CN216155883U

CN216155883U - Stem cell culture device

Info

Publication number: CN216155883U
Application number: CN202122151800.1U
Authority: CN
Inventors: 田爱鹏; 杨远方; 亢星亮; 吴佳蔓
Original assignee: Henan Intercell Biology Technology Co ltd
Current assignee: Henan Intercell Biology Technology Co ltd
Priority date: 2021-09-07
Filing date: 2021-09-07
Publication date: 2022-04-01
Anticipated expiration: 2031-09-07

Abstract

The utility model relates to a stem cell culture device, which comprises a supporting bottom plate and two vertical plates, wherein a liquid storage tank, a mounting plate and a culture tank are sequentially arranged between the two vertical plates from top to bottom, a controller is arranged at the top of the mounting plate, a top cover is connected to the top end of the culture tank in a threaded manner, a T-shaped supporting block is fixed on the top cover, a liquid supplementing pipe is communicated between the liquid storage tank and the culture tank, the bottom end of the liquid supplementing pipe sequentially penetrates through the mounting plate and the top cover and extends to the lower part inside the culture tank, an electromagnetic valve is arranged on the liquid supplementing pipe, a sliding sleeve is arranged above the inside of the culture tank, a sliding rod is connected in the sliding sleeve in a sliding manner, the top end of the sliding rod extends to the upper part of the top cover and is fixed with a pressing plate, the pressing plate is positioned above the T-shaped supporting block, a contact switch is arranged at the top of the T-shaped supporting block, a floating ball assembly is fixed at the bottom end of the sliding rod, and the electromagnetic valve and the contact switch are electrically connected with the controller. Simple structure, convenient operation can need not artifical fluid infusion to the inside automatic cell culture liquid that adds of culture tank, reduces the contaminated probability of stem cell.

Description

Stem cell culture device

Technical Field

The utility model belongs to the technical field of biology, and particularly relates to a stem cell culture device.

Background

Stem cells are a type of pluripotent cells with the ability to self-renew, self-replicate. The stem cells can be classified into totipotent stem cells, pluripotent stem cells and unipotent stem cells according to different differentiation potentials. The study of stem cells has become the most attractive field in natural science due to its multipotentiality, unlimited division, proliferation, etc. The perfect improvement of stem cell theory and the rapid development of technology also provide a new research direction for the fields of disease treatment, biomedicine and the like. The stem cell in vitro culture technology is a key factor for realizing safe and effective amplification of stem cells, and the stem cells cultured in vitro can be used in the fields of life science research, drug screening test, clinical application research and the like. The existing stem cell culture device cannot automatically add culture solution, needs frequent culture solution adding into the culture device by operators, is complex in operation, increases the workload of the operators, and can not well meet the use requirement if the operators are improper and the stem cells are easily polluted in the process of frequently adding the culture solution.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a stem cell culture device, which is used for solving the technical problem that a stem cell culture device in the prior art cannot automatically add a cell culture solution.

In order to solve the technical problems, the technical scheme adopted by the utility model is as follows: the stem cell culture device comprises a horizontally arranged supporting bottom plate, wherein two symmetrically arranged vertical plates are fixed on two sides of the supporting bottom plate, a liquid storage tank, a mounting plate and a culture tank are sequentially arranged between the two vertical plates from top to bottom, the liquid storage tank and the culture tank are detachably connected with the vertical plates, two ends of the mounting plate are respectively fixed on the inner sides of the two vertical plates, and a controller is arranged at the top of the mounting plate; cultivate the top threaded connection of jar and have the top cap, be fixed with T type supporting shoe on the top cap, the intercommunication has the moisturizing pipe between liquid storage pot and the cultivation jar, the mounting panel is run through in proper order to the bottom of moisturizing pipe, the top cap extends to the inside below of cultivation jar, be equipped with the solenoid valve on the moisturizing pipe, the inside top of cultivation jar is provided with the sliding sleeve, the inside sliding connection of sliding sleeve has the slide bar of vertical setting, the top of slide bar extends to the top of top cap and the clamp plate that fixed connection has the level to set up, the clamp plate is located the top of T type supporting shoe, the top of T type supporting shoe is provided with the contact switch, the bottom fixedly connected with who slides the slide bar floats the ball subassembly, the solenoid valve, the contact switch all is connected with the controller electricity.

Preferably, the side wall of the culture tank is of a double-layer structure with a hollow interior, the double-layer structure is an outer wall and an inner wall, the inner wall is spirally wound with a heat source pipe, and the upper end and the lower end of the heat source pipe both penetrate through the outer wall and extend to the outside of the culture tank.

Preferably, the top of the mounting plate is fixedly connected with a carbon dioxide gas storage tank, a compressed air gas storage tank and a liquid storage bottle which are arranged at intervals, sterilizing water is stored in the liquid storage bottle, the top of the carbon dioxide gas storage tank and the top of the compressed air gas storage tank are respectively communicated with a first air supply hose and a second air supply hose, a first electromagnetic flow regulating valve is arranged on the first air supply hose, a second electromagnetic flow regulating valve is arranged on the second air supply hose, the first electromagnetic flow regulating valve and the second electromagnetic flow regulating valve are electrically connected with the controller, a three-way connecting pipe is communicated between the first air supply hose and the second air supply hose, the other end of the three-way connecting pipe is communicated with a vertically downward air inlet main pipe, a one-way valve is arranged on the air inlet main pipe, the bottom end of the air inlet main pipe sequentially penetrates through the mounting plate and the top cover and extends to the upper part inside the culture tank, an exhaust hose communicated with the inside of the culture tank is fixed on the top cover, the other end of the exhaust hose penetrates through the mounting plate and is communicated with an L-shaped pipe, the bottom end of the vertical section of the L-shaped pipe is positioned at the lower part of the liquid level of the sterilized water, and a carbon dioxide concentration sensor for feeding back signals to the controller is arranged below the top cover.

Preferably, the bottom end of the air inlet header pipe is fixedly connected with an air inlet disc which is horizontally arranged, the air inlet disc is a circular disc which is hollow inside, a vent hole is formed in the middle of the top of the air inlet disc, the bottom end of the air inlet header pipe is fixed in the middle of the top of the air inlet disc and covers the vent hole, and a plurality of air inlet holes which are uniformly arranged at intervals are formed in the bottom of the air inlet disc.

Preferably, the floater assembly comprises a cross-shaped mounting frame, the cross-shaped mounting frame is fixed at the bottom end of the sliding rod, and floating balls are fixedly connected to four end portions of the cross-shaped mounting frame.

Preferably, the top of cultivateing the jar is provided with sampling component, sampling component is including setting up the bellows in the top cap top, and the bellows is inside hollow structure, the vertical sampling tube that sets up downwards of bottom fixedly connected with of bellows, and the sampling tube is linked together with the bellows, and the bottom of sampling tube runs through the top cap and extends to the inside below of cultivateing the jar, is located fixedly connected with drain pipe on the sampling tube of cultivateing jar top, and the drain pipe downward sloping sets up and is linked together with the sampling tube, is equipped with the stop valve on the drain pipe.

Preferably, the lower part of the liquid supplementing pipe is attached to the side wall inside the culture tank, the section of the bottom of the liquid supplementing pipe is an inclined plane, and the lowest end of the bottom of the liquid supplementing pipe is attached to the side wall inside the culture tank.

Preferably, the top of the liquid storage tank is in threaded connection with an upper cover, the middle position of the top of the upper cover is communicated with a vertically and upwardly arranged feeding pipe, and the top of the feeding pipe is in threaded connection with a pipe cap.

The utility model has the beneficial effects that: simple structure, convenient operation, through setting up the liquid storage pot, the culture tank, the fluid infusion pipe, the solenoid valve, the slide bar, the floater subassembly, clamp plate and contact switch, in the process of stem cell culture, along with the decline of the liquid level in the culture tank, under the effect of gravity, the position of floater subassembly descends, the floater subassembly drives the slide bar in proper order, the clamp plate moves down, when the bottom of clamp plate touches the contact switch, the contact switch transmits the signal to the controller, the controller controls the solenoid valve to open, the cell culture fluid in the liquid storage pot enters the culture tank through the fluid infusion pipe, in time supplement the cell culture fluid in the culture tank, along with the rise of the liquid level in the culture tank, the floater subassembly also can follow the rise of water level and rise, the floater subassembly drives the slide bar in proper order, the clamp plate moves up, after the bottom of clamp plate leaves the contact switch, the contact switch transmits the signal to the controller, the controller controls the electromagnetic valve to close and stops supplementing the cell culture solution into the culture tank. The cell culture fluid can be automatically supplemented into the culture tank according to the use requirement, the automation degree is high, frequent cell fluid addition of workers is avoided, the work load is reduced, the whole fluid supplementing process is carried out in a sealed device, the culture tank does not need to be opened, the stem cells are prevented from contacting with the external environment in the fluid supplementing process, and the probability of stem cell pollution is reduced.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view showing the internal structure of a culture tank;

FIG. 3 is a schematic view showing the structure of the connection of a heat source tube to a culture tank;

FIG. 4 is a first schematic structural diagram of an air inlet disc;

FIG. 5 is a second schematic structural view of an air intake disc;

fig. 6 is a schematic structural view of the float ball assembly.

Reference numerals: 1-supporting bottom plate, 2-vertical plate, 3-liquid storage tank, 4-mounting plate, 5-culture tank, 6-C type clamping plate, 7-positioning block, 8-upper cover, 9-feeding pipe, 10-pipe cap, 11-controller, 12-top cover, 13-T type supporting block, 14-liquid supplementing pipe, 15-electromagnetic valve, 16-sliding sleeve, 17-sliding rod, 18-mounting rod, 19-supporting rod, 20-pressing plate, 21-contact switch, 22-outer wall, 23-inner wall, 24-heat source pipe, 25-carbon dioxide gas storage tank, 26-compressed air gas storage tank, 27-liquid storage bottle, 28-first air supply hose, 29-second air supply hose, 30-first electromagnetic flow regulating valve, 31-second electromagnetic flow regulating valve, 32-three-way connecting pipe, 33-air inlet main pipe, 34-one-way valve, 35-air outlet hose, 36-L type pipe, 37-carbon dioxide concentration sensor, 38-air inlet disk, 39-vent hole, 40-air inlet hole, 41-cross mounting frame, 42-floating ball, 43-corrugated pipe, 44-sampling tube, 45-liquid outlet pipe, 46-stop valve.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clear, embodiments of the present invention are described in further detail below with reference to the accompanying drawings.

As shown in figures 1 and 2, the utility model comprises a horizontally arranged support base plate 1, wherein two sides of the support base plate 1 are fixed with symmetrically arranged vertical plates 2, a liquid storage tank 3, a mounting plate 4 and a culture tank 5 are sequentially arranged between the two vertical plates 2 from top to bottom, and the liquid storage tank 3 and the culture tank 5 are detachably connected with the vertical plates 2. The both sides of liquid storage pot 3 and the equal fixedly connected with symmetry in both sides of culture tank 5 set up C type cardboard 6, and the inboard fixedly connected with of riser 2 and the locating piece 7 that C type cardboard 6 one-to-one set up all set up threaded hole on locating piece 7 and the C type cardboard 6, and C type cardboard 6 is fixed on locating piece 7 through the bolt that passes the screw hole. The liquid storage tank 3 and the culture tank 5 are convenient to install and disassemble, and are convenient to clean and overhaul. The top threaded connection of liquid storage pot 3 has upper cover 8, and the intermediate position intercommunication at upper cover 8 top has the vertical charge-in tube 9 that upwards sets up, and the top threaded connection of charge-in tube 9 has pipe cap 10. The constant-temperature cell culture solution is added into the liquid storage tank 3 through the feeding pipe 9, and the pipe cap 10 is covered after the feeding is finished, so that the cell culture solution is prevented from being polluted. The inner wall of the liquid storage tank 3 can be coated with a heat-insulating layer, so that the cell culture solution stored in the liquid storage tank 3 is always kept in a constant temperature range, and the growth and the propagation of stem cells are facilitated. The both ends of mounting panel 4 are fixed respectively in the inboard of two riser 2, and the top of mounting panel 4 is provided with controller 11. The top threaded connection of culture tank 5 has top cap 12, is fixed with T type supporting shoe 13 on the top cap 12, and the intercommunication has fluid infusion pipe 14 between liquid storage pot 3 and the culture tank 5, and mounting panel 4, top cap 12 are run through in proper order and extend to the inside below of culture tank 5 in the bottom of fluid infusion pipe 14, are equipped with solenoid valve 15 on the fluid infusion pipe 14, and the inside top of culture tank 5 is provided with sliding sleeve 16, and the inside sliding connection of sliding sleeve 16 has the slide bar 17 of vertical setting. The bottom of top cap 12 is fixed with vertical installation pole 18 that sets up downwards, and the lower part of installation pole 18 is close to one side of sliding sleeve 16 and is fixed with two bracing pieces 19 that the interval set up from top to bottom, and bracing piece 19 level sets up on installation pole 18, and one side and the bracing piece 19 fixed connection of sliding sleeve 16. The sliding rod 17 is guided and limited by the sliding sleeve 16, so that the sliding rod 17 is prevented from deviating during up-and-down movement. The top end of the sliding rod 17 extends to the upper part of the top cover 12 and is fixedly connected with a horizontally arranged pressing plate 20, the pressing plate 20 is located above the T-shaped supporting block 13, the top of the T-shaped supporting block 13 is provided with a contact switch 21, the bottom end of the sliding rod 17 is fixedly connected with a floating ball component, the electromagnetic valve 15 and the contact switch 21 are both electrically connected with the controller 11, and the contact switch 21 is used for controlling the opening and closing of the electromagnetic valve 15.

Preferably, as shown in fig. 2 and 3, the side wall of the culture tank 5 has a double-layer structure with a hollow interior, namely an outer wall 22 and an inner wall 23, the inner wall 23 is spirally wound with a heat source pipe 24, and both upper and lower ends of the heat source pipe 24 penetrate through the outer wall 22 and extend to the outside of the culture tank 5. Constant-temperature clean water is introduced into the heat source pipe 24, the heat source pipe 24 is spirally wound on the inner wall 23, and a constant-temperature heat source is introduced into the heat source pipe 24, so that the inside of the culture tank 5 is heated more uniformly, the overheating phenomenon is avoided, the constant temperature inside the culture tank 5 can be kept, and the growth and the propagation of stem cells are facilitated.

Preferably, as shown in fig. 1, a carbon dioxide gas storage tank 25, a compressed air gas storage tank 26 and a liquid storage bottle 27 are fixedly connected to the top of the mounting plate 4 at intervals, the carbon dioxide gas storage tank 25 stores sterilized compressed carbon dioxide gas, the compressed air gas storage tank 26 stores sterilized compressed air, the liquid storage bottle 27 stores sterilized water, the top of the carbon dioxide gas storage tank 25 and the top of the compressed air gas storage tank 26 are respectively communicated with a first air supply hose 28 and a second air supply hose 29, the first air supply hose 28 is provided with a first electromagnetic flow control valve 30, the second air supply hose 29 is provided with a second electromagnetic flow control valve 31, the first electromagnetic flow control valve 30 and the second electromagnetic flow control valve 31 are electrically connected to the controller 11, a three-way connecting pipe 32 is communicated between the first air supply hose 28 and the second air supply hose 29, the other end of the three-way connecting pipe 32 is communicated with a vertically downward arranged air inlet manifold 33, and a one-way valve 34 is arranged on the air inlet manifold 33 to prevent the gas from flowing back into the carbon dioxide gas storage tank 25 and the compressed air gas storage tank 26. As shown in fig. 2, the bottom end of the air inlet manifold 33 penetrates the mounting plate 4 and the top cover 12 in sequence and extends to the upper part inside the culture tank 5, an exhaust hose 35 communicating with the inside of the culture tank 5 is fixed on the top cover 12, the other end of the exhaust hose 35 penetrates the mounting plate 4 and is communicated with an L-shaped pipe 36, the bottom end of the vertical section of the L-shaped pipe 36 is positioned at the lower part of the liquid level of the sterilized water, and a carbon dioxide concentration sensor 37 for feeding back a signal to the controller 11 is arranged below the top cover 12. The mixture of carbon dioxide and air after sterilization treatment enters the culture tank 5 through the air inlet main pipe 33, waste gas generated in the cell growth and metabolism process sequentially enters the liquid storage bottle 27 through the exhaust hose 35 and the L-shaped pipe 36, the end part of the vertical section of the L-shaped pipe 36 is sealed by sterilized water, and the L-shaped pipe 36 is prevented from being directly exposed to the external environment and being polluted. Provides a continuous fresh and clean gas atmosphere in the culture tank 5 and provides a good growth environment for stem cells. During the culture process of the stem cells, the optimal gas atmosphere is 5% of carbon dioxide in volume fraction and 95% of air in volume fraction, therefore, by providing the carbon dioxide concentration sensor 37, the carbon dioxide concentration sensor 37 monitors the concentration of carbon dioxide in the culture tank 5 in real time, when the concentration of carbon dioxide is higher than the set value of 5%, the carbon dioxide concentration sensor 37 transmits a signal to the controller 11, the controller 11 controls the first electromagnetic flow control valve 30 and the second electromagnetic flow control valve 31 to reduce the flow rate of carbon dioxide supplied to the inside of the culture tank 5 and increase the flow rate of air, when the concentration of carbon dioxide is lower than the set value of 5%, the carbon dioxide concentration sensor 37 transmits a signal to the controller 11, and the controller 11 regulates and controls the first electromagnetic flow regulating valve 30 and the second electromagnetic flow regulating valve 31 to increase the flow of carbon dioxide delivered to the interior of the culture tank 5 and simultaneously reduce the flow of air; when the concentration of the carbon dioxide is restored to 5%, the controller 11 regulates the first electromagnetic flow control valve 30 and the second electromagnetic flow control valve 31 again, so that both are restored to the initial setting state. The automation degree is high, so that the gas atmosphere in the culture tank 5 is always kept to be 5% of carbon dioxide and 95% of air by volume fraction, and a good environment is provided for the growth and propagation of stem cells.

Preferably, as shown in fig. 2, the bottom end of the intake manifold 33 is fixedly connected with a horizontally arranged intake disc 38, as shown in fig. 4 and 5, the intake disc 38 is a circular disc with a hollow interior, a vent hole 39 is formed in the middle of the top of the intake disc 38, the bottom end of the intake manifold 33 is fixed in the middle of the top of the intake disc 38 and covers the vent hole 39, and a plurality of intake holes 40 are formed in the bottom of the intake disc 38 and are uniformly spaced. After entering the air inlet disc 38, the gas delivered by the air inlet manifold 33 is dispersed and output outwards through the air inlet holes 40, so that on one hand, the impact of the gas on stem cells is reduced, and on the other hand, the contact area between the clean air and the culture solution in the culture tank 5 is increased, which is beneficial to the growth of the stem cells.

Preferably, as shown in fig. 6, the float assembly includes a cross-shaped mounting bracket 41, the cross-shaped mounting bracket 41 is fixed to the bottom end of the slide bar 17, and a float 42 is fixedly connected to each of four ends of the cross-shaped mounting bracket 41. Through setting up a plurality of floater 42, can increase the buoyancy that the slide bar 17 bottom received, make its reaction more sensitive.

Preferably, as shown in fig. 1 and 2, the top of culture tank 5 is provided with sampling assembly, sampling assembly is including setting up bellows 43 in top cap 12 top, bellows 43 is inside hollow structure, the material of bellows 43 is elastic rubber, the vertical sampling tube 44 that sets up downwards of bottom fixedly connected with of bellows 43, sampling tube 44 is linked together with bellows 43, top cap 12 is run through and extends to the inside below of culture tank 5 in the bottom of sampling tube 44, be located fixedly connected with drain pipe 45 on the sampling tube 44 of culture tank 5 top, drain pipe 45 downward sloping sets up and is linked together with sampling tube 44, be equipped with stop valve 46 on the drain pipe 45. The sampling can be carried out without opening the top cover 12, the sampling is convenient, and the probability of stem cell pollution is reduced.

Preferably, as shown in FIG. 2, the lower part of the solution supplementing pipe 14 is attached to the side wall inside the culture tank 5, the cross section of the bottom of the solution supplementing pipe 14 is an inclined plane, and the lowest end of the bottom of the solution supplementing pipe 14 is attached to the side wall inside the culture tank 5. The cell culture solution can flow into the culture tank 5 slowly along the side wall of the culture tank 5, and the impact on the stem cells is reduced.

The working principle of the utility model is as follows: during the use, open the valve that sets up both ends about heat source pipe 24, let in homothermal clean water in the heat source pipe 24, keep the constancy of the inside temperature of culture tank 5, inside carbon dioxide and the mixture of air through sterilization treatment entered culture tank 5 through air intake manifold 33, the waste gas that produces in cell growth, the metabolic process got into the stock solution bottle 27 through exhaust hose 35, L type pipe 36 in proper order, for providing continuous fresh clean air in the culture tank 5, provide a good growing environment for the stem cell. In the process of stem cell culture, along with the descending of the liquid level in the culture tank 5, the position of the floating ball component descends under the action of gravity, the floating ball component drives the slide rod 17 and the pressure plate 20 to move downwards in sequence, when the bottom of the pressure plate 20 touches the contact switch 21, the contact switch 21 transmits a signal to the controller 11, the controller controls the electromagnetic valve 15 to open, the cell culture solution in the liquid storage tank 3 enters the culture tank 5 through the liquid replenishing pipe 14, the cell culture solution in the culture tank 5 is supplemented in time, the floating ball component also rises along with the rise of the water level along with the rise of the liquid level in the culture tank 5, the floating ball component drives the slide bar 17 and the press plate 20 to move upwards in sequence, when the bottom of the pressure plate 20 is separated from the contact switch 21, the contact switch 21 transmits a signal to the controller 11, which controls the electromagnetic valve 15 to close, and stops the supply of the cell culture solution into the culture tank 5. In the process of stem cell culture, the carbon dioxide concentration sensor 37 monitors the concentration of carbon dioxide in the culture tank 5 in real time, when the concentration is higher than 5% of the set concentration, the carbon dioxide concentration sensor 37 transmits a signal to the controller 11, the controller 11 regulates and controls the first electromagnetic flow regulating valve 30 and the second electromagnetic flow regulating valve 31, the flow of the carbon dioxide conveyed to the interior of the culture tank 5 is reduced, and the flow of the air is increased, when the concentration is lower than 5% of the set concentration, the carbon dioxide concentration sensor 37 transmits a signal to the controller 11, and the controller 11 regulates and controls the first electromagnetic flow regulating valve 30 and the second electromagnetic flow regulating valve 31, so that the flow of the carbon dioxide conveyed to the interior of the culture tank 5 is increased, and the flow of the air is reduced; when the concentration of the carbon dioxide is restored to 5%, the controller 11 regulates the first electromagnetic flow control valve 30 and the second electromagnetic flow control valve 31 again, so that both are restored to the initial setting state. The degree of automation is high, and the gas atmosphere in the culture tank 5 is always maintained at 5% by volume of carbon dioxide and 95% by volume of air. When the sampling observation is needed, the part of the liquid outlet pipe 45 exposed in the air is disinfected, then the stop valve 46 is opened, the corrugated pipe 43 is held by hand and extruded, the cell liquid in the culture tank 5 enters the sampling pipe 44 under the action of pressure difference and is discharged from the liquid outlet pipe 45, and the stop valve 46 is closed in time after the sampling is finished.

It should be noted that the electrical components of the present invention are all provided with a power supply, and the control method is the prior art, and is unified here for avoiding the redundancy of description; and the present invention is primarily intended to protect mechanical devices, the control means and circuit connections will not be explained in detail herein.

The above embodiments are illustrative of the present invention, and are not intended to limit the present invention, and any simple modifications of the present invention are within the scope of the present invention.

Claims

1. The utility model provides a stem cell culture device, includes the supporting baseplate of level setting, its characterized in that: the two sides of the supporting bottom plate are fixedly provided with symmetrically arranged vertical plates, a liquid storage tank, a mounting plate and a culture tank are sequentially arranged between the two vertical plates from top to bottom, the liquid storage tank and the culture tank are detachably connected with the vertical plates, two ends of the mounting plate are respectively fixed on the inner sides of the two vertical plates, and the top of the mounting plate is provided with a controller; cultivate the top threaded connection of jar and have the top cap, be fixed with T type supporting shoe on the top cap, the intercommunication has the moisturizing pipe between liquid storage pot and the cultivation jar, the mounting panel is run through in proper order to the bottom of moisturizing pipe, the top cap extends to the inside below of cultivation jar, be equipped with the solenoid valve on the moisturizing pipe, the inside top of cultivation jar is provided with the sliding sleeve, the inside sliding connection of sliding sleeve has the slide bar of vertical setting, the top of slide bar extends to the top of top cap and the clamp plate that fixed connection has the level to set up, the clamp plate is located the top of T type supporting shoe, the top of T type supporting shoe is provided with the contact switch, the bottom fixedly connected with who slides the slide bar floats the ball subassembly, the solenoid valve, the contact switch all is connected with the controller electricity.

2. The stem cell culture apparatus of claim 1, wherein: the lateral wall of culture tank is inside hollow bilayer structure, is outer wall and inner wall respectively, and spiral winding has the heat source pipe on the inner wall, and the upper and lower both ends of heat source pipe all run through the outer wall and extend to the outside of culture tank.

3. The stem cell culture apparatus of claim 2, wherein: the top of the mounting plate is fixedly connected with a carbon dioxide gas storage tank, a compressed air gas storage tank and a liquid storage bottle which are arranged at intervals, sterilization water is stored in the liquid storage bottle, the top of the carbon dioxide gas storage tank and the top of the compressed air gas storage tank are respectively communicated with a first gas supply hose and a second gas supply hose, a first electromagnetic flow regulating valve is arranged on the first gas supply hose, a second electromagnetic flow regulating valve is arranged on the second gas supply hose, the first electromagnetic flow regulating valve and the second electromagnetic flow regulating valve are electrically connected with a controller, a three-way connecting pipe is communicated between the first gas supply hose and the second gas supply hose, the other end of the three-way connecting pipe is communicated with a vertically downward air inlet main pipe, a one-way valve is arranged on the air inlet main pipe, the bottom end of the air inlet main pipe sequentially penetrates through the mounting plate and the top cover and extends to the upper part inside the culture tank, and an exhaust hose communicated with the inside of the culture tank is fixed on the top cover, the other end of the exhaust hose penetrates through the mounting plate and is communicated with an L-shaped pipe, the bottom end of the vertical section of the L-shaped pipe is positioned at the lower part of the liquid level of the sterilized water, and a carbon dioxide concentration sensor for feeding back signals to the controller is arranged below the top cover.

4. The stem cell culture apparatus of claim 3, wherein: the bottom fixed connection of air intake house steward has the air inlet dish that the level set up, and the air inlet dish is the circular dish of the hollow setting in inside, and the air vent has been seted up to the intermediate position at air inlet dish top, and air intake house steward's bottom mounting is in the intermediate position at air inlet dish top and cover the air vent, and the inlet port that a plurality of even intervals set up is seted up to the bottom of air inlet dish.

5. The stem cell culture apparatus of claim 3, wherein: the floater assembly comprises a cross-shaped mounting frame, the cross-shaped mounting frame is fixed at the bottom end of the sliding rod, and floating balls are fixedly connected to four end portions of the cross-shaped mounting frame.

6. The stem cell culture apparatus of claim 3, wherein: the top of cultivateing the jar is provided with sampling component, sampling component is including setting up the bellows in the top cap top, and the bellows is inside hollow structure, the sampling tube that the vertical downward setting of bottom fixedly connected with of bellows set up, and the sampling tube is linked together with the bellows, and the bottom of sampling tube runs through the top cap and extends to the inside below of cultivateing the jar, is located fixedly connected with drain pipe on the sampling tube of cultivateing jar top, and the drain pipe downward sloping sets up and is linked together with the sampling tube, is equipped with the stop valve on the drain pipe.

7. The stem cell culture apparatus of claim 3, wherein: the lower part of the liquid supplementing pipe is attached to the side wall inside the culture tank, the cross section of the bottom of the liquid supplementing pipe is an inclined plane, and the lowest end of the bottom of the liquid supplementing pipe is attached to the side wall inside the culture tank.

8. The stem cell culture apparatus of any one of claims 1-7, wherein: the top threaded connection of liquid storage pot has the upper cover, and the intermediate position intercommunication at upper cover top has the vertical charge-in tube that upwards sets up, and the top threaded connection of charge-in tube has the pipe cap.