CN207143217U - Digital pcr amplification instrument - Google Patents

Digital pcr amplification instrument Download PDF

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Publication number
CN207143217U
CN207143217U CN201720973020.6U CN201720973020U CN207143217U CN 207143217 U CN207143217 U CN 207143217U CN 201720973020 U CN201720973020 U CN 201720973020U CN 207143217 U CN207143217 U CN 207143217U
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CN
China
Prior art keywords
digital pcr
cover plate
sample
pcr amplification
amplification instrument
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720973020.6U
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Chinese (zh)
Inventor
李儒�
张宇霞
高黎红
李经纬
沙俊
刘鹏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitaschip Co Ltd
Inspection & Quarantine Technology Center Of Gansu Entry-Exit Inspection & Quarantine Bureau
Original Assignee
Mitaschip Co Ltd
Inspection & Quarantine Technology Center Of Gansu Entry-Exit Inspection & Quarantine Bureau
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitaschip Co Ltd, Inspection & Quarantine Technology Center Of Gansu Entry-Exit Inspection & Quarantine Bureau filed Critical Mitaschip Co Ltd
Priority to CN201720973020.6U priority Critical patent/CN207143217U/en
Application granted granted Critical
Publication of CN207143217U publication Critical patent/CN207143217U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

Biochemistry experiment instrument is the utility model is related to, specially a kind of digital pcr amplification instrument, the digital pcr amplification instrument is adapted in use to array digital pcr chip;The digital pcr amplification instrument includes:Automatic sample feeding device, realize that automatic sample operates to array digital pcr chip.The digital pcr amplification instrument of utility model is realized using array digital pcr chip to be treated sample measuring liquid body while is loaded onto in each PCR reaction tanks, avoid producing nonspecific reaction, specifically pass through substrate and cover plate compression fit, cover plate is slided under the cooperation of pressing device and pushing meanss, and then make the liquid in each liquid storage chamber while enter in corresponding PCR reaction tanks, mixed with primer and probe in reaction tank, avoid producing nonspecific reaction, improve the accuracy of subsequent detection data.

Description

Digital pcr amplification instrument
Technical field
It the utility model is related to biochemistry experiment instrument, and in particular to a kind of array digital pcr chip.
Background technology
PCR(PCR)It has been widely used in medical science, science of heredity, microbiology or even whole life science In.Real-time quantitative PCR array technique is using Real-time quantitative PCR as core, and combines array technique, realizes single experiment A kind of new amplification technique of detectable series bulk gene, the technology being capable of reliable, scale detection signals exactly The related gene expression profile of the important biomolecule processes such as transduction, disease related pathways, in microorganism, hereditary disease, tumour, drug gene There is important application in the subjects such as group.
In the prior art, digital polymerase chain reaction is performed using base material design chips(Digital PCR).Pass through two The overlapping loading of the individual glass slide with pore passage structure, merging two sides duct turns into a complete runner, and in the substrate Provided with the how individual reaction tanks of N, runner will treat that sample measuring liquid body is sequentially added in each reaction tank, and this time difference will shift to an earlier date partial reaction pond Sample measuring liquid body is treated in acquisition, is mixed with primer and probe in reaction tank, produces nonspecific reaction, rather than specific PCR production Thing will produce interference to testing result;And the primer and probe material in the reaction tank of runner front portion is easy due to liquid flowing impulse force It is brought on a small quantity in next reaction tank, more primed probe mixing, produces multi-products in one reaction pool, cause result can not Interpretation;And there has been no a digital pcr amplification instrument that can promote to treat sample measuring liquid body while be added to each PCR reaction tanks.
Utility model content
The purpose of this utility model is to provide a kind of digital pcr amplification instrument, and it is anti-to treat that sample measuring liquid body is loaded onto each PCR with guarantee Pond while property is answered, avoids producing nonspecific reaction.
In order to solve the above-mentioned technical problem, the utility model provides a kind of digital pcr amplification instrument, and the digital pcr expands Increase instrument and be adapted in use to array digital pcr chip;The digital pcr amplification instrument includes:Automatic sample feeding device, to array numeral Pcr chip realizes that automatic sample operates.
Further, the array digital pcr chip includes substrate and cover plate, wherein
The upper surface of the substrate is placed with some chutes;The bottom surface medial recess of each chute forms a PCR reaction tanks;
The underside view of part of the cover plate has some sliding block projections for being suitable to respectively engage with each chute;
After cover plate is placed on substrate, chute corresponding to the raised engaging of sliding block, now, vacated between sliding block projection and chute One liquid storage chamber;
Offer fluid channel on the chute wall of liquid storage chamber both sides, and by the fluid channel by each liquid Storage chamber is connected;
The substrate is additionally provided with total runner, and liquid storage chamber is entered after sample measuring liquid body first passes through total runner, then by each micro- Runner sequentially enters respective liquid storage chamber.
Well is offered on the cover plate, the perforate mouth of the well is directed at the head end of total runner;
Negative pressure hole is further opened with the cover plate, the perforate mouth of the negative pressure hole is directed at the end of total runner;
The automatic sample feeding device includes:The sample-adding mouth of sample-adding operation is realized for inserting well;And insertion negative pressure Hole carries out the negative pressure mouth of negative-pressure operation to liquid storage chamber;
In sample-adding, treat that sample measuring liquid body is added in array digital pcr chip from sample-adding mouth, and enter by negative pressure mouth Row extracts, so that test sample liquid is full of in each liquid storage chamber.
Further, the digital pcr amplification instrument also includes:Pressing device and pushing meanss;
Before sample-adding, the pressing device, which is suitable to compress substrate with cover plate, to be bonded;And
After sample-adding, the pushing mechanism is suitable to promote cover plate to slide, to form runner of releasing in sliding block high spot, So as to treat that sample measuring liquid body flows into PCR reaction tanks by runner of accordingly releasing in each liquid storage chamber.
Further, the pressing device includes:Elevating mechanism, and two press strips consistent with cover plate glide direction, and two Press strip is suitable to the upper surface both sides for pushing down cover plate respectively;And
Cushion is provided with positioned at the lower surface of press strip.
Further, the pushing meanss are located at the rear side of cover plate glide direction, to promote cover plate keeping compressing with substrate Slided during state.
The beneficial effects of the utility model are that digital pcr amplification instrument of the present utility model uses array digital pcr chip Realization is treated sample measuring liquid body while is loaded onto in each PCR reaction tanks, avoids producing nonspecific reaction, specifically passes through substrate and lid Plate compression fit, cover plate is slided under the cooperation of pressing device and pushing meanss, and then make the liquid in each liquid storage chamber Enter simultaneously in corresponding PCR reaction tanks, mixed with primer and probe in reaction tank, avoid producing nonspecific reaction, carry The accuracy of high subsequent detection data.
Brief description of the drawings
The utility model is further illustrated with reference to the accompanying drawings and examples.
Fig. 1(a)It is the structural representation of array digital pcr chip of the present utility model
Fig. 1(b)It is the structural representation and partial enlarged drawing of substrate of the present utility model;
Fig. 2(a)It is the structural representation of chute corresponding to the raised engaging of sliding block of the present utility model;
Fig. 2(b)It is Fig. 2(a)The profile of middle A-A positions;
The sliding block raised schematic diagram that runner of releasing is formed with chute when Fig. 3 is slide cover;
Fig. 4(a)It is the sliding block projection and chute fit structure schematic diagram after the extruding of liquid storage chamber disappears;
Fig. 4(b)It is Fig. 4(a)The profile of middle B-B positions;
Fig. 5 is pressing device and the working state schematic representation of pushing meanss in digital pcr amplification instrument.
In figure:
Substrate 1, total runner 100, the head end 100a of total runner, chute 101, PCR reaction tanks 102, fluid channel 103;
Cover plate 2, well 200a, negative pressure hole 200b, sliding block projection 201, discharging slot 202, sliding block raised front portion 201a, cunning Block rear raised portion 201b;
Liquid storage chamber 3;
Pressing device 4, cushion 401,
Pushing meanss 5.
Embodiment
The utility model is described in further detail presently in connection with accompanying drawing.These accompanying drawings are simplified schematic diagram, Only illustrate basic structure of the present utility model in a schematic way, therefore it only shows the composition relevant with the utility model.
In the prior art, digital polymerase chain reaction is performed using base material design chips(Digital PCR).Pass through two The overlapping loading of the individual glass slide with pore passage structure, merging two sides duct turns into a complete runner, and in the substrate Provided with the how individual reaction tanks of N, runner will treat that sample measuring liquid body is sequentially added in each reaction tank, and this time difference will shift to an earlier date partial reaction pond Sample measuring liquid body is treated in acquisition, is mixed with primer and probe in reaction tank, produces nonspecific reaction, and nonspecific products will Interference is produced to testing result;And the primer and probe material in the reaction tank of runner front portion is easily a small amount of due to liquid flowing impulse force It is brought into next reaction tank, more primed probe mixing, produces multi-products in one reaction pool, cause result not sentence Read;And there is not a digital pcr amplification instrument that can promote to treat sample measuring liquid body while be added to each PCR reaction tanks yet.
In order to solve the above-mentioned technical problem, this array digital pcr chip, digital pcr are expanded by following examples Instrument is described in detail.
Fig. 1(a)It is the structural representation of array digital pcr chip of the present utility model
Fig. 1(b)It is the structural representation and partial enlarged drawing of substrate 1 of the present utility model;
Fig. 2(a)It is the structural representation of chute 101 corresponding to the engaging of sliding block projection 201 of the present utility model;
Fig. 2(b)It is Fig. 2(a)The profile of middle A-A positions;
Refer to Fig. 1(a)To Fig. 2(b)Shown, the present embodiment 1 provides a kind of array digital pcr chip, including:
Substrate 1 and cover plate 2, wherein
The upper surface of the substrate 1 is placed with some chutes 101;It is anti-that the bottom surface medial recess of each chute 101 forms a PCR Answer pond 102;The underside view of part of the cover plate 2 has some sliding block projections 201 suitable for engaging respectively with each chute 101;Work as lid After plate 2 is placed on substrate 1, chute 101 corresponding to the engaging of sliding block projection 201 is now, empty between sliding block projection 201 and chute 101 Go out a liquid storage chamber 3;It is raised in sliding block by slide cover 2 when after each liquid storage chamber 3 is each filled with after sample measuring liquid body Runner of releasing is formed at 201, so as to treat that sample measuring liquid body flows into PCR reaction tanks 102 by runner of accordingly releasing.
This array digital pcr chip is coordinated by substrate 1 and cover plate 2, in the promotion lower cover 2 of digital pcr amplification instrument Slide, make the liquid in each liquid storage chamber 3 while enter in corresponding PCR reaction tanks 102, enter with primer and probe in reaction tank Row mixing, avoid producing nonspecific reaction, improve the accuracy of subsequent detection data.
Refer to Fig. 1(b), fluid channel 103 is offered in the side wall of chute 101 of the both sides of liquid storage chamber 3, and lead to Cross the fluid channel 103 each liquid storage chamber 3 is connected;The substrate 1 is additionally provided with total runner 100, treats that sample measuring liquid body first passes through Enter liquid storage chamber 3 after total runner 100, then respective liquid storage chamber 3 is sequentially entered by each fluid channel 103.
The perforate mouth that well 200a, the well 200a are offered on the cover plate 2 is directed at the head end of total runner 100a(It is indicated in Figure 5);
The perforate mouth that negative pressure hole 200b, the negative pressure hole 200b are further opened with the cover plate 2 is directed at the end of total runner 100 End.
Wherein, well 200a such as, but not limited to uses horn mouth, and big mouth is set outwardly.
Sliding block projection 201 forms the schematic diagram for runner of releasing with chute 101 when Fig. 3 is slide cover 2;
Fig. 4(a)It is the sliding block projection 201 and the fit structure schematic diagram of chute 101 after the extruding of liquid storage chamber 3 disappears;
Fig. 4(b)It is Fig. 4(a)The profile of middle B-B positions.
Refer to Fig. 2(a)To Fig. 4(b), the chute 101 is cuboid, and the fluid channel 103 is located at chute 101 In long side wall, 201 cuboid to be fastened with the chute 101 of the sliding block projection;The bottom surface of the sliding block projection 201 It is in contact with the bottom surface of chute 101;A discharging slot 202 is opened up on the bottom surface of the sliding block projection 201, sliding block is raised 201 points are front and rear;Raised 201 engagement directions of the direction of the discharging slot 202 and sliding block are orthogonal, and before making sliding block projection Portion 201a development length is less than the A/F of fluid channel 103;After cover plate 2 is placed on substrate 1, sliding block raised front portion 201a stops Test sample liquid in liquid storage chamber 3 enters PCR reaction tanks 102;Fig. 3 is referred to, when slide cover 2, the sliding block is raised 201 move to the direction of liquid storage chamber 3, so that sliding block raised front portion 201a extruding liquid storage chamber 3;Passed through in sliding block projection 201 When crossing 103 opening of fluid channel, forward and backward gap is formed between the sliding block raised front portion 201a and the opening of fluid channel 103, with Make to treat that sample measuring liquid body is let out from what is be made up of anterior diastema, fluid channel 103, post gap, discharging slot 202 in the liquid storage chamber 3 Release in press-in PCR reaction tanks 102.
From Fig. 2(a)In it can be seen that formed liquid storage chamber 3, direction such as Fig. 2 of slide cover 2(a)Middle direction arrow Shown in head F;Entirely treat that the process of releasing of sample measuring liquid body refers to Fig. 3, liquid storage chamber 3 is by sliding block raised front portion 201a in Fig. 3 Extruding, treat that sample measuring liquid body borrows the opening of fluid channel 103 from both sides and entered in PCR reaction tanks 102, each PCR reaction tanks 102 are realized Synchronous sample-adding operation, improves sample-adding efficiency, has saved loading time, the primer and probe material that it also avoid in reaction tank is few Amount is mixed into other reaction tanks, causes more primed probes to mix, so that producing multi-products in one reaction pool, makes result can not Interpretation.
After liquid storage chamber 3, which extrudes, to disappear, sliding block rear raised portion 201b covering PCR reaction tanks 102, so that PCR reacts Pond 102 forms sealing space;Therefore, this array digital pcr chip is after completing each PCR reaction tanks 102 and being loaded simultaneously, you can Realization is sealed each PCR reaction tanks 102, forms sealing space, therefore without excluding sample to be tested in addition mineral oil;And And as a preferred embodiment, sample measuring liquid body dosage is treated required for each PCR reaction tanks 102 can be preset, with Avoid treating that sample measuring liquid body remains as far as possible, Optimum Experiment operating procedure.
In Fig. 1 it can be seen that fluid channel 103 penetrates each liquid storage chamber 3, the A/F of fluid channel 103 can basis Need to be configured, it is 50 to 70 microns to set scope, and sliding block raised front portion 201a development lengths are open width less than fluid channel 103 Degree, sets the glide direction F of sliding block projection 201 as forward slip, then sliding block raised front portion 201a forwards after mobile end, with Make sliding block rear raised portion 201b covering PCR reaction tanks 102, PCR reaction tanks 102 can be square or rectangle, long Degree can be at 140 to 180 microns, and above-mentioned size only enumerates, as long as being that by the other sizes of technical scheme Also in the protection domain of the application.
Embodiment 2
On the basis of embodiment 1, the present embodiment 2 provides the digital pcr amplification instrument for array digital pcr chip, And including automatic sample feeding device, realize that automatic sample operates to array digital pcr chip to realize.
As described in Example 1, here is omitted for the concrete structure of the array digital pcr chip.
The automatic sample feeding device includes:The sample-adding mouth of sample-adding operation is realized for inserting well 200a;And insertion Negative pressure hole 200b carries out the negative pressure mouth of negative-pressure operation to liquid storage chamber 3;In sample-adding, treat that sample measuring liquid body is added to from sample-adding mouth In array digital pcr chip, and extracted by negative pressure mouth, so that test sample liquid is full of in each liquid storage chamber 3.
By being loaded mouth and negative pressure mouth compounding practice, to realize quick sample-adding, sample-adding efficiency is improved.
Fig. 5 is pressing device 4 and the working state schematic representation of pushing meanss 5 in digital pcr amplification instrument.
Referring to Fig. 5, the digital pcr amplification instrument also includes:Pressing device 4 and pushing meanss 5;It is described before sample-adding Pressing device 4 is suitable to compress substrate 1 with cover plate 2 to be bonded;And after sample-adding, the pushing mechanism is suitable to promote cover plate 2 slide, and to form runner of releasing at sliding block projection 201, make to treat that sample measuring liquid body passes through stream of accordingly releasing in each liquid storage chamber 3 Road flows into PCR reaction tanks 102.
The pressing device 4 includes:Elevating mechanism, and two press strips consistent with the glide direction of cover plate 2, and two press strips are fitted In the upper surface both sides for pushing down cover plate 2 respectively;And it is provided with cushion 401 positioned at the lower surface of press strip.Wherein, the lift Structure is suitable to realize that the effect of the cushion 401 is to make substrate 1 more be bonded with cover plate 2 using screw body or cylinder, with To treating that sample measuring liquid body cannot be introduced into the gap of substrate 1 and cover plate 2.
The pushing meanss 5 are located at the rear side of the glide direction of cover plate 2, to promote cover plate 2 keeping impaction state with substrate 1 When slide.
The pushing meanss 5 can use screw body or cylinder to realize.
Specifically, being provided with processor module in the digital pcr amplification instrument, the processor module is such as, but not limited to adopted With arm processor, or MSP430 single-chip microcomputers, and then automatic sample feeding device and pressing device 4 and pushing meanss 5 are controlled to work, Common knowledge is partly belonged to as interlock circuit, is repeated no more here.
Using it is above-mentioned according to desirable embodiment of the present utility model as enlightenment, pass through above-mentioned description, related work people Member can carry out various changes and amendments in the range of without departing from this item utility model technological thought completely.This item is real The content being not limited to new technical scope on specification, it is necessary to which its technology is determined according to right Property scope.

Claims (5)

  1. A kind of 1. digital pcr amplification instrument, it is characterised in that
    The digital pcr amplification instrument is adapted in use to array digital pcr chip;The digital pcr amplification instrument includes:
    Automatic sample feeding device, realize that automatic sample operates to array digital pcr chip.
  2. 2. digital pcr amplification instrument according to claim 1, it is characterised in that
    The array digital pcr chip includes substrate and cover plate, wherein
    The upper surface of the substrate is placed with some chutes;The bottom surface medial recess of each chute forms a PCR reaction tanks;
    The underside view of part of the cover plate has some sliding block projections for being suitable to respectively engage with each chute;
    After cover plate is placed on substrate, chute corresponding to the raised engaging of sliding block, now, a liquid is vacated between sliding block projection and chute Body storage chamber;
    Fluid channel is offered on the chute wall of liquid storage chamber both sides, and is stored each liquid by the fluid channel Chamber is connected;
    The substrate is additionally provided with total runner, and liquid storage chamber is entered after sample measuring liquid body first passes through total runner, then by each fluid channel Sequentially enter respective liquid storage chamber;
    Well is offered on the cover plate, the perforate mouth of the well is directed at the head end of total runner;
    Negative pressure hole is further opened with the cover plate, the perforate mouth of the negative pressure hole is directed at the end of total runner;
    The automatic sample feeding device includes:The sample-adding mouth of sample-adding operation is realized for inserting well;And insertion negative pressure hole pair Liquid storage chamber carries out the negative pressure mouth of negative-pressure operation;
    In sample-adding, treat that sample measuring liquid body is added in array digital pcr chip from sample-adding mouth, and taken out by negative pressure mouth Take, so that test sample liquid is full of in each liquid storage chamber.
  3. 3. digital pcr amplification instrument according to claim 2, it is characterised in that
    The digital pcr amplification instrument also includes:Pressing device and pushing meanss;
    Before sample-adding, the pressing device, which is suitable to compress substrate with cover plate, to be bonded;And
    After sample-adding, the pushing mechanism is suitable to promote cover plate to slide, and to form runner of releasing in sliding block high spot, makes each Treat that sample measuring liquid body flows into PCR reaction tanks by runner of accordingly releasing in liquid storage chamber.
  4. 4. digital pcr amplification instrument according to claim 3, it is characterised in that
    The pressing device includes:Elevating mechanism, and two press strips consistent with cover plate glide direction, and two press strips are suitable to difference Push down the upper surface both sides of cover plate;And
    Cushion is provided with positioned at the lower surface of press strip.
  5. 5. digital pcr amplification instrument according to claim 4, it is characterised in that
    The pushing meanss are located at the rear side of cover plate glide direction, to promote cover plate to be slided when keeping impaction state with substrate.
CN201720973020.6U 2017-08-03 2017-08-03 Digital pcr amplification instrument Expired - Fee Related CN207143217U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720973020.6U CN207143217U (en) 2017-08-03 2017-08-03 Digital pcr amplification instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720973020.6U CN207143217U (en) 2017-08-03 2017-08-03 Digital pcr amplification instrument

Publications (1)

Publication Number Publication Date
CN207143217U true CN207143217U (en) 2018-03-27

Family

ID=61674129

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720973020.6U Expired - Fee Related CN207143217U (en) 2017-08-03 2017-08-03 Digital pcr amplification instrument

Country Status (1)

Country Link
CN (1) CN207143217U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107365685A (en) * 2017-08-03 2017-11-21 甘肃出入境检验检疫局检验检疫综合技术中心 Digital pcr amplification instrument and its method of work
CN113063779A (en) * 2021-03-15 2021-07-02 埃妥生物科技(杭州)有限公司 Sampler and mixing device of sample and reagent

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107365685A (en) * 2017-08-03 2017-11-21 甘肃出入境检验检疫局检验检疫综合技术中心 Digital pcr amplification instrument and its method of work
CN113063779A (en) * 2021-03-15 2021-07-02 埃妥生物科技(杭州)有限公司 Sampler and mixing device of sample and reagent

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Granted publication date: 20180327

Termination date: 20200803