CN113151025A - 一种融合表达β-甘露聚糖酶和α-半乳糖苷酶的毕赤酵母、制备方法及其应用 - Google Patents
一种融合表达β-甘露聚糖酶和α-半乳糖苷酶的毕赤酵母、制备方法及其应用 Download PDFInfo
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- CN113151025A CN113151025A CN202110270368.XA CN202110270368A CN113151025A CN 113151025 A CN113151025 A CN 113151025A CN 202110270368 A CN202110270368 A CN 202110270368A CN 113151025 A CN113151025 A CN 113151025A
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- alpha
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- galactosidase
- pichia pastoris
- mannase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/2488—Mannanases
- C12N9/2494—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01022—Alpha-galactosidase (3.2.1.22)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01078—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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Abstract
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Priority Applications (1)
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CN202110270368.XA CN113151025A (zh) | 2021-03-12 | 2021-03-12 | 一种融合表达β-甘露聚糖酶和α-半乳糖苷酶的毕赤酵母、制备方法及其应用 |
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CN202110270368.XA CN113151025A (zh) | 2021-03-12 | 2021-03-12 | 一种融合表达β-甘露聚糖酶和α-半乳糖苷酶的毕赤酵母、制备方法及其应用 |
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CN113151025A true CN113151025A (zh) | 2021-07-23 |
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CN202110270368.XA Pending CN113151025A (zh) | 2021-03-12 | 2021-03-12 | 一种融合表达β-甘露聚糖酶和α-半乳糖苷酶的毕赤酵母、制备方法及其应用 |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0255153A1 (en) * | 1986-06-03 | 1988-02-03 | Unilever N.V. | Production of guar alpha-galactosidase by hosts transformed by recombinant DNA methods |
CN102559526A (zh) * | 2011-12-12 | 2012-07-11 | 江南大学 | 一种共表达An Man5A和XynⅡ的毕赤酵母系统 |
US20160097053A1 (en) * | 2013-03-08 | 2016-04-07 | Keck Graduate Institute Of Applied Life Sciences | Yeast promoters from pichia pastoris |
CN110863005A (zh) * | 2019-12-30 | 2020-03-06 | 河南省科学院生物研究所有限责任公司 | 一种通过共表达血红蛋白来提高毕赤酵母工程菌中α-半乳糖苷酶组成型表达量的方法 |
CN112391425A (zh) * | 2020-10-22 | 2021-02-23 | 天津科技大学 | 一种协同水解槐豆胶的方法 |
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2021
- 2021-03-12 CN CN202110270368.XA patent/CN113151025A/zh active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0255153A1 (en) * | 1986-06-03 | 1988-02-03 | Unilever N.V. | Production of guar alpha-galactosidase by hosts transformed by recombinant DNA methods |
CN102559526A (zh) * | 2011-12-12 | 2012-07-11 | 江南大学 | 一种共表达An Man5A和XynⅡ的毕赤酵母系统 |
US20160097053A1 (en) * | 2013-03-08 | 2016-04-07 | Keck Graduate Institute Of Applied Life Sciences | Yeast promoters from pichia pastoris |
CN110863005A (zh) * | 2019-12-30 | 2020-03-06 | 河南省科学院生物研究所有限责任公司 | 一种通过共表达血红蛋白来提高毕赤酵母工程菌中α-半乳糖苷酶组成型表达量的方法 |
CN112391425A (zh) * | 2020-10-22 | 2021-02-23 | 天津科技大学 | 一种协同水解槐豆胶的方法 |
Non-Patent Citations (6)
Title |
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GUO N等: "Engineered bifunctional enzymes of endo-1,4-beta-xylanase/endo-1,4-beta-mannanase were constructed for synergistically hydrolyzing hemicellulose", 《JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC》, vol. 97, pages 311 - 318 * |
MALHERBE AR等: "Expression and evaluation of enzymes required for the hydrolysis of galactomannan", 《J IND MICROBIOL BIOTECHNOL》, vol. 41, no. 08, pages 1201 - 1209 * |
姚银等: "木聚糖酶和甘露聚糖酶在毕赤酵母中的共表达及产酶分析", 《中国生物工程杂志》, vol. 39, no. 03, pages 37 - 45 * |
孔海洋等: "β-甘露聚糖酶Man5A和木聚糖酶Tlxyn11B的融合表达", 《生物工程学报》, vol. 36, no. 09, pages 1849 - 1858 * |
张献伟等: "木聚糖酶-甘露聚糖酶融合酶基因Linker优化及其在猪肾pK15细胞中共表达", 《中国农业科学》, vol. 46, no. 22, pages 4774 - 4783 * |
黄生平等: "植酸酶和甘露聚糖酶双功能毕赤酵母工程菌的构建和产酶分析", 《微生物学报》, vol. 47, no. 02, pages 280 - 284 * |
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Inventor after: Zhu Youmin Inventor after: Zhong Cheng Inventor after: Yu Tingting Inventor after: Zhang Hui Inventor before: Zhu Youmin Inventor before: Zhong Cheng Inventor before: Yu Tingting Inventor before: Zhang Hui Inventor before: Zheng Yehong Inventor before: Lei Yu Inventor before: Meng Weilong Inventor before: Zhang Wenhui Inventor before: Lin Lili |