CN113117152A - 一种3d打印抑菌腔体修复支架的制备方法 - Google Patents

一种3d打印抑菌腔体修复支架的制备方法 Download PDF

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CN113117152A
CN113117152A CN201911372568.5A CN201911372568A CN113117152A CN 113117152 A CN113117152 A CN 113117152A CN 201911372568 A CN201911372568 A CN 201911372568A CN 113117152 A CN113117152 A CN 113117152A
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贺义凡
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Abstract

本发明属于人体支架技术领域，具体涉及一种3D打印抑菌腔体修复支架的制备方法，以聚乳酸‑乙醇酸共聚物、聚ε‑己内酯及改性海藻酸钠混合水凝胶为原料，并在制备的水凝胶腔体支架表面负载聚多巴胺涂层，应用腔体修复，具有良好的生物吸收性能和抗菌抑菌性能，可以缓慢释放活性药物，有效抑菌时间长，细胞相容性好，有促成骨性能。

Description

一种3D打印抑菌腔体修复支架的制备方法
技术领域
本发明属于人体支架技术领域，具体涉及一种人体可吸收生物支架技术领域，尤其涉及一种3D打印抑菌腔体修复支架的制备方法。
背景技术
组织工程支架材料能与组织活体细胞结合并能植入生物体的不同组织，并根据具体替代组织具备的功能的材料。为了使种子细胞增殖和分化，需要提供一个由生物材料所构成的细胞支架，支架材料相当于人工细胞外基质。组织工程支架材料包括：骨、软骨、血管、神经、皮肤和人工器官，如肝、脾、肾、膀胱等的组织支架材料。水凝胶支架能够为细胞的增殖与分化提供更接近于天然软骨细胞外基质的微环境，是软组织修复的一种理想材料。
传统的水凝胶支架制作技术无法实现个体化和复杂的几何形状，软组织工程涉及支架、细胞诱导和生成因子刺激以及合适的生物力学环境等多重因素，对力学强度、降解性能、支架几何形状等具有很高的要求。3D打印技术可在很大程度上实现这些特性的可控性，因此可能实现优良软组织工程支架的制备。但是3D打印的材料也需要满足一定的力学性能，才能具有良好的成型性；另外，目前通过3D打印制备所得的修复支架的功能较为单一，不具备抗菌性能，往往导致其在应用中容易受细菌感染而导致一系列炎症及并发症的发生。
发明内容
为解决上述技术问题，本发明提供了一种3D打印抑菌腔体修复支架的制备方法，由负载药物聚乳酸-乙醇酸共聚物(PLGA)、聚ε-己内酯及改性海藻酸钠水凝胶制备而成，并有聚多巴胺(PDA)涂层的3D打印复合腔体支架，生物吸收性能优异，具备抑菌抗菌能力，能有效抑制炎症等并发症。
为达到上述技术效果，本发明技术方案如下：
一种3D打印抑菌腔体修复支架的制备方法，其特征在于包括以下步骤：
（1）获取待修复腔体生物形态数据，建立三维数据，通过计算机建模，获得腔体3D打印模型；
（2）将负载药物的聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠混合水凝胶放入3D打印机，通过光固化3D打印制备水凝胶腔体支架；
（3）在步骤（2）所述的水凝胶腔体支架表面修饰聚多巴胺，得到抑菌腔体修复支架。
优选地，所述步骤（2）负载药物的聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠按照5:3:1~9:7:2的质量比混合。
优选地，所述步骤（2）的聚乳酸-乙醇酸共聚物负载的药物为抗生素。
优选地，所述步骤（2）的聚乳酸-乙醇酸共聚物负载的药物为万古霉素。
所述3D打印抑菌腔体修复支架的制备方法中，所述改性海藻酸钠的制备方法包括如下步骤：
（1）取海藻酸钠，配置为质量浓度为1~2%的海藻酸钠水溶液；
（2）在步骤（1）所述海藻酸钠水溶液中通入氧气，使海藻酸钠充分氧化，得到氧化海藻酸钠水溶液；
（3）在步骤（2）所得氧化海藻酸钠水溶液中加入甲基丙烯酸酐，搅拌加热，充分反应后，透析得到改性海藻酸钠溶液。
优选地，所述改性海藻酸钠的制备方法步骤（2）中，氧化海藻酸钠水溶液的pH值为7.0~7.5。
优选地，所述改性海藻酸钠的制备方法步骤（3）中，氧化海藻酸钠水溶液与甲基丙烯酸酐按照3:11的质量比混合。
优选地，所述改性海藻酸钠的制备方法步骤（3）中，氧化海藻酸钠水溶液与甲基丙烯酸酐的反应温度为20~30℃，，反应时间为0.5~1h。
本发明有益效果：
（1）本发明公开了的抑菌腔体修复支架，以聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠混合水凝胶为原料，并在制备的水凝胶腔体支架表面负载聚多巴胺涂层，应用腔体修复，具有良好的生物吸收性能和抗菌抑菌性能，可以缓慢释放活性药物，有效抑菌时间长，细胞相容性好，有促成骨性能；
（2）本发明所述的抑菌腔体修复支架，采用3D打印技术，可以实现个性化和机体化，打印精度准确，降低了人体支架的生产成本。
具体实施方法
下面通过具体实施方式，进一步说明本发明的技术方案。
实施例1
一种3D打印抑菌腔体修复支架的制备方法，包括以下步骤：
（1）获取待修复腔体生物形态数据，建立三维数据，通过计算机建模，获得腔体3D打印模型；
（2）将负载药物的聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠混合水凝胶放入3D打印机，通过光固化3D打印制备水凝胶腔体支架；其中，负载药物的聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠按照5:3:1的质量比混合；
（3）在步骤（2）所述的水凝胶腔体支架表面修饰聚多巴胺，得到抑菌腔体修复支架。
其中，所述改性海藻酸钠的制备方法包括如下步骤：
（1）取海藻酸钠，配置为质量浓度为1%的海藻酸钠水溶液；
（2）在步骤（1）所述海藻酸钠水溶液中通入氧气，使海藻酸钠充分氧化，得到pH值为7.0的氧化海藻酸钠水溶液；
（3）在步骤（2）所得氧化海藻酸钠水溶液中，按照3:11的质量比，加入甲基丙烯酸酐，搅拌加热，充分反应后，反应温度为20℃，反应时间为1h，透析得到改性海藻酸钠溶液。
实施例2
一种3D打印抑菌腔体修复支架的制备方法，包括以下步骤：
（1）获取待修复腔体生物形态数据，建立三维数据，通过计算机建模，获得腔体3D打印模型；
（2）将负载万古霉素的聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠按照9:7:2的质量比混合，制备为水凝胶后，放入3D打印机，通过光固化3D打印制备水凝胶腔体支架；
（3）在步骤（2）所述的水凝胶腔体支架表面修饰聚多巴胺，得到抑菌腔体修复支架。
其中，所述改性海藻酸钠的制备方法包括如下步骤：
（1）取海藻酸钠，配置为质量浓度为2%的海藻酸钠水溶液；
（2）在步骤（1）所述海藻酸钠水溶液中通入氧气，使海藻酸钠充分氧化，得到pH值为7.5的氧化海藻酸钠水溶液；
（3）在步骤（2）所得氧化海藻酸钠水溶液中，按照3:11的质量比，加入甲基丙烯酸酐，搅拌加热，反应温度为30℃，，反应时间为0.5h，充分反应后，透析得到改性海藻酸钠溶液。
实施例3
一种3D打印抑菌腔体修复支架的制备方法，包括以下步骤：
（1）获取待修复腔体生物形态数据，建立三维数据，通过计算机建模，获得腔体3D打印模型；
（2）将负载抗生素的聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠按照7:5:1.5的质量比混合，制备为水凝胶后，放入3D打印机，通过光固化3D打印制备水凝胶腔体支架；
（3）在步骤（2）所述的水凝胶腔体支架表面修饰聚多巴胺，得到抑菌腔体修复支架；
其中，所述改性海藻酸钠的制备方法包括如下步骤：
（1）取海藻酸钠，配置为质量浓度为1.5%的海藻酸钠水溶液；
（2）在步骤（1）所述海藻酸钠水溶液中通入氧气，使海藻酸钠充分氧化，得到pH值为7.2的氧化海藻酸钠水溶液；
（3）在步骤（2）所得氧化海藻酸钠水溶液中，照3:11的质量比，加入甲基丙烯酸酐，搅拌加热，反应温度为25℃，，反应时间为0.8h，充分反应后，透析得到改性海藻酸钠溶液。

Claims (8)

1.一种3D打印抑菌腔体修复支架的制备方法，其特征在于包括以下步骤：
（1）获取待修复腔体生物形态数据，建立三维数据，通过计算机建模，获得腔体3D打印模型；
（2）将负载药物的聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠混合水凝胶放入3D打印机，通过光固化3D打印制备水凝胶腔体支架；
（3）在步骤（2）所述的水凝胶腔体支架表面修饰聚多巴胺，得到抑菌腔体修复支架。
2.根据权利要求1所述3D打印抑菌腔体修复支架的制备方法，其特征在于：所述步骤（2）负载药物的聚乳酸-乙醇酸共聚物、聚ε-己内酯及改性海藻酸钠按照5:3:1~9:7:2的质量比混合。
3.根据权利要求1所述3D打印抑菌腔体修复支架的制备方法，其特征在于：所述步骤（2）的聚乳酸-乙醇酸共聚物负载的药物为抗生素。
4.根据权利要求3所述3D打印抑菌腔体修复支架的制备方法，其特征在于：所述步骤（2）的聚乳酸-乙醇酸共聚物负载的药物为万古霉素。
5.根据权利要求1或2所述3D打印抑菌腔体修复支架的制备方法，其特征在于，所述改性海藻酸钠的制备方法包括如下步骤：
（1）取海藻酸钠，配置为质量浓度为1~2%的海藻酸钠水溶液；
（2）在步骤（1）所述海藻酸钠水溶液中通入氧气，使海藻酸钠充分氧化，得到氧化海藻酸钠水溶液；
（3）在步骤（2）所得氧化海藻酸钠水溶液中加入甲基丙烯酸酐，搅拌加热，充分反应后，透析得到改性海藻酸钠溶液。
6.根据权利要求5所述3D打印抑菌腔体修复支架的制备方法，其特征在于：所述改性海藻酸钠的制备方法步骤（2）中，氧化海藻酸钠水溶液的pH值为7.0~7.5。
7.根据权利要求5所述3D打印抑菌腔体修复支架的制备方法，其特征在于：所述改性海藻酸钠的制备方法步骤（3）中，氧化海藻酸钠水溶液与甲基丙烯酸酐按照3:11的质量比混合。
8.根据权利要求5所述3D打印抑菌腔体修复支架的制备方法，其特征在于：所述改性海藻酸钠的制备方法步骤（3）中，氧化海藻酸钠水溶液与甲基丙烯酸酐的反应温度为20~30℃，，反应时间为0.5~1h。
CN201911372568.5A 2019-12-27 2019-12-27 一种3d打印抑菌腔体修复支架的制备方法 Pending CN113117152A (zh)

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Publication number Priority date Publication date Assignee Title
CN114015271A (zh) * 2021-11-25 2022-02-08 亚士创能科技(上海)股份有限公司 可批刮水包水多彩涂料及制备方法、施工方法及应用

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114015271A (zh) * 2021-11-25 2022-02-08 亚士创能科技(上海)股份有限公司 可批刮水包水多彩涂料及制备方法、施工方法及应用

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