CN113025484B - Intelligent interactive culture equipment for algae microorganisms - Google Patents

Intelligent interactive culture equipment for algae microorganisms Download PDF

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CN113025484B
CN113025484B CN202110225149.XA CN202110225149A CN113025484B CN 113025484 B CN113025484 B CN 113025484B CN 202110225149 A CN202110225149 A CN 202110225149A CN 113025484 B CN113025484 B CN 113025484B
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CN113025484A (en
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雷小英
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Hunan Tengyang Biotechnology Co ltd
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    • C12M31/00—Means for providing, directing, scattering or concentrating light
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level

Abstract

The invention discloses intelligent interactive algae microorganism culture equipment which comprises a culture pond and a protective cover plate matched with the culture pond in specification, wherein a plurality of balance weight boxes are placed on the inner bottom wall of the culture pond, permanent magnets are fixedly arranged in the balance weight boxes, a plurality of floating mechanisms are arranged in the culture pond, each floating mechanism comprises a light pipe, a mixing pipe and a floating ball, the upper end of each mixing pipe is hermetically connected with the lower end of the corresponding light pipe, each floating ball is fixedly connected with the lower end of the corresponding mixing pipe, a gas storage cavity is formed in each light pipe, and a liquid storage cavity is formed in each mixing pipe. According to the invention, after the difference value of the temperatures measured by the first temperature sensor and the second temperature sensor exceeds a preset value, the floating mechanism is driven to move up and down, the culture solution is fully disturbed, algae microorganisms are promoted to be uniformly distributed, the energy consumption is low, and the electric energy can be effectively saved.

Description

Intelligent interactive culture equipment for algae microorganisms
Technical Field
The invention relates to the technical field of microbial culture, in particular to intelligent interactive culture equipment for algae microorganisms.
Background
The microorganisms are mainly composed of unicellular organisms, the variety of the unicellular organisms is very wide, correspondingly, the application of the microorganisms is also extremely wide, and people can carry out large-scale industrial culture in a culture pond in order to obtain more microbial metabolites.
The algae microorganism can move to the lower part of culture pond along with the deposit of nutrient substance in the culture solution when the stationary culture, consequently the algae microorganism distribution in the culture pond is uneven, local region's the nutrient supply condition that is not enough appears easily, thereby lead to the algae microorganism to die in batches and the area of culture pond is great, if adopt the incessant disturbance culture solution of agitating unit, need consume a large amount of energy, and the culture solution that is close to culture pond edge can not obtain abundant disturbance, still can appear the inhomogeneous condition of algae microorganism distribution, in addition, agitating unit's vortex speed is big, intensity is big, be unfavorable for algae microorganism normal growth, for this reason, we propose an algae microorganism intelligence mutual culture apparatus.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides intelligent interactive culture equipment for algae microorganisms.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides an interactive equipment of cultivateing of algae class microorganism intelligence, is including cultivateing the pond and rather than specification assorted protective cover, a plurality of counter weight boxes have been placed on the interior diapire of cultivation pond, every all the fixed permanent magnet that is equipped with in the counter weight box, be equipped with a plurality of float disturbance mechanisms in the cultivation pond, every float disturbance mechanism all includes light pipe, hybrid tube and floater, every the upper end of hybrid tube all with the lower extreme sealing connection of corresponding light pipe, every the floater all with the lower extreme fixed connection of corresponding hybrid tube, every the gas storage chamber has all been seted up in the light pipe, every the stock solution chamber has all been seted up in the hybrid tube, and the corresponding gas storage chamber in position is linked together with the stock solution chamber, every the lower extreme in stock solution chamber all communicates with the cultivation pond through the round hole.
In the interactive equipment of cultivateing of above-mentioned alga class microorganism intelligence, every the top of light pipe all is equipped with the air inlet that is linked together with corresponding gas storage chamber, and installs pressure regulating mechanism in the air inlet, every pressure regulating mechanism all includes micro-fan and second solenoid valve, every all sealed slidable mounting has a sliding plug in the gas storage chamber, every all install first temperature sensor on the lateral wall of light pipe.
In the interactive equipment of cultivateing of above-mentioned alga class microorganism intelligence, every annular outage groove has all been seted up in the lateral wall of hybrid tube, and the intercommunication has the shunt tubes between outage groove and the stock solution chamber that corresponds, every the first solenoid valve is all installed in shunt tubes and stock solution chamber junction, every a plurality of outage have all been seted up on the lateral wall of outage groove, and a plurality of outage that are located on same outage groove are spiral shape align to grid.
In the intelligent interactive algae microbe culturing apparatus, each floating ball is provided with an electromagnet and a mainboard, the lower end of each floating ball is provided with a second temperature sensor, and the density of each floating ball is less than 1g/cm 3 。
In above-mentioned algae class microorganism intelligence interactive incubation equipment, it is provided with the insulating layer to encircle on the lateral wall of culture pond, around being equipped with heating coil between insulating layer and the culture pond, the outside fixed mounting of culture pond has automatically controlled screen, and is a plurality of the mainboard all pairs through bluetooth module and automatically controlled screen.
In above-mentioned mutual cultivation equipment of algae microorganism intelligence, be equipped with fluid infusion pipe and the intake pipe that extends to in the culture pond on the protection cover plate, install gas detection appearance and level sensor on the inside wall of culture pond.
Compared with the prior art, the invention has the beneficial effects that:
1. through setting up counter weight box, permanent magnet and electro-magnet, can adjust the position of floating the mechanism through the position that changes the counter weight box, make its effect better, the direction in the magnetic field that the electro-magnet produced is opposite with the magnetic field direction of permanent magnet, and both magnetism attract mutually, make to float and disturb the mechanism and keep vertical, reduce and float the shared space of mechanism.
2. After the difference value of the temperatures measured by the first temperature sensor and the second temperature sensor exceeds a preset value, the mainboard controls the micro fan, the first electromagnetic valve and the second electromagnetic valve to be opened, air is introduced into the air storage cavity, so that the volume of the liquid storage cavity is compressed, the culture liquid in the liquid storage cavity is sprayed out through the liquid discharge hole, and the flowing of the surrounding culture liquid and the communication of algae microorganisms are promoted.
3. When the air in the air storage cavity is gradually increased and the culture solution in the liquid storage cavity is gradually reduced, the weight of the floating interference mechanism is reduced, the buoyancy is increased, so that the floating interference mechanism can float upwards, the turbulent flow range after the culture solution is sprayed out is enlarged, the algae microorganisms in the lower layer area are brought to the upper layer of the culture solution, the density of local algae microorganisms is reduced, and the algae microorganisms are promoted to grow faster.
4. The main board controls the periodic change direction of the current flowing through the electromagnet, so that the acting force of the magnetic field between the electromagnet and the permanent magnet is changed repeatedly, and the floating mechanism rotates simultaneously in the upward movement process, thereby improving the flowing speed of the culture solution discharged from the liquid discharge hole and enlarging the disturbed flow range.
5. Only when the difference value of the temperatures measured by the first temperature sensor and the second temperature sensor is reduced to a set range, the floating mechanism stops moving up and down, and in the process, the culture solution at each position of the culture pond can be fully disturbed, so that the algae microorganisms are uniformly distributed, the energy consumption is low, and the electric energy can be effectively saved.
Drawings
FIG. 1 is a schematic diagram of an external structure of a floating mechanism in an intelligent interactive algae microorganism culture apparatus according to the present invention;
FIG. 2 is a schematic diagram of an internal structure of an intelligent interactive algae microorganism cultivation apparatus according to the present invention;
FIG. 3 is an enlarged view of FIG. 2 at A;
FIG. 4 is a schematic diagram of an internal structure of a floating mechanism in the intelligent interactive algae microorganism culture apparatus according to the present invention;
FIG. 5 is an enlarged view of FIG. 4 at B;
FIG. 6 is an enlarged view at C of FIG. 4;
FIG. 7 is a schematic view of a part of the structure of a floating disturbance mechanism in the intelligent interactive algae microorganism culture apparatus according to the present invention;
fig. 8 is a simplified schematic diagram of stress analysis of a floating mechanism in the intelligent interactive culture apparatus for algae microorganisms according to the present invention.
In the figure: 1 light pipe, 101 luminous layer, 102 gas storage cavity, 2 mixing tubes, 201 liquid discharge hole, 202 liquid storage cavity, 203 liquid discharge groove, 3 floating balls, 4 first temperature sensor, 5 second temperature sensor, 6 culture pond, 7 protective cover plate, 8 liquid supply pipe, 9 air inlet pipe, 10 gas detector, 11 liquid level sensor, 12 heat insulation layer, 13 heating coil, 14 electric control screen, 15 balance weight box, 16 permanent magnet, 17 electromagnet, 18 main board, 19 shunt pipe, 20 sliding plug, 21 first electromagnetic valve, 22 air inlet, 23 micro fan, 24 second electromagnetic valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Examples
Referring to fig. 1-8, an interactive cultivation equipment of algae microorganism intelligence, including culture pond 6 and rather than specification assorted protective cover 7, protective cover 7 can avoid the microorganism in the culture pond 6 to receive the interference of miscellaneous fungus, a plurality of counter weight boxes 15 have been placed on the interior diapire of culture pond 6, all be fixed with permanent magnet 16 in every counter weight box 15, counter weight box 15 is not fixed with the interior diapire of culture pond 6, conveniently change the position of permanent magnet 16, be equipped with a plurality of float mechanism in the culture pond 6, every float mechanism all includes light pipe 1, hybrid tube 2 and floater 3, the upper end of every hybrid tube 2 all is connected with the lower extreme sealing of corresponding light pipe 1, every floater 3 all is connected with the lower extreme fixed connection of corresponding hybrid tube 2, gas storage cavity 102 has all been seted up in every light pipe 1, liquid storage cavity 202 has all been seted up in every hybrid tube 2, and the gas storage cavity 102 that the position corresponds is linked together with liquid storage cavity 202, gas storage cavity 102 equals with liquid storage cavity 202, and the junction is smooth processing, the lower extreme of every liquid storage cavity 202 all passes through the round hole and cultivates 6, the liquid storage cavity communicates through static culture liquid storage when meeting the mechanism, float mechanism.
In the invention, the top end of each light pipe 1 is provided with an air inlet 22 communicated with the corresponding air storage cavity 102, a pressure regulating mechanism is arranged in the air inlet 22, each pressure regulating mechanism comprises a micro fan 23 and a second electromagnetic valve 24, the second electromagnetic valve 24 is used for controlling the conduction or the closure of the air inlet 22, a sliding plug 20 is hermetically and slidably arranged in each air storage cavity 102, the sliding plug 20 can freely slide in the vertical direction, the outer side wall of each light pipe 1 is provided with a first temperature sensor 4, and the light-emitting layer 101 is adhered to the outer part of each light pipe 1, so that the light-emitting layer 101 can attract algae microorganisms to move to the upper part of a culture solution because some algae microorganisms have phototaxis, and the phenomenon of uneven distribution of the algae microorganisms is improved.
In the invention, the side wall of each mixing pipe 2 is internally provided with an annular liquid discharge groove 203, a shunt pipe 19 is communicated between the liquid discharge groove 203 and the corresponding liquid storage cavity 202, the shunt pipe 19 comprises a main pipe and a plurality of branch pipes communicated with the main pipe, the main pipe is communicated with the liquid storage cavities 202, the branch pipes are uniformly distributed in the circumferential direction and are communicated with the liquid discharge grooves 203, the joint of each shunt pipe 19 and the liquid storage cavity 202 is provided with a first electromagnetic valve 21, when the first electromagnetic valve 21 is closed, liquid cannot enter the liquid storage cavity 202 from the liquid discharge grooves 203, the outer side wall of each liquid discharge groove 203 is provided with a plurality of liquid discharge holes 201, and the plurality of liquid discharge holes 201 on the same liquid discharge groove 203 are uniformly arranged in a spiral shape, thereby enlarging the action range.
In the invention, each floating ball 3 is internally provided with an electromagnet 17 and a main board 18, the lower end of each floating ball 3 is provided with a second temperature sensor 5, and the density of each floating ball 3 is less than 1g/cm 3 The buoyancy that the mechanism received is disturbed in the improvement, increases and floats the stability that disturbs the mechanism, still is equipped with miniature lithium cell in the floater 3, and miniature lithium cell provides the electric energy for floating the return circuit that disturbs in the mechanism, and the back is exhausted to miniature lithium cell's electric quantity, inhales the method energy storage that charges through magnetism, need not set up the socket on 3 surfaces of floater, guarantees the leakproofness of floater 3, improves the security that floats the mechanism.
According to the invention, the outer side wall of the culture pond 6 is provided with the heat insulation layer 12 in a surrounding mode, the heating coil 13 is arranged between the heat insulation layer 12 and the culture pond 6 in a surrounding mode, the heating coil 13 is used for improving the temperature of the culture pond 6, the heat insulation layer 12 plays a role in heat insulation and can prevent the heating coil 13 from being damaged by external force, the electronic control screen 14 is fixedly installed outside the culture pond 6, the plurality of main boards 18 are all matched with the electronic control screen 14 through the Bluetooth module, and the temperatures and the difference values measured by the first temperature sensor 4 and the second temperature sensor 5 can be transmitted to the electronic control screen 14 in real time.
According to the invention, a liquid supplementing pipe 8 and an air inlet pipe 9 which extend into the culture pond 6 are arranged on the protective cover plate 7 and are used for supplementing the culture liquid and oxygen in the culture pond 6, a gas detector 10 and a liquid level sensor 11 are arranged on the inner side wall of the culture pond 6, the gas detector 10 is mainly used for detecting the content of oxygen, and the liquid level sensor 11 is mainly used for detecting the consumption of the culture liquid.
The invention can be illustrated by the following operating modes: the floating mechanism is placed in the culture pond 6 and is positioned above the corresponding counterweight box 15, the direction of a magnetic field generated by the electromagnet 17 is opposite to that of a magnetic field of the permanent magnet 16, and the two magnetic fields attract each other, so that the floating mechanism is kept vertical;
the mechanism of floating and disturbing can be regarded as a whole, after placing the mechanism of floating and disturbing to culture pond 6, mutual appeal between electro-magnet 17 and the permanent magnet 16 can be regarded as to have a rope to be tied in the bottom of floating and disturbing the mechanism to the bottom of rope and the interior diapire fixed connection of culture pond 6, to floating and disturbing the mechanism, when moment that its buoyancy produced is greater than gravity moment, float and disturb the mechanism and can be in vertical balanced state, simplify the model to the back that fig. 8 shows, can know according to the relation between mechanics knowledge analysis buoyancy and the gravity:
Figure BDA0002955544920000071
wherein h is the distance of the floating mechanism immersing in the liquid level, and L is the vertical distance between the top and the bottom of the floating mechanism;
assuming that the average cross-sectional area of the floating mechanism is S:
F＝ρ 1 gSh；
G＝ρ 2 gSL；
where ρ is 1 Average density of culture broth, p 2 Is the air density, g is the acceleration of gravity;
the formula above can be combined to obtain:
Figure BDA0002955544920000081
i.e., the depth of the culture solution should be
Figure BDA0002955544920000082
Detecting the depth change of the culture solution by a liquid level sensor 11, and if the height of the culture solution is reduced, supplementing the culture solution into the culture pond 6 by a liquid supplementing pipe 8;
during static culture, the algae microorganisms move to the lower part of the culture pond 6 along with the deposition of nutrient substances in the culture solution, so that the algae microorganisms contained in the upper half part of the culture solution in the culture pond 6 are few, and the algae microorganisms can generate heat in the metabolic process, so that the upper part and the lower part of the culture solution have certain temperature difference;
when the temperature difference detected by the first temperature sensor 4 and the second temperature sensor 5 reaches a certain value, the main board 18 controls the micro fan 23, the first electromagnetic valve 21 and the second electromagnetic valve 24 to be opened, air is introduced into the air storage cavity 102 (the height of the air inlet 22 is always higher than that of the culture solution), under the action of pressure, the sliding plug 20 slides downwards to extrude the culture solution in the liquid storage cavity 202 into the liquid discharge tank 203 and then is discharged out through the liquid discharge hole 201, and the track of the discharged culture solution is spiral, as shown in fig. 7, the flow of the surrounding culture solution and the communication of algae microorganisms can be promoted;
the diameter of the circular hole through which the liquid storage cavity 202 is communicated with the culture pond 6 is small, when the sliding plug 20 slides downwards at a high speed, the pressure in the liquid storage cavity 202 is high, and according to fluid mechanics, the culture solution is dispersed into the liquid discharge tank 203 through the shunt pipe 19 preferentially;
meanwhile, the main board 18 controls the periodic changing direction of the current flowing through the electromagnet 17, so that the magnetic attraction between the electromagnet 17 and the permanent magnet 16 is changed into a magnetic repulsion force, and then the magnetic repulsion force is changed into a magnetic attraction force, and the magnetic attraction force is changed repeatedly, because the air in the air storage cavity 102 is gradually increased, the culture solution in the liquid storage cavity 202 is gradually reduced, the weight of the floating mechanism is reduced, the buoyancy is increased, the floating mechanism can float upwards, and under the comprehensive action of a magnetic field, the floating mechanism can rotate, so that the flowing speed of the culture solution discharged from the liquid discharge hole 201 is increased, and the turbulent flow range is enlarged;
when the second temperature sensor 5 rises along with the floating ball 3, the temperature of the culture solution detected by the second temperature sensor is reduced, so that the difference value of the temperatures measured by the first temperature sensor 4 and the second temperature sensor 5 is reduced, when the difference value is smaller than a certain value, the mainboard 18 controls the micro fan 23 to rotate reversely, air in the air storage cavity 102 is exhausted, the sliding plug 20 slides upwards under the action of pressure, the culture solution in the liquid storage cavity 202 increases again, the weight and the buoyancy of the floating mechanism are increased and reduced, so that the floating mechanism sinks again until the sliding plug 20 resets, the mainboard 18 controls the first electromagnetic valve 21, the micro fan 23 and the second electromagnetic valve 24 to be closed, the direction of the current passing through the electromagnet 17 is not changed any more, and the floating mechanism recovers stability;
if the difference value of the temperatures measured by the first temperature sensor 4 and the second temperature sensor 5 still exceeds a preset value, the floating interference mechanism repeats the process until the temperature difference is reduced to a preset range, and in the process of up-and-down movement of the floating interference mechanism, the floating interference mechanism not only can realize the interference of the culture solution and increase the communication of the algae microorganisms, but also can convey the algae microorganisms in the lower area to the upper part of the culture solution, so that the density of local algae microorganisms is reduced, and the algae microorganisms are promoted to grow faster.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (3)

1. The intelligent interactive algae microorganism culture equipment comprises a culture pond (6) and a protective cover plate (7) matched with the culture pond in specification, and is characterized in that a plurality of weight boxes (15) are placed on the inner bottom wall of the culture pond (6), a permanent magnet (16) is fixedly arranged in each weight box (15), a plurality of floating mechanisms are arranged in the culture pond (6), each floating mechanism comprises a light pipe (1), a mixing pipe (2) and a floating ball (3), the upper end of each mixing pipe (2) is hermetically connected with the lower end of the corresponding light pipe (1), each floating ball (3) is fixedly connected with the lower end of the corresponding mixing pipe (2), a gas storage cavity (102) is formed in each light pipe (1), a liquid storage cavity (202) is formed in each mixing pipe (2), the gas storage cavities (102) corresponding in position are communicated with the liquid storage cavities (202), and the lower end of each liquid storage cavity (202) is communicated with the culture pond (6) through a round hole;
the top end of each light pipe (1) is provided with an air inlet (22) communicated with a corresponding air storage cavity (102), a pressure regulating mechanism is installed in each air inlet (22), each pressure regulating mechanism comprises a micro fan (23) and a second electromagnetic valve (24), a sliding plug (20) is installed in each air storage cavity (102) in a sealing and sliding mode, and a first temperature sensor (4) is installed on the outer side wall of each light pipe (1);
an annular liquid discharge groove (203) is formed in the side wall of each mixing pipe (2), flow dividing pipes (19) are communicated between the liquid discharge grooves (203) and the corresponding liquid storage cavities (202), a first electromagnetic valve (21) is mounted at the joint of each flow dividing pipe (19) and the corresponding liquid storage cavity (202), a plurality of liquid discharge holes (201) are formed in the outer side wall of each liquid discharge groove (203), and the liquid discharge holes (201) in the same liquid discharge groove (203) are uniformly arranged in a spiral shape;
an electromagnet (17) and a main board (18) are arranged in each floating ball (3), a second temperature sensor (5) is arranged at the lower end of each floating ball (3), and the density of each floating ball (3) is smaller than 1g/cm 3 。
2. The interactive intelligent algae microorganism culturing apparatus according to claim 1, wherein a heat insulating layer (12) is arranged around the outer side wall of the culture tank (6), a heating coil (13) is arranged between the heat insulating layer (12) and the culture tank (6), an electric control screen (14) is fixedly mounted outside the culture tank (6), and the plurality of main boards (18) are matched with the electric control screen (14) through a bluetooth module.
3. The interactive intelligent algae microorganism culturing apparatus according to claim 1, wherein the protective cover plate (7) is provided with a liquid supplementing pipe (8) and an air inlet pipe (9) which extend into the culture pond (6), and the inner side wall of the culture pond (6) is provided with a gas detector (10) and a liquid level sensor (11).
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