CN112970509B - Method for cultivating ganoderma lucidum - Google Patents
Method for cultivating ganoderma lucidum Download PDFInfo
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- CN112970509B CN112970509B CN201911212873.8A CN201911212873A CN112970509B CN 112970509 B CN112970509 B CN 112970509B CN 201911212873 A CN201911212873 A CN 201911212873A CN 112970509 B CN112970509 B CN 112970509B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The method for cultivating the ganoderma lucidum comprises the steps of putting female parent ganoderma lucidum into an aqueous solution, controlling the water temperature to be 13-15 ℃, standing for 7-10 days, and then carrying out coarse filtration to obtain a slime fission cell solution for later use; replacing the female parent ganoderma lucidum with a new water solution, and controlling the temperature to be between 18 and 26 ℃ to obtain a fungal spore and bacterial propagule solution; mixing the slime fission cell solution with the fungal spore and bacterial propagule solution, adding food-grade cold gel into the mixed solution, and allowing the slime and fungal bacteria in the artificially synthesized jelly ganoderma lucidum to continue to generate the ganoderma lucidum gradually changing into natural state. The method realizes the artificial domestication of the wild ganoderma lucidum, and arouses the growth characteristics of the wild ganoderma lucidum; thereby laying a foundation for the rapid transformation of the ganoderma lucidum into normal production and being beneficial to the large-scale production and application of the ganoderma lucidum. The formed ganoderma lucidum mycelia are bright in color, good in shape, rich in nutrition and excellent in quality.
Description
Technical Field
The invention belongs to the technical field of biological breeding, and particularly relates to a method for cultivating ganoderma lucidum.
Background
Ganoderma lucidum is a large-scale slime mold complex, and is also a polymer of slime mold, fungi and bacteria. In ancient times, the environment of the people is changed due to climate and geographic environment, and the change disappears in the visual field of the people. At present, the ganoderma lucidum is generally found underground, in a deep mountain or in a river channel far away from people, and is rare. Modern science finds that ganoderma lucidum contains rich nutrient components and has a function of replacing the health of a human body. Because ganoderma lucidum is rare, the market price is high, and the market development cost is high.
At present, the ganoderma lucidum industry is gradually rising, the demand for ganoderma lucidum is more and more, and thus, the demand for ganoderma lucidum seed sources is increasingly increased. However, there are very few wild ganoderma lucidum in nature, so that the wild ganoderma lucidum resource needs to be fully developed and utilized, and for the situation, the applicant summarizes the following cultivation method through two decades of experience.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for cultivating ganoderma lucidum.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a method for cultivating ganoderma lucidum comprises stages of ganoderma lucidum myxomycete fission cell cultivation, fungal spore and bacterial propagule cultivation and mixed cultivation, and specifically comprises the following steps:
(1) culturing myxomycete fission cells of ganoderma lucidum: putting the cleaned mother ganoderma lucidum into mountain spring water or mineral water solution with the pH value of 6.5-6.8, controlling the water temperature to be 13-15 ℃, standing for 7-10 days, and then sending the water solution containing the slime mold cells into a molecular sieve for coarse filtration, so that slime mold cells in the solution are collected, and slime mold cell solution is obtained for later use;
(2) fungal spore and bacterial propagule cultivation: replacing the female parent ganoderma lucidum with a new water solution, wherein the pH value of the water solution is 6.5-6.8, the temperature is controlled between 18 ℃ and 26 ℃, the culture period of the fungal spores and the bacterial propagules is 5 days, and thus a fungal spore and bacterial propagule solution is obtained;
(3) mixed cultivation: mixing the slime fission cell solution with the fungal spore and bacterial propagule solution, simultaneously adding food-grade cold glue into the mixed solution, after 1 month, forming fixed jelly with different shapes by the liquid, taking out the solid jelly and putting the solid jelly together with the original ganoderma lucidum into a water solution of mountain spring water or mineral water with the pH value of 6.5-6.8 for co-cultivation, continuously generating slime and fungal bacteria in the artificially synthesized jelly ganoderma lucidum, and enabling the secreted slime fission cell, fungal spore and bacterial propagule to wrap the artificially synthesized jelly to gradually become the natural ganoderma lucidum.
In the course of culturing myxomycete fission cells of ganoderma lucidum, medical stone is placed in the aqueous solution.
The cultivation of the fungal spores and the bacterial propagules is carried out in a dark anaerobic environment.
The method realizes the artificial domestication of the wild ganoderma lucidum, and arouses the growth characteristics of the wild ganoderma lucidum; thereby laying a foundation for the rapid transformation of the ganoderma lucidum into normal production and being beneficial to the large-scale production and application of the ganoderma lucidum. The formed ganoderma lucidum mycelia are bright in color, good in shape, rich in nutrition and excellent in quality.
Detailed Description
A method for cultivating ganoderma lucidum comprises stages of ganoderma lucidum slime fission cell cultivation, fungal spore and bacterial propagule cultivation and mixed cultivation, and specifically comprises the following steps:
(1) culturing myxomycete fission cells of ganoderma lucidum: putting the cleaned mother ganoderma lucidum into mountain spring water or mineral water solution with the pH value of 6.5-6.8, controlling the water temperature to be 13-15 ℃, ensuring that the growth speed of slime bacteria in the temperature range is high, quickly cracking slime bacteria growing cells, discharging the cracked slime cells into the mountain spring water or mineral water solution, putting medical stone blocks into the water solution, placing for 7-10 days, then sending the water solution containing the slime cells into a molecular sieve for coarse filtration, and collecting the slime cells in the solution to obtain slime cell solution for later use;
(2) fungal spore and bacterial propagule cultivation: replacing a new aqueous solution for the female parent ganoderma lucidum, wherein the pH value of the aqueous solution is 6.5-6.8, the temperature is controlled between 18 ℃ and 26 ℃, the growth speed of fungi and bacteria is high in the temperature range, the culture period of the fungi spores and the bacteria propagules is 5 days, and the solution of the fungi spores and the bacteria propagules is obtained, and the culture of the fungi spores and the bacteria propagules is carried out in a dark anaerobic environment;
(3) mixed cultivation: mixing the slime fission cell solution with the fungal spore and bacterial propagule solution, simultaneously adding food-grade cold glue into the mixed solution, after 1 month, forming fixed jelly with different shapes by the liquid, taking out the solid jelly and putting the solid jelly together with the original ganoderma lucidum into a water solution of mountain spring water or mineral water with the pH value of 6.5-6.8 for co-cultivation, continuously generating slime and fungal bacteria in the artificially synthesized jelly ganoderma lucidum, and enabling the secreted slime fission cell, fungal spore and bacterial propagule to wrap the artificially synthesized jelly to gradually become the natural ganoderma lucidum.
Claims (2)
1. A method for cultivating ganoderma lucidum, which is characterized by comprising the stages of cultivating myxomycete fission cells of ganoderma lucidum, cultivating fungal spores and bacterial propagules and mixing and cultivating, and the specific method comprises the following steps:
(1) culturing myxomycete fission cells of ganoderma lucidum: putting the cleaned mother ganoderma lucidum into mountain spring water or mineral water solution with the pH value of 6.5-6.8, controlling the water temperature to be 13-15 ℃, standing for 7-10 days, then sending the water solution containing the slime mold cells into a molecular sieve for coarse filtration, collecting slime mold cells in the solution, and obtaining slime mold cell solution for later use;
(2) fungal spore and bacterial propagule cultivation: culturing the fungal spores and the bacterial propagules in a dark anaerobic environment, replacing a new aqueous solution for the female parent ganoderma lucidum, controlling the pH value of the aqueous solution to be 6.5-6.8 and the temperature to be 18-26 ℃, and culturing the fungal spores and the bacterial propagules for 5 days to obtain a solution of the fungal spores and the bacterial propagules;
(3) mixed cultivation: mixing the slime fission cell solution with the fungal spore and bacterial propagule solution, simultaneously adding food-grade cold glue into the mixed solution, after 1 month, forming fixed jelly with different shapes by the liquid, taking out the solid jelly and putting the solid jelly together with the original meat ganoderma lucidum into the aqueous solution of mountain spring water or mineral water with the pH value of 6.5-6.8 for co-cultivation, continuously generating slime and fungal bacteria in the artificially synthesized jelly meat ganoderma lucidum after half a year to one year, and wrapping the artificially synthesized jelly by the secreted slime fission cell, fungal spore and bacterial propagule to gradually become the natural meat ganoderma lucidum.
2. The method according to claim 1, wherein the mashi stone is placed in the aqueous solution during culturing myxomycete fission cells of the ganoderma lucidum.
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| CN201911212873.8A CN112970509B (en) | 2019-12-02 | 2019-12-02 | Method for cultivating ganoderma lucidum |
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| CN114711091B (en) * | 2022-05-13 | 2023-06-30 | 地宝集团有限公司 | Method for artificially cultivating ganoderma lucidum |
| CN116042403A (en) * | 2023-02-06 | 2023-05-02 | 地宝集团有限公司 | Meat ganoderma lucidum breeding stabilizer and using method thereof |
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| JP2014158441A (en) * | 2013-02-20 | 2014-09-04 | Nikken Sohonsha Corp | Cultivation method of ganoderma lucidum having novel bioactivity |
| CN104186197A (en) * | 2014-06-16 | 2014-12-10 | 刘玉勤 | Cultivation method of ganoderma lucidum casein film crystal |
| CN106212046A (en) * | 2016-07-28 | 2016-12-14 | 河北岁源饮品有限公司 | A kind of Ganoderma breeding method and the method for manufactured meat Ganoderma water |
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| CN107361354A (en) * | 2017-08-30 | 2017-11-21 | 武清堂 | A kind of slender cytosol unimolecule water and preparation method for removing blood free radicals |
| CN108713447A (en) * | 2018-06-01 | 2018-10-30 | 韩秀花 | A kind of high altitude localities local tyrant meat Mythic Fungus cultivation method and its health products preparation method |
| CN110075247A (en) * | 2019-06-05 | 2019-08-02 | 潘玉萍 | A kind of herbal mixture fermentation liquid and preparation method thereof |
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2019
- 2019-12-02 CN CN201911212873.8A patent/CN112970509B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101603010A (en) * | 2009-06-22 | 2009-12-16 | 贾永财 | A kind of fast cultivation method for Taisui |
| JP2014158441A (en) * | 2013-02-20 | 2014-09-04 | Nikken Sohonsha Corp | Cultivation method of ganoderma lucidum having novel bioactivity |
| CN104186197A (en) * | 2014-06-16 | 2014-12-10 | 刘玉勤 | Cultivation method of ganoderma lucidum casein film crystal |
| CN106282024A (en) * | 2015-05-14 | 2017-01-04 | 河北圣雪大成制药有限责任公司 | A kind of method improving production polymyxin bacterial strain forward mutation probability |
| CN106212046A (en) * | 2016-07-28 | 2016-12-14 | 河北岁源饮品有限公司 | A kind of Ganoderma breeding method and the method for manufactured meat Ganoderma water |
| CN106635660A (en) * | 2017-02-24 | 2017-05-10 | 北京昆仑奇芝生物科技发展有限公司 | Hymenomycete active bacterium fermented liquor and preparation method thereof |
| CN107361354A (en) * | 2017-08-30 | 2017-11-21 | 武清堂 | A kind of slender cytosol unimolecule water and preparation method for removing blood free radicals |
| CN108713447A (en) * | 2018-06-01 | 2018-10-30 | 韩秀花 | A kind of high altitude localities local tyrant meat Mythic Fungus cultivation method and its health products preparation method |
| CN110075247A (en) * | 2019-06-05 | 2019-08-02 | 潘玉萍 | A kind of herbal mixture fermentation liquid and preparation method thereof |
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Address after: 062,650 Gengguantun Village, Qingzhou Town, Qingxian County, Cangzhou City, Hebei Province Patentee after: Hebei Xinghua Shengyuan Biotechnology Co.,Ltd. Address before: 062650 east side of North Ring Railway, Qingzhou Town, Qingxian County, Cangzhou City, Hebei Province Patentee before: Hebei Xinghua Shengyuan Biotechnology Co.,Ltd. |