CN209669255U - Magnetic agitation culture apparatus for zooblast suspension culture - Google Patents
Magnetic agitation culture apparatus for zooblast suspension culture Download PDFInfo
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- CN209669255U CN209669255U CN201920311579.1U CN201920311579U CN209669255U CN 209669255 U CN209669255 U CN 209669255U CN 201920311579 U CN201920311579 U CN 201920311579U CN 209669255 U CN209669255 U CN 209669255U
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Abstract
The utility model belongs to cell culture apparatus technical field, more particularly to a kind of magnetic agitation culture apparatus for zooblast suspension culture, including magnetic stirring apparatus, rotor and culture bottle, culture bottle is placed on the object disposing platform of magnetic stirring apparatus, the structure of culture bottle includes bottle body, cell dispersive vectoring device and bottle cap, bottle body upper end opening, cell dispersive vectoring device can enter inside bottle body from the upper end opening of bottle body, and it is placed in bottom in bottle body, rotor is placed in bottom in bottle body, and it is located inside cell dispersive vectoring device, at cell dispersive vectoring device is located at right above rotor, and place opposite with rotor has the spherical surface protruded to rotor direction, liquid flow port is provided on cell dispersive vectoring device；Bottle cap can be removably mounted at the upper end opening of bottle body.The utility model can be reduced the Cellular Accumulation situation close to rotor top neighbouring position, be conducive to zooblast suspension culture.
Description
Technical field
The utility model belongs to cell culture apparatus technical field, and in particular to it is a kind of for zooblast suspend culture
Magnetic agitation culture apparatus.
Background technique
The zooblast of in vitro culture has wide convenient for observation, operation and detection in the basic research of field of biology
General application.Cell suspension cultures are a kind of training methods of non-anchorage-dependent cell, are made by concussion or rotating device
Culture bottle inner cell is in suspended dispersion.The culture zooblast by way of cell suspension cultures, especially lactation are dynamic
Object cell is easy to implement the probing into of drug mechanism, macro-molecular protein class drug and production of vaccine etc..The prior art
In, the mode being used in combination using magnetic stirring apparatus and culture bottle is cultivated in zooblast suspension, is placed with magnetic in culture bottle
Power rotor can be such that liquid in culture bottle rotates under the action of magnetic stirring apparatus, cell is made to suspend.Since zooblast lacks carefully
Cell wall, so general require to slowly run, the existing matching used rotor of some magnetic stirring apparatus is located at culture bottle bottom in addition,
In rotor rotation process, position is easy accumulation cell between near top 0-15mm, is unfavorable for zooblast suspension culture.Cause
This, needs to develop a kind of magnetic agitation culture apparatus that can be reduced the Cellular Accumulation close to rotor top neighbouring position.
Utility model content
The purpose of the utility model is to provide a kind of magnetic agitation culture apparatus for zooblast suspension culture, can subtract
Few Cellular Accumulation situation close to the position 0-15mm near rotor top is conducive to zooblast suspension culture.
A kind of magnetic agitation culture apparatus for zooblast suspension culture provided by the utility model, including magnetic force stir
Device, rotor and culture bottle are mixed, the culture bottle is placed on the object disposing platform of the magnetic stirring apparatus, and the rotor is placed in training
Support bottom in bottle, which is characterized in that the structure of the culture bottle includes bottle body, cell dispersive vectoring device and bottle cap, the bottle body
Upper end opening, the cell dispersive vectoring device can enter in the bottle body from the upper end opening of the bottle body, and be placed in described
Bottom in bottle body, the rotor is placed in bottom in bottle body, and is located inside the cell dispersive vectoring device, and the cell dispersion is drawn
Device is led at the rotor surface and place opposite with the rotor has the spherical surface protruded to the rotor direction, it is described
Spherical surface bottom and described the distance between rotor top of stating are 2-15mm, are provided with liquid communication on the cell dispersive vectoring device
Mouthful；The bottle cap is removably mounted at the upper end opening of the bottle body.
Preferably, in the magnetic agitation culture apparatus of the above-mentioned culture that suspends for zooblast, the bottle body is upper end
The cylinder of opening.
Preferably, in the magnetic agitation culture apparatus of the above-mentioned culture that suspends for zooblast, the spherical surface bottom with
Described the distance between rotor top of stating is 2-5mm.
Preferably, in the magnetic agitation culture apparatus of the above-mentioned culture that suspends for zooblast, the cell dispersion is drawn
Leading device includes base ring, load-carrying ring, dispersion block, several connecting rods and several bearing bars, and the base ring is placed in the bottle body
Interior bottom, the rotor are located at the base ring centre, and the load-carrying ring is located at right above the base ring, the bearing bar
One end is fixed in the base ring, and the other end is fixed on the load-carrying ring, and the bottom surface of the dispersion block is spherical surface, and described point
It dissipates block and is located at the load-carrying ring centre, and the company of being fixed by the connecting rod between the dispersion block and the load-carrying ring inner sidewall
It connects.
Preferably, in the magnetic agitation culture apparatus of the above-mentioned culture that suspends for zooblast, the cell dispersion is drawn
Leading device includes base ring, dispersion block and at least two load-bearing connecting rods, and the base ring is placed in bottom in the bottle body, described
Rotor is located at the base ring centre, and the bottom surface of the dispersion block is spherical surface, and the dispersion block is located in the base ring
Right above heart point, it is fixedly connected between the dispersion block and the base ring by load-bearing connecting rod.
Preferably, in the magnetic agitation culture apparatus of the above-mentioned culture that suspends for zooblast, the load-bearing connecting rod
Vertical end for L shape bar, and the L shape bar is fixed in the base ring, and horizontal ends are fixed on the dispersion block side wall.
Preferably, in the magnetic agitation culture apparatus of the above-mentioned culture that suspends for zooblast, the cell dispersion is drawn
Leading device includes load-carrying ring, dispersion block, several connecting rods and several bearing bars, and the bearing bar bottom can be placed in the bottle body
Bottom, top are fixed on load-carrying ring, and the bottom surface of the dispersion block is spherical surface, and the dispersion block is located at the load-carrying ring center
Place, and connection is fixed by the connecting rod between the dispersion block and the load-carrying ring inner sidewall.
Preferably, in the magnetic agitation culture apparatus of the above-mentioned culture that suspends for zooblast, the dispersion block is olive
Olive shape or hemispherical, and hemispheric spherical surface, towards bottle body bottom surface direction protrusion, olivary axis is parallel with bottle body bottom surface.
Compared with prior art, a kind of magnetic agitation culture dress cultivated that suspends for zooblast of the utility model
It sets, has the advantages that
1, the utility model is used using magnetic stirring apparatus, rotor engagement, makes zooblast and culture solution stirring in bottle body
Get up, along the spherical surface curved bottom portion of dispersion block upwards or to upper side extension, rotor revolves the liquid perturbation that rotor rotation generates
During turning, the cell of top 0-15mm neighbouring position is crossed also along spherical surface bottom with liquid perturbation upwards or to upper side
Extend, can be reduced the Cellular Accumulation situation close to rotor top neighbouring position, is conducive to zooblast suspension culture.
2, the cell dispersive vectoring device of the utility model can take out from bottle body, which does not have bottle body lid
Have any requirement, do not need to be connected on bottle cap, bottle body can also less open one for cell dispersive vectoring device installation bottle cap with
And corresponding bottle cap opening.
Detailed description of the invention
Fig. 1 is magnetic agitation culture apparatus structural schematic diagram of the utility model for zooblast suspension culture；
Fig. 2 is the first structural schematic diagram of the cell dispersive vectoring device of the utility model；
Fig. 3 is second of structural schematic diagram of the cell dispersive vectoring device of the utility model；
Fig. 4 is the third structural schematic diagram of the cell dispersive vectoring device of the utility model；
Fig. 5 is the structural schematic diagram of the bottle cap of the utility model.
Specific embodiment
With reference to the accompanying drawing, specific embodiment of the present utility model is described in detail, it is to be understood that this is practical
Novel protection scope is not limited by the specific implementation.
A kind of magnetic agitation culture apparatus for zooblast suspension culture, specifically as shown in Figs. 1-5, including magnetic force stirs
Device 1, rotor 2 and culture bottle are mixed, culture bottle is placed on the object disposing platform of magnetic stirring apparatus 1, and rotor 2 is placed in bottom in culture bottle
Portion, magnetic stirring apparatus 1 can directly be bought, for example Beijing is stirred with 5 cell culture magnetic force of SM-5 of moral foundation Science and Technology Ltd.
Device is mixed, the applicable stirring rotator length 20-60mm of the blender；Rotor 2 can also directly be bought, such as on the market common poly- four
Fluorine stirring rotator.
The structure of culture bottle includes bottle body 3, cell dispersive vectoring device 4 and bottle cap, and bottle body 3 is the cylinder of upper end opening,
Bottle body 3 is prepared using glass material, such as the pyrex of low dissolution, and zooblast and cell culture fluid are placed in bottle body 3,
To carry out animal suspension culture.Cell dispersive vectoring device 4 can enter in bottle body 3 from the upper end opening of bottle body 3, and be placed in bottle body
Bottom in 3, rotor 2 is placed in bottom in bottle body 3, and is located inside cell dispersive vectoring device 4, and cell dispersive vectoring device 4, which is located at, to be turned
Opposite place has the spherical surface protruded to 2 direction of rotor, spherical diameter 2-5cm, due to experiment at right above son 2 and with rotor 2
Magnetic agitation root diameter 5-7mm is often used in room, guarantees spherical surface bottom and states the distance between 2 top of rotor in 2-15mm, preferably
Between 2-5mm, distance it is closer, the space between rotor 2 and spherical surface is with regard to smaller, and the cell that can be accumulated is also fewer, rotor 2
It rotates, will not arbitrarily move, rotational stability is good, will not directly touch Spherical Bottom all around itself midpoint in rotation
Portion；Liquid flow port is provided on cell dispersive vectoring device 4.
Specifically, cell dispersive vectoring device 4 has following several structures:
The first: is as shown in Fig. 2, cell dispersive vectoring device 4 includes base ring 41, bearing bar 42, load-carrying ring 43, connecting rod
44 and dispersion block 45, base ring 41 is placed in bottom in bottle body 3, and 42 bottom fixed bonding of bearing bar or integrated molding are connected to
In base ring 41,42 top fixed bonding of bearing bar or integrated molding are connected on load-carrying ring 43, and the quantity of bearing bar 42 is
2-4, and circumferentially shape is uniformly distributed around base ring 41, rotor 2 is located at 41 centre of base ring, and load-carrying ring 43 is located at pedestal
Right above ring 41, and the diameter of load-carrying ring 43 is equal to the diameter of base ring 41, or is slightly less than 41 diameter 2-10mm of base ring, bottom
The diameter of seat ring 41 is greater than used 2 length of rotor, and dispersion block 45 is olive shape or hemispherical, and hemispheric spherical surface court
3 bottom surface direction of bottle body protrusion, olive shape is then that axis is parallel with 3 bottom surface of bottle body, the spherical surface bottom of dispersion block 45 to state rotor 2
The distance between top is in 2-5mm；Dispersion block 45 is located at 43 center of inside of load-carrying ring, and the diameter of dispersion block 45 compares load-carrying ring
43 diameter small 1-3cm is fixedly connected between dispersion block 45 and 43 inner sidewall of load-carrying ring by connecting rod 44, the one of connecting rod 44
End is fixed on 43 inner sidewall of load-carrying ring, and the other end is fixed on 45 lateral wall of dispersion block.
Second, as shown in figure 3, cell dispersive vectoring device 4 includes base ring 41, load-bearing connecting rod 46 and dispersion block 45,
Base ring 41 is placed in bottom in bottle body 3, and the diameter of base ring 41 is greater than the length of used rotor 2, and dispersion block 45 is located at
Right above 41 central point of base ring, rotor 2 is located at 41 centre of base ring, and load-bearing connecting rod 46 is L shape bar, and L shape bar is perpendicular
Straight end fixed bonding or integrated molding are connected in base ring 41, and the horizontal ends of L shape bar are fixedly bonded to 45 side wall of dispersion block
On, the quantity of load-bearing connecting rod 46 is 2-4, and circumferentially shape is uniformly distributed around base ring 41.The structure of dispersion block 45 with
45 structure of dispersion block of the first cell dispersive vectoring device 4 is identical；It the spherical surface bottom of dispersion block 45 and states between the top of rotor 2
Distance is in 2-5mm.
The third, as shown in figure 4, essentially identical with the structure of the first cell dispersive vectoring device 4, difference is: not having
Base ring 41,42 bottom of bearing bar are contacted with bottle body 3, can be placed in bottom in bottle body 3, are fixed on load-carrying ring at the top of bearing bar 42
On 43, the quantity of bearing bar 42 is 3-5, and circumferentially shape is uniformly distributed around load-carrying ring 43.
It should be noted that " liquid flow port " is exactly dispersion block 45 in the structure of above-mentioned three kinds of cell dispersive vectorings device 4
With the gap of load-carrying ring 43 or other component；In order to keep the stability of liquid in bottle body 3 of cell dispersive vectoring device 4 more preferable,
The 45 top fixed bonding of dispersion block of cell dispersive vectoring device 4 or integrated molding are connected with clump weight 8.
It is detachably connected at bottle cap and the upper end opening of bottle body 3；Bottle cap uses the plastic lid of lower ending opening；Alternatively, bottle cap
Using down big up small structure, such as similar to nipple form, funnel shaped, as shown in figure 5, bottle cap includes that upper and lower side is open
Cylindrical body 5 and taper connection body 6, the bottom of cylindrical body 5 can be detachably connected at the upper end opening of bottle body 3；Around bottle body 3
Upper end opening lateral wall offers external screw thread, and the inner wall of cylindrical body 5 offers the internal screw thread for matching and being spirally connected with external screw thread；Cylindrical body
5 top is connected to the lower ending opening of taper connection body 6, and cylindrical body 5 and taper connection body 6 are integrally formed, taper connection body 6
The integrally formed connector 7 for being connected with cylindrical barrel shape of upper end opening, connector 7 for install closing cap or air filter or
Person, which fills in sterile cotton etc., can cross the component of air filtering.
Preferably, inner wall of the junction of cylindrical body 5 and taper connection body 6 apart from cylindrical body 5 has the distance of 0.5-1cm,
Forming annulus between 5 outer wall of cylindrical body, 5 inside of cylindrical body is equipped with gasket 51 at annulus, and gasket 51
Right above 3 top of bottle body, makes to be connected on gasket 51 at the top of bottle body 3, reach sealing effect.
Bottle cap can close bottle body 3, carry out zooblast suspension culture in the environment of a relative closure.
The utility model is used cooperatively using magnetic stirring apparatus 1, rotor 2, makes zooblast and culture solution stirring in bottle body 3
Get up；It is located at right above rotor 2 due to cell dispersive vectoring device 4 and opposite place has to 2 direction of rotor protrusion with rotor 2
Spherical surface, before stirring, then " spherical surface ", that is, the spherical surface of dispersion block 45 are then turned on magnetic positioned at the surface of rotor 2
Power blender 1.It is 2-15mm or so, the liquid that the rotation of rotor 2 generates since distance is small between the spherical surface and rotor 2 of dispersion block 45
Body disturbance is upward along the spherical surface curved bottom portion of dispersion block 45 or extends to upper side, in rotor rotation process, near top
The cell of position is crossed with liquid perturbation also along 45 spherical surface bottom of dispersion block upwards or to upper side extension, finally via liquid
Communication port is suspended in liquid, and " liquid flow port " is exactly the gap of dispersion block 45 and load-carrying ring 43 or other component, hemisphere
Shape or 45 top of olivary dispersion block are also not easy to accumulate cell.Rotor 2 is rotated in rotation all around itself midpoint,
It will not arbitrarily move, rotational stability is good, will not directly touch spherical surface bottom, will not encounter cell dispersive vectoring device 4.It is comprehensive
On, the utility model can be reduced the cell close to 2 position near top 0-15mm of rotor, especially 2 top center position of rotor
Accumulation is conducive to zooblast suspension culture.
The cell dispersive vectoring device 4 of the utility model can take out from bottle body 3, as long as 3 upper end opening size of bottle body is greater than carefully
4 size of born of the same parents' dispersive vectoring device, enables cell dispersive vectoring device 4 to free in and out in bottle body 3；4 pairs of bottles of cell dispersive vectoring device
3 lid of body has no requirement, and does not need to be connected on bottle cap, and bottle body 3 can also open one less pacifies for cell dispersive vectoring device 4
The bottle cap of dress and corresponding bottle cap opening.
It should be noted that " upper and lower " orientation of the invention, refers to corresponding to bottle body 3 in actual use
" upper and lower " orientation, that is, " upper and lower " orientation shown in Fig. 1.
It should be noted that in order to reduce damage of the case to zooblast, in bottle body 3, cell dispersive vectoring device 4
All parts surfaces for including all are smooth surface.Cell dispersive vectoring device 4 is using glass, ceramics or inert metal system
Standby, quality can stablize placement or presence at least more than 5 times of quality of rotor 2 itself in a liquid.Similar in large beaker
A small beaker is placed in back-off, and the rotation of rotor 2 will not influence the positional stability of the small beaker.
Obviously, it is practical without departing from this can to carry out various modification and variations to the utility model by those skilled in the art
Novel spirit and scope.If in this way, these modifications and variations of the present invention belong to the utility model claims and
Within the scope of its equivalent technologies, then the utility model is also intended to include these modifications and variations.
Claims (8)
1. it is a kind of for zooblast suspend culture magnetic agitation culture apparatus, including magnetic stirring apparatus (1), rotor (2) and
Culture bottle, the culture bottle are placed on the object disposing platform of the magnetic stirring apparatus (1), and the rotor (2) is placed in culture bottle
Interior bottom, which is characterized in that the structure of the culture bottle includes bottle body (3), cell dispersive vectoring device (4) and bottle cap, the bottle
Body (3) upper end opening, the cell dispersive vectoring device (4) can enter the bottle body (3) from the upper end opening of the bottle body (3)
It is interior, and it is placed in the bottle body (3) interior bottom, the rotor (2) is placed in bottle body (3) interior bottom, and is located at the cell and disperses
Guiding device (4) is internal, the cell dispersive vectoring device (4) be located at the rotor (2) surface and with the rotor (2) phase
There is the spherical surface protruded to the rotor (2) direction to place, the distance between rotor (2) top is stated with described in the spherical surface bottom
For 2-15mm, liquid flow port is provided on the cell dispersive vectoring device (4)；The bottle cap is removably mounted at the bottle body
(3) at upper end opening.
2. the magnetic agitation culture apparatus according to claim 1 for zooblast suspension culture, which is characterized in that institute
State the cylinder that bottle body (3) are upper end opening.
3. the magnetic agitation culture apparatus according to claim 1 for zooblast suspension culture, which is characterized in that institute
Spherical surface bottom and described the distance between rotor (2) top of stating are stated as 2-5mm.
4. the magnetic agitation culture apparatus according to claim 3 for zooblast suspension culture, which is characterized in that institute
Stating cell dispersive vectoring device (4) includes base ring (41), load-carrying ring (43), dispersion block (45), several connecting rods (44) and several
Bearing bar (42), the base ring (41) are placed in the bottle body (3) interior bottom, and the rotor (2) is located at the base ring
(41) centre, the load-carrying ring (43) are located at right above the base ring (41), and described bearing bar (42) one end is fixed on institute
It states on base ring (41), the other end is fixed on the load-carrying ring (43), and the bottom surface of the dispersion block (45) is spherical surface, and described point
It dissipates block (45) and is located at the load-carrying ring (43) centre, and lead between the dispersion block (45) and the load-carrying ring (43) inner sidewall
Connecting rod (44) is crossed to be fixedly connected.
5. the magnetic agitation culture apparatus according to claim 3 for zooblast suspension culture, which is characterized in that institute
Stating cell dispersive vectoring device (4) includes base ring (41), dispersion block (45) and at least two load-bearing connecting rods (46), the pedestal
Ring (41) is placed in the bottle body (3) interior bottom, and the rotor (2) is located at the base ring (41) centre, the dispersion block
(45) bottom surface is spherical surface, and the dispersion block (45) is located at right above the base ring (41) central point, the dispersion block
(45) it is fixedly connected between the base ring (41) by load-bearing connecting rod (46).
6. the magnetic agitation culture apparatus according to claim 5 for zooblast suspension culture, which is characterized in that institute
Stating load-bearing connecting rod (46) is L shape bar, and the vertical end of the L shape bar is fixed on the base ring (41), and horizontal ends are fixed
On the dispersion block (45) side wall.
7. the magnetic agitation culture apparatus according to claim 3 for zooblast suspension culture, which is characterized in that institute
Stating cell dispersive vectoring device (4) includes load-carrying ring (43), dispersion block (45), several connecting rods (44) and several bearing bars (42),
Bearing bar (42) bottom can be placed in the bottle body (3) interior bottom, and top is fixed on load-carrying ring (43), the dispersion block
(45) bottom surface is spherical surface, and the dispersion block (45) is located at the load-carrying ring (43) centre, and the dispersion block (45) and institute
It states and is fixedly connected between load-carrying ring (43) inner sidewall by connecting rod (44).
8. special according to the described in any item magnetic agitation culture apparatus for zooblast suspension culture of claim 4-7
Sign is that the dispersion block (45) is olive shape or hemispherical, and hemispheric spherical surface is convex towards the bottle body (3) bottom surface direction
It rises, olivary axis is parallel with the bottle body (3) bottom surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920311579.1U CN209669255U (en) | 2019-03-12 | 2019-03-12 | Magnetic agitation culture apparatus for zooblast suspension culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920311579.1U CN209669255U (en) | 2019-03-12 | 2019-03-12 | Magnetic agitation culture apparatus for zooblast suspension culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209669255U true CN209669255U (en) | 2019-11-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920311579.1U Expired - Fee Related CN209669255U (en) | 2019-03-12 | 2019-03-12 | Magnetic agitation culture apparatus for zooblast suspension culture |
Country Status (1)
| Country | Link |
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| CN (1) | CN209669255U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113025484A (en) * | 2021-03-01 | 2021-06-25 | 雷小英 | Intelligent interactive culture equipment for algae microorganisms |
-
2019
- 2019-03-12 CN CN201920311579.1U patent/CN209669255U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113025484A (en) * | 2021-03-01 | 2021-06-25 | 雷小英 | Intelligent interactive culture equipment for algae microorganisms |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191122 |