CN113068545B - Plant pathogenic bacteria propagation and pathogenicity determination system - Google Patents
Plant pathogenic bacteria propagation and pathogenicity determination system Download PDFInfo
- Publication number
- CN113068545B CN113068545B CN202110358051.1A CN202110358051A CN113068545B CN 113068545 B CN113068545 B CN 113068545B CN 202110358051 A CN202110358051 A CN 202110358051A CN 113068545 B CN113068545 B CN 113068545B
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- far away
- bin
- cultivation bin
- wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
- A01G9/16—Dismountable or portable greenhouses ; Greenhouses with sliding roofs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
- A01G9/247—Watering arrangements
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
- A01G9/249—Lighting means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Greenhouses (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention provides a plant pathogenic bacteria propagation and pathogenicity measuring system which comprises a base and a cultivating bin, wherein the lower surface of the cultivating bin is fixedly connected with the base, the upper surface of the cultivating bin is rotatably connected with a top cover, one end of the cultivating bin, far away from the top cover, is fixedly connected with a mounting frame, one end of the cultivating bin, close to the mounting frame, is provided with an inoculation device, the inoculation device comprises an irrigation component and a collection component, the irrigation component comprises a power motor, a bacteria storage hopper and a spray head, and the lower surface of the power motor is fixedly connected with the surface of the cultivating bin. The invention increases the inoculation of the pathogenic bacteria of fungi or bacteria, improves the collection efficiency of the symptom change data of plant diseases by observing the interior of the cultivating bin of the plant disease for a long time with the assistance of the camera, monitors the occurrence and development dynamics of the plant diseases in real time, has reasonable design and simple operation, and is convenient for the inoculation, propagation and pathogenicity determination of the plant pathogenic bacteria.
Description
Technical Field
The invention relates to a plant pathogenic bacteria cultivation device, in particular to a plant pathogenic bacteria propagation and pathogenicity measuring system.
Background
Plant diseases refer to the phenomenon that plants are affected by biological or non-biological pathogenic factors, the normal physiological and biochemical functions of the plants are interfered, and the growth and development of the plants are affected, so that pathological changes appear on physiological or tissue structures, and various abnormal states, namely, morbid states and even death are shown. Fungi and bacteria are the main pathogenic factors causing plant diseases, and fungal diseases and bacterial diseases are important limiting factors in the agricultural production process, causing serious economic loss. The reproduction and pathogenicity determination of pathogenic bacteria are the precondition of the research on plant etiology and are also the basis of the breeding of resistant varieties of crops. Some plant pathogenic bacteria belong to obligate parasitic pathogenic bacteria, can not be cultured by artificial culture medium, and can only be propagated on host plants. At present, in order to better research the pathogenicity of plant pathogenic bacteria and evaluate the disease resistance of crop varieties, the plants need to be cultivated for a long time after the pathogenic bacteria are inoculated, the occurrence and development processes of diseases are observed, the plants are inoculated and cultivated in the existing greenhouse mode, the bacterial liquid is sprayed manually, the uniformity is not enough, the test accuracy is affected if the uniformity is not uniform, the disease resistance of a plurality of crop varieties needs to be measured in the cultivation environment, the balance of the cultivation environment cannot be ensured on the basis of the existing conditions, the pathogenicity measurement result is not accurate, the pathogenicity difference of different pathogenic bacteria cannot be effectively evaluated, the disease resistance of different crop varieties cannot be identified, the pathogenic bacteria have slight change due to the characteristic of the pathogenic bacteria, the difference cannot be effectively distinguished through the observation of human eyes, and the time-staged manual observation cannot ensure the recording of the whole disease development process.
Disclosure of Invention
In view of the above-mentioned disadvantages of the prior art, the present invention provides a system for breeding pathogenic bacteria of plants and determining pathogenicity of the pathogenic bacteria of plants, which is used for breeding pathogenic bacteria of plants, determining pathogenicity of plants and identifying disease resistance of crop varieties.
To achieve the above and other related objects, the present invention provides a system for determining the propagation and virulence of plant pathogenic bacteria, comprising: the lower surface of the cultivation bin is fixedly connected with the base, the upper surface of the cultivation bin is rotatably connected with a top cover, one end of the cultivation bin, far away from the top cover, is fixedly connected with a mounting frame, and one end of the cultivation bin, close to the mounting frame, is provided with an inoculation device;
the inoculation device comprises an irrigation assembly and a collection assembly;
the irrigation assembly comprises a power motor, a fungus storage hopper and a spray head, and fungus solution stored in the fungus storage hopper is sprayed out of the spray head onto plants; the lower surface of the power motor is fixedly connected with the surface of the cultivation bin;
the device comprises a power motor, a connecting belt, a guide wheel, a fixing block and a fixing block, wherein the connecting belt is sleeved and connected at a driving end of the power motor, one end, far away from the power motor, of the connecting belt is sleeved and connected with the guide wheel, one end, far away from the connecting belt, of the guide wheel is rotatably connected with the surface of a cultivation bin, the upper surface of the cultivation bin is slidably connected with the mounting plate, one end, close to the connecting belt, of the mounting plate is fixedly connected with the fixing block, and one end, far away from the mounting plate, of the fixing block is inserted and connected with the surface of the connecting belt;
The collection assembly comprises a light supplement lamp and a camera and is used for photographing plants in the cultivation bin;
the surface of the base is provided with an air interchanger which comprises an installation cover, and the surface of the installation cover is fixedly connected with the inner wall of the cultivation bin;
the air interchanger comprises a wind power assembly and a drawing-off assembly.
Preferably, the one end that the fixed block was kept away from to the mounting panel is rotated and is connected with the pulley, the surface of pulley and the sliding surface connection of mounting bracket, the inner wall sliding connection of mounting panel has the connecting plate, the fixed surface of connecting plate is connected with liquid conveyer, liquid conveyer's one end fixedly connected with pipe, the surface of pipe and the fixed surface of connecting plate are connected.
Preferably, the one end fixedly connected with fixed spring that liquid conveyer was kept away from to the connecting plate, the one end fixedly connected with cover block that the connecting plate was kept away from to fixed spring, the surface of cover block is established with the inner wall cover of mounting panel and is connected, the fixed surface of cover block is connected with the pull rod, the surface sliding connection of the surface of pull rod and mounting panel.
Preferably, the collection subassembly includes the stabilizer bar, the stabilizer bar is connected with the fixed surface who cultivates the storehouse, the quantity of stabilizer bar is two, and is the symmetry setting, cultivate one side fixedly connected with ratch that the storehouse is close to the stabilizer bar, the surface cover of stabilizer bar is established and is connected with the horizontal pole, the surface of horizontal pole and the lower surface sliding connection of ratch.
Preferably, the fixed surface of horizontal pole is connected with the moving motor, the drive end fixedly connected with gear of moving motor, the surface of gear is connected with the surface meshing of ratch, one side sliding connection that the horizontal pole is close to the ratch has the slide, the fixed surface of slide is connected with driving motor, driving motor's drive end fixedly connected with pivot, the surface of pivot is connected with the inner wall rotation of horizontal pole.
Preferably, the lower fixed surface of slide is connected with the cylinder, the one end fixedly connected with steadying frame of slide is kept away from to the cylinder, the one end fixedly connected with steady piece of cylinder is kept away from to the steadying frame, the lower fixed surface of steady piece is connected with the light filling lamp, the quantity of light filling lamp is four, the inner wall of steady piece rotates and is connected with the camera, the inner wall sliding connection of cylinder has the telescopic link, the one end fixedly connected with lamp shade of cylinder is kept away from to the telescopic link, the inner wall of lamp shade and the surface sliding connection of steady piece.
Preferably, the wind-force subassembly includes wind-force motor, wind-force motor's drive end cover is established and is connected with the commentaries on classics area, the rotation area is kept away from wind-force motor's one end cover and is established and be connected with the flabellum, the surface of flabellum and the surface rotation of cultivating the storehouse are connected.
Preferably, the drawing-away assembly comprises an air box, a suction motor is fixedly connected to the upper surface of the air box, a driving end of the suction motor is rotatably connected with a sleeve belt, and the surface of the sleeve belt is connected with the inner wall of the air box in a sliding mode.
Preferably, the inner wall of bellows rotates and is connected with the spiral leaf, the one end that suction motor was kept away from to the shroud is established with the surperficial cover of spiral leaf and is connected, the bellows is close to the one end fixedly connected with blast pipe of cultivating the storehouse, the surface of blast pipe and the surperficial through connection who cultivates the storehouse, the one end fixedly connected with filtration mouth of cultivating the storehouse is kept away from to the blast pipe.
As described above, the system for measuring the propagation and pathogenicity of plant pathogenic bacteria according to the present invention has at least the following advantageous effects: in conclusion, the method has simple operation, increases the stability of the sprayed bacteria liquid, realizes uniform spraying of the bacteria liquid through the connecting plate, avoids the problems that the prior method for cultivating plants in a greenhouse mode is adopted, the bacteria liquid is sprayed manually, the uniformity affects the accuracy of the test, and the pathogenicity determination of pathogenic bacteria and the disease resistance identification of crop varieties cause unsuccessful problems, improves the acquisition work of the symptom change data of plant diseases by the aid of a camera for observing the interior of a cultivating bin of the cultivating bin for a long time, monitors the occurrence and development of the plant diseases in real time, ensures that some pathogenic bacteria belong to fungi or bacteria and special parasitic pathogenic bacteria which cannot be cultivated through artificial culture media, ensures that the pathogenic bacteria propagate on host plants, mostly tests the pathogenicity of the existing pathogenic bacteria through greenhouse operation, easily generates a large amount of harmful gas in the cultivating process, need get rid of, but the unable effectual inside waste gas that gets rid of environment in the big-arch shelter, this device has realized cultivating the inside air conversion in storehouse with higher speed through setting up the flabellum, bellows and spiral leaf, has ensured to cultivate the gaseous stability of storehouse inside, guarantees to cultivate the stability of environment.
Drawings
FIG. 1 is a schematic perspective view of a system for determining the proliferation and pathogenicity of plant pathogenic bacteria according to the present invention.
FIG. 2 shows an exploded view of FIG. 1 in a system for determining the proliferation and virulence of plant pathogens in accordance with the present invention.
FIG. 3 is a schematic structural diagram of an inoculation device of the plant pathogenic bacteria propagation and pathogenicity measuring system of the invention.
FIG. 4 is a schematic view of the structure of the nozzle of the system for detecting the propagation and pathogenicity of plant pathogens according to the present invention.
FIG. 5 is a schematic diagram of the structure at A in FIG. 4 in the system for detecting the propagation and pathogenicity of plant pathogens according to the present invention.
FIG. 6 is an exploded view of the inoculating device of the system for determining the propagation and pathogenicity of plant pathogenic bacteria of the present invention.
FIG. 7 is a schematic view showing the structure of the ventilation device in the system for measuring the proliferation and pathogenicity of plant pathogens according to the present invention.
Fig. 8 is a schematic structural diagram of a wind turbine in the system for detecting the propagation and pathogenicity of plant pathogenic bacteria according to the present invention.
FIG. 9 is a schematic view showing a part of the structure of the ventilation device in the system for measuring the proliferation and pathogenicity of plant pathogens according to the present invention.
Description of the element reference numerals
1. A base; 2. a cultivation bin; 3. a top cover; 4. a mounting frame;
5. an inoculation device; 501. a power motor; 502. a connecting belt; 503. a guide wheel; 511. mounting a plate; 512. a fixed block; 513. a pulley; 521. a connecting plate; 522. a liquid conveyor; 523. a conduit; 524. a bacteria storage hopper; 525. a transverse tube; 526. a spray head; 531. fixing the spring; 532. sleeving blocks; 533. a pull rod; 541. a stabilizer bar; 542. a rack bar; 551. a cross bar; 552. a moving motor; 553. a gear; 561. a slide plate; 562. a drive motor; 563. a rotating shaft; 571. a cylinder; 572. a stabilizer frame; 573. a stabilizing block; 574. a light supplement lamp; 575. a camera; 581. a telescopic rod; 582. a lamp shade;
6. a ventilation device; 601. mounting a cover; 602. a wind motor; 603. rotating the belt; 604. a fan blade; 611. an air box; 612. a suction motor; 613. sleeving a belt; 614. helical leaves; 615. an exhaust pipe; 616. and (4) filtering the filter opening.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
Please refer to fig. 1 to 9. It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only used for matching with the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions under which the present invention can be implemented, so that the present invention has no technical significance, and any structural modification, ratio relationship change, or size adjustment should still fall within the scope of the present invention without affecting the efficacy and the achievable purpose of the present invention. In addition, the terms "upper", "lower", "middle" and "one" are used herein for the sake of clarity only, and are not intended to limit the scope of the present invention, and the relative relationship between the terms and the terms should be considered as the scope of the present invention without substantial change in the technical spirit.
The following examples are for illustrative purposes only. The various embodiments may be combined, and are not limited to what is presented in the following single embodiment.
The first embodiment is as follows:
referring to fig. 1, the present invention provides a system for determining the propagation and pathogenicity of plant pathogens, which comprises: base 1 and cultivation storehouse 2 cultivate the lower surface and the 1 fixed connection of base in storehouse 2, and the upper surface rotation of cultivating storehouse 2 is connected with top cap 3, cultivates the one end fixedly connected with mounting bracket 4 that top cap 3 was kept away from to storehouse 2, and the one end of cultivating storehouse 2 and being close to mounting bracket 4 is provided with inoculation device 5.
The inoculation device 5 comprises an irrigation component and a collection component; the collection assembly is used for photographing plants in the cultivation bin (2);
the irrigation assembly comprises a power motor 501, a bacteria storage hopper 524 and a spray head 526, wherein the fungus solution stored in the bacteria storage hopper 524 is sprayed to the plants from the spray head 526; the lower surface of the power motor 501 is fixedly connected with the surface of the cultivating bin 2.
The driving end of the power motor 501 is sleeved with a connecting belt 502, one end of the connecting belt 502 far away from the power motor 501 is sleeved with a guide wheel 503, one end of the guide wheel 503 far away from the connecting belt 502 is rotatably connected with the surface of the cultivation bin 2, the upper surface of the cultivation bin 2 is slidably connected with a mounting plate 511, one end of the mounting plate 511 close to the connecting belt 502 is fixedly connected with a fixing block 512, and one end of the fixing block 512 far away from the mounting plate 511 is inserted into the surface of the connecting belt 502 to be connected. The collection assembly comprises a fill light 574 and a camera 575.
The surface of base 1 is provided with breather 6, and breather 6 includes installation cover 601, and the surface of installation cover 601 and the inner wall fixed connection who cultivates storehouse 2.
The air interchanger 6 comprises a wind power component and a drawing component;
specifically, when in use, the pull rod 533 is pulled to fix the spring 531 to deform under stress, the sleeve block 532 is stressed to tighten, when the connecting plate 521 can be placed on the inner wall of the mounting plate 511, the pull rod 533 is aligned with the inner wall of the mounting groove on the surface of the mounting plate 511 to be placed in, the pull rod 533 is immediately loosened, the fixing spring 531 is loosened to immediately push the sleeve block 532 into the inner wall of the mounting plate 511 after losing the constraint, the connecting plate 521 is fixed, the bacteria solution is immediately added into the bacteria storage hopper 524, after the bacteria solution is filled, the power motor 501 is opened to immediately pull the connecting belt 502, the mounting plate 511 is stressed to move transversely, the liquid conveyor 522 is immediately opened to enable the bacteria solution to enter the transverse pipe through the guide pipe 523, the liquid conveyor 522 mainly has the on-off control function, and in order to realize pressurization, a water pump can be arranged, so that the liquid discharged from the spray head can be sprayed onto vegetation in the cultivating bin 2 through the spray head 526, and at present, in order to better determine the pathogenicity of plant pathogenic bacteria, the plant needs to be cultivated and observed for a long time, the existing greenhouse type plant cultivation method is not uniform enough, the test accuracy is affected due to unevenness, the disease resistance of a plurality of crop varieties needs to be measured in the cultivation environment, the balance of the cultivation environment cannot be ensured on the basis of the existing conditions, the pathogenicity measurement result is not accurate, the pathogenicity difference of different pathogenic bacteria cannot be effectively and accurately evaluated, and the disease resistance of different crop varieties can be identified, the variation is slight due to the characteristics of the pathogenic bacteria, the difference cannot be effectively distinguished through the observation of human eyes, the whole disease development process cannot be ensured to be recorded due to the fact that the manual observation has time stage, the device stably sprays the pathogenic bacteria by arranging the mounting plate 511 and the power motor 501, the cultivation stability is ensured, and the disease symptom variation data is collected in real time through the camera 575, the plant disease occurrence and development dynamics can be monitored in real time. Referring to fig. 3 and 4, one end of the mounting plate 511, which is far away from the fixed block 512, is rotatably connected with a pulley 513, the surface of the pulley 513 is slidably connected with the surface of the mounting frame 4, the inner wall of the mounting plate 511 is slidably connected with a connecting plate 521, the surface of the connecting plate 521 is fixedly connected with a liquid conveyer 522, one end of the liquid conveyer 522 is fixedly connected with a guide pipe 523, and the surface of the guide pipe 523 is fixedly connected with the surface of the connecting plate 521.
A pulley 513 is arranged, when the power motor 501 rotates, the mounting plate 511 is forced to slide, and the pulley 513 is forced to rotate with the surface of the mounting rack 4.
Referring to fig. 5, a fixed spring 531 is fixedly connected to an end of the connecting plate 521 away from the liquid conveyer 522, a sleeve block 532 is fixedly connected to an end of the fixed spring 531 away from the connecting plate 521, a surface of the sleeve block 532 is sleeved on an inner wall of the mounting plate 511, a pull rod 533 is fixedly connected to a surface of the sleeve block 532, and a surface of the pull rod 533 is slidably connected to a surface of the mounting plate 511.
The sleeve block 532 is arranged, when the bacteria storage bucket 524 of the bacteria storage bucket 524 and a pipeline need to be cleaned, the pull rod 533 is pulled to fix the top spring to be tightened under stress, the sleeve block 532 is separated from the inner wall of the mounting plate 511 immediately, the connecting plate 521 is pulled out after being bound, and the disassembly and cleaning are facilitated.
Referring to fig. 6, gather the subassembly and include stabilizer bar 541, stabilizer bar 541 is connected with the fixed surface who cultivates storehouse 2, and stabilizer bar 541's quantity is two, and is the symmetry setting, cultivates storehouse 2 and is close to one side fixedly connected with ratch 542 of stabilizer bar 541, and stabilizer bar 541's surface cover is established and is connected with horizontal pole 551, the surface of horizontal pole 551 and the lower surface sliding connection of ratch 542.
Set up stabilizer bar 541, stabilizer bar 541 passes through spacingly when horizontal pole 551 removes, the overall stability of guarantee equipment.
Referring to fig. 7, a moving motor 552 is fixedly connected to the surface of the cross bar 551, a gear 553 is fixedly connected to the driving end of the moving motor 552, the surface of the gear 553 is engaged with the surface of the rack 542, a sliding plate 561 is slidably connected to one side of the cross bar 551 close to the rack 542, a driving motor 562 is fixedly connected to the surface of the sliding plate 561, a rotating shaft 563 is fixedly connected to the driving end of the driving motor 562, and the surface of the rotating shaft 563 is rotatably connected to the inner wall of the cross bar 551.
The rotating shaft 563 is arranged, so that the sliding plate 561 can move horizontally conveniently, and the effectiveness of operation of the sliding plate is guaranteed.
Referring to fig. 6 and 7, an air cylinder 571 is fixedly connected to the lower surface of the sliding plate 561, one end of the air cylinder 571, which is far away from the sliding plate 561, is fixedly connected to a stabilizing frame 572, one end of the stabilizing frame 572, which is far away from the air cylinder 571, is fixedly connected to a stabilizing block 573, the lower surface of the stabilizing block 573 is fixedly connected to light-compensating lamps 574, the number of the light-compensating lamps 574 is four, the inner wall of the stabilizing block 573 is rotatably connected to a camera 575, the inner wall of the air cylinder 571 is slidably connected to an expansion link 581, one end of the expansion link 581, which is far away from the air cylinder 571, is fixedly connected to a lamp cover 582, and the inner wall of the lamp cover 582 is slidably connected to the outer surface of the stabilizing block 573.
Set up lamp shade 582, when only to single vegetation when needs are shot, single plant can be lived to lamp shade 582 downwards, then shoots, avoids the camera to shoot a plurality of plants alternately for the problem of the work load increase of intelligent reason picture in later stage.
Referring to fig. 8 and 9, the wind power assembly includes a wind power motor 602, a driving end of the wind power motor 602 is sleeved with a rotating belt 603, one end of the rotating belt 603 far away from the wind power motor 602 is sleeved with a fan 604, and the surface of the fan 604 is rotatably connected with the surface of the cultivation bin 2.
The fan 604 is set, the wind motor 602 is turned on to drive the rotating belt 603 to rotate, and then the fan 604 is driven to rotate, so that the outside air is sucked into the cultivating chamber 2, and the exhaust gas is pushed to the other side of the cultivating chamber 2.
Referring to fig. 8 and 9, the drawing-away assembly includes a bellows 611, a suction motor 612 is fixedly connected to an upper surface of the bellows 611, a driving end of the suction motor 612 is rotatably connected to a strap 613, and a surface of the strap 613 is slidably connected to an inner wall of the bellows 611.
A bellows 611 is provided, and when the fan 604 blows the exhaust gas to the bellows 611, the suction motor 612 is turned on to drive the spiral blade 614, and the exhaust gas is sucked into the bellows 611.
Referring to fig. 9, a spiral blade 614 is rotatably connected to the inner wall of the bellows 611, one end of the sleeve belt 613, which is far away from the suction motor 612, is sleeved on the surface of the spiral blade 614, an exhaust pipe 615 is fixedly connected to one end of the bellows 611, which is near to the cultivation bin 2, the surface of the exhaust pipe 615 is connected to the surface of the cultivation bin 2 in a penetrating manner, and a filter port 616 is fixedly connected to one end of the exhaust pipe 615, which is far away from the cultivation bin 2.
Specifically, when the device is used, the wind power motor 602 is turned on to drive the rotating belt 603, the fan blades 604 are driven, air inside the cultivation bin 2 moves towards the air box 611, external air is sucked into the cultivation bin 2, the suction motor 612 is turned on immediately, the sleeve belt 613 is forced to rotate to drive the spiral blades 614, suction force is generated immediately to suck waste gas into the air box 611, and the waste gas is discharged out of the cultivation bin 2 through the exhaust pipe 615 and the filter port 616.
To sum up, the method is simple to operate, the stability of spraying the bacteria liquid is improved, the bacteria liquid is uniformly sprayed through the connecting plate 521, the problems that the existing method for cultivating plants in a greenhouse mode is adopted, the bacteria liquid is sprayed manually, the test accuracy is affected due to insufficient uniformity, and the pathogenicity of pathogenic bacteria is unsuccessful in measuring the pathogenicity of the pathogenic bacteria are solved, the pathogenic bacteria belong to fungi or bacteria, the pathogenic bacteria cannot be found in a short time through the observation of human eyes, the change of the pathogenicity of the pathogenic bacteria can not be found through the long-time observation of the inside of the cultivating bin 2 through the assistance of the camera 575, the collection work of the symptom change data of the plant diseases is improved, the occurrence and development dynamics of the plant diseases are monitored in real time, because harmful gas generated in the cultivating process needs to be removed in time, the ventilation effect is achieved through the fan blade 604 and the wind box 611, the stability of the cultivating environment is ensured, and the pathogenicity measurement of the existing pathogenic bacteria mostly passes through the greenhouse operation, easily produce a large amount of harmful gas at cultivating the in-process, need get rid of, but the unable effectual inside waste gas of getting rid of ground environment in the big-arch shelter, this device is through setting up flabellum 604, and bellows 611 and spiral leaf 614 have realized cultivating the inside air conversion in storehouse 2 with higher speed, have ensured to cultivate the gaseous stability of 2 inside in storehouse, guarantee to cultivate the stability of environment.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Those skilled in the art can modify or change the above-described embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (4)
1. A plant pathogenic bacteria propagation and pathogenicity measuring system is characterized by comprising: the cultivation device comprises a base (1) and a cultivation bin (2), wherein the lower surface of the cultivation bin (2) is fixedly connected with the base (1), the upper surface of the cultivation bin (2) is rotatably connected with a top cover (3), one end, far away from the top cover (3), of the cultivation bin (2) is fixedly connected with a mounting frame (4), and one end, close to the mounting frame (4), of the cultivation bin (2) is provided with an inoculation device (5);
the inoculation device (5) comprises an irrigation component and a collection component;
the irrigation assembly comprises a power motor (501), a bacterium storage hopper (524) and a spray head (526), wherein the lower surface of the power motor (501) is fixedly connected with the surface of the cultivation bin (2); the fungus solution stored in the fungus storage hopper (524) is sprayed to plants from a spray head (526);
A connecting belt (502) is sleeved and connected at the driving end of the power motor (501), a guide wheel (503) is sleeved and connected at one end, far away from the power motor (501), of the connecting belt (502), one end, far away from the connecting belt (502), of the guide wheel (503) is rotatably connected with the surface of the cultivation bin (2), the upper surface of the cultivation bin (2) is slidably connected with a mounting plate (511), a fixing block (512) is fixedly connected at one end, close to the connecting belt (502), of the mounting plate (511), and one end, far away from the mounting plate (511), of the fixing block (512) is inserted and connected with the surface of the connecting belt (502); one end, far away from the fixed block (512), of the mounting plate (511) is rotatably connected with a pulley (513), the surface of the pulley (513) is in sliding connection with the surface of the mounting frame (4), the inner wall of the mounting plate (511) is in sliding connection with a connecting plate (521), the surface of the connecting plate (521) is fixedly connected with a liquid conveyor (522), one end of the liquid conveyor (522) is fixedly connected with a guide pipe (523), and the surface of the guide pipe (523) is fixedly connected with the surface of the connecting plate (521); one end, far away from the liquid conveyor (522), of the connecting plate (521) is fixedly connected with a fixed spring (531), one end, far away from the connecting plate (521), of the fixed spring (531) is fixedly connected with a sleeve block (532), the surface of the sleeve block (532) is sleeved and connected with the inner wall of the mounting plate (511), the surface of the sleeve block (532) is fixedly connected with a pull rod (533), and the surface of the pull rod (533) is in sliding connection with the surface of the mounting plate (511);
The collection assembly comprises a light supplement lamp (574) and a camera (575), and is used for photographing plants in the cultivation bin (2); the collecting assembly comprises two stabilizing rods (541), the stabilizing rods (541) are fixedly connected with the surface of the cultivating bin (2), the two stabilizing rods (541) are symmetrically arranged, one side, close to the stabilizing rods (541), of the cultivating bin (2) is fixedly connected with a toothed bar (542), a cross bar (551) is sleeved on the surface of each stabilizing rod (541), and the surface of each cross bar (551) is in sliding connection with the lower surface of the toothed bar (542); the surface of the cross rod (551) is fixedly connected with a moving motor (552), the driving end of the moving motor (552) is fixedly connected with a gear (553), the surface of the gear (553) is meshed with the surface of the toothed rod (542), one side of the cross rod (551), which is close to the toothed rod (542), is slidably connected with a sliding plate (561), the surface of the sliding plate (561) is fixedly connected with a driving motor (562), the driving end of the driving motor (562) is fixedly connected with a rotating shaft (563), and the surface of the rotating shaft (563) is rotatably connected with the inner wall of the cross rod (551); the lower surface of the sliding plate (561) is fixedly connected with an air cylinder (571), one end, far away from the sliding plate (561), of the air cylinder (571) is fixedly connected with a stabilizing frame (572), one end, far away from the air cylinder (571), of the stabilizing frame (572) is fixedly connected with a stabilizing block (573), the lower surface of the stabilizing block (573) is fixedly connected with a light supplementing lamp (574), the number of the light supplementing lamps (574) is four, the inner wall of the stabilizing block (573) is rotatably connected with a camera (575), the inner wall of the air cylinder (571) is slidably connected with a telescopic rod (581), one end, far away from the air cylinder (571), of the telescopic rod (581) is fixedly connected with a lamp shade (582), and the inner wall of the lamp shade (582) is slidably connected with the outer surface of the stabilizing block (573);
The surface of the base (1) is provided with an air interchanger (6), the air interchanger (6) comprises an installation cover (601), and the surface of the installation cover (601) is fixedly connected with the inner wall of the cultivation bin (2);
the ventilation device (6) comprises a wind power assembly and a drawing-off assembly.
2. The system for detecting the propagation and pathogenicity of plant pathogenic bacteria according to claim 1, characterized in that: the wind power assembly comprises a wind power motor (602), a rotating belt (603) is sleeved at the driving end of the wind power motor (602), a fan blade (604) is sleeved at one end, away from the wind power motor (602), of the rotating belt (603), and the surface of the fan blade (604) is rotationally connected with the surface of the cultivation bin (2).
3. The system for detecting the propagation and pathogenicity of plant pathogenic bacteria according to claim 1, characterized in that: the drawing-away assembly comprises a wind box (611), a suction motor (612) is fixedly connected to the upper surface of the wind box (611), a driving end of the suction motor (612) is rotatably connected with a sleeve belt (613), and the surface of the sleeve belt (613) is in sliding connection with the inner wall of the wind box (611).
4. A system for determining the propagation and pathogenicity of phytopathogen according to claim 3, wherein the inner wall of the air box (611) is rotatably connected with a spiral blade (614), one end of the sleeve belt (613) far away from the suction motor (612) is sleeved and connected with the surface of the spiral blade (614), one end of the air box (611) near the cultivation bin (2) is fixedly connected with an exhaust pipe (615), the surface of the exhaust pipe (615) is connected with the surface of the cultivation bin (2) in a penetrating manner, and one end of the exhaust pipe (615) far away from the cultivation bin (2) is fixedly connected with a filter (616).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110358051.1A CN113068545B (en) | 2021-04-01 | 2021-04-01 | Plant pathogenic bacteria propagation and pathogenicity determination system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110358051.1A CN113068545B (en) | 2021-04-01 | 2021-04-01 | Plant pathogenic bacteria propagation and pathogenicity determination system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113068545A CN113068545A (en) | 2021-07-06 |
| CN113068545B true CN113068545B (en) | 2022-06-28 |
Family
ID=76614626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110358051.1A Active CN113068545B (en) | 2021-04-01 | 2021-04-01 | Plant pathogenic bacteria propagation and pathogenicity determination system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113068545B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114354606B (en) * | 2022-03-21 | 2022-06-03 | 广东省农业科学院植物保护研究所 | System and method for monitoring influence of virus on plant |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWM480855U (en) * | 2014-02-20 | 2014-07-01 | Univ Far East | Plant cultivation box with separated mist supply |
| CN107118941A (en) * | 2017-03-30 | 2017-09-01 | 山东大学齐鲁医院 | A kind of medical test microbial cultivation device |
| CN107699483A (en) * | 2017-10-30 | 2018-02-16 | 北华大学 | A kind of medical test microbial cultivation equipment |
| CN208739762U (en) * | 2018-08-13 | 2019-04-16 | 长沙博立电子商务有限公司 | A kind of automatic nurturing an environment climate box of edible mushroom with sterilizing function |
| CN210746354U (en) * | 2019-09-30 | 2020-06-16 | 王本峰 | Air interchanger for agricultural greenhouse |
| CN212610611U (en) * | 2020-05-19 | 2021-02-26 | 山西省农业科学院作物科学研究所 | Plant disease-resistant identification culture observation box |
| CN112385451A (en) * | 2020-10-19 | 2021-02-23 | 北京唐冠天朗科技开发有限公司 | Intelligent agricultural management system |
-
2021
- 2021-04-01 CN CN202110358051.1A patent/CN113068545B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113068545A (en) | 2021-07-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208425277U (en) | A kind of seedling fostering equipment | |
| CN113068545B (en) | Plant pathogenic bacteria propagation and pathogenicity determination system | |
| KR101326269B1 (en) | Automatic air collection chamber for the measurement of stem respiration rate of plants | |
| CN208462542U (en) | A kind of monitoring device of Common Sage Herb cultivation | |
| CN116859007B (en) | Automatic monitoring device for growth condition of grass seeds | |
| CN115669425A (en) | Wisdom is hanging type cultivation device for agricultural | |
| CN208047497U (en) | A kind of seedling cultivation device | |
| CN101331826A (en) | Back-pack electric air-flow cotton, fruit picking machine | |
| CN105724348A (en) | Rice planthopper field capturing and collecting device and use method thereof | |
| CN208159445U (en) | Air purifier for plants based in vitro cuttings technology | |
| CN109105065A (en) | A kind of seedling culture device of the agricultural convenient for transplanting seedling plants | |
| CN209314667U (en) | A kind of aerial insect attraction solarium | |
| CN221152208U (en) | Seedling raising cabinet for agricultural vegetable cultivation | |
| CN220235567U (en) | Organic vegetable seedling raising device | |
| CN110352755A (en) | A kind of seedling growth culture apparatus convenient for transplanting and observing | |
| CN221152361U (en) | Be used for detoxify tissue culture device of shaddock class stem tip | |
| CN109197261A (en) | A kind of working method of seedling culture device of the agricultural convenient for transplanting seedling plants | |
| CN217722171U (en) | Kiwi fruit is planted and uses seedling culture device | |
| CN205357871U (en) | Collection system is caught in planthopper field | |
| CN217241714U (en) | Strawberry seedling growing device | |
| CN213404301U (en) | Afforestation lawn breeding device | |
| CN214206567U (en) | Novel real-time monitoring device for exploring plant growth cycle | |
| CN219146270U (en) | Wild environment imitation device for breeding Thuja koraiensis | |
| CN218773035U (en) | Planting seed planting characteristic observation device | |
| CN210580253U (en) | A breeding device for farming |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |