CN113063779A

CN113063779A - Sampler and mixing device of sample and reagent

Info

Publication number: CN113063779A
Application number: CN202110278623.5A
Authority: CN
Inventors: 不公告发明人
Original assignee: Aituo Biotechnology Hangzhou Co ltd
Current assignee: Aituo Biotechnology Hangzhou Co ltd
Priority date: 2021-03-15
Filing date: 2021-03-15
Publication date: 2021-07-02

Abstract

The present disclosure relates to gene detection technology, and more particularly to a sampler and a mixing apparatus for a sample and a reagent. The first aspect discloses a sampler, including the body that has the inner chamber, the body still has the communicating opening with the inner chamber, and the lateral wall of inner chamber is provided with the sample portion, and the process that the sample collector stretched into the inner chamber contacts with the sample portion, and the sample portion takes out the sample in the sample collector. The second aspect discloses a mixing arrangement of sample and reagent, including sampler and blender, the sampler is including the body that has the inner chamber, the body still have with the communicating opening of inner chamber, the lateral wall of inner chamber is provided with the sample portion, the process that the sample collector stretched into the inner chamber contacts with the sample portion, the sample in the sample collector is taken out to the sample portion, the blender is including the body that has the cavity, the cavity communicates with each other with the inner chamber. Need not other operations when taking out the sample in by the sample collector, only need put into the body with the sample collector and can accomplish the sample, improved sampling efficiency.

Description

Sampler and mixing device of sample and reagent

Technical Field

The present disclosure relates to gene detection technology, and more particularly to a sampler and a mixing apparatus for a sample and a reagent.

Background

In the field of gene detection technology, a cotton swab is usually used for collecting a sample, and the cotton swab with the collected sample is mixed with a reagent and then analyzed by an optical analysis instrument to obtain corresponding parameters. For example, obtaining whether a sample contains a certain virus or not, etc., is widely applied in the field of epidemic disease detection.

Generally, the mixing of the sample and the reagent includes two ways, one is to mix the sample and the reagent by an external mechanical structure, for example, mixing by stirring, etc. Another is to use molecular forces between the sample and the reagent for mixing. The mixing by adopting the molecular acting force between the sample and the reagent is suitable for the mixing of trace samples, and the mixing mode is widely applied in the field of gene detection.

In the practical operation process, the sample in the cotton swab is generally taken out through the test tube, for example, a certain reagent can be stored in the test tube, the cotton swab is inserted into the test tube and stirred to disperse the sample in the cotton swab into the reagent in the test tube, and then the reagent containing the sample is mixed with other reagents to perform corresponding detection and analysis.

In the prior art, the structure of the test tube for taking out the sample in the cotton swab is unreasonable, so that the sample in the cotton swab is difficult to take out and the operation is complex. During a large-scale epidemic outbreak, the sampling mode reduces the sampling efficiency, and further reduces the detection efficiency.

Disclosure of Invention

This disclosure of first aspect provides a sampler, has solved the technical problem of the difficulty of taking a sample in the cotton swab among the prior art.

Some embodiments adopted to solve the above technical problems include:

this disclosure of first aspect provides a sampler, including the body that has the inner chamber, the body still has the communicating opening with the inner chamber, the lateral wall of inner chamber is provided with sample portion, the process that the sample collector stretched into the inner chamber with sample portion contacts, sample portion takes out the sample in the sample collector.

In practical application, the sampling part is arranged to enable the sampling part to be in contact with the sample collector while the sample collector is inserted into the tube body, so that a sample in the sample collector is taken out. Need not other operations when taking out the sample in by the sample collector, only need put into the body with the sample collector and can accomplish the sample, improved sampling efficiency, reduced operating personnel's intensity of labour.

Preferably, the tube body is provided with a seal body that closes the opening.

In this scheme, the setting of seal can prevent effectively that the sample from being contaminated, has optimized the application performance of sampler.

Preferably, the sampling part is a scraping blade arranged on the side wall of the inner cavity.

In this scheme, the manufacturing cost of sampler has been reduced for the doctor-bar to sample portion.

Preferably, the number of the scraping blades is at least two, and the at least two scraping blades are arranged along the axial direction of the pipe body.

In this scheme, the doctor-bar sets up the twice at least, is favorable to taking out the sample in the sample collector, has further improved the sample of sampler and has taken out efficiency.

Preferably, the blade is a sheet having elastic deformation capability.

In this scheme, the doctor-bar has elastic deformation ability for the doctor-bar can adapt to different sample collectors, has guaranteed the sample quality of sampler effectively, has optimized the sample performance of sampler.

Second aspect the present disclosure provides a mixing device for a sample and a reagent, which solves the technical problem of the prior art that the sampling of the sample of the mixing device is difficult.

Some embodiments adopted to solve the above technical problems include:

the utility model provides a mixing arrangement of sample and reagent, includes sampler and blender, the sampler is including the body that has the inner chamber, the body still has the opening that communicates with each other with the inner chamber, the lateral wall of inner chamber is provided with sample portion, and the sample collector stretches into the process of inner chamber with sample portion contact, sample portion takes out sample in the sample collector, the blender is including the body that has the cavity, the cavity with the inner chamber communicates with each other.

In practical use, the sampling part is arranged to enable the sampling part to be contacted with the sample collector while the sample collector is inserted into the tube body, so that a sample in the sample collector is taken out. Need not other operations when taking out the sample in by the sample collector, only need put into the body with the sample collector and can accomplish the sample, improved sampling efficiency, reduced operating personnel's intensity of labour. The sample in the inner cavity can be subjected to subsequent operation after entering the cavity, so that the sampling time is saved, and the mixing efficiency of the mixing device is improved.

Preferably, the cavity further comprises a flow channel, the inner cavity is communicated with the cavity through the flow channel, the cavity comprises a premixing cavity, a mixing cavity and a storage cavity, the flow channel is communicated with the premixing cavity through a mouth part, and at least one part of all the mouth parts are arranged in an overlapping mode.

In this scheme, the body has and mixes chamber, mixing chamber in advance, and the medium in the runner gets into the storage chamber after premixing, mixing, is located the medium misce bene of storage intracavity.

In addition, at least one part of the opening part is overlapped, so that the contact area of the media in the premixing cavity is increased, and the mixing capability of the media in the premixing cavity is optimized.

Preferably, the mixing device further comprises a sample pushing device, the sample pushing device is arranged along the length direction of the body, and the medium in the flow channel sequentially enters the premixing cavity, the mixing cavity and the storage cavity under the action of the sample pushing device.

In this scheme, push away the function that the setting of appearance ware mainly played the medium that will mix in the chamber in advance and push the storage chamber through the hybrid chamber, push away the appearance ware and can realize automatic operation, do not need artificial intervention, improved mixing arrangement's operating efficiency.

Preferably, the sample injector includes a first portion and a second portion, the body is located between the first portion and the second portion, the sample injector further includes a driver for driving the first portion and the second portion to displace along the body, and when the driver drives the first portion and the second portion to displace along the first direction, the medium located in the flow channel sequentially enters the premix chamber, the mixing chamber, and the storage chamber.

In this scheme, the setting of first portion and second part makes the push away the appearance ware and has reasonable structure, can promote this internal medium flow effectively, has optimized the application experience who pushes away the appearance ware.

Preferably, the driver is a linear motor, or the driver is an electromagnet.

In the scheme, the driver is simple in structure and high in action precision, and the performance of the driver is optimized.

Drawings

For purposes of explanation, several embodiments of the disclosed technology are set forth in the following figures. The following drawings are incorporated herein and constitute a part of the detailed description. In some instances, well-known structures and components are shown in block diagram form in order to avoid obscuring the concepts of the disclosed subject technology.

FIG. 1 is a schematic representation of the sampler of the first aspect.

Fig. 2 is a schematic view of the mixing device of the second aspect in a first orientation.

FIG. 3 is a schematic view of a second direction mixing device in a second direction.

Fig. 4 is a front view of the body in the second aspect, in which the two-dot chain line is used only to show different portions of the cavity.

Fig. 5 is a schematic view of the body of the second aspect in a first orientation.

Fig. 6 is a schematic view of a second orientation of the body in the second aspect.

Fig. 7 is a schematic view of a structure of a mixing body including a guide portion.

Fig. 8 is a cross-sectional view taken along line a-a in fig. 4.

Shown in the figure:

1. body, 2, premix chamber, 3, storage chamber, 4, mixing chamber, 41, mixture, 411, guide part, 5, runner, 511, oral part, 601, inner chamber, 602, body, 603, sample portion, 604, seal, 100, sampler, 200, mixer, 300, injector, 400, first part.

Detailed Description

The specific embodiments illustrated below are intended as descriptions of various configurations of the presently disclosed subject technology and are not intended to represent the only configurations in which the presently disclosed subject technology may be practiced. Specific embodiments include specific details for the purpose of providing a thorough understanding of the presently disclosed subject matter technology. It will be apparent, however, to one skilled in the art that the disclosed subject matter technology is not limited to the specific details shown herein and may be practiced without these specific details.

In a first aspect, referring to fig. 1, a sampler includes a tube 602 having a cavity 601, the tube 602 further has an opening communicating with the cavity 601, a sampling portion 603 is disposed on a side wall of the cavity 601, a sample collector contacts the sampling portion 603 during a process of extending into the cavity 601, and the sampling portion 603 takes out a sample in the sample collector.

The sample collector commonly used in genetic testing techniques is typically a cotton swab. The cotton swab has the characteristic of convenient sampling and is widely applied to the field of gene detection. It will be appreciated that the sample collector of the present disclosure may be a cotton swab, or other similar functional sampling structure.

The technical solution of the first aspect of the present disclosure is further described below with a cotton swab as the sample collector. When the cotton swab is used for collecting a sample, the cotton swab can rotate in the oral cavity or the nasal cavity of a human body, the body fluid of the human body is absorbed by the cotton swab, and at the moment, the cotton swab obtains the sample.

After the sample is collected by the swab, the sample is attached to the swab. The sample attached to the cotton swab cannot be effectively detected, and therefore, the sample attached to the cotton swab needs to be removed, mixed with a reagent, and reacted to detect the sample. At this point, it is necessary to extract the sample attached to the swab.

Specifically, in the practical application process of the present invention, a certain amount of reagent may be pre-stored in the inner cavity 601, and then the cotton swab is inserted into the inner cavity 601, and when the cotton swab contacts with the reagent, a part of the sample attached to the cotton swab is extracted. Because the cotton swab is simply contacted with the reagent and a proper amount of samples cannot be effectively taken, the sampling part 603 is arranged to enable the sampling part 603 to extrude the cotton swab, when the cotton swab is inserted into the inner cavity 601, the cotton swab is extruded by the sampling part 603, the sample attached to the cotton swab can be extruded by the extrusion, and the sample extraction quality is improved.

The sampler 100 that this disclosure provided only needs insert the extraction that the sample can be accomplished to inner chamber 601 with the cotton swab in the actual operation process, has the sample and draws the convenience, characteristics that operating efficiency is high.

It will be appreciated that the swab is provided as a specific embodiment and that the swab may be replaced by other sample collectors having similar characteristics during use.

Referring to fig. 1, in some embodiments, the tubular body 602 is provided with a seal 604 that closes the opening.

A portion of the tube 602 may be made of a material having elastic deformation capability. Specifically, after the cotton bud is placed in the cavity 601 to complete the sample extraction, the reagent containing the sample remains in the cavity 601. At this time, the opening is sealed by the sealing body 604, and the reagent containing the sample can be discharged to the target position through the sample discharge port of the tube 602 by pressing the tube 602, so as to facilitate the subsequent operation of the sample.

Specifically, a sample discharge port may be provided on a side wall of the tube 602, and the sample discharge port may be closed by a valve body to prevent contamination of the sample. Of course, the sample can also be discharged through the opening, and the discharging mode of the sample is not limited and can be reasonably selected according to the requirement.

In some embodiments, the sampling portion 603 is a wiper disposed on a sidewall of the cavity 601.

The number of the scraping blades is at least two, and the at least two scraping blades are arranged along the axial direction of the pipe body 602.

The scraping blade is a sheet body with elastic deformation capacity.

The sampling portion 603 may be integrated with the tube 602. The thickness of the end of the sampling portion 603 close to the tube 602 may be greater than the thickness of the end of the sampling portion 603 away from the tube 602. Generally, the greater the number of blades, the better the sampling effect of the sampling portion 603, that is, the greater the number of sampling portions 603, the greater the number of times the swab is repeatedly pressed, and the better the improved quality of the sample inside the swab.

The sampling portion 603 may have a special-shaped structure. The sampling portion 603 has a main function of varying the diameter of the cavity 601 so as to be repeatedly pressed by the sampling portion 603 during the insertion of the swab, thereby effectively extracting the sample attached to the swab.

Referring to fig. 2 to 3, in a second aspect, a sample and reagent mixing device includes a sampler 100 and a mixer 200, where the sampler 100 includes a tube 602 having a lumen 601, the tube 602 further has an opening communicating with the lumen 601, a side wall of the lumen 601 is provided with a sampling portion 603, a process of extending a sample collector into the lumen 601 contacts the sampling portion 603, the sampling portion 603 takes out a sample in the sample collector, and the mixer 200 includes a body 1 having a cavity communicating with the lumen 601.

Due to the adoption of the sampler 100 disclosed in the first aspect, the mixing device is very convenient to sample, so that the mixing efficiency of samples is improved, and the application experience of the mixing device is optimized.

Referring to fig. 4 to 8, in some embodiments, the chamber further includes a flow passage 5, the inner cavity 601 communicates with the chamber through the flow passage 5, the chamber includes a premixing chamber 2, a mixing chamber 4 and a storage chamber 3, the flow passage 5 communicates with the premixing chamber 2 through a mouth portion 511, and as shown in fig. 8, at least a part of all the mouth portions 511 are overlapped.

Wherein sampler 100 applied in the second aspect adopts the structure disclosed in the first aspect.

The side wall of the pipe 602 has a sample outlet communicated with the flow channel 5, and a membrane valve may be disposed at the sample outlet, so that the medium in the inner cavity 601 may enter the flow channel 5, and the medium in the flow channel 5 may not enter the inner cavity 601. Specifically, the membrane valve may be provided in the pipe 602, and the membrane valve may be provided in the body 1. Typically, a membrane valve is disposed in tube 602 to facilitate processing of sampler 100 and body 1, thereby reducing the manufacturing cost of the mixing device.

In some of the flow channels 5, corresponding reagents may be pre-stored, which are used to complete the reaction with the sample, so that the sample can be analyzed and detected by the optical instrument.

The operating principle of sampler 100 is described with reference to the first aspect. The working principle of the mixer 200 is as follows: after the media in the flow channels 5 have entered the premixing chamber 2, the media discharged from the different flow channels 5 are mixed together by the molecular force. In general, the larger the contact area of the media in the different flow channels 5 in the premixing chamber 2, the better the mixing performance, and therefore, the mouths of the different flow channels 5 can be arranged to overlap completely.

Can also set up the mixture in mixing chamber 4, after the medium that accomplishes the back of mixing in premixing chamber 2 gets into mixing chamber 4, under the effect of mixture 41, the medium in mixing chamber 4 can produce the turbulent effect, and under the effect of turbulent effect, there is the medium can be by homogeneous mixing in mixing chamber 4.

The medium in the mixing chamber 4 is mixed uniformly and then enters the storage chamber 3. The media located in the storage chamber 3, after having been mixed, can be optically detected and analyzed. Generally, the body 1 may be made of transparent material for the volume of the analyzer, so that the optical instrument can directly detect and analyze the medium in the storage chamber 3.

Referring to fig. 7, the shape of the mixing body 41 may be a hemisphere, or a cylinder, or a profile structure. Of course, when the mixing body 41 has a cylindrical shape, the guide portion 411 may be provided on the mixing body 41, and the guide portion 411 may be recessed or protruded into the mixing body 41. The guiding portion 411 is mainly used for guiding the medium in the mixing chamber 4, so as to further optimize the turbulent effect of the medium in the mixing chamber 4 and optimize the mixing performance of the medium.

Typically, the mixing body 41 extends into the cavity. Of course, the mixing body 41 may also be recessed into the side walls of the cavity. In addition, the body 1 can be made of membranes, and a plurality of membranes can be assembled together in a bonding mode to form the characteristics of a cavity, a flow channel and the like.

A part or all of the diaphragm may be made of a transparent material so that the storage chamber 3 has a transparent characteristic, which is advantageous for the detection and analysis of the medium in the storage chamber 3 by an optical instrument.

In some embodiments, the mixing device further includes a sample injector 300, wherein the reagent is stored in some of the flow channels 52, the sample injector 300 is disposed along the length direction of the body 1, and the media in the flow channels 5 and the storage chamber 3 sequentially enter the premix chamber 2, the mixing chamber 4 and the storage chamber 3 under the action of the sample injector 300.

The sample injector 300 comprises a first part 400 and a second part, the body 1 is located between the first part 400 and the second part, the sample injector 300 further comprises a driver for driving the first part 400 and the second part to displace along the body 1, and when the driver drives the first part 400 and the second part to displace along the first direction, the medium located in the flow channel 5 sequentially enters the premix chamber 2, the mixing chamber 4 and the storage chamber 3.

The driver is a linear motor, or the driver is an electromagnet.

When the actuator is an electromagnet, the first portion 400 and the second portion may be armatures. The second part and the driver are not shown in the figure.

The first and

second portions

400, 400 urge the media to flow during displacement in the first direction. When the first part 400 and the second part are displaced in the second direction, the medium is already stored in the storage chamber 3, so that the displacement of the first part 400 and the second part in the second direction is a null movement and has no practical effect on the medium. The first direction is opposite to the second direction.

Generally, the body 1 may be disposable, and thus, the first and

second portions

400 and 400 may not contact the body 1 during the displacement in the second direction.

The displacement of the first part 400 and the second part along the first part 400 causes a certain squeezing of the body 1, whereby the medium from the premix chamber 2 enters the reservoir chamber 3 via the mixing chamber 4.

The main function of the actuator is to displace the first part 400 from the second part, and therefore the actuator may be replaced by other structures that output linear motion.

It will be appreciated that when the body 1 is provided with a frame feature, the driver and sampler 100 may be fixed to the frame, either by gluing or other means of attachment.

While the subject matter of the present disclosure and its corresponding details have been described above, it is to be understood that the above description is only illustrative of some embodiments of the subject matter of the present disclosure and that some of the details may be omitted from the detailed description.

In addition, in some of the embodiments disclosed above, there is a possibility that a plurality of embodiments may be combined and implemented, and various combinations are not listed at length. The implementation embodiments can be freely combined according to the requirements when the technical personnel in the field carry out the implementation so as to obtain better application experience.

Other configurations of details or figures may be derived by those skilled in the art in practicing the presently disclosed subject matter, as well as figures, and it will be apparent that such details are within the scope of the presently disclosed subject matter and are covered by the presently disclosed subject matter without departing from the presently disclosed subject matter.

Claims

1. A sampler, characterized by: the sampling device comprises a pipe body (602) with an inner cavity (601), wherein the pipe body (602) is further provided with an opening communicated with the inner cavity (601), a sampling part (603) is arranged on the side wall of the inner cavity (601), the process that a sample collector extends into the inner cavity (601) is contacted with the sampling part (603), and a sample in the sample collector is taken out by the sampling part (603).

2. The sampler (100) of claim 1, wherein: the tube (602) is provided with a seal (604) closing the opening.

3. The sampler (100) of claim 2, wherein: the sampling part (603) is a scraping blade arranged on the side wall of the inner cavity (601).

4. The sampler (100) of claim 3, wherein: the number of the scraping pieces is at least two, and the at least two scraping pieces are arranged along the axial direction of the pipe body (602).

5. The sampler (100) of claim 3 or 4 wherein: the scraping blade is a sheet body with elastic deformation capacity.

6. A sample and reagent mixing device, comprising: including sampler (100) and blender (200), sampler (100) is including body (602) that has inner chamber (601), body (602) still have with the communicating opening of inner chamber (601), the lateral wall of inner chamber (601) is provided with sample portion (603), the sample collector stretch into the process of inner chamber (601) with sample portion (603) contact, sample portion (603) are taken out sample in the sample collector, blender (200) are including body (1) that has the cavity, the cavity with inner chamber (601) communicate with each other.

7. The sample and reagent mixing device of claim 6, wherein: the cavity still includes runner (5), inner chamber (601) pass through runner (5) with the cavity communicates with each other, the cavity includes premixing chamber (2), mixing chamber (4) and storage chamber (3), runner (5) through the oral area with premixing chamber (2) communicate with each other, at least some overlap settings of all oral areas.

8. The sample and reagent mixing device of claim 6, wherein: the mixing device further comprises a sample pushing device (300), wherein the sample pushing device (300) is arranged along the length direction of the body (1), all media in the flow channel (5) enter the premixing cavity (2), the mixing cavity (4) and the storage cavity (3) in sequence under the action of the sample pushing device (300).

9. The sample and reagent mixing device of claim 8, wherein: the sample pushing device (300) comprises a first part (400) and a second part, the body (1) is located between the first part (400) and the second part, the sample pushing device (300) further comprises a driver for driving the first part (400) and the second part to displace along the body (1), and when the driver drives the first part (400) and the second part to displace along a first direction, a medium located in the flow channel (5) sequentially enters the premixing cavity (2), the mixing cavity (4) and the storage cavity (3).

10. The sample and reagent mixing device of claim 9, wherein: the driver is a linear motor, or the driver is an electromagnet.