CN113016388A - Method for inducing loquat axillary buds to flower - Google Patents

Method for inducing loquat axillary buds to flower Download PDF

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Publication number
CN113016388A
CN113016388A CN202110287976.1A CN202110287976A CN113016388A CN 113016388 A CN113016388 A CN 113016388A CN 202110287976 A CN202110287976 A CN 202110287976A CN 113016388 A CN113016388 A CN 113016388A
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loquat
axillary buds
flower
aba
axillary
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安海山
张学英
章加应
徐芳杰
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Shanghai Academy of Agricultural Sciences
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Shanghai Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Wood Science & Technology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Ecology (AREA)
  • Botany (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Health & Medical Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a method for inducing axillary buds of loquat to flower, which prolongs the flowering period of the loquat, solves the problems of concentrated flowering period, short fresh fruit supply period and the like of the loquat, and in production practice, the loquat mainly takes apical bud flowering, the flowering period is from 10 months to 1 month next year each year, the fruit maturation period is concentrated from the middle and last ten days of 5 months to 6 months, and the fruit supply period is short.

Description

Method for inducing loquat axillary buds to flower
Technical Field
The invention relates to the technical field of fruit tree cultivation, in particular to a method for inducing axillary buds of loquats to flower.
Background
The loquat is a rosaceous loquat, evergreen arbor, the loquat is mainly characterized in that the terminal buds grow into flowers, and the loquat has the characteristics of flowering in autumn and winter and fructification in spring and summer, the flower buds are bred in summer with higher temperature, the flowering phase and the young fruit phase are positive and are in the lowest period all year round, flowers and fruits are easily frozen, the loquat yield is seriously influenced, in order to stabilize the loquat yield, a breeder usually avoids the influence of low temperature on the flowers and the young fruits of the loquat by breeding late-flowering loquat varieties or delaying the loquat flowering phase through flowering phase regulation and control, if the flowering time of the loquat can be changed through a certain technical means, the influence of the low temperature on the flowers and the fruits of the loquat is avoided, and the loquat production practice has a great promoting effect.
The spring flower loquat is a new loquat variety bred by the academy of agricultural sciences in Shanghai city, has large fruit type and good quality, has the characteristics of top bud flowering and axillary bud flowering simultaneously, and in Shanghai area, the spring flower loquat is mature in the top bud differentiation period of 7-9 middle ten days and the 9-10 month early blooming, the key period of the transition of the axillary bud to the axillary bud is from the next 2 middle ten days to 4 middle ten days, the axillary bud blooms in the 4 th bottom and the 6 th middle ten days, but under natural conditions, the axillary bud flowering proportion of the spring flower loquat is extremely low, the yield is difficult to form, and the method for improving the axillary bud flowering proportion of the spring flower loquat by adopting certain technical measures has important significance on improving the yield of the Shanghai area loquat is provided by the invention, the method for treating the differentiated loquat bud of the spring flower by using the ABA solution, inducing the axillary bud to complete the flower bud and fruiting, and the method for prolonging the flowering period of the loquat is provided, so that the axillary bud can prolong the flowering period, Improve loquat output and provide technical support.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, in the Shanghai area, the differentiation period of the top flower bud of the spring flower loquat is 7-9 in the middle ten days of the month, the early blooming in the 9-10 months, the key period of the transition of the axillary bud to the axillary bud is 2-4 in the middle ten days of the next year, the axillary bud blooms at the bottom of 4 months, and ripens in 6 in the middle ten days of the month, but in the natural condition, the ratio of the axillary bud to the flower of the spring flower loquat is extremely low, the yield is difficult to form, and the improvement of the ratio of the axillary bud to the flower of the spring flower loquat by certain technical measures has important significance for improving the yield of the loquat in the Shanghai area.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for inducing axillary buds of loquat to flower is carried out according to the following steps:
the method comprises the following steps: axillary bud pretreatment: removing inflorescences or young fruits at the top ends of the branches and tips of the spring flowers and the loquats, and reserving the first axillary buds at the base parts of inflorescences and axils;
step two: preparing the treating fluid, weighing 0.2-0.5 g of abscisic acid (ABA), and using
Fully dissolving 600mL of 75% alcohol, and then fixing the volume to 1000mL by using deionized water to obtain 100-500 ppm of ABA working solution for later use;
step three: treatment of axillary buds: dipping the ABA working solution prepared in the second step by absorbent cotton, uniformly coating the ABA working solution on the axillary buds of the loquat 'spring flowers' treated in the first step, covering the axillary buds of the loquat with a preservative film with the light shading rate of 30-50%, and coating the axillary buds once every 3 days for 4 times;
step four: and after the axillary buds bloom, carrying out flowering phase management according to conventional flower and fruit management measures in loquat production.
Preferably, the loquat is picked from the inflorescence or young fruit at the top of the branch tip of the spring flower loquat, which has the same tree vigor, growth state and growth period.
Preferably, 0.2g, 0.3g, 0.4g and 0.5g of abscisic acid (ABA) are accurately weighed respectively for preparing the solution, fully dissolved by 600mL of 75% alcohol, and then fixed to 1000mL by deionized water to obtain ABA working solutions with final concentrations of 100ppm, 150ppm, 200ppm and 300ppm for later use.
Compared with the prior art, the invention provides a method for inducing loquat axillary buds to flower, which has the following beneficial effects:
the method utilizes exogenous abscisic acid (ABA) solution with certain concentration to smear the axillary buds of the loquat, and is matched with certain flowering phase regulation measures to induce the axillary buds of the loquat to complete flower bud differentiation, blossom and bear fruits, so that the fresh loquat fruit supply period is prolonged.
Drawings
FIG. 1 is a diagram showing the formation of axillary buds of Eriobotrya japonica according to the method for inducing the formation of flowers;
FIG. 2 is a statistical chart of the flower forming proportion of axillary buds of loquat according to the method for inducing the axillary buds of loquat to flower.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1:
referring to fig. 1-2, a method for inducing axillary buds of loquat to flower comprises the following steps:
1) pretreating axillary buds of loquat: picking off inflorescences or young fruits at the top ends of the tips of the branches of the spring flower loquat with consistent tree vigor, growth state and growth period, and reserving the first axillary bud at the base of the inflorescence shaft as shown in figures 1a and b;
2) preparation of ABA treatment: respectively and accurately weighing 0.2g of abscisic acid (ABA), fully dissolving with 600mL of 75% alcohol, and then diluting with deionized water to a constant volume of 1000mL to obtain 100ppm ABA working solution for later use;
3) treating axillary buds of loquat: dipping a series of ABA working solutions with concentrations prepared in the second step into absorbent cotton, uniformly coating the absorbent cotton on the axillary buds of the spring flower loquat processed in the first step, and marking the processed axillary buds; covering with preservative film with shading rate of 40%, smearing once every 3 days for 4 times, and treating axillary buds of each loquat uniformly;
4) and after axillary buds bloom, performing flowering period management according to conventional flower and fruit management measures in loquat production, and performing data statistics and photographing record on flowering time and quantity of the axillary buds of the loquat under each treatment.
Example 1 after applying 100ppm of the mABA working solution, the ratio of axillary buds to flower was 44.33%.
Example 2:
referring to fig. 1-2, a method for inducing axillary buds of loquat to flower is performed according to the following steps,
1) pretreating axillary buds of loquat: picking off inflorescences or young fruits at the top ends of the tips of the branches of the spring flower loquat with consistent tree vigor, growth state and growth period, and reserving the first axillary bud at the base of the inflorescence shaft as shown in figures 1a and b;
2) preparation of ABA treatment: respectively and accurately weighing 0.3g of abscisic acid (ABA), fully dissolving with 600mL of 75% alcohol, and then diluting with deionized water to a constant volume of 1000mL to obtain an ABA working solution with a final concentration of 150ppm for later use;
3) treating axillary buds of loquat: dipping a series of ABA working solutions with concentrations prepared in the second step into absorbent cotton, uniformly coating the absorbent cotton on the axillary buds of the spring flower loquat processed in the first step, and marking the processed axillary buds; covering with preservative film with shading rate of 40%, smearing once every 3 days for 4 times, and treating axillary buds of each loquat uniformly;
4) and after axillary buds bloom, performing flowering period management according to conventional flower and fruit management measures in loquat production, and performing data statistics and photographing record on flowering time and quantity of the axillary buds of the loquat under each treatment.
Example 2 after 150ppm of maba working solution was applied, the axillary bud flowering rate was 55.67%.
Example 3:
referring to fig. 1-2, a method for inducing axillary buds of loquat to flower is performed according to the following steps,
1) pretreating axillary buds of loquat: picking off inflorescences or young fruits at the top ends of the tips of the branches of the spring flower loquat with consistent tree vigor, growth state and growth period, and reserving the first axillary bud at the base of the inflorescence shaft as shown in figures 1a and b;
2) preparation of ABA treatment: respectively and accurately weighing 0.4g of abscisic acid (ABA), fully dissolving with 600mL of 75% alcohol, and then diluting with deionized water to a constant volume of 1000mL to obtain an ABA working solution with a final concentration of 200ppm for later use;
3) treating axillary buds of loquat: dipping a series of ABA working solutions with concentrations prepared in the second step into absorbent cotton, uniformly coating the absorbent cotton on the axillary buds of the spring flower loquat processed in the first step, and marking the processed axillary buds; covering with preservative film with shading rate of 40%, smearing once every 3 days for 4 times, and treating axillary buds of each loquat uniformly;
4) and after axillary buds bloom, performing flowering period management according to conventional flower and fruit management measures in loquat production, and performing data statistics and photographing record on flowering time and quantity of the axillary buds of the loquat under each treatment.
Example 3 after applying 200ppm of the mABA working solution, the axillary bud-to-flower ratio was 44.33%.
Example 4:
referring to fig. 1-2, a method for inducing axillary buds of loquat to flower is performed according to the following steps,
1) pretreating axillary buds of loquat: picking off inflorescences or young fruits at the top ends of the tips of the branches of the spring flower loquat with consistent tree vigor, growth state and growth period, and reserving the first axillary bud at the base of the inflorescence shaft as shown in figures 1a and b;
2) preparation of ABA treatment: respectively and accurately weighing 0.5g of abscisic acid (ABA), fully dissolving with 600mL of 75% alcohol, and then diluting with deionized water to a constant volume of 1000mL to obtain an ABA working solution with a final concentration of 300ppm for later use;
3) treating axillary buds of loquat: dipping a series of ABA working solutions with concentrations prepared in the second step into absorbent cotton, uniformly coating the absorbent cotton on the axillary buds of the spring flower loquat processed in the first step, and marking the processed axillary buds; covering with preservative film with shading rate of 40%, smearing once every 3 days for 4 times, and treating axillary buds of each loquat uniformly;
4) and after axillary buds bloom, performing flowering period management according to conventional flower and fruit management measures in loquat production, and performing data statistics and photographing record on flowering time and quantity of the axillary buds of the loquat under each treatment.
Example 4 after applying 300ppm of the mABA working solution, the axillary bud flowering rate was 66.67%.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (3)

1. A method for inducing axillary buds of loquat to flower is carried out according to the following steps:
the method comprises the following steps: axillary bud pretreatment: removing inflorescences or young fruits at the top ends of the branches and tips of the spring flowers and the loquats, and reserving the first axillary buds at the base parts of inflorescences and axils;
step two: preparing a treatment solution, namely weighing 0.2-0.5 g of abscisic acid (ABA), fully dissolving with 600mL of 75% alcohol, and then diluting with deionized water to a constant volume of 1000mL to obtain an ABA working solution with a final concentration of 100-500 ppm for later use;
step three: treatment of axillary buds: dipping the ABA working solution prepared in the second step by absorbent cotton, uniformly coating the ABA working solution on the axillary buds of the loquat 'spring flowers' treated in the first step, covering the axillary buds of the loquat with a preservative film with the light shading rate of 30-50%, and coating the axillary buds once every 3 days for 4 times;
step four: and after the axillary buds bloom, carrying out flowering phase management according to conventional flower and fruit management measures in loquat production.
2. The method for inducing axillary buds of loquat to bloom as claimed in claim 1, wherein the loquat is selected from inflorescences or young fruits at the top of the tips of the branches of the 'spring flower' loquat whose vigor, growth state and growth period are the same, and then removed.
3. The method for inducing axillary buds of loquat to flower according to claim 1, wherein the solution is prepared by accurately weighing 0.2g, 0.3g, 0.4g and 0.5g abscisic acid (ABA), dissolving with 600mL of 75% ethanol, and diluting to 1000mL with deionized water to obtain final concentrations of 100ppm, 150ppm, 200ppm and 300ppm ABA working solution for further use.
CN202110287976.1A 2021-03-17 2021-03-17 Method for inducing loquat axillary buds to flower Pending CN113016388A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103518581A (en) * 2013-10-31 2014-01-22 湛江师范学院 Simple method for promoting AP sweetsop flower formation
CN106982661A (en) * 2017-04-14 2017-07-28 上海市农业科学院 A kind of flower and fruit thinning method of citrus
CN108713434A (en) * 2018-04-28 2018-10-30 会东县隆峰农业农机服务有限公司 A kind of processing method of loquat volume increase
CN109197254A (en) * 2018-09-30 2019-01-15 云南盛节农牧开发有限公司 A method of the regulation meadow sweet florescence
CN109673303A (en) * 2019-02-19 2019-04-26 湖南省园艺研究所 A kind of florescence control method postponing cuckoo flowering time

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103518581A (en) * 2013-10-31 2014-01-22 湛江师范学院 Simple method for promoting AP sweetsop flower formation
CN106982661A (en) * 2017-04-14 2017-07-28 上海市农业科学院 A kind of flower and fruit thinning method of citrus
CN108713434A (en) * 2018-04-28 2018-10-30 会东县隆峰农业农机服务有限公司 A kind of processing method of loquat volume increase
CN109197254A (en) * 2018-09-30 2019-01-15 云南盛节农牧开发有限公司 A method of the regulation meadow sweet florescence
CN109673303A (en) * 2019-02-19 2019-04-26 湖南省园艺研究所 A kind of florescence control method postponing cuckoo flowering time

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘宗莉 等: "枇杷花芽和营养芽形成过程中内源激素的变化", 《园艺学报》 *

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Application publication date: 20210625