CN113143810A

CN113143810A - Preparation method of soluble mulberry leaf spot-fading mask

Info

Publication number: CN113143810A
Application number: CN202011016063.8A
Authority: CN
Inventors: 翁朝阳
Original assignee: Fujian Tuoxi New Material Technology Co ltd
Current assignee: Fujian Tuoxi New Material Technology Co ltd
Priority date: 2020-09-24
Filing date: 2020-09-24
Publication date: 2021-07-23

Abstract

The invention relates to a cosmetic, in particular to a preparation method of a soluble mulberry leaf spot-fading mask, which comprises the steps of taking deionized water, sequentially adding a tremella fruiting body extract, sodium phytate, 1, 3-propylene glycol, allantoin, 1, 2-pentanediol and isoprene glycol, stirring and mixing until the mixture is transparent and uniform; cooling to 50 deg.C, adding folium Mori extract and herba Portulacae extract, and stirring; cooling to 45 deg.C, adding glycerol and anisic acid phase, cooling to 40 deg.C, and stopping stirring to obtain facial mask solution; mixing deacetylated chitin, xylitol and distilled water to prepare substrate membrane liquid; and mixing and stirring the mask solution and the substrate solution uniformly, pouring the mixture into a mold, and drying the mixture by hot air at 40-55 ℃ until the mask is formed. The facial mask liquid prepared by the method has strong melanin resistance, and the preparation method is simple and can reduce the cost. The mask disclosed by the invention is strong in solubility and high in safety, does not need to be added with a preservative, and does not cause waste.

Description

Preparation method of soluble mulberry leaf spot-fading mask

Technical Field

The invention relates to a cosmetic, and in particular relates to a mulberry leaf spot-fading mask liquid and a preparation method thereof.

Background

Under the irradiation of ultraviolet rays, keratinocytes in the skin produce endothelin, which binds to melanocyte receptors, and melanocytes receive an instruction to synthesize melanin, and begin to synthesize melanin. The synthesis process is shown in figure 3.

Free radicals produced by the skin participate in the reaction process of tyrosinase; the free radicals can also react with fatty acid to generate malondialdehyde, and react with protein on cell membrane to form lipofuscin, which is deposited on skin to form various color spots.

Folium mori and mulberry branches have long been used for whitening, and people drink decoction of mulberry branches and leaves and mash fresh folium mori and apply the fresh folium mori to faces to treat color spots and whelks. Modern researches have proved that the extracts of folium Mori, ramulus Mori and cortex Mori can effectively reduce melanin generation by inhibiting tyrosinase activity. But the content of effective substances of mulberry leaves in the mulberry leaf extract is low, so that the effect of the facial mask liquid is reduced. Or, in order to improve the content of effective substances of mulberry leaf extract species, the purification process is complex, the cost is increased, and the price of the facial mask liquid is overhigh.

In addition, facial masks in the market at present mostly adopt high-adsorbability mask cloth to load mask liquid, and due to the high adsorbability of the mask cloth, too much mask liquid is remained on the mask cloth after the mask cloth is used, so that waste is caused, and the mask cloth can cause the facial masks to be dry and absorb moisture on the skin reversely if the mask cloth is applied on the face for a long time.

Disclosure of Invention

Technical problem to be solved

In order to solve the above problems in the prior art, the invention provides a preparation method of a soluble mulberry leaf spot-lightening mask for improving the melanin removing efficiency of a mulberry leaf extract.

(II) technical scheme

In order to achieve the purpose, the invention adopts the main technical scheme that:

a preparation method of a soluble mulberry leaf spot-fading mask comprises the following steps:

s1 preparation of facial mask liquid:

s11 preparation of mulberry leaf extract: the mulberry leaf extract is mainly extracted from fresh mulberry leaves, mulberry twigs and mulberry bark;

preparation of S12 purslane extract: extracting herba Portulacae with alcohol to obtain herba Portulacae extract;

s13 mixing the folium Mori extract and herba Portulacae extract to obtain phase B; mixing water, glycerol and anisic acid into phase C in advance;

s14 heating deionized water to 75-80 deg.C, sequentially adding Tremella fruiting body extract, sodium phytate, 1, 3-propylene glycol, allantoin, 1, 2-pentanediol and isoprene glycol, stirring and mixing to transparent and uniform; cooling to 50 ℃, adding the phase B and stirring uniformly; continuously cooling to 45 ℃, adding the phase C, uniformly stirring, cooling to 40 ℃, and stopping stirring to obtain the mask liquid;

preparing S2 base membrane liquid: mixing deacetylated chitin, xylitol and distilled water according to the weight part ratio of 1: 100 to prepare substrate membrane liquid;

s3 mixing: mixing and stirring the obtained mask liquid and the substrate film liquid uniformly according to the volume ratio of 1: 10-20, and pouring the mixture into a mold to ensure that the thickness of the liquid is 1-3 mm;

s4 forming: and (4) drying the mould with the poured liquid by hot air at 40-55 ℃ until the mask is formed.

Further, in step S11, the preparation of the mulberry leaf extract comprises the following steps:

grinding fresh and cleaned mulberry leaves into pulp, adding 10% rice pulp in the same weight part, continuously stirring and uniformly stirring, adding 20-30% of 1, 3-propylene glycol in weight of the mulberry leaves, heating in a water bath at 35 ℃ for 25-35 min, heating in a water bath at 95 ℃ for 15-20 min, adding 20-30% of mulberry leaves, continuously stirring for 15min, centrifuging to remove residues to obtain mulberry leaf liquid, and filtering the obtained mulberry leaf liquid through a 0.35 mu m filter membrane to obtain a mulberry leaf extracting solution;

respectively grinding fresh and cleaned ramulus mori and cortex mori into powder, mixing the powder into composite powder, adding deionized water with the same weight part of the composite powder, adding neutral protease according to the addition amount of 25000u/g of the composite powder for enzymolysis, heating in a water bath for 5min at 100 ℃ for enzyme deactivation, extracting with ethanol, and filtering with a 0.35 mu m filter membrane to obtain a composite extracting solution;

and uniformly mixing the obtained mulberry leaf extracting solution and the composite extracting solution with the same volume part to obtain the mulberry leaf extract.

Further, in step S12, the preparation of the purslane extract includes the following steps: grinding fresh and clean purslane into slurry, adding 30-90% of ethanol, soaking for 1 hour at the temperature of 35 ℃ and under the pressure of 0.4MPa, adding 30-90% of ethanol, refluxing and extracting for 3 times, 1 hour each time, and concentrating by a thin film evaporation method to obtain the purslane extract.

Further, in step S14, the preparation of the tremella fruiting body extract includes the following steps: cleaning and crushing fresh tremella sporophore, adding distilled water in an amount which is 20-30 times the weight of the fresh tremella sporophore, heating the mixture for 20-30 min under 100Mpa, and filtering to remove impurities to obtain the tremella sporophore extract.

Further, the mask liquid obtained in the step S14 comprises the following components in percentage by weight: 0.1-0.3% of tremella fruiting body extract, 0.03-0.06% of sodium phytate, 6-9% of 1, 3-propylene glycol, 0.03-0.15% of allantoin, 2-4% of 1, 2-pentanediol, 1-3% of isoprene glycol, 1-3% of glycerol, 1-3% of anisic acid, 1-3% of sodium hydroxide, 3-6% of mulberry leaf extract, 0.5-2% of purslane extract and the balance of deionized water.

Further, the mask liquid obtained in the step S14 comprises the following components in percentage by weight: 0.2% of tremella fruiting body extract, 0.05% of sodium phytate, 7% of 1, 3-propylene glycol, 0.1% of allantoin, 3% of 1, 2-pentanediol, 2% of isoprene glycol, 1% of glycerol, 1% of anisic acid, 1% of sodium hydroxide, 5% of mulberry leaf extract, 1% of purslane extract and the balance of deionized water.

(III) advantageous effects

The invention has the beneficial effects that:

1. the facial mask liquid in the facial mask prepared by the method has stronger melanin resistance, and the preparation method is simple and can reduce the cost.

2. The facial mask obtained by the method disclosed by the invention is strong in solubility and high in safety, and does not need to be added with a preservative, so that waste is avoided.

Drawings

FIG. 1 shows the UV absorption ability of mulberry leaf extracts of different concentrations;

FIG. 2 shows the inhibition rate of tyrosinase by mulberry leaf extract with different concentrations;

FIG. 3 is a schematic diagram of the synthesis of melanin in the skin.

Detailed Description

For a better understanding of the present invention, reference will now be made in detail to the present invention by way of specific embodiments thereof.

s1 preparation of facial mask liquid:

The invention extracts the flavonoid substance from the mulberry leaves by rice milk, and mixes the extract of the mulberry twigs and the mulberry bark with the extract of the mulberry leaves, thereby further improving the anti-melanin capability of the mulberry leaf extract. In addition, in step S12, more antioxidant small-molecule peptide substances are obtained by enzymolysis and extraction with neutral protease, and enter the skin surface basement together with other melanin-inhibiting substances (such as flavonoids) to reduce free radicals, thereby reducing the production rate of melanin substances.

When the facial mask is used, the face is wetted after the face is cleaned, the facial mask is attached to the face, and water or essence is continuously beaten on the facial mask; or spraying water on the facial mask until the facial mask is absorbed by face

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

Example 1

A preparation method of folium Mori extract comprises the following steps:

s1 preparation of mulberry leaf extract: grinding fresh and cleaned mulberry leaves into pulp, adding 10% rice pulp in the same weight part, continuously stirring and uniformly stirring, adding 25% of 1, 3-propylene glycol in weight of the mulberry leaves, heating in a water bath at 35 ℃ for 30min, heating in a water bath at 95 ℃ for 18min, adding 28% of mulberry leaves, continuously stirring for 15min, centrifuging to remove residues to obtain mulberry leaf liquid, and filtering the obtained mulberry leaf liquid through a 0.35 mu m filter membrane to obtain a mulberry leaf extracting solution;

s2 preparation of the compound extract of ramulus mori and cortex mori: respectively grinding fresh and cleaned ramulus mori and cortex mori into powder, mixing the powder according to the weight ratio of 1: 1 to form composite powder, adding deionized water with the same weight part of the composite powder, adding neutral protease according to the addition amount of 25000u/g of the composite powder, performing enzymolysis for 25min at 35 ℃, performing water bath heating for 5min at 100 ℃ to inactivate enzyme, performing alcohol extraction by using ethanol with the mass concentration of 75% and 3 times of the weight of the composite powder, heating for 30min at 35 ℃, and filtering by using a 0.35-micrometer filter membrane to obtain a composite extracting solution;

s3, mixing the mulberry leaf extract and the compound extract with the same volume part uniformly to obtain the mulberry leaf extract.

The mulberry leaf extract prepared by the embodiment has strong ultraviolet absorption capacity, can inhibit over 90% of tyrosinase activity, and has strong capacity of scavenging free radicals.

The extract of example 1 was lyophilized and then prepared into solutions of different concentrations, and the ability to absorb ultraviolet rays was tested separately, as shown in fig. 1. The ultraviolet ray absorbing capacity can be very strong at a mass concentration of 0.05%, and the absorbing capacity is increased with the increase of the concentration.

After the extract of example 1 was freeze-dried, the same amount was weighed, and diluted with 50% by mass aqueous alcohol solution to obtain three groups of 5% sample solutions, one group was treated with 4500lx of strong light for 10 days, one group was treated at 60 ℃ for 10 days, and the other group was not treated at room temperature for 10 days, and the three groups of sample solutions were allowed to absorb ultraviolet ray, and the data shown in fig. 2 were obtained. Therefore, the mulberry leaf extract obtained by the invention has good stability, and still has excellent ultraviolet absorption effect after strong light and high temperature action.

The extract of the embodiment 1 is freeze-dried to obtain mulberry leaf freeze-dried powder, the mulberry leaf freeze-dried powder and deionized water are prepared into different solution concentrations, the solution with each concentration and tyrosinase are added into a tyrosinase solution together for reaction, then the absorbance value at the wavelength of 470nm is measured, deionized water is used for replacing the solution, the solution and tyrosinase are added into the tyrosinase solution, the contrast is displayed as brown, and the inhibition rate is converted.

When the addition amount of the mulberry leaf freeze-dried powder is 10-30PPM, the activity of tyrosinase can be inhibited by 50%;

when the addition amount of the mulberry leaf freeze-dried powder is 20-40PPM, the activity of 70% tyrosinase can be inhibited;

when the addition amount of the mulberry leaf freeze-dried powder is 40-60PPM, the activity of tyrosinase can be inhibited by 80%;

when the addition amount of the mulberry leaf freeze-dried powder is 50-100PPM, the activity of 90% tyrosinase can be inhibited;

comparative example 1

Otherwise, the same example as example 1 was repeated except that the rice slurry was replaced with the same weight part of starch solution without steps S2 and S3, and the obtained mulberry leaf extract was a mulberry leaf extract.

Comparative example 2

The other example was the same as example 1 except that the rice slurry was replaced with the same volume of distilled water.

Comparative example 3

The other example is the same as example 1 except that the rice slurry was replaced with the same volume of starch solution.

Comparative example 4

The other steps are the same as example 1, except that the steps of enzymolysis and enzyme deactivation are not carried out, and no step is carried out, namely neutral protease is added according to the addition amount of 25000u/g of the composite powder, after enzymolysis is carried out for 25min at 35 ℃, and then enzyme deactivation is carried out by heating in water bath for 5min at 100 ℃. "

After the extracts obtained in example 1 and comparative examples 1-4 are freeze-dried into mulberry leaf freeze-dried powder, 50ppm solutions are prepared respectively to measure the DPPH removing capacity of the mulberry leaf freeze-dried powder.

The solution of example 1 inhibited 90% tyrosinase activity;

the solution of comparative example 1, scavenged 69% of DPPH radicals;

the solution of comparative example 2, which scavenges 80% of DPPH radicals;

the solution of comparative example 3, scavenged 83% of DPPH radicals;

the solution of comparative example 4 removed 75% of DPPH radicals.

The data for eliminating DPPH free radicals is the lowest value obtained within the normal experimental error range, the antioxidant capacity of the extract can be improved by adding neutral protease and rice milk obtained from the data, the generation of melanin is further prevented, and the antioxidant capacity of the mulberry leaf extracting solution can be improved by adding mulberry twigs and white mulberry root-bark.

Freeze-drying the extracts obtained in the embodiment 1 and the comparative examples 1 to 4 to obtain freeze-dried powder, preparing the freeze-dried powder with deionized water to obtain a solution with the concentration of 50ppm, adding the solution and tyrosinase into a tyrosinase solution together for reaction, measuring the absorbance value at the wavelength of 470nm, comparing the absorbance value with that obtained by adding the deionized water instead of the solution and the tyrosinase into the tyrosinase solution to show brown, and converting the inhibition rate.

The solution of example 1 inhibited 90% tyrosinase activity;

the solution of comparative example 1, inhibited the activity of 66% tyrosinase;

the solution of comparative example 2, which inhibited 75% tyrosinase activity;

the solution of comparative example 3 inhibited 85% tyrosinase activity

The solution of comparative example 4 inhibited 68% tyrosinase activity.

The above data for inhibiting tyrosinase activity is the lowest value obtained within the normal experimental error range, and the neutral protease treatment and rice milk addition obtained from the above data can improve the melanin production inhibition rate, and the mulberry twig and cortex mori can improve the melanin production inhibition rate of the mulberry leaf extract.

Example 2

The preparation method of the purslane extract comprises the following steps: grinding fresh and cleaned purslane into slurry, adding 80 wt% of 75 wt% ethanol solution, soaking at 35 ℃ under the pressure of 0.4MPa for 1 hour, adding 80 wt% of 75 wt% ethanol solution, extracting under reflux for 3 times, 1 hour each time, and concentrating by thin film evaporation to obtain the purslane extract.

Example 3

Cleaning fresh Tremella sporophore, stirring, pulverizing, adding 25 times of distilled water, heating at 100Mpa for 25min, and filtering to remove impurities to obtain Tremella sporophore extract.

Example 4

The mulberry leaf spot-fading mask liquid is mainly prepared from the following components in percentage by weight: 0.2% of the tremella fruiting body extract obtained in example 3, 0.05% of sodium phytate, 7% of 1, 3-propanediol, 0.1% of allantoin, 3% of 1, 2-pentanediol, 2% of isoprene glycol, 1% of glycerol, 1% of anisic acid, 1% of sodium hydroxide, 5% of the mulberry leaf extract obtained in example 1, 1% of the purslane extract obtained in example 2, and the balance of deionized water. The preparation method comprises the following steps:

s1, mixing the mulberry leaf extract and the purslane extract in corresponding weight parts to form a B phase;

s2, mixing glycerol, anisic acid and deionized water in the same weight parts as that of the glycerol into phase C in advance;

s3, heating deionized water to 75-80 ℃, sequentially adding the tremella fuciformis sporophore extract, sodium phytate, 1, 3-propylene glycol, allantoin, 1, 2-pentanediol and isoprene glycol in corresponding parts by weight, and stirring and mixing until the mixture is transparent and uniform; cooling to 50 ℃, adding the phase B and stirring uniformly; continuously cooling to 45 ℃, adding the phase C, stirring uniformly, cooling to 40 ℃, and stopping stirring.

Example 5

The mulberry leaf spot-fading mask liquid is mainly prepared from the following components in percentage by weight: 0.3% of the tremella fruiting body extract obtained in example 3, 0.05% of sodium phytate, 6% of 1, 3-propylene glycol, 0.14% of allantoin, 3% of 1, 2-pentanediol, 1% of isoprene glycol, 3% of glycerol, 2% of anisic acid, 1% of sodium hydroxide, 6% of the mulberry leaf extract obtained in example 1, 1% of the purslane extract obtained in example 2, and the balance of deionized water. The preparation method comprises the following steps:

s3 heating deionized water to 76 deg.C, sequentially adding corresponding parts by weight of Tremella fruiting body extract, sodium phytate, 1, 3-propylene glycol, allantoin, 1, 2-pentanediol and isoprene glycol, stirring and mixing to transparent and uniform; cooling to 50 ℃, adding the phase B and stirring uniformly; continuously cooling to 45 ℃, adding the phase C, stirring uniformly, cooling to 40 ℃, and stopping stirring.

Example 6

The mulberry leaf spot-fading mask liquid is mainly prepared from the following components in percentage by weight: 0.1% of the tremella fruiting body extract obtained in example 3, 0.06% of sodium phytate, 8% of 1, 3-propylene glycol, 0.04% of allantoin, 4% of 1, 2-pentanediol, 2% of isoprene glycol, 1% of glycerol, 3% of anisic acid, 2% of sodium hydroxide, 3% of the mulberry leaf extract obtained in example 1, 1.9% of the purslane extract obtained in example 2, and the balance of deionized water. The preparation method comprises the following steps:

s3 heating deionized water to 78 deg.C, sequentially adding corresponding parts by weight of Tremella fruiting body extract, sodium phytate, 1, 3-propylene glycol, allantoin, 1, 2-pentanediol and isoprene glycol, stirring and mixing to transparent and uniform; cooling to 50 ℃, adding the phase B and stirring uniformly; continuously cooling to 45 ℃, adding the phase C, stirring uniformly, cooling to 40 ℃, and stopping stirring.

Comparative example 5

The other example is the same as example 4 except that the extract of the fruit body of Tremella fuciformis is replaced with the same amount of distilled water.

Comparative example 6

The other example was the same as example 4 except that the purslane extract was replaced with the same weight of distilled water.

Freeze-drying the extract obtained in example 4 and comparative example 5 or 6 to obtain a lyophilized powder, mixing the lyophilized powder with deionized water to obtain a 50ppm solution, adding the solution and tyrosinase into a tyrosinase solution together for reaction, measuring the absorbance value at 470nm, comparing the absorbance value with that obtained by adding deionized water instead of the solution and tyrosinase into the tyrosinase solution to show brown, and converting the inhibition rate.

The solution of example 1 inhibited the activity of 87% tyrosinase;

the solution of comparative example 5, inhibited 65% tyrosinase activity;

the solution of comparative example 6, inhibited the activity of 63% tyrosinase;

the above data on the inhibition of tyrosinase activity are the lowest values obtained within the range of normal experimental error, and the addition of the purslane and the purslane extract obtained from the above data can increase the inhibition of melanin production.

Example 7

preparing S1 base membrane liquid: mixing deacetylated chitin, xylitol and distilled water according to the weight part ratio of 1: 100 to prepare substrate membrane liquid;

s2 mixing: mixing and stirring the facial mask solution and the base membrane solution of the embodiment 4 uniformly according to the volume ratio of 1: 10, and pouring the mixture into a mold to ensure that the thickness of the liquid is 3 mm;

s3 forming: and (4) drying the mould with the poured liquid by hot air at 50 ℃ until the mask is formed.

When the facial mask is used, the face is wetted after the face is cleaned, the facial mask is attached to the face, and water or essence is continuously beaten on the facial mask; or spraying water on the facial mask until the facial mask is absorbed by face.

Example 8

s2 mixing: mixing and stirring the facial mask solution and the base membrane solution of example 5 uniformly according to the volume ratio of 1: 13, and pouring the mixture into a mold to ensure that the thickness of the liquid is 1 mm;

s3 forming: and (5) drying the mould with the poured liquid by hot air at 55 ℃ until the mask is formed.

Example 9

s2 mixing: mixing and stirring the facial mask solution and the base membrane solution of example 6 uniformly according to the volume ratio of 1: 20, and pouring into a mold to ensure that the thickness of the liquid is 2 mm;

s3 forming: and (4) drying the mould with the poured liquid by hot air at 40 ℃ until the mask is formed.

The mask of examples 7-9 was subjected to dissolution rate measurement by the following method: the same mass of each of the small pieces of facial masks of examples 7 to 9 was cut and put in 150ml of water at 35 ℃, stirred until completely dissolved, and the time of dissolution was recorded.

Through measurement, the dissolution time of example 7, example 8 and example 9 can be obtained as follows: 13min, 10min and 8 min. It is understood that the mask of example 9 has the shortest dissolution time and the best solubility.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims

1. The preparation method of the soluble mulberry leaf spot-fading mask is characterized by comprising the following steps:

s1 preparation of facial mask liquid:

2. The method for preparing the soluble mulberry leaf spot-fading mask as claimed in claim 1, wherein the method comprises the following steps: in step S11, the preparation of the mulberry leaf extract comprises the following steps:

3. The method for preparing the soluble mulberry leaf spot-fading mask as claimed in claim 1, wherein,

in step S12, the preparation of the purslane extract includes the following steps: grinding fresh and clean purslane into slurry, adding 30-90% of ethanol, soaking for 1 hour at the temperature of 35 ℃ and under the pressure of 0.4MPa, adding 30-90% of ethanol, refluxing and extracting for 3 times, 1 hour each time, and concentrating by a thin film evaporation method to obtain the purslane extract.

4. The method for preparing the soluble mulberry leaf spot-fading mask as claimed in claim 1, wherein in the step S14, the preparation of the tremella fuciformis fruiting body extract comprises the following steps: cleaning and crushing fresh tremella sporophore, adding distilled water in an amount which is 20-30 times the weight of the fresh tremella sporophore, heating the mixture for 20-30 min under 100Mpa, and filtering to remove impurities to obtain the tremella sporophore extract.

5. The method for preparing the soluble mulberry leaf spot-fading mask as claimed in claim 1, wherein the method comprises the following steps: the mask liquid obtained in the step S14 comprises the following components in percentage by weight: 0.1-0.3% of tremella fruiting body extract, 0.03-0.06% of sodium phytate, 6-9% of 1, 3-propylene glycol, 0.03-0.15% of allantoin, 2-4% of 1, 2-pentanediol, 1-3% of isoprene glycol, 1-3% of glycerol, 1-3% of anisic acid, 1-3% of sodium hydroxide, 3-6% of mulberry leaf extract, 0.5-2% of purslane extract and the balance of deionized water.

6. The preparation method of the soluble mulberry leaf spot-fading mask as claimed in claim 5, wherein the mask solution obtained in the step S14 comprises the following components in percentage by weight: 0.2% of tremella fruiting body extract, 0.05% of sodium phytate, 7% of 1, 3-propylene glycol, 0.1% of allantoin, 3% of 1, 2-pentanediol, 2% of isoprene glycol, 1% of glycerol, 1% of anisic acid, 1% of sodium hydroxide, 5% of mulberry leaf extract, 1% of purslane extract and the balance of deionized water.