CN112971001A - Efficient composite mildew inhibitor for feed and preparation method thereof - Google Patents
Efficient composite mildew inhibitor for feed and preparation method thereof Download PDFInfo
- Publication number
- CN112971001A CN112971001A CN201911307153.XA CN201911307153A CN112971001A CN 112971001 A CN112971001 A CN 112971001A CN 201911307153 A CN201911307153 A CN 201911307153A CN 112971001 A CN112971001 A CN 112971001A
- Authority
- CN
- China
- Prior art keywords
- parts
- carrier
- feed
- mildew
- nonionic surfactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3499—Organic compounds containing oxygen with doubly-bound oxygen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/358—Inorganic compounds
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Inorganic Chemistry (AREA)
- Microbiology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an efficient compound mildew preventive for feed and a preparation method thereof, wherein the efficient compound mildew preventive comprises the following components in parts by weight: 30-55 parts of propionic acid, 10-20 parts of ammonium propionate, 4-10 parts of sodium benzoate, 0.5-3 parts of nonionic surfactant and 17-55 parts of carrier. The invention has the advantages of good palatability, good mildew-proof effect and lasting mildew-proof effect. Meanwhile, the invention correspondingly discloses a preparation method of the efficient composite mildew inhibitor for the feed, which comprises the following steps: weighing 30-55 parts of propionic acid, 10-20 parts of ammonium propionate, 4-10 parts of sodium benzoate, 0.5-3 parts of nonionic surfactant and 17-55 parts of carrier according to the weight part ratio; homogenizing, namely uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate and nonionic surfactant, and then homogenizing to obtain homogenized liquid; and mixing, namely uniformly mixing the prepared homogenized treatment solution with the weighed carrier by adopting a full-spray mixing method, wherein the mildew preventive prepared by adopting the method has the advantages of good palatability, good mildew prevention effect and lasting mildew prevention effect.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to an efficient compound mildew preventive for feed and a preparation method thereof.
Background
During the storage, transportation, sale and use of the feed, the feed is easy to mildew due to the change of moisture in the feed and the influence of the environment. Feed mildew and mycotoxin poisoning are serious problems commonly existing in livestock and poultry production worldwide, and the control of feed mildew is a non-negligible link in feed production.
The existing substances used as the mildewcide are mainly divided into an inorganic mildewcide and an organic mildewcide. The inorganic mildew inhibitor is mainly silver-based, copper-based and other substances containing metal elements with sterilization and bacteriostasis properties, and although the mildew inhibitor has good heat resistance and durability, the mildew inhibitor has poor mildew-proof effect. Although the organic mildew preventive has a good mildew preventive effect, the organic mildew preventive is easily decomposed by light (ultraviolet rays) and heat, so that the use of the organic mildew preventive is limited, and the organic mildew preventive is easily ineffective due to improper storage. Meanwhile, the mildew preventive is easy to run off under the action of water and an organic solvent, so that the mildew resistance is poor in persistence. Therefore, it is a problem to be solved by those skilled in the art to develop a highly efficient antifungal agent having an excellent antifungal effect and a long lasting antifungal effect.
In order to solve the problems, in the prior art, different mildewcides are compounded to prepare a compound mildewproof agent so as to improve the mildewproof effect and the mildewproof durability. For example, the Chinese patent application with the application publication number of CN1692725A discloses a solid mildew preventive, which is prepared by mixing monomethyl fumarate and/or dimethyl fumarate with 4-hydroxy-3-methoxybenzaldehyde. But the action of the mildew preventive and the solid feed is difficult to achieve uniform distribution, and mildew-proof dead corners are left;
the Chinese patent with the publication number of CN103875916B discloses a feed additive which is added with pseudolarix, montmorillonite, phellodendron, scutellaria and cortex dictamni, and takes natural Chinese herbal medicine components as main components with the function of mildew prevention, but the phellodendron, the scutellaria and the cortex dictamni are all bitter Chinese medicines, the proportion of the three bitter Chinese herbal medicines is more than 50 percent, and the feed additive has the defect of poor palatability when being used for animal feed.
Therefore, there is a need for further improvement of the existing mildewcide for feed.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the efficient compound mildew preventive for the feed, which has the advantages of good palatability, good mildew prevention effect and lasting mildew prevention effect.
The above object of the present invention is achieved by the following technical solutions:
an efficient compound mildew preventive for feed comprises the following components in parts by weight,
30-55 parts of propionic acid
10-20 parts of ammonium propionate
4-10 parts of sodium benzoate
0.5-3 parts of nonionic surfactant
17-55 parts of a carrier.
By adopting the technical scheme, the propionic acid, the ammonium propionate and the sodium benzoate are compounded to be used as active ingredients of the mildew preventive, so that excellent mildew-proof and bacteriostatic effects can be achieved, the irritation of the propionic acid can be neutralized to a certain degree, and the negative influence of the addition of the mildew preventive on the palatability of the feed is reduced. Meanwhile, the mildew preventive obtained by mixing according to the proportion has a buffering property, and the neutralization effect of additives such as feed protein, mineral substances, zinc oxide and the like on the feed is greatly reduced after the mildew preventive is added to the feed. The non-ionic surfactant has good mixing performance with components such as propionic acid, ammonium propionate, sodium benzoate and the like, can obviously reduce the surface tension of water, is beneficial to uniformly dispersing all the components in the preparation process of the mildew preventive on one hand, and enables the free water in the feed to be distributed better and uniformly after the non-ionic surfactant is added into the feed on the other hand, the non-ionic surfactant is combined with the feed more closely, so that the water activity is obviously reduced, and the risk of feed mildew is obviously reduced.
Further, the carrier comprises one or more of silica powder, talcum powder, acidic calcium sulfate, montmorillonite powder and polylactic acid porous microspheres.
By adopting the technical scheme, the silicon dioxide powder, the talcum powder, the acidic calcium sulfate, the montmorillonite powder and the polylactic acid porous microspheres have excellent adsorption performance, and after the silicon dioxide powder, the talcum powder, the acidic calcium sulfate, the montmorillonite powder and the polylactic acid porous microspheres are adsorbed with active components with the mildew-proof effect in the mildew preventive, the release speed of active ingredients such as propionic acid can be greatly slowed down, the ineffective decomposition or loss of the active ingredients is reduced, the heat resistance stability and the durability of the mildew-proof effect of the mildew preventive are improved, and the mildew preventive is easier to store.
Further, the carrier comprises the following components in parts by weight,
20-30 parts of silicon dioxide powder
Montmorillonite powder 5-10 weight portions
5-10 parts of polylactic acid porous microspheres.
By adopting the technical scheme, the silicon dioxide powder has super-strong oil absorption, the montmorillonite powder also has excellent adsorption effect due to the special crystal structure, the polylactic acid porous microspheres are porous degradable environment-friendly materials, the carrier obtained by mixing according to the proportion has excellent adsorption effect on mildew-proof active ingredients such as propionic acid, the release speed of the active ingredients such as propionic acid is effectively slowed down, the ineffective loss is reduced, and the mildew-proof effect of the mildew preventive is more durable. Meanwhile, the mildew preventive prepared by the mixed carrier has the dual effects of fumigation mildew prevention and contact mildew prevention, and the mildew prevention effect is better.
Further, the nonionic surfactant is a mixture of polyoxyethylene sorbitan fatty acid ester, sorbitol, polyethylene glycol and propylene glycol.
The compound nonionic surfactant is selected, on one hand, the components are uniformly distributed in the preparation process of the mildew preventive, and on the other hand, the compound nonionic surfactant is added into the feed, so that the distribution of free water in the feed is better and uniform. The polyoxyethylene sorbitan fatty acid ester, sorbitol, polyethylene glycol and propylene glycol are all used in food industry or medicine, and the use safety is high.
Further, the composition also comprises 1-5 parts of polylysine by weight.
By adopting the technical scheme, polylysine has good antibacterial activity, has obvious synergistic effect when being mixed with propionic acid, ammonium propionate and sodium benzoate, and greatly improves antibacterial and mildewproof performance and mildewproof durability.
Further, the composition comprises 0.1 to 0.5 portion of eucommia ulmoides extract, 0.1 to 0.3 portion of bamboo leaf extract and 0.1 to 0.5 portion of liquorice extract in parts by weight.
By adopting the technical scheme, the eucommia ulmoides is natural Chinese herbal medicine, and the eucommia ulmoides extract obtained by extracting the eucommia ulmoides has a natural antibacterial effect and can play a role in enhancing animal immunity after being added into feed; the bamboo leaf extract has good antibacterial effect and has fragrant taste; the licorice extract is sweet in taste, can neutralize the bitter taste of the eucommia extract, has a certain detoxifying effect, and can improve the use safety of the mildew inhibitor.
The invention also aims to provide a preparation method of the efficient composite mildew inhibitor for the feed, and the mildew inhibitor prepared by the method has the advantages of good palatability, good mildew prevention effect and lasting mildew prevention effect.
The above object of the present invention is achieved by the following technical solutions:
a preparation method of a high-efficiency compound mildew preventive for feed comprises the following steps,
weighing: weighing 30-55 parts of propionic acid, 10-20 parts of ammonium propionate, 4-10 parts of sodium benzoate, 0.5-3 parts of nonionic surfactant and 17-55 parts of carrier according to the weight part ratio;
homogenizing: uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate and nonionic surfactant, and homogenizing to obtain homogenized liquid;
mixing: and uniformly mixing the prepared homogenized treatment solution and the weighed carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
By adopting the technical scheme, the effective components in the homogenized treatment solution obtained by homogenization are uniformly dispersed, and after the effective components are mixed with the carrier by a full-spray mixing method, the effective components can be fully adsorbed by the carrier, so that the mildew preventive with a lasting and efficient mildew-proof effect is obtained, and the mildew preventive is used for feed safety and can effectively prolong the storage time of the feed.
Further, the method comprises the following steps of,
weighing: weighing 30-55 parts of propionic acid, 10-20 parts of ammonium propionate, 4-10 parts of sodium benzoate, 0.5-3 parts of nonionic surfactant, 17-55 parts of carrier and 1-5 parts of polylysine according to the weight part ratio;
homogenizing: uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate, nonionic surfactant and polylysine, and homogenizing to obtain homogenized liquid;
mixing: and uniformly mixing the prepared homogenized treatment solution and the weighed carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
By adopting the technical scheme, the polylysine component is further added, so that the mildew-proof effect of the mildew inhibitor is further improved, and the mildew-proof and bacteriostatic universality is greatly improved.
Further, the method comprises the following steps of,
weighing: weighing 30-55 parts of propionic acid, 10-20 parts of ammonium propionate, 4-10 parts of sodium benzoate, 0.5-3 parts of nonionic surfactant, 17-55 parts of carrier, 1-5 parts of polylysine, 0.1-0.5 part of eucommia ulmoides extract, 0.1-0.3 part of bamboo leaf extract and 0.1-0.5 part of liquorice extract according to the weight part ratio; the carrier comprises 20-30 parts of silicon dioxide powder, 5-10 parts of montmorillonite powder and 5-10 parts of polylactic acid porous microspheres by weight;
homogenizing: uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate and nonionic surfactant, and homogenizing to obtain homogenized liquid;
pretreatment of a carrier: uniformly mixing the weighed carrier, polylysine, the eucommia ulmoides extract, the bamboo leaf extract and the liquorice extract at the rotating speed of 3500-4500rpm to obtain a pretreatment carrier;
mixing: and uniformly mixing the prepared homogenized treatment solution and the pretreatment carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
By adopting the technical scheme, the polylysine and the eucommia ulmoides extract, the bamboo leaf extract and the liquorice extract are fully mixed, then the mixture is mixed by a full-spray mixing method, the obtained mildew preventive has better mildew-proof effect and mildew-proof durability, and the mildew-proof durability of the feed can be effectively improved by adding a small amount of the mildew preventive, so that the feed is easier to store.
Further, in the homogenizing step, the weighed propionic acid, ammonium propionate, sodium benzoate and the nonionic surfactant are uniformly mixed, and then ultrasonic homogenizing treatment is carried out.
Through adopting above-mentioned technical scheme, supersound homogeneity is high-efficient, and the homogeneity effect is splendid, when doing benefit to follow-up full spray mixing, homogeneity treatment fluid and carrier intensive mixing do benefit to mould proof effective vehicle composition and are fully adsorbed by the carrier.
In summary, the invention includes at least one of the following beneficial technical effects:
1. the prepared mildew preventive has the functions of fumigation mildew prevention and contact mildew prevention, has excellent mildew prevention effect and good mildew prevention effect persistence, and can remarkably prolong a proper amount of storage time after a small amount of mildew preventive is added into the feed;
2. the silicon dioxide powder, the montmorillonite powder and the polylactic acid porous microspheres are mixed according to a specific proportion to serve as a carrier, so that the adsorption of active ingredients in the mildew preventive is excellent, the mildew preventive has good mildew resistance and durability after being added into feed, and the ineffective loss of the active ingredients in the mildew preventive is reduced;
3. the invention correspondingly discloses a preparation method of the efficient compound mildew preventive for the feed, and the mildew preventive prepared by the method has the advantages of good palatability, good mildew preventive effect and lasting mildew preventive effect.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
The sources of materials used in the examples are shown in table 1,
TABLE 1 raw materials Source Table
The preparation method of the polylactic acid porous microspheres used in the embodiments comprises the following steps:
(1) dissolving 5g of gelatin in 100ml of deionized water under the heating condition, cooling, adding 5g of ammonium bicarbonate, and stirring for dissolving to obtain an internal water phase; in addition, 100 g of polylactic acid is dissolved in 200ml of dichloromethane solution and transferred to a three-neck flask, an internal water phase is added, and the primary emulsion is stirred at the rotating speed of 1500rpm for 20 min; neutralizing with 5% acetic acid solution during colostrumization;
(2) preparing a gelatin solution with the mass fraction of 1% as an external water phase emulsifier;
(3) weighing 750ml of gelatin solution, adding into the primary emulsion, continuously stirring for 5h at 40-45 ℃, carrying out suction filtration, washing and drying to obtain the polylactic acid porous microspheres with the particle size of 20-100 microns.
Examples 1 to 3:
examples 1 to 3 each relate to a high-efficiency compound fungicide for feeds, which is prepared from propionic acid, ammonium propionate, sodium benzoate, a nonionic surfactant and a carrier according to the mixture ratio shown in table 2.
TABLE 2 raw material formulation tables of examples 1-3
Wherein the non-ionic surfactant is prepared by mixing tween-20, sorbitol, polyethylene glycol and propylene glycol according to the mass ratio of 1:0.5:1: 0.5; the carrier is prepared by mixing silica powder (reagent grade, particle size of 7-40 nm), montmorillonite KSF and self-made polylactic acid porous microspheres, and the specific formula is shown in Table 3.
TABLE 3 Carrier formulation Table for examples 1-3
The preparation method of the high-efficiency compound mildew preventive for the feed of the embodiment 1-3 comprises the following steps:
weighing the raw materials according to the proportion in the tables 2 and 3; uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate and nonionic surfactant, and performing ultrasonic homogenization treatment to obtain homogenized treatment liquid; and then, uniformly mixing the prepared homogenized treatment solution and the weighed carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
Examples 4 to 6:
examples 4 to 6 each relate to a high-efficiency compound fungicide for feeds, which is prepared from propionic acid, ammonium propionate, sodium benzoate, a nonionic surfactant, a carrier and polylysine according to the mixture ratio shown in table 4.
TABLE 4 raw material formulation tables of examples 4-6
Wherein the non-ionic surfactant is prepared by mixing tween-20, sorbitol, polyethylene glycol and propylene glycol according to the mass ratio of 1:0.5:1: 0.5; the carrier is prepared by mixing silica powder (reagent grade, particle size of 7-40 nm), montmorillonite KSF and self-made polylactic acid porous microspheres, and the specific formula is shown in Table 3.
TABLE 5 Carrier formulation Table for examples 4-6
The preparation method of the high-efficiency compound mildew preventive for the feed of the embodiment 4-6 comprises the following steps:
weighing the raw materials according to the proportion in the tables 4 and 5; uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate, nonionic surfactant and polylysine, and performing ultrasonic homogenization treatment to obtain homogenized treatment liquid; and then, uniformly mixing the prepared homogenized treatment solution and the weighed carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
Examples 7 to 9:
examples 7 to 9 are based on example 5, and are different from example 5 in that the formulation further contains an eucommia ulmoides extract, a bamboo leaf extract and a licorice root extract, and the specific raw material formulation is shown in table 6.
TABLE 6 raw material formulation tables of examples 7-9
Wherein the non-ionic surfactant is prepared by mixing tween-20, sorbitol, polyethylene glycol and propylene glycol according to the mass ratio of 1:0.5:1: 0.5; the carrier is prepared by mixing silicon dioxide powder (reagent grade, particle size of 7-40 nm), montmorillonite KSF and self-made polylactic acid porous microspheres, and the specific ratio is as follows: 25g of silicon dioxide powder, 8g of montmorillonite KSF and 7g of self-made polylactic acid porous microspheres.
The preparation method of the high-efficiency compound mildew preventive for the feed of the embodiment 7-9 comprises the following steps:
weighing raw materials according to the proportion in the table 6, uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate and nonionic surfactant, and performing ultrasonic homogenization treatment to obtain homogenized treatment liquid; uniformly mixing the weighed carrier, polylysine, the eucommia ulmoides extract, the bamboo leaf extract and the liquorice extract at the rotating speed of 3500-4500rpm to obtain a pretreatment carrier, wherein the rotating speed during mixing in examples 7-9 is 3500rpm, 4000rpm and 4500rpm in sequence; and finally, uniformly mixing the prepared homogenized treatment solution and the pretreatment carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
Examples 10 to 11:
examples 10 to 11 are based on example 9 and differ from example 9 only in that: the carrier used in example 10 included only talc and the carrier used in example 11 included only acidic calcium sulfate.
Examples 12 to 14:
examples 12 to 14 are based on example 9 and differ from example 9 only in that: the compositions of the used nonionic surfactants are different;
the nonionic surfactant used in example 12 was prepared by mixing tween-40, sorbitol, polyethylene glycol and propylene glycol in a mass ratio of 1:0.5:1: 0.5;
the nonionic surfactant used in example 13 was prepared by mixing tween-60, sorbitol, polyethylene glycol and propylene glycol in a mass ratio of 1:0.5:1: 0.5;
the nonionic surfactant used in example 14 was prepared by mixing tween-80, sorbitol, polyethylene glycol and propylene glycol in a mass ratio of 1:0.5:1: 0.5.
Comparative example 1:
comparative example 1 is an example of the chinese patent application publication with application publication No. CN102550800A, and its specific formulation is as follows:
50 percent of calcium propionate
15 percent of calcium carbonate
Lignin 15%
Chitosan 15%
Propionic acid 3%
2 percent of zinc sulfate
The configuration method comprises the following steps: the calcium propionate, the calcium carbonate, the lignin and the chitosan powder are fully and uniformly mixed, and then the propionic acid and the zinc sulfate are added and uniformly mixed to obtain the calcium propionate-calcium carbonate-chitosan composite material.
Comparative example 2:
the comparative example 2 is an example 1 disclosed in chinese patent with an authorization publication number CN103875916B, and the specific formulation thereof is as follows: 3 parts of pseudolarix, 12 parts of montmorillonite, 10.0 parts of phellodendron, 8.0 parts of scutellaria and 10 parts of cortex dictamni.
And (3) performance testing:
the antifungal agents of examples 1 to 14 and comparative examples 1 to 2 were used as test samples, and a feed was used for the antifungal test. The formula of the complete formula feed for the chickens of 0-4 weeks old for the test is as follows: 21.0 percent of crude protein, 1.00 percent of calcium, 0.65 percent of phosphorus and 0.37 percent of salt.
1) Test for mold preventive Effect
Under the aseptic condition, uniformly mixing the samples with the complete formula feed for chicken according to an equivalent increasing method by different contents to ensure that the concentrations of the mildew preventive in the samples are respectively 0.5%, 1.5%, 3.0% and 4.5%, and respectively filling the samples into sterilized plastic bags to avoid pollution; and putting a part of the blank feed without the mildew preventive into an incubator at 28 ℃ and 85% of humidity for culturing, and respectively measuring the mildew pollution condition of the blank feed when the blank feed is cultured for 15 days.
Weighing 25g of the feed sample in each subpackage bag by aseptic operation, and diluting by using sterilized normal saline to obtain 1: 10000 of diluent, sucking 1ml of diluent into a sterilization plate, making 3 controls for each material sample, then injecting a high-salt Chaochou culture medium which is cooled to about 45 ℃ into the plate, fully mixing, after agar is solidified, placing the plate upside down into a 28 ℃ incubator, culturing for 3 days, then starting observation, and observing and culturing for 1 week.
The components of the sterilized normal saline: 8.5 g of sodium chloride and 1000ml of distilled water. The preparation method comprises the following steps: heating to dissolve sodium chloride, packaging, and autoclaving at 121 deg.C.
The components of the high-salt Chaudou culture medium are as follows: 2g of sodium nitrate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 60g of sodium chloride, 30g of sucrose, 20g of agar and 1000ml of distilled water. The preparation method comprises the following steps: the ingredients were dissolved by heating and autoclaved at 115 ℃ for 30 minutes, adding agar if necessary.
The number of colonies on the plate was counted, and the average number of colonies on the 3 plates at the same dilution was multiplied by the dilution factor to obtain the number of molds contained in each gram of the test sample (ten thousand/g), and the test results are shown in Table 7.
2) Mildew resistance durability test
Under the aseptic condition, dividing each sample into four equal parts, uniformly mixing each part with the same content of the complete formula feed for the chickens, enabling the concentration of the mildew preventive in the material sample to be 3.0%, and respectively filling the material sample into sterilized plastic bags to avoid pollution; storing at 25 deg.C and 85% humidity for 7d, 15d, 28d, and 90d, respectively, and taking out the feed sample. And putting a part of the blank feed without the medicine into an incubator at 28 ℃ and 85% of humidity for culture, and respectively measuring the mould pollution condition of the blank feed when the blank feed is cultured for 15 days.
Weighing 25g of the feed sample in each subpackage bag by aseptic operation, and diluting by using sterilized normal saline to obtain 1: 10000 of diluent, sucking 1ml of diluent into a sterilization plate, making 3 controls for each material sample, then injecting a high-salt Chaochou culture medium which is cooled to about 45 ℃ into the plate, fully mixing, after agar is solidified, placing the plate upside down into a 28 ℃ incubator, culturing for 3 days, then starting observation, and observing and culturing for 1 week.
The components of the sterilized normal saline: 8.5 g of sodium chloride and 1000ml of distilled water. The preparation method comprises the following steps: heating to dissolve sodium chloride, packaging, and autoclaving at 121 deg.C.
The components of the high-salt Chaudou culture medium are as follows: 2g of sodium nitrate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 60g of sodium chloride, 30g of sucrose, 20g of agar and 1000ml of distilled water. The preparation method comprises the following steps: the ingredients were dissolved by heating and autoclaved at 115 ℃ for 30 minutes, adding agar if necessary.
The number of colonies on the plate was counted, and the average number of colonies on the 3 plates at the same dilution was multiplied by the dilution factor to obtain the number of molds contained in each gram of the test sample (number of molds unit: ten thousand/g), and the test results are shown in Table 8.
TABLE 7 mildew-proof effect test results table
TABLE 8 mildew resistance durability test results table
From the experimental data in tables 7 and 8, it can be seen that: the efficient composite mildew inhibitor for the feed has excellent mildew-proof effect and mildew-proof durability, the mildew-proof effect of the efficient composite mildew inhibitor is superior to that of comparative example 1 and comparative example 2 under the same condition, and experimental data show that the storage resistance of the mildew inhibitor is better, the weakening degree of the mildew-proof effect is not large after the efficient composite mildew inhibitor is added and the mildew-proof effect is durable.
The experimental data of comparative examples 1-3 and examples 4-6 show that the addition of polylysine has a promoting effect on the improvement of the mildew-proof effect and the mildew-proof durability of the mildew-proof agent; the experimental data of comparative examples 4-6 and examples 7-9 show that the mildew-proof effect and the mildew-proof durability of the mildew inhibitor can be further improved after the eucommia ulmoides extract, the bamboo leaf extract and the liquorice extract which are added according to the specific mixture ratio of the application are added; the experimental data of comparative example 9 and examples 10 to 11 show that the effect of selecting a single carrier is inferior to that of a mixed carrier, and the carrier prepared by mixing silica powder, montmorillonite powder and self-made polylactic acid porous particles according to a specific ratio in the application has a promoting effect on improving the mildew-proof effect and the mildew-proof durability. Meanwhile, as can be seen from the experimental data of comparative example 9 and examples 12 to 14, different kinds of tween can be selected for use in the present invention, and the overall effect is not greatly different.
The above-mentioned embodiments are merely illustrative and not restrictive, and those skilled in the art can modify the above-mentioned embodiments as required after reading this specification without any inventive contribution, but only if the claims of the present invention are protected by patent laws.
Claims (10)
1. The efficient composite mildew inhibitor for the feed is characterized by comprising the following components in parts by weight: comprises the following components in parts by weight,
30-55 parts of propionic acid
10-20 parts of ammonium propionate
4-10 parts of sodium benzoate
0.5-3 parts of nonionic surfactant
17-55 parts of a carrier.
2. The highly effective compound fungicide for feed according to claim 1, characterized in that: the carrier comprises one or more of silicon dioxide powder, talcum powder, acidic calcium sulfate, montmorillonite powder and polylactic acid porous microspheres.
3. The highly effective compound fungicide for feed according to claim 2, characterized in that: the carrier comprises 20-30 parts of silicon dioxide powder, 5-10 parts of montmorillonite powder and 5-10 parts of polylactic acid porous microspheres by weight.
4. The highly effective compound fungicide for feed according to claim 1, characterized in that: the nonionic surfactant is a mixture of polyoxyethylene sorbitan fatty acid ester, sorbitol, polyethylene glycol and propylene glycol.
5. The highly efficient composite fungicide for feeds according to any one of claims 1 to 4, characterized in that: according to the weight portion, the composition also comprises 1-5 portions of polylysine.
6. The highly efficient composite fungicide for feeds according to any one of claims 1 to 4, characterized in that: 0.1 to 0.5 portion of eucommia bark extract, 0.1 to 0.3 portion of bamboo leaf extract and 0.1 to 0.5 portion of liquorice extract are also included according to the weight portion.
7. The preparation method of the efficient compound mildew preventive for feed according to claim 1, characterized by comprising the following steps: comprises the following steps of (a) carrying out,
weighing: weighing 30-55 parts of propionic acid, 10-20 parts of ammonium propionate, 4-10 parts of sodium benzoate, 0.5-3 parts of nonionic surfactant and 17-55 parts of carrier according to the weight part ratio;
homogenizing: uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate and nonionic surfactant, and homogenizing to obtain homogenized liquid;
mixing: and uniformly mixing the prepared homogenized treatment solution and the weighed carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
8. The preparation method of the efficient compound mildew preventive for feed according to claim 7, characterized by comprising the following steps: comprises the following steps of (a) carrying out,
weighing: weighing 30-55 parts of propionic acid, 10-20 parts of ammonium propionate, 4-10 parts of sodium benzoate, 0.5-3 parts of nonionic surfactant, 17-55 parts of carrier and 1-5 parts of polylysine according to the weight part ratio;
homogenizing: uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate, nonionic surfactant and polylysine, and homogenizing to obtain homogenized liquid;
mixing: and uniformly mixing the prepared homogenized treatment solution and the weighed carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
9. The preparation method of the efficient compound mildew preventive for feed according to claim 7, characterized by comprising the following steps: comprises the following steps of (a) carrying out,
weighing: weighing 30-55 parts of propionic acid, 10-20 parts of ammonium propionate, 4-10 parts of sodium benzoate, 0.5-3 parts of nonionic surfactant, 17-55 parts of carrier, 1-5 parts of polylysine, 0.1-0.5 part of eucommia ulmoides extract, 0.1-0.3 part of bamboo leaf extract and 0.1-0.5 part of liquorice extract according to the weight part ratio; the carrier comprises 20-30 parts of silicon dioxide powder, 5-10 parts of montmorillonite powder and 5-10 parts of polylactic acid porous microspheres by weight;
homogenizing: uniformly mixing the weighed propionic acid, ammonium propionate, sodium benzoate and nonionic surfactant, and homogenizing to obtain homogenized liquid;
pretreatment of a carrier: uniformly mixing the weighed carrier, polylysine, the eucommia ulmoides extract, the bamboo leaf extract and the liquorice extract at the rotating speed of 3500-4500rpm to obtain a pretreatment carrier;
mixing: and uniformly mixing the prepared homogenized treatment solution and the pretreatment carrier by adopting a full-spray mixing method to obtain the efficient composite mildew preventive for the feed.
10. The method for preparing the highly effective compound fungicide for feeds according to any one of claims 7 to 9, characterized in that: in the homogenizing step, the weighed propionic acid, ammonium propionate, sodium benzoate and nonionic surfactant are uniformly mixed, and then ultrasonic homogenizing treatment is carried out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911307153.XA CN112971001A (en) | 2019-12-18 | 2019-12-18 | Efficient composite mildew inhibitor for feed and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911307153.XA CN112971001A (en) | 2019-12-18 | 2019-12-18 | Efficient composite mildew inhibitor for feed and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112971001A true CN112971001A (en) | 2021-06-18 |
Family
ID=76343801
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911307153.XA Pending CN112971001A (en) | 2019-12-18 | 2019-12-18 | Efficient composite mildew inhibitor for feed and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112971001A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115137035A (en) * | 2022-07-14 | 2022-10-04 | 浙江维精科技有限公司 | Preparation method of montmorillonite mildew preventive |
| CN115336671A (en) * | 2022-09-28 | 2022-11-15 | 南通奥凯生物技术开发有限公司 | Calcium propionate-containing high-purity feed mildew preventive, preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647595A (en) * | 2009-08-28 | 2010-02-17 | 南通中牧生物科技有限公司 | Alanine compound mildew preventive and preparation method thereof |
| CN103168974A (en) * | 2013-03-25 | 2013-06-26 | 重庆民泰香料化工有限责任公司 | Liquid mildewproof agent for feed and method for avoiding mildew of feed |
| CN103875916A (en) * | 2012-12-20 | 2014-06-25 | 信宜市金方子生物科技有限公司 | Chinese herbal medicine-type feed mildew inhibitor |
| CN104115998A (en) * | 2014-07-23 | 2014-10-29 | 重庆威士化工有限公司 | Feed water adjusting agent and method for restoring normal water of feed |
-
2019
- 2019-12-18 CN CN201911307153.XA patent/CN112971001A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647595A (en) * | 2009-08-28 | 2010-02-17 | 南通中牧生物科技有限公司 | Alanine compound mildew preventive and preparation method thereof |
| CN103875916A (en) * | 2012-12-20 | 2014-06-25 | 信宜市金方子生物科技有限公司 | Chinese herbal medicine-type feed mildew inhibitor |
| CN103168974A (en) * | 2013-03-25 | 2013-06-26 | 重庆民泰香料化工有限责任公司 | Liquid mildewproof agent for feed and method for avoiding mildew of feed |
| CN104115998A (en) * | 2014-07-23 | 2014-10-29 | 重庆威士化工有限公司 | Feed water adjusting agent and method for restoring normal water of feed |
Non-Patent Citations (1)
| Title |
|---|
| 旷春桃，等: "植物源天然防霉剂的研究与应用现状", 《湖南饲料》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115137035A (en) * | 2022-07-14 | 2022-10-04 | 浙江维精科技有限公司 | Preparation method of montmorillonite mildew preventive |
| CN115137035B (en) * | 2022-07-14 | 2024-04-16 | 浙江维精科技有限公司 | Preparation method of montmorillonite mildew inhibitor |
| CN115336671A (en) * | 2022-09-28 | 2022-11-15 | 南通奥凯生物技术开发有限公司 | Calcium propionate-containing high-purity feed mildew preventive, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112971001A (en) | Efficient composite mildew inhibitor for feed and preparation method thereof | |
| JP3026141B2 (en) | Pollution-resistant animal feed, method for producing the feed, and method for raising livestock | |
| EP3030248A1 (en) | Tall oil fatty acid for use in treatment and animal feed supplements and compositions | |
| KR20160016926A (en) | Feed supplement and its use | |
| CN104288465B (en) | Prevent the combination of enteric plant essential oil and its preparation process of grice diarrhoea | |
| CN105901356A (en) | Composite nano-zinc oxide additive and preparation method and application thereof | |
| CN101070513A (en) | Farm-chemical biological decomposing composition and preparing method | |
| CN100592872C (en) | Disinfecting agent for rearing house, silkworm tool, silkworm body, silkworm seat and mulberry leaves | |
| CN111388498B (en) | Spectinolincomycin hydrochloride soluble powder capable of being mutually dissolved with oil seedlings after being dissolved in water and preparation method thereof | |
| CN116983392A (en) | Soluble granule containing colistin sulfate and preparation method thereof | |
| CN103636677A (en) | Disinfectant for killing silkworm viruses | |
| KR102488475B1 (en) | Multipurpose Water-Based Cleaner Composition | |
| CN109833296A (en) | Can the new merit chigger disease of prevention and control plants essential oil microemulsion formulation and preparation method thereof | |
| JPH11506308A (en) | Compositions for reducing odor from animal waste | |
| CN113383790A (en) | Environment-friendly potassium hydrogen persulfate compound disinfectant and production process thereof | |
| CN113475530A (en) | Stable disinfectant and preparation method thereof | |
| EP2188214B1 (en) | Silica dispersion | |
| DE60219177T3 (en) | VACCINE COMPOSITION CONTAINING AT LEAST TWO IMMUNOGENES, AN IMMUNOGEN WITH ADJUVAN, WITHOUT ADJUVANT IMMUNOGEN | |
| CN106538625A (en) | A kind of biosynthesis Nano Silver fishery disinfectant | |
| CN106173802A (en) | A kind of NEW TYPE OF COMPOSITE Midew preventive for feed and its preparation method and application | |
| CN1173998A (en) | Compound feed antifermenting and antioxidizing agent and its production process | |
| CN106359450A (en) | Livestock and poultry farm environment improver and preparation method thereof | |
| CN105778260B (en) | A kind of preparation method of the fresh-keeping film master batch of loquat containing rare earth compounding | |
| RU2779969C1 (en) | Feed additive | |
| CN116139178B (en) | Livestock dry powder with disinfection function and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210618 |