CN112913612A - Method for improving content of saponin VI in cultivated paris polyphylla - Google Patents
Method for improving content of saponin VI in cultivated paris polyphylla Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a method for improving the content of cultivated paris polyphylla saponin VI, which is used for keeping the field water holding capacity of soil at about 50 percent; artificially inoculating ivory leucocyte enzyme, cryptococcus enzyme and simple bacillus, and co-culturing the microbe and Paris polyphylla. Different microorganisms are inoculated in the process of cultivating the paris polyphylla, and moderate drought treatment is assisted, so that the content of saponin VI is improved, and the quality of the paris polyphylla is improved; meanwhile, the chlorophyll content of the paris polyphylla seedlings can be improved, and the growth of the paris polyphylla seedlings is promoted.
Description
Technical Field
The invention relates to the technical field of Chinese herbal medicine cultivation, in particular to a method for improving the content of saponin VI in cultivated paris polyphylla.
Background
Paris polyphylla belongs to Paris of Liliaceae, has long medicinal history, and is one of medicinal plants which are most representative and occupy the leading position of industrial utilization in western regions of China. Modern pharmacological studies show that the paris polyphylla has the effects of promoting blood circulation, removing blood stasis, relieving swelling and pain, cooling liver, arresting convulsion, clearing heat, detoxifying, resisting cancer, resisting tumor, regulating immunity and the like, is commonly used for treating traumatic injury and snake and insect bite, and is clinically applied to tumor, parotitis, tonsillitis, respiratory tract infection and the like. Because of its unique curative effect, the Chinese patent medicine and health-care product made up by using said Chinese patent medicine as raw material can be up to hundreds of kinds. In Chinese pharmacopoeia (2015), the total content of effective components of rhizoma paridis saponin I, II, VI and VII of rhizoma paridis is not less than 0.6% as the standard for qualified rhizoma paridis medicinal material.
At present, the demand of Yunnan manyleaf paris rhizome in Yunnan province is more than 5000 tons every year, but because the regeneration period of Yunnan manyleaf paris rhizome is long (8 to 10 years), and the seeds have the physiological characteristic of 'secondary dormancy', the emergence rate of seed propagation is lower, and the asexual propagation technology (such as tissue culture) does not make breakthrough progress at present, so that the wild manyleaf paris rhizome resources are reduced day by day for a long time because the wild manyleaf paris rhizome resources are used as raw materials in both folks and enterprises.
In order to solve the market situation that the supply of Chinese medicinal materials of the paris polyphylla is not in short supply, the paris polyphylla planting is vigorously developed in various places in Yunnan province in recent years, and the planting area of the paris polyphylla in the whole Yunnan province is conservatively estimated to be about 14 ten thousand mu. Along with the continuous expansion of the planting area of the paris polyphylla, the quality problem generated increasingly attracts enterprise attention. The paris polyphylla variety mainly cultivated in Yunnan province is almost lack of steroid paridis saponin VI, and the loss of the steroid saponin VI of the paris polyphylla greatly reduces the quality of medicinal materials for cultivating the paris polyphylla and severely restricts the benign development of related pharmaceutical enterprises.
Disclosure of Invention
Aiming at the problems, the invention provides a method for improving the content of saponin VI in the cultivated paris polyphylla, which adopts the inoculation of different microorganisms in the process of cultivating the paris polyphylla and simultaneously assists moderate drought treatment, improves the content of the saponin VI and improves the quality of the paris polyphylla; meanwhile, the chlorophyll content of the paris polyphylla seedlings can be improved, and the growth of the paris polyphylla seedlings is promoted.
According to the purpose of the invention, the invention provides the following technical scheme:
a method for improving the content of saponin VI in cultivated Yunnan rhizoma paridis comprises the following steps:
s1 propagation and inoculation of arbuscular mycorrhizal fungi
S101, propagation:
selecting ivory leucocyte enzyme and cryptobiosis sacculus enzyme, mixing humus soil and river sand according to the ratio of 1: 1, setting the autoclave at 121 ℃ for 1h, sterilizing and cooling for later use;
soaking appropriate amount of semen Tritici Aestivi in 0.2% potassium permanganate solution for 15 min;
placing the sterilized mixed sandy soil at 2/3 of a flowerpot, weighing 20g of arbuscular mycorrhizal fungi strains, uniformly scattering the arbuscular mycorrhizal fungi strains in the flowerpot, placing wheat seeds on the arbuscular mycorrhizal fungi strains, and covering the flowerpot with the sandy soil mixture until the flowerpot is full; finally, pouring a proper amount of sterile water;
s102, inoculation:
and (3) mixing the sterilized humus soil and loess according to the weight ratio of 3: 1, uniformly mixing, wherein the inoculation mode of the arbuscular mycorrhizal fungi is that firstly, 40g of arbuscular mycorrhizal fungi weighed in advance are uniformly paved on the soil surface at the position 1/2 in the soil filled in a flowerpot 1/2, then a layer of thin soil is covered, the paris polyphylla seedlings are neatly placed on the soil surface, finally, the whole flowerpot is filled with the soil and is watered sufficiently;
s2, inoculation of growth-promoting bacteria
The growth promoting bacteria strain is simple bacillus obtained by separating Yunnan rhizoma paridis tissue, coating the simple bacillus liquid stored at-20 deg.C on LB plate culture medium, and culturing in 37 deg.C incubator for 24-48 h;
then picking single colony and placing in LB liquid culture medium, setting shaking table at 37 deg.C, 180rpm, shaking for 2-3h to make OD of bacterial liquid be 0.6-0.8, taking out bacterial liquid, 8000rpm/min, centrifuging for 10 min, collecting thallus, adding sterile water, and re-suspending until bacterial concentration is about 2 × 107cfu/ml, and irrigating 50ml of heavy suspension once for each planting of the paris polyphylla seedlings;
s3, daily management
Maintaining the water holding capacity of the soil to be 50%; covering two layers of 9-pin black shading nets on the planting greenhouse, not applying fertilizer to the paris polyphylla seedlings during the treatment period, and watering soil moisture in time according to a weighing method to supplement moisture;
s4, harvesting
After the inoculation treatment and the water control treatment are finished with the paris polyphylla co-culture for 60 days, paris polyphylla seedlings begin to be harvested, potting soil is gently shaken when the seedlings are taken out, and the seedlings are carefully taken out, so that the damage to root systems caused by overlarge actions is avoided.
Further, in S102, the paris polyphylla seedling is a three-year-old paris polyphylla seedling which is developed in root system, has no diseases and damage and grows normally and is used as a seedling.
Further, in S2, LB liquid medium: 10g/L of tryptone, 5g/L of yeast extract and 10g/L of sodium chloride, wherein the volume of distilled water is fixed to 1L, the pH value is adjusted to 7.4, and 15g/L of agar is required to be additionally added for preparing a solid culture medium.
Further, in S101, a flowerpot with a caliber of 21cm and a height of 17cm is selected, washed with clean water, and then sprayed with 75% acetaldehyde for sterilization.
Compared with the prior art, the invention has the beneficial effects that:
the method for improving the content of the saponin VI of the paris polyphylla cultivated by the invention is characterized in that different microorganisms are inoculated in the process of cultivating the paris polyphylla, and moderate drought treatment is assisted, so that the content of the saponin VI is improved, the quality of the paris polyphylla is improved, and meanwhile, the method can improve the chlorophyll content of paris polyphylla seedlings and promote the growth of the paris polyphylla seedlings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is an observation picture of AM infection in Paris polyphylla rhizome;
FIG. 2 is a graph showing hormone content of Paris polyphylla;
FIG. 3 is a N, P, K content chart of Paris polyphylla;
FIG. 4 is a graph showing the saponin content of Paris polyphylla.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for improving the content of saponin VI in cultivated Yunnan rhizoma paridis comprises the following steps:
s1 propagation and inoculation of arbuscular mycorrhizal fungi
S101, propagation:
selecting ivory leucocyte enzyme and cryptobiosis sacculus enzyme, mixing humus soil and river sand according to the ratio of 1: 1, setting the autoclave at 121 ℃ for 1h, sterilizing and cooling for later use;
selecting a flowerpot with the caliber of 21cm and the height of 17cm, washing with clear water, and spraying 75% acetaldehyde for disinfection;
soaking appropriate amount of semen Tritici Aestivi in 0.2% potassium permanganate solution for 15 min;
placing the sterilized mixed sandy soil at 2/3 of a flowerpot, weighing 20g of arbuscular mycorrhizal fungi strains, uniformly scattering the arbuscular mycorrhizal fungi strains in the flowerpot, placing wheat seeds on the arbuscular mycorrhizal fungi strains, and covering the flowerpot with the sandy soil mixture until the flowerpot is full; finally, pouring a proper amount of sterile water;
s102, inoculation:
and (3) mixing the sterilized humus soil and loess according to the weight ratio of 3: 1, uniformly mixing, wherein the inoculation mode of the arbuscular mycorrhizal fungi is that firstly, 40g of arbuscular mycorrhizal fungi weighed in advance are uniformly paved on the soil surface at the position 1/2 in the soil filled in a flowerpot 1/2, then a layer of thin soil is covered, the paris polyphylla seedlings are neatly placed on the soil surface, finally, the whole flowerpot is filled with the soil and is watered sufficiently; the paris polyphylla seedling is a three-year-old paris polyphylla seedling with developed root system, no diseases and no damage and with normal growth as seedling.
S2, inoculation of growth-promoting bacteria
The growth promoting bacteria strain is simple bacillus obtained by separating Yunnan rhizoma paridis tissue, coating the simple bacillus liquid stored at-20 deg.C on LB plate culture medium, and culturing in 37 deg.C incubator for 24-48 h; LB liquid medium: 10g/L of tryptone, 5g/L of yeast extract and 10g/L of sodium chloride, wherein the volume of distilled water is fixed to 1L, the pH value is adjusted to 7.4, and 15g/L of agar is required to be additionally added for preparing a solid culture medium.
Then selecting a single colony and placing the single colony in an LB liquid culture medium, setting a shaking table at 37 ℃, and carrying out shake culture at 180rpm for 2-3h, taking out the bacterial liquid when the OD of the bacterial liquid is 0.6-0.8, centrifuging at 8000rpm/min for 10 min, collecting the thallus, adding sterile water for re-suspending until the bacterial concentration is about 2 multiplied by 107cfu/ml, and irrigating 50ml of re-suspending liquid once for each Yunnan rhizoma paridis seedling subjected to inoculation treatment;
s3, daily management
Maintaining the water holding capacity of the soil to be 50%; covering two layers of 9-pin black shading nets on the planting greenhouse, not applying fertilizer to the paris polyphylla seedlings during the treatment period, and watering soil moisture in time according to a weighing method to supplement moisture;
s4, harvesting
After the inoculation treatment and the water control treatment are finished with the paris polyphylla co-culture for 60 days, paris polyphylla seedlings begin to be harvested, potting soil is gently shaken when the seedlings are taken out, and the seedlings are carefully taken out, so that the damage to root systems caused by overlarge actions is avoided.
Example 2
Control test
The method specifically comprises the following steps:
1. propagation and inoculation of standby mycorrhizal fungi (AM)
Propagation: ivory leukoballoon enzyme (Diclasporia torulosa) and cryptococcoid enzyme (Paraglomus occullum) were selected. Firstly, mixing humus soil and river sand according to the proportion of 1: 1, setting the autoclave at 121 ℃ for 1 hour. Sterilizing and cooling for later use; selecting a flowerpot with the diameter of 21cm and the height of 17cm, washing with clear water, and spraying 75% acetaldehyde for disinfection; soaking appropriate amount of semen Tritici Aestivi in 0.2% potassium permanganate solution for 15 min; placing the sterilized mixed sandy soil at 2/3 of a flowerpot, weighing 20g of AM strain, uniformly spreading the AM strain in the flowerpot, placing wheat seeds on the AM strain, and covering the flowerpot with the sandy soil mixture until the flowerpot is full; finally pouring a proper amount of sterile water.
Inoculation: and (3) uniformly mixing the sterilized humus soil and the loess collected by the later mountains according to the proportion of (3: 1). The inoculation mode of the AM fungi is that firstly, 1/2 soil is filled in a flowerpot, 40g of AM fungi weighed in advance are uniformly paved on the soil surface at the position 1/2, then a layer of thin soil is covered, the paris polyphylla seedlings are neatly paved on the soil surface, finally, the whole flowerpot is filled with soil, and water is poured sufficiently. The whole process of transplanting and inoculating is completed, and the paris polyphylla seedling is a three-year-old paris polyphylla seedling with developed root system, no disease and no damage and normal growth is selected as a seedling.
2. Inoculation with growth-promoting bacteria (PEPR)
The strain is simple Bacillus (Bacillus simplex) separated from Paris polyphylla tissue in the subject group. The bacillus simplex liquid preserved at-20 ℃ is coated on an LB plate culture medium and placed in an incubator at 37 ℃ for 24-48 h. And then picking a single colony to be placed in an LB liquid culture medium: 10g/L of tryptone, 5g/L of yeast extract and 10g/L of sodium chloride, wherein the volume of distilled water is fixed to 1L, the pH value is adjusted to 7.4, and 15g/L of agar is required to be additionally added for preparing a solid culture medium. Shaking table at 37 deg.C and 180rpm for 2-3h to make OD of bacteria liquid 0.6-0.8, taking out bacteria liquid, centrifuging at 8000rpm/min for 10 min, collecting thallus, adding sterile water, and resuspending to bacteria concentration of 2 × 107cfu/ml, and irrigating 50ml of heavy suspension once for each planting of the paris polyphylla seedlings. Sterile water is not inoculated for Comparison (CK), so that the seedlings are prevented from being burnt due to contact with leaves.
Functional identification of AM and PEPR mixed inoculum
Because AM and PEPR have no strict specificity to host plants, the symbiosis of two bacteria in the same plant is a common phenomenon in nature and plays an important role in an ecosystem. However, most studies show that the AM or the endophyte is inoculated to the plants independently, the potential synergistic effect of the combined inoculation is ignored, and the synergistic effect between the two microorganisms is beneficial to improving the growth tolerance of the plants in a stress environment and is also beneficial to relieving the negative effect on the microorganisms in the stress environment.
The plant growth promoting bacteria can promote plant growth by fixing nitrogen, dissolving potassium, dissolving phosphorus, secreting plant hormone, synthesizing siderophore and other substances. The AM fungus infects the plant root system to enlarge the contact area between the root system and the soil, increase the absorption of the plant to the P element, even inhibit some pathogenic bacteria, and generate phytohormone to promote the plant growth. The main interaction mechanism of symbiotic existence of the two in plant root systems is as follows: a. the interaction of the AM and the PGPR promotes the absorption of nutrients by the plants, particularly improves the utilization of N, P by the plants, and thus promotes the growth of the plants; b. the two microorganisms can synergistically inhibit the growth of pathogenic bacteria; c. AM inoculation can synthesize phytohormone to promote plant growth and accumulation of active substances; d. AM hypha continuously grows in soil, so that propagation of surrounding bacteria is promoted, and especially colonization of part of bacteria in the rhizosphere can be increased; e. the bacteria also contribute to the colonization of AM, promoting the growth of AM hyphae and prolonging their life.
4. Differential moisture treatment
Water stress treatment: soil moisture was normal moisture (W): 80% of maximum water capacity in the soil field, and moderate drought treatment (D): the test was controlled at 50% of the field capacity of the soil. Three treatments were set under the same moisture conditions: inoculating 2 kinds of AM fungi and simple bacillus mixed inoculation; AM alone and no inoculation control treatment. There were 6 treatments, each 10 replicates for 60 pots, and the test pots were randomly placed. The water content is controlled by a weighing and water supplementing method, and water is supplemented every two days, so that the soil water content of each paris polyphylla is set within a corresponding control range (directly measured by a water content meter). And recording the weight of each basin in the changed state by using a balance, and weighing the water consumed by each basin and correspondingly replenishing water to each basin when replenishing water for the second time. After the water content is controlled for 90 days, the corresponding index of the paris polyphylla is measured.
5. Daily management
Two layers of 9-pin black shading nets are covered on the planting greenhouse, fertilizer is not applied to Yunnan paris polyphylla seedlings during processing, soil moisture is watered in time according to a weighing method to supplement moisture, and due to the specificity of the test, sterile water cooled by boiled water is watered, a tray is placed at the bottom of the planting pot, so that moisture can not be lost quickly, and cross contamination can not be caused between each treatment. Adjusting the shading rate of the greenhouse suitable for the growth of the paris polyphylla, preventing and treating plant diseases and insect pests, keeping the greenhouse clean and in a good ventilated state, weeding in due time, carrying out potted plant management regularly, and exchanging the sequence of various plant pots in each treatment room and the same treatment room every two weeks.
6. Harvesting
After the inoculation treatment and the water control treatment are finished with the paris polyphylla co-culture for 60 days, paris polyphylla seedlings begin to be harvested, potting soil is gently shaken when the seedlings are taken out, the seedlings are carefully taken out, and the phenomenon that the roots are damaged due to overlarge actions is avoided, so that experimental errors are caused. The following criteria were determined:
firstly, the infection observation of AM in the rhizoma paridis yunnanensis is shown in figure 1:
it was observed that both under normal moisture and moderate drought conditions, arbuscular or vesicular structures were observed in the root system of Paris polyphylla inoculated with the AM fungus, hyphae formed invaded sites from the surface of the root system (shown in FIG. 1A), and hyphae continued to grow inward (shown in FIG. 1B) to form a tree-like structure (shown in FIG. 1C).
II, the hormone content of the paris polyphylla is shown in figure 2:
under moderate drought, the content of ABA and IAA is obviously increased compared with normal water treatment; compared with inoculated AM and CK, the ABA content of the double-inoculated AM + PEPR is respectively increased by 11.55 percent and 23.7 percent; the IAA content is increased by more than one time in the double-inoculated AM + PEPR treatment compared with CK.
Thirdly, N, P, K content of Yunnan rhizoma paridis, as shown in figure 3:
the moderate drought reduces the N, P, K content in the rhizoma paridis yunnanensis, and the treatment of double inoculation AM + PEPR and single inoculation AM has no influence on the N, P content; but shows a significant increase in the content of K. The level of N, P, K was significantly increased compared to CK when treated with AM + PEPR under normal moisture inoculation.
Fourthly, the content of the saponin of the paris polyphylla is shown in figure 4:
in the rhizoma paridis, the content of the rhizoma paridis saponin, whether the rhizoma paridis saponin I, II, VI, VII or the total saponin content is increased under moderate drought compared with the water treatment. The content of the paris saponin VI is found to exist in AM + PEPR treatment under the moisture treatment, and the content of the paris saponin VI is found to be as high as 0.052 percent and 0.06 percent in single inoculation and double inoculation treatment under moderate drought. The moderate drought dual-inoculation treatment is 12.3 percent higher than the inoculation of AM.
The double inoculation of the arbuscular mycorrhizal fungi and the growth-promoting bacteria under moderate drought treatment is beneficial to the accumulation of hormones in the rhizome of the paris polyphylla, is beneficial to the absorption and utilization of the content of K, and promotes the increase of the content of the saponin VI and the increase of total saponins in the paris polyphylla.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (4)
1. A method for improving the content of saponin VI in cultivated Yunnan rhizoma paridis is characterized by comprising the following steps:
s1 propagation and inoculation of arbuscular mycorrhizal fungi
S101, propagation:
selecting ivory leucocyte enzyme and cryptobiosis sacculus enzyme, mixing humus soil and river sand according to the ratio of 1: 1, setting the autoclave at 121 ℃ for 1h, sterilizing and cooling for later use;
soaking appropriate amount of semen Tritici Aestivi in 0.2% potassium permanganate solution for 15 min;
placing the sterilized mixed sandy soil at 2/3 of a flowerpot, weighing 20g of arbuscular mycorrhizal fungi strains, uniformly scattering the arbuscular mycorrhizal fungi strains in the flowerpot, placing wheat seeds on the arbuscular mycorrhizal fungi strains, and covering the flowerpot with the sandy soil mixture until the flowerpot is full; finally, pouring a proper amount of sterile water;
s102, inoculation:
and (3) mixing the sterilized humus soil and loess according to the weight ratio of 3: 1, uniformly mixing, wherein the inoculation mode of the arbuscular mycorrhizal fungi is that firstly, 40g of arbuscular mycorrhizal fungi weighed in advance are uniformly paved on the soil surface at the position 1/2 in the soil filled in a flowerpot 1/2, then a layer of thin soil is covered, the paris polyphylla seedlings are neatly placed on the soil surface, finally, the whole flowerpot is filled with the soil and is watered sufficiently;
s2, inoculation of growth-promoting bacteria
The growth promoting bacteria strain is simple bacillus obtained by separating Yunnan rhizoma paridis tissue, coating the simple bacillus liquid stored at-20 deg.C on LB plate culture medium, and culturing in 37 deg.C incubator for 24-48 h;
then selecting a single colony and placing the single colony in an LB liquid culture medium, setting a shaking table at 37 ℃, and carrying out shake culture at 180rpm for 2-3h, taking out the bacterial liquid when the OD of the bacterial liquid is 0.6-0.8, centrifuging at 8000rpm/min for 10 min, collecting the thallus, adding sterile water for re-suspending until the bacterial concentration is about 2 multiplied by 107cfu/ml, and irrigating 50ml of re-suspending liquid once for each Yunnan rhizoma paridis seedling subjected to inoculation treatment;
s3, daily management
Maintaining the water holding capacity of the soil to be 50%; covering two layers of 9-pin black shading nets on the planting greenhouse, not applying fertilizer to the paris polyphylla seedlings during the treatment period, and watering soil moisture in time according to a weighing method to supplement moisture;
s4, harvesting
After the inoculation treatment and the water control treatment are finished with the paris polyphylla co-culture for 60 days, paris polyphylla seedlings begin to be harvested, potting soil is gently shaken when the seedlings are taken out, and the seedlings are carefully taken out, so that the damage to root systems caused by overlarge actions is avoided.
2. The method for increasing the content of the saponin VI in the cultivated Paris polyphylla according to claim 1, wherein in S102, the seedling of the Paris polyphylla is a seedling of a normal three-year-old Paris polyphylla with a developed root system, no diseases and no damage.
3. The method for increasing the content of the saponin VI of the cultivated paris polyphylla as claimed in claim 1, wherein in S2, an LB liquid culture medium: 10g/L of tryptone, 5g/L of yeast extract and 10g/L of sodium chloride, wherein the volume of distilled water is fixed to 1L, the pH value is adjusted to 7.4, and 15g/L of agar is required to be additionally added for preparing a solid culture medium.
4. The method for increasing the content of saponin VI in cultivated Paris polyphylla according to claim 1, wherein a flowerpot with a caliber of 21cm and a height of 17cm is selected in S101, washed with clean water and then disinfected by spraying with 75% acetaldehyde.
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| CN114793793A (en) * | 2022-05-11 | 2022-07-29 | 甘肃省科学院生物研究所 | Application and method for water saving and yield increase of dry land wheat by synergy of nano elemental iron and arbuscular mycorrhizal fungi |
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