CN112999140B

CN112999140B - Composition with anti-pollution and anti-damage effects and skin care product thereof

Info

Publication number: CN112999140B
Application number: CN202110218858.5A
Authority: CN
Inventors: 刘菲; 王倩; 杨素珍; 韩婷婷; 郭凤娇; 陈玉荣
Original assignee: Shandong Furida Biological Co ltd
Current assignee: Shandong Furida Biological Co ltd
Priority date: 2021-02-26
Filing date: 2021-02-26
Publication date: 2022-05-13
Anticipated expiration: 2041-02-26
Also published as: CN112999140A

Abstract

The invention provides a composition with an anti-pollution and anti-damage effect and a skin care product thereof. The composition of the present invention comprises the following components: arginine/lysine polypeptide, artemia extract, and hydrolyzed rice protein. The composition plays a synergistic role in resisting pollution injury by regulating and controlling the interaction between mitochondria and ubiquitin-proteasome, and can resist the injury of skin caused by pollutants by adding the composition into skin care products, such as relieving inflammatory reaction of the skin, removing damaged proteins and mitochondria in time, reducing the attack of active oxygen free radicals to cells and the like, thereby playing a role in protecting the skin.

Description

Composition with anti-pollution and anti-damage effects and skin care product thereof

Technical Field

The invention belongs to the field of cosmetics, and relates to a composition with an anti-pollution and anti-damage effect and a skin care product thereof.

Background

The constituents of PM2.5 are very complex and mainly classified into five types: water-soluble salts (sulfate, sodium salt, nitrate, ammonium salt, etc.), strongly toxic heavy metal elements (lead, copper, zinc, arsenic, etc.), organic substances (alkanes, aromatics, polycyclic aromatics, alkenes, quinones, etc.), low-toxic or nontoxic metal elements (sodium, magnesium, aluminum, iron, etc.), and microorganisms (bacteria, mold, actinomycetes, etc.), which are extremely harmful to the skin.

PM2.5, when contacted with the skin, causes toxic damage to keratinocytes. Firstly, keratinocytes release inflammatory factors IL-1 alpha and TNF-alpha, and activate NF-kB signal path to produce a large amount of inflammatory factors, such as IL-8 and COX₂、PEG₂VCAM-1, and the like, thereby causing immune cell recruitment and migration, and vascular reactions such as vasodilatation and permeability change, and finally causing acute phase reactions of red, swollen, painful and itchy skin inflammation; with the continuous accumulation of inflammatory factors, when the oxidative toxicity of air pollutants exceeds the capacity of the cell detoxification system, the oxidative toxicity is uncontrolledThe reactive oxygen species ROS can attack cellular lipid and protein, so that lipid peroxidation and protein oxidation are caused, and even mitochondrial dysfunction is caused, calcium ion disorder is caused, and abnormal apoptosis of cells is caused; the dysfunctional mitochondria can continuously generate a large amount of free radicals to induce the uninterrupted release of inflammatory factors to cause chronic inflammatory reaction, and if the damaged mitochondria can not be removed in time, the damaged mitochondria can not only cause serious damage to skin for a long time, such as sensitive, abnormal desquamation and premature aging, but also can cause mitochondrial dysfunction diseases such as neurodegenerative diseases, coronary heart diseases, diabetes and the like. In addition, since the inflammatory reaction reduces or partially removes the thiol groups in the skin, the reduction of thiol groups increases tyrosinase activity, possibly causing skin pigmentation; and polycyclic aromatic hydrocarbon carried by PM2.5 can activate aromatic hydrocarbon receptor (AhR), and further induce POMC to promote synthesis of melanin, and form persistent color precipitate.

At present, raw materials for resisting pollution injury continuously appear, but most of the raw materials only aim at eliminating acute inflammation, and the raw materials are difficult to play a role in injury caused by chronic inflammation.

Mitochondria are the "energy factory" of the human body, the main site for cellular synthesis of ATP, which is the direct energy source for cells and vital activities, providing more than 90% of the energy required for cellular activities. The healthy and efficient mitochondria can provide enough ATP for normal metabolism of the mitochondria, and can generate free radicals generated by antioxidants and normal life activities, so that the free radicals in the body can be maintained at a proper normal level, which is beneficial to the organism. However, when mitochondria are damaged, insufficient ATP production is caused, and excessive free radicals are generated to cause cell damage. If damaged mitochondria are not cleared in time, skin problems such as inflammation, pigmentation, wrinkles, etc. are induced.

The ubiquitin-proteasome system is the main pathway for about 80% of protein degradation in human body, most functional proteins need to be degraded under specific spatiotemporal conditions after exerting their effects, the misfolded proteins and the proteins in food need to be degraded by proteasome, and the degradation process of damaged mitochondria and related proteins also needs to involve ubiquitin-proteasome, which needs ATP energy supply. When the activity of proteasome is reduced due to aging of the body itself or the influence of external environment, damaged proteins and damaged mitochondria are accumulated in the body continuously, thereby inducing skin problems such as inflammation, pigmentation, wrinkles, etc., and when the accumulation is excessive, the activity of ubiquitin-proteasome is inhibited in turn, thereby forming a vicious circle.

Therefore, the mitochondria and the ubiquitin-proteasome have a relationship of mutual influence and mutual restriction, but at present, no patent/literature report for achieving the effect of protecting the skin by utilizing mutual regulation between the mitochondria and the ubiquitin-proteasome is found.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a composition with an anti-pollution injury effect, which comprises arginine/lysine polypeptide, artemia extract and hydrolyzed rice protein, has a synergistic effect on anti-pollution injury by regulating and controlling the interaction between mitochondria and ubiquitin-proteasome, can resist the injury of pollutants to skin by adding the composition into a skin care product, and has the effects of relieving skin inflammation reaction, timely removing damaged protein and mitochondria, reducing the attack of active oxygen free radicals to cells and the like, thereby having the effect of protecting the skin.

In order to achieve the purpose, the invention provides the following technical scheme: a composition having anti-contaminant damage properties comprising the following components: arginine/lysine polypeptide, artemia extract and hydrolyzed rice protein, wherein the mass ratio of the three components in the anti-pollution damage skin care product is as follows: arginine/lysine polypeptide 0.000001-0.001%; 0.00025-0.25% of artemia salina extract; 0.00005-0.05% of hydrolyzed rice protein. The above components are all calculated on dry matter.

Preferably, the three components are contained in the anti-pollution damage skin care product in a mass ratio of: arginine/lysine polypeptide 0.00001-0.0001%; 0.0025-0.025% of artemia salina extract; 0.0005-0.005% of hydrolyzed rice protein.

Among them, a known commercial product of arginine/lysine polypeptide is Hangzhou coma science and technology, Inc

(trade name) series raw materials with the molecular weight of 3000-5500 and the polypeptide sequence length of 20-40 amino acids are obtained by an artificial synthesis method. Researches prove that the polypeptide can specifically act on mitochondria, protect and activate the mitochondria, and improve the ATP synthesis of the organism, thereby enabling various vital activities of cells.

The artemia extract is obtained by adding artemia cysts into distilled water for rehydration, grinding, centrifuging, filtering and sterilizing to obtain an aqueous solution (see patent CN 106132396B), wherein the content of diguanosine tetraphosphate (GP4G) is 145-195mg/kg, and the aqueous solution is subjected to freeze drying to obtain the artemia extract. Artemia, a unique polar plankton, is rich in GP4G, which is a precursor of ATP and can be directly converted into ATP in cells to energize various vital activities of the cells, thus enabling the artemia to resist extreme environmental stress.

Hydrolyzed rice protein is prepared by hydrolyzing rice with papain, removing macromolecular polypeptide to obtain hydrolyzed rice protein aqueous solution (see patent CN 102439027B) containing peptides with molecular weight less than 6kDa, and having protein content of 3.5-5.5g/L, and freeze drying the aqueous solution to obtain hydrolyzed rice protein containing peptides with molecular weight less than 6kDa and rich in bioactive peptides of 3-5 amino acids including at least one aspartic acid residue, one cysteine residue and one arginine residue, and having proteasome activating effect.

After extensive literature search and experiments, the inventor of the application finds that the composition of the invention can play a role in both the acute phase and the chronic phase of inflammation caused by PM 2.5. In the acute inflammation stage, arginine/lysine polypeptide acts on mitochondria to stimulate the activity of the mitochondria, improve the synthesis of ATP of the organism per se and improve the content of endogenous ATP, and artemia extract can be directly converted into ATP to improve the content of exogenous ATP, so that the arginine/lysine polypeptide and the artemia extract can energize cells of the organism together, accelerate the metabolism of the cells, stimulate the resistance of the cells to external stimulation and reduce the expression of inflammatory factors.

In a chronic inflammation stage, inflammatory factors, free radicals and the like attack mitochondria to cause mitochondrial damage and slow or abnormal cell metabolism, at the moment, arginine/lysine polypeptide can protect and activate mitochondria in a targeted manner, and the arginine/lysine polypeptide and artemia extract together improve the ATP content and provide required ATP for ubiquitin-proteasome activation, so that the degradation and the elimination of damaged proteins (such as carbonylation proteins) are accelerated, the accumulation of the damaged proteins is reduced, and the secondary damage to cells is reduced; meanwhile, the hydrolyzed rice protein can activate ubiquitin-proteasomes, and the ubiquitin-proteasomes can assist the degradation of mitochondria for seriously damaged mitochondria to control the quality of the mitochondria, thereby relieving cell damage caused by excessive accumulation of the damaged mitochondria. The three raw materials have synergistic effect and obvious effect in the process of relieving chronic inflammation.

The anti-pollution damage composition can be added into skin care products such as facial masks, essence, emulsion, cream and the like. The skin care product containing the composition can resist the damage of pollutants to the skin, such as relieving skin inflammation reaction, timely eliminating damaged protein and mitochondria, reducing the attack of active oxygen free radicals to cells, and the like, thereby playing the role of protecting the skin.

Further, the invention provides a skin care product with anti-pollution and damage effects, which comprises the following 3 components in parts by weight: arginine/lysine polypeptide 0.000001-0.001%, artemia extract 0.00025-0.25%, hydrolyzed rice protein 0.00005-0.05%, humectant 0.1-10%, emulsifier 0.001-5%, skin conditioner 0.1-15%, emollient 0.01-30%, thickener 0.001-5%, antiseptic 0.001-2%, other skin care product auxiliary agent 0.001-5%, and water to make up 100%.

Wherein the humectant, emulsifier, skin conditioning agent (including at least one of oil-soluble skin conditioning agent and water-soluble skin conditioning agent), emollient, thickener, and antiseptic (including at least one of water-soluble antiseptic and oil-soluble antiseptic) are all prepared from materials commonly used in commercially available skin care products. Other skin care product adjuvants include one or more of metal ion chelating agent, sunscreen agent, aromatic agent, and pH regulator.

The preparation method of the skin care product comprises the following steps:

(1) adding emulsifier, emollient, sunscreen agent (if contained in the formulation), oil-soluble skin conditioner (if contained in the formulation) and oil-soluble antiseptic (if contained in the formulation) into oil phase tank, heating to 75-80 deg.C, stirring under heat preservation to dissolve completely to obtain phase A;

(2) adding humectant, metal ion chelating agent (if contained in the formula), thickener and water soluble antiseptic (if contained in the formula) into water, heating to 80-85 deg.C, stirring under heat preservation to dissolve completely to obtain phase B;

(3) slowly adding phase A into phase B, maintaining the temperature at 75-80 deg.C, emulsifying and homogenizing for 10-15min to obtain uniform emulsion, adding pH regulator (if the formula contains pH regulator), stirring, and cooling with cooling water;

(4) cooling to 40-45 deg.C, adding composition (arginine/lysine polypeptide, artemia salina extract, hydrolyzed rice protein), water soluble skin conditioner (if formula contains), and aromatic (if formula contains), and stirring to obtain skin care product with anti-pollution and anti-injury effects.

Compared with the prior art, the invention has the following beneficial effects:

(1) the composition with pollution and damage resistance and the skin care product thereof provided by the invention are characterized in that arginine/lysine polypeptide with the functions of protecting and activating mitochondria, artemia extract for supplying energy to the mitochondria and hydrolyzed rice protein with a proteasome activation function are combined to activate the self defense capability of cells, so that the skin is better helped to resist external damage, the inflammatory reaction is relieved, and the self repair capability of the skin is accelerated.

(2) When the arginine/lysine polypeptide, the artemia extract and the hydrolyzed rice protein are used in combination, the arginine/lysine polypeptide and the artemia extract can provide ATP required by the function of the hydrolyzed rice protein, the hydrolyzed rice protein can activate proteasomes to degrade damaged mitochondria, so that the arginine/lysine polypeptide and the artemia extract can better exert an energizing effect, and the arginine/lysine polypeptide, the artemia extract and the hydrolyzed rice protein are synergistic with each other, so that the skin damage caused by environmental pollution is effectively resisted, the chronic inflammation caused by PM2.5 is improved, and the skin barrier is repaired.

Drawings

FIG. 1 is a graph showing the results of a test of the ability of the compositions of examples 1 to 2 and comparative examples 1 to 3 to repair HaCaT cell damage;

FIG. 2 is a graph showing the results of ROS scavenging experiments with compositions of examples 1-2 and comparative examples 1-3;

FIG. 3 is a graph showing the effect of the compositions of examples 1 to 2 and comparative examples 1 to 3 on IL-1. alpha. expression;

FIG. 4 is a graph showing the effect of the compositions of examples 1 to 2 and comparative examples 1 to 3 on the amount of carbonylated protein;

FIG. 5 is a graph showing the effect of example 2 on TEWL;

FIG. 6 is a graph showing the results of example 2 on the skin color;

wherein, # denotes p <0.05, # denotes p <0.01, # denotes p <0.001 in FIGS. 1-4, compared to the blank group; in comparison to the PM2.5 model group, denotes p <0.05, denotes p <0.01, denotes p < 0.001;

in fig. 5-6, p <0.05, p <0.01, and p <0.001, as compared to the placebo group.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples. Examples of arginine/lysine polypeptides used are Hangzhou coma science and technology, Inc

(trade name) series of raw materials, which were aqueous solutions of arginine/lysine polypeptide, the content of which was 10 ppm. In the examples, an aqueous solution of artemia salina extract was used, with a content of 0.5%. In the examples, a 0.5% content mixed solution of hydrolyzed rice protein glycerin (30%) and water (69.5%) was used. The above contents are mass ratios.

The humectant used in the present invention includes at least one of glycerin, propylene glycol, butylene glycol, dipropylene glycol, hyaluronic acid, polyglutamic acid, trehalose, betaine, pullulanase, and acetyl chitosamine. The emulsifier comprises at least one of PEG-100 stearate, glyceryl stearate, cetearyl glucoside, cetearyl alcohol, potassium cetyl phosphate, cetearyl olive oleate, sorbitan olive oleate, C14-22 alcohol, C12-20 alkyl glucoside, hydrogenated lecithin, polyglycerol-10 laurate, polyglycerol-6 distearate, jojoba esters, polyglycerol-3 beeswax, cetyl alcohol, PEG-60 hydrogenated castor oil, polysorbate-80, polysorbate-20, polyglycerol-6 stearate, polyglycerol-6 behenate, inulin lauryl carbamate, and sucrose laurate. The skin conditioning agent comprises at least one of oil-soluble skin conditioning agent such as ubiquinone, bisabolol, ginger root extract, tocopherol acetate and water-soluble skin conditioning agent such as beta-glucan, nicotinamide, ascorbic acid, dipotassium glycyrrhizinate, panthenol, plant extract, and polypeptide. The emollient comprises at least one of cetostearyl alcohol, shea butter, dioctyl carbonate, isononyl isononanoate, caprylic/capric triglyceride, jojoba seed oil, squalane, dimethicone, cyclopentadimethicone, cyclohexasiloxane, triglycerides of C10-18 fatty acids, meadowfoam seed oil, and dimethicone crosspolymer. The thickening agent comprises at least one of polyacrylate-13, ammonium acryloyldimethyltaurate/VP copolymer, sodium polyacrylate, hydroxyethyl acrylate/sodium acryloyldimethyltaurate copolymer, acrylic acid (ester)/vinyl isodecanoate cross-linked polymer, polyacrylic acid, sodium acrylate/sodium acryloyldimethyltaurate copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, ammonium acryloyldimethyltaurate/behenyl alcohol polyether-25 methacrylate cross-linked polymer, carbomer, hydroxyethyl cellulose, hydroxypropyl methyl cellulose and xanthan gum. The antiseptic includes at least one of water soluble antiseptic such as methyl hydroxybenzoate, chlorphenesin, phenoxyethanol, p-hydroxyacetophenone, caprylyl hydroxamic acid, sodium benzoate, potassium sorbate and oil soluble antiseptic propyl hydroxybenzoate.

Examples 1 to 2 provide skin care products containing the composition having an anti-contaminant damage effect, and the raw material formulation thereof is shown in table 1 below, and for comparison, table 1 also provides comparative examples 1 to 3. Comparative examples 1-3 arginine/lysine polypeptide, artemia extract and hydrolyzed rice protein were omitted from comparative example 2, respectively.

TABLE 1 raw material formulation of anti-contaminant cosmetic composition for examples 1 to 2 and comparative examples 1 to 3

The preparation method of the skin care product comprises the following steps:

(1) sequentially adding the components of the phase A into an oil phase tank, heating to 75-80 ℃, preserving heat and stirring until the components are completely dissolved to prepare a phase A;

(2) dispersing carbomer with glycerol and butanediol, adding into water, heating to 80-85 deg.C, adding methylparaben and chlorphenesin, stirring under heat preservation to dissolve completely, and making into phase B;

(3) slowly adding phase A into phase B, maintaining the temperature at 75-80 deg.C, emulsifying and homogenizing for 10-15min until uniform emulsion is formed, adding triethanolamine, stirring, and cooling with cooling water;

(4) and when the temperature is reduced to 40-45 ℃, adding the C phase components, and uniformly stirring to obtain the skin care product with the pollution and damage resistance function.

Performance testing

The compositions of examples 1-2 and comparative examples 1-3 were tested for their performance as follows:

preparation of experimental materials:

1. the skin care products prepared in comparative examples 1 to 3 and examples 1 to 2 were diluted to 20% (g/g, mass fraction) using serum-free DMEM medium, centrifuged to obtain a supernatant, and filtered with a 0.22 μm filter to prepare mother solutions of the samples of comparative examples 1 to 3/examples 1 to 2 for the following cell experiments.

2. Collecting PM2.5 and preparing dry powder: and (3) collecting PM2.5 in the air by using a large-flow collector. Sampling is carried out for 24h every day, and the sampling time duration is 1 month in total. After sampling is finished, PM2.5 is collectedThe filter membrane is cut into 1 × l cm²Soaking in ultrapure water, ultrasonically oscillating at low temperature for 30min, filtering with six layers of gauze to obtain oscillation liquid, repeating the soaking, oscillating and filtering steps with filter membrane for 3 times, and mixing filtrates. The filtrate was vacuum frozen and dried to prepare a dry powder, which was stored in a refrigerator at 4 ℃ for the following cell experiments.

First, composition for testing repair ability of PM2.5 induced HaCaT cell damage

1. The experimental method comprises the following steps: the experiment is provided with a blank group (no treatment), a model group (50 mu g/ml PM2.5 dry powder), and a sample group (50 mu g/ml PM2.5 dry powder + 10% comparative examples 1-3/examples 1-2), wherein 3 parallel cells are arranged in each group, HaCaT cells with good growth state are selected, corresponding treatment is carried out according to the group, and after 24h of culture, the cell survival rate is detected by an MTT method.

2. The experimental results are as follows: as shown in fig. 1, the cell viability of the PM2.5 model group is only about (50.22 ± 1.81)% of that of the blank group, which indicates that PM2.5 has serious damage to cells and successful modeling. In comparative examples 1-3, the cell viability rates were increased by (11.35 ± 0.77)%, (17.87 ± 1.31)%, and (13.32 ± 1.56)%, respectively, compared to the model group; the cell viability rates of the groups in examples 1-2 are significantly higher than those of the groups in comparative examples 1-3, and compared with the model group, the cell viability rates are respectively improved by (30.67 +/-0.80)%, (37.61 +/-0.86)%, which shows that the composition in examples 1-2 has a good repairing function on HaCaT cell injury induced by PM2.5, and can resist skin injury caused by PM2.5 to a certain extent.

Second, the Effect of the composition on PM 2.5-induced Reactive Oxygen Species (ROS) content in HaCaT cells

1. The experimental method comprises the following steps: the experiment is provided with a blank group (no treatment), a model group (50 mu g/ml PM2.5 dry powder), and a sample group (50 mu g/ml PM2.5 dry powder + 10% comparative examples 1-3/examples 1-2), wherein each group is provided with 3 parallels, HaCaT cells with good growth state are selected, the cells are correspondingly treated according to the group, after 24 hours of culture, after the operation according to the instruction of an active oxygen detection kit (Shanghai Biyuntan biology), DCFH-DA is added to continue to be cultured for 30 minutes, excitation is carried out by a multifunctional microplate reader at 488nm, and the fluorescence intensity of each hole of the culture plate is detected under the emission wavelength of 525 nm.

2. The experimental results are as follows: as shown in fig. 2, in which ROS increased significantly in HaCaT cells after PM2.5 treatment, the increase was about (1.94 ± 0.09) fold compared to the blank group, indicating that PM2.5 causes respiratory chain metabolic disorders, free radical imbalance. After the comparative examples 1 to 3 are added, the content of ROS in cells can be reduced, compared with a model group, the ROS content is respectively reduced by (0.47 +/-0.07), (0.88 +/-0.06) and (0.60 +/-0.05), while the ROS increase caused by PM2.5 can be obviously reduced by examples 1 to 2, and the ROS increase caused by PM2.5 is respectively reduced by about (1.37 +/-0.05) and (1.59 +/-0.04) times compared with the model group, which shows that the composition has certain repair capability on mitochondrial respiratory chain damage and can reduce cell damage caused by ROS.

Third, the effect of the composition on PM 2.5-induced expression of IL-1 alpha of HaCaT cells

1. The experimental method comprises the following steps: setting a blank group (without treatment), a model group (50 mu g/ml PM2.5 dry powder), a sample group (50 mu g/ml PM2.5 dry powder + 10% comparative examples 1-3/examples 1-2 samples) in an experiment, setting 3 parallel cells in each group, selecting HaCaT cells with good growth state, performing corresponding treatment according to the group, culturing for 24h, extracting total RNA in each group of cells by using a Trizol method, and performing reverse transcription to obtain cDNA. And detecting the change of the expression level of the treated IL-1 alpha by using a real-time fluorescent quantitative PCR method. The method is performed according to kit instructions.

2. The experimental results are as follows: after PM2.5 treatment, the IL-1 alpha expression level of HaCaT cells is obviously increased, and the composition can reduce the IL-1 alpha expression level and play a certain role in protecting the HaCaT cells. As shown in FIG. 3, the expression level of IL-1 α in the PM2.5 model group is (7.75. + -. 0.68) times that in the blank group, and the IL-1 α expression levels are significantly reduced compared to the model group and the examples 1 to 2 and comparative examples 1 to 3 are better than the comparative examples in which the expression levels in comparative examples 1 to 3 and examples 1 to 2 are respectively reduced by (2.28. + -. 0.29), (1.98. + -. 0.15), (3.06. + -. 0.12), (4.67. + -. 0.10) and (5.48. + -. 0.32) times. The composition has certain inhibition effect on the induction of IL-1 alpha expression by PM2.5, and can reduce skin inflammation caused by PM2.5 to a certain extent.

Fourth, the influence of the composition on the content of PM 2.5-induced protein carbonylated by HaCaT cells

1. The experimental method comprises the following steps: the experiment is provided with a blank group (no treatment), a model group (50 mu g/ml PM2.5 dry powder), and a sample group (50 mu g/ml PM2.5 dry powder + 10% comparative examples 1-3/examples 1-2), wherein each group is provided with 3 parallels, HaCaT cells with good growth state are selected, the corresponding treatment is carried out according to the group, after 24h of culture, the cells are centrifuged at 3000rmp/min for 20min to remove particles and polymers. The supernatant was collected and assayed according to the manual of human carbonylated Protein (PC) ELISA kit (Shanghai Zheke organism).

2. The experimental results are as follows: as shown in fig. 4, cells were strongly carbonylated under PM2.5 treatment, and carbonylated protein increased by (118.47 ± 2.25)%, compared to the group of cells not treated with PM 2.5. In comparative examples 1 and 2 containing hydrolyzed rice protein, the degree of protein carbonylation was reduced by (39.00 ± 2.96)% and (45.16 ± 1.66)%, respectively, compared to the model group, and in comparative example 3 containing no hydrolyzed rice protein, the degree of protein carbonylation was reduced by only (16.06 ± 2.53)%, indicating that hydrolyzed rice protein plays a critical role in reducing protein carbonylation; the example 1 and the example 2 have better removal effect on the carbonylated protein, compared with the model group, the content of the carbonylated protein is respectively reduced by (56.36 +/-4.80)% and (65.52 +/-4.47)%, so that a synergistic effect is achieved, and the composition has better repair and removal effects on PM 2.5-induced protein damage, reduces the accumulation of oxidative damage and reduces chronic damage to the skin.

Human body experiment

The human body efficacy test is carried out on the specific embodiment 2, and the test is carried out in 12 months in Jinan, wherein the test time is 1 month. Data statistics are from https:// www.aqistudy.cn/historydata/.

1. Volunteer selection and request

30 healthy subjects (15 men and women) are aged 30-45 years, the working environment is outdoor, all the subjects have no history of skin or systemic diseases, the tested parts have no abnormality, and no drug or cosmetic irrelevant to the experiment is smeared during the testing period.

2. Test environment

The test site is constant in temperature and humidity, the ambient temperature is 20-22 ℃, the relative humidity is 40-60%, and the test subject should keep the organism in a stable state before testing. After the face of the subject was washed with clear water at about 35 ℃, the test was started after sitting still for 30min in the test environment.

3. Sample preparation

The test sample was example 2 and the placebo group was identical to the base material of example 2, with the difference that there was no composition of the invention. The medicine is used in the morning and evening every day, and the dosage of the medicine is 0.6-1.0 g/time each time.

4. Test items

(1) The experimental method comprises the following steps: transdermal water loss (TEWL value) detection

Self half-face control, example 2 was randomly distributed with placebo. The amount of transdermal water loss in the skin using the test area and the control area of example 2 and placebo for 14 days and 28 days, respectively, was measured using a skin water loss tester (Tewameter, TM300, Courage and Khazaka, germany), and the change rate was calculated with the pre-test base value of 0. The TEWL value may reflect the skin barrier condition, with a range of greater TEWL values leading to poorer skin barrier function.

The experimental results are as follows: as shown in fig. 5, the TEWL value of the example 2 group decreased significantly (p <0.001), about (11.07 ± 2.62)%, compared to the placebo group 28 days after the test sample was applied to the volunteers, which indicates that the composition and the skin care product thereof have significant repairing effect on the skin barrier, can resist the damage of the skin barrier caused by environmental pollution, and can be used in the repairing cosmetics.

(2) The experimental method comprises the following steps: skin color determination (Lab value)

Self half-face control, example 2 was randomly distributed with placebo. The changes in the L, a, b values of the skin in the test and control areas were measured using a Lab color difference meter (Colorreter CL400, Courage and Khazaka, Germany) 14 and 28 days after application of example 2 to placebo. Wherein the L value represents the brightness, and the larger the L value is, the more the color is biased to white; conversely, the color is biased to black; the saturation a of the color on the green-red axis, a negative value represents green, a positive value represents red, and the larger the a value is, the more red the skin color is; the saturation b of the color on the blue-yellow axis, negative values indicate blue, positive values indicate yellow, and the greater the b value, the more yellow the skin color.

The experimental results are as follows: the results are shown in fig. 6, and the L value significantly increased and the a and b values significantly decreased in 30 volunteers after 28 days using the skin care product of example 2. Compared with the placebo group, the L value is improved by (3.88 +/-0.61)%, the a value is reduced by (17.34 +/-2.71)%, and the b value is reduced by (9.52 +/-2.82)%. The skin care product in the embodiment 2 can remove oxidation garbage induced by PM2.5 in skin and improve skin brightness; reduce skin inflammation and reduce skin redness. The composition and the skin care product thereof have very good effect on resisting the skin damage caused by PM 2.5.

Claims

1. A skin care product with an anti-pollution and anti-damage effect is characterized by comprising the following raw materials in percentage by weight: arginine/lysine polypeptide 0.000001-0.001%, artemia extract 0.00025-0.25%, hydrolyzed rice protein 0.00005-0.05%, humectant 0.1-10%, emulsifier 0.001-5%, skin conditioner 0.1-15%, emollient 0.01-30%, thickener 0.001-5%, antiseptic 0.001-2%, other skin care product auxiliary agent 0.001-5%, and water to make up 100%;

the humectant is at least one of glycerol, propylene glycol, butanediol, dipropylene glycol and hyaluronic acid;

the emulsifier is at least one of PEG-100 stearate, glyceryl stearate, cetearyl glucoside, cetearyl olive oleate, C12-20 alkyl glucoside, polyglycerol-6 distearate, polyglycerol-6 stearate, and polyglycerol-6 behenate;

the skin conditioner is at least one of tocopherol acetate, beta-glucan, nicotinamide, dipotassium glycyrrhizinate and panthenol;

the emollient is at least one of cetostearyl alcohol, shea butter, isononyl isononanoate, caprylic/capric triglyceride, jojoba oil, squalane and polydimethylsiloxane;

the thickening agent is at least one of polyacrylate-13, carbomer and xanthan gum;

the antiseptic is at least one of methyl hydroxybenzoate, chlorphenesin, phenoxyethanol, p-hydroxyacetophenone, caprylyl hydroximic acid and propyl hydroxybenzoate.

2. The anti-contaminant damage skin care product of claim 1, wherein the weight ratio of arginine/lysine polypeptide, artemia extract and hydrolyzed rice protein in the anti-contaminant damage skin care product is: arginine/lysine polypeptide 0.00001-0.0001%; 0.0025-0.025% of artemia salina extract; 0.0005-0.005% of hydrolyzed rice protein.