CN113068545A - Plant pathogenic bacteria propagation and pathogenicity measuring system - Google Patents

Plant pathogenic bacteria propagation and pathogenicity measuring system Download PDF

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Publication number
CN113068545A
CN113068545A CN202110358051.1A CN202110358051A CN113068545A CN 113068545 A CN113068545 A CN 113068545A CN 202110358051 A CN202110358051 A CN 202110358051A CN 113068545 A CN113068545 A CN 113068545A
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fixedly connected
bin
propagation
far away
cultivation bin
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CN113068545B (en
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于琳
佘小漫
何自福
汤亚飞
蓝国兵
李正刚
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Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
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Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/14Greenhouses
    • A01G9/16Dismountable or portable greenhouses ; Greenhouses with sliding roofs
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/24Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/24Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
    • A01G9/247Watering arrangements
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/24Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
    • A01G9/249Lighting means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Forests & Forestry (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • Cell Biology (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Greenhouses (AREA)
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Abstract

The invention provides a plant pathogenic bacteria propagation and pathogenicity measuring system which comprises a base and a cultivating bin, wherein the lower surface of the cultivating bin is fixedly connected with the base, the upper surface of the cultivating bin is rotatably connected with a top cover, one end of the cultivating bin, far away from the top cover, is fixedly connected with a mounting frame, one end of the cultivating bin, close to the mounting frame, is provided with an inoculation device, the inoculation device comprises an irrigation component and a collection component, the irrigation component comprises a power motor, a bacteria storage hopper and a spray head, and the lower surface of the power motor is fixedly connected with the surface of the cultivating bin. The invention increases the inoculation of the pathogenic bacteria of fungi or bacteria, improves the collection efficiency of the symptom change data of plant diseases by observing the interior of the cultivating bin of the plant disease for a long time with the assistance of the camera, monitors the occurrence and development dynamics of the plant diseases in real time, has reasonable design and simple operation, and is convenient for the inoculation, propagation and pathogenicity determination of the plant pathogenic bacteria.

Description

Plant pathogenic bacteria propagation and pathogenicity measuring system
Technical Field
The invention relates to a plant pathogenic bacteria cultivation device, in particular to a plant pathogenic bacteria propagation and pathogenicity measuring system.
Background
Plant diseases refer to the phenomenon that plants are affected by biological or non-biological pathogenic factors, the normal physiological and biochemical functions of the plants are interfered, and the growth and development of the plants are affected, so that pathological changes appear on physiological or tissue structures, and various abnormal states, namely, morbid states and even death are shown. Fungi and bacteria are the main pathogenic factors causing plant diseases, and fungal diseases and bacterial diseases are important limiting factors in the agricultural production process, causing serious economic loss. The breeding and pathogenicity determination of pathogenic bacteria are the precondition of plant etiology research and the basis of crop resistant variety breeding. Some plant pathogenic bacteria belong to obligate parasitic pathogenic bacteria, can not be cultured by artificial culture medium, and can only be propagated on host plants. At present, in order to better research the pathogenicity of plant pathogenic bacteria and evaluate the disease resistance of crop varieties, the plants need to be cultivated for a long time after the pathogenic bacteria are inoculated, the occurrence and development processes of diseases are observed, the plants are inoculated and cultivated in the existing greenhouse mode, the bacterial liquid is sprayed manually, the uniformity is not enough, the test accuracy is affected if the uniformity is not uniform, the disease resistance of a plurality of crop varieties needs to be measured in the cultivation environment, the balance of the cultivation environment cannot be ensured on the basis of the existing conditions, the pathogenicity measurement result is not accurate, the pathogenicity difference of different pathogenic bacteria cannot be effectively evaluated, the disease resistance of different crop varieties cannot be identified, the pathogenic bacteria have slight change due to the characteristic of the pathogenic bacteria, the difference cannot be effectively distinguished through the observation of human eyes, and the time-staged manual observation cannot ensure the recording of the whole disease development process.
Disclosure of Invention
In view of the above-mentioned disadvantages of the prior art, the present invention provides a system for breeding pathogenic bacteria of plants and determining pathogenicity of the pathogenic bacteria of plants, which is used for breeding pathogenic bacteria of plants, determining pathogenicity of plants and identifying disease resistance of crop varieties.
To achieve the above and other related objects, the present invention provides a system for determining the propagation and virulence of plant pathogenic bacteria, comprising: the lower surface of the cultivation bin is fixedly connected with the base, the upper surface of the cultivation bin is rotatably connected with a top cover, one end of the cultivation bin, far away from the top cover, is fixedly connected with a mounting frame, and one end of the cultivation bin, close to the mounting frame, is provided with an inoculation device;
the inoculation device comprises an irrigation assembly and a collection assembly;
the irrigation assembly comprises a power motor, a fungus storage hopper and a spray head, and fungus solution stored in the fungus storage hopper is sprayed out of the spray head onto plants; the lower surface of the power motor is fixedly connected with the surface of the cultivation bin;
the device comprises a power motor, a driving end of the power motor, a connecting belt, a guide wheel, a mounting plate, a fixing block and a connecting belt, wherein the driving end of the power motor is sleeved and connected with the connecting belt, one end of the connecting belt, far away from the power motor, is sleeved and connected with the guide wheel, one end of the guide wheel, far away from the connecting belt, is rotatably connected with the surface of a cultivation bin, the upper surface of the cultivation bin is connected with the mounting plate in a sliding manner, one end;
the collection assembly comprises a light supplement lamp and a camera and is used for photographing plants in the cultivation bin;
the surface of the base is provided with an air interchanger which comprises an installation cover, and the surface of the installation cover is fixedly connected with the inner wall of the cultivation bin;
the air interchanger comprises a wind power assembly and a drawing-off assembly.
Preferably, the one end that the fixed block was kept away from to the mounting panel is rotated and is connected with the pulley, the surface of pulley and the surperficial sliding connection of mounting bracket, the inner wall sliding connection of mounting panel has the connecting plate, the fixed surface of connecting plate is connected with liquid conveyer, liquid conveyer's one end fixedly connected with pipe, the surface of pipe and the fixed surface of connecting plate are connected.
Preferably, the one end fixedly connected with fixed spring that liquid conveyer was kept away from to the connecting plate, the one end fixedly connected with nested piece that the connecting plate was kept away from to fixed spring, the surface of nested piece is established with the inner wall cover of mounting panel and is connected, the fixed surface of nested piece is connected with the pull rod, the surface sliding connection of the surface of pull rod and mounting panel.
Preferably, the collection subassembly includes the stabilizer bar, the stabilizer bar is connected with the fixed surface who cultivates the storehouse, the quantity of stabilizer bar is two, and is the symmetry setting, cultivate one side fixedly connected with ratch that the storehouse is close to the stabilizer bar, the surface cover of stabilizer bar is established and is connected with the horizontal pole, the surface of horizontal pole and the lower surface sliding connection of ratch.
Preferably, the fixed surface of horizontal pole is connected with the moving motor, the drive end fixedly connected with gear of moving motor, the surface of gear is connected with the surface meshing of ratch, one side sliding connection that the horizontal pole is close to the ratch has the slide, the fixed surface of slide is connected with driving motor, driving motor's drive end fixedly connected with pivot, the surface of pivot is connected with the inner wall rotation of horizontal pole.
Preferably, the lower fixed surface of slide is connected with the cylinder, the one end fixedly connected with steadying frame of slide is kept away from to the cylinder, the one end fixedly connected with stabilizing block of cylinder is kept away from to the steadying frame, the lower fixed surface of stabilizing block is connected with the light filling lamp, the quantity of light filling lamp is four, the inner wall of stabilizing block rotates and is connected with the camera, the inner wall sliding connection of cylinder has the telescopic link, the one end fixedly connected with lamp shade of cylinder is kept away from to the telescopic link, the inner wall of lamp shade and the surface sliding connection of stabilizing block.
Preferably, the wind power component comprises a wind power motor, a rotating belt is sleeved and connected with a driving end of the wind power motor, a fan blade is sleeved and connected with one end, far away from the wind power motor, of the rotating belt, and the surface of the fan blade is rotatably connected with the surface of the cultivation bin.
Preferably, the drawing assembly comprises an air box, the upper surface of the air box is fixedly connected with a suction motor, the driving end of the suction motor is rotatably connected with a sleeve belt, and the surface of the sleeve belt is in sliding connection with the inner wall of the air box.
Preferably, the inner wall of bellows rotates and is connected with the spiral leaf, the cover area is kept away from the one end of suction motor and is established with the surperficial cover of spiral leaf and be connected, bellows is close to the one end fixedly connected with blast pipe of cultivating the storehouse, the surface of blast pipe and the surperficial through connection who cultivates the storehouse, the one end fixedly connected with filtration mouth of cultivating the storehouse is kept away from to the blast pipe.
As described above, the system for detecting the propagation and pathogenicity of plant pathogenic bacteria according to the present invention has at least the following advantages: in conclusion, the method is simple to operate, the stability of the sprayed bacteria liquid is improved, the uniform spraying of the bacteria liquid is realized through the connecting plate, the problems that the conventional method for cultivating plants in a greenhouse mode is adopted, the bacteria liquid is sprayed manually, the test accuracy is affected due to insufficient uniformity and unevenness, and the pathogenicity determination of pathogenic bacteria and the disease resistance identification of crop varieties are unsuccessful are solved, the collection work of plant disease symptom change data is improved through the long-time observation of the interior of a cultivating bin of the cultivating bin by the aid of a camera, the occurrence and development of plant diseases are monitored in real time, the pathogenic bacteria belong to fungi or bacteria, some pathogenic bacteria belong to special parasitic pathogenic bacteria and cannot be cultivated through an artificial culture medium, the propagation of the pathogenic bacteria on host plants is ensured, the pathogenicity determination of the existing pathogenic bacteria mostly passes the greenhouse operation, and a large amount of harmful gas is easily generated in the cultivating process, need get rid of, but the unable effectual inside waste gas of getting rid of environment in the big-arch shelter, this device has realized cultivating the inside air conversion in storehouse with higher speed through setting up flabellum, bellows and spiral leaf, has ensured to cultivate the gaseous stability of storehouse inside, guarantees to cultivate the stability of environment.
Drawings
FIG. 1 is a schematic perspective view of a system for determining the proliferation and pathogenicity of plant pathogens according to the present invention.
FIG. 2 shows an exploded view of FIG. 1 in a system for determining the proliferation and virulence of plant pathogens in accordance with the present invention.
FIG. 3 is a schematic structural diagram of an inoculation device of the plant pathogenic bacteria propagation and pathogenicity measuring system of the invention.
FIG. 4 is a schematic view of the structure of the nozzle of the system for detecting the propagation and pathogenicity of plant pathogens according to the present invention.
FIG. 5 is a schematic diagram of the structure at A in FIG. 4 in the system for detecting the propagation and pathogenicity of plant pathogens according to the present invention.
FIG. 6 is an exploded view of the inoculating device of the system for determining the propagation and pathogenicity of plant pathogenic bacteria of the present invention.
FIG. 7 is a schematic view showing the structure of the ventilation device in the system for measuring the proliferation and pathogenicity of plant pathogens according to the present invention.
FIG. 8 is a schematic diagram of the structure of the wind-driven generator in the system for detecting the propagation and pathogenicity of plant pathogens according to the present invention.
FIG. 9 is a schematic view showing a part of the structure of the ventilation device in the system for measuring the proliferation and pathogenicity of plant pathogens according to the present invention.
Description of the element reference numerals
1. A base; 2. a cultivation bin; 3. a top cover; 4. a mounting frame;
5. an inoculating device; 501. a power motor; 502. a connecting belt; 503. a guide wheel; 511. mounting a plate; 512. a fixed block; 513. a pulley; 521. a connecting plate; 522. a liquid conveyor; 523. a conduit; 524. a bacteria storage hopper; 525. a transverse tube; 526. a spray head; 531. fixing the spring; 532. sleeving blocks; 533. a pull rod; 541. a stabilizer bar; 542. a rack bar; 551. a cross bar; 552. a moving motor; 553. a gear; 561. a slide plate; 562. a drive motor; 563. a rotating shaft; 571. a cylinder; 572. a stabilizer frame; 573. a stabilizing block; 574. a light supplement lamp; 575. a camera; 581. a telescopic rod; 582. a lamp shade;
6. a ventilation device; 601. mounting a cover; 602. a wind motor; 603. rotating the belt; 604. a fan blade; 611. an air box; 612. a suction motor; 613. sleeving a belt; 614. helical leaves; 615. an exhaust pipe; 616. and (4) filtering the filter opening.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
Please refer to fig. 1 to 9. It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only used for matching with the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions under which the present invention can be implemented, so that the present invention has no technical significance, and any structural modification, ratio relationship change, or size adjustment should still fall within the scope of the present invention without affecting the efficacy and the achievable purpose of the present invention. In addition, the terms "upper", "lower", "middle" and "one" used in the present specification are for clarity of description only, and are not used to limit the scope of the present invention, and the relative relationship between the terms and the terms is not to be construed as the scope of the present invention.
The following examples are for illustrative purposes only. The various embodiments may be combined, and are not limited to what is presented in the following single embodiment.
The first embodiment is as follows:
referring to fig. 1, the present invention provides a system for determining the propagation and pathogenicity of plant pathogens, which comprises: base 1 and cultivation storehouse 2 cultivate the lower surface and the 1 fixed connection of base in storehouse 2, and the upper surface rotation of cultivating storehouse 2 is connected with top cap 3, cultivates the one end fixedly connected with mounting bracket 4 that top cap 3 was kept away from to storehouse 2, and the one end of cultivating storehouse 2 and being close to mounting bracket 4 is provided with inoculation device 5.
The inoculation device 5 comprises an irrigation component and a collection component; the collection assembly is used for photographing plants in the cultivation bin (2);
the irrigation assembly comprises a power motor 501, a bacteria storage hopper 524 and a spray head 526, wherein the fungus solution stored in the bacteria storage hopper 524 is sprayed to the plants from the spray head 526; the lower surface of the power motor 501 is fixedly connected with the surface of the cultivating bin 2.
The driving end of the power motor 501 is sleeved with a connecting belt 502, one end of the connecting belt 502 far away from the power motor 501 is sleeved with a guide wheel 503, one end of the guide wheel 503 far away from the connecting belt 502 is rotatably connected with the surface of the cultivation bin 2, the upper surface of the cultivation bin 2 is slidably connected with a mounting plate 511, one end of the mounting plate 511 close to the connecting belt 502 is fixedly connected with a fixing block 512, and one end of the fixing block 512 far away from the mounting plate 511 is inserted into the surface of the connecting belt 502 to be connected. The collection assembly comprises a fill light 574 and a camera 575.
The surface of base 1 is provided with breather 6, and breather 6 includes installation cover 601, and the surface of installation cover 601 and the inner wall fixed connection of breeding the storehouse 2.
The air interchanger 6 comprises a wind power component and a drawing component;
specifically, when in use, the pull rod 533 is pulled to fix the spring 531 to deform under stress, the sleeve block 532 is stressed to tighten, when the connecting plate 521 can be placed on the inner wall of the mounting plate 511, the pull rod 533 is aligned with the inner wall of the mounting groove on the surface of the mounting plate 511 and is placed in the mounting groove, the pull rod 533 is immediately loosened, the fixing spring 531 loses constraint, the sleeve block 532 is immediately pushed into the inner wall of the mounting plate 511 to complete the fixing of the connecting plate 521, the bacterial liquid is immediately added into the bacterial storage hopper 524, after the bacterial liquid is filled, the power motor 501 is turned on to immediately pull the connecting belt 502, the mounting plate 511 is stressed to move transversely, the liquid conveyor 522 is immediately opened to enable the bacterial liquid to enter the transverse pipe through the guide pipe 523, the liquid conveyor 522 mainly has the on-off control function, in order to realize pressurization, a water pump can be arranged, so that the liquid discharged from the spray head can be sprayed to vegetation in a, the plant needs to be cultivated and observed for a long time, the existing greenhouse type plant cultivation method is not uniform enough, the test accuracy is affected due to unevenness, the disease resistance of a plurality of crop varieties needs to be measured in the cultivation environment, the balance of the cultivation environment cannot be ensured on the basis of the existing conditions, the pathogenicity measurement result is not accurate, the pathogenicity difference of different pathogenic bacteria cannot be effectively and accurately evaluated, and the disease resistance of different crop varieties can be identified, the variation is slight due to the characteristics of the pathogenic bacteria, the difference cannot be effectively distinguished through the observation of human eyes, the whole disease development process cannot be ensured to be recorded due to the fact that the manual observation has time stage, the device stably sprays the pathogenic bacteria by arranging the mounting plate 511 and the power motor 501, the cultivation stability is ensured, and the disease symptom variation data is collected in real time through the camera 575, the plant disease occurrence and development dynamics can be monitored in real time. Referring to fig. 3 and 4, one end of the mounting plate 511, which is far away from the fixed block 512, is rotatably connected with a pulley 513, the surface of the pulley 513 is slidably connected with the surface of the mounting frame 4, the inner wall of the mounting plate 511 is slidably connected with a connecting plate 521, the surface of the connecting plate 521 is fixedly connected with a liquid conveyer 522, one end of the liquid conveyer 522 is fixedly connected with a guide pipe 523, and the surface of the guide pipe 523 is fixedly connected with the surface of the connecting plate 521.
A pulley 513 is provided, and when the power motor 501 rotates, the mounting plate 511 is forced to slide, and the pulley 513 is forced to rotate with the surface of the mounting frame 4.
Referring to fig. 5, a fixed spring 531 is fixedly connected to an end of the connecting plate 521, which is away from the liquid conveyor 522, a sleeve block 532 is fixedly connected to an end of the fixed spring 531, which is away from the connecting plate 521, a surface of the sleeve block 532 is sleeved on an inner wall of the mounting plate 511, a pull rod 533 is fixedly connected to a surface of the sleeve block 532, and a surface of the pull rod 533 is slidably connected to a surface of the mounting plate 511.
The sleeve block 532 is arranged, when the bacteria storage hopper 524 and the pipeline need to be cleaned, the pull rod 533 is pulled to fix the top spring to be stressed and tightened, the sleeve block 532 is separated from the inner wall of the mounting plate 511 immediately, the connecting plate 521 is pulled out after being out of constraint, and the disassembly and cleaning are convenient.
Referring to fig. 6, the collecting assembly includes stabilizer bars 541, the stabilizer bars 541 are fixedly connected to the surface of the cultivation bin 2, the number of the stabilizer bars 541 is two, and the stabilizer bars 541 are symmetrically arranged, one side of the cultivation bin 2 close to the stabilizer bars 541 is fixedly connected to a toothed bar 542, a cross bar 551 is sleeved on the surface of the stabilizer bars 541, and the surface of the cross bar 551 is slidably connected to the lower surface of the toothed bar 542.
Set up stabilizer bar 541, stabilizer bar 541 passes through spacingly when horizontal pole 551 removes, the overall stability of guarantee equipment.
Referring to fig. 7, a moving motor 552 is fixedly connected to the surface of the cross bar 551, a gear 553 is fixedly connected to the driving end of the moving motor 552, the surface of the gear 553 is engaged with the surface of the rack 542, a sliding plate 561 is slidably connected to the side of the cross bar 551 close to the rack 542, a driving motor 562 is fixedly connected to the surface of the sliding plate 561, a rotating shaft 563 is fixedly connected to the driving end of the driving motor 562, and the surface of the rotating shaft 563 is rotatably connected to the inner wall of the cross bar 551.
The rotating shaft 563 is arranged, so that the sliding plate 561 can move horizontally conveniently, and the effectiveness of operation is guaranteed.
Referring to fig. 6 and 7, an air cylinder 571 is fixedly connected to the lower surface of the sliding plate 561, a stabilizing frame 572 is fixedly connected to one end, away from the sliding plate 561, of the air cylinder 571, a stabilizing block 573 is fixedly connected to one end, away from the air cylinder 571, of the stabilizing frame 572, light supplementing lamps 574 are fixedly connected to the lower surface of the stabilizing block 573, the number of the light supplementing lamps 574 is four, a camera 575 is rotatably connected to the inner wall of the stabilizing block 573, an expansion link 581 is slidably connected to the inner wall of the air cylinder 571, a lamp cover 582 is fixedly connected to one end, away from the air cylinder 571, of the expansion link 581, and.
Set up lamp shade 582, when only to single vegetation when needs are shot, lamp shade 582 can cover single plant downwards, then shoots, avoids the camera to shoot a plurality of plants alternately for the problem of the work load increase of later stage intelligence reason picture.
Referring to fig. 8 and 9, the wind power assembly includes a wind power motor 602, a driving end of the wind power motor 602 is sleeved with a rotating belt 603, one end of the rotating belt 603 far away from the wind power motor 602 is sleeved with a fan 604, and the surface of the fan 604 is rotatably connected with the surface of the cultivation bin 2.
The fan blades 604 are arranged, the wind motor 602 is turned on to drive the rotating belt 603 to rotate, the fan blades 604 are driven to rotate, external air is sucked into the cultivating bin 2, and waste gas is pushed to the other side of the cultivating bin 2.
Referring to fig. 8 and 9, the drawing-off assembly includes a bellows 611, a suction motor 612 is fixedly connected to an upper surface of the bellows 611, a driving end of the suction motor 612 is rotatably connected to a strap 613, and a surface of the strap 613 is slidably connected to an inner wall of the bellows 611.
A bellows 611 is provided, and when the fan blades 604 blow the exhaust gas toward the bellows 611, the suction motor 612 is turned on to drive the spiral blade 614, thereby sucking the exhaust gas into the bellows 611.
Referring to fig. 9, a spiral blade 614 is rotatably connected to the inner wall of the bellows 611, one end of the sleeve belt 613, which is far away from the suction motor 612, is sleeved on the surface of the spiral blade 614, an exhaust pipe 615 is fixedly connected to one end of the bellows 611, which is near to the cultivation bin 2, the surface of the exhaust pipe 615 is connected to the surface of the cultivation bin 2 in a penetrating manner, and a filter port 616 is fixedly connected to one end of the exhaust pipe 615, which is far away from the cultivation bin 2.
Specifically, when the device is used, the wind motor 602 is turned on to drive the rotating belt 603, the fan blades 604 are driven to enable air inside the cultivation bin 2 to move towards the wind box 611, external air is sucked into the cultivation bin 2, the suction motor 612 is turned on immediately to enable the sleeve belt 613 to be forced to rotate to drive the spiral blades 614, suction force is generated immediately to suck waste gas into the wind box 611, and the waste gas is discharged out of the cultivation bin 2 through the exhaust pipe 615 and the filtering port 616.
In summary, the operation of the invention is simple, the stability of spraying the bacteria liquid is increased, the bacteria liquid is uniformly sprayed through the connecting plate 521, the problems that the prior plant cultivation in a greenhouse mode is adopted, the bacteria liquid is sprayed manually, the test accuracy is affected if the bacteria liquid is not uniform, and the pathogenicity of the pathogenic bacteria is determined unsuccessfully are solved, the pathogenic bacteria belong to fungi or bacteria, the pathogenic bacteria can not be found in a short time through the observation of human eyes, the collection work of the symptom change data of the plant diseases is improved through the long-time observation of the interior of the cultivation bin 2 by the assistance of the camera 575, the occurrence and development dynamics of the plant diseases are monitored in real time, the harmful gas generated in the cultivation process needs to be removed in time, the ventilation effect is achieved through the fan blades 604 and the wind box 611, the stability of the cultivation environment is ensured, and the pathogenicity determination of the prior pathogenic bacteria mostly, easily produce a large amount of harmful gas at cultivating the in-process, need get rid of, but inside waste gas can't effectually be got rid of to the ground environment in the big-arch shelter, and this device has realized cultivating the inside air conversion in storehouse 2 with higher speed through setting up flabellum 604, bellows 611 and spiral leaf 614, has ensured to cultivate the gaseous stability of 2 inside in storehouse, guarantees to cultivate the stability of environment.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (10)

1. A plant pathogenic bacteria propagation and pathogenicity measuring system is characterized by comprising: the cultivation device comprises a base (1) and a cultivation bin (2), wherein the lower surface of the cultivation bin (2) is fixedly connected with the base (1), the upper surface of the cultivation bin (2) is rotatably connected with a top cover (3), one end, far away from the top cover (3), of the cultivation bin (2) is fixedly connected with a mounting frame (4), and one end, close to the mounting frame (4), of the cultivation bin (2) is provided with an inoculation device (5);
the inoculation device (5) comprises an irrigation component and a collection component;
the irrigation assembly comprises a power motor (501), a bacterium storage hopper (524) and a spray head (526), wherein the lower surface of the power motor (501) is fixedly connected with the surface of the cultivation bin (2); the fungus solution stored in the fungus storage hopper (524) is sprayed to plants from a spray head (526);
the device is characterized in that a connecting belt (502) is sleeved and connected at the driving end of the power motor (501), a guide wheel (503) is sleeved and connected at one end, far away from the power motor (501), of the connecting belt (502), one end, far away from the connecting belt (502), of the guide wheel (503) is rotatably connected with the surface of the cultivation bin (2), the upper surface of the cultivation bin (2) is slidably connected with a mounting plate (511), one end, close to the connecting belt (502), of the mounting plate (511) is fixedly connected with a fixing block (512), and one end, far away from the mounting plate (511), of the fixing block (512) is inserted and connected with the surface of;
the collection assembly comprises a light supplement lamp (574) and a camera (575), and is used for photographing plants in the cultivation bin (2);
the surface of the base (1) is provided with an air interchanger (6), the air interchanger (6) comprises an installation cover (601), and the surface of the installation cover (601) is fixedly connected with the inner wall of the cultivation bin (2);
the ventilation device (6) comprises a wind power assembly and a drawing-off assembly.
2. The system for determining the propagation and virulence of plant pathogens according to claim 1, wherein the system comprises: the one end rotation that fixed block (512) was kept away from in mounting panel (511) is connected with pulley (513), the surface of pulley (513) and the surperficial sliding connection of mounting bracket (4), the inner wall sliding connection of mounting panel (511) has connecting plate (521), the fixed surface of connecting plate (521) is connected with liquid conveyer (522), the one end fixedly connected with pipe (523) of liquid conveyer (522), the fixed surface of pipe (523) is connected with the fixed surface of connecting plate (521).
3. The system for determining the propagation and virulence of plant pathogens according to claim 1, wherein the system comprises: the one end rotation that fixed block (512) was kept away from in mounting panel (511) is connected with pulley (513), the surface of pulley (513) and the surperficial sliding connection of mounting bracket (4), the inner wall sliding connection of mounting panel (511) has connecting plate (521), the fixed surface of connecting plate (521) is connected with liquid conveyer (522), the one end fixedly connected with pipe (523) of liquid conveyer (522), the fixed surface of pipe (523) is connected with the fixed surface of connecting plate (521).
4. The system according to claim 3, wherein said system is adapted to determine the level of pathogenic bacteria in said plant, said system comprising: the one end fixedly connected with fixed spring (531) of liquid conveyor (522) is kept away from in connecting plate (521), the one end fixedly connected with nested piece (532) of connecting plate (521) is kept away from in fixed spring (531), the surface of nested piece (532) is established with the inner wall cover of mounting panel (511) and is connected, the fixed surface of nested piece (532) is connected with pull rod (533), the surface sliding connection of pull rod (533) and mounting panel (511).
5. The system for determining the propagation and virulence of plant pathogens according to claim 1, wherein the system comprises: the collecting assembly comprises two stabilizing rods (541), the stabilizing rods (541) are fixedly connected with the surface of the cultivating bin (2), the two stabilizing rods (541) are symmetrically arranged, one side of the cultivating bin (2) close to the stabilizing rods (541) is fixedly connected with a toothed bar (542), a cross rod (551) is sleeved on the surface of each stabilizing rod (541), and the surface of each cross rod (551) is slidably connected with the lower surface of the toothed bar (542).
6. The system for determining the propagation and virulence of plant pathogens according to claim 5, wherein the system comprises: the surface fixed connection of horizontal pole (551) has mobile motor (552), the drive end fixedly connected with gear (553) of mobile motor (552), the surface of gear (553) is connected with the surface meshing of ratch (542), one side sliding connection that horizontal pole (551) are close to ratch (542) has slide (561), the surface fixed connection of slide (561) has driving motor (562), the drive end fixedly connected with pivot (563) of driving motor (562), the surface of pivot (563) is connected with the inner wall rotation of horizontal pole (551).
7. The system for determining the propagation and virulence of plant pathogens according to claim 6, wherein the system comprises: the lower surface of the sliding plate (561) is fixedly connected with an air cylinder (571), one end, far away from the sliding plate (561), of the air cylinder (571) is fixedly connected with a stabilizing frame (572), one end, far away from the air cylinder (571), of the stabilizing frame (572) is fixedly connected with a stabilizing block (573), the lower surface of the stabilizing block (573) is fixedly connected with a light supplementing lamp (574), the number of the light supplementing lamp (574) is four, the inner wall of the stabilizing block (573) is rotatably connected with a camera (575), the inner wall of the air cylinder (571) is slidably connected with an expansion link (581), one end, far away from the air cylinder (571), of the expansion link (581) is fixedly connected with a lamp shade (582), and the inner wall of the lamp shade (582) is slidably connected with the outer.
8. The system for determining the propagation and virulence of plant pathogens according to claim 1, wherein the system comprises: the wind power component comprises a wind power motor (602), a rotating belt (603) is sleeved and connected with a driving end of the wind power motor (602), a fan blade (604) is sleeved and connected with one end, far away from the wind power motor (602), of the rotating belt (603), and the surface of the fan blade (604) is rotatably connected with the surface of the cultivation bin (2).
9. The system for determining the propagation and virulence of plant pathogens according to claim 1, wherein the system comprises: the drawing-off assembly comprises a wind box (611), a suction motor (612) is fixedly connected to the upper surface of the wind box (611), a driving end of the suction motor (612) is rotatably connected with a sleeve belt (613), and the surface of the sleeve belt (613) is in sliding connection with the inner wall of the wind box (611).
10. A system for determining the propagation and pathogenicity of phytopathogen according to claim 9, wherein the inner wall of the air box (611) is rotatably connected with a spiral blade (614), one end of the sleeve belt (613) far away from the suction motor (612) is sleeved and connected with the surface of the spiral blade (614), one end of the air box (611) near the cultivation bin (2) is fixedly connected with an exhaust pipe (615), the surface of the exhaust pipe (615) is connected with the surface of the cultivation bin (2) in a penetrating manner, and one end of the exhaust pipe (615) far away from the cultivation bin (2) is fixedly connected with a filter (616).
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