CN112899122A - Closed lateral flow chromatography color development device - Google Patents
Closed lateral flow chromatography color development device Download PDFInfo
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- CN112899122A CN112899122A CN202110229641.4A CN202110229641A CN112899122A CN 112899122 A CN112899122 A CN 112899122A CN 202110229641 A CN202110229641 A CN 202110229641A CN 112899122 A CN112899122 A CN 112899122A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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Abstract
The invention belongs to the technical field of biological detection, and particularly relates to a closed lateral flow chromatography color development device which comprises a shell, a box cover, a reaction tube and a diluent tube, wherein the shell comprises a shell upper layer and a shell lower layer, a blade fixing frame is arranged on the inner side of the shell lower layer, a blade fixing groove is arranged in the blade fixing frame, a blade is arranged on the inner side of the blade fixing groove, a funnel is arranged on the inner side of the shell lower layer, and a test paper strip groove is arranged below the funnel. The reaction tube and the diluent tube in the liquid inlet hole can be forced to move downwards while the box cover is pressed downwards to be closed, the reaction tube and the diluent tube are cut by the blade below, the reaction liquid and the diluent flow out simultaneously, and the reaction liquid and the diluent are mixed in the funnel and then slowly flow into the lateral flow chromatography test strip in the test strip groove to be detected. The device realizes the uncapped and closed nucleic acid detection, overcomes the problem of aerosol pollution, has simple structure, convenient operation and low cost, and is suitable for field and POCT field detection.
Description
Technical Field
The invention belongs to the technical field of biological detection, and particularly relates to a closed lateral flow chromatography color development device.
Background
In recent years, the high-efficiency and accurate nucleic acid detection technology has important application in many fields such as infectious pathogen detection, food safety quarantine, pathogenic gene screening and the like. The current commonly used nucleic acid detection technology is a real-time fluorescence quantitative PCR technology, which has the advantages of high sensitivity and high specificity, but the PCR technology needs repeated heating and cooling processes due to the requirement of thermal cycle, and has higher technical and cost requirements, thereby limiting the application of the technology outside a laboratory. Recently developed isothermal nucleic acid amplification technologies, such as loop-mediated isothermal amplification (LAMP), Recombinase Polymerase Amplification (RPA), Strand Displacement Amplification (SDA), nucleic acid sequence-dependent amplification (NASBA), have the advantages of simplicity and convenience in operation, portability of equipment, high sensitivity, strong specificity, accurate result, rapid detection and the like, and are suitable for field and POCT field detection.
The detection method based on the nucleic acid isothermal amplification product comprises a gel electrophoresis method, a fluorescence method, a turbidity method, a lateral flow chromatography test strip method and the like, but the gel electrophoresis method and the common lateral flow chromatography test strip method need to open a cover and easily cause aerosol pollution; the turbidity method requires visual observation of results and is easily influenced by subjective factors; fluorescence methods require complex equipment and specialized operators. Although many closed, portable and visual nucleic acid detecting apparatuses are available, the construction of these apparatuses is complicated, which is not favorable for cost reduction. Therefore, it is necessary to develop a closed, portable, and simple nucleic acid detecting apparatus for visualization.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a closed lateral flow chromatography color development device.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a closed side flow chromatography color developing device, includes shell, lid, reaction tube, diluent pipe, the shell includes shell upper strata, shell lower floor, the inboard of shell lower floor is provided with the blade mount, establish in the blade mount and be provided with the blade fixed slot, the inboard of blade fixed slot is provided with the blade, the inboard of shell lower floor is provided with the funnel, the below of funnel is provided with test paper strip groove.
Preferably, the shell and the box cover are made of transparent materials, the box cover is of a solid structure, and the left side of the box cover is connected with the left side of the shell; the box cover can be closed by pressing the box cover to the lower right, and the box cover can not be opened after being closed, and the color development result of the lateral flow chromatography test paper can be clearly and conveniently seen by the arrangement that the shell and the box cover are made of transparent materials.
Preferably, the upper layer and the lower layer of the shell are both made of transparent materials, the upper layer of the shell is of a solid structure, two liquid inlet holes are formed in the inner side of the upper layer of the shell, the liquid inlet holes are through holes of a cylindrical structure, and a sealing ring is arranged on an inner ring below each liquid inlet hole; through the setting in two feed liquor holes, conveniently be used for placing reaction tube and diluent pipe, through the setting of sealing washer for ensure that the device is inside sealed, do not produce the contact with external gas.
Preferably, the blade is arranged at a position right below the liquid inlet hole and is vertically arranged with the liquid inlet hole; when the lid is pressed to the below right, the lid produces pressure to reaction tube and diluent pipe gradually, and then makes reaction tube and diluent pipe atress descend, and finally the bottom and the blade of reaction tube and diluent pipe contact each other, and through the setting of blade, the blade is used for cutting the bottom of reaction tube and diluent pipe to make the liquid outflow of reaction tube and diluent pipe the inside.
Preferably, the funnel is arranged at a position right below the blade, the lower end of the funnel extends into the inner side of the test strip groove, and the lateral flow chromatography test strip is fixedly arranged on the inner side of the test strip groove; after the blade cuts the reaction tube and the diluent tube, the liquid in the reaction tube and the diluent tube firstly flows to the funnel to be mixed, and then flows to the test strip groove through the funnel.
Preferably, the lateral flow chromatography test strip comprises a support plate, a sample pad, a conjugate pad, a chromatography membrane and a water absorbent pad.
Preferably, the chromatographic membrane is a nitrocellulose membrane; the nitrocellulose membrane is provided with a quality control line (C line) and a detection line (T line), the C line is coated with biotin, and the T line is coated with a fluorescein antibody; the binding pad contains streptavidin and colloidal gold complex.
The amplified product flows from the sample pad side to the combination pad due to the capillary action force, biotin on the amplified product is specifically combined with streptavidin on the combination pad, after the compound flows to the T line from the sample pad side, fluorescein on the amplified product is specifically combined with a fluorescein antibody on the T line, excessive streptavidin on the combination pad is specifically combined with biotin on the C line, and the streptavidin on the combination pad is marked by colloidal gold, so that if the T line and the C line are simultaneously developed, the reading result is positive; if only C line develops color, the color is negative; if the C line does not develop color, the test strip is invalid.
Compared with the prior art, the invention has the beneficial effects that: the device realizes the closed nucleic acid detection without opening the cover, overcomes the problem of aerosol pollution, and has simple structure, convenient operation and low cost.
The parts of the device not involved are the same as or can be implemented using prior art.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic cross-sectional front view of the present invention;
FIG. 2 is a perspective view of the upper layer of the housing of the present invention;
FIG. 3 is a perspective view of the lower layer of the housing of the present invention;
FIG. 4 is a schematic top view of a blade according to the present invention;
FIG. 5 is a schematic view of the blade holder according to the present invention;
FIG. 6 is a schematic structural diagram of a lateral flow test strip according to the present invention;
FIG. 7 is an enlarged view of A of FIG. 1 according to the present invention;
in the figure: 1. a housing; 11. an upper layer of the housing; 12. a lower layer of the housing; 2. a box cover; 3. a liquid inlet hole; 31. a reaction tube; 32. a diluent tube; 4. a seal ring; 5. a blade; 51. a blade holder; 52. a blade-securing slot; 6. a funnel; 7. a test strip groove; 8. a lateral flow chromatography test strip; 81. a support plate; 82. a sample pad; 83. a bonding pad; 84. a chromatographic membrane; 85. an absorbent pad.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment provides a closed lateral flow chromatography color development device, as shown in fig. 1, including shell 1, lid 2, reaction tube 31, diluent pipe 32, shell 1 includes shell upper strata 11, shell lower floor 12, the inboard of shell lower floor 12 is provided with blade mount 51, establish in blade mount 51 and be provided with blade fixed slot 52, the inboard of blade fixed slot 52 is provided with blade 5, the inboard of shell lower floor 12 is provided with funnel 6, the below of funnel 6 is provided with test paper strip groove 7. The box cover 2 can be closed by pressing the box cover 2 towards the lower right, the box cover 2 cannot be opened after being closed, and the color development result of the lateral flow chromatography test paper 8 can be clearly and conveniently seen by the arrangement that the shell 1 and the box cover 2 are made of transparent materials; the reaction tube 31 and the diluent tube 32 are conveniently placed through the arrangement of the two liquid inlet holes 3, and the sealing ring 4 is arranged to ensure that the interior of the device is sealed and does not contact with the outside gas; when the box cover 2 is pressed towards the lower right, the box cover 2 gradually generates pressure on the reaction tube 31 and the diluent tube 32, so that the reaction tube 31 and the diluent tube 32 are stressed to descend, and finally the bottoms of the reaction tube 31 and the diluent tube 32 are contacted with the blade 5, and through the arrangement of the blade 5, the blade 5 is used for cutting the bottoms of the reaction tube 31 and the diluent tube 32, so that the liquid in the reaction tube 31 and the diluent tube 32 flows out; after the reaction tube 31 and the diluent tube 32 are cut by the blade 5, the liquid in the reaction tube 31 and the diluent tube 32 flows to the funnel 6 for mixing, and then slowly flows to the sample pad 82 of the lateral flow test strip 8 in the test strip groove 7 through the funnel 6 for detection.
The working principle and the using process of the invention are as follows: placing the reaction tube 31 filled with the amplification reaction solution in corresponding equipment for amplification reaction, and taking out the reaction tube 31 after the amplification is finished; then the reaction tube 31 and the diluent tube 32 are respectively placed in the two inlet holes 3 of the lateral flow chromatography color development device of the invention, and the upper box cover 2 is pressed downwards, the reaction tube 31 and the diluent tube 32 are forced to move downwards and are cut by the lower blade 5, the reaction liquid and the diluent simultaneously flow out, are mixed in the funnel 6 and then slowly flow into the sample pad 82 of the lateral flow chromatography test paper 8 in the test paper strip groove 7, and then flow from the sample pad 82 to the combining pad 83 by the capillary force. The results were read through the housing 1 after five minutes: if the T line and the C line are simultaneously developed, judging that the reading result is positive; if only C line develops color, the color is negative; if the C line does not develop color, the test strip is invalid. After detection is finished, the sealed device is directly abandoned at a safe place without being opened.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. An enclosed lateral flow chromatographic development device characterized by: including shell (1), lid (2), reaction tube (31), diluent pipe (32), shell (1) is including shell upper strata (11), shell lower floor (12), the inboard of shell lower floor (12) is provided with blade mount (51), establish in blade mount (51) and be provided with blade fixed slot (52), the inboard of blade fixed slot (52) is provided with blade (5), the inboard of shell lower floor (12) is provided with funnel (6), the below of funnel (6) is provided with test paper strip groove (7).
2. The enclosed lateral flow chromatography color development device of claim 1, characterized in that: the shell (1) and the box cover (2) are made of transparent materials, the box cover (2) is of a solid structure, and the left side of the box cover (2) is connected with the left side of the shell (1).
3. The enclosed lateral flow chromatography color development device of claim 1, characterized in that: shell upper strata (11), shell lower floor (12) are transparent material, and shell upper strata (11) are solid construction, and the inboard of shell upper strata (11) is provided with two feed liquor holes (3), and feed liquor hole (3) are cylindrical structure's through-hole, and the inner circle of every feed liquor hole (3) below all is provided with sealing washer (4).
4. The enclosed lateral flow chromatography color development device of claim 1, characterized in that: the blade (5) is arranged at a position right below the liquid inlet hole (3) and is vertically arranged with the liquid inlet hole (3).
5. The enclosed lateral flow chromatography color development device of claim 1, characterized in that: the funnel (6) is arranged at a position right below the blade (5), the lower end of the funnel (6) penetrates into the inner side of the test strip groove (7), and a lateral flow chromatography test strip (8) is fixedly arranged on the inner side of the test strip groove (7).
6. The enclosed lateral flow chromatography color development device of claim 1, characterized in that: the lateral flow chromatography test strip (8) comprises a support plate (81), a sample pad (82), a binding pad (83), a chromatography membrane (84) and a water absorption pad (85).
Priority Applications (1)
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CN202110229641.4A CN112899122A (en) | 2021-03-02 | 2021-03-02 | Closed lateral flow chromatography color development device |
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CN202110229641.4A CN112899122A (en) | 2021-03-02 | 2021-03-02 | Closed lateral flow chromatography color development device |
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CN202110229641.4A Withdrawn CN112899122A (en) | 2021-03-02 | 2021-03-02 | Closed lateral flow chromatography color development device |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113583862A (en) * | 2021-08-09 | 2021-11-02 | 上海实验动物研究中心 | Pathogen on-site nucleic acid detection device and application method |
CN115521862A (en) * | 2022-09-14 | 2022-12-27 | 大连理工大学 | Nucleic acid detection chip general structure combined with general test strip and use method |
-
2021
- 2021-03-02 CN CN202110229641.4A patent/CN112899122A/en not_active Withdrawn
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113583862A (en) * | 2021-08-09 | 2021-11-02 | 上海实验动物研究中心 | Pathogen on-site nucleic acid detection device and application method |
CN115521862A (en) * | 2022-09-14 | 2022-12-27 | 大连理工大学 | Nucleic acid detection chip general structure combined with general test strip and use method |
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