CN113005073A - Nutrient for promoting microbial growth and preparation method and application thereof - Google Patents

Nutrient for promoting microbial growth and preparation method and application thereof Download PDF

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CN113005073A
CN113005073A CN202110496343.1A CN202110496343A CN113005073A CN 113005073 A CN113005073 A CN 113005073A CN 202110496343 A CN202110496343 A CN 202110496343A CN 113005073 A CN113005073 A CN 113005073A
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perlite
nutrient
vitamin
microbial growth
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CN113005073B (en
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马东兵
阿部哲弥
岳峥
王爱平
潘晓峰
刘学
李军
王广成
韩潇
崔永新
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347—Use of yeasts or fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/06—Contaminated groundwater or leachate
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

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Abstract

The invention relates to a microbial growth promoting nutritional agent, and a preparation method and application thereof. The nutrient is prepared from the following raw materials: perlite, glucose, yeast extract powder and inorganic metal salt, and the preparation method comprises the following steps: adding glucose, yeast extract powder and inorganic metal salt into a reaction container according to a preset proportion, and adding a proper amount of water to completely dissolve the glucose, the yeast extract powder and the inorganic metal salt to obtain a mixed solution; grinding perlite to obtain powder; mixing the powder with the mixture, stirring, drying, and grinding. The invention also discloses the application of the microbial growth promoting nutrient in promoting the sewage treatment bacteria to treat sewage. The nutrient of the invention can make the sewage treatment bacteria become dominant flora in a short time, and provide nutrient components required by normal metabolism of the sewage treatment bacteria for a long time, thereby obviously improving the sewage treatment efficiency.

Description

Nutrient for promoting microbial growth and preparation method and application thereof
Technical Field
The invention belongs to the technical field of microorganism application, and particularly relates to a microbial growth promoting nutrient as well as a preparation method and application thereof.
Background
With the continuous improvement of the socialization degree of human beings and the increasing development of industrial economy, more and more ecological environment problems are increasingly shown, wherein the problem of water body pollution is particularly severe. The discharge of a large amount of industrial wastewater and domestic sewage caused by the acceleration of the urbanization process is a main reason of water body pollution. At present, people continuously try to adopt various physical, chemical or biological approaches to treat and purify sewage so as to improve the water quality of a water body and relieve the continuous and severe current situation of fresh water resource supply.
The microorganism treatment method is a sewage purification mode which is developed rapidly in recent years. The conventional microorganism treatment method is to consume or decompose pollutants in the sewage by using the metabolic activity of microorganisms so as to achieve the final purpose of decomposing or reducing the purified water body. For example, CN208700644U discloses a microbial filtration membrane for sewage treatment, wherein a microbial culture layer is closely attached to a microbial layer, so that microbes on the microbial membrane can absorb and decompose harmful components in sewage, thereby achieving a good filtration effect; CN103570132B discloses that a layer of microbial film is formed on an aquatic plant by placing the aquatic plant in an anaerobic microbial community for culture, and then placing the cultured aquatic plant in a pretreated river water area for water purification; CN108034602B discloses a complex microbial inoculum for heavy metal wastewater treatment, which comprises streptomyces californicus, mucor epidermidis and cellulolytic bacteria; CN103667141B discloses a microbial inoculum for industrial sewage treatment, which contains various bacillus, yeast, nitrobacteria, paracoccus denitrificans and the like, and can efficiently remove suspended matters in sewage.
In order to promote the mass propagation of microorganisms in sewage and further ensure the quantity and activity of microorganisms in sewage, people try to add certain nutrient substances to meet the requirements of the microorganisms except for controlling proper pH value, temperature and other conditions. The conventional method is to add urea, liquid ammonia, ammonium nitrate, ammonium sulfate and the like, but the nutrient substances are single and cannot meet the requirements of microorganisms on various nutrients. Some nutrient raw material components disclosed in the prior art are too complex and have few elements beneficial to microorganisms, and the normal living metabolism of the sewage treatment microorganisms cannot be normally ensured. In addition, in some microbial nutrients, beneficial nutritional ingredients have a short onset time and are difficult to meet the long-term nutritional needs of microorganisms.
Therefore, how to cultivate a large amount of microorganisms for sewage treatment in a short time, provide nutrients required for maintaining normal physiological metabolic activities for a long time and realize large degradation and removal of harmful pollutants in sewage is a problem which needs to be solved for realizing a healthy and sustainable sewage treatment mode.
Disclosure of Invention
The invention aims to provide a nutrient for promoting the growth of microorganisms, which contains macroelements, microelements, vitamins and other nutrients required by the growth and reproduction of the microorganisms, and promotes the rapid growth of the microorganisms, particularly bacillus and the like, into dominant flora; the continuous supply of nutrient elements is realized through the synergistic effect of the mineral carrier containing the pore structure and the nutrient substances, and the purification effect of the sewage is further improved.
The purpose of the invention is realized by the following technical scheme:
according to one aspect of the invention, a microbial growth promoting nutritional agent is provided, which is prepared from the following raw materials: perlite, glucose, yeast extract powder and inorganic metal salt.
Wherein the mass parts of the perlite, the glucose, the yeast extract powder and the inorganic metal salt are respectively 150 parts of perlite, 50-70 parts of glucose, 8-15 parts of yeast extract powder and 5-15 parts of inorganic metal salt.
Preferably, the mass parts of the perlite, the glucose, the yeast extract powder and the inorganic metal salt are respectively 140 parts of perlite 110-.
Wherein the particle size of the perlite is 100-1000 um.
Preferably, the perlite consists of a fraction with a particle size of 100-.
Wherein the mass ratio of the perlite part with the particle size of 100-400 mu m to the perlite part with the particle size of 500-800 mu m is (3-7): 1; preferably, the mass ratio of the perlite part with the particle size of 100-400um to the perlite part with the particle size of 500-800um is (4-6): 1.
the inventor unexpectedly finds that the two kinds of perlite with different particle sizes are used as carriers of nutrient components, the speed of releasing nutrient substances is different, and the inventor finds that the perlite with large particle size and small particle size is compounded in a certain proportion through a large number of experiments to be used as the carrier of the nutrient substances, so that the rapid propagation of microorganisms in a short time can be simultaneously realized to establish dominant floras, and simultaneously, the nutrition required by the dominant floras can be ensured in a long time period. In the present invention, the starting materials may be milled to the desired particle size by any method known in the art, such as ball milling.
Wherein the inorganic metal salt comprises calcium chloride, potassium dihydrogen phosphate, magnesium sulfate, ferric chloride and disodium hydrogen phosphate.
Wherein, the inorganic metal salt comprises 2.5 to 7 portions of calcium chloride, 1.5 to 3 portions of monopotassium phosphate, 0.6 to 3 portions of magnesium sulfate, 0.2 to 1 portion of ferric chloride and 0.2 to 1 portion of disodium hydrogen phosphate according to the weight portion.
Preferably, the inorganic metal salt comprises 3 to 5 parts by weight of calcium chloride, 2 to 2.5 parts by weight of potassium dihydrogen phosphate, 1.5 to 2.5 parts by weight of magnesium sulfate, 0.4 to 0.8 part by weight of ferric chloride and 0.4 to 0.8 part by weight of disodium hydrogen phosphate.
Wherein, the microorganism growth promoting nutrient also comprises 2 to 6 parts of B vitamins. Preferably, the B vitamins are selected from one or more of vitamin B1 (thiamine), vitamin B2 (riboflavin), vitamin B3 (niacin), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine), vitamin B7 (biotin), vitamin B9 (folic acid) and vitamin B12 (cobalamin). Preferably, the B vitamins are a combination of vitamin B3 (niacin) and vitamin B12 (cobalamin).
According to another aspect of the present invention, there is provided a method for preparing a nutrient for promoting microbial growth, comprising: adding glucose, yeast extract powder and inorganic metal salt into a reaction vessel according to a preset proportion, optionally adding B vitamins, and adding a proper amount of water to completely dissolve the B vitamins to obtain a mixed solution; grinding perlite, and sieving to obtain powder; grinding perlite, and sieving to obtain powder; mixing the powder with the mixture, stirring, drying, and grinding.
In the method, perlite, glucose, yeast extract powder and inorganic metal salt are added into a reaction vessel in a mass ratio of (100- > 150): 50-70): 8-15: 5-15.
Optionally, vitamins are added to the reaction vessel along with glucose, yeast extract powder and inorganic metal salts.
According to another aspect of the invention, the application of the microorganism growth promoting nutrient agent in promoting microorganism in sewage treatment is provided, wherein the microorganism is used for purification treatment of municipal sewage, garbage leachate wastewater, kitchen and kitchen garbage wastewater, river black and odorous water body or industrial wastewater and the like.
Relative to the total volume of the inlet water of the biological pond, the nutrient for promoting the growth of the microorganisms is 0.5-2g/m3D, preferably in the range from 1 to 1.5g/m3D, adding.
In the application, the microorganism is a microorganism in a microorganism composite bacteria preparation for sewage treatment.
According to another aspect of the invention, the nutrient provided by the invention is a microbial compound bacteria preparation for sewage treatment, and the active ingredients of the microbial compound bacteria preparation comprise microbial floras of the following genera: the active ingredients of the composition comprise microbial flora of the following genera: bacillus (Bacillus) bacteria, Pseudomonas (Pseudomonas) bacteria, Lactobacillus (Lactobacillus) bacteria, Torulopsis (Torulopsis) fungi, Aspergillus (Aspergillus) fungi, Rhizopus (Rhizopus) fungi, Penicillium (Penicillium) fungi, Mucor (Mucor) fungi, Trichoderma (Trichoderma) fungi, Glucomyces (Thiocapsa) bacteria, Azotobacter (Azotobacter) bacteria, and Nitrosomonas (Nitrosomonas) bacteria.
Wherein, the microbial compound bacteria preparation comprises 65 to 85 portions of bacillus bacteria, 2 to 10 portions of pseudomonas bacteria, 0.1 to 2.5 portions of lactobacillus bacteria, 0.1 to 2.5 portions of Torulopsis fungi, 0.1 to 2.5 portions of Aspergillus fungi, 0.1 to 2.5 portions of Rhizopus fungi, 1 to 5 portions of Penicillium fungi, 0.1 to 2.5 portions of Mucor fungi, 1 to 5 portions of Trichoderma fungi, 0.1 to 2.5 portions of Streptomycete bacteria, 1 to 5 portions of Azotobacter bacteria and 1 to 5 portions of Nitrosomonas bacteria by weight.
Preferably, the microbial compound bacteria preparation comprises 70-80 parts of bacillus bacteria, 3-8 parts of pseudomonas bacteria, 0.5-2 parts of lactobacillus bacteria, 0.5-2 parts of Torulopsis fungi, 0.5-2 parts of Aspergillus fungi, 0.5-2 parts of Rhizopus fungi, 2-4 parts of Penicillium fungi, 0.5-2 parts of Mucor fungi, 2-4 parts of Trichoderma fungi, 0.3-2 parts of Glutinosa bacteria, 2-4 parts of azotobacter bacteria and 2-4 parts of nitrosomonas bacteria by weight.
Wherein, the microbial compound bacteria preparation also comprises the following microbial flora: eremothecium (Eremothecium) fungi, Streptomyces (Streptomyces) bacteria, Neurospora (Neurospora) fungi, and Acetobacter (Acetobacter) bacteria.
Wherein, according to the weight portion, the microorganism compound bacteria preparation also comprises: 0.1-2.5 parts of eremothecium fungus, 0.1-2.5 parts of streptomyces bacteria, 0.1-2.5 parts of neurospora fungus and 0.2-3 parts of acetobacter bacteria.
Preferably, the microbial compound bacteria preparation further comprises the following components in parts by weight: 0.2-2 parts of eremothecium fungi, 0.2-2 parts of streptomyces bacteria, 0.2-2 parts of neurospora fungi and 0.5-2 parts of acetobacter bacteria.
Wherein, in the composite bacteria preparation, the ratio of the fungi to the bacteria in parts by weight is 1: (6-15); preferably, the ratio of the fungi to the bacteria in parts by weight is 1: (7-12).
Wherein, the microbial compound bacteria preparation also comprises a filler.
Wherein the filler is a sustained release granule comprising a sugar material. Wherein the sugar substance is selected from one or more of glucose, fructose, maltose, sucrose, galactose, lactose, mannose and cellobiose.
Wherein, the preparation method of the sustained-release granules containing the sugar substances comprises the following steps: adding sugar substance with final concentration of 50-80g/L, stearic acid with final concentration of 10-30g/L, agar powder with final concentration of 10-20g/L and polyethylene glycol with final concentration of 10-30g/L into deionized water to prepare mixed solution; sterilizing at 100-120 deg.C for 20-40min, dripping the mixed solution into aseptic ice bath dimethyl silicone oil under aseptic condition to obtain granules with particle diameter of about 2-4mm, and absorbing dimethyl silicone oil on the surface of the granules with aseptic paper to obtain the slow release granules containing sugar substances.
Wherein, the raw materials of the microbial compound bacteria preparation also comprise the following components in parts by weight: and 30-50 parts of a filler.
Among them, the Bacillus bacteria include, but are not limited to, Bacillus pumilus (Bacillus pumilus), Bacillus subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformis), Bacillus megaterium (Bacillus megaterium), Bacillus thuringiensis (Bacillus thuringiensis), Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Bacillus cereus (Bacillus cereus), Bacillus polymyxa (Bacillus polymyxa), and Bacillus Natto (Bacillus Natto).
Wherein the Penicillium fungi include, but are not limited to, Penicillium chrysogenum (Penicillium chrysogenum), Penicillium Camemberti (Penicillium Camemberti), and Penicillium lilacinum (Penicillium Luteum).
The technical scheme of the invention has the following advantages or beneficial effects:
(1) according to the growth characteristics of the active dominant bacteria, the nutrient provided by the invention reasonably compounds a carbon source, a nitrogen source, various large or trace elements and vitamins required by the growth of microorganisms, so that sewage treatment bacteria, particularly bacillus, can become dominant flora in a short time, and can provide sufficient nutrients for the growth and metabolic activities of the sewage treatment bacteria in a longer period.
(2) The nutrient provided by the invention takes the perlite which has a microporous structure and is rich in a large amount of active silicon, aluminum, magnesium, calcium and other metal ions as a nutrient carrier, and through the combination of perlite particles with small particle size and large particle size, the double effects of quick release of surface nutrients and slow release of nutrients in micropores are realized, so that sufficient nutrients are provided for microorganisms for a long time to maintain the activity of the microorganisms.
(3) The microbial growth promoting nutrient has the advantages of simple preparation method, wide raw material source and low cost, and can realize mass production.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to specific embodiments. It is understood by those skilled in the art that the embodiments described below are for illustrative purposes only and are not all of the present invention. Based on the embodiments of the present invention, those skilled in the art will better understand and appreciate the technical solutions claimed in the present invention and the technical effects achieved thereby.
The various chemicals in the examples below are commercially available products. Each species in the examples was purchased from a green organism.
The biological rotating disk used in the embodiment of the invention is purchased from Japan Guangen industry Co., Ltd, the material of the biological rotating disk is mainly polyvinylidene chloride, and the main working parameters are as follows: a biological disk-loading device comprises 28 disks with the diameter of 2m and the distance of 50mm, and continuously operates to treat sewage which comes from inlet water of urban sewage plants and has the water treatment amount of 3500m3/d。
Preparation of nutrient for promoting growth of microbe
Example 1
(1) Mixing glucose 60 parts, yeast extract powder 12 parts, calcium chloride 4.5 parts, and potassium dihydrogen phosphate (KH)2PO4)2.3 parts of magnesium sulfate (MgSO)4·7H2O)2 parts, iron chloride (FeCl)3)0.6 part and disodium hydrogen phosphate (Na)2HPO4)0.6 part of the mixture is sequentially added into a reaction container, and a proper amount of water is added and stirred to be completely dissolved, so that a mixed solution is obtained;
(2) grinding 120 parts of perlite, and sieving to obtain perlite powder with the particle size of 250 um;
(3) mixing the perlite powder and the mixed solution, fully stirring, drying at 40 ℃ to constant weight, and grinding into powder to obtain the microbial growth promoting nutritional agent.
Example 2
The preparation of the raw materials and the procedure were the same as in example 1, except that the perlite in step (2) was ground to give a perlite powder having a particle size of 120 um.
Example 3
The preparation of the raw materials and the procedure were the same as in example 1 except that the perlite in step (2) was ground to give a perlite powder having a particle size of 1000 um.
Example 4
The raw materials and procedures were the same as in example 1, except that the perlite processing step in step (2) was replaced with the following steps:
(2) grinding and sieving 100 parts of perlite to obtain powder with the particle size of 250um, grinding and sieving 20 parts of perlite to obtain powder with the particle size of 600um, and mixing to obtain mixed powder.
Example 5
(1) 51 parts of glucose, 15 parts of yeast extract powder, 2.5 parts of calcium chloride and potassium dihydrogen phosphate (KH)2PO4)1.5 parts of magnesium sulfate (MgSO)4·7H2O)1 part, ferric chloride (FeCl)3)0.6 part and disodium hydrogen phosphate (Na)2HPO4)0.6 part of the mixture is sequentially added into a reaction container, and a proper amount of water is added and stirred to be completely dissolved, so that a mixed solution is obtained;
(2) grinding and sieving 100 parts of perlite to obtain powder with the particle size of 250um, grinding and sieving 20 parts of perlite to obtain powder with the particle size of 600um, and mixing to obtain mixed powder;
(3) mixing the perlite powder and the mixed solution, fully stirring, drying at 40 ℃ to constant weight, and grinding into powder to obtain the microbial growth promoting nutritional agent.
Example 6
(1) Mixing glucose 60 parts, yeast extract powder 8 parts, calcium chloride 2.5 parts, and potassium dihydrogen phosphate (KH)2PO4)1.8 parts of magnesium sulfate (MgSO)4·7H20.6 portion of O) and ferric chloride (FeCl)3)0.2 parts of disodium hydrogen phosphate (Na)2HPO4)0.6 part of the mixture is added into a reaction vessel in turn, and a proper amount of water is added and stirred to be completeDissolving to obtain a mixed solution;
(2) grinding and sieving 100 parts of perlite to obtain powder with the particle size of 250um, grinding and sieving 20 parts of perlite to obtain powder with the particle size of 600um, and mixing to obtain mixed powder;
(3) mixing the perlite powder and the mixed solution, fully stirring, drying at 40 ℃ to constant weight, and grinding into powder to obtain the microbial growth promoting nutritional agent.
Example 7
(1) Mixing glucose 52 parts, yeast extract powder 12 parts, calcium chloride 6.5 parts, and potassium dihydrogen phosphate (KH)2PO4)3 parts of magnesium sulfate (MgSO)4·7H2O)3 parts, iron chloride (FeCl)3)1 part and disodium hydrogen phosphate (Na)2HPO4)0.5 part of the mixture is sequentially added into a reaction container, and a proper amount of water is added and stirred to be completely dissolved, so that a mixed solution is obtained;
(2) grinding and sieving 100 parts of perlite to obtain powder with the particle size of 250um, grinding and sieving 20 parts of perlite to obtain powder with the particle size of 600um, and mixing to obtain mixed powder;
(3) mixing the perlite powder and the mixed solution, fully stirring, drying at 40 ℃ to constant weight, and grinding into powder to obtain the microbial growth promoting nutritional agent.
Example 8
Other preparation materials and other steps were the same as in example 4, except that in step (1), 34 parts of vitamin B was added.
Example 9
Other preparation materials and other steps were the same as in example 4, except that in step (1), 32 parts of vitamin B and 122 parts of vitamin B were added.
Comparative example 1
The other preparation of the raw materials and the other steps were the same as in example 1, except that no perlite was added.
Comparative example 2
The raw materials were prepared and the other steps were the same as in example 1 except that the perlite was replaced with bentonite.
(II) preparation of microbial composite bacteria preparation
(1) Preparation of compound bacteria preparation I:
step 1: respectively activating and expanding bacillus megaterium, bacillus natto, Pseudomonas aeruginosa (Pseudomonas aeruginosa), Lactobacillus acidophilus (Lactobacillus acidophilus), Torulopsis histolytica (Torulopsis histolytica), Aspergillus niger (Aspergillus niger), Rhizopus nigricans (Rhizopus nigricans), Penicillium salmonella camembensis (Penicillium camembertii), Penicillium lilacinum (Penicillium Luteum), Mucor racemosus (Mucor racemosus) fungi, Trichoderma viride (Trichoderma viride), thiothrix persicum (Thiocapsa roseopis), Azotobacter chroococcum (Azotobacter chroococcum) and Nitrosomonas bacterium (nitromonas europaea) in a liquid LB culture medium to obtain expanded cultures;
step 2: according to the weight part of the culture, 75 parts of bacillus megatherium, 1 part of bacillus natto, 5 parts of pseudomonas aeruginosa, 1 part of lactobacillus acidophilus, 0.5 part of sulfolobus persicae, 3 parts of azotobacter chroococcum and 2.5 parts of nitrosomonas europaea are inoculated into a liquid culture medium 1 (the formula is as follows: peptone 3g/L, NaCl 5g/L, K)2HPO40.3g/L, glucose 10g/L and water in balance, pH 7.5), and mixed culturing at 28 deg.C until the viable bacteria concentration of each bacterium in the liquid fermentation broth is not less than 1 × 109CFU/ml to obtain mixed culture 1;
and step 3: an expanded culture of 1 part of histolyticum Torulopsis glabrata, 1 part of Aspergillus niger, 1 part of Rhizopus nigricans, 1 part of penicillium salmonellae, 1 part of Penicillium chrysogenum, 1 part of Mucor racemosus and 3 parts of Trichoderma viride is inoculated into a liquid culture medium 2 (the formula is as follows: sucrose 30g/L, NaNO3 2g/L、K2HPO4 1g/L、MgSO4·7H2O 0.5g/L、KCl0.5g/L、FeSO4·7H2O0.2 g/L and water in balance, pH 7.0), mixed and cultured at 28 deg.C until the viable bacteria concentration of each bacterium in the culture solution is not less than 1 × 109CFU/ml to obtain mixed culture 2;
4) and uniformly mixing the mixed culture 1 and the mixed culture 2, and performing centrifugal separation and drying to obtain the microbial compound bacteria preparation I.
(2) Preparing a compound bacterium preparation II:
preparing the microbial compound bacteria preparation according to the same raw materials and steps as the preparation method of the compound bacteria preparation I, wherein 40 parts of sustained-release particles containing glucose are added in the step 4),
wherein the glucose sustained release granules are prepared by: adding 60g/L glucose, 20g/L stearic acid, 15g/L agar powder and 20g/L polyethylene glycol 6000 (the content is more than or equal to 99%) into deionized water to prepare a mixed solution; sterilizing at 110 deg.C for 30min, dripping the mixed solution into aseptic ice bath dimethyl silicone oil under aseptic condition, stirring, mixing to obtain granule with particle diameter of about 2mm, and removing dimethyl silicone oil from the surface of the granule with aseptic paper to obtain glucose-containing sustained release granule.
(3) Preparing a compound bacterium preparation III:
the microbial composite bacterial preparation was prepared according to the same raw materials and procedures as the preparation method of the composite bacterial preparation I, wherein the following fungi and bacteria were further added in the following proportions.
0.5 part of eremothecium ashbyi, 0.5 part of red bread mold, 0.5 part of streptomyces olivaceus and 1.5 parts of acetobacter aceti.
Wherein, the streptomyces olivorubidus and the acetobacter aceti are mixed and cultured in the mixed culture medium 1 in the step 2); eremothecium ashbyii and Nostoc sphaeroides were mixed-cultured in the mixed medium 2 of step 3).
(4) Preparing a compound bacterium preparation IV:
the microbial composite bacterial preparation is prepared according to the same raw materials and steps as the preparation method of the composite bacterial preparation III, wherein 40 parts of the glucose-containing slow-release particles which are the same as those in the composite bacterial preparation II are added in the step 4).
(III) Sewage treatment
The first step is as follows: municipal sewage (raw water: CODcr 347mg/L, BOD)5＝183mg/L，TN＝49mg/L，TP＝4.6mg/L，NH3-N-37 mg/L) sequentially passing through a coarse grid well, a fine grid well and a grit chamber to remove suspended substances and impurities in the wastewater and precipitate precipitates in the water;
the second step is that: enabling the effluent of the grit chamber to flow into an intermediate water tank for mixing and adjusting water quality and water quantity, wherein the effluent of the intermediate water tank automatically flows into a biological rotating disc tank, and the hydraulic retention time is about 30 minutes;
the third step: in order to fully utilize the carbon source in the raw water, the effluent of the biological rotating disk tank flows through a first-stage denitrification tank, a first-stage nitrification tank, a second-stage denitrification tank and a second-stage nitrification tank, wherein the first-stage nitrification tank and the second-stage nitrification tank are provided with microporous aeration disks, the first-stage nitrification tank is provided with an internal reflux pump to enable nitrified liquid to reflux to the first-stage denitrification tank, and the internal reflux quantity is 1Q. Wherein in the primary nitrification tank, the water is fed at 45g/m relative to the total volume of the water fed into the biological tank3The microbial compound bacteria preparation I prepared in the above way is added into the primary nitrification tank, and then 1g/m3D, adding the prepared nutrient for promoting the growth of the microorganisms into the primary nitrification tank for multiple times to ensure the flora dominance of the microorganism composite bacteria;
the fourth step: the effluent of the secondary nitrification tank flows into a secondary sedimentation tank, the secondary sedimentation tank carries out solid-liquid separation on the aerated mixed liquid, the precipitated sludge is pumped to an intermediate water tank by an external reflux pump arranged in an external reflux pump tank, and the reflux quantity is 0.5Q;
the fifth step: and the effluent of the secondary sedimentation tank is discharged after flowing through the disinfection system.
Respectively measuring the BOD of the treated sewage by adopting industrial or national standards5(biological oxygen demand), CODcr(chemical oxygen demand, calculated as Cr), TN, TP and NH3-N (ammonia nitrogen). Specifically, TN is measured by alkaline potassium persulfate digestion ultraviolet spectrophotometry (GB11894-89), TP is measured by ammonium molybdate spectrophotometry (GB 11893-89), and NH is measured3-N was measured by gas phase molecular absorption spectroscopy (HJ/T195-2005), COD was measured by potassium dichromate method (GBT11914-89) and BOD was measured by dilution and inoculation method (HJ 505-2009).
The results of the municipal sewage measurement are shown in table 1 below:
TABLE 1
Figure BDA0003054472650000091
As can be seen from Table 1, the nutrient-treated BOD containing perlite5CODcr, TN, TP and NH3The removal effect of-N is obviously better than that of a nutrient without perlite, which shows that the perlite promotes the purification of sewage by the microorganism composite bacteria to a certain extent. In addition, the sewage purification effect of the nutrient containing perlite of the present invention is significantly better than the treatment of the nutrient containing bentonite (comparative example 2), probably because: when the bentonite is dispersed in sewage, the bentonite can adsorb various pollutants with larger volume in the sewage more quickly than perlite, and meanwhile, the viscosity of the bentonite is increased, so that beneficial flora and the pollutants in the sewage are adsorbed and settled together, the number of microbial active bacteria dispersed in the sewage is greatly reduced, and the sewage purification effect is seriously influenced.
The nutrient treatment containing vitamin B (examples 8 to 9) has the best effect of purifying sewage, wherein N, P and pollutants treated by the nutrient treatment compounded by vitamin B3 and vitamin B12 have the best effect of purifying sewage, and probably because the synergistic interaction of vitamin B3 (nicotinic acid) and vitamin B12 (cobalamin) promotes the rapid growth and proliferation of various bacteria, particularly bacillus, in the complex microbial inoculum, so that the dominant flora can be maintained and active substances such as various enzymes and the like are secreted for a long time, and the removal effect of N, P and pollutants in sewage is greatly improved.
In addition, based on the nutrient of example 9, the sewage purification effect of different complex bacteria preparations is further improved, and the results are shown in the following table 2:
TABLE 2
Figure BDA0003054472650000101
As can be seen from Table 2 above, the sewage-purifying effect of the complex bacterial preparation containing the glucose sustained-release granules is superior to that of the complex bacterial preparation containing no glucose. In addition, the complex microbial inoculum containing the combination of the eremothecium ashbyi, the bread fungus, the streptomyces olivaceus and the acetobacter aceti has the BOD5CODcr, TN, TP and NH3The better removal of-N may be due to the presence of these bacteriaCertain synergistic effect exists, and certain organic acid or active enzyme substances secreted by the bacteria promote the decomposition and removal of sewage pollutants.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Those skilled in the art will appreciate that various substitutions, modifications and combinations of features of the invention may be made without departing from the spirit and scope of the invention as claimed, and that such simple variations and combinations are to be considered as within the scope of the invention as disclosed.

Claims (10)

1. A microbial growth promoting nutritional agent is prepared from the following raw materials: perlite, glucose, yeast extract powder and inorganic metal salt.
2. The microbial growth promoting nutrient according to claim 1, which is prepared from the following raw materials in parts by weight: 100-150 parts of perlite, 50-70 parts of glucose, 8-15 parts of yeast extract powder and 5-15 parts of inorganic metal salt.
3. The microbial growth nutrient according to claim 1, wherein the perlite has a particle size of 100-.
4. The microbial growth nutrient according to claim 3, wherein the perlite comprises a portion with a particle size of 100-400um and a portion with a particle size of 500-800 um.
5. The microorganism growth promoting nutritional agent according to claim 4, wherein the mass ratio of the perlite part with the particle size of 100-400um to the perlite part with the particle size of 500-800um is (3-7): 1.
6. the microbial growth promoting nutrient of claim 2, wherein the inorganic metal salt comprises, by weight, 2.5 to 7 parts of calcium chloride, 1.5 to 3 parts of monopotassium phosphate, 0.6 to 3 parts of magnesium sulfate, 0.2 to 1 part of ferric chloride and 0.2 to 1 part of disodium hydrogen phosphate.
7. The microbial growth promoting nutritional agent of claim 2 further comprising 2-6 parts of B vitamins; the B vitamins are selected from one or more of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B9 and vitamin B12.
8. A process for the preparation of a pro-microbial growth nutrient according to any one of claims 1 to 7, comprising the steps of: adding glucose, yeast extract powder and inorganic metal salt into a reaction container, optionally adding B vitamins, and adding appropriate amount of water to completely dissolve to obtain a mixed solution; grinding perlite, and sieving to obtain powder; mixing the powder with the mixture, stirring, drying, and grinding.
9. Use of a microorganism growth promoting nutrient according to any one of claims 1-7 or prepared according to the preparation method of claim 8 in sewage treatment.
10. The use according to claim 9, wherein the microbial growth promoting nutrient is used for purification treatment of municipal sewage, landfill leachate wastewater, kitchen and kitchen waste wastewater, river black and odorous water body or industrial wastewater.
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