CN113100442A - Royal jelly turtle peptide chewing tablet and preparation process thereof - Google Patents

Royal jelly turtle peptide chewing tablet and preparation process thereof Download PDF

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Publication number
CN113100442A
CN113100442A CN202110459633.9A CN202110459633A CN113100442A CN 113100442 A CN113100442 A CN 113100442A CN 202110459633 A CN202110459633 A CN 202110459633A CN 113100442 A CN113100442 A CN 113100442A
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chinese
turtle
royal jelly
parts
peptide
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CN113100442B (en
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张建人
陈敬群
张道平
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Hangzhou Xiaoshan Tianfu Biological Technology Co ltd
Hangzhou Zaodaotian Biotechnology Co ltd
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Hangzhou Xiaoshan Tianfu Biological Technology Co ltd
Hangzhou Zaodaotian Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/45—Addition of, or treatment with, microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/45—Addition of, or treatment with, microorganisms
    • A23L13/46—Addition of, or fermentation with fungi, e.g. yeasts; Enrichment with dried biomass other than starter cultures
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/48—Addition of, or treatment with, enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/20—Agglomerating; Granulating; Tabletting
    • A23P10/28—Tabletting; Making food bars by compression of a dry powdered mixture
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Molecular Biology (AREA)
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Abstract

The invention provides a royal jelly tortoise-turtle peptide chewing tablet and a preparation process thereof, belonging to the field of food and health care products. The chewable tablet comprises the following components in parts by weight: 20-50 parts of royal jelly freeze-dried powder, 20-50 parts of Chinese soft-shelled turtle peptide powder, 5-15 parts of Chinese grass turtle meat protein peptide powder, 5-12 parts of erythritol and 2-5 parts of an auxiliary agent. According to the invention, through reasonable proportioning of the components, the sleep can be obviously improved, the immunity is enhanced, and the effects of resisting fatigue and aging and promoting postoperative wound healing are achieved; the preparation process comprises the steps of carrying out full-component medicine application on the Chinese softshell turtle and the Chinese tortoise, and adopting the modes of enzymolysis, fermentation, vacuum concentration and vacuum drying to fully release the effective components in the Chinese softshell turtle and the Chinese tortoise, breaking through the fishy smell of the Chinese softshell turtle and the Chinese tortoise, keeping the nutrient components of the Chinese softshell turtle and the Chinese tortoise not lost, and providing the chewable tablet which has high nutrient component content, can be stored at normal temperature, is convenient to carry, is convenient to take, is easy to digest and absorb, is sanitary and safe.

Description

Royal jelly turtle peptide chewing tablet and preparation process thereof
Technical Field
The invention belongs to the field of food and health care products, and particularly relates to a royal jelly turtle peptide chewing tablet and a preparation process thereof.
Background
Royal jelly is a high protein, wherein the protein accounts for about 50% of the dry matter of royal jelly, 2/3 is albumin, 1/3 is globulin, the protein in royal jelly is more than 12, and in addition, many small peptides are contained. The royal jelly also contains fat, saccharide and acetylcholine-like substances, and various amino acids and biological hormones required by human body. Royal jelly contains 9 kinds of sterol compounds, and three kinds thereof have been identified so far, which are stigmasterol, cholesterol and sitosterol. In addition, it also contains minerals such as iron, copper, magnesium, zinc, potassium, and sodium. The royal jelly contains vitamin A and vitamin B, especially vitamin B complex. In addition, thiamine (B1), riboflavin (B2), pyridoxine (B6), vitamin B12, nicotinic acid, pantothenic acid, folic acid, biotin, inositol, vitamin C, vitamin D, etc. are mainly contained, wherein the content of pantothenic acid is highest. Royal jelly is a recognized health food in the world, and domestic and foreign researches prove that the royal jelly or royal jelly freeze-dried powder has the effects of improving sleep, enhancing appetite, improving immunity, resisting fatigue and resisting aging for more than 100 years. Royal jelly is sweet and sour in taste and neutral in nature and enters spleen and liver meridians; has effects in tonifying, strengthening body constitution, tonifying liver, and invigorating spleen; the product can be used for treating weakness after illness, infantile malnutrition, senile asthenia, infectious hepatitis, hypertension, rheumatic arthritis, duodenal ulcer, etc., and has effects of moistening dryness and relaxing bowels. The royal jelly can obviously enhance the resistance of human bodies to various pathogenic factors, promote the regeneration and the repair of viscera tissues, regulate internal secretion and metabolism, effectively increase appetite, improve sleep and promote growth and development, and has extremely strong health care function and medical treatment effect on human bodies. Royal jelly can strengthen body's resistance and promote growth, and is orally taken or injected to increase the diameter of red blood cell and the hemoglobin of reticulocyte, increase blood iron content and increase platelet number. The long-term administration of Lac Regis Apis can stimulate appetite, enhance constitution, and increase body weight of people with emaciation, and make people vigorous, sleep well, and have good mood.
In clinical medicine, royal jelly has the auxiliary effects of improving the resistance of old people and weak and sick people, improving malnutrition, hypoevolutism and skin metabolism, treating climacteric syndrome, liver diseases, diabetes, hypertension, nervous system diseases and cardiovascular diseases and resisting tumors.
The Chinese tortoise-turtle is a native variety of thousands of years in China and is an ancient organism on the earth. Symbiotic with dinosaurs hundreds of millions of years ago. Dinosaurs are extinct, and turtles survive to the present time tenaciously. The tortoise and turtle have strong vitality and longevity genes, have folk legends of eight thousand years of king tortoise and the longevity of the annual crane of the tortoise, and provide important basis for human health preservation and health care by utilizing the tortoise and turtle. For thousands of years, testudinate has accumulated a profound culture bottom in China as a health care food with food and medicine homology.
Chinese grass turtle, the tortoise. The civilian Li Shizhen records that the coccid is three hundred and sixty and the tortoise is the long in the compendium of materia Medica. Tortoise, also for the lingering case of coccid, and Tortoise can dredge meridians, so it can nourish yin by taking its meat to tonify heart, tonify kidney and enrich blood. The Chinese grass turtle meat is rich in protein, amino acid and unsaturated fatty acid, and contains various trace elements such as calcium, phosphorus, iron and various vitamins. Has effects in nourishing yin, tonifying yang, dispersing pathogen accumulation, regulating immunity, enhancing immunity, and relieving fatigue and aging. Has good effects on liver and kidney yin deficiency, postpartum weakness, rectocele, hysteroptosis, sexual hypofunction and the like.
The Chinese softshell turtle is also called soft-shelled turtle, has delicious meat taste and rich nutrition, and is a high-grade health tonic with homology of medicine and food. The traditional Chinese medicine considers that the turtle has the effects of promoting blood circulation, removing blood stasis, nourishing yin, and clearing and nourishing, and is usually used as a nourishing health food for promoting wound healing after severe illness and physical weakness or after surgical operation. The folk generally believes that the turtle can prevent and cure various diseases, has the functions of preventing and curing cancer when being eaten frequently, and particularly has the functions of curing low blood cells and low immunity of cancer patients after chemotherapy.
The soft-shelled turtle contains 18 kinds of amino acids, and is rich in unsaturated fatty acids with eicosapentaenoic acid (EPA for short) and docosahexaenoic acid (DHA for short) as main components. Also called omega-3 unsaturated fatty acid, trace elements of calcium and vitamin, particularly high vitamin B17 in turtle skirt, is an anticancer substance approved by the health organization (WHO) of the United nations.
However, the reports of the mixed use of royal jelly and tortoise-turtle peptide are few, and the retrieval Chinese patent application 2005100616211 discloses a preparation method of micron sugared radix codonopsitis, American ginseng, rhodiola root, tortoise plastron, turtle shell, chromium propolis granules, capsules and tablets. The middle-aged and elderly diabetic can obtain dozens of nutrient substances, and simultaneously, the health-care functions of nourishing yin, lowering blood sugar, resisting fatigue and enhancing immunity are strengthened because of the addition of the components of micron codonopsis pilosula powder, micron rhodiola rosea powder and the like which are known as oriental tonic treasure. However, only tortoise plastron and turtle shell are used in the application, the nutrients are not fully utilized, and the effective components are easily lost by only crushing the tortoise plastron and the turtle shell, and the tortoise plastron and the turtle shell have poor feeling after being taken and low absorption rate after being taken.
Therefore, the royal jelly terrapin peptide composition with high mouthfeel, high content of effective components and high digestibility needs to be provided.
Disclosure of Invention
Based on the problems in the prior art, the invention aims to provide the royal jelly turtle peptide chewable tablet which has high mouthfeel, high content of effective components and high digestibility and the preparation process thereof.
The invention freeze-dries royal jelly, reduces the loss of active substances, and the small molecular peptide free amino acid is prepared by carrying out enzymolysis, fermentation, vacuum concentration and vacuum drying on the Chinese grass turtle meat and the whole Chinese soft-shelled turtle. The prepared chewable tablet has the advantages of easy eating, digestion and absorption, convenient carrying, sanitation, safety, long storage time at normal temperature and the like, and provides a health food for improving sleep, resisting fatigue and aging, promoting the healing of surgical wounds and enhancing the physique by cancer chemotherapy and radiotherapy.
The technical purpose of the invention is realized by the following technical scheme:
the chewing tablet comprises the following components in parts by weight: 20-50 parts of royal jelly freeze-dried powder, 20-50 parts of Chinese soft-shelled turtle peptide powder, 5-15 parts of Chinese grass turtle meat protein peptide powder, 5-12 parts of erythritol and 2-6 parts of an auxiliary agent.
Preferably, the royal jelly turtle peptide chewable tablet comprises the following components in parts by weight: 25-45 parts of royal jelly freeze-dried powder, 25-45 parts of Chinese soft-shelled turtle peptide powder, 8-12 parts of Chinese grass turtle meat protein peptide powder, 8-10 parts of erythritol and 3-6 parts of an auxiliary agent.
Preferably, the royal jelly turtle peptide chewable tablet comprises the following components in parts by weight: 30-40 parts of royal jelly freeze-dried powder, 30-40 parts of Chinese soft-shelled turtle peptide powder, 10-12 parts of Chinese grass turtle meat protein peptide powder, 9-10 parts of erythritol and 4.5-6 parts of an auxiliary agent.
The auxiliary agent is silicon dioxide and magnesium stearate; preferably, the auxiliary agent is silicon dioxide and magnesium stearate in a mass ratio of 2: 1.
In some preferred embodiments, the mass ratio of the royal jelly freeze-dried powder, the Chinese soft-shelled turtle peptide powder and the Chinese grass turtle meat protein peptide powder is 1: 1: 0.1-0.5;
preferably, the mass ratio of the royal jelly freeze-dried powder to the peptide powder of the Chinese softshell turtle and the Chinese tortoise meat protein peptide powder is 1: 1: 0.2-0.4;
preferably, the mass ratio of the royal jelly freeze-dried powder to the peptide powder of the Chinese softshell turtle and the Chinese grass turtle meat protein peptide powder is 1: 1: 0.3;
the invention also provides a preparation method of the royal jelly turtle peptide chewable tablet, which comprises the following steps:
(1) filtering fresh high-quality royal jelly, grinding, freeze-drying under vacuum, pulverizing, and sieving with 80 mesh sieve;
(2) killing live Chinese soft-shelled turtle and Chinese grass turtle, removing internal organs and fat, cleaning, crushing into blocks, performing enzymolysis, fermentation, vacuum concentration, vacuum drying, pulverizing, and sieving with 120 mesh sieve;
(3) weighing royal jelly freeze-dried powder, Chinese soft-shelled turtle peptide powder and Chinese grass turtle meat protein peptide powder according to the formula dosage, mixing and stirring uniformly to obtain a mixture;
(4) uniformly spraying and humidifying the mixture obtained in the step (3) with 95% alcohol to prepare granules, and drying the alcohol to obtain the granules;
(5) and (3) tabletting the granules prepared in the step (4) to obtain the royal jelly turtle peptide tablets.
The specific operation steps of the freeze vacuum drying in the step (1) are as follows: the specific operation steps of the freezing vacuum drying in the step (1) are as follows: and (3) putting the ground royal jelly into a vacuum drying box with the vacuum degree of 0.07MPa-0.09MPa and the temperature of-30 ℃ to-35 ℃ for freeze vacuum drying for 20-26 hours to obtain the royal jelly freeze-dried powder.
The enzyme used in the enzymolysis step in the step (2) is selected from protease;
preferably, the protease is selected from the group consisting of a mixture of protease D and protease M;
more preferably, the enzyme is a mixture of protease M and protease D in a mass ratio of 1-2: 1;
the mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added enzyme in the enzymolysis process is 1000:1-3 (g/g).
Preferably, the mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added enzyme is 1000:1 (g/g).
The enzymolysis temperature is 50-55 ℃, and the enzymolysis time is 4-6 hours.
The fermentation strain used in the fermentation step in the step (2) is selected from one or more of bacillus subtilis and lactic acid bacteria;
preferably, the fermentation strain is selected from yeast and/or bacillus subtilis;
preferably, the mass ratio of the fermentation strain is 2-4:1, a mixture of yeast and bacillus subtilis;
the mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added strains in the fermentation process is 10000:3-5 (g/g).
Preferably, the mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added strains is 10000:3 (g/g).
The fermentation temperature is 20-25 ℃, and the fermentation time is 30-50 hours.
The vacuum concentration step in the step (2) is as follows: concentrating under the conditions of vacuum degree of-0.06 MPa-0.09MPa and temperature of 60-80 ℃ until the volume is reduced by more than two thirds;
the vacuum drying step in the step (2) is as follows: drying for 18-24 hours under the conditions that the vacuum degree is 0.075MPa-0.09MPa and the temperature is 60-75 ℃ until the water content is below 8g/100 g.
Compared with the prior art, the invention has the beneficial effects that:
(1) the chewable tablet provided by the invention can obviously improve sleep and enhance immunity through reasonable proportioning of the components, and has the effects of resisting fatigue and aging and promoting wound healing after operation;
(2) the invention has reasonable compatibility, natural components of raw materials and no added unnatural materials. In the preparation process, the Chinese soft-shelled turtle and the Chinese tortoise are subjected to full-component medicine application, and the modes of enzymolysis, fermentation, vacuum concentration and vacuum drying are adopted, so that the effective components in the Chinese soft-shelled turtle and the Chinese tortoise are fully released, the peculiar fishy smell of the Chinese soft-shelled turtle and the Chinese tortoise is broken through, the nutritional components of the Chinese soft-shelled turtle and the Chinese tortoise are kept from being lost, and the omega-3 unsaturated fatty acid, the linolenic acid, the free amino acid, the trace elements such as calcium, iron and the like and various vitamins are preserved;
(3) the chewing tablet provided by the invention has high content of nutrient components, can be stored at normal temperature, is convenient to carry and take, is easy to digest and absorb, and is sanitary and safe.
Detailed Description
The specific embodiments are only for explaining the present invention, and the present invention is not limited thereto, and those skilled in the art can make modifications without inventive contribution to the present embodiments as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Protease M and protease D described in the following examples were purchased from Tianye enzyme preparation (Jiangsu) Ltd; the yeast is purchased from Angel Yeast GmbH; bacillus subtilis was purchased from Weifang Yihao biotechnology Limited.
Example 1 chewing tablet of royal jelly, tortoise and turtle peptide
The paint comprises the following components in parts by weight: 20 parts of royal jelly freeze-dried powder, 20 parts of Chinese softshell turtle peptide powder, 5 parts of Chinese grass turtle meat protein peptide powder, 5 parts of erythritol, 1 part of silicon dioxide and 1 part of magnesium stearate.
The preparation method of the royal jelly turtle peptide chewable tablet comprises the following steps:
(1) filtering fresh high-quality Lac Regis Apis, grinding, freeze-vacuum drying the grinded Lac Regis Apis in a vacuum drying oven with vacuum degree of 0.07MPaMPa and temperature of-30 deg.C for 26 hr to obtain lyophilized Lac Regis Apis powder, pulverizing, and sieving with 80 mesh sieve;
(2) killing live Chinese soft-shelled turtles and Chinese shell turtles, removing internal organs and fat, cleaning, crushing into blocks, adding protease M and protease D in a mass ratio of 1:1, and performing enzymolysis at 55 ℃ for 7 hours to obtain zymolyte; adding a mixture of 4:1, fermenting the saccharomycetes and the bacillus subtilis for 50 hours at the temperature of 25 ℃ to obtain a fermentation product; concentrating the fermented product under the conditions of vacuum degree of 0.09MPa and temperature of 80 ℃ until the volume is reduced by two thirds to obtain concentrated solution; drying the concentrated solution at 60 deg.C under 0.075MPa for 18 hr until water content is below 8g/100g to obtain dried substance, pulverizing the dried substance, and sieving with 120 mesh sieve;
(3) weighing royal jelly freeze-dried powder, Chinese soft-shelled turtle peptide powder and Chinese grass turtle meat protein peptide powder according to the formula dosage, mixing and stirring uniformly to obtain a mixture;
(4) uniformly spraying and humidifying the mixture obtained in the step (3) with 95% alcohol to prepare granules, and drying the alcohol to obtain the granules;
(5) and (3) tabletting the granules prepared in the step (4) to obtain the royal jelly turtle peptide tablets.
The mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added enzyme in the enzymolysis process is 1000:3 (g/g).
The mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added strains in the fermentation process is 10000:3 (g/g).
Example 2 Royal jelly, testudinate and peptide chewing tablet
The paint comprises the following components in parts by weight: 50 parts of royal jelly freeze-dried powder, 50 parts of Chinese soft-shelled turtle peptide powder, 15 parts of Chinese grass turtle meat protein peptide powder, 12 parts of erythritol, 3 parts of silicon dioxide and 2 parts of magnesium stearate.
The preparation method of the royal jelly turtle peptide chewable tablet comprises the following steps:
(1) filtering fresh high-quality Lac Regis Apis, grinding, freeze-vacuum drying the grinded Lac Regis Apis in a vacuum drying oven with vacuum degree of 0.09MPa and temperature of-35 deg.C for 20 hr to obtain lyophilized Lac Regis Apis powder, pulverizing, and sieving with 80 mesh sieve;
(2) killing live Chinese soft-shelled turtles and Chinese shell turtles, removing internal organs and fat, cleaning, crushing into blocks, adding protease M and protease D in a mass ratio of 2:1, and performing enzymolysis at 50 ℃ for 4 hours to obtain zymolyte; adding a mixture of 2:1, fermenting the saccharomycetes and the bacillus subtilis for 30 hours at the temperature of 20 ℃ to obtain a fermentation product; concentrating the fermented product under vacuum degree of-0.06 MPa and temperature of 60 deg.C until the volume is reduced by two thirds to obtain concentrated solution; drying the concentrated solution at 75 deg.C under 0.09MPa for 24 hr until water content is below 8g/100g to obtain dried substance, pulverizing the dried substance, and sieving with 120 mesh sieve;
(3) weighing royal jelly freeze-dried powder, Chinese soft-shelled turtle peptide powder and Chinese grass turtle meat protein peptide powder according to the formula dosage, mixing and stirring uniformly to obtain a mixture;
(4) uniformly spraying and humidifying the mixture obtained in the step (3) with 95% alcohol to prepare granules, and drying the alcohol to obtain the granules;
(5) and (3) tabletting the granules prepared in the step (4) to obtain the royal jelly turtle peptide tablets.
The mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added enzyme in the enzymolysis process is 1000:2 (g/g).
The mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added strains in the fermentation process is 10000:4 (g/g).
Example 3 Royal jelly, testudinate and peptide chewing tablet
The paint comprises the following components in parts by weight: 25 parts of royal jelly freeze-dried powder, 25 parts of Chinese softshell turtle peptide powder, 7.5 parts of Chinese grass turtle meat protein peptide powder, 8 parts of erythritol, 2 parts of silicon dioxide and 1 part of magnesium stearate.
The preparation method of the royal jelly turtle peptide chewable tablet comprises the following steps:
(1) filtering fresh high-quality Lac Regis Apis, grinding, freeze-vacuum drying the grinded Lac Regis Apis in a vacuum drying oven with vacuum degree of 0.08MPa and temperature of-32 deg.C for 24 hr to obtain lyophilized Lac Regis Apis powder, pulverizing, and sieving with 80 mesh sieve;
(2) killing live Chinese soft-shelled turtles and Chinese shell turtles, removing internal organs and fat, cleaning, crushing into blocks, adding protease M and protease D in a mass ratio of 1:1, and performing enzymolysis at 50 ℃ for 5 hours to obtain zymolyte; adding 3: 1, fermenting the saccharomycetes and the bacillus subtilis for 40 hours at the temperature of 20 ℃ to obtain a fermentation product; concentrating the fermented product under the conditions of vacuum degree of 0.05MPa and temperature of 70 ℃ until the volume is reduced by two thirds to obtain concentrated solution; drying the concentrated solution at 70 deg.C under 0.08MPa for 20 hr until water content is below 8g/100g to obtain dried substance, pulverizing the dried substance, and sieving with 120 mesh sieve;
(3) weighing royal jelly freeze-dried powder, Chinese soft-shelled turtle peptide powder and Chinese grass turtle meat protein peptide powder according to the formula dosage, mixing and stirring uniformly to obtain a mixture;
(4) uniformly spraying and humidifying the mixture obtained in the step (3) with 95% alcohol to prepare granules, and drying the alcohol to obtain the granules;
(5) and (3) tabletting the granules prepared in the step (4) to obtain the royal jelly turtle peptide tablets.
The mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added enzyme in the enzymolysis process is 1000:1 (g/g).
The mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added strains in the fermentation process is 10000:5 (g/g).
Example 4 Royal jelly, testudinate and peptide chewing tablet
The paint comprises the following components in parts by weight: 40 parts of royal jelly freeze-dried powder, 40 parts of Chinese soft-shelled turtle peptide powder, 10 parts of Chinese grass turtle meat protein peptide powder, 10 parts of erythritol, 2 parts of silicon dioxide and 1 part of magnesium stearate.
The preparation method of the royal jelly turtle peptide chewable tablet comprises the following steps:
(1) filtering fresh high-quality Lac Regis Apis, grinding, freeze-vacuum drying the grinded Lac Regis Apis in a vacuum drying oven with vacuum degree of 0.08MPa and temperature of-32 deg.C for 24 hr to obtain lyophilized Lac Regis Apis powder, pulverizing, and sieving with 80 mesh sieve;
(2) killing live Chinese soft-shelled turtles and Chinese shell turtles, removing internal organs and fat, cleaning, crushing into blocks, adding protease M and protease D in a mass ratio of 1:1, and performing enzymolysis at 50 ℃ for 5 hours to obtain zymolyte; adding a mixture of 2:1, fermenting the saccharomycetes and the bacillus subtilis for 40 hours at the temperature of 20 ℃ to obtain a fermentation product; concentrating the fermented product under the conditions of vacuum degree of 0.05MPa and temperature of 70 ℃ until the volume is reduced by two thirds to obtain concentrated solution; drying the concentrated solution at 70 deg.C under 0.08MPa for 20 hr until water content is below 8g/100g to obtain dried substance, pulverizing the dried substance, and sieving with 120 mesh sieve;
(3) weighing royal jelly freeze-dried powder, Chinese soft-shelled turtle peptide powder and Chinese grass turtle meat protein peptide powder according to the formula dosage, mixing and stirring uniformly to obtain a mixture;
(4) uniformly spraying and humidifying the mixture obtained in the step (3) with 95% alcohol to prepare granules, and drying the alcohol to obtain the granules;
(5) and (3) tabletting the granules prepared in the step (4) to obtain the royal jelly turtle peptide tablets.
The mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added enzyme in the enzymolysis process is 1000:1 (g/g).
The mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added strains in the fermentation process is 10000:3 (g/g).
Comparative example 1
The difference from example 4 is that: the mass ratio of the royal jelly freeze-dried powder to the Chinese soft-shelled turtle peptide powder to the Chinese grass turtle meat protein peptide powder is 1: 1: 0.8 part of royal jelly freeze-dried powder 230/7 parts, Chinese soft-shelled turtle peptide powder 230/7 parts and Chinese grass turtle meat protein peptide powder 184/7 parts, and other operations and steps are the same as those in example 4.
Comparative example 2
The difference from example 4 is that: the mass ratio of the royal jelly freeze-dried powder to the Chinese soft-shelled turtle peptide powder to the Chinese grass turtle meat protein peptide powder is 1: 1: 0.2 part of royal jelly freeze-dried powder 460/11 parts, Chinese soft-shelled turtle peptide powder 460/11 parts and Chinese grass turtle meat protein peptide powder 92/11 parts, and other operations and steps are the same as those in example 4.
Comparative example 3
The difference from example 4 is that only protease D is used in the enzymatic hydrolysis step, and other operations and steps are the same as those in example 4.
Comparative example 4
The difference from example 4 is that the mass ratio of protease M to protease D in the enzymolysis step is 4:1, and other operations and steps are the same as those in example 4.
Comparative example 5
The difference from example 4 is that: only yeast was used in the fermentation step, and the other operations and steps were the same as in example 4.
Comparative example 6
The difference from example 4 is that: the mass ratio of yeast to bacillus subtilis in the fermentation step was 1:2, and the other operations and steps were the same as in example 4.
Effect test
1. Detection of free amino acid content in Royal jelly, Testudinis and Peste chewable buccal tablets prepared in examples 1-4 and comparative examples 3-6
The detection method comprises the following steps: GB5009.124-2016, see in particular Table 1 and Table 2.
TABLE 1
Figure BDA0003041915140000091
Figure BDA0003041915140000101
TABLE 2
Figure BDA0003041915140000102
According to the detection data of the above table 1-2, the royal jelly turtle peptide chewable tablet provided by the invention has the advantages of reasonable compatibility, natural components of raw materials and no addition of unnatural materials; in the preparation process, the Chinese medicinal herbs are fully mixed, and the modes of enzymolysis, fermentation, vacuum concentration and vacuum drying are adopted, so that the effective components in the Chinese medicinal herbs and the Chinese medicinal herbs are fully released, the peculiar smell of the Chinese medicinal herbs and the turtle is broken through, the loss of the nutritional components of the Chinese medicinal herbs and the turtle is kept, and free amino acids are stored, and the content of various amino acids in the royal jelly turtle peptide chewable tablet prepared in the examples 1-4 is obviously higher than that in the comparative examples 3-6, so that the change of the types of enzymes or strains in the preparation process and the content of the various amino acids can obviously influence the storage amount of the amino acids.
2. Content detection of vitamins in Royal jelly, Testudinis and Peste chewable tablet prepared in examples 1-4 and comparative examples 3-6
The detection method comprises the following steps: vitamin B1: GB 5009.84-2016; vitamin B12: GB 5413.14-2010; see in particular tables 3 and 4.
TABLE 3
Figure BDA0003041915140000111
TABLE 4
Figure BDA0003041915140000112
According to the detection data of the above table 3-4, the royal jelly turtle peptide chewable tablet provided by the invention has the advantages of reasonable compatibility, natural components of raw materials and no addition of unnatural materials; in the preparation process, the Chinese softshell turtle and the Chinese tortoise are subjected to full component medicine introduction, and the modes of enzymolysis, fermentation, vacuum concentration and vacuum drying are adopted, so that the effective components in the Chinese softshell turtle and the Chinese tortoise are fully released, the peculiar smell of the Chinese softshell turtle and the Chinese tortoise is broken through, the nutrient components of the Chinese softshell turtle are not lost, and the vitamins are stored, and the contents of the vitamin B1 and the vitamin B12 in the royal jelly turtle peptide chewable tablet prepared in the examples 1 to 4 are obviously higher than those in the comparative examples 3 to 6, so that the change of the types of enzymes or strains in the preparation process and the content of the vitamins can obviously influence the storage amount of the vitamins.
3. Content detection of trace elements in Royal jelly, Testudinis and Peste chewable buccal tablets prepared in examples 1-4 and comparative examples 3-6
The detection method comprises the following steps: first method of GB 5009.268-2016; see table 5 for details.
TABLE 5
Figure BDA0003041915140000121
According to the detection data of the table 5, the royal jelly turtle peptide chewable tablet provided by the invention has the advantages of reasonable compatibility, natural components of raw materials and no addition of unnatural materials; in the preparation process, the Chinese medicinal turtle and the Chinese medicinal tortoise are subjected to full-component medicine injection, and the modes of enzymolysis, fermentation, vacuum concentration and vacuum drying are adopted, so that the effective components in the Chinese medicinal turtle and the Chinese medicinal tortoise are fully released, the special fishy smell of the Chinese medicinal turtle and the Chinese medicinal tortoise is broken through, the loss of the nutritional components of the Chinese medicinal turtle and the Chinese medicinal tortoise is kept, and the trace elements such as iron, calcium and the like are stored, and the content of the trace elements such as iron, calcium and the like in the royal jelly turtle peptide chewable tablet prepared in the embodiment 1-4 is obviously higher than that in the comparative example 3-6, so that the variety of enzyme or strain in the preparation process is changed, and the content can obviously influence the storage amount of the trace.
4. Content detection of omega-3 unsaturated fatty acid in Royal jelly Guibie peptide chewable tablet prepared in examples 1-4 and comparative examples 3-6
The detection method comprises the following steps: the second method of GB 5009.168-2016; see table 6 for details.
TABLE 6
Figure BDA0003041915140000122
Figure BDA0003041915140000131
According to the detection data of the table 6, the royal jelly turtle peptide chewable tablet provided by the invention has the advantages of reasonable compatibility, natural components of raw materials and no addition of unnatural materials; in the preparation process, the Chinese medicinal herbs are fully mixed, and the modes of enzymolysis, fermentation, vacuum concentration and vacuum drying are adopted, so that the effective components in the Chinese medicinal herbs and the Chinese medicinal herbs are fully released, the peculiar smell of the Chinese medicinal herbs and the turtle is broken through, the nutrient components of the Chinese medicinal herbs and the turtle are not lost, and the omega-3 unsaturated fatty acid is stored, and the content of the omega-3 unsaturated fatty acid in the royal jelly turtle peptide chewable tablet prepared in the embodiment 1-4 is obviously higher than that in the comparative example 3-6, so that the variety of enzyme or strain in the preparation process is changed, and the content can obviously influence the storage amount of the omega-3 unsaturated fatty acid.
5. Anti-fatigue detection
Fatigue resistance test of Royal jelly Guibie peptide chewable tablet prepared in examples 1-4 and comparative examples 1-2
Detection of mouse negative gravity swimming time (the test method belongs to the field of conventional test method)
1.1 Experimental sample: the chewing tablets of royal jelly, testudinate and peptide prepared in examples 1-4 and comparative examples 1-2.
1.2 Experimental animals: kunming mouse, available at 18-24g from Nanjing Junceae bioengineering, Inc.
1.3 test methods:
1.3.1 experimental groups: kunming mice were randomly divided into 7 groups, 10 mice in each group were used as examples 1-4 and comparative examples 1-2, the test period was 7 days, the royal jelly turtle peptide chewing tablets prepared in examples 1-4 were administered to the groups of examples 1-4 as feed, the royal jelly turtle peptide chewing tablets prepared in comparative examples 1-2 were administered to the groups of comparative examples 1-2 as feed, and the mice were fed with free food and water for the control group using conventional feed (ordinary purchased mouse feed).
1.3.2 detection of mouse negative gravity swimming exhaustion time: on the 8 th day of the experiment, in the morning, a mouse with 5% weight lead skin loaded on the tail and root is placed in a swimming box for swimming, the water depth is not less than 30cm, the water temperature is 25 ℃, the time from the beginning of swimming to physical failure of the mouse is recorded, and the standard of the physical failure is that the mouse sinks on the water surface for more than 10 seconds and 60 minutes. The observer should keep the limbs of each mouse in motion throughout the experiment. If the mice float on the water surface and the limbs are immobile, they can be agitated nearby with a wooden stick.
1.3.3 statistical methods: the experimental data are as follows
Figure BDA0003041915140000141
Data analysis was shown using SPSS16.0 statistical analysis software. And comparing the differences among the groups by adopting one-factor variance analysis, wherein the difference is judged to have significance when P is less than 0.05, and the difference is judged to have extreme significance when P is less than 0.01.
1.4 results of the experiment, see table 7 below.
TABLE 7
Animal number (only) Swimming time (min)
Control group 10 12.69±7.25
Example 1 10 21.36±6.24*
Example 2 10 21.58±9.05*
Example 3 10 22.12±8.16*
Example 4 10 25.17±7.54**
Comparative example 1 10 18.85±9.21
Comparative example 2 10 19.08±8.43
Note: p < 0.05 compared to control, P < 0.01 compared to control.
According to the detection data of the above table 7, it can be seen that the negative gravity swimming time of the mice cannot be significantly increased in the comparative examples 1 to 2, the negative gravity swimming time of the mice can be significantly increased in the examples 1 to 3, and the negative gravity swimming time of the mice can be significantly increased in the example 4, compared with the control group, so that the royal jelly turtle peptide chewable tablet prepared in the examples 1 to 4 can be known to have the fatigue resistance performance increased.
It should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (10)

1. The chewing tablet of royal jelly, tortoise turtle and peptide is characterized in that: the paint comprises the following components in parts by weight: 20-50 parts of royal jelly freeze-dried powder, 20-50 parts of Chinese soft-shelled turtle peptide powder, 5-15 parts of Chinese grass turtle meat protein peptide powder, 5-12 parts of erythritol and 2-6 parts of an auxiliary agent;
the auxiliary agent is silicon dioxide and magnesium stearate.
2. The chewing tablet of royal jelly, tortoise plastron and peptide as claimed in claim 1, wherein: the paint comprises the following components in parts by weight: 25-45 parts of royal jelly freeze-dried powder, 25-45 parts of Chinese soft-shelled turtle peptide powder, 8-12 parts of Chinese grass turtle meat protein peptide powder, 8-10 parts of erythritol and 3-6 parts of an auxiliary agent;
the auxiliary agent is silicon dioxide and magnesium stearate with the mass ratio of 2: 1.
3. The chewing tablet of royal jelly, tortoise plastron and peptide as claimed in claim 1, wherein: the mass ratio of the royal jelly freeze-dried powder to the Chinese soft-shelled turtle peptide powder to the Chinese grass turtle meat protein peptide powder is 1: 1: 0.1-0.5.
4. The chewing tablet of royal jelly, tortoise plastron and peptide as claimed in claim 3, wherein: the mass ratio of the royal jelly freeze-dried powder to the Chinese soft-shelled turtle peptide powder to the Chinese grass turtle meat protein peptide powder is 1: 1: 0.3.
5. a method for preparing the chewing tablet of royal jelly testudinate peptide according to any one of claims 1 to 4, which is characterized in that: the method comprises the following steps:
(1) filtering fresh Lac Regis Apis, grinding, freeze-drying under vacuum, pulverizing, and sieving with 80 mesh sieve;
(2) killing live Chinese soft-shelled turtle and Chinese grass turtle, removing internal organs and fat, cleaning, crushing into blocks, performing enzymolysis, fermentation, vacuum concentration, vacuum drying, pulverizing, and sieving with 120 mesh sieve;
(3) weighing royal jelly freeze-dried powder, Chinese soft-shelled turtle peptide powder and Chinese grass turtle meat protein peptide powder according to the formula dosage, mixing and stirring uniformly to obtain a mixture;
(4) uniformly spraying and humidifying the mixture obtained in the step (3) with 95% alcohol to prepare granules, and drying the alcohol to obtain the granules;
(5) and (3) tabletting the granules prepared in the step (4) to obtain the royal jelly turtle peptide tablets.
6. The method of claim 5, wherein: the specific operation steps of the freezing vacuum drying in the step (1) are as follows: and (3) putting the ground royal jelly into a vacuum drying box with the vacuum degree of 0.07MPa-0.09MPa and the temperature of-30 ℃ to-35 ℃ for freeze vacuum drying for 20-26 hours to obtain the dried royal jelly freeze-dried powder.
7. The method of claim 5, wherein: the enzyme used in the enzymolysis step in the step (2) is selected from protease; preferably, the protease is a mixture of protease M and protease D in a mass ratio of 1-2: 1.
8. The method of claim 7, wherein: the mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added enzyme in the enzymolysis process is 1000: 1-3.
9. The method of claim 5, wherein: the fermentation strain used in the fermentation step in the step (2) is selected from one or more of yeast, bacillus subtilis and lactic acid bacteria; preferably, the fermentation strain is a mixture of saccharomycetes and bacillus subtilis in a mass ratio of 2-4: 1.
10. The method of claim 9, wherein: the mass ratio of the total mass of the Chinese soft-shelled turtles and the Chinese herbal turtles to the added strains in the fermentation process is 10000: 3-5.
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