CN113117767B - Bubble dissolving unit for microfluid - Google Patents
Bubble dissolving unit for microfluid Download PDFInfo
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- CN113117767B CN113117767B CN202110373328.8A CN202110373328A CN113117767B CN 113117767 B CN113117767 B CN 113117767B CN 202110373328 A CN202110373328 A CN 202110373328A CN 113117767 B CN113117767 B CN 113117767B
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- micro
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a bubble dissolving unit for microfluid, belonging to the field of mass transfer of microfluid two-phase flow. The bubble dissolving unit comprises a plurality of micro-channels which are arranged in parallel along the length direction of the bubble dissolving unit, the side wall of each micro-channel is provided with a plurality of through holes, and the adjacent micro-channels are communicated with each other through the through holes; liquid flows in from a liquid inlet at one end of the microchannel and flows out from a liquid outlet at the other end of the microchannel. Wherein, the micro-channel simulates the xylem conduit of the plant, and the side wall through hole simulates the wall thread hole of the conduit of the plant, and is used for communicating a plurality of pipelines and providing the surface tension required by bubble capture and dissolution. The invention can eliminate bubbles in the micro-channel, prevent channel embolism and channel wall damage, ensure the stability of liquid flow, and has wide application prospect in the aspects of micro total analysis, cell culture, fuel cell, aerospace and the like; the invention has the advantages of high biocompatibility, simple manufacture, low cost and capability of realizing mass production.
Description
Technical Field
The invention belongs to the field of mass transfer of microfluid two-phase flow; in particular to a bionic structure unit for dissolving micro-fluid bubbles, which adopts a bionic means to carry out micro-nano structure design and manufacture.
Background
In the using process of micro-channels such as a micro-fluidic chip, a fuel cell, a space thruster and the like, bubbles are easily formed in the micro-channels, and the forming reasons include injection of experimental samples, air leakage of the channels, release of gas by heating and the like. Bubbles in the microchannel tend to affect the stability of the fluid flow, resulting in sudden changes in the microchannel pressure, increased channel flow resistance, damage to the chemistry of the channel surface, and even blockage of the channel, rendering the microdevice ineffective.
Common methods of bubble elimination include the use of de-bubbling devices, coating surfactants, increasing the pressure at the inlet, and the like. The de-bubbling device, however, increases the volume and operational complexity of the microdevice; coating surfactants can affect the chemical properties of the chip channel surface; increasing the pressure at the inlet may dissolve the bubbles, but increasing the pressure at once may damage the device and is not necessarily effective.
The xylem duct of the plant is easy to generate air bubbles in the adverse conditions of drought, freeze thawing alternation and the like, so that water transportation is blocked. And the grain holes between the plant guide pipes are communicated with each other, so that the water enters other guide pipes to finish hydraulic transmission. At the same time, the pores limit the diffusion of the bubbles, forcing the bubbles to dissolve until the conduit hydraulic transport is resumed. Based on the structure, the invention provides a bionic structure unit for dissolving micro-fluid bubbles.
Disclosure of Invention
In order to solve the above problems in the prior art, the present invention provides a bubble dissolving unit compatible with various micro devices, which can simply and efficiently remove bubbles in a micro channel under the condition that the liquid in the micro channel continuously flows, and effectively avoid the influence of the bubbles on the device functions. The bubble dissolving unit adopts a method of extracting a bubble dissolving structure affecting a biological micro-channel, and the side wall of the adjacent micro-channel is provided with a through hole, so that injected liquid can flow around the adjacent micro-channel when encountering blocked bubbles, liquid conveying is completed, and the stable flow of the liquid is kept. Meanwhile, the bubbles stay under the action of the adhesive force of the wall surface of the opening hole, and the bubbles are dissolved in the liquid by applying larger surface tension and liquid pressure to the bubbles through the smaller through holes, so that the aim of eliminating the bubbles is fulfilled.
The technical scheme of the invention is as follows:
a bubble dissolving unit for microfluid is in a plate shape in the whole structure, and a plurality of parallel micro-channels 2 are arranged on the bubble dissolving unit along the length direction. Two ends of each micro-channel 2 are respectively provided with a liquid inlet and a liquid outlet. Adjacent microchannels 2 are separated by side walls 4; each side wall 4 is provided with a plurality of through holes 3, and the adjacent micro channels 2 are communicated with each other through the through holes 3; when liquid meets blocking bubbles, the liquid can flow around to adjacent micro-channels to finish liquid conveying, the side wall provided with the through hole exerts adhesive force on the bubbles to stop the bubbles, and the bubbles are dissolved in the liquid or escape from the micro-channels under the action of liquid pressure and surface tension at the through hole, so that the elimination and dissolution of the bubbles are realized. Wherein the cross-sectional area of each micro-channel 2 is 5000nm2-1mm2(ii) a The wall thickness of the side wall 4 is 50nm-80 μm, and the flow area of the through hole 3 is 2500nm2-10000μm2(ii) a The flow area of any through hole 3 is smaller than the cross-sectional area of any microchannel 2.
Furthermore, the number of the micro-channels 2 is not less than two; the cross-sectional dimensions of the individual microchannels 2 may be the same or different.
Furthermore, the liquid inlets of the micro-channels 2 are independently arranged or communicated with each other; the liquid outlets of the micro-channels 2 are independently arranged or communicated with each other.
Further, the shape of the through hole 3 includes, but is not limited to, a rectangle, a circle, a triangle or a trapezoid, and at least two through holes 3 are provided on the sidewall 4.
Further, the material of the bubble-dissolving unit 1 includes, but is not limited to, silicon, glass, polydimethylsiloxane, polymethyl methacrylate, polystyrene, or polycarbonate.
The invention has the beneficial effects that: the invention provides a bubble dissolving unit for a micro-device, which can realize normal sample injection only by conventional sample injection equipment such as a micro-injection pump, can achieve the aim of eliminating bubbles without additional equipment, and achieves the effect of eliminating bubbles while injecting samples; the bubble dissolving unit provided by the invention can efficiently remove bubbles without influencing the performance of a micro device; the bubble dissolving unit is conveniently integrated in various micro devices, such as a micro pump, a micro valve, a chip and the like, has higher biocompatibility, is simple to manufacture and low in cost, and can realize mass production.
Drawings
FIG. 1 is a top view of a bubble-dissolving unit of the present invention.
Fig. 2 is a longitudinal cross-sectional view of a closed bubble-dissolving unit of the present invention.
FIG. 3 is an isometric view of a bubble-dissolving unit of the present invention.
Fig. 4 is a diagram of an embodiment of a closed bubble-dissolving unit of the present invention.
In the figure: 1, a bubble dissolving unit; 2 a microchannel; 3, through holes; 4 side walls; 5, covering a sheet; 6, sampling holes; 7 a waste liquid discharge hole; 8 a substrate; 9 a cell culture pond; 10 sample outlet channel.
Detailed Description
The following examples and drawings are included to further illustrate the embodiments of the present invention and are not intended to limit the invention thereto.
Example 1
A bubble dissolving unit for microfluid comprises 5 micro-channels 2 which are arranged in parallel along the length direction of the bubble dissolving unit. What is needed isThe cross-sectional area of the micro-channel 2 is 0.01mm2(ii) a Two ends of the micro-channel 2 are respectively provided with a liquid inlet and a liquid outlet; the adjacent micro-channels 2 are separated by side walls 4, and the wall thickness of the side walls 4 is 20 mu m; each side wall 4 is provided with a plurality of through holes 3, and the flow area of the through holes 3 is 400 mu m2The adjacent micro-channels 2 are communicated with each other through holes 3; under the condition that a complex defoaming device is not introduced and external pressure is not increased, bubbles can be efficiently removed, and the influence of the bubbles on the performance of the micro device is prevented.
As shown in FIG. 4, a microfluidic chip for dynamic cell culture comprises a cover plate 5 and a substrate 8 from top to bottom. The substrate 8 is provided with a bubble dissolving unit 1, a cell culture pool 9 and a sample outlet channel 10 which are connected in sequence; the cover plate 5 is provided with a sample inlet hole 6 corresponding to the liquid inlet of the bubble dissolving unit 1, and the cover plate 5 is provided with a waste liquid discharge hole 7 corresponding to the port of the sample outlet channel 10. When in use, the cover plate 5 is bonded and connected with the micro-channel 2 on the bubble dissolving unit 1, and the cell culture solution enters the bubble dissolving unit 1 from the sample inlet hole 6 by taking a micro-injection pump as power and flows along the micro-channel 2; the micro bubbles introduced by the culture solution are retained in the micro-channel 2 under the action of the bubble dissolving unit 1 and gradually dissolve or escape from the chip; the culture solution can still provide nutrition for the cells without interruption under the condition that any micro-channel 2 is blocked by the bubbles, and the flowing stability is maintained until the bubbles are completely eliminated and return to normal, and the bubble elimination and dissolution rate in the process is 0.02 mu l/s; the culture solution forms a bubble-free culture solution after passing through the bubble dissolving unit 1, the bubble-free culture solution flows out from a liquid outlet of the micro-channel 2 and enters the cell culture pool 9, the exchanged waste liquid flows out along the sample outlet channel 10 and is discharged out of the micro-fluidic chip from the waste liquid discharge hole 7, and the cell culture solution is replaced.
Example 2
A bubble dissolving unit for a micro device comprises 5 micro channels 2 which are arranged in parallel along the length direction of the bubble dissolving unit. The cross-sectional area of the micro-channel 2 is 1mm2(ii) a Two ends of the micro-channel 2 are respectively provided with a liquid inlet and a liquid outlet; the adjacent micro-channels 2 are separated by side walls 4, and the wall thickness of the side walls 4 is 60 mu m; a plurality of through holes 3 are arranged on each side wall 4, and the flow area of each through hole 3 is 10000μm2The adjacent micro-channels 2 are communicated with each other through holes 3; the bubble dissolving rate of the micro-fluidic chip manufactured by the bubble dissolving unit is 0.01 mul/s.
The above-mentioned embodiments only express the embodiments of the present invention, but not should be understood as the limitation of the scope of the invention patent, it should be noted that, for those skilled in the art, many variations and modifications can be made without departing from the concept of the present invention, and these all fall into the protection scope of the present invention.
Claims (8)
1. A bubble dissolving unit for microfluid is characterized in that the integral structure of the bubble dissolving unit is plate-shaped, and a plurality of parallel micro-channels (2) are arranged on the bubble dissolving unit along the length direction; two ends of each micro-channel (2) are respectively provided with a liquid inlet and a liquid outlet; adjacent micro-channels (2) are separated by side walls (4); each side wall (4) is provided with a through hole (3), and adjacent micro channels (2) are communicated with each other through the through holes (3);
wherein the cross-sectional area of the micro-channel (2) is 5000nm2-1mm2(ii) a The wall thickness of the side wall (4) is 50nm-80 μm; the flow area of the through hole (3) is 2500nm2-10000μm2And the flow area of any through hole (3) is smaller than the cross sectional area of any micro channel (2).
2. The bubble-dissolving unit according to claim 1, wherein said micro-channels (2) are not less than two; the cross-sectional dimensions of the microchannels (2) are the same or different.
3. The bubble-dissolving unit according to claim 1 or 2, wherein the number of through holes (3) on each side wall (4) is not less than two; the shape of the through hole (3) comprises a rectangle, a circle, a triangle or a trapezoid.
4. The bubble-dissolving unit according to claim 1 or 2, wherein the liquid inlets of each microchannel (2) are independently arranged or communicated with each other; the liquid outlets of the micro-channels (2) are independently arranged or communicated with each other.
5. The bubble-dissolving unit according to claim 3, wherein the liquid inlets of each microchannel (2) are independently arranged or communicated with each other; the liquid outlets of the micro-channels (2) are independently arranged or communicated with each other.
6. The bubble-dissolving unit of claim 1, 2 or 5, wherein the material of the bubble-dissolving unit includes but is not limited to silicon, glass, polydimethylsiloxane, polymethyl methacrylate, polystyrene or polycarbonate.
7. The bubble-dissolving unit of claim 3, wherein the material of the bubble-dissolving unit includes but is not limited to silicon, glass, polydimethylsiloxane, polymethyl methacrylate, polystyrene or polycarbonate.
8. The bubble-dissolving unit of claim 4, wherein the material of the bubble-dissolving unit includes but is not limited to silicon, glass, polydimethylsiloxane, polymethyl methacrylate, polystyrene or polycarbonate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110373328.8A CN113117767B (en) | 2021-04-07 | 2021-04-07 | Bubble dissolving unit for microfluid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110373328.8A CN113117767B (en) | 2021-04-07 | 2021-04-07 | Bubble dissolving unit for microfluid |
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| CN113117767A CN113117767A (en) | 2021-07-16 |
| CN113117767B true CN113117767B (en) | 2022-05-13 |
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| CN202110373328.8A Active CN113117767B (en) | 2021-04-07 | 2021-04-07 | Bubble dissolving unit for microfluid |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757864A (en) * | 2010-02-09 | 2010-06-30 | 华北电力大学 | Bubble swinging micro-mixing system |
| CN110012640A (en) * | 2019-02-21 | 2019-07-12 | 西安交通大学 | A kind of microchannel cold plates and electronic equipment with aperture partition |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003046256A1 (en) * | 2001-11-28 | 2003-06-05 | The Research Foundation Of State University Of New York | Electrochemically driven monolithic microfluidic systems |
| KR101141248B1 (en) * | 2009-10-22 | 2012-05-04 | 전남대학교산학협력단 | Microfluidic device for air bubble removal and fluid injection/removal |
| CN102213718A (en) * | 2011-03-24 | 2011-10-12 | 中国人民解放军第四军医大学 | Heat-shrinkable combined micro-channel chip, and preparation and application method |
| JP6480211B2 (en) * | 2014-02-28 | 2019-03-06 | アークレイ株式会社 | Plasma generating chip, plasma generating apparatus, and plasma spectroscopic analysis method |
| CA3095248A1 (en) * | 2018-04-18 | 2019-10-24 | University Of Leeds | Device for supporting development of a cell or tissue derived from the ovary |
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2021
- 2021-04-07 CN CN202110373328.8A patent/CN113117767B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757864A (en) * | 2010-02-09 | 2010-06-30 | 华北电力大学 | Bubble swinging micro-mixing system |
| CN110012640A (en) * | 2019-02-21 | 2019-07-12 | 西安交通大学 | A kind of microchannel cold plates and electronic equipment with aperture partition |
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| CN113117767A (en) | 2021-07-16 |
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