harmonia axyridis generally focuses on the discovery of artificial feed formulas or living feeds at present, and the research on methods for improving the egg laying proliferation of the harmonia axyridis by using the artificial breeding technology and the living feeds is not many.
Therefore, the problem to be solved in the field is how to provide the feed for breeding the natural enemy ladybug with high egg laying rate and the application thereof.
Disclosure of Invention
The invention discloses a feed with high spawning multiplication rate of natural enemy ladybug and application thereof. The study proves that the sugar source supplement can improve the service life, the reproductive capacity and the predation capacity of the harmonia axyridis. Therefore, the sugar source feed is taken as a supplementary nutrient source, and the defects of the living feed in the breeding of the harmonia axyridis are improved.
In order to achieve the purpose, the invention adopts the following technical scheme:
a combined feed for natural enemy ladybug high-yield egg proliferation rate comprises: sugar sources and Drosophila larvae;
preferably, the sugar source comprises at least: one of sucrose, honey or fructose;
preferably, the sucrose, honey and fructose are in a solution state;
preferably, the sucrose, honey and fructose are aqueous solutions;
preferably, the mass fraction of solute in the sucrose, honey or fructose aqueous solution is 5-15%;
preferably, in the water-soluble sucrose, honey or fructose, the mass fraction of solute is 10%;
preferably, the method is characterized by comprising the following steps:
(1) the ladybug adult is treated according to the following steps: every day, 10-15 fruit fly larvae per adult are provided, and the adult is cultured in a specification of 5-15% of sugar source water solution until the adult lays eggs.
(2) Hatching eggs after ladybug spawning to obtain ladybug larvae;
(3) pressing ladybug larvae into 1-2 instar larvae: providing 3-5 fruit fly larvae per larva per day, and culturing with 10% sugar source water solution; larvae of 3-4 instars: providing 10-15 fruit fly larvae per larva per day, and culturing with 10% sugar source water solution; feeding to adult;
preferably, the weight fraction of the sugar source aqueous solution is 10%;
preferably, in the step (3), feeding is carried out through the water-absorbing carrier, the sugar source water solution is added once every 2 days, and the water-absorbing substance is replaced once every 10 days;
preferably, the water absorption carrier is absorbent cotton;
preferably, in the step (1) and the step (3), white toilet paper is placed as an egg laying medium;
(4) carrying out amplification culture or producing worm eggs according to the culture method in the step (1);
preferably, in the step (1), the drosophila larvae are aged larvae which are hatched for 3-4 days;
preferably, in the step (1), the sugar source aqueous solution at least comprises: one of sucrose aqueous solution, honey aqueous solution or fructose aqueous solution;
preferably, in the step (1), the feeding method of 10% sugar source water is as follows: feeding with water-absorbing carrier, adding sugar water solution every 2 days, and replacing water-absorbing carrier every 10 days;
preferably, the water absorption carrier is absorbent cotton;
preferably, in the step (2), the hatching conditions of the worm eggs are as follows: 26 +/-1 ℃, relative humidity of 75 +/-5%, 16L: and (5) culturing under 8D illumination conditions.
The 16L: 8D is 16h light and 8h dark conditions;
in conclusion, the invention discloses a feed with high spawning multiplication rate for natural enemy ladybug and application thereof, and solves the problems of low reproductive capacity, low larva survival rate, low predation capacity and the like of the ladybug under the condition of a single living body breeding source.
Firstly, sugar source feed is supplemented in feeding, and the problem that ladybug does not lay eggs or less lays eggs can be solved. Laboratory studies have shown that: the harmonia axyridis raised only with drosophila larvae did not lay eggs. The average egg laying amount per day of 1 pair of harmonia axyridis is about 17.8 grains under the condition of adding 10% of honey water, and the average egg laying amount per day is about 21.2 grains under the condition of adding 10% of sucrose water. At 26 ± 1 ℃ and RH 75 ± 5%, 16L: under the condition of 8D illumination, the hatching rate of honey treated eggs is 87.89%, and the hatching rate of sucrose treated eggs is 90.47%. Laboratory studies prove that the coccinella variegate laying amount is larger and the egg hatching rate is higher under the treatment of 10% of sucrose.
And secondly, increasing the survival rate of the larvae of the harmonia axyridis. Laboratory studies show that the survival rate of the harmonia axyridis bred by only using the drosophila larvae is 76.66 percent; the survival rate of the honey and the drosophila larvae is 80 percent; the survival rate of the cane sugar and the fruit fly larvae and the survival rate of the fructose and the fruit fly larvae are 93.33 percent. The survival rate of the larvae treated by the honey water is low, and the survival rate of the larvae treated by the cane sugar water is high.
And thirdly, the weight of 4-instar larvae and adults is improved. The study shows that the weight of 4-instar larvae bred by clear water control is 0.0263g, and the weight of primary eclosion imagoes is 0.0178 g; honey is added to feed 4-instar larvae with the weight of 0.0290g and primary eclosion imagoes with the weight of 0.0206 g; adding fructose to feed 4-instar larva with weight of 0.0311 and adult weight of 0.0222; the weight of the 4 th larva is 0.0313g, and the weight of the primary eclosion imago is 0.0227 g. The addition of sucrose water treated 4 instar larvae and adults gained significantly.
And fourthly, the 4 th instar larvae and adults obtained in a sugar water feeding environment have larger capturing amount and better predation capacity. Research proves that the average daily predation amount of 4-instar larvae is about 0.0105g and the average daily predation amount of adults is about 0.0114g under the control of clear water; the average daily predation amount of 4-instar larvae fed with fructose is about 0.0118g, and the average daily predation amount of adults is about 0.0134 g; the average daily predation amount of 4-instar larvae fed with sucrose is about 0.0126g, and the average daily predation amount of adults is about 0.0134 g; the average daily predation amount of 4-instar larvae fed with honey is about 0.0132g, and the average daily predation amount of adults is about 0.0160 g. The predation amount of the adult insects treated by adding the sugar-source feed is obviously increased.
The combination shows that: the method for breeding the harmonia axyridis by using the drosophila melanogaster larvae and 10% of cane sugar water feed enables the harmonia axyridis to grow and propagate normally, increases the egg laying amount and the survival rate of the adult harmonia axyridis, shortens the development period of the larvae, improves the weight of the 4 th larvae and the adult harmonia axyridis, and improves the predation capacity of the harmonia axyridis.
Drawings
FIG. 1 is a process flow chart of a culture method for natural enemy ladybug with high spawning multiplication rate.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Obtaining drosophila larvae:
(1) preparation of a culture medium: the main components comprise the following components in parts by weight: 380 parts of water, 135 parts of sugar, 15 parts of agar, 100 parts of corn flour and 7 parts of yeast powder; 5 parts of propionic acid; the preparation method comprises the following steps: dividing horizontally into two parts, adding agar into one part, boiling, stirring to melt, adding sucrose, adding corn flour into the other part, stirring to paste, adding agar water, adding propionic acid, stirring to paste, keeping boiling for 3 min to obtain homogeneous culture medium, stopping heating, and slowly cooling: adding yeast powder before the culture medium is cooled and not solidified, stirring, subpackaging in sterilized conical flasks, and cooling and solidifying the culture medium for later use.
(2) Obtaining drosophila larvae: opening the mouth of the culture medium bottle for later use, airing, adding 10-15 pairs of seed flies into the bottle, covering the bottle with a bottle cap, and placing the bottle at the temperature of 28 +/-1 ℃ and the RH of 75 +/-5 percent and 16L: culturing for 24 hours under 8D illumination, laying eggs in a culture medium after female flies mate, taking out seed flies after laying eggs, putting the seed flies into another culture bottle, and incubating the culture medium with the eggs in an insect breeding chamber for about 24 hours to obtain larvae; breeding for 2-3 days after the larvae are hatched, adding distilled water into a culture bottle to 2/3, allowing the larvae to climb out of the culture medium and enter a water body, and filtering by using a screen to separate the larvae; adding yeast powder into the culture medium from which the larvae are separated, continuously inoculating the drosophila larvae, continuously using for three times, adding water into the culture medium, stirring, placing the culture medium in a plastic basin, air-drying under natural conditions, taking out the unseparated drosophila larvae which are hidden in the culture medium after the drosophila larvae are air-dried by using tweezers, and discarding the air-dried culture medium. 20 fruit flies are added into each bottle of the culture medium prepared by the method to lay eggs for one day and then are taken out, 200 and 300 mature fruit fly larvae can be obtained after the culture of the culture medium is carried out for 3-4 days, the yield is huge, the culture medium prepared by the method can be recycled, the cost and the waste of materials are reduced, and the mature larvae incubated for 3-4 days are selected to be fed, and the same is carried out below.
Example 1 Breeding of harmonia axyridis with Drosophila larvae + sucrose solution
(1) 1 pair of harmonia axyridis was placed in 8 x 8cm plastic boxes covered with gauze, and absorbent cotton dipped with 10% sucrose solution and white toilet paper were placed therein for oviposition and fed with 10-15 drosophila larvae per day. Feeding once every 1 day, adding sugar water once every 2 days, and replacing absorbent cotton once every 10 days. Egg masses were collected every 2 days. Egg 106 particles were collected for 5 days.
(2) Taking out white toilet paper with ovum, and placing the toilet paper at 26 +/-1 ℃ and RH of 75 +/-5%, 16L: culturing under 8D illumination condition until hatching to obtain 95 harmonia axyridis larvae.
(3) 30 hatched larvae were individually placed in a 29mm by 104mm plastic bottle, a hole was punched in the plastic bottle cap, and absorbent cotton dipped with 10% sucrose solution was added thereto. Feeding 1-2 instar larvae with 3-5 fruit fly larvae per day, feeding 3-4 instar larvae with 10-15 fruit fly larvae per day, feeding once every 1 day, adding sugar water once every 2 days by using a plastic dropper, changing absorbent cotton once every 10 days, and feeding to adults. To obtain 28 heads of adults. The average calendar period of the larva is 13.5 +/-1.1 d, the average weight of 4-instar larva is 0.0313g, and the average weight of the primary eclosion imago is 0.0227 g. The average daily predation of 4 instar larvae is about 0.0126g, and the average daily predation of adults is about 0.0134 g.
Example 2 Breeding of harmonia axyridis with Drosophila larvae + Honey solution
(1) 1 pair of harmonia axyridis was placed in 8 x 8cm plastic boxes covered with gauze, and absorbent cotton dipped with 10% honey water and white toilet paper were placed therein for oviposition and fed with 10-15 heads of drosophila larvae per day. Feeding once every 1 day, adding sugar water once every 2 days, and replacing absorbent cotton once every 10 days. Egg masses were collected every 2 days. 89 eggs were collected for 5 days.
(2) Taking out white toilet paper with ovum, and placing the toilet paper at 26 +/-1 ℃ and RH of 75 +/-5%, 16L: culturing under 8D illumination condition until hatching to obtain 77 harmonia axyridis larvae.
(3) Placing 30 hatched larvae into 29mm by 104mm plastic bottles, pricking holes on the plastic bottle covers, adding absorbent cotton dipped with 10% honey water into the bottles, feeding the larvae of 1-2 years with 3-5 drosophila larvae per day and the larvae of 3-4 years with 10-15 drosophila larvae per day, feeding once every 1 day, adding sugar water once every 2 days by using a plastic dropper, replacing the absorbent cotton once every 10 days, and feeding the larvae to adults. To obtain 24 adult worms. The average duration of the larvae is 13.3 +/-1.2 d. The average weight of 4-instar larvae is 0.0290g, and the average weight of primary eclosion imagoes is 0.0206 g. The average daily predation of 4 instar larvae is about 0.0132g, and the average daily predation of adults is about 0.0160 g.
Example 3 Breeding of harmonia axyridis with Drosophila larvae + fructose solution
(1) 1 pair of harmonia axyridis was placed in 8 x 8cm plastic boxes covered with gauze, and absorbent cotton dipped with 10% fructose solution and white toilet paper were placed therein for oviposition and fed per 10-15 drosophila larvae per day. Feeding once every 1 day, adding sugar water once every 2 days, and replacing absorbent cotton once every 10 days. Egg masses were collected every 2 days. Egg particles 67 were collected for 5 days.
(2) Taking out white toilet paper with ovum, and placing the toilet paper at 26 +/-1 ℃ and RH of 75 +/-5%, 16L: and culturing under 8D illumination condition until hatching to obtain 59 harmonia axyridis larvae.
(3) Placing 30 hatched larvae into 29mm by 104mm plastic bottles, pricking holes on the plastic bottle covers, adding absorbent cotton dipped with 10% fructose solution, feeding 1-2 instar larvae with 3-5 drosophila larvae per day and 3-4 instar larvae with 10-15 drosophila larvae per day, feeding once every 1 day, adding sugar solution once every 2 days, changing absorbent cotton every 10 days, and feeding to adults. To obtain 28 heads of adults. The average duration of the larvae is 14.0 +/-1.4 d. The average weight of 4-instar larvae is 0.0311g, and the average weight of primary eclosion imagoes is 0.0131 g. The average daily predation of 4 instar larvae is about 0.0118g, and the average daily predation of adults is about 0.0134 g.
Comparative example 1 independent rearing of Drosophila melanogaster larvae
(1) 1 pair of harmonia axyridis was placed in 8 x 8cm plastic boxes covered with gauze, and absorbent cotton dipped with clear water and white toilet paper were placed therein for oviposition, and fed with 10-15 heads of drosophila larvae per day. Feeding once every 1 day, adding clear water once every 2 days by using a plastic dropper, and replacing absorbent cotton once every 10 days. Egg masses were collected every 2 days. No egg grains were collected for 5 days, and no egg grains were found after continuous feeding for 1 month.
(2) Eggs from harmonia axyridis bred using sucrose + drosophila larvae were placed at 26 ± 1 ℃ with RH 75 ± 5%, 16L: and (5) culturing under 8D illumination condition until hatching. 30 larvae are taken, each larva is independently raised in a plastic bottle with the diameter of 29mm x 104mm, holes are formed in a plastic bottle cover, absorbent cotton dipped with clear water is added into the plastic bottle, 3-5 drosophila larvae per larva per day of 1-2 instar larvae and 10-15 drosophila larvae per larva per day of 2-4 instar larvae are fed, clear water is added once every 2 days by using a plastic dropper, the absorbent cotton is replaced once every 10 days, and the adult ladybug is fed to obtain 22 harmonia axyridis. The average duration of the larvae is 13.8 +/-1.4 d. The weight of 4-instar larva is 0.0263g, and the weight of primary eclosion imago is 0.0178 g. The average daily predation amount of 4-instar larvae is about 0.0105g, and the average daily predation amount of adults is about 0.0114 g.
Comparative example 2 independent rearing of harmonia axyridis in syrup
(1) 1 pair of harmonia axyridis is placed in a plastic box of 8 x 8cm covered by gauze, absorbent cotton dipped with 10% sucrose solution and white toilet paper are placed in the box for spawning, the sugar solution is added once every 2 days by using a plastic dropper, and the absorbent cotton is replaced once every 10 days. Egg masses were collected every 2 days. No egg grains are collected within 5 days, no egg grains are found after continuous breeding for 1 month, and the harmonia axyridis is dead after 21 days.
(2) 1 pair of harmonia axyridis is placed in a plastic box 8 x 8cm covered with gauze, absorbent cotton dipped with 10% honey water and white toilet paper are placed therein for oviposition, sugar water is added every 2 days, and absorbent cotton is replaced every 10 days. Egg masses were collected every 2 days. No egg grains are collected within 5 days, no egg grains are found in continuous feeding, and the phenomenon of death of the harmonia axyridis occurs after 15 days.
(3) Placing 1 pair of harmonia axyridis in 8 x 8cm plastic box covered with gauze, placing absorbent cotton dipped with 10% fructose solution and white toilet paper therein for oviposition, adding sugar solution once every 2 days, and replacing absorbent cotton once every 10 days. Egg masses were collected every 2 days. No egg grains are collected within 5 days, no egg grains are found in continuous feeding, and the harmonia axyridis is dead after 13 days.
(4) Since no eggs were obtained in steps (1) (2) (3), the eggs produced by harmonia axyridis of example 1 (rearing of sucrose + drosophila larvae) were placed at a temperature of 26 ± 1 ℃ with RH of 75 ± 5%, 16L: and (5) culturing under 8D illumination condition until hatching. 30 larvae are taken, each larva is independently bred in a plastic bottle with the diameter of 29mm by 104mm, holes are formed in a plastic bottle cover, absorbent cotton dipped with 10% sucrose solution is added into the plastic bottle, 73.3% of the harmonia axyridis is killed after entering the second instar, the rest harmonia axyridis is killed at the 1 instar, and 0 adult beetle is obtained.
The result shows that the ladybug which only uses the drosophila larvae or the sugar source feed does not lay eggs, and the problem that the ladybug does not lay eggs or has less eggs can be solved by using the sugar source feed and the drosophila larvae to jointly feed the ladybug. Although there has been previous experience in raising harmonia axyridis using Drosophila larvae + honey substrate, it was limited to adult stage nutrient supplementation. Laboratory studies have demonstrated that: the breeding of the harmonia axyridis larvae by using the sugar source feed and the drosophila larvae can obviously improve the survival rate and the predation capacity of the harmonia axyridis larvae, the obtained adult quantity is obviously higher than that of the clear water and the drosophila larvae, the weight of the 4-instar larvae and the adult of the harmonia axyridis is improved, and the breeding rate of the harmonia axyridis is improved; the obtained 4 th instar larva and imago have larger capturing amount and better capturing capability in a sweet water feeding environment.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to the above-described embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. The combined feed for the natural enemy ladybug high-yield egg proliferation rate is characterized by comprising the following components: sugar sources and drosophila larvae.
2. The combination feed of claim 1, wherein the sugar source comprises at least: sucrose, honey or fructose.
3. The combination feed of claim 2, wherein the sucrose, honey and fructose are in solution.
4. A combined feed as claimed in claim 3, characterised in that the sucrose, honey and fructose are in aqueous solution.
5. The combined feed of claim 4, wherein the aqueous solution of sucrose, honey or fructose has a solute content of 5-15% by weight.
6. A culture method for natural enemy ladybug high-yield egg proliferation rate is characterized by comprising the following steps:
(1) the ladybug adult is treated according to the following steps: every day, 10-15 fruit fly larvae per adult are provided, and the adult is cultured in a specification of 5-15% of sugar source water solution until the adult lays eggs.
(2) Hatching eggs after ladybug spawning to obtain ladybug larvae;
(3) pressing ladybug larvae into 1-2 instar larvae: providing 3-5 fruit fly larvae per larva per day, and culturing with 10% sugar source water solution; larvae of 3-4 instars: providing 10-15 fruit fly larvae per larva per day, and culturing with 10% sugar source water solution; feeding to adult;
(4) carrying out amplification culture or producing worm eggs according to the culture method in the step (1).
7. The culture method according to claim 6, wherein in the step (1), the Drosophila larvae are aged larvae which have hatched for 3 to 4 days.
8. The culture method according to claim 6, wherein in the step (1), the sugar-source aqueous solution comprises at least: one of sucrose aqueous solution, honey aqueous solution or fructose aqueous solution.
9. The culture method according to claim 6, wherein in the step (1), the 10% sugar source is fed in water by: feeding with water-absorbing carrier, adding sugar water solution every 2 days, and replacing water-absorbing carrier every 10 days.
10. The culture method according to claim 6, wherein in the step (2), the hatching conditions of the eggs are: 26 +/-1 ℃, relative humidity of 75 +/-5%, 16L: and (5) culturing under 8D illumination conditions.
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| CN101940271A (en) * | 2010-09-27 | 2011-01-12 | 中国农业科学院植物保护研究所 | Artificial feed for natural enemy insects |
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| CN108575912A (en) * | 2018-03-13 | 2018-09-28 | 中国农业科学院棉花研究所 | A kind of method that evaluation insecticidal proteins influence Propylaea japonica Adults and feeding device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940271A (en) * | 2010-09-27 | 2011-01-12 | 中国农业科学院植物保护研究所 | Artificial feed for natural enemy insects |
| CN104770339A (en) * | 2015-03-27 | 2015-07-15 | 中国热带农业科学院热带生物技术研究所 | Method for artificially breeding alesia discolor fabricius |
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