CN113005050A - Composite microbial agent for reducing aspergillus flavus infection and pollution of grain and oil crops as well as preparation method and application of composite microbial agent - Google Patents
Composite microbial agent for reducing aspergillus flavus infection and pollution of grain and oil crops as well as preparation method and application of composite microbial agent Download PDFInfo
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Abstract
The invention discloses a compound microbial agent for reducing aspergillus flavus infection and toxin pollution of grain and oil crops, which comprises the following raw materials in percentage by mass: b, bacillus amyloliquefaciens: 1% -1.5%, enterobacter ludwigii: 2% -2.5%, trichoderma koningii 1: 0.5-1%, Trichoderma koningii 2: 0.5 to 1.5 percent; 0.5 to 1.5 percent of monopotassium phosphate, 1.5 to 2.5 percent of potassium nitrate, 0.5 to 1.5 percent of magnesium sulfate, 45 to 55 percent of wheat bran, 25 to 35 percent of corn flour and 13.5 to 20 percent of meat and bone meal. The compound microbial agent can inhibit fungal diseases such as aspergillus flavus and the like produced in soil, reduce aflatoxin pollution in agricultural products in middle and later periods, improve the quality and quality safety of the agricultural products, and simultaneously, the added multiple trace elements and microorganisms improve the soil fertility, accelerate the degradation of harmful residues in the soil, overcome continuous cropping obstacles of crops and the like.
Description
Technical Field
The invention belongs to the field of functional microorganism application, and particularly relates to a composite microbial agent for reducing aspergillus flavus infection and toxin pollution of grain and oil crops, and a preparation method and application thereof.
Background
Grain and oil crops and products are easily polluted by mycotoxin, particularly aflatoxin, in the growth, storage and transportation processes, and the quality safety and food safety of agricultural products are seriously influenced. How to solve the problem of aflatoxin pollution of agricultural products becomes a worldwide problem to be solved urgently. Aflatoxins are strong carcinogenic and virulent mycotoxins produced by fungi such as aspergillus flavus or aspergillus parasiticus, and can cause pathological changes and even death of organisms after people or animals eat foods or feeds polluted by the toxins, thereby seriously threatening the health and life safety of consumers. Therefore, the prevention and control of aflatoxin pollution in agricultural products such as corn, rice, peanut and the like are not slow.
The pollution of the aflatoxin to agricultural products mainly occurs in the storage period, and the infection of the aflatoxin to crops mainly occurs in the field growth period, so that the strengthening of the prenatal prevention and control of the agricultural products is a key link for inhibiting the aflatoxin pollution. At present, no ideal method exists for preventing and controlling the aspergillus flavus infection of crops in the growing period, and a treatment measure which is healthy to human body, friendly to environment and durable in prevention effect is absolutely necessary to be found. Therefore, the development of aflatoxin pollution biological prevention and control work is becoming a focus and a hot spot of people. The key point of prevention and control of aflatoxin pollution lies in early-stage prevention, and the compound functional microbial agent with good disease prevention effect is a relatively ideal prevention and control method.
The compound microbial agent adopts a modern microbial fermentation technology, high-efficiency functional microbial strains are independently fermented and compounded, cassava residues which are rich in organic matters after activation treatment, livestock manure, humic acid and the like are selected as carriers, and a plurality of nutrient elements are added, so that the compound microbial agent is an environment-friendly fertilizer which can provide plant nutrition for field and economic crops, and has the characteristics of lasting disease prevention, comprehensive nutrition, improvement on crop quality, improvement on chemical fertilizer utilization rate, improvement on soil structure, improvement on soil micro-ecological environment and the like. At present, a plurality of microbial agent products are available on the market, but few compound microbial agent products specially aiming at controlling aspergillus flavus infection and toxin pollution thereof on grain and oil crops are available.
Disclosure of Invention
The technical problem to be solved by the invention is to provide an agricultural microbial agent, which has the effects of preventing and controlling aflatoxin pollution, reducing the application amount of a chemical fertilizer, reducing soil hardening and the like, so that the crops achieve the purposes of high yield, high quality and high efficiency, and the quality and quality safety of agricultural products are improved.
The technical scheme adopted by the invention is as follows:
a composite microbial agent for reducing aspergillus flavus infection and toxin pollution of grain and oil crops comprises the following components in percentage by mass: 1-1.5% of bacillus amyloliquefaciens, 2-2.5% of enterobacter ledwigii, 1% of trichoderma koningii: 0.5-1%, Trichoderma koningii 2: 0.5 to 1.5 percent; 0.5 to 1.5 percent of monopotassium phosphate, 1.5 to 2.5 percent of potassium nitrate, 0.5 to 1.5 percent of magnesium sulfate, 45 to 55 percent of wheat bran, 25 to 35 percent of corn flour and 13.5 to 20 percent of meat and bone meal; wherein, the sum of the mixture ratio of each component contained in the compound microbial agent is 100 percent.
Preferably, the compound microbial agent comprises the following components in percentage by mass: bacillus amyloliquefaciens 1.5%, enterobacter ledwigii 2%, trichoderma koningii 1: 1%, trichoderma koningii 2: 1%, monopotassium phosphate 1%, potassium nitrate 2.5%, magnesium sulfate 1%, wheat bran 50%, corn flour 25% and meat and bone meal 15%.
Among them, Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii) are Trichoderma strains with significantly different growth characteristics isolated from different regions.
The content of viable bacteria in the compound microbial agent is 5 hundred million/g-8 hundred million/g, and the compound microbial agent consists of 4 functional microorganisms: bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Enterobacter ledwigii (Enterobacter ludwigii), Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii), wherein the ratio of the viable count of the 4 microorganisms is 1.5: 2.0: 1.0: 1.0.
in the invention, the Bacillus amyloliquefaciens can be Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JTFM1001 which is preserved in China center for type culture collection (CCTCC for short, with the address of Wuhan university in eight Wuchang district, Wuhan City, China) in 2016, 03 and 28 days, and the preservation number is CCTCC No: m2016145.
In the invention, the Enterobacter ludwigii (Enterobacter ludwigii) BG10-1 has been preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight Wuhan district, Wuhan City, China) at 2016 (7.01.7.2016), and the preservation number is CCTCC No: m2016014.
In the invention, the Trichoderma koningii 1(Trichoderma koningii) TG-72 is preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight roads in Wuhan district, Wuhan city, 2016) in 2016, and the preservation number is CCTCC No: m2016015.
In the invention, the Trichoderma koningii (Trichoderma koningi) TG-102 is preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight roads in Wuhan district, Wuhan city, in 2016, 03 and 28), and the preservation number is CCTCC No: m2016146.
In addition to the above-mentioned deposited strains, they can be obtained by direct purchase from the market, such as the product of Hebei Kangshi Biotech Co., Ltd; the strains such as trichoderma koningii and bacillus amyloliquefaciens can produce enzymes such as chitinase, cellulase and antibacterial protein and other antibacterial substances, have strong inhibiting effect on various plant pathogenic bacteria, and can induce crops to produce disease-resistant effect and improve the quality of agricultural products; the Enterobacter ludwigii can generate a plurality of active substances, activate elements such as phosphorus, potassium, calcium and the like in soil, improve the structure of the soil, break soil hardening and the like; the inventor of the present invention can exert a greater effect by mixing and applying the above 4 kinds of microorganisms in proportion.
The invention also provides a preparation method of the composite microbial agent for preventing and controlling aflatoxin pollution.
The preparation method of the compound microbial agent provided by the invention comprises the following steps:
(1) weighing each strain powder according to the viable count of 4 functional microorganism strains of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Enterobacter Lowenensis (Enterobacter ludwigii), Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii) and mixing uniformly to obtain mixed strain powder;
(2) sterilizing wheat bran, corn flour and meat and bone meal (wet heat sterilization at 121 ℃), and naturally cooling for later use;
(3) uniformly mixing potassium dihydrogen phosphate, potassium nitrate, magnesium sulfate and functional microbial powder in proportion to obtain a mixture;
(4) and (3) uniformly mixing the mixture with the wheat bran, the corn flour and the meat and bone meal treated in the step 2) in proportion, and subpackaging (the production process flow is shown in figure 1).
The compound microbial agent provided by the invention can be used for preventing and controlling the aflatoxin infection or/and toxin pollution of crops.
The crops can be grain and oil crops, including peanuts, corns, rapes, rice and the like.
The prevention and control further refers to the prevention and control of the aflatoxin infection or/and the toxin pollution of the crops in the growing period.
The compound microbial agent provided by the invention can inhibit fungal diseases such as aspergillus flavus and the like produced in soil, and reduce aflatoxin pollution in agricultural products in middle and later periods.
Any of the following applications of the complex microbial inoculant described above also belong to the scope of protection of the present invention:
1) the application as a base fertilizer for crops;
2) the application in preparing crop base fertilizer;
3) the application as the top dressing of crops;
4) the application in preparing crop base fertilizer;
5) the application in preparing the fertilizer for improving the crop yield;
6) the application in crop planting.
The crops are grain and oil crops, including peanuts, corns, rapes, rice and the like.
The composite microbial agent for preventing and controlling the aspergillus flavus infection and the toxin pollution of grain and oil crops can be used as a base fertilizer for being matched with crops such as peanuts, corns, rapes, rice and the like, can be used for independently dressing seeds, dipping roots and irrigating the roots, and can also be used as an additional fertilizer. The base fertilizer is used in 4-8 kg/mu, and the additional fertilizer is used in 4-5 kg/mu. When in application, the plant growth regulator is fully and uniformly mixed with the same amount of fine soil or sand and then applied, can be applied to ground surface ploughing, furrow application or rhizome part of plants in a scattering way, and is watered after application, so that the prevention and control effect on the grain and oil crops aspergillus flavus and the promotion effect on the root system to absorb various nutrient elements are better.
The composite microbial agent containing 4 functional microorganisms and 4 nutrient elements takes wheat bran, corn flour, meat and bone meal and the like as organic carriers, wastes such as leftovers and the like in the agricultural product processing process are fully utilized, the waste of organic resources is reduced, the comprehensive utilization value of substances is improved, and the ecological benefit and the social benefit are remarkable. Meanwhile, the pesticide also has obvious prevention and control effects on aflatoxin pollution in agricultural products, reduces the occurrence of diseases of crops, promotes the good growth of the crops, and improves the physique.
By implementing the specific technical indexes of the invention, the following beneficial effects can be achieved:
1. the functional compound microbial agent is prepared by scientific compounding by a method of adding the microbial agent, wherein the number of effective viable bacteria can reach 5 hundred million/g, and the functional compound microbial agent is rich in organic matters and various elements such as quick-acting N, P, K, Mg required by crop growth, does not contain toxic and harmful substances such as heavy metals, and has small volume weight and good air permeability.
2. The selected functional microorganisms and the plant root system form a symbiotic system, so that the absorption and utilization of mineral nutrient elements by plants can be promoted, the plant nutrient condition can be improved, the most obvious effect is that the absorption and utilization of various elements in soil by the plants can be improved, the utilization rate of fertilizers is improved, and the yield and the quality of crops are improved; enhancing the stress resistance of the plants.
3. The field test result shows that under the condition of maintaining the same yield, the microbial agent can obviously reduce the using amount of the fertilizer and the pollution degree of aflatoxin after being applied, and the cost is saved. Meanwhile, the residual quantity and the pollution degree of the fertilizer can be reduced, the quality of agricultural products is obviously improved, the quality safety and the food safety degree of the agricultural products are improved, and the ecological benefit, the economic benefit and the social benefit are good.
Biological material preservation instructions
The strain name is as follows: bacillus amyloliquefaciens
Latin name: bacillus amyloliquefaciens
The strain number is as follows: JTFM1001
The preservation organization: china center for type culture Collection
The preservation organization is abbreviated as: CCTCC (China center for cell communication)
Address: wuhan university in eight roads of Wuchang district in Wuhan City of China
The preservation date is as follows: 2016, 03 and 28 days
Registration number of the preservation center: CCTCC No: m2016145
Biological material preservation instructions
The strain name is as follows: enterobacter ludwigii
Latin name: enterobacter ludwigii
The strain number is as follows: BG10-1
The preservation organization: china center for type culture Collection
The preservation organization is abbreviated as: CCTCC (China center for cell communication)
Address: wuhan university in eight roads of Wuchang district in Wuhan City of China
The preservation date is as follows: 2016 (01 month and 7 days)
Registration number of the preservation center: CCTCC No: m2016014
Biological material preservation instructions
The strain name is as follows: trichoderma koningii
Latin name: trichoderma koningi
The strain number is as follows: TG-72
The preservation organization: china center for type culture Collection
The preservation organization is abbreviated as: CCTCC (China center for cell communication)
Address: wuhan university in eight roads of Wuchang district in Wuhan City of China
The preservation date is as follows: 2016 (01 month and 7 days)
Registration number of the preservation center: CCTCC No: m2016015
Biological material preservation instructions
The strain name is as follows: trichoderma koningii
Latin name: trichoderma koningi
The strain number is as follows: TG-102
The preservation organization: china center for type culture Collection
The preservation organization is abbreviated as: CCTCC (China center for cell communication)
Address: wuhan university in eight roads of Wuchang district in Wuhan City of China
The preservation date is as follows: 2016, 03 and 28 days
Registration number of the preservation center: CCTCC No: m2016146
Drawings
FIG. 1 is a flow chart of the production process for preparing the powdery agricultural microbial agent.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples, but the present invention is not limited to the following examples. The method is a conventional method unless otherwise specified. The starting materials are commercially available from the open literature unless otherwise specified.
The conventional compound fertilizer used in the following examples was purchased from Strobilanthes chemical fertilizer, Inc., and the nitrogen, phosphorus and potassium contents of the product were 16-5-30.
The Bacillus amyloliquefaciens used in the following examples is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JTFM1001 which is preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight roads of Wuchang district, Wuhan city, China) in 2016, 03 and 28 days, and the preservation number is CCTCC No: m2016145.
The Enterobacter ludwigii used in the following examples is Enterobacter ludwigii BG10-1 which has been deposited in China center for type culture Collection (CCTCC, address: Wuhan university, eighty-one way Wuhan district, Wuchang city, China) at 2016 (7.01.7.2016), with the preservation number of CCTCC No: m2016014.
Trichoderma koningii 1 used in the following examples is Trichoderma koningii TG-72, which was deposited in China center for type culture Collection (CCTCC, address: Wuhan university, eighty one way of Wuhan district, Wuhan City, 2016) at 7.01.2016, with the collection number of CCTCC No: m2016015.
Trichoderma koningii 2 used in the following examples is Trichoderma koningii (Trichoderma koningi) TG-102, which was deposited in China center for type culture Collection (CCTCC, address: Wuhan university, eight roads of Wuhan district, Wuhan City, USA, 2016 and 03/28/2016, with the preservation number of CCTCC No: m2016146.
Example 1, the complex microbial inoculant for reducing aspergillus flavus infection and toxin pollution of grain and oil crops and the preparation thereof the complex microbial inoculant of the present example is composed of the following raw materials by mass percent:
b, bacillus amyloliquefaciens: 1.5%, enterobacter ledwigii: 2%, trichoderma koningii 1: 1%, trichoderma koningii 2: 1%, monopotassium phosphate 1%, potassium nitrate 2.5%, magnesium sulfate 1%, wheat bran 50%, corn flour 25% and meat and bone meal 15%. Wherein, the viable count content of the obtained compound microbial inoculum is 5 hundred million/g, and the compound microbial inoculum consists of 4 functional microorganisms: the ratio of viable count of the bacillus amyloliquefaciens, the enterobacter ledwigii, the trichoderma koningii 1 and the trichoderma koningii 2 is 1.5: 2.0: 1.0: 1.0.
the preparation method comprises the following steps:
(1) weighing each biocontrol bacterium powder according to the viable count of the 4 functional microorganism bacterium powders and then uniformly mixing;
(2) sterilizing testa Tritici, semen Maydis powder and meat and bone meal (121 deg.C moist heat sterilization), and naturally cooling;
(3) uniformly mixing potassium dihydrogen phosphate, potassium nitrate, magnesium sulfate and functional microbial powder in proportion;
(4) mixing the above mixture with testa Tritici, semen Maydis powder and meat and bone meal at a certain proportion, and packaging.
Effect test:
in 2016, field experiments are carried out in bases of agricultural departments of camphor tree city in Jiangxi province from 3 months to 8 months, and base fertilizers are used for manual spreading before sowing. 50 kg of conventional compound fertilizer is applied to the control group, 50 kg of conventional compound fertilizer and 4 kg of compound microbial agent are applied to the experimental group 1; the experiment group 2 applies 50 kg of conventional compound fertilizer and 8 kg of compound microbial agent, and the agronomic measures such as fertilizer, water, pesticide, management and the like during the growth period of the peanuts are consistent.
The statistics of test results shows that the yield of the experimental group 1 is increased by 9.5 percent compared with the control group, and the pollution prevention and control effect of the aflatoxin reaches 68.5 percent; the experimental group 2 has a 13.5% yield increase compared with the control group, and the aflatoxin pollution prevention and control effect reaches 73.5%. Compared with the results of 2 groups of experiments, the functional compound microbial agent can improve the crop yield and reduce the aflatoxin pollution rate.
The prevention and control effect is the pollution amount of the control area, the pollution amount of prevention and control treatment/the pollution amount of the control area
Example 2, the complex microbial inoculant for reducing aspergillus flavus infection and toxin pollution of grain and oil crops and the preparation thereof the complex microbial inoculant of the present example is composed of the following raw materials by mass percent:
b, bacillus amyloliquefaciens: 1.5%, enterobacter ledwigii: 2%, trichoderma koningii 1: 1%, trichoderma koningii 2: 1%, monopotassium phosphate 1%, potassium nitrate 2.5%, magnesium sulfate 1%, wheat bran 50%, corn flour 25% and meat and bone meal 15%. Wherein, the viable count content of the obtained compound microbial inoculum is 8 hundred million/g, and the compound microbial inoculum consists of 4 functional microorganisms: the ratio of viable count of the bacillus amyloliquefaciens, the enterobacter ledwigii, the trichoderma koningii 1 and the trichoderma koningii 2 is 1.5: 2.0: 1.0: 1.0.
the preparation method comprises the following steps:
(1) weighing each biocontrol bacterium powder according to the viable count of the 4 functional microorganism bacterium powders and then uniformly mixing;
(2) sterilizing testa Tritici, semen Maydis powder and meat and bone meal (121 deg.C moist heat sterilization), and naturally cooling;
(3) uniformly mixing potassium dihydrogen phosphate, potassium nitrate, magnesium sulfate and functional microbial powder in proportion;
(4) mixing the above mixture with testa Tritici, semen Maydis powder and meat and bone meal at a certain proportion, and packaging
Effect test:
in 2016, the field test is carried out in Tangshan base of environmental protection, scientific research and monitoring institute in rural areas, and base fertilizer is applied by manual spreading before sowing. 50 kilograms of conventional compound fertilizer is applied to each mu of the control group, 50 kilograms of conventional compound fertilizer and 4 kilograms of compound microbial agent are applied to the experiment group 1; the experiment group 2 is applied with 50 kg of conventional compound fertilizer and 8 kg of compound microbial agent, and the agronomic measures such as fertilizer, water, pesticide and management during the growth period of the peanuts are consistent.
The statistics of test results shows that the yield of the experimental group 1 is increased by 12.5 percent compared with the control group, and the aflatoxin pollution rate is reduced by 75.5 percent; compared with the control group, the experimental group 2 has the advantages that the yield is increased by 15.5 percent, and the aflatoxin pollution rate is reduced by 83.5 percent. Compared with 2 groups of experimental results, the functional compound microbial agent can obviously improve the crop yield and reduce the aflatoxin pollution rate.
Example 3, the complex microbial inoculant for reducing aspergillus flavus infection and toxin pollution of grain and oil crops and the preparation thereof the complex microbial inoculant of the present example consists of the following raw materials in percentage by mass:
b, bacillus amyloliquefaciens: 1.5%, enterobacter ledwigii: 2%, trichoderma koningii 1: 1%, trichoderma koningii 2: 1%, monopotassium phosphate 1%, potassium nitrate 2.5%, magnesium sulfate 1%, wheat bran 50%, corn flour 25% and meat and bone meal 15%. Wherein, the viable count content of the obtained compound microbial inoculum is 8 hundred million/g, and the compound microbial inoculum consists of 4 functional microorganisms: the ratio of viable count of the bacillus amyloliquefaciens, the enterobacter ledwigii, the trichoderma koningii 1 and the trichoderma koningii 2 is 1.5: 2.0: 1.0: 1.0.
the preparation method comprises the following steps:
(1) weighing each biocontrol bacterium powder according to the viable count of the 4 functional microorganism bacterium powders and then uniformly mixing;
(2) performing moist heat sterilization on wheat bran, corn flour, meat and bone meal and the like at 121 ℃, and naturally cooling for later use;
(3) uniformly mixing potassium dihydrogen phosphate, potassium nitrate, magnesium sulfate and functional microbial powder in proportion;
(4) mixing the above mixture with testa Tritici, semen Maydis powder and meat and bone meal at a certain proportion, and packaging
Effect test:
in 2016, 3-10 months, the fertilizer is tested in the Yangho base of the institute of oil crops, Chinese academy of agricultural sciences, and is used as a base fertilizer before sowing. 50 kg of conventional compound fertilizer is applied to each mu of the control group, 50 kg of conventional compound fertilizer and 8 kg of compound microbial agent are applied to the experimental group 1; the experimental group 2 is applied with 60 kg of conventional compound fertilizer and 8 kg of compound microbial agent, and the agronomic measures such as fertilizer, water, pesticide and management during the growth period of the corn are consistent.
The statistics of test results shows that the yield of the experimental group 1 is increased by 13.5 percent compared with the control group, and the aflatoxin pollution rate is reduced by 64.5 percent; compared with the control group, the experimental group 2 has the advantages that the yield is increased by 14.8 percent, and the aflatoxin pollution rate is reduced by 66.7 percent. Compared with 2 groups of experimental results, the composite microbial agent can obviously reduce aflatoxin pollution and improve the quality of agricultural products, but the crop yield is not obviously increased along with the increase of the application amount of the fertilizer.
Claims (10)
1. A compound microbial agent comprises the following components in percentage by mass: b, bacillus amyloliquefaciens: 1-1.5%, 2-2.5% of enterobacter ledwigii, 1% of trichoderma koningii: 0.5-1%, Trichoderma koningii 2: 0.5 to 1.5 percent; 0.5 to 1.5 percent of monopotassium phosphate, 1.5 to 2.5 percent of potassium nitrate, 0.5 to 1.5 percent of magnesium sulfate, 45 to 55 percent of wheat bran, 25 to 35 percent of corn flour and 13.5 to 20 percent of meat and bone meal; wherein, the sum of the mixture ratio of each component contained in the compound microbial agent is 100 percent.
2. The complex microbial inoculant according to claim 1, wherein: the compound microbial agent comprises the following components in percentage by mass: bacillus amyloliquefaciens 1.5%, enterobacter ledwigii 2%, trichoderma koningii 1: 1%, trichoderma koningii 2: 1 percent; 1% of monopotassium phosphate, 2.5% of potassium nitrate, 1% of magnesium sulfate, 50% of wheat bran, 25% of corn flour and 15% of meat and bone meal.
3. The complex microbial inoculant according to claim 1 or 2, wherein: the content of viable bacteria in the compound microbial agent is 5 hundred million/g-8 hundred million/g; the ratio of the viable count of the bacillus subtilis, the bacillus amyloliquefaciens, the enterobacter ledebei and the bacillus cereus in the composite microbial agent is 1.5: 2.0: 1.0: 1.0.
4. the method for preparing the complex microbial inoculant of any one of claims 1-3, comprising the following steps:
(1) the viable count of each bacterial powder of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Enterobacter lodesignans (Enterobacter ludwigii), Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii) according to any one of claims 1 to 3 is weighed according to a proportion and then uniformly mixed to obtain mixed bacterial powder;
(2) sterilizing wheat bran, corn flour and meat and bone meal, and naturally cooling for later use;
(3) uniformly mixing potassium dihydrogen phosphate, potassium nitrate, magnesium sulfate and functional microbial powder in proportion to obtain a mixture;
(4) uniformly mixing the mixture obtained in the step 3) with the wheat bran, the corn flour and the meat and bone meal treated in the step 2) according to a proportion.
5. The use of the complex microbial inoculant defined in any one of claims 1 to 3 in 1) to 6) below:
1) the application as a base fertilizer for crops;
2) the application in preparing crop base fertilizer;
3) the application as the top dressing of crops;
4) the application in preparing crop base fertilizer;
5) the application in preparing the fertilizer for improving the crop yield;
6) the application in crop planting.
6. Use according to claim 5, characterized in that: the crops are grain and oil crops, including peanut, corn, rape and rice.
7. The use of the complex microbial inoculant defined in any one of claims 1 to 3 for controlling aflatoxin infestation or/and toxin contamination of crops.
8. Use according to claim 7, characterized in that: the crops are grain and oil crops, including peanuts, corns, rapes and rice;
the prevention and control refers to the prevention and control of the aflatoxin infection or/and the toxin pollution in the growing period of crops.
9. The method for using a complex microbial inoculant according to any one of claims 1 to 3, wherein the complex microbial inoculant is used as a base fertilizer for crops or/and the complex microbial inoculant is used as a top fertilizer for crops.
10. Use according to claim 9, characterized in that: the crops are grain and oil crops, including peanuts, corns, rapes and rice;
the base fertilizer is used in an amount of 4-8 kg per mu, and the additional fertilizer is used in an amount of 4-5 kg per mu.
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| CN114097459A (en) * | 2021-10-19 | 2022-03-01 | 中国农业科学院油料作物研究所 | Method for preventing and controlling aspergillus flavus and toxin thereof and increasing number of root nodules with nitrogenase activity of leguminous crops and application of method |
| CN114196572A (en) * | 2021-11-15 | 2022-03-18 | 中国农业科学院油料作物研究所 | Microbial agent with aflatoxin, toxigenic bacteria prevention and control and crop yield increase promotion and application thereof |
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| CN114097459A (en) * | 2021-10-19 | 2022-03-01 | 中国农业科学院油料作物研究所 | Method for preventing and controlling aspergillus flavus and toxin thereof and increasing number of root nodules with nitrogenase activity of leguminous crops and application of method |
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| CN114196572B (en) * | 2021-11-15 | 2023-09-26 | 中国农业科学院油料作物研究所 | Microbial agent with aflatoxin and toxigenic bacteria prevention and control and crop yield increase promotion functions and application thereof |
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