CN112891380A - Extraction method of giant salamander mucus and grease and pharmaceutical product thereof - Google Patents
Extraction method of giant salamander mucus and grease and pharmaceutical product thereof Download PDFInfo
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Abstract
The invention provides a method for extracting giant salamander mucus and grease and a medicinal product thereof, belonging to the technical field of extraction and preparation of trauma medicaments. The method solves the problems that the existing extraction mode of giant salamander mucus and grease is not good and the medicine preparation efficacy is not enough. The extraction method of giant salamander mucus and grease is simple and comprises the working procedures of fetching, stimulating, scraping giant salamander mucus, killing to obtain giant salamander grease and the like; in the preparation of the medicine, the dried powder of giant salamander mucus is used as a main raw material, giant salamander grease is used as a catalyst, and auxiliary materials such as giant salamander, wild buckwheat, radix tetrastigme and the like are matched to prepare a finished medicine product by a certain process and a certain proportion. Compared with the prior art, the extraction efficiency of giant salamander mucus and grease is high, the giant salamander mucus can be extracted on the premise of not damaging the giant salamander, and secondary extraction is facilitated; and after the extract obtained by the method is prepared into the medicament, the medicament has very obvious curative effect, high pain relieving efficiency and good scar removing effect, and is harmless to human bodies and has no side effect.
Description
Technical Field
The invention belongs to the technical field of extraction and preparation of trauma medicines, and relates to a method for extracting giant salamander mucus and grease and a medicinal product thereof.
Background
Giant salamanders are ancient amphibian animals evolved from 3 hundred million (million) of aquatic fishes in the age of ancient mud pots by 6 million years ago. Mucus secreted from the outer surface of the giant salamander has good medical value and can be used for trauma wounds, burn wounds, scald wounds and surgical operations. In the prior art, improvement needs to be made on how to refine secretions of the giant salamander epidermis, and as giant salamander mucus is a sustainable and renewable resource, periodic refinement can be performed as long as the giant salamander survives, so that the continuity needs to be ensured in a refining mode; furthermore, the comprehensive effects of relieving pain and removing scars of the mucus extracted from the giant salamanders need to be achieved, and further research needs to be carried out.
Disclosure of Invention
The invention aims to provide an extraction method of giant salamander mucus and grease, aiming at the problems that the existing extraction method of giant salamander mucus and grease is poor and the medicine preparation efficacy is deficient.
The purpose of the invention can be realized by the following technical scheme:
a method for extracting giant salamander mucus and grease comprises the following steps:
step (1): taking a live giant salamander, a container capable of containing the whole live giant salamander, a flapping device capable of realizing an impact function, a scraper blade which has the function of scraping the skin of the giant salamander but does not have sharpness enough to scrape the skin of the giant salamander, a liquid collecting container which is provided with a cavity and is filled with a diluted liquid in the cavity, and a solid collecting cavity which is provided with the cavity;
step (2): placing the live giant salamander in a container, impacting the container or the skin of the live giant salamander by using a flapping device to enable the live giant salamander to generate a stress response, so that giant salamander mucus and giant salamander grease are secreted on the skin surface of the live giant salamander;
and (3): scraping giant salamander mucus on the surface of the skin of the giant salamander into a liquid-state collecting container by using a scraper to obtain a finished product A containing giant salamander mucus components, wherein the mass of the giant salamander mucus in each 100ml of diluted solution is 8-15 g; scraping giant salamander mucus into a solid collecting cavity by using a scraper, and drying to obtain a finished product A1 containing giant salamander mucus components and presenting a solid state.
And (4): after the giant salamander is slaughtered, the subcutaneous fat layer of the giant salamander is extracted to obtain a finished product B.
In the above extraction method of giant salamander mucus and grease, the preparation process of the hydrolysate is as follows: pouring 95ml of honey and 5ml of vegetable oil into a beaker, heating the bottom of the beaker, controlling the temperature to be 40-60 ℃, and keeping for 5 minutes to form 100ml of finished product diluted liquid.
A pharmaceutical product of giant salamander mucus and grease extract comprises the following components: radix Damnacanthi powder, wild buckwheat powder, radix Apioris Fortunei powder, and matrix;
the matrix comprises dried powder of finished product A, finished product B, beeswax and honey, wherein: the mass percent of the dried powder of the finished product A is 5-20%, the mass percent of the finished product B is 10-30%, the mass percent of the beeswax is 10-20%, and the balance is honey.
Every 100g of the medicine product contains 2-6 g of Indian damnacanthus herb powder, 3-8 g of wild buckwheat rhizome powder, 5-10 g of radix tetrastigme powder and the balance of matrix powder.
In the pharmaceutical preparation of giant salamander mucus and grease, the giant salamander powder is prepared according to the following process:
step (1): adding a cellulase water solution with the mass percentage concentration of 2-3% into the pulverized damnacanthus indicus powder, standing and stirring for 5-7 hours to obtain a mixed solution D, wherein the mass ratio of the damnacanthus indicus powder to the cellulase water solution is 0.3-1;
step (2): adding ethanol into the mixed solution D to ensure that the content of the ethanol in the mixed solution D is 40-70%;
and (3): and extracting the mixture D, filtering to obtain a filtrate E, concentrating the filtrate E until the specific gravity is 1.5, and performing spray drying to obtain the damnacanthus powder.
In the pharmaceutical product of giant salamander mucus and grease, the wild buckwheat powder is prepared according to the following process:
step (1): adding a cellulase aqueous solution with the mass percentage concentration of 2-4% into the crushed wild buckwheat powder, standing and stirring for 2-3 hours to obtain a mixed solution F, wherein the mass ratio of the wild buckwheat powder to the cellulase aqueous solution is 0.5-1;
step (2): adding ethanol into the mixed solution F to ensure that the content of the ethanol in the mixed solution F is 40-70%;
and (3): extracting the mixture F, filtering to obtain filtrate G, concentrating the filtrate G until the specific gravity is 1.5, and spray drying to obtain wild buckwheat powder.
In the pharmaceutical preparation of giant salamander mucus and grease, the radix tetrastigme powder is prepared according to the following processes:
step (1): adding a cellulase aqueous solution with the mass percentage concentration of 3-5% into the crushed radix tetrastigme wheat powder, standing and stirring for 8-9 hours to obtain a mixed solution H, wherein the mass ratio of the radix tetrastigme powder to the cellulase aqueous solution is 1-2;
step (2): adding ethanol into the mixed solution H to ensure that the content of the ethanol in the mixed solution H is 40-70%;
and (3): and extracting the mixture H, filtering to obtain filtrate I, concentrating the filtrate I until the specific gravity is 2, and performing spray drying to obtain radix tetrastigme powder.
Compared with the prior art, the extraction method of giant salamander mucus and grease has high efficiency and is rapid, the giant salamander mucus can be extracted on the premise of not damaging the giant salamander, and secondary extraction of the giant salamander is facilitated; after the extract obtained by the method is prepared into the medicament, the medicament has very obvious curative effect, high pain relieving efficiency and good scar removing effect, and is harmless to human bodies and has no side effect.
Detailed Description
The following are specific examples of the present invention and further describe the effects of the present invention, but the present invention is not limited to these examples.
Firstly, giant salamander mucus and grease need to be extracted from giant salamander:
step (1): taking a live giant salamander, a container capable of containing the whole live giant salamander, a flapping device capable of realizing an impact function, a scraper blade which has the function of scraping the skin of the giant salamander but does not have sharpness enough to scrape the skin of the giant salamander, a liquid collecting container which is provided with a cavity and is filled with a diluted liquid in the cavity, and a solid collecting cavity which is provided with the cavity;
the method comprises the following steps: the scraper is the best wooden scraper, so that the skin of the giant salamander is not easily damaged, the container is usually a culture pot with a waist-shaped cavity, and the liquid collecting container is provided with a cover to isolate the inside of the liquid collecting container from the outside and avoid oxidation;
step (2): placing the live giant salamander in a container, impacting the container or the skin of the live giant salamander by using a flapping device to enable the live giant salamander to generate a stress response, so that giant salamander mucus and giant salamander grease are secreted on the skin surface of the live giant salamander;
every time the skin of the giant salamander is disturbed, mucus is secreted out of the skin of the giant salamander to enable the body to become very smooth, which is a self-protection mode of the giant salamander, the main component of the mucus is collagen, and the research shows that the content of the giant salamander collagen can reach more than 40-60%, the content is very high, and the molecular structure of the giant salamander collagen is small and is easy to be absorbed by the human body, namely the giant salamander mucus required to be collected in the invention;
and (3): scraping giant salamander mucus on the surface of the skin of the giant salamander into a liquid-state collecting container by using a scraper to obtain a finished product A containing giant salamander mucus components, wherein the mass of the giant salamander mucus in each 100ml of diluted solution is 8-15 g; scraping giant salamander mucus into a solid collecting cavity by using a scraper, and drying to obtain a finished product A1 containing giant salamander mucus components and presenting a solid state.
And (4): after the giant salamander is slaughtered, the subcutaneous fat layer of the giant salamander is extracted to obtain a finished product B.
The preparation process of the diluted liquid comprises the following steps: 95ml of honey and 5ml of vegetable oil are taken and poured into a beaker, the bottom of the beaker is heated and the temperature is controlled at 40 ℃ for 5 minutes, and then 100ml of finished product diluted liquid is formed.
And then, taking the finished product A and the finished product B as raw materials to prepare a pharmaceutical product, wherein the pharmaceutical product mainly comprises Indian damnacanthus herb powder, wild buckwheat rhizome powder, radix tetrastigme powder and a matrix.
Every 100g of the medicine product contains 2-6 g of Indian damnacanthus herb powder, 3-8 g of wild buckwheat rhizome powder, 5-10 g of radix tetrastigme powder and the balance of matrix powder.
The matrix comprises dried powder of finished product A, finished product B, beeswax and honey, wherein: the mass percent of the dried powder of the finished product A is 5-20%, the mass percent of the finished product B is 10-30%, the mass percent of the beeswax is 10-20%, and the balance is honey.
The individual powders of the pharmaceutical preparation are prepared as follows:
preparation of Damnacanthus powder: putting the damnacanthus spicatus into a grinder to be ground to obtain damnacanthus spicatus coarse powder, adding a cellulase aqueous solution with the mass percentage concentration of 2-3% into the damnacanthus spicatus coarse powder, standing and stirring for 5-7 hours to obtain a mixed solution D, wherein the mass ratio of the damnacanthus spicatus powder to the cellulase aqueous solution is 0.3-1; adding ethanol into the mixed solution D to ensure that the content of the ethanol in the mixed solution D is 40-70%; and extracting the mixture D, filtering to obtain a filtrate E, concentrating the filtrate E until the specific gravity is 1.5, and performing spray drying to obtain the damnacanthus powder.
Preparing wild buckwheat powder: putting wild buckwheat into a pulverizer for pulverizing to obtain wild buckwheat coarse powder, adding a cellulase aqueous solution with the mass percentage concentration of 2-4% into the wild buckwheat coarse powder, standing and stirring for 2-3 hours to obtain a mixed solution F, wherein the mass ratio of the wild buckwheat powder to the cellulase aqueous solution is 0.5-1; adding ethanol into the mixed solution F to ensure that the content of the ethanol in the mixed solution F is 40-70%; extracting the mixture F, filtering to obtain filtrate G, concentrating the filtrate G until the specific gravity is 1.5, and spray drying to obtain wild buckwheat powder.
Preparing radix tetrastigme powder: putting radix tetrastigme into a grinder to be ground to obtain coarse radix tetrastigme powder, adding a cellulase aqueous solution with the mass percentage concentration of 3-5% into the radix tetrastigme powder, standing and stirring for 8-9 hours to obtain a mixed solution H, wherein the mass ratio of the radix tetrastigme powder to the cellulase aqueous solution is 1-2; adding ethanol into the mixed solution H to ensure that the content of the ethanol in the mixed solution H is 40-70%; and extracting the mixture H, filtering to obtain filtrate I, concentrating the filtrate I until the specific gravity is 2, and performing spray drying to obtain radix tetrastigme powder.
The Damnacanthus indicus powder has the effects of dispelling wind, promoting diuresis, promoting blood circulation and relieving pain in the pharmaceutical product; the wild buckwheat powder has the effects of clearing away heat and toxic materials, promoting blood circulation, dispelling blood stasis, invigorating spleen, and promoting diuresis; radix Apioris Fortunei powder has effects of clearing heat and detoxicating, promoting blood circulation, resolving hard mass, diminishing inflammation, relieving pain, dispelling pathogenic wind, eliminating phlegm, regulating qi-flowing and invigorating spleen. After the three are prepared together, the pain relieving and inflammation diminishing effects can be achieved on the wound position of a wounded person more quickly, and the growth of external microorganisms is inhibited.
The composition of giant salamander mucus is mainly collagen, can play efficient albumen repair efficiency to patient's wound, giant salamander grease plays the catalytic action wherein more, make the medicine contact each position department of patient's wound with smooth mode, and itself possesses certain repairability, after the wound surface of patient is covered by the anti-inflammatory of high concentration refines thing and the giant salamander mucus extract product of high protein, the wound will separate with the external world, the microorganism is difficult to the invasion, and the human body can voluntarily refine near collagen at the in-process of repairing the wound by itself, make wound repair's efficiency and completeness greatly improve.
The medicine product is finally prepared by adopting matrixes with different proportioning components, the mass proportion content of the damnacanthus powder, the wild buckwheat powder and the radix tetrastigme powder in the medicine product is 5%, the matrix content is 85%, a table is formed after experimental data are carried out on a mouse after II-level burn, and the recovery standard that new hair grows after the burn epidermis scabs and falls off is taken as the result as follows:
table the effect of the drug prepared from the matrix with different component ratios in the experiment of simulating the white mouse
| Oven dried powder of Final product A (%) | Final product B (%) | Beeswax (%) | Honey (%) | Recovery time (sky) | Degree of scarring |
| 5 | 10 | 10 | 75 | 15～18 | Obvious scar |
| 5 | 10 | 15 | 70 | 15～18 | Obvious scar |
| 5 | 10 | 20 | 65 | 15～18 | Obvious scar |
| 10 | 10 | 10 | 70 | 14～16 | Slight scar |
| 15 | 10 | 10 | 65 | 12～14 | No scar left |
| 20 | 10 | 10 | 60 | 12～14 | No scar left |
| 5 | 10 | 15 | 70 | 15～18 | Obvious scar |
| 5 | 20 | 15 | 60 | 15～18 | Obvious scar |
| 5 | 30 | 15 | 50 | 15～18 | Obvious scar |
| 10 | 10 | 10 | 70 | 13～16 | No scar left |
| 10 | 15 | 10 | 65 | 11～13 | No scar left |
| 10 | 30 | 10 | 50 | 11～13 | No scar left |
| 15 | 15 | 15 | 55 | 12～15 | No scar left |
| 15 | 20 | 15 | 50 | 11～12 | No scar left |
| 15 | 30 | 15 | 40 | 11～13 | No scar left |
| 20 | 10 | 20 | 50 | 12～14 | No scar left |
| 20 | 15 | 20 | 45 | 11～12 | No scar left |
| 20 | 20 | 20 | 40 | 11～13 | No scar left |
| 20 | 30 | 20 | 30 | 11～12 | No scar left |
It can be concluded that: beeswax and honey do not occupy the determining factor for the drug effect, which plays the leading factor of the dried powder of the finished product A and the cost B, and the table shows that when the dried powder of the finished product A accounts for 10% by mass and the finished product B accounts for 15% by mass, the drug can achieve very good drug effect, and because giant salamander mucus and grease are very precious resources, the good drug effect can be achieved by adopting the proportion, the cost can be saved to the greatest extent, so that the applicant further suggests adopting the proportion to prepare the drug after the test is finished.
After the applicant adopts the mixture ratio, the medicine is adopted for some wounded persons, and the following data table is finally obtained through statistics according to the description and symptoms of the wounded persons:
the second table shows the application effect of the medicine prepared by adopting 10 percent of the dried powder of the finished product A and 15 percent of the matrix of the finished product B on the wounded
| Personnel | Age (year of old) | Degree of burn | Time to pain (minutes) | Recovery time (sky) | Degree of scarring |
| Wounded person A | 4 | Superficial II grade burn | 30 | 8 | No scar left |
| Wounded person B | 27 | Superficial II grade burn | 20 | 6 | No scar left |
| Wounded person C | 58 | Superficial II grade burn | 35 | 8 | No scar left |
| Wounded person D | 17 | Deep II grade burn | 45 | 10 | No scar left |
| Wounded person E | 35 | Deep II grade burn | 35 | 12 | No scar left |
| Wounded person F | 41 | Deep II grade burn | 45 | 11 | No scar left |
| Wounded G | 24 | Grade III burn | 70 | 19 | No scar left |
| Wounded person H | 52 | Grade III burn | 90 | 22 | Slight scar |
From this table it can be seen that: the medicine prepared by adopting the proportion has the efficacy of comprehensively dealing with burn patients with different degrees.
In the above description: the dried powder of product a may also be replaced by product a 1.
Claims (7)
1. The extraction method of giant salamander mucus and grease is characterized by comprising the following steps:
step (1): taking a live giant salamander, a container capable of containing the whole live giant salamander, a flapping device capable of realizing an impact function, a scraper blade which has the function of scraping the skin of the giant salamander but does not have sharpness enough to scrape the skin of the giant salamander, a liquid collecting container which is provided with a cavity and is filled with a diluted liquid in the cavity, and a solid collecting cavity which is provided with the cavity;
step (2): placing the live giant salamander in a container, impacting the container or the skin of the live giant salamander by using a flapping device to enable the live giant salamander to generate a stress response, so that giant salamander mucus and giant salamander grease are secreted on the skin surface of the live giant salamander;
and (3): scraping giant salamander mucus on the surface of the skin of the giant salamander into a liquid-state collecting container by using a scraper to obtain a finished product A containing giant salamander mucus components, wherein the mass of the giant salamander mucus in each 100ml of diluted solution is 8-15 g; scraping giant salamander mucus into a solid collecting cavity by using a scraper, and drying to obtain a finished product A1 containing giant salamander mucus components and presenting a solid state.
And (4): after the giant salamander is slaughtered, the subcutaneous fat layer of the giant salamander is extracted to obtain a finished product B.
2. The extraction method of giant salamander mucus and grease according to claim 1, wherein the preparation process of the thin hydrolysate is as follows: pouring 95ml of honey and 5ml of vegetable oil into a beaker, heating the bottom of the beaker, controlling the temperature to be 40-60 ℃, and keeping for 5 minutes to form 100ml of finished product diluted liquid.
3. The pharmaceutical preparation of giant salamander mucus and lipid extract of claim 1, comprising the following components: radix Damnacanthi powder, wild buckwheat powder, radix Apioris Fortunei powder, and matrix;
the matrix comprises dried powder of finished product A, finished product B, beeswax and honey, wherein: the mass percent of the dried powder of the finished product A is 5-20%, the mass percent of the finished product B is 10-30%, the mass percent of the beeswax is 10-20%, and the balance is honey.
Every 100g of the medicine product contains 2-6 g of Indian damnacanthus herb powder, 3-8 g of wild buckwheat rhizome powder, 5-10 g of radix tetrastigme powder and the balance of matrix powder.
4. The pharmaceutical preparation of giant salamander mucus and oil according to claim 3, wherein the giant salamander mucus and oil is prepared by the following steps:
step (1): adding a cellulase water solution with the mass percentage concentration of 2-3% into the pulverized damnacanthus indicus powder, standing and stirring for 5-7 hours to obtain a mixed solution D, wherein the mass ratio of the damnacanthus indicus powder to the cellulase water solution is 0.3-1;
step (2): adding ethanol into the mixed solution D to ensure that the content of the ethanol in the mixed solution D is 40-70%;
and (3): and extracting the mixture D, filtering to obtain a filtrate E, concentrating the filtrate E until the specific gravity is 1.5, and performing spray drying to obtain the damnacanthus powder.
5. The pharmaceutical preparation of giant salamander mucus and oil according to claim 3, wherein the wild buckwheat rhizome powder is prepared by the following process:
step (1): adding a cellulase aqueous solution with the mass percentage concentration of 2-4% into the crushed wild buckwheat powder, standing and stirring for 2-3 hours to obtain a mixed solution F, wherein the mass ratio of the wild buckwheat powder to the cellulase aqueous solution is 0.5-1;
step (2): adding ethanol into the mixed solution F to ensure that the content of the ethanol in the mixed solution F is 40-70%;
and (3): extracting the mixture F, filtering to obtain filtrate G, concentrating the filtrate G until the specific gravity is 1.5, and spray drying to obtain wild buckwheat powder.
6. The pharmaceutical preparation of giant salamander mucus and oil according to claim 3, wherein the radix tetrastigme powder is prepared by the following process:
step (1): adding a cellulase aqueous solution with the mass percentage concentration of 3-5% into the crushed radix tetrastigme wheat powder, standing and stirring for 8-9 hours to obtain a mixed solution H, wherein the mass ratio of the radix tetrastigme powder to the cellulase aqueous solution is 1-2;
step (2): adding ethanol into the mixed solution H to ensure that the content of the ethanol in the mixed solution H is 40-70%;
and (3): and extracting the mixture H, filtering to obtain filtrate I, concentrating the filtrate I until the specific gravity is 2, and performing spray drying to obtain radix tetrastigme powder.
7. The pharmaceutical preparation of giant salamander mucus and oil according to claim 3, wherein the dried powder of finished product A is 10% by mass and the dried powder of finished product B is 15% by mass.
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| CN209331132U (en) * | 2018-07-18 | 2019-09-03 | 剑阁县云顶大鲵养殖有限责任公司 | A kind of giant salamander skin mucus extraction element |
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| CN101254246A (en) * | 2008-04-03 | 2008-09-03 | 华中科技大学 | External-used burn and scald treating medicine and its preparation |
| CN104206502A (en) * | 2014-09-15 | 2014-12-17 | 重庆馗旭生物科技股份有限公司 | Slaughtering method comprehensively utilizing giant salamander resources |
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| CN104740678A (en) * | 2015-04-02 | 2015-07-01 | 重庆馗旭生物科技股份有限公司 | Application of giant salamander mucus in preparation of adhesive |
| CN104815349A (en) * | 2015-04-02 | 2015-08-05 | 重庆馗旭生物科技股份有限公司 | Binder prepared from giant salamander mucus and preparation method thereof |
| CN106511399A (en) * | 2016-11-16 | 2017-03-22 | 太鲵生物科技（天津）有限公司 | A use of mucus of andrias davidianus Blanchard |
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| CN112891380B (en) | 2023-03-10 |
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Effective date of registration: 20230420 Address after: 321075 West of Xinfan Reservoir Management Office, Xinfan Township, Tangxi Town, Wucheng District, Jinhua City, Zhejiang Province (self declared) Patentee after: Jinhua Congshan Biotechnology Co.,Ltd. Address before: 321075 Daliyuan village, Xinfan Township, Tangxi Town, Wucheng District, Jinhua City, Zhejiang Province Patentee before: Jinhua Dingxin giant salamander breeding farm |