WO2022213630A1

WO2022213630A1 - Sample extractor and sample extraction method

Info

Publication number: WO2022213630A1
Application number: PCT/CN2021/134983
Authority: WO
Inventors: 丁子谦
Original assignee: 埃妥生物科技(杭州)有限公司
Priority date: 2021-04-08
Filing date: 2021-12-02
Publication date: 2022-10-13

Abstract

A sample extractor and a sample extraction method, which solve the technical problem in the prior art of difficult sample extraction operation. The sample extractor comprises a body (1), wherein the body (1) is provided with a sampling cavity (101) and a flow channel (102); the sampling cavity (101) is communicated with the flow channel (102); the body (1) is provided with a pressing area, and the pressing area is pressed to change the volume of the sampling cavity (101) to enable a medium in the sampling cavity (101) to enter the flow channel (102). During practical application, the sample collector is placed in the sampling cavity (101), a part of reagent can be pre-stored in the sampling cavity (101), and the sample collector is placed in the sampling cavity (101) to complete collection of the sample. The body (1) comprises the pressing area and the flow channel (102). By operating the pressing area to change the volume of the sampling cavity (101), it is convenient to extrude the medium in the sampling cavity (101) into the flow channel. The solution makes the sample extraction operation convenient and improves the operation efficiency of sample extraction.

Description

A sample extractor and method for extracting samples

technical field

The present disclosure relates to the technical field of sample extraction, and in particular, to a sample extractor and a method for extracting samples.

Background technique

Sample extraction refers to extracting the sample collected on the sample collector for processing in the next step. A commonly used sample collector includes a cotton swab. The cotton swab includes a rod body and a cotton swab head disposed at the end of the rod body. The cotton swab head contacts the human mucous membrane or body fluid to absorb the sample, and the sample is collected on the cotton swab head. Generally, the sample cannot be directly analyzed and measured. The sample needs to be dissolved in the reagent before measurement and analysis. The process of dissolving the sample on the cotton swab head in the reagent is the sample extraction.

In the prior art, test tube extraction is usually used for sample extraction. Specifically, a certain amount of reagent is stored in the test tube, and then the tip of the cotton swab is dipped into the reagent in the test tube to complete the sample extraction. After the sample extraction is completed, the sample in the test tube needs to be injected into the analytical instrument to complete the analysis and measurement. This operation method makes the sample extraction operation very inconvenient and reduces the sample extraction efficiency.

SUMMARY OF THE INVENTION

The present disclosure provides a sample extractor and a sample extraction method, which solve the technical problem of difficult sample extraction operations in the prior art.

In the first aspect, some implementations adopted to solve the above technical problems include:

A sample extractor includes a body, the body has a sampling cavity and a flow channel, wherein the sampling cavity is communicated with the flow channel, the body has a pressing area, and the sampling cavity is changed by pressing the pressing area The volume of the medium in the sampling chamber enters the flow channel.

In the practical application process, the sample collector is placed in the sampling chamber, and a part of the reagent can be pre-stored in the sampling chamber, and the sample collection is completed when the sample collector is placed in the sampling chamber. The body includes a pressing area and a flow channel. By operating the pressing area to change the volume of the sampling cavity, the medium in the sampling cavity can be easily squeezed into the flow channel. This solution makes the sample extraction operation very convenient and improves the operation efficiency of the sample extraction.

Preferably, the body further includes a reaction chamber, and the reaction chamber communicates with the sampling chamber through a flow channel.

In this solution, by setting the reaction chamber, the medium in the flow channel can directly enter the reaction chamber to complete the reaction, which optimizes the performance of the sample extractor. After the sample extractor extracts the sample, it can be directly analyzed or measured, which further optimizes the performance of the sample extractor.

Preferably, the body includes a diaphragm and an isolation bar disposed on the diaphragm, and the isolation bar isolates the space enclosed by the diaphragm to form the sampling chamber, the reaction chamber and the flow channel.

In this solution, the body forms the sampling cavity, the reaction cavity and the flow channel through the diaphragm and the spacer strip, the sample extractor is simple in structure and easy to process, and the use cost of the sample extractor is low.

Preferably, the body further includes a reagent cavity, the body is provided with a spacer, and the spacer isolates the space enclosed by the membrane to form the reagent cavity.

In this solution, the setting of the spacer forms a reagent chamber, and different reagents can be placed in different reagent chambers, which further optimizes the performance of the sample extractor. Different reagents can be stored in the reagent chamber, which broadens the application range of the sample extractor. .

Preferably, the reagent chamber communicates with the reaction chamber, and the flow channel, the reagent chamber and the reaction chamber are at least partially overlapped with each other.

In this scheme, the communication between the flow channel, the reagent chamber and the reaction chamber is at least partially overlapped, the medium entering the reaction chamber through the flow channel and the medium entering the reaction chamber with the reagent have a larger contact area, and the medium in the flow channel and the medium in the reagent chamber have a larger contact area. The medium has a large contact area in the reaction chamber, so that each medium in the reaction chamber is uniformly mixed under the action of molecular force, and the application performance of the sample extractor is optimized.

Preferably, the flow channel, the reagent chamber and the reaction chamber are overlapped with each other.

In this solution, the contact area between the medium in the flow channel and the medium in the reagent chamber in the reaction chamber is increased, the mixing performance of each medium in the reaction chamber is further optimized, and the use performance of the sample extractor is optimized.

Preferably, the body further includes a sealing body for sealing the sampling cavity.

In this solution, the setting of the sealing body mainly plays the function of sealing the sampling cavity, the medium in the sampling cavity is not easy to leak, and the sample extractor is easy to recover.

Preferably, the sealing body is a sealing cover arranged on the main body.

In this solution, the sealing cap has a better application experience, which optimizes the application performance of the sample extractor.

Preferably, the sealing body is a seal provided on the main body.

In this solution, the provision of the seal reduces the manufacturing cost of the sample extractor.

Preferably, the body is provided with a membrane valve, and the membrane valve is arranged in the flow channel to prevent the medium in the flow channel from flowing back to the sampling chamber.

In this solution, the setting of the membrane valve can prevent the medium in the flow channel from flowing back into the sampling chamber, and the sample extractor has better sample extraction capability.

Compared with the prior art, the sample extractor provided by the present disclosure has the following advantages:

1. By setting the pressing area, in the process of extracting the sample, the medium in the sampling cavity can be input into the flow channel only by operating the pressing area, thereby improving the sampling efficiency of the sample and making the sample extraction operation convenient.

2. The body includes a diaphragm and an isolation strip, and the sample extractor has a simple structure and is easy to process, which reduces the use cost of the sample extractor.

In the second aspect, some embodiments adopted to solve the above technical problems further include:

A sample extractor includes a main body, the main body has a sampling cavity, a flow channel and a reaction cavity, the sampling cavity is communicated with the reaction cavity through the flow channel, and the main body also has a reagent cavity, the reagent cavity is connected with the reaction cavity. The reaction chamber is communicated, and a one-way valve is arranged between the flow channel and the sampling chamber to prevent the medium in the flow channel from flowing back to the sampling chamber, wherein the body has a pressing area, and operating the pressing area makes all the The sampling chamber and the medium in the reagent chamber enter the reaction chamber at the same time.

In the actual application process, different reagents are preset in the sampling chamber and the reagent chamber, and the sample collector is placed in the sampling chamber. Then, the different media in the sampling chamber and the reagent chamber can enter the reaction by operating the pressing area. Intra-cavity reaction, this scheme makes the sample extraction operation very convenient and reduces the labor intensity of the operator.

Preferably, the main body is made of the same piece of soft film, wherein the main body is formed with spacer bars by hot pressing, and the spacer bars divide the cavity enclosed by the main body to form the sampling cavity, the The reaction chamber and the flow channel.

In this solution, the body is made of the same piece of soft film, and spacers are formed on the soft film by hot pressing, and the structure of the body is simple and easy to process.

In addition, the body is made of a soft film, and the entire body has a pressing ability, which is beneficial to the sample extraction operation. For example, when pressing the main body, any pressing method can be used to make the medium in the main body flow.

At the same time, because the body is made of a soft film, the space enclosed by the body is easy to form a vacuum, so as to prevent air from entering the space enclosed by the body, thereby avoiding air contamination of the sample. When the medium enters the reaction chamber, it is favorable for the medium to enter the sampling chamber completely, and the consumption of reagents is saved.

Preferably, the main body is provided with a spacer, the spacer is made of the same material as the main body, the spacer is arranged in the space enclosed by the main body, and the space enclosed by the main body is divided to form the Reagent chamber.

In this solution, the material of the spacer is the same as that of the body, and the spacer is easy to connect with the body, which reduces the processing cost of the sample extractor.

Preferably, the spacer is arranged in the space enclosed by the body by means of hot pressing, and an end of the reagent chamber away from the reaction chamber is provided with a mouth that communicates with the outside world.

In this solution, the spacer is connected to the body by hot pressing, and the spacer and the body are easy to process, which reduces the manufacturing cost of the sample extractor. The arrangement of the mouth can conveniently discharge the air in the reagent chamber, and then the reagent can be conveniently filled into the reagent chamber.

Preferably, the body is provided with a sealing clip for closing the mouth.

In this solution, the setting of the sealing clip can conveniently close the mouth and effectively avoid the leakage of the medium in the reagent chamber.

Preferably, the reagent chamber, the flow channel and the reaction chamber are at least partially overlapped with each other.

In this solution, the medium in the reagent chamber and the medium in the flow channel have a larger contact area when entering the reaction chamber, so that the medium in the reagent chamber and the medium in the flow channel are uniformly mixed.

Preferably, the flow channel includes a first part that communicates with the sampling chamber and a second part that communicates with the reaction chamber, the first part communicates with the second part, and the second part communicates with the reagent At least a portion of the cavities overlap.

In this solution, the arrangement of the sampling chamber, the flow channel and the reagent chamber is reasonable, which reduces the volume of the sample extractor, thereby widening the application range of the sample extractor.

Preferably, the first part is perpendicular to the second part.

In this solution, the arrangement of the sampling cavity and the flow channel is reasonable, which further reduces the volume of the sample extractor.

In a third aspect, the present disclosure provides a method for extracting samples, which solves the technical problem of low sample extraction efficiency in the prior art.

The technical solutions adopted to solve the above technical problems include:

A method for extracting samples, including a sample extractor, the sample extractor is the sample extractor disclosed in the first aspect, and the extraction method includes the following steps:

Presetting a first reagent in the sampling chamber;

Presetting a second reagent in the reagent chamber;

inserting a sample collector into the sampling chamber;

applying a first force to make the medium in the sampling chamber enter the flow channel;

Applying a second force causes the flow channel and the medium in the reagent chamber to enter the reaction chamber at the same time.

In the actual application process, the sample and the reagent can be injected into the reaction chamber for reaction by applying the force twice, which greatly improves the extraction efficiency of the sample and reduces the labor intensity of the operator. In addition, the medium in the reagent chamber and the medium in the flow channel enter the reaction chamber at the same time, which is conducive to the uniform mixing of the medium in the reagent chamber and the medium in the flow channel in the reaction chamber, and the uniform mixing of the medium in the reaction chamber is conducive to subsequent analysis and detection , which further improves the extraction efficiency of samples.

Preferably, when the medium in the reagent chamber and the medium in the flow channel enter the reaction chamber at the same time, the contact width between the medium in the reagent chamber and the medium in the flow channel is not less than the width of the flow channel. width.

In this scheme, the medium in the flow channel and the medium in the reagent chamber have a large contact area in the reaction chamber. Under the action of molecular force, the medium in the reaction chamber is mixed evenly, which improves the extraction efficiency of the sample.

Description of drawings

For purposes of explanation, several embodiments of the disclosed technology are set forth in the following figures. The following drawings are incorporated herein and constitute a part of the detailed description. In some instances, well-known structures and components are shown in block diagram form in order to avoid obscuring the concepts of the disclosed subject technology.

FIG. 1 is a front view of a first aspect of the present disclosure.

FIG. 2 is a cross-sectional view at A-A in FIG. 1 .

FIG. 3 is an enlarged view of B in FIG. 2 .

FIG. 4 is a schematic diagram of a first implementation manner of the first aspect of the disclosure.

FIG. 5 is a schematic diagram of a second embodiment of the first aspect of the present disclosure.

FIG. 6 is a schematic diagram of a third implementation manner of the first aspect of the present disclosure.

7 is a front view of the second aspect of the present disclosure.

FIG. 8 is a cross-sectional view at C-C in FIG. 7 .

FIG. 9 is an enlarged view of D in FIG. 8 .

10 is an isometric view of the second aspect of the present disclosure.

Shown in the picture:

1. Main body, 101, sampling chamber, 102, flow channel, 103, reaction chamber, 104, diaphragm, 105, spacer strip, 106, reagent chamber, 2, spacer, 31, sealing cover, 32, seal, 4, membrane valve.

1a, body, 101a, sampling chamber, 3, flow channel, 301, first part, 302, second part, 103a, reaction chamber, 5, reagent chamber, 6, one-way valve, 7, spacer bar, 8, spacer , 9, sealing clip.

Detailed ways

The specific embodiments shown below are intended as descriptions of various configurations of the disclosed subject technology and are not intended to represent the only configurations in which the disclosed subject technology may be practiced. The specific embodiments include specific details for the purpose of providing a thorough understanding of the subject technology of the present disclosure. However, it will be apparent and apparent to those skilled in the art that the disclosed subject technology is not limited to the specific details shown herein and may be practiced without these specific details.

1 to 6 are schematic structural diagrams of the sample extractor according to the first aspect of the disclosure.

Referring to FIG. 1 , FIG. 2 and FIG. 3 , a sample extractor includes a main body 1 , and the main body 1 has a sampling cavity 101 and a flow channel 102 , wherein the sampling cavity 101 communicates with the flow channel 102 , The body 1 has a pressing area, and the pressing area is pressed to change the volume of the sampling cavity 101 so that the medium in the sampling cavity 101 enters the flow channel 102 .

During actual use, a certain amount of reagents are stored in the sampling chamber 101, therefore, the sampling chamber 101 should have a certain sealing performance. Specifically, a certain amount of reagent is put into the sampling cavity 101 first, and then a sample collector is put into the sampling cavity 101. The sample collector takes a cotton swab as an example. The reagents will enter the sampling chamber 101 to achieve sample extraction.

Sample extraction is only one of the operation procedures. To analyze and detect the sample, the medium in the sampling chamber 101 needs to be placed in the relevant instrument. Therefore, the medium in the sampling chamber 101 should be easy to take out. The present disclosure takes out the medium in the sampling chamber 101 by setting the flow channel 102 .

When the sampling cavity 101 has sealing properties, the volume of the sampling cavity 101 is changed by operating the pressing part, at this time, the medium in the sampling cavity 101 is squeezed into the flow channel 102, which is beneficial to take out the medium in the sampling cavity 101. Therefore, the sample extraction operation is convenient, and the operation efficiency of the sample extraction is improved.

Usually, the medium in the flow channel 102 needs to be placed in the reaction chamber 103 with reaction capability, and the medium in the reaction chamber 103 can be directly analyzed or measured by the instrument.

Referring to FIG. 1 to FIG. 3 , in some embodiments, the body 1 further includes a reaction chamber 103 , and the reaction chamber 103 communicates with the sampling chamber 101 through a flow channel 102 .

The main body 1 includes a diaphragm 104 and a spacer 105 disposed on the diaphragm 104 . The spacer 105 isolates the space enclosed by the diaphragm 104 to form the sampling chamber 101 , the reaction chamber 103 , and the space. The flow channel 102 is described.

The body 1 further includes a reagent cavity 106 , and the body 1 is provided with a spacer 2 , and the spacer 2 isolates the space enclosed by the membrane 104 to form the reagent cavity 106 .

In this embodiment, the diaphragm 104 is a thin film in the prior art, and its preparation process refers to the prior art. It can be understood that the material of the diaphragm 104 should not react chemically with the reagent and the sample, so as to avoid the diaphragm 104 Contaminated samples.

The diaphragm 104 has soft properties, so the pressing area may refer to the part of the diaphragm 104 that surrounds the sampling cavity 101 . That is, the side wall of the sampling cavity 101 is soft, and the volume of the sampling cavity 101 can be changed by pressing operation.

Another characteristic of the diaphragm 104 is that it is suitable for processing. For example, various shapes of closed areas can be formed on the diaphragm 104 by heat sealing or hot pressing. For example, the spacer bars 105 in this embodiment can be made by hot pressing. Specifically, when the diaphragm 104 forms the sampling chamber 101, the reaction chamber 103 and the flow channel 102, the diaphragm 104 needs to be folded to form a space between the diaphragms 104, and then a part of the diaphragm 104 is melted by hot pressing. The spacer bar 105 is formed after the part of the cooling element is cooled.

The sample extractor of this embodiment is easy to manufacture and has a low cost. Generally, the body 1 is provided with a membrane valve 4 , and the membrane valve 4 is provided in the flow channel 102 to prevent the medium in the flow channel 102 from flowing back to the sampling chamber 101 . The main function of the membrane valve 4 is to prevent the medium in the flow channel 102 from flowing back into the sampling chamber 101 . Specifically, when the medium in the flow channel 102 is pressed into the sampling chamber 101 or during other operations on the sample extractor, the flow channel 102 or the reaction chamber 103 may be squeezed, because the sample extractor is made of the diaphragm 104 , at this time, the medium in the flow channel 102 may return to the sampling chamber 101 . In order to prevent the medium in the flow channel 102 from entering the sampling chamber 101 by mistake, the backflow of the medium in the flow channel 102 can be effectively avoided by setting the membrane valve 4 .

The membrane valve 4 is a one-way valve made of a membrane. The one-way valve has the ability of one-way conduction, and the volume is small. Specifically, the membrane valve 4 includes two membranes, a valve cavity is formed between the two membranes, the sampling cavity 101 communicates with the valve cavity, the flow channel 102 communicates with the valve cavity, and the medium in the flow channel 102 will also distribute On the outside of the two films, when the flow channel 102 is squeezed, the medium in the flow channel 102 will have a squeezing effect on the two films, and at this time, the valve cavity is closed. Therefore, the membrane valve 4 has a one-way conduction capability.

Usually, various reagents may be required to react and mix in the reaction chamber 103 during various analysis and detection processes. Therefore, by setting the spacer 2 to isolate the reagent chamber 106, different reagents can be preset in the sample extractor. The spacer 2 can also be disposed on the body 1 through a hot pressing process. Specifically, the material of the diaphragm 104 may be the same as that of the body 1 . After the separator 2 is placed at the target position, the membrane 104 and a part of the separator 2 are melted by hot pressing, and the reagent chamber 106 is formed after the part is cooled.

The greater the number of spacers 2 , the more reagent chambers 106 are formed. The specific number of spacers 2 is not limited, and should be reasonably configured according to the types of reagents in the actual design process.

In some embodiments, the reagent chamber 106 communicates with the reaction chamber 103 , and the flow channel 102 , the reagent chamber 106 and the reaction chamber 103 at least partially overlap.

The flow channel 102 , the reagent chamber 106 and the reaction chamber 103 are overlapped with each other.

Referring to Figures 2 and 3, when the medium is in a state of micron thickness, various media can be mixed with each other under the action of molecular force, and the medium in the reaction chamber 103 of the sample extractor should be mixed evenly to improve detection and analysis accuracy. . Therefore, the medium in the flow channel 102 and the medium in the reagent chamber 106 should be fully contacted when entering the reaction chamber 103 to improve the uniformity of the medium in the reaction chamber 103 . Therefore, the medium in the flow channel 102 and the medium in the reaction chamber 103 should have as large a contact area as possible in the reaction chamber 103 , so that different media can be uniformly mixed in the reaction chamber 103 .

In this embodiment, the flow channel 102, the reagent chamber 106 and the reaction chamber 103 are partially or completely overlapped. The medium in the flow channel 102 and the reagent chamber 106 has a large contact area when entering the reaction chamber 103, and the mixing is uniform, which optimizes the sample extractor. application performance.

Referring to FIG. 4 to FIG. 6 , in some embodiments, the body 1 further includes a sealing body for sealing the sampling cavity 101 .

In the above description, the sampling cavity 101 should have certain sealing characteristics to avoid leakage of the medium in the sampling cavity 101. However, the sampling cavity 101 should have an opening so that the cotton swab can be placed in the sampling cavity 101. Therefore, the main body 1 should also be provided with A sealing body to open or close the sampling chamber 101 .

Referring to FIG. 4 and FIG. 5 , the sealing body is a sealing cover 31 disposed on the main body 1 . The sealing cover 31 is a structure externally placed on the body 1 . Specifically, the sealing cover 31 may include a mouth and a cover, the mouth is placed on the body 1 , and the cover can be screwed on the mouth to achieve sealing. This solution can meet the functional requirements of the sealing body.

Referring to FIG. 6 , another embodiment of the sealing body is: the sealing body is a seal 32 provided on the main body 1 . The seal 32 is a structure formed on the body 1, and this solution does not require an external structure. Specifically, the sampling cavity 101 has an opening. After the cotton swab is placed in the sampling cavity 101 , the membranes 104 on both sides of the opening are closed by hot pressing to form the seal 32 .

For example, when the seal 32 is used as the sealing body, a structure such as a heat sealer can be built into the instrument so that the seal 32 is formed in the instrument. This solution will make the sample extractor have better operational performance.

The technical solution provided by the present disclosure can be applied to any sample extraction equipment, for example, a fluorescence quantitative PCR instrument and other instruments.

The technical solutions and corresponding details of the subject matter of the present disclosure have been introduced above. It should be understood that the above descriptions are only some embodiments of the technical solutions of the subject matter of the present disclosure, and some details may be omitted during specific implementation.

In addition, in some embodiments disclosed above, it is possible to implement a plurality of embodiments in combination, and various combination solutions are not listed one by one due to space limitations. Those skilled in the art can freely combine and implement the above embodiments according to requirements during specific implementation, so as to obtain a better application experience.

7 to 10 are schematic structural diagrams of the sample extractor according to the second aspect of the disclosure.

7 to 10, a sample extractor includes a body 1a, the body 1a has a sampling cavity 101a, a flow channel 3 and a reaction cavity 103a, the sampling cavity 101a is connected to the reaction cavity through the flow channel 3 103a communicates with each other, the main body 1a also has a reagent chamber 5, the reagent chamber 5 communicates with the reaction chamber 103a, and between the flow channel 3 and the sampling chamber 101a is provided to prevent the medium in the flow channel 3 from flowing back into the chamber. In the one-way valve 6 of the sampling chamber 101a, the body 1a has a pressing area, and operating the pressing area causes the sampling chamber 101a and the medium in the reagent chamber 5 to enter the reaction chamber 103a at the same time.

In the actual use process, a certain amount of reagent is stored in the sampling chamber 101a, and a certain amount of reagent should also be preset in the reagent chamber 5. The preset reagent in the reagent chamber 5 is different from that in the sampling chamber 101a. reagents. Usually, the subsequent analysis, detection, etc. of the sample needs to be mixed with different reagents. Therefore, in order to improve the extraction efficiency of the sample, in addition to quickly taking the sample out of the sample collector, it is also necessary to quickly mix the sample with different reagents and shorten the waiting time for analysis and detection.

Since the sampling chamber 101a and the reagent chamber 5 are preset with different reagents, the sampling chamber 101a and the reagent chamber 5 should be easily sealed during the specific operation to avoid sample contamination. Specifically, after the sample collector is placed in the sampling chamber 101a, the reagent chamber 5 should be sealed.

During the sample extraction operation, the medium in the flow channel 3 and the reagent chamber 5 can be squeezed into the reaction chamber 103a by operating the pressing area, that is, the first force is applied to make the medium in the sampling chamber 101a enter the flow channel 103a. Channel 3, applying a second force to make the flow channel 3 and the medium in the reagent chamber 5 enter the reaction chamber 103a at the same time. The medium in the reagent chamber 5 and the medium in the flow channel 3 enter the reaction chamber 103a at the same time, so that the medium in the flow channel 3 and the medium in the reagent chamber 5 can be uniformly mixed in the reaction chamber 103a, and the mixing is under the molecular force. the mix of. When the medium has a thickness of micrometers, the mixing of two different mediums can be realized under the molecular force.

Through the above introduction, the technical solution provided by the present disclosure greatly improves the efficiency of sample extraction and reduces the labor intensity of operators. In addition, the medium in the flow channel 3 and the reagent chamber 5 can be completely entered into the reaction chamber 103a, which reduces the labor intensity of the operator while saving costs.

The one-way valve 6 has the capability of one-way conduction and is small in size. Specifically, the one-way valve 6 can be a membrane valve, wherein the membrane valve includes two thin films, a valve cavity is formed between the two thin films, the sampling cavity 101a communicates with the valve cavity, and the flow channel 3 communicates with the valve cavity at the same time. The medium in the flow channel 3 will also be distributed on the outside of the two films. When the flow channel 3 is squeezed, the medium in the flow channel 3 will have a squeezing effect on the two films. At this time, the valve cavity is closed. Therefore, the membrane valve has the ability to conduct one-way conduction.

The one-way valve 6 can also be replaced by other structures with one-way conduction capability. However, considering the volume of the main body 1a and the manufacturing cost, the one-way valve 6 is preferably a membrane valve with a simple structure and low cost.

It can be understood that the one-way valve 6 can be not only the solid valve described in the above embodiments, but also a non-solid valve, that is, the behavior of not withdrawing the first force also constitutes the essence of the one-way valve.

In some embodiments, the body 1a is made of the same piece of soft film, wherein the body 1a is formed with spacer bars 7 by hot pressing, and the spacer bars 7 divide the cavity enclosed by the body 1a The sampling chamber 101a, the reaction chamber 103a and the flow channel 3 are formed.

The main body 1a is provided with a spacer 8, the material of the spacer 8 is the same as that of the main body 1a, the spacer 8 is arranged in the space enclosed by the main body 1a, and divides the space enclosed by the main body 1a. The space forms the reagent chamber 5 .

The spacer 8 is disposed in the space enclosed by the main body 1a by hot pressing, and an end of the reagent chamber 5 away from the reaction chamber 103a is provided with a mouth that communicates with the outside world.

The flexible membrane can be made of any material that does not chemically react with the reagent. In the actual production process, the soft film is first folded in half to form an open space, then the spacer 8 is placed in the open space, and finally, the opening of the open space is closed by the hot pressing process The body 1a can be formed there. Specifically, the hot pressing process melts or partially melts the soft film, and then the melted soft film is cooled to form corresponding spaces, and the spaces are separated from each other. For example, a flow channel 3, a reagent chamber 5, a reaction chamber 103a, a sampling chamber 101a and the like are formed. The process parameters of the hot pressing process are different according to different soft films, and are not limited in detail here. The body can be made of transparent sheet material, therefore, the structures such as the isolation bars in the drawings are replaced by lines.

In some embodiments, the body 1a is provided with a sealing clip 9 closing the mouth.

The reagent chamber 5, the flow channel 3 and the reaction chamber 103a are at least partially overlapped with each other.

The flow channel 3 includes a first part 301 that communicates with the sampling chamber 101a and a second part 302 that communicates with the reaction chamber 103a. The first part 301 communicates with the second part 302, and the second part 302 is disposed overlapping at least a part of the reagent chamber 5 .

The first part 301 is perpendicular to the second part 302 .

The sealing clip 9 can be a common clip in the prior art. Since the plastic clip has the advantages of low cost and long service life, the sealing clip 9 can preferably be a plastic clip. In addition, plastic clips are not prone to chemical reactions in the medium, which can avoid sample contamination.

In the actual application process, the equipment for analyzing and detecting samples has limited space for placing samples. Therefore, the volume of the main body 1a should not be too large. Generally, the volume of the main body 1a should be minimized in the design to realize the small size of the analysis and detection equipment. change.

Referring to FIG. 9 , in this embodiment, the volume of the main body 1 a is effectively reduced by rationally arranging the flow channel 3 and the reagent chamber 5 . In addition, the places where the reagent chamber 5, the flow channel 3 and the reaction chamber 103a communicate with each other are arranged at least partially overlapping, which increases the contact area between the medium in the reagent chamber 5 and the medium in the flow channel 3 in the reaction chamber 103a, so that the inside of the flow channel 3 is increased. The medium in the reaction chamber 103a is easy to mix with the medium in the reaction chamber 103a, which further improves the sample extraction efficiency.

In the above description, the sampling chamber 101a should have certain sealing properties to avoid leakage of the medium in the sampling chamber 101a. However, the sampling chamber 101a should have an opening so that the sample collector can be placed in the sampling chamber 101a. Therefore, the main body 1a also has an opening. A sealing body should be provided to open or close the sampling chamber 101a.

The sealing body is a sealing cover disposed on the body 1a. The sealing cover is a structure externally placed on the body 1a. Specifically, the sealing cover may include a mouth portion and a cover body, the mouth portion is placed on the body 1a, and the cover body can be screwed on the mouth portion to achieve sealing. This solution can meet the functional requirements of the sealing body.

Another embodiment of the sealing body is: the sealing body is a seal provided on the main body 1a. The structure formed on the seal body 1a does not require an external structure. Specifically, the sampling cavity 101a is provided with an opening. After the cotton swab is placed in the sampling cavity 101a, the membranes on both sides of the opening are closed by hot pressing to form a seal.

For example, when a seal is used as the sealing body, a structure such as a heat sealer can be built into the instrument, so that the seal is formed in the instrument, and this solution will enable the sample extractor to have better operational performance.

In a third aspect, a method for extracting a sample includes a sample extractor, the sample extractor is the sample extractor described in the first aspect, and the extraction method includes the following steps:

Presetting a first reagent in the sampling chamber 101a;

The second reagent is preset in the reagent chamber 5, and the reagent chamber 5 should be closed after the second reagent is preset in the reagent chamber 5 to avoid leakage of the second reagent in the reagent chamber 5;

A sample collector is inserted into the sampling cavity 101a. After the sample collector is placed in the sampling cavity 101a, the sampling cavity 101a should be closed to avoid leakage of the first medium in the sampling cavity 101a. The sampling cavity 101a is usually closed by heat sealing. , for the heat sealing method, refer to the introduction about the sealing body in the first aspect;

applying a first force to make the medium in the sampling chamber 101a enter the flow channel 3;

The second force is applied to cause the flow channel 3 and the medium in the reagent chamber 5 to enter the reaction chamber 103a at the same time.

Usually, the first acting force and the second acting force can be applied manually, or can be applied automatically by a force applying mechanism. When the first acting force and the second acting force are applied by means of automatic force application, the force applying mechanism may be a structure that outputs linear motion, such as an electromagnet or a linear motor. The first acting force may be formed by an electromagnet, that is, the electromagnet absorbs the armature and squeezes the body 1a, so that the medium in the sampling cavity 101a enters the flow channel 3 . The second acting force can use a linear motor as a power source to squeeze the medium in the flow channel 3 and the reagent chamber 5 into the reaction chamber 103a.

In some embodiments, when the medium in the reagent chamber 5 and the medium in the flow channel 3 enter the reaction chamber 103a simultaneously, the medium in the reagent chamber 5 and the medium in the flow channel 3 The contact width is not less than the width of the flow channel 3 .

In the technical solution of the present disclosure, the main body 1a is made of a soft film. Since the soft film has soft characteristics, in addition, the amount of reagent preset in the reagent chamber 5 is small. The medium in 5 will not automatically enter the reaction chamber 103a. Therefore, the medium in the flow channel 3 and the reagent chamber 5 needs to enter the reaction chamber 103a at the same time by applying the second force.

When those skilled in the art implement the subject technical solutions of the present disclosure, they can obtain other detailed configurations or drawings according to the subject technical solutions of the present disclosure and the accompanying drawings. The details still fall within the scope of the technical solutions of the presently disclosed subject matter.

Claims

A sample extractor, characterized by comprising a body (1), the body (1) having a sampling cavity (101) and a flow channel (102), wherein the sampling cavity (101) and the flow channel ( 102) are communicated, the body (1) has a pressing area, and the pressing area is pressed to change the volume of the sampling cavity (101) so that the medium in the sampling cavity (101) enters the flow channel (102).
The sample extractor according to claim 1, characterized in that: the body (1) further comprises a reaction chamber (103), and the reaction chamber (103) communicates with the sampling chamber (101) through a flow channel (102) Connected.
The sample extractor according to claim 2, characterized in that: the body (1) comprises a diaphragm (104) and a spacer (105) arranged on the film (104), the spacer (105) ) isolates the space enclosed by the diaphragm (104) to form the sampling chamber (101), the reaction chamber (103) and the flow channel (102).
The sample extractor according to claim 3, characterized in that: the main body (1) further comprises a reagent chamber (106), the main body (1) is provided with a spacer (2), and the spacer (2) The space enclosed by the diaphragm (104) is isolated to form the reagent chamber (106).
The sample extractor according to claim 4, characterized in that: the reagent chamber (106) communicates with the reaction chamber (103), and the flow channel (102) and the reagent chamber (106) communicate with the Where the reaction chambers (103) communicate with each other, they are arranged at least partially overlapping.
The sample extractor according to claim 5, wherein the flow channel (102), the reagent chamber (106) and the reaction chamber (103) communicate with each other and are arranged overlappingly.
The sample extractor according to any one of claims 1 to 6, characterized in that: the body (1) further comprises a sealing body for sealing the sampling cavity (101).
The sample extractor according to claim 7, wherein the sealing body is a sealing cover (31) provided on the main body (1).
The sample extractor according to claim 7, wherein the sealing body is a seal (32) provided on the main body (1).
The sample extractor according to claim 1, characterized in that: the body (1) is provided with a membrane valve (4), and the membrane valve (4) is arranged in the flow channel (102) to prevent the The medium in the flow channel (102) is returned to the sampling chamber (101).
A sample extractor, characterized by comprising a body (1a), the body (1a) having a sampling cavity (101a), a flow channel (3) and a reaction cavity (103a), the sampling cavity (101a) passing through the flow The channel (3) communicates with the reaction chamber (103a), the main body (1a) also has a reagent chamber (5), the reagent chamber (5) communicates with the reaction chamber (103a), and the flow channel ( 3) A one-way valve (6) for preventing the medium in the flow channel (3) from flowing back to the sampling chamber (101a) is provided between the sampling chamber (101a), wherein the main body (1a) has a pressing area, operating the pressing area to make the sampling chamber (101a) and the medium in the reagent chamber (5) enter the reaction chamber (103a) at the same time.
The sample extractor according to claim 11, characterized in that: the main body (1a) is made of the same soft film, wherein the main body (1a) is formed with spacers (7) by hot pressing, The spacer strip (7) divides the cavity enclosed by the body (1a) to form the sampling cavity (101a), the reaction cavity (103a) and the flow channel (3).
The sample extractor according to claim 12, characterized in that: the main body (1a) is provided with a spacer (8), the spacer (8) is made of the same material as the main body (1a), and the spacer (1a) is made of the same material. The sheet (8) is arranged in the space enclosed by the main body (1a), and divides the space enclosed by the main body (1a) to form the reagent cavity (5).
The sample extractor according to claim 13, characterized in that: the spacer (8) is arranged in the space enclosed by the main body (1a) by hot pressing, and the reagent chamber (5) is far away from the One end of the reaction chamber (103a) is provided with a mouth communicating with the outside.
The sample extractor according to claim 14, wherein the main body (1a) is provided with a sealing clip (9) for closing the mouth.
The sample extractor according to any one of claims 11 to 15, characterized in that the reagent chamber (5), the flow channel (3) and the reaction chamber (103a) are at least partially overlapped with each other.
The sample extractor according to claim 16, characterized in that: the flow channel (3) comprises a first part (301) communicating with the sampling chamber (101a) and a first part (301) communicating with the reaction chamber (103a) Two parts (302), the first part (301) communicates with the second part (302), and the second part (302) and the reagent chamber (5) at least partially overlap.
The sample extractor according to claim 17, wherein the first part (301) and the second part (302) are arranged vertically.
A method for extracting a sample, comprising a sample extractor, characterized in that: the sample extractor is the sample extractor described in any one of claims 11 to 18, and the extraction method comprises the following steps:

Presetting a first reagent in the sampling chamber (101a);

Presetting a second reagent in the reagent chamber (5);

inserting a sample collector into the sampling chamber (101a);

applying a first force to make the medium in the sampling chamber (101a) enter the flow channel (3);

A second force is applied to cause the flow channel (3) and the medium in the reagent chamber (5) to enter the reaction chamber (103a) at the same time.
The method for extracting a sample according to claim 19, characterized in that: when the medium in the reagent chamber (5) and the medium in the flow channel (3) enter the reaction chamber (103a) at the same time, the The contact width between the medium in the reagent chamber (5) and the medium in the flow channel (3) is not less than the width of the flow channel (3).