CN113004095A - Composite microbial fertilizer for preventing and controlling aflatoxin pollution and preparation method thereof - Google Patents
Composite microbial fertilizer for preventing and controlling aflatoxin pollution and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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Abstract
The invention discloses a composite microbial fertilizer for preventing and controlling peanut aflatoxin pollution and a preparation method thereof, and discloses a composite microbial fertilizer containing 4 functional bacteria for preventing and controlling aflatoxin pollution and 4 trace elements, wherein cassava residue and peanut straw are used as carriers. The microbial fertilizer can effectively reduce aflatoxin pollution in grain and oil agricultural products, improve the quality safety of the agricultural products and food, improve soil fertility, improve soil structure, improve the stress resistance of crops, increase production and income, and obviously improve ecological benefits and social benefits.
Description
Technical Field
The invention belongs to the field of beneficial microorganisms and fertilizer application, and particularly relates to a compound microbial fertilizer for preventing and controlling aflatoxin pollution and a preparation method thereof.
Background
Aflatoxins are strong carcinogenic and virulent mycotoxins produced by fungi such as aspergillus flavus or aspergillus parasiticus, and can cause pathological changes and even death of organisms after people or animals eat foods or feeds polluted by the toxins, thereby seriously threatening the health and life safety of consumers. Therefore, the prevention and control of aflatoxin pollution in agricultural products such as peanuts, corns and rice are not slow. The infection of the aspergillus flavus is mainly in the growth period of crops, and the aflatoxin pollution mainly occurs in the storage period of agricultural products, so that the strengthening of prenatal prevention and control of the agricultural products is a key link for inhibiting the aflatoxin pollution. At present, chemical control occupies an important position in the control of aflatoxin pollution of crops, the chemical control has high cost and is easy to pollute the environment, and meanwhile, pathogenic bacteria are easy to generate drug resistance. Therefore, the development of aflatoxin pollution biological prevention and control work is becoming a focus and a hot spot of people. The key point of prevention and control of aflatoxin pollution lies in early-stage prevention, so that the aflatoxin pollution prevention and control method can be used as a base fertilizer or an additional fertilizer, and the compound functional microbial fertilizer with good disease prevention effect is an ideal control method.
The compound microbial fertilizer adopts a modern microbial fermentation technology, high-efficiency microbial strains are independently fermented and compounded, cassava residues which are rich in organic matters after activation treatment, livestock manure, humic acid and the like are selected as carriers, and a plurality of nutrient elements are added, so that the compound microbial fertilizer is an environment-friendly fertilizer which can provide plant nutrition for field and economic crops, and has the characteristics of comprehensive nutrition, lasting fertilizer effect, improvement on crop quality, improvement on chemical fertilizer utilization rate, improvement on soil structure, improvement on soil micro-ecological environment and the like. At present, a plurality of composite microbial fertilizer products are available in the market, but the microbial fertilizer products specially aiming at preventing and controlling aflatoxin pollution on crops are very few, the application cost is high, and the effect is unstable.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a composite microbial fertilizer for preventing and controlling aflatoxin pollution and a preparation method thereof. The microbial fertilizer contains 4 functional microorganisms and 5 trace elements, cassava dregs and peanut straw powder are used as carriers, the special fertilizer can better prevent and control the pollution of aflatoxin in crops or products, the application amount of the fertilizer is reduced, the disease resistance of the crops is improved, and the quality and the yield of agricultural products can be obviously increased.
The technical scheme adopted by the invention is as follows:
a composite microbial fertilizer for preventing and controlling aflatoxin pollution comprises the following raw materials in parts by mass:
2-5 parts of ammonium molybdate, 3-7 parts of potassium fulvate, 0.5-1 part of zinc sulfate, 0.1-0.2 part of borax, 3-5 parts of citric acid chelated manganese, 5-10 parts of a compound functional microbial agent, 35-50 parts of cassava residue and 25-35 parts of peanut straw powder.
The inventor further optimizes the mixture ratio of the raw materials, and the compound microbial fertilizer comprises the following raw materials in parts by mass: 2-3 parts of ammonium molybdate, 3-4 parts of potassium fulvate, 0.8 part of zinc sulfate, 0.2 part of borax, 5 parts of citric acid chelated manganese, 8 parts of composite functional microbial powder, 45 parts of cassava residue and 35 parts of corn stalk powder.
The composite microbial fertilizer has a viable count of 0.5-1 hundred million/gram, and consists of 4 functional microorganisms: bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Enterobacter ludwigii (Enterobacter ludwigii), Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii), wherein the ratio of viable count of 4 microorganisms is 1:1:1:1 or 2:2:3: 3. Among them, Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii) are different Trichoderma strains isolated in different regions and having significant differences in growth characteristics.
In one embodiment of the invention, the viable count of the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the Enterobacter Lowenensis (Enterobacter ludwigii), the Trichoderma koningii 1(Trichoderma koningi) and the Trichoderma koningii 2(Trichoderma koningi) in the composite microbial fertilizer is 2500 ten thousand cfu/g, 2500 ten thousand cfu/g and 2500 ten thousand cfu/g respectively.
In another embodiment of the invention, the viable count of the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the Enterobacter Lowenensis (Enterobacter ludwigii), the Trichoderma koningii 1(Trichoderma koningi) and the Trichoderma koningii 2(Trichoderma koningi) in the composite microbial fertilizer is 2000 ten thousand cfu/g, 3000 ten thousand cfu/g and 3000 ten thousand cfu/g respectively.
In the invention, the Bacillus amyloliquefaciens can be Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JTFM1001 which is preserved in China center for type culture collection (CCTCC for short, with the address of Wuhan university in eight Wuchang district, Wuhan City, China) in 2016, 03 and 28 days, and the preservation number is CCTCC No: m2016145.
In the invention, the Enterobacter ludwigii (Enterobacter ludwigii) BG10-1 has been preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight Wuhan district, Wuhan City, China) at 2016 (7.01.7.2016), and the preservation number is CCTCC No: m2016014.
In the invention, the Trichoderma koningii 1(Trichoderma koningii) TG-72 is preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight roads in Wuhan district, Wuhan city, 2016) in 2016, and the preservation number is CCTCC No: m2016015.
In the invention, the Trichoderma koningii (Trichoderma koningi) TG-102 is preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight roads in Wuhan district, Wuhan city, in 2016, 03 and 28), and the preservation number is CCTCC No: m2016146.
In addition to the above-mentioned deposited strains, they can be obtained by direct purchase from the market, such as the product of Hebei Kangshi Biotech Co., Ltd; wherein, the strains of bacillus amyloliquefaciens and the like can generate enzymes such as chitinase, antibacterial protein and the like and other antibacterial substances, and have strong inhibiting effect on various plant pathogenic bacteria; the Enterobacter ludwigii can generate a plurality of active substances, activate elements such as phosphorus, potassium, calcium and the like in soil, improve the structure of the soil, break soil hardening and the like; the trichoderma koningii has the capacity of inhibiting the growth of pathogenic bacteria and inducing crops to generate disease-resistant effect; the inventor mixes and applies the functional microorganisms according to the proportion and can play a greater role.
The potassium fulvate is also called potassium fulvate, and the mixture of natural aromatic carboxylic acid extracted from peat, lignite or weathered coal by Fulvic Acid (FA) can form a coordination bond with metal cations, namely a complex or a chelate is formed, thus being beneficial to absorption and utilization of various beneficial elements by plants, and further improving the utilization rate of nutrients. The fulvic acid and potassium element are chelated to form fulvic acid chelated potassium, so that the growth of harmful pathogenic bacteria can be inhibited, and the occurrence of soil-borne diseases is reduced to the maximum extent.
The citric acid chelated manganese is used as a trace element nutrient, is used for agriculture, forestry, animal husbandry or other economic crops, and can be widely applied to water soluble fertilizers, foliar fertilizers, plant nutrient regulators and chelated compound fertilizers. The manganese element fertilizer has the following characteristics: 1. can promote the seeds to mature in advance and improve the maturing rate. 2. The citric acid fully chelates metal elements and organic active substances, so that the compatibility problem is effectively solved, the citric acid, metal ions and the organic active substances can be slowly released in crops, the slow release effect is obvious, and the absorption and utilization rate is high. 3. The citric acid and the organic active substances are slowly released, the pH value of the soil environment can be adjusted to be slightly acidic, the soil environment beneficial to the activity of microorganisms can be formed, a good soil aggregate structure is formed, and the soil improvement effect is obvious.
The cassava residues are byproducts of industrial production, the peanut straws are byproducts of agricultural production, are rich in various nutritional ingredients such as crude protein, crude fat, crude fiber and crude ash, and can meet the requirements of crops on organic nutrition in growth and development; the product has low content of toxic and harmful substances, can reduce environmental pollution, is green and environment-friendly, and is an important raw material for producing green organic agricultural products; the high-quality organic fertilizer can be produced through processing, the economic benefit of the planting industry is provided, and the method has great development potential in the production and application of the organic fertilizer.
The invention also provides a preparation method of the composite microbial fertilizer for preventing and controlling peanut aflatoxin pollution.
The preparation form of the compound microbial fertilizer prepared by the invention is powder, and the preparation steps are as follows:
(1) weighing each biocontrol bacterium powder according to the viable count of 4 functional microorganism bacterium powders of bacillus subtilis, bacillus amyloliquefaciens, enterobacter ludwigii and trichoderma koningii, and uniformly mixing to obtain mixed bacterium powder;
(2) sterilizing the cassava residue and the peanut straw powder, and naturally cooling for later use;
(3) uniformly mixing ammonium molybdate, zinc sulfate, borax, potassium fulvate, manganese citrate and the mixed bacteria powder in proportion to obtain a mixture;
(4) and (3) uniformly mixing the mixture with the cassava residue and the peanut straw powder treated in the step (2) in proportion, and subpackaging (the production process flow is shown in figure 1).
The composite microbial fertilizer provided by the invention can be used for preventing and controlling the aflatoxin infection or/and toxin pollution of crops.
The crops can be grain and oil crops, including peanuts, corns, rapes, rice and the like.
The prevention and control further refers to the prevention and control of the aflatoxin infection or/and the toxin pollution of the crops in the growing period.
Any one of the following applications of the compound microbial fertilizer also belongs to the protection scope of the invention:
1) the application as a base fertilizer for crops;
2) the application in preparing crop base fertilizer;
3) the application as the top dressing of crops;
4) the application in preparing crop base fertilizer;
5) the application in preparing the fertilizer for improving the crop yield;
6) the application in crop planting.
The crops are grain and oil crops, including peanuts, corns, rapes, rice and the like.
The compound microbial fertilizer for preventing and controlling aflatoxin pollution provided by the invention can be applied together with base fertilizers of crops such as peanuts, corns, rapes, rice and the like, and also can be applied as top dressing, wherein the using amount of the base fertilizer per mu is 20-40 kg, and the using amount of the top dressing per mu is 10-20 kg. When in use, the fertilizer is fully and uniformly mixed with the same amount of fine soil or sand and then applied, and can be applied to ground surface ploughing, furrow hole application or rhizome parts of plants in a scattering way, and watering is carried out after application, so that the prevention and control effect on crop diseases is better.
The compound microbial fertilizer containing 4 functional microorganisms and 5 trace elements takes the cassava residues and the peanut straw powder as the organic carriers, fully utilizes leftovers in the cassava fermentation process and waste utilization of the peanut straws, reduces waste of organic resources, reduces pollution to the environment, improves comprehensive utilization value of substances, and has remarkable ecological and social benefits. Meanwhile, the pesticide also has obvious prevention and control effects on aflatoxin pollution in agricultural products, reduces the occurrence of diseases of crops, promotes the good growth of the crops, improves the quality and enhances the efficiency.
Biological material preservation instructions
The strain name is as follows: bacillus amyloliquefaciens
Latin name: bacillus amyloliquefaciens
The strain number is as follows: JTFM1001
The preservation organization: china center for type culture Collection
The preservation organization is abbreviated as: CCTCC (China center for cell communication)
Address: wuhan university in eight roads of Wuchang district in Wuhan City of China
The preservation date is as follows: 2016, 03 and 28 days
Registration number of the preservation center: CCTCC No: m2016145
Biological material preservation instructions
The strain name is as follows: enterobacter ludwigii
Latin name: enterobacter ludwigii
The strain number is as follows: BG10-1
The preservation organization: china center for type culture Collection
The preservation organization is abbreviated as: CCTCC (China center for cell communication)
Address: wuhan university in eight roads of Wuchang district in Wuhan City of China
The preservation date is as follows: 2016 (01 month and 7 days)
Registration number of the preservation center: CCTCC No: m2016014
Biological material preservation instructions
The strain name is as follows: trichoderma koningii
Latin name: trichoderma koningi
The strain number is as follows: TG-72
The preservation organization: china center for type culture Collection
The preservation organization is abbreviated as: CCTCC (China center for cell communication)
Address: wuhan university in eight roads of Wuchang district in Wuhan City of China
The preservation date is as follows: 2016 (01 month and 7 days)
Registration number of the preservation center: CCTCC No: m2016015
Biological material preservation instructions
The strain name is as follows: trichoderma koningii
Latin name: trichoderma koningi
The strain number is as follows: TG-102
The preservation organization: china center for type culture Collection
The preservation organization is abbreviated as: CCTCC (China center for cell communication)
Address: wuhan university in eight roads of Wuchang district in Wuhan City of China
The preservation date is as follows: 2016, 03 and 28 days
Registration number of the preservation center: CCTCC No: m2016146
Drawings
FIG. 1 is a flow chart of the production process of the powdery bacterial manure.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples, but the present invention is not limited to the following examples. The method is a conventional method unless otherwise specified. The starting materials are commercially available from the open literature unless otherwise specified. The quantitative tests in the following examples, all set up three replicates and the results averaged.
The conventional compound fertilizer used in the following examples was purchased from Strobilanthes chemical fertilizer, Inc., and the nitrogen, phosphorus and potassium contents of the product were 16-5-30.
In the following examples, the Bacillus amyloliquefaciens may be Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JTFM1001, which has been deposited in the china type culture collection center (CCTCC, address: eight-way university of wuhan district, wuhan city, china) at 03/28 th 2016 with the collection number of CCTCC No: m2016145.
The Enterobacter ludwigii can be Enterobacter ludwigii BG10-1 which is preserved in China center for type culture Collection (CCTCC for short, with the address of eight-way Wuhan university in Wuchang district, Wuhan city, China) in 2016 (01.7.01.7.M.) with the preservation number of CCTCC No: m2016014.
The Trichoderma koningii 1 can be Trichoderma koningii 1(Trichoderma koningi) TG-72 which is preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight roads of Wuhan district, Wuhan City, 2016) in 7 th month in 2016, and the preservation number is CCTCC No: m2016015.
The Trichoderma koningii 2 can be Trichoderma koningii 2(Trichoderma koningi) TG-102 which is preserved in China center for type culture Collection (CCTCC for short, address: Wuhan university, eight roads of Wuhan district, Wuhan city, 2016) in 28 th of 2016, and the preservation number is CCTCC No: m2016146.
Example 1 composite microbial fertilizer for preventing and controlling peanut aflatoxin pollution and preparation thereof
The compound microbial fertilizer of the embodiment comprises the following raw materials in parts by mass:
3 parts of ammonium molybdate, 3 parts of potassium fulvate, 0.8 part of zinc sulfate, 0.2 part of borax, 5 parts of citric acid chelated manganese, 8 parts of composite functional microbial powder, 45 parts of cassava residue and 35 parts of peanut straw powder.
The number of viable bacteria in the composite microbial fertilizer is 1.0 hundred million/gram, wherein the number of viable bacteria of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Enterobacter Lophatheri (Enterobacter ludwigii), Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii) is 2500 ten thousand cfu/g, 2500 ten thousand cfu/g and 2500 ten thousand cfu/g respectively.
The preparation method comprises the following steps:
(1) weighing each biocontrol bacterium powder according to the viable count of the 4 functional microorganism bacterium powders and then uniformly mixing;
(2) sterilizing the cassava residue and the peanut straw powder (sterilizing at 121 ℃ in a moist heat mode), and naturally cooling for later use;
(3) and uniformly mixing ammonium molybdate, potassium fulvate, citric acid chelated manganese and functional microbial powder in proportion. Obtaining a mixture;
(4) and (3) uniformly mixing the mixture with the cassava residue and the peanut straw powder in the step (2) in proportion, and subpackaging.
Effect test:
in 2016, performing field test on a base of Tangshan city of Hebei province \28390, county Baixin peanut planting cooperative society in 3-10 months, applying the fertilizer before sowing, applying 50 kg of a conventional compound fertilizer to a control group per mu, and applying 50 kg of the conventional compound fertilizer and 20 kg of a compound microbial fertilizer to an experimental group 1; the experimental group 2 applied 60 kg of conventional compound fertilizer and 20 kg of compound microbial fertilizer, and the agronomic measures such as fertilizer, water, pesticide and management during the growth period of peanuts were consistent.
The statistics of test results shows that the yield of the experimental group 1 is increased by 10.5 percent compared with the control group, and the pollution prevention and control effect of the aflatoxin reaches 75.8 percent; the yield of the experimental group 2 is increased by 11.5 percent compared with that of the control group, the aflatoxin pollution prevention and control effect is 76.9 percent, and the yield of the peanuts is not obviously increased even under the condition of increasing the dosage of the conventional fertilizer through comparison of the experimental results of the 2 groups.
The prevention and control effect is the pollution amount of the control area, the pollution amount of prevention and control treatment/the pollution amount of the control area
Example 2, composite microbial fertilizer for preventing and controlling peanut aflatoxin pollution and preparation thereof
The compound microbial fertilizer of the embodiment comprises the following raw materials in parts by mass:
2 parts of ammonium molybdate, 4 parts of potassium fulvate, 0.8 part of zinc sulfate, 0.2 part of borax, 5 parts of citric acid chelated manganese, 8 parts of composite functional microbial powder, 45 parts of cassava residue and 35 parts of peanut straw powder.
The number of viable bacteria in the composite microbial fertilizer is 1.0 hundred million/gram, wherein the ratio of the number of viable bacteria of 4 kinds of microorganisms is 2:2:3: 3.
The preparation method comprises the following steps:
(1) weighing each biocontrol bacterium powder according to the viable count of the 4 functional microorganism bacterium powders and then uniformly mixing;
(2) sterilizing the cassava residue and the peanut straw powder (sterilizing at 121 ℃ in a moist heat mode), and naturally cooling for later use;
(3) and uniformly mixing ammonium molybdate, potassium fulvate, citric acid chelated manganese and functional microbial powder in proportion. Obtaining a mixture;
(4) and (3) uniformly mixing the mixture with the cassava residue and the peanut straw powder in the step (2) in proportion, and subpackaging.
Effect test:
in 2016, performing field test on Cinnamomum camphora test base in Jiangxi of oil crop research institute of Chinese academy of agricultural sciences in 3-8 months, applying 50 kg of conventional compound fertilizer to each acre of a control group, applying 50 kg of conventional compound fertilizer and 20 kg of compound microbial fertilizer to an experimental group 1, and manually spreading the two fertilizers; the experimental group 2 applied 60 kg of conventional compound fertilizer and 20 kg of compound microbial fertilizer, and both fertilizers applied by manual spreading. The peanut growth period is consistent with the agronomic measures of fertilizer, water, pesticide, management and the like.
The statistics of test results shows that the yield of the experimental group 1 is increased by 12.5 percent compared with the control group, and the pollution prevention and control effect of the aflatoxin is 71.5 percent; the experimental group 2 has 12.8 percent of yield increase compared with the control group, and the aflatoxin pollution prevention and control effect is 70.8 percent; through comparison of 2 groups of experimental results, the yield of the peanuts is not remarkably increased even under the condition of increasing the using amount of the fertilizer.
Claims (10)
1. The compound microbial fertilizer comprises the following raw materials in parts by mass: 2-5 parts of ammonium molybdate, 3-7 parts of potassium fulvate, 0.5-1 part of zinc sulfate, 0.1-0.2 part of borax, 3-5 parts of citric acid chelated manganese, 5-10 parts of a compound functional microbial agent, 35-50 parts of cassava residue and 25-35 parts of peanut straw powder.
2. The composite microbial fertilizer as claimed in claim 1, wherein: the compound microbial fertilizer comprises the following raw materials in parts by mass: 2-3 parts of ammonium molybdate, 3-4 parts of potassium fulvate, 0.8 part of zinc sulfate, 0.2 part of borax, 5 parts of citric acid chelated manganese, 8 parts of composite functional microbial powder, 45 parts of cassava residue and 35 parts of corn stalk powder.
3. The composite microbial fertilizer according to claim 1 or 2, wherein: the content of viable bacteria in the compound microbial fertilizer is 0.5-1 hundred million/g; in the composite microbial fertilizer, the ratio of viable bacteria of 4 microorganisms is 1:1:1 or 2:2:3:3 in sequence, wherein the ratio of viable bacteria of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Enterobacter ledwigii (Enterobacter ludwigii), Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii) is respectively 1:1:1: 1.
4. A process for the preparation of a composite microbial fertilizer as claimed in any one of claims 1 to 3, comprising the steps of:
(1) the method comprises the steps of weighing the bacterial powder viable count of each bacterium according to the ratio of the bacterial powder of 4 kinds of bacteria, namely Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Enterobacter lodesignans (Enterobacter ludwigii), Trichoderma koningii 1(Trichoderma koningii) and Trichoderma koningii 2(Trichoderma koningii) according to any one of claims 1 to 3, and uniformly mixing to obtain mixed bacterial powder;
(2) sterilizing the cassava residue and the peanut straw powder, and naturally cooling for later use;
(3) uniformly mixing ammonium molybdate, zinc sulfate, borax, potassium fulvate, manganese citrate and the mixed bacteria powder in proportion to obtain a mixture;
(4) and (3) uniformly mixing the mixture obtained in the step (3) with the cassava residue and the peanut straw powder treated in the step (2) in proportion.
5. Use of the composite microbial fertilizer of any one of claims 1 to 3 in 1) to 6) below:
1) the application as a base fertilizer for crops;
2) the application in preparing crop base fertilizer;
3) the application as the top dressing of crops;
4) the application in preparing crop base fertilizer;
5) the application in preparing the fertilizer for improving the crop yield;
6) the application in crop planting.
6. Use according to claim 5, characterized in that: the crops are grain and oil crops, including peanut, corn, rape and rice.
7. Use of the composite microbial fertilizer of any one of claims 1-3 for controlling aflatoxin infestation or/and toxin contamination of crops.
8. Use according to claim 7, characterized in that: the crops are grain and oil crops, including peanuts, corns, rapes and rice;
the prevention and control refers to the prevention and control of the aflatoxin infection or/and the toxin pollution in the growing period of crops.
9. The method for using the compound microbial fertilizer as claimed in any one of claims 1 to 3, wherein the compound microbial fertilizer is used as a base fertilizer for crops or/and is used as a top dressing for crops.
10. Use according to claim 9, characterized in that: the crops are grain and oil crops, including peanuts, corns, rapes and rice;
the base fertilizer is used in an amount of 20-40 kg per mu, and the additional fertilizer is used in an amount of 10-20 kg per mu.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113980854A (en) * | 2021-11-15 | 2022-01-28 | 中国农业科学院油料作物研究所 | Microbial agent for promoting leguminous crops to increase root nodule quantity and root nodule nitrogenase activity and application thereof |
| CN114196572A (en) * | 2021-11-15 | 2022-03-18 | 中国农业科学院油料作物研究所 | Microbial agent with aflatoxin, toxigenic bacteria prevention and control and crop yield increase promotion and application thereof |
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| CN105802859A (en) * | 2016-05-03 | 2016-07-27 | 唐山市畜牧水产品质量监测中心 | Trichoderoma koningii TG-102 and application of trichoderoma koningii TG-102 to prevention and control of corn aflatoxin pollution |
| CN106431636A (en) * | 2016-09-06 | 2017-02-22 | 山东农业大学 | Compound microbial fertilizer for winter wheat and preparation method thereof |
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| CN105586300A (en) * | 2016-03-18 | 2016-05-18 | 中国农业科学院油料作物研究所 | Enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus |
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| CN106431636A (en) * | 2016-09-06 | 2017-02-22 | 山东农业大学 | Compound microbial fertilizer for winter wheat and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113980854A (en) * | 2021-11-15 | 2022-01-28 | 中国农业科学院油料作物研究所 | Microbial agent for promoting leguminous crops to increase root nodule quantity and root nodule nitrogenase activity and application thereof |
| CN114196572A (en) * | 2021-11-15 | 2022-03-18 | 中国农业科学院油料作物研究所 | Microbial agent with aflatoxin, toxigenic bacteria prevention and control and crop yield increase promotion and application thereof |
| WO2023083372A1 (en) * | 2021-11-15 | 2023-05-19 | 中国农业科学院油料作物研究所 | Microbial agent with functions of preventing and controlling aflatoxin and toxin-producing bacteria thereof and promoting yield increase of crops and application thereof |
| CN114196572B (en) * | 2021-11-15 | 2023-09-26 | 中国农业科学院油料作物研究所 | Microbial agent with aflatoxin and toxigenic bacteria prevention and control and crop yield increase promotion functions and application thereof |
| CN113980854B (en) * | 2021-11-15 | 2023-11-03 | 中国农业科学院油料作物研究所 | Microbial agent for promoting leguminous crops to increase root nodule number and root nodule nitrogen fixation enzyme activity and application thereof |
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