CN112999422A - 一种骨修复材料的制备方法 - Google Patents

一种骨修复材料的制备方法 Download PDF

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CN112999422A
CN112999422A CN202110306059.3A CN202110306059A CN112999422A CN 112999422 A CN112999422 A CN 112999422A CN 202110306059 A CN202110306059 A CN 202110306059A CN 112999422 A CN112999422 A CN 112999422A
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bone
collagen
mixture
particles
soaking
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仇志烨
刘洋
李洪景
崔孟龙
宋天喜
朱艳泽
胡艳丽
李良才
朱金亮
何志敏
崔云
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Weifang Aojing Medical Research Co ltd
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Weifang Aojing Medical Research Co ltd
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Priority to PCT/CN2021/130480 priority patent/WO2022199051A1/zh
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Abstract

本发明公开了一种骨修复材料的制备方法，包括如下步骤：步骤1、取新鲜牛骨分割后成小块进行脱脂、去抗原处理得到牛骨颗粒；步骤2、将步骤1中的牛骨颗粒浸泡到骨生长因子‑胶原混合物中浸泡后取出后冻干；步骤3、取步骤1中的牛骨颗粒粉碎煅烧后粉碎得到牛骨粉；步骤4、取步骤1中的牛骨颗粒、步骤3中得到的牛骨粉跟胶原、纳米银混合后放入成型模具中冻干成型得到支架材料；步骤5、将步骤4中得到的支架材料交联改性。本发明以牛骨颗粒作为骨修复材料的骨架，起到增加强度的作用，牛骨粉跟胶原的混合物作为填充，再通过交联改性，具有较高的韧性，牛骨颗粒中可缓释骨生长因子，起到促进骨组织再生和重建的作用；填充物中的纳米银具有杀菌的作用。

Description

一种骨修复材料的制备方法
技术领域
本发明涉及骨损伤修复领域，具体的说涉及一种骨修复材料的制备方法。
背景技术
骨科治疗中常常遇到骨损伤的问题。出现骨损伤的原因有很多，运动、事故等造成的骨折会造成骨损伤，治疗骨肿瘤、骨病等过程中也有可能造成骨损伤。当出现骨损伤时就需要在骨组织缺损的地方填充骨修复材料，这样不仅方便对出现骨损伤的部位进行固定，而且有利于骨组织的重新生长。
随着医疗水平的不断发展，骨损伤修复经历了采用自体骨修复材料、采用异体骨修复材料、采用异种骨修复材料、采用人工合成材料等不同阶段。
自体骨修复材料是从患者自身其他的骨组织上取下一部分用作缺损部位的修复，采用自体骨修复材料的缺点是来源不足、容易引起并发症。异体骨修复材料是利用医疗捐献的其他个体的骨组织用作骨缺损部位的修复，采用异体骨修复材料的缺点是容易感染病毒。异种骨修复材料是取用牛骨、猪骨的一部分用于骨缺损部位的修复，直接采用异种骨修复材料的缺点是容易引发排异反应。
人工合成材料是利用人工合成或天然存在的高分子化合物或无机物制作成块，用作骨缺损部分的修复。由于人工合成材料具有取材广泛、没有感染病毒的隐患、不易出现排异反应等优点，近年来得以快速发展。然而现有的人工合成材料的也有其不足之处：1）强度和韧性不够，应用范围受限；2）人工合成材料中缺少生长因子，骨组织再生和重建的速度较慢。
发明内容
本发明的目的是针对以上问题提供一种强度和韧性较高并有促进骨组织再生和重建作用的骨修复材料的制备方法。
为达到上述目的，本发明包括如下步骤：步骤1、取新鲜牛骨分割后成小块进行脱脂、去抗原处理得到牛骨颗粒；步骤2、将步骤1中的牛骨颗粒浸泡到骨生长因子-胶原混合物中浸泡后取出后冻干；步骤3、取步骤1中的牛骨颗粒粉碎煅烧后粉碎得到牛骨粉；步骤4、取步骤1中的牛骨颗粒、步骤3中得到的牛骨粉跟胶原、纳米银混合后放入成型模具中冻干成型得到支架材料；步骤5、将步骤4中得到的支架材料交联改性。
所述步骤1包括：取新鲜牛骨分割成10×10×10mm的颗粒，温水洗净后放入丙酮液中浸泡12小时，取出待丙酮挥发后用乙醚浸泡5小时实现脱脂，脱脂后置于过氧化氢浓度为8-10％的双氧水中保持5小时实现去抗原。
所述步骤2中的骨生长因子为胰岛素样生长因子-1、骨形态发生蛋白-2或成纤维细胞生长因子-2。
所述步骤3中牛骨粉为80～120目的颗粒。
所述步骤4中的胶原为I 型胶原、II 型胶原或IM 型胶原。
所述步骤5包括：将步骤4中的支架材料用0.3%的戊二醛溶液浸泡5小时，用去离子水冲洗3次，用0.3%的赖氨酸溶液浸泡3小时，用去离子水冲洗3次。
综上所述，本发明的有益效果在于：牛骨颗粒作为骨修复材料的骨架，起到增加强度的作用，同时牛骨颗粒中含有骨生长因子，骨生长因子可缓慢释放，起到促进骨组织再生和重建的作用；牛骨粉跟胶原的混合物作为填充，再通过交联改性，使制作成的骨修复材料具有较高的韧性，制作过程中混合了纳米银，纳米银是将粒径做到纳米级的金属银单质，使骨修复材料具有杀菌的作用。
具体实施方式
实施例1
步骤1、取新鲜牛骨分割后成小块进行脱脂、去抗原处理得到牛骨颗粒。该步骤具体包括：取新鲜牛骨分割成10×10×10mm的颗粒，温水洗净后放入丙酮液中浸泡12小时，取出待丙酮挥发后用乙醚浸泡5小时实现脱脂，脱脂后置于过氧化氢浓度为8％的双氧水中保持5小时实现去抗原。
步骤2、将步骤1中的牛骨颗粒浸泡到骨生长因子-胶原混合物中浸泡后取出后冻干；本实施例中的骨生长因子为胰岛素样生长因子-1。
步骤3、取步骤1中的牛骨颗粒粉碎煅烧后粉碎得到牛骨粉；牛骨粉为100目的颗粒。
步骤4、取步骤1中的牛骨颗粒、步骤3中得到的牛骨粉跟胶原、纳米银混合后放入成型模具中冻干成型得到支架材料；本实施例中的胶原为I 型胶原。
步骤5、将步骤4中得到的支架材料交联改性。该步骤具体包括：将步骤4中的支架材料用0.3%的戊二醛溶液浸泡5小时，用去离子水冲洗3次，用0.3%的赖氨酸溶液浸泡3小时，用去离子水冲洗3次。
实施例2
步骤1、取新鲜牛骨分割后成小块进行脱脂、去抗原处理得到牛骨颗粒。该步骤具体包括：取新鲜牛骨分割成10×10×10mm的颗粒，温水洗净后放入丙酮液中浸泡12小时，取出待丙酮挥发后用乙醚浸泡5小时实现脱脂，脱脂后置于过氧化氢浓度为9％的双氧水中保持5小时实现去抗原。
步骤2、将步骤1中的牛骨颗粒浸泡到骨生长因子-胶原混合物中浸泡后取出后冻干；本实施例中的骨生长因子为骨形态发生蛋白-2。
步骤3、取步骤1中的牛骨颗粒粉碎煅烧后粉碎得到牛骨粉；牛骨粉为120目的颗粒。
步骤4、取步骤1中的牛骨颗粒、步骤3中得到的牛骨粉跟胶原、纳米银混合后放入成型模具中冻干成型得到支架材料；本实施例中的胶原为II 型胶原。
步骤5、将步骤4中得到的支架材料交联改性。该步骤具体包括：将步骤4中的支架材料用0.3%的戊二醛溶液浸泡5小时，用去离子水冲洗3次，用0.3%的赖氨酸溶液浸泡3小时，用去离子水冲洗3次。
实施例3
步骤1、取新鲜牛骨分割后成小块进行脱脂、去抗原处理得到牛骨颗粒。该步骤具体包括：取新鲜牛骨分割成10×10×10mm的颗粒，温水洗净后放入丙酮液中浸泡12小时，取出待丙酮挥发后用乙醚浸泡5小时实现脱脂，脱脂后置于过氧化氢浓度为10％的双氧水中保持5小时实现去抗原。
步骤2、将步骤1中的牛骨颗粒浸泡到骨生长因子-胶原混合物中浸泡后取出后冻干；本实施例中的骨生长因子为成纤维细胞生长因子-2。
步骤3、取步骤1中的牛骨颗粒粉碎煅烧后粉碎得到牛骨粉；牛骨粉为80目的颗粒。
步骤4、取步骤1中的牛骨颗粒、步骤3中得到的牛骨粉跟胶原、纳米银混合后放入成型模具中冻干成型得到支架材料；本实施例中的胶原为IM 型胶原。
步骤5、将步骤4中得到的支架材料交联改性。该步骤具体包括：将步骤4中的支架材料用0.3%的戊二醛溶液浸泡5小时，用去离子水冲洗3次，用0.3%的赖氨酸溶液浸泡3小时，用去离子水冲洗3次。
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明技术原理的前提下，还可以做出若干改进和替换，这些改进和替换也应视为本发明的保护范围。

Claims (6)

1.一种骨修复材料的制备方法，其特征在于,该骨修复材料的制备方法包括如下步骤：
步骤1、取新鲜牛骨分割后成小块进行脱脂、去抗原处理得到牛骨颗粒；
步骤2、将步骤1中的牛骨颗粒浸泡到骨生长因子-胶原混合物中浸泡后取出后冻干；
步骤3、取步骤1中的牛骨颗粒粉碎煅烧后粉碎得到牛骨粉；
步骤4、取步骤1中的牛骨颗粒、步骤3中得到的牛骨粉跟胶原、纳米银混合后放入成型模具中冻干成型得到支架材料；
步骤5、将步骤4中得到的支架材料交联改性。
2.根据权利要求1所述的骨修复材料的制备方法，其特征在于，所述步骤1包括：取新鲜牛骨分割成10×10×10mm的颗粒，温水洗净后放入丙酮液中浸泡12小时，取出待丙酮挥发后用乙醚浸泡5小时实现脱脂，脱脂后置于过氧化氢浓度为8-10％的双氧水中保持5小时实现去抗原。
3.根据权利要求1所述的骨修复材料的制备方法，其特征在于，所述步骤2中的骨生长因子为胰岛素样生长因子-1、骨形态发生蛋白-2或成纤维细胞生长因子-2。
4.根据权利要求1所述的骨修复材料的制备方法，其特征在于，所述步骤3中牛骨粉为80～120目的颗粒。
5.根据权利要求1所述的骨修复材料的制备方法，其特征在于，所述步骤4中的胶原为I型胶原、II 型胶原或IM 型胶原。
6.根据权利要求1所述的骨修复材料的制备方法，其特征在于，所述步骤5包括：将步骤4中的支架材料用0.3%的戊二醛溶液浸泡5小时，用去离子水冲洗3次，用0.3%的赖氨酸溶液浸泡3小时，用去离子水冲洗3次。
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113546216A (zh) * 2021-07-14 2021-10-26 陕西巨子生物技术有限公司 胶原蛋白膜微裹骨粉复合材料及其制备方法
WO2022199051A1 (zh) * 2021-03-23 2022-09-29 潍坊奥精医学研究有限公司 一种骨修复材料的制备方法
CN115317670A (zh) * 2022-08-05 2022-11-11 武汉启思达科技发展有限公司 一种可塑型煅烧骨修复材料及其制备方法
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Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4314380A (en) * 1980-09-26 1982-02-09 Koken Co., Ltd. Artificial bone
GB8819755D0 (en) * 1988-08-19 1988-09-21 Geistlich Soehne Ag Chemical compound
AU7454694A (en) * 1993-07-21 1995-02-20 Faissol Pinto, Olympio Process for preparing an osseous ceramic material and bone regenerating and growth promoting composition
US20030180263A1 (en) * 2002-02-21 2003-09-25 Peter Geistlich Resorbable extracellular matrix for reconstruction of bone
DK177997B1 (da) * 2000-07-19 2015-02-23 Ed Geistlich Söhne Ag Für Chemische Ind Knoglemateriale og collagenkombination til opheling af beskadigede led
CN1200740C (zh) * 2003-05-28 2005-05-11 王庆贤 仿生骨的制备方法
CN101954122A (zh) * 2010-09-13 2011-01-26 陕西瑞盛生物科技有限公司 具有预塑性天然骨修复材料的制备方法
RU2640932C2 (ru) * 2016-07-04 2018-01-12 Федеральное государственное бюджетное учреждение "Новосибирский научно-исследовательский институт травматологии и ортопедии им. Я.Л. Цивьяна" Министерства здравоохранения Российской Федерации (ФГБУ "ННИИТО им. Я.Л. Цивьяна" Минздрава России) Способ получения костно-пластического материала
CN112999422A (zh) * 2021-03-23 2021-06-22 潍坊奥精医学研究有限公司 一种骨修复材料的制备方法

Cited By (6)

* Cited by examiner, † Cited by third party
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WO2022199051A1 (zh) * 2021-03-23 2022-09-29 潍坊奥精医学研究有限公司 一种骨修复材料的制备方法
CN113546216A (zh) * 2021-07-14 2021-10-26 陕西巨子生物技术有限公司 胶原蛋白膜微裹骨粉复合材料及其制备方法
CN115317670A (zh) * 2022-08-05 2022-11-11 武汉启思达科技发展有限公司 一种可塑型煅烧骨修复材料及其制备方法
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