CN105210873A - A kind of drop of water petiole seedling-cultivating method - Google Patents
A kind of drop of water petiole seedling-cultivating method Download PDFInfo
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- CN105210873A CN105210873A CN201510667171.4A CN201510667171A CN105210873A CN 105210873 A CN105210873 A CN 105210873A CN 201510667171 A CN201510667171 A CN 201510667171A CN 105210873 A CN105210873 A CN 105210873A
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Abstract
The present invention relates to botanical seedling culturing technical field, be specifically related to a kind of drop of water petiole seedling-cultivating method.Get the fresh petiole of wild drop of water, shape of cutting into chunks, concentration be 400ppm, temperature is soak 24-28 hour in the D-Fructose solution of 10-15 DEG C; Section shape petiole is placed in flowing water and rinses well; Section shape petiole is inoculated in aseptic culture medium, 1-2 days is cultivated at 15-18 DEG C, section shape petiole is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20-22 DEG C, illumination is 18-20klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, silica flour 20 parts, ferrous sulfate 0.05-0.08 part, 500 parts, water.
Description
Technical field
The present invention relates to botanical seedling culturing technical field, be specifically related to a kind of drop of water petiole seedling-cultivating method.
Background technology
Drop of water belongs to Araeceae draft, medicinal underground bulb, have eliminating dampness and eliminating phlegm, effect of dissolving lump and resolving mass, to the wet phlegm cold drink for the treatment of, vomiting, gastric disorder causing nausea, cough, phlegm is many, headache egersis etc. has good effect, this product consumption is large, also be export large kind throughout the year, Ju is national Ge great Yao city and the analysis of producing region market investigation statistics over 10 years, drought tuber of pinellia supply demand gap is larger, tendency is powerful, drop of water happiness shade environment, in recent years reclamation of wasteland deforestation, a large amount of use herbicide, that its growing environment goes to pot and causes wild area to reduce year by year, output falls sharply thereupon, and artificial planting is also because planting cost is high, the reasons such as miscarriage, output is always lower.
Summary of the invention
The object of the present invention is to provide a kind of drop of water petiole seedling-cultivating method.
A kind of drop of water petiole seedling-cultivating method, it comprises the steps:
1, get the fresh petiole of wild drop of water, be cut into the section shape that length is 1-1.5cm, after aseptic water washing, concentration be 400ppm, temperature is soak 24-28 hour in the D-Fructose solution of 10-15 DEG C;
2, section shape petiole is placed in flowing water rinses well;
3, section shape petiole is inoculated in aseptic culture medium, 1-2 days is cultivated at 15-18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, vitamin c 1-2 part, distilled water 1000 parts;
4, section shape petiole is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20-22 DEG C, illumination is 18-20klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, silica flour 20 parts, ferrous sulfate 0.05-0.08 part, 500 parts, water.
Petiole seedling-cultivating method of the present invention can reduce the content of ABA in petiole fast, breaking dormancy effect, promotes the fast healing and taking root of drop of water petiole, improves rooting rate, cultivate in root media and can grow healthy and strong root system in 40 days, without the need to can directly transplant to land for growing field crops through hardening after taking root, emerge after transplanting fast, emergence rate be high, emergence rate can reach 90.86%, the standardized quick fast-growing that can realize drop of water seedling is produced, and shortens crop cycle, increases economic efficiency.
Embodiment
Embodiment 1,1, get the fresh petiole of wild drop of water, be cut into the section shape that length is 1cm, after aseptic water washing, concentration be 400ppm, temperature is soak 24 hours in the D-Fructose solution of 10 DEG C; 2, section shape petiole is placed in flowing water rinses well; 3, section shape petiole is inoculated in aseptic culture medium, cultivate 1 day at 15 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, vitamin c 1-2 part, distilled water 1000 parts; 4, section shape petiole is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20 DEG C, illumination is 18klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, silica flour 20 parts, 0.05 part, ferrous sulfate, 500 parts, water.
The section of obtaining shape petiole average cultivation in root media can be taken root for 40 days, and rooting rate can reach 96.15%, transplants to land for growing field crops after taking root, and adds up survival rate after 3 weeks, and survival rate can reach 88.31%.
Embodiment 2,1, get the fresh petiole of wild drop of water, be cut into the section shape that length is 1.5cm, after aseptic water washing, concentration be 400ppm, temperature is soak 28 hours in the D-Fructose solution of 15 DEG C; 2, section shape petiole is placed in flowing water rinses well; 3, section shape petiole is inoculated in aseptic culture medium, cultivate 2 days at 18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, vitamin c 1-2 part, distilled water 1000 parts; 4, section shape petiole is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 22 DEG C, illumination is 20klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, silica flour 20 parts, 0.08 part, ferrous sulfate, 500 parts, water.
The section of obtaining shape petiole average cultivation in root media can be taken root for 39.2 days, and rooting rate can reach 96.98%, transplants to land for growing field crops after taking root, and adds up survival rate after 3 weeks, and survival rate can reach 90.86%.
Embodiment 3,1, get the fresh petiole of wild drop of water, be cut into the section shape that length is 1.5cm, after aseptic water washing, concentration be 400ppm, temperature is soak 24-2826 hour in the D-Fructose solution of 13 DEG C; 2, section shape petiole is placed in flowing water rinses well; 3, section shape petiole is inoculated in aseptic culture medium, cultivate 2 days at 16 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, vitamin c 1-2 part, distilled water 1000 parts; 4, section shape petiole is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 21 DEG C, illumination is 19klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, silica flour 20 parts, 0.06 part, ferrous sulfate, 500 parts, water.
Obtain block petiole average cultivation in root media can take root for 39.8 days, rooting rate can reach 96.38%, transplants to land for growing field crops after taking root, and adds up survival rate after 3 weeks, and survival rate can reach 89.69%.
Claims (1)
1. a drop of water petiole seedling-cultivating method, is characterized in that, it comprises the steps:
1), get the fresh petiole of wild drop of water, be cut into the section shape that length is 1-1.5cm, after aseptic water washing, concentration be 400ppm, temperature is soak 24-28 hour in the D-Fructose solution of 10-15 DEG C;
2), section shape petiole is placed in flowing water rinses well;
3), section shape petiole is inoculated in aseptic culture medium, 1-2 days is cultivated at 15-18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, vitamin c 1-2 part, distilled water 1000 parts;
4), section shape petiole is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20-22 DEG C, illumination is 18-20klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, silica flour 20 parts, ferrous sulfate 0.05-0.08 part, 500 parts, water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510667171.4A CN105210873A (en) | 2015-10-16 | 2015-10-16 | A kind of drop of water petiole seedling-cultivating method |
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| CN201510667171.4A CN105210873A (en) | 2015-10-16 | 2015-10-16 | A kind of drop of water petiole seedling-cultivating method |
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| CN105210873A true CN105210873A (en) | 2016-01-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106359054A (en) * | 2016-09-29 | 2017-02-01 | 秦士勇 | Rapid seedling growing method of acer monoes suitable for being used in Anhui northern area |
Citations (3)
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| CN1602678A (en) * | 2004-11-04 | 2005-04-06 | 浙江省农业科学院 | Quick reproduction method for cordate halfsummer tissue culture |
| CN101803571A (en) * | 2010-03-30 | 2010-08-18 | 浙江理工大学 | Tissue culture rapid propagation method of Rhizoma Typhonii Flagelliformis |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method for pinellia tuber plant |
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2015
- 2015-10-16 CN CN201510667171.4A patent/CN105210873A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1602678A (en) * | 2004-11-04 | 2005-04-06 | 浙江省农业科学院 | Quick reproduction method for cordate halfsummer tissue culture |
| CN101803571A (en) * | 2010-03-30 | 2010-08-18 | 浙江理工大学 | Tissue culture rapid propagation method of Rhizoma Typhonii Flagelliformis |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method for pinellia tuber plant |
Non-Patent Citations (1)
| Title |
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| 郁建新: "滴水珠的组培培养和植株再生", 《植物生理学通讯》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106359054A (en) * | 2016-09-29 | 2017-02-01 | 秦士勇 | Rapid seedling growing method of acer monoes suitable for being used in Anhui northern area |
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