CN207143217U - Digital pcr amplification instrument - Google Patents
Digital pcr amplification instrument Download PDFInfo
- Publication number
- CN207143217U CN207143217U CN201720973020.6U CN201720973020U CN207143217U CN 207143217 U CN207143217 U CN 207143217U CN 201720973020 U CN201720973020 U CN 201720973020U CN 207143217 U CN207143217 U CN 207143217U
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- China
- Prior art keywords
- digital pcr
- cover plate
- sample
- pcr amplification
- amplification instrument
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Abstract
Biochemistry experiment instrument is the utility model is related to, specially a kind of digital pcr amplification instrument, the digital pcr amplification instrument is adapted in use to array digital pcr chip；The digital pcr amplification instrument includes：Automatic sample feeding device, realize that automatic sample operates to array digital pcr chip.The digital pcr amplification instrument of utility model is realized using array digital pcr chip to be treated sample measuring liquid body while is loaded onto in each PCR reaction tanks, avoid producing nonspecific reaction, specifically pass through substrate and cover plate compression fit, cover plate is slided under the cooperation of pressing device and pushing meanss, and then make the liquid in each liquid storage chamber while enter in corresponding PCR reaction tanks, mixed with primer and probe in reaction tank, avoid producing nonspecific reaction, improve the accuracy of subsequent detection data.
Description
Technical field
It the utility model is related to biochemistry experiment instrument, and in particular to a kind of array digital pcr chip.
Background technology
PCR（PCR）It has been widely used in medical science, science of heredity, microbiology or even whole life science
In.Real-time quantitative PCR array technique is using Real-time quantitative PCR as core, and combines array technique, realizes single experiment
A kind of new amplification technique of detectable series bulk gene, the technology being capable of reliable, scale detection signals exactly
The related gene expression profile of the important biomolecule processes such as transduction, disease related pathways, in microorganism, hereditary disease, tumour, drug gene
There is important application in the subjects such as group.
In the prior art, digital polymerase chain reaction is performed using base material design chips（Digital PCR）.Pass through two
The overlapping loading of the individual glass slide with pore passage structure, merging two sides duct turns into a complete runner, and in the substrate
Provided with the how individual reaction tanks of N, runner will treat that sample measuring liquid body is sequentially added in each reaction tank, and this time difference will shift to an earlier date partial reaction pond
Sample measuring liquid body is treated in acquisition, is mixed with primer and probe in reaction tank, produces nonspecific reaction, rather than specific PCR production
Thing will produce interference to testing result；And the primer and probe material in the reaction tank of runner front portion is easy due to liquid flowing impulse force
It is brought on a small quantity in next reaction tank, more primed probe mixing, produces multi-products in one reaction pool, cause result can not
Interpretation；And there has been no a digital pcr amplification instrument that can promote to treat sample measuring liquid body while be added to each PCR reaction tanks.
Utility model content
The purpose of this utility model is to provide a kind of digital pcr amplification instrument, and it is anti-to treat that sample measuring liquid body is loaded onto each PCR with guarantee
Pond while property is answered, avoids producing nonspecific reaction.
In order to solve the above-mentioned technical problem, the utility model provides a kind of digital pcr amplification instrument, and the digital pcr expands
Increase instrument and be adapted in use to array digital pcr chip；The digital pcr amplification instrument includes：Automatic sample feeding device, to array numeral
Pcr chip realizes that automatic sample operates.
Further, the array digital pcr chip includes substrate and cover plate, wherein
The upper surface of the substrate is placed with some chutes；The bottom surface medial recess of each chute forms a PCR reaction tanks；
The underside view of part of the cover plate has some sliding block projections for being suitable to respectively engage with each chute；
After cover plate is placed on substrate, chute corresponding to the raised engaging of sliding block, now, vacated between sliding block projection and chute
One liquid storage chamber；
Offer fluid channel on the chute wall of liquid storage chamber both sides, and by the fluid channel by each liquid
Storage chamber is connected；
The substrate is additionally provided with total runner, and liquid storage chamber is entered after sample measuring liquid body first passes through total runner, then by each micro-
Runner sequentially enters respective liquid storage chamber.
Well is offered on the cover plate, the perforate mouth of the well is directed at the head end of total runner；
Negative pressure hole is further opened with the cover plate, the perforate mouth of the negative pressure hole is directed at the end of total runner；
The automatic sample feeding device includes：The sample-adding mouth of sample-adding operation is realized for inserting well；And insertion negative pressure
Hole carries out the negative pressure mouth of negative-pressure operation to liquid storage chamber；
In sample-adding, treat that sample measuring liquid body is added in array digital pcr chip from sample-adding mouth, and enter by negative pressure mouth
Row extracts, so that test sample liquid is full of in each liquid storage chamber.
Further, the digital pcr amplification instrument also includes：Pressing device and pushing meanss；
Before sample-adding, the pressing device, which is suitable to compress substrate with cover plate, to be bonded；And
After sample-adding, the pushing mechanism is suitable to promote cover plate to slide, to form runner of releasing in sliding block high spot,
So as to treat that sample measuring liquid body flows into PCR reaction tanks by runner of accordingly releasing in each liquid storage chamber.
Further, the pressing device includes：Elevating mechanism, and two press strips consistent with cover plate glide direction, and two
Press strip is suitable to the upper surface both sides for pushing down cover plate respectively；And
Cushion is provided with positioned at the lower surface of press strip.
Further, the pushing meanss are located at the rear side of cover plate glide direction, to promote cover plate keeping compressing with substrate
Slided during state.
The beneficial effects of the utility model are that digital pcr amplification instrument of the present utility model uses array digital pcr chip
Realization is treated sample measuring liquid body while is loaded onto in each PCR reaction tanks, avoids producing nonspecific reaction, specifically passes through substrate and lid
Plate compression fit, cover plate is slided under the cooperation of pressing device and pushing meanss, and then make the liquid in each liquid storage chamber
Enter simultaneously in corresponding PCR reaction tanks, mixed with primer and probe in reaction tank, avoid producing nonspecific reaction, carry
The accuracy of high subsequent detection data.
Brief description of the drawings
The utility model is further illustrated with reference to the accompanying drawings and examples.
Fig. 1（a）It is the structural representation of array digital pcr chip of the present utility model
Fig. 1（b）It is the structural representation and partial enlarged drawing of substrate of the present utility model；
Fig. 2（a）It is the structural representation of chute corresponding to the raised engaging of sliding block of the present utility model；
Fig. 2（b）It is Fig. 2（a）The profile of middle A-A positions；
The sliding block raised schematic diagram that runner of releasing is formed with chute when Fig. 3 is slide cover；
Fig. 4（a）It is the sliding block projection and chute fit structure schematic diagram after the extruding of liquid storage chamber disappears；
Fig. 4（b）It is Fig. 4（a）The profile of middle B-B positions；
Fig. 5 is pressing device and the working state schematic representation of pushing meanss in digital pcr amplification instrument.
In figure：
Substrate 1, total runner 100, the head end 100a of total runner, chute 101, PCR reaction tanks 102, fluid channel 103；
Cover plate 2, well 200a, negative pressure hole 200b, sliding block projection 201, discharging slot 202, sliding block raised front portion 201a, cunning
Block rear raised portion 201b；
Liquid storage chamber 3；
Pressing device 4, cushion 401,
Pushing meanss 5.
Embodiment
The utility model is described in further detail presently in connection with accompanying drawing.These accompanying drawings are simplified schematic diagram,
Only illustrate basic structure of the present utility model in a schematic way, therefore it only shows the composition relevant with the utility model.
In the prior art, digital polymerase chain reaction is performed using base material design chips（Digital PCR）.Pass through two
The overlapping loading of the individual glass slide with pore passage structure, merging two sides duct turns into a complete runner, and in the substrate
Provided with the how individual reaction tanks of N, runner will treat that sample measuring liquid body is sequentially added in each reaction tank, and this time difference will shift to an earlier date partial reaction pond
Sample measuring liquid body is treated in acquisition, is mixed with primer and probe in reaction tank, produces nonspecific reaction, and nonspecific products will
Interference is produced to testing result；And the primer and probe material in the reaction tank of runner front portion is easily a small amount of due to liquid flowing impulse force
It is brought into next reaction tank, more primed probe mixing, produces multi-products in one reaction pool, cause result not sentence
Read；And there is not a digital pcr amplification instrument that can promote to treat sample measuring liquid body while be added to each PCR reaction tanks yet.
In order to solve the above-mentioned technical problem, this array digital pcr chip, digital pcr are expanded by following examples
Instrument is described in detail.
Fig. 1（a）It is the structural representation of array digital pcr chip of the present utility model
Fig. 1（b）It is the structural representation and partial enlarged drawing of substrate 1 of the present utility model；
Fig. 2（a）It is the structural representation of chute 101 corresponding to the engaging of sliding block projection 201 of the present utility model；
Fig. 2（b）It is Fig. 2（a）The profile of middle A-A positions；
Refer to Fig. 1（a）To Fig. 2（b）Shown, the present embodiment 1 provides a kind of array digital pcr chip, including：
Substrate 1 and cover plate 2, wherein
The upper surface of the substrate 1 is placed with some chutes 101；It is anti-that the bottom surface medial recess of each chute 101 forms a PCR
Answer pond 102；The underside view of part of the cover plate 2 has some sliding block projections 201 suitable for engaging respectively with each chute 101；Work as lid
After plate 2 is placed on substrate 1, chute 101 corresponding to the engaging of sliding block projection 201 is now, empty between sliding block projection 201 and chute 101
Go out a liquid storage chamber 3；It is raised in sliding block by slide cover 2 when after each liquid storage chamber 3 is each filled with after sample measuring liquid body
Runner of releasing is formed at 201, so as to treat that sample measuring liquid body flows into PCR reaction tanks 102 by runner of accordingly releasing.
This array digital pcr chip is coordinated by substrate 1 and cover plate 2, in the promotion lower cover 2 of digital pcr amplification instrument
Slide, make the liquid in each liquid storage chamber 3 while enter in corresponding PCR reaction tanks 102, enter with primer and probe in reaction tank
Row mixing, avoid producing nonspecific reaction, improve the accuracy of subsequent detection data.
Refer to Fig. 1（b）, fluid channel 103 is offered in the side wall of chute 101 of the both sides of liquid storage chamber 3, and lead to
Cross the fluid channel 103 each liquid storage chamber 3 is connected；The substrate 1 is additionally provided with total runner 100, treats that sample measuring liquid body first passes through
Enter liquid storage chamber 3 after total runner 100, then respective liquid storage chamber 3 is sequentially entered by each fluid channel 103.
The perforate mouth that well 200a, the well 200a are offered on the cover plate 2 is directed at the head end of total runner
100a（It is indicated in Figure 5）；
The perforate mouth that negative pressure hole 200b, the negative pressure hole 200b are further opened with the cover plate 2 is directed at the end of total runner 100
End.
Wherein, well 200a such as, but not limited to uses horn mouth, and big mouth is set outwardly.
Sliding block projection 201 forms the schematic diagram for runner of releasing with chute 101 when Fig. 3 is slide cover 2；
Fig. 4（a）It is the sliding block projection 201 and the fit structure schematic diagram of chute 101 after the extruding of liquid storage chamber 3 disappears；
Fig. 4（b）It is Fig. 4（a）The profile of middle B-B positions.
Refer to Fig. 2（a）To Fig. 4（b）, the chute 101 is cuboid, and the fluid channel 103 is located at chute 101
In long side wall, 201 cuboid to be fastened with the chute 101 of the sliding block projection；The bottom surface of the sliding block projection 201
It is in contact with the bottom surface of chute 101；A discharging slot 202 is opened up on the bottom surface of the sliding block projection 201, sliding block is raised
201 points are front and rear；Raised 201 engagement directions of the direction of the discharging slot 202 and sliding block are orthogonal, and before making sliding block projection
Portion 201a development length is less than the A/F of fluid channel 103；After cover plate 2 is placed on substrate 1, sliding block raised front portion 201a stops
Test sample liquid in liquid storage chamber 3 enters PCR reaction tanks 102；Fig. 3 is referred to, when slide cover 2, the sliding block is raised
201 move to the direction of liquid storage chamber 3, so that sliding block raised front portion 201a extruding liquid storage chamber 3；Passed through in sliding block projection 201
When crossing 103 opening of fluid channel, forward and backward gap is formed between the sliding block raised front portion 201a and the opening of fluid channel 103, with
Make to treat that sample measuring liquid body is let out from what is be made up of anterior diastema, fluid channel 103, post gap, discharging slot 202 in the liquid storage chamber 3
Release in press-in PCR reaction tanks 102.
From Fig. 2（a）In it can be seen that formed liquid storage chamber 3, direction such as Fig. 2 of slide cover 2（a）Middle direction arrow
Shown in head F；Entirely treat that the process of releasing of sample measuring liquid body refers to Fig. 3, liquid storage chamber 3 is by sliding block raised front portion 201a in Fig. 3
Extruding, treat that sample measuring liquid body borrows the opening of fluid channel 103 from both sides and entered in PCR reaction tanks 102, each PCR reaction tanks 102 are realized
Synchronous sample-adding operation, improves sample-adding efficiency, has saved loading time, the primer and probe material that it also avoid in reaction tank is few
Amount is mixed into other reaction tanks, causes more primed probes to mix, so that producing multi-products in one reaction pool, makes result can not
Interpretation.
After liquid storage chamber 3, which extrudes, to disappear, sliding block rear raised portion 201b covering PCR reaction tanks 102, so that PCR reacts
Pond 102 forms sealing space；Therefore, this array digital pcr chip is after completing each PCR reaction tanks 102 and being loaded simultaneously, you can
Realization is sealed each PCR reaction tanks 102, forms sealing space, therefore without excluding sample to be tested in addition mineral oil；And
And as a preferred embodiment, sample measuring liquid body dosage is treated required for each PCR reaction tanks 102 can be preset, with
Avoid treating that sample measuring liquid body remains as far as possible, Optimum Experiment operating procedure.
In Fig. 1 it can be seen that fluid channel 103 penetrates each liquid storage chamber 3, the A/F of fluid channel 103 can basis
Need to be configured, it is 50 to 70 microns to set scope, and sliding block raised front portion 201a development lengths are open width less than fluid channel 103
Degree, sets the glide direction F of sliding block projection 201 as forward slip, then sliding block raised front portion 201a forwards after mobile end, with
Make sliding block rear raised portion 201b covering PCR reaction tanks 102, PCR reaction tanks 102 can be square or rectangle, long
Degree can be at 140 to 180 microns, and above-mentioned size only enumerates, as long as being that by the other sizes of technical scheme
Also in the protection domain of the application.
Embodiment 2
On the basis of embodiment 1, the present embodiment 2 provides the digital pcr amplification instrument for array digital pcr chip,
And including automatic sample feeding device, realize that automatic sample operates to array digital pcr chip to realize.
As described in Example 1, here is omitted for the concrete structure of the array digital pcr chip.
The automatic sample feeding device includes：The sample-adding mouth of sample-adding operation is realized for inserting well 200a；And insertion
Negative pressure hole 200b carries out the negative pressure mouth of negative-pressure operation to liquid storage chamber 3；In sample-adding, treat that sample measuring liquid body is added to from sample-adding mouth
In array digital pcr chip, and extracted by negative pressure mouth, so that test sample liquid is full of in each liquid storage chamber 3.
By being loaded mouth and negative pressure mouth compounding practice, to realize quick sample-adding, sample-adding efficiency is improved.
Fig. 5 is pressing device 4 and the working state schematic representation of pushing meanss 5 in digital pcr amplification instrument.
Referring to Fig. 5, the digital pcr amplification instrument also includes：Pressing device 4 and pushing meanss 5；It is described before sample-adding
Pressing device 4 is suitable to compress substrate 1 with cover plate 2 to be bonded；And after sample-adding, the pushing mechanism is suitable to promote cover plate
2 slide, and to form runner of releasing at sliding block projection 201, make to treat that sample measuring liquid body passes through stream of accordingly releasing in each liquid storage chamber 3
Road flows into PCR reaction tanks 102.
The pressing device 4 includes：Elevating mechanism, and two press strips consistent with the glide direction of cover plate 2, and two press strips are fitted
In the upper surface both sides for pushing down cover plate 2 respectively；And it is provided with cushion 401 positioned at the lower surface of press strip.Wherein, the lift
Structure is suitable to realize that the effect of the cushion 401 is to make substrate 1 more be bonded with cover plate 2 using screw body or cylinder, with
To treating that sample measuring liquid body cannot be introduced into the gap of substrate 1 and cover plate 2.
The pushing meanss 5 are located at the rear side of the glide direction of cover plate 2, to promote cover plate 2 keeping impaction state with substrate 1
When slide.
The pushing meanss 5 can use screw body or cylinder to realize.
Specifically, being provided with processor module in the digital pcr amplification instrument, the processor module is such as, but not limited to adopted
With arm processor, or MSP430 single-chip microcomputers, and then automatic sample feeding device and pressing device 4 and pushing meanss 5 are controlled to work,
Common knowledge is partly belonged to as interlock circuit, is repeated no more here.
Using it is above-mentioned according to desirable embodiment of the present utility model as enlightenment, pass through above-mentioned description, related work people
Member can carry out various changes and amendments in the range of without departing from this item utility model technological thought completely.This item is real
The content being not limited to new technical scope on specification, it is necessary to which its technology is determined according to right
Property scope.
Claims (5)
- A kind of 1. digital pcr amplification instrument, it is characterised in thatThe digital pcr amplification instrument is adapted in use to array digital pcr chip；The digital pcr amplification instrument includes：Automatic sample feeding device, realize that automatic sample operates to array digital pcr chip.
- 2. digital pcr amplification instrument according to claim 1, it is characterised in thatThe array digital pcr chip includes substrate and cover plate, whereinThe upper surface of the substrate is placed with some chutes；The bottom surface medial recess of each chute forms a PCR reaction tanks；The underside view of part of the cover plate has some sliding block projections for being suitable to respectively engage with each chute；After cover plate is placed on substrate, chute corresponding to the raised engaging of sliding block, now, a liquid is vacated between sliding block projection and chute Body storage chamber；Fluid channel is offered on the chute wall of liquid storage chamber both sides, and is stored each liquid by the fluid channel Chamber is connected；The substrate is additionally provided with total runner, and liquid storage chamber is entered after sample measuring liquid body first passes through total runner, then by each fluid channel Sequentially enter respective liquid storage chamber；Well is offered on the cover plate, the perforate mouth of the well is directed at the head end of total runner；Negative pressure hole is further opened with the cover plate, the perforate mouth of the negative pressure hole is directed at the end of total runner；The automatic sample feeding device includes：The sample-adding mouth of sample-adding operation is realized for inserting well；And insertion negative pressure hole pair Liquid storage chamber carries out the negative pressure mouth of negative-pressure operation；In sample-adding, treat that sample measuring liquid body is added in array digital pcr chip from sample-adding mouth, and taken out by negative pressure mouth Take, so that test sample liquid is full of in each liquid storage chamber.
- 3. digital pcr amplification instrument according to claim 2, it is characterised in thatThe digital pcr amplification instrument also includes：Pressing device and pushing meanss；Before sample-adding, the pressing device, which is suitable to compress substrate with cover plate, to be bonded；AndAfter sample-adding, the pushing mechanism is suitable to promote cover plate to slide, and to form runner of releasing in sliding block high spot, makes each Treat that sample measuring liquid body flows into PCR reaction tanks by runner of accordingly releasing in liquid storage chamber.
- 4. digital pcr amplification instrument according to claim 3, it is characterised in thatThe pressing device includes：Elevating mechanism, and two press strips consistent with cover plate glide direction, and two press strips are suitable to difference Push down the upper surface both sides of cover plate；AndCushion is provided with positioned at the lower surface of press strip.
- 5. digital pcr amplification instrument according to claim 4, it is characterised in thatThe pushing meanss are located at the rear side of cover plate glide direction, to promote cover plate to be slided when keeping impaction state with substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720973020.6U CN207143217U (en) | 2017-08-03 | 2017-08-03 | Digital pcr amplification instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720973020.6U CN207143217U (en) | 2017-08-03 | 2017-08-03 | Digital pcr amplification instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207143217U true CN207143217U (en) | 2018-03-27 |
Family
ID=61674129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720973020.6U Expired - Fee Related CN207143217U (en) | 2017-08-03 | 2017-08-03 | Digital pcr amplification instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207143217U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365685A (en) * | 2017-08-03 | 2017-11-21 | 甘肃出入境检验检疫局检验检疫综合技术中心 | Digital pcr amplification instrument and its method of work |
| CN113063779A (en) * | 2021-03-15 | 2021-07-02 | 埃妥生物科技(杭州)有限公司 | Sampler and mixing device of sample and reagent |
-
2017
- 2017-08-03 CN CN201720973020.6U patent/CN207143217U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365685A (en) * | 2017-08-03 | 2017-11-21 | 甘肃出入境检验检疫局检验检疫综合技术中心 | Digital pcr amplification instrument and its method of work |
| CN113063779A (en) * | 2021-03-15 | 2021-07-02 | 埃妥生物科技(杭州)有限公司 | Sampler and mixing device of sample and reagent |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180327 Termination date: 20200803 |