CN113057063A - Cutting seedling method of akebia trifoliata - Google Patents
Cutting seedling method of akebia trifoliata Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention belongs to the technical field of akebia trifoliata koidz seedling culture, and provides a cutting seedling culture method of akebia trifoliata koidz. The method provided by the invention comprises the following steps: (1) selecting rattans with 1-2 complete stem segments and 1-2 buds as cuttings; (2) scratching treatment is carried out on stem sections within the range of 2-4 cm above the cutting ends of the cuttings, and only the epidermis is scratched without damaging the phloem; (3) soaking the cut slips subjected to the scratching treatment in a nutrient solution, and taking out the slips for pre-rooting treatment; (4) and (4) carrying out cuttage cultivation on the cuttings treated in the step (3) to obtain the akebia trifoliata cuttage seedlings. The survival rate and the seedling rate of the akebia trifoliata cuttings can be remarkably improved, the survival rate of the cuttings is 98-100%, and the survival cuttings can be 100% seedlings; the seedling raising period can be obviously shortened, and the seedling raising time of the akebia trifoliata is shortened from 57-70 days to 20-25 days.
Description
Technical Field
The invention relates to the technical field of akebia trifoliata koidz seedling culture, in particular to a cutting seedling culture method of akebia trifoliata koidz.
Background
Akebia trifoliata (scientific name: Akebia trifoliata (Thunb.) Koidz.) is a plant of Akebia deciduous Wood vine of Akebia. The stem bark is grayish brown, and palm-shaped compound leaves are grown alternately or clustered on short branches; straight petiole, paper or thin leather of leaf, oval to broad oval, with blunt or slightly concave tip, truncated or rounded base, wavy teeth or light cleft at edge, dark green above, light green below; the raceme is extracted from the clustered leaves on the short branches, and the total pedicel is fine. Long round, straight or slightly curved, extremely numerous seeds, flat ovoid, reddish brown or dark brown in the seed coat, slightly lustrous. Blooming in 4-5 months and bearing fruit in 7-8 months. Is distributed in Hebei, Shanxi, Shandong, Henan, southern Shaanxi, southeast of Gansu to the provinces of Yangtze river basin in China. Growing in sparse forest at the valley side of mountainous regions with the elevation of 250-2000 m or irrigation hills. The root, stem and fruit can be used as medicine, has effects of promoting diuresis, promoting lactation, relieving rigidity of muscles and activating collaterals, and is mainly used for treating rheumatic arthralgia; fruits can be eaten or brewed; the seed can be used for squeezing oil.
At present, seedling raising methods of akebia trifoliata mainly comprise seed seedling raising, layering seedling raising, pocket-separated seedling raising, cutting seedling raising and the like. The seed is simple and easy to grow, the seedling rate is high, but the breeding period is long, the fruit can be grown in 3 years generally, and the seed is less used in production; the branches of the akebia trifoliate are of a comprehensive rooting type (the bark roots are mainly rooted), and the rattan germination capacity is strong, so layering seedling culture is also adopted in production, the shoots can be rooted after layering seedling culture for one month, the shoots can blossom and bear fruits in the second field planting, compared with seed seedling culture, the seedling culture period is greatly shortened, but layering seedling culture is greatly influenced by mother plants and limited by land area, the operation is inconvenient, and the implementation is difficult; the split-pocket seedling culture is to separate a plurality of akebia trifoliata plants with one stump and a plurality of plants from roots, and the akebia trifoliata plants can bear fruits in the current year after being planted in the current year under the condition of not cutting off branches and tendrils, but similar to layering seedling culture, the method is influenced by mother plants and limited by land, is inconvenient to operate and cannot realize large-scale production; the cuttage seedling raising method is characterized in that strong and disease and pest free 1-2-year-old branch vines are selected for cuttage, the method is not limited by mother plants and land, the operation is convenient, large-scale planting can be realized, cuttage can be carried out all the year round, the method is not limited by time, the method is the most main seedling raising method of akebia trifoliata, but the survival rate of the cuttage seedling raising is generally 50-90%, the seedling rate is lower by 30-60%, and the method is the main bottleneck restricting the development of the cuttage seedling raising of akebia trifoliata at present.
Disclosure of Invention
The invention provides the following technical scheme in order to solve the problems of long seedling growing period, low survival rate and low seedling rate of akebia trifoliata.
A cutting seedling method of akebia trifoliata includes the following steps:
(1) selecting rattans with 1-2 complete stem segments and 1-2 buds as cuttings;
(2) scratching treatment is carried out on stem sections within the range of 2-4 cm above the cutting ends of the cuttings, and only the epidermis is scratched without damaging the phloem;
(3) soaking the cut slips subjected to the scratching treatment in a nutrient solution, and taking out the slips for pre-rooting treatment;
(4) and (4) carrying out cuttage cultivation on the cuttings treated in the step (3) to obtain the akebia trifoliata cuttage seedlings.
Preferably, the scoring treatment is an "X" or "+" shaped wound made on the epidermis.
Preferably, each scratch wound of the X-shaped wound or the plus-shaped wound has a length of 2-4 mm.
Preferably, the density of the X-shaped or the plus-shaped wound is 1-4/cm2。
Preferably, the nutrient solution comprises urea, disodium hydrogen phosphate, calcium sulfate and water, and the total concentration is 0.024-0.05 g/L.
Preferably, the mass ratio of urea to disodium hydrogen phosphate to calcium sulfate in the nutrient solution is 4-5: 2-3: 1-2.
Preferably, the nutrient solution is soaked for 2-5 hours.
Preferably, the pre-rooting treatment is to treat the cuttings in a dark environment with the temperature of 30-35 ℃ and the humidity of 80-90% for 18-24 hours.
Preferably, the cuttings obtained in the step (4) can be transplanted after being cultivated for 20-25 days.
The method for raising the seedlings of akebia trifoliata dunn provided by the invention can obviously improve the survival rate and the seedling rate of akebia trifoliata dunn cuttings, the survival rate of the cuttings is 98-100%, and the survival cuttings can be 100% seedlings; the seedling raising period can be obviously shortened, and the seedling raising time of the akebia trifoliata is shortened to 20-25 days from 57-70 days.
Detailed Description
The cutting seedling raising is the most widely and mainly applied one of the numerous akebia trifoliata seedling raising methods, and compared with other seedling raising methods, the cutting seedling raising has low requirements on mother plants and can be propagated by only adopting proper rattans; because the stock plants are separated for propagation, the applicable land range is wide, and the cutting operation is convenient; and the rooting type is a comprehensive rooting type which takes the rooting type of the bark as the main part and takes the callus as the auxiliary part, so the method is more suitable for the breeding mode of cutting seedling. However, after the akebia trifoliate is rooted, the roots of the roots are few, the water and nutrient absorption is slow or even untimely, so that the roots of the cuttage branches grow slowly and the branches sprout late, and the cuttage branches are seriously withered or rotten, so that the survival rate of the akebia trifoliate cuttings is low, and the seedling rate is lower. Therefore, the method solves the rooting problem of the akebia trifoliata cutting branches, and is the key for improving the survival rate and the seedling rate of the akebia trifoliata cutting seedlings and shortening the cultivation period.
Therefore, the invention provides a cutting seedling method of akebia trifoliata, which comprises the following steps:
(1) selecting rattans with 1-2 complete stem segments and 1-2 buds as cuttings;
(2) scratching treatment is carried out on stem sections within the range of 2-4 cm above the cutting ends of the cuttings, and only the epidermis is scratched without damaging the phloem;
(3) soaking the cut slips subjected to the scratching treatment in a nutrient solution, and taking out the slips for pre-rooting treatment;
(4) and (4) carrying out cuttage cultivation on the cuttings treated in the step (3) to obtain the akebia trifoliata cuttage seedlings.
The method comprises the steps of selecting 1-2 years old akebia trifoliata stems, cutting branches with 1-2 complete stem sections to serve as cuttings, and preferably selecting branches with 2 complete stem sections to serve as cuttings; preferably, the cuttings have 1-2 buds, and further preferably have 2 buds; and (5) standby.
In the present invention, the length of the cutting is preferably 15 to 20cm, more preferably 15cm, 16cm, 17cm, 18cm, 19cm, 20cm, and still more preferably 18 cm.
In the present invention, the diameter of the cutting is preferably 0.5 to 1.0cm, more preferably 0.5cm, 0.6cm, 0.7cm, 0.8cm, 0.9cm, 1.0cm, and still more preferably 0.7 cm.
According to the method, scratching treatment is carried out on stem sections within a range of 2-4 cm above the cutting end of the cutting slips, preferably scratching treatment is carried out within a range of 2cm, 3cm or 4cm above the cutting end, and further preferably scratching treatment is carried out within a range of 3cm above the cutting end.
In the invention, the scratching treatment only scratches the surface of the cutting without damaging the phloem; the scarification of the epidermis is beneficial to the protrusion of the root primordium under the epidermis and the early contact with the external water and nutrients, thereby accelerating the growth and development of the root, improving the survival rate and the seedling rate of the cuttings and shortening the seedling period; in addition, the scratching treatment only scratches the epidermis without damaging the phloem and does not damage the root primitive tissues under the epidermis; the seedling raising method of the invention mainly takes the rooting of the bark as the main part and plays the rooting role of the root primordium to the maximum extent.
In the present invention, the scratching treatment preferably makes an "X" or "+" shaped wound on the epidermis, and more preferably an "X" shaped wound.
In the invention, the length of each scratch wound of the X-shaped or the plus-shaped wound is preferably 2-4 mm, more preferably 2mm, 3mm, 4mm, and still more preferably 3 mm.
In the invention, the density of the X-shaped or the plus-shaped wound is preferably 1-4/cm2More preferably 1 piece/cm22 pieces/cm23 pieces/cm24 pieces/cm2Still more preferably 4/cm2。
The cutting after the scratching treatment is soaked in nutrient solution and taken out for pre-rooting treatment.
In the invention, the nutrient solution comprises urea, disodium hydrogen phosphate, calcium sulfate and water, and the total concentration is preferably 0.024-0.05 g/L, more preferably 0.036-0.04 g/L, and even more preferably 0.04 g/L.
In the present invention, the mass ratio of urea, disodium hydrogen phosphate and calcium sulfate in the nutrient solution is preferably 4-5: 2-3: 1-2, more preferably 4:2:2, 4:3:1, 5:2:2, 4:3:2, 5:3:2, and more preferably 5:2: 1.
In the invention, the soaking time of the nutrient solution is preferably 2-5 h, more preferably 2h, 3h, 4h, 5h, and even more preferably 4 h.
The nutrient solution mainly provides nutrients and water for the cuttings, and avoids the problems that the nutrients and water of the cuttings are excessively lost in the subsequent pregermination and rooting processes, so that the leaf buds grow and develop slowly, and even after cuttage, the water and the nutrients of the cuttings are exhausted, and the cuttings are dry and dead.
In the invention, the cutting is taken out from the nutrient solution, and then is soaked in 100mg/L rooting powder for 30min and then is subjected to pre-rooting treatment.
In the invention, the pre-rooting treatment is to treat the cuttings in a dark environment with the temperature of 30-35 ℃ and the humidity of 80-90% for 18-24 hours.
In the invention, the temperature of the pre-rooting treatment is preferably 30-35 ℃, more preferably 30 ℃, 31 ℃, 32 ℃, 33 ℃, 34 ℃ and 35 ℃, and still more preferably 35 ℃; the humidity is preferably 80-90%, more preferably 80%, 82%, 85%, 87%, 89%, 90%, and even more preferably 89%; the time is preferably 18 to 24 hours, more preferably 18 hours, 20 hours, 22 hours and 24 hours, and still more preferably 22 hours.
The pre-rooting treatment of the invention mainly provides a high-temperature and high-humidity rooting environment for the cuttings, and aims to accelerate the protrusion speed of the root primordium so as to facilitate the cuttings to root as soon as possible after cutting. Meanwhile, the pre-rooting treatment is carried out in dark and humid environment, which is beneficial to softening the epidermis of the cutting and leading the expanded root primordial tissue to break through the epidermis more easily.
The cutting cultivation method carries out cutting cultivation on the cuttings after the pre-rooting treatment to obtain the akebia trifoliata cutting seedlings.
In the present invention, the cuttings are preferably cultivated for 20 to 25 days and then transplanted, more preferably cultivated for 20 days, 22 days, 23 days, 25 days and then transplanted, and even more preferably cultivated for 23 days and then transplanted. Compared with the technology which needs 57-70 days for transplanting in the prior art, the seedling raising time is shortened by more than one time, and the purpose of shortening the seedling raising period is achieved.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
In this example, 2-year-old akebia trifoliate stems are used as mother plants, 500 cuttings are cut in month 2, and different types of cuttings are cut according to table 1.
TABLE 1 types of slips
| Group of | Number of plants | Whole stem segment/ | Bud/an | Length of/cm | Diameter/cm |
| Group A | 200 | 2 | 2 | 18 | 0.5～0.7 |
| Group B | 100 | 2 | 1 | 20 | 0.7～0.9 |
| Group C | 100 | 1 | 2 | 16 | 0.7～1.0 |
| Group D | 100 | 1 | 1 | 15 | 1.0 |
And scratching the trimmed cuttings, wherein the scratching treatment depth only scratches the surface skin without damaging the phloem, and the scratching treatment mode is shown in table 2.
TABLE 2 scratch treatment mode
Note: the scratch range is 2-4 cm upwards from the cutting end of the cutting;
and (3) after scratching all the cuttings, completely soaking the cuttings in a nutrient solution with the total concentration of 0.04g/L, which is prepared according to the mass ratio of urea to disodium hydrogen phosphate to calcium sulfate of 5:2:1, for 4 hours. Taking out, soaking all cuttings in 100mg/L rooting powder for 30min, and then carrying out pre-rooting treatment. In this example, the pre-rooting treatment was carried out in a dark environment at 35 ℃ and 89% humidity for 22 hours. Observing the cuttings after the pre-rooting treatment, the cuttings are softened more than before the treatment, a plurality of roots and primordia are protruded at the scratched wound, even white radicles can be seen, and statistics shows that 367 cuttings with the white radicles are provided, account for 73.4 percent of the total cuttings, and are protruded from the scratched part. This indicates that the protrusion of the root primordium can be promoted by the scratching treatment and the pre-rooting treatment, and the growth and development of the root can be accelerated.
After counting, all cuttings are inserted into soil for cultivation, the number of dead seedlings and the survival rate are counted on the 10 th day of cultivation, the number of dead seedlings and the seedling rate are counted on the 20 th day, and the results are shown in table 3.
TABLE 3 statistics of cuttings survival rate and seedling rate
Note: survival percent is (number of cutting plants-number of dead seedlings at 10 th day)/number of cutting plants 100%; the seedling rate is (number of cutting plants-number of dead seedlings at 20 days)/number of cutting plants 100%.
As can be seen from Table 3, the survival rate and the seedling rate of the cutting seedling method are 99.2% on average, the survival rate and the seedling rate of the cuttings are greatly improved, and 100% of survival cuttings can be guaranteed to be seedlings. And the cutting of the invention can observe brown root system at 20 days of cutting cultivation, namely fixed root is formed, at this time, the cultivated seedling can be transplanted, and the seedling can bear fruit in the current year after transplantation.
Example 2
In the example, 100 cuttings are trimmed and scratched in the manner of group A1 in example 1 to serve as group E, and then soaked in a nutrient solution with a total concentration of 0.024g/L prepared from urea, disodium hydrogen phosphate and calcium sulfate in a mass ratio of 4:2:2 for 5 hours; taking out, and soaking in 100mg/L rooting powder for 30 min; then placing the roots in a dark environment with the temperature of 30 ℃ and the humidity of 90 percent for 24 hours for pre-rooting treatment. The number and proportion of cuttings with white radicles were observed and counted, 72 plants and 72% respectively, and all protruded from the scratch. All cuttings of this example were then planted in soil for cultivation at the same time as in example 1, and the number of dead seedlings and survival rate were counted on the 10 th day of cultivation, and the number of dead seedlings and seedling rate were counted on the 25 th day, and the results are shown in table 4.
TABLE 4E statistics of survival rate and seedling rate of the group cuttings
Note: survival percent is (number of cutting plants-number of dead seedlings at 10 th day)/number of cutting plants 100%; the seedling rate is (number of cutting plants-number of dead seedlings on 25 th day)/number of cutting plants 100%.
Example 3
In the embodiment, 100 cuttings are trimmed and scratched according to the treatment mode of group A1 in example 1 to serve as group F, and then the cuttings are soaked in a nutrient solution with the total concentration of 0.05g/L, which is prepared according to the mass ratio of urea to disodium hydrogen phosphate to calcium sulfate of 5:3:2, for 2 hours; taking out, and soaking in 100mg/L rooting powder for 30 min; then placing the roots in a dark environment with the temperature of 32 ℃ and the humidity of 80% for 18h for pre-rooting treatment. The number and proportion of cuttings with white radicles were observed and counted, 73 plants and 73% respectively, and all protruded from the scratch. All cuttings of this example were then planted in soil for cultivation at the same time as in example 1, and the number of dead seedlings and survival rate were counted on the 10 th day of cultivation, and the number of dead seedlings and seedling rate were counted on the 20 th day, and the results are shown in table 5.
TABLE 5F statistics of survival rate and seedling rate of the group cuttings
Note: survival percent is (number of cutting plants-number of dead seedlings at 10 th day)/number of cutting plants 100%; the seedling rate is (number of cutting plants-number of dead seedlings at 20 days)/number of cutting plants 100%.
Comparative example 1
In this comparative example, 470 cuttings were trimmed in the treatment mode of group a in example 1, and comparative scratch treatment was performed as shown in table 6;
TABLE 6 comparative scratch treatment modes
Note: the number of test slips was small because the scratching treatment was difficult in groups G3 and G5.
The rest treatment is the same as the example 1, and the number and the proportion of cuttings with white radicles are counted after the pre-rooting treatment; counting the survival rate on the 10 th day after cuttage; and counting the seedling rate on the 20 th day after cuttage. The results are shown in Table 7.
TABLE 7 statistics of pre-rooting condition, survival rate and seedling rate of control cuttings
Note: 1. in the table, "proportion" means the proportion of the number of cuttings with white young roots to the number of plants;
2. survival percent is (number of cutting plants-number of dead seedlings at 10 th day)/number of cutting plants 100%; the seedling rate is (number of cutting plants-number of dead seedlings at 20 days)/number of cutting plants 100%.
As can be seen from Table 7, the shape of the scratch of the G1 group is not favorable for the protrusion of the root primordium, although the cuttings can survive 100%, the dead seedlings appear in the later period, and the seedling rate is reduced; the G2 group increases the length of the scratch wound, although the root primordium is more favorable to bulge, 5 cuttings with rotten roots appear after the pre-rooting treatment, and the festers are all at the position of the scratch treatment, so the length of the wound subjected to the scratch treatment by the cuttings is not too long, and preferably not more than 4 mm; the corresponding scratch wound is G3 group with 1mm, so that the wound is too small, the difficulty of cutting treatment is increased, the root primordium is difficult to bulge, the survival rate and the seedling rate are relatively reduced, and the length of the wound subjected to the scratch treatment by the cutting is not too short, preferably not less than 2 mm; the G4 group has limited projecting degree of root primordium due to too low scratch density, thus influencing the survival rate of cuttings after cuttage; the G5 group has high scratch density, has root rot phenomenon as the G2 group, is more serious relative to the G2 group, and shows that the ulcerated area is larger, the proportion of the root rot cuttings is increased (accounts for 10 percent), and the cuttings are lost; g6 was not scratched, and its survival rate and seedling rate were significantly reduced compared to A1 group. And the continuous tracking finds that the time of the G1 group, the G3 group and the G4 group is delayed, and the time of the G6 group is delayed to 38 days.
In conclusion, the proper scratching treatment of the cuttings can accelerate the process of projecting the root primordium, improve the survival rate and the seedling rate of the cuttings and obviously shorten the seedling period.
Comparative example 2
The comparative example was set up with tests of groups H1 and H2, each group having 100 cuttings, wherein group H1 differed from group a1 only in that no nutrient solution immersion treatment was performed, group H2 differed from group a1 in that the concentrations of the nutrient solutions used were different, the mass ratio of urea, disodium hydrogen phosphate and calcium sulfate in the nutrient solution of group H2 was 5:2:1, and the total concentration was 0.2 g/L. The cutting results are shown in Table 8.
TABLE 8 influence of nutrient solution on the survival rate and seedling rate of cuttings
Note: survival percent is (number of cutting plants-number of dead seedlings at 10 th day)/number of cutting plants 100%; the seedling rate is (number of cuttage plants-number of dead seedlings in 20 days)/number of cuttage plants is 100%.
As can be seen from table 8, the survival rate of the H1 group in the early stage of the cuttage is still satisfactory without being soaked in the nutrient solution, but the cuttings die a lot in the later stage of the cuttage, and the seedling rate is only 60%, so that the nutrient solution soaking treatment of the invention can significantly improve the seedling rate of the akebia trifoliata cuttings; the concentration of the nutrient solution has a remarkable influence on the survival of the cuttings, the survival rate of the akebia trifoliata cuttings can be remarkably reduced due to the overhigh concentration of the nutrient solution, but the cuttings can basically grow seedlings after survival because the cuttings absorb the nutrient solution.
Comparative example 3
The number of cuttings treated in this comparative example was 100, which was different from the a1 group only in that no pregermination treatment was performed, and the cutting results are shown in table 9.
TABLE 9 Effect of Pre-rooting treatment on shoot survival and seedling Rate
Note: 1. in the table, "proportion" means the proportion of the number of cuttings with white young roots to the number of plants;
2. survival percent is (number of cutting plants-number of dead seedlings at 10 th day)/number of cutting plants 100%; the seedling rate is (number of cutting plants-number of dead seedlings at 20 days)/number of cutting plants 100%.
As can be seen from Table 9, the ratio of the white root protrusion of the cuttings without pre-rooting treatment was significantly reduced, and both the survival rate and the seedling rate were significantly reduced; tracking statistics shows that 53 days are required for the K groups to be cultured until the cutting transplantation can be carried out.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A cutting seedling raising method of akebia trifoliata is characterized by comprising the following steps:
(1) selecting rattans with 1-2 complete stem segments and 1-2 buds as cuttings;
(2) scratching treatment is carried out on stem sections within the range of 2-4 cm above the cutting ends of the cuttings, and only the epidermis is scratched without damaging the phloem;
(3) soaking the cut slips subjected to the scratching treatment in a nutrient solution, and taking out the slips for pre-rooting treatment;
(4) and (4) carrying out cuttage cultivation on the cuttings treated in the step (3) to obtain the akebia trifoliata cuttage seedlings.
2. The cutting seedling method of akebia trifoliata as claimed in claim 1, wherein the scratching treatment is to scratch an X-shaped or a + shaped wound on the epidermis.
3. The cutting seedling method of akebia trifoliata as claimed in claim 2, wherein each scratch wound of the X-shaped or the plus-shaped wound has a length of 2-4 mm.
4. The cutting seedling method of akebia trifoliata as claimed in claim 2 or 3, wherein the density of the X-shaped or the + shaped wounds is 1-4/cm2。
5. The cutting seedling method of akebia trifoliate as described in claim 1, wherein the nutrient solution comprises urea, disodium hydrogen phosphate, calcium sulfate and water, and the total concentration is 0.024-0.05 g/L.
6. The cutting seedling method of akebia trifoliate as described in claim 5, wherein the mass ratio of urea to disodium hydrogen phosphate to calcium sulfate in the nutrient solution is 4-5: 2-3: 1-2.
7. The cutting seedling method of akebia trifoliata as described in claim 1, 5 or 6, wherein the time for soaking in the nutrient solution is 2-5 h.
8. The cutting seedling method of akebia trifoliate as described in claim 1, wherein the pre-rooting treatment is to treat the cuttings in a dark environment with a temperature of 30-35 ℃ and a humidity of 80-90% for 18-24 hours.
9. The cutting seedling method of akebia trifoliate as described in claim 1, wherein the cuttings obtained in step (4) can be transplanted after being cultivated for 20-25 days.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067847A (en) * | 2014-07-24 | 2014-10-01 | 巴东县丰太农业专业合作社 | Cutting seedling culture method of akebia trifoliate |
| CN106508411A (en) * | 2016-12-07 | 2017-03-22 | 广西灏源盛世生物科技有限公司 | Cinnamomum camphora cuttage propagation method |
| CN106613231A (en) * | 2016-12-22 | 2017-05-10 | 阜阳市殿兴农业科技有限公司 | Pawpaw cutting processing method |
| CN106797818A (en) * | 2016-12-29 | 2017-06-06 | 武汉法雅园林集团有限公司 | A kind of method of plane tree scale cuttage |
-
2021
- 2021-03-19 CN CN202110297689.9A patent/CN113057063B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067847A (en) * | 2014-07-24 | 2014-10-01 | 巴东县丰太农业专业合作社 | Cutting seedling culture method of akebia trifoliate |
| CN106508411A (en) * | 2016-12-07 | 2017-03-22 | 广西灏源盛世生物科技有限公司 | Cinnamomum camphora cuttage propagation method |
| CN106613231A (en) * | 2016-12-22 | 2017-05-10 | 阜阳市殿兴农业科技有限公司 | Pawpaw cutting processing method |
| CN106797818A (en) * | 2016-12-29 | 2017-06-06 | 武汉法雅园林集团有限公司 | A kind of method of plane tree scale cuttage |
Non-Patent Citations (1)
| Title |
|---|
| 熊大胜等: "三叶木通扦插生根规律研究", 《湖南文理学院学报(自然科学版)》, vol. 16, no. 04, 30 December 2004 (2004-12-30), pages 59 - 61 * |
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Application publication date: 20210702 Assignee: Hunan Yifang Tianjiang Pharmaceutical Co.,Ltd. Assignor: HUNAN University OF ARTS AND SCIENCE Contract record no.: X2023980036951 Denomination of invention: A Cuttage Seedling Raising Method for Akebia trifoliata Granted publication date: 20221209 License type: Common License Record date: 20230625 |