CN112889839B - Alkaline electrolyzed water synergistic liquid and preparation method and application thereof - Google Patents
Alkaline electrolyzed water synergistic liquid and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/36—Hydrogen production from non-carbon containing sources, e.g. by water electrolysis
Abstract
The invention relates to an alkaline electrolyzed water synergistic solution and a preparation method and application thereof. The alkaline electrolyzed water synergistic fluid comprises alkaline electrolyzed water and a synergistic product, wherein the synergistic product comprises chitosan and/or a chitosan derivative. The preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps: and mixing the alkaline electrolyzed water and the synergist in proportion, and cooling to obtain the alkaline electrolyzed water synergistic solution. In the alkaline electrolyzed water synergistic solution, alkaline electrolyzed water and a synergist are matched with each other, so that the alkaline electrolyzed water synergistic solution has a high-efficiency and long-acting bacteriostatic effect.
Description
Technical Field
The invention belongs to the technical field of daily chemical industry, and relates to an alkaline electrolyzed water synergistic solution as well as a preparation method and application thereof.
Background
The alkaline electrolyzed water is novel functional water, has the characteristics of good bactericidal performance, strong permeability, no residue, no stimulation, no cleaning, safety and no toxicity, is widely applied to the aspects of medicine, agriculture, daily chemicals, food, families and the like, and is particularly suitable for application scenes of wiping and washing.
The sterilization performance of the alkaline electrolyzed water completely depends on the pH value, and the optimal sterilization effect can be achieved by generally maintaining the pH value to be more than 12. However, in practice, alkaline electrolyzed water is susceptible to open conditions (e.g., carbon dioxide in air) and quickly becomes completely ineffective. Research on Wangxiping and the like shows that the pH value of alkaline electrolyzed water changes rapidly due to the surrounding environment and the nature of the alkaline electrolyzed water, and the alkaline electrolyzed water basically fails after being placed for 3 days (Wangxiping, Zhanying, Populan, and the like. preparation research on alkaline electrolyzed water [ J ]. Shandong chemical industry, 2015, 44 (1): 38-39.). Although the alkaline electrolyzed water stored under the sealed condition can be stored for more than one month, the sealed storage condition is difficult to ensure after people start to use in daily household scenes. In other cases with acidic buffers, alkaline electrolyzed water is also almost completely ineffective due to its poor buffering capacity.
In summary, how to provide alkaline electrolyzed water capable of effectively enduring environmental influences and maintain long-acting antibacterial effect has become one of the problems to be solved in the field of alkaline electrolyzed water.
Disclosure of Invention
Aiming at the defects and actual requirements of the prior art, the invention provides the alkaline electrolyzed water synergistic liquid and the preparation method and the application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides an alkaline electrolyzed water synergistic solution comprising alkaline electrolyzed water and a synergist, wherein the synergist comprises chitosan and/or a chitosan derivative.
In the alkaline electrolyzed water synergistic solution, alkaline electrolyzed water can effectively inhibit bacteria; the amino group in the chitosan carries positive charges and is easy to combine with proteins with negative charges on the surface of a bacterial cell membrane, so that the normal physiological functions of bacteria are damaged, and the bacteria die; the chitosan derivative has good water solubility, biocompatibility, degradability and broad-spectrum antibacterial activity, can form a water locking network in aqueous solution, and has moisture retention, film forming property and biological buffering capacity; the alkaline electrolyzed water and the synergist (chitosan and/or chitosan derivative) are matched with each other, so that the buffering capacity of the alkaline electrolyzed water synergist can be improved, and the alkaline electrolyzed water synergist has a high-efficiency and long-acting antibacterial effect.
Preferably, the alkaline electrolyzed water includes alkaline electrolyzed water obtained by electrolysis from any electrolytic solution.
Preferably, the pH of the alkaline electrolyzed water is 10.2 to 12.8, and may be, for example, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 11, 11.1, 11.2, 11.3, 11.4, 11.5, 11.6, 11.7, 11.8, 11.9, 12, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 12.7, or 12.8, and preferably 11.0 to 12.8.
Preferably, the chitosan derivative is a water-soluble chitosan derivative.
Preferably, the chitosan derivative comprises any one of chitosan quaternary ammonium salt, 2-hydroxypropyl trimethyl ammonium chloride chitosan or carboxymethyl chitosan or a combination of at least two of them, wherein typical but non-limiting combinations include: the chitosan quaternary ammonium salt and 2-hydroxypropyl trimethyl ammonium chloride chitosan, the chitosan quaternary ammonium salt and carboxymethyl chitosan or the 2-hydroxypropyl trimethyl ammonium chloride chitosan and carboxymethyl chitosan are preferably chitosan quaternary ammonium salt.
Preferably, the molecular weight of the chitosan and chitosan derivative is each independently 3-100 kDa, for example 3kDa, 5kDa, 7kDa, 10kDa, 15kDa, 20kDa, 25kDa, 30kDa, 35kDa, 40kDa, 45kDa, 50kDa, 55kDa, 60kDa, 65kDa, 70kDa, 75kDa, 80kDa, 85kDa, 90kDa, 95kDa or 100 kDa.
Preferably, the concentration of the potentiator in the alkaline electrolyzed water potentiator solution is 0.1 to 100mg/mL, and may be, for example, 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL, 0.6mg/mL, 0.8mg/mL, 1mg/mL, 2mg/mL, 3mg/mL, 4mg/mL, 5mg/mL, 6mg/mL, 8mg/mL, 10mg/mL, 15mg/mL, 20mg/mL, 25mg/mL, 30mg/mL, 35mg/mL, 40mg/mL, 50mg/mL, 60mg/mL, 70mg/mL, 80mg/mL, 90mg/mL, or 100mg/mL, preferably 1 to 50mg/mL, and more preferably 1 to 20 mg/mL.
In a second aspect, the present invention provides a method for preparing the alkaline electrolyzed water synergistic fluid as described in the first aspect, the method comprising:
and mixing the alkaline electrolyzed water and the synergist in proportion, and cooling to obtain the alkaline electrolyzed water synergistic solution.
Preferably, the mixing temperature is 20 ~ 60 ℃, for example can be 20 ℃, 22 ℃, 23 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃, 45 ℃, 50 ℃, 55 ℃ or 60 ℃, preferably 20 ~ 40 ℃.
Preferably, the mixing time is 0.5 to 12 hours, for example, 0.5 hour, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours and 12 hours, preferably 1 to 6 hours, and more preferably 2 to 4 hours;
preferably, the temperature reduction treatment is carried out to reduce the temperature to 0 to 10 ℃, for example, 0 ℃, 1 ℃, 2 ℃, 3 ℃, 4 ℃, 5 ℃, 6 ℃, 7 ℃, 8 ℃, 9 ℃ or 10 ℃, preferably 3 to 6 ℃.
Preferably, the time of the temperature reduction treatment is 1 to 72 hours, for example, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 12 hours, 18 hours, 20 hours, 24 hours, 48 hours and 72 hours, preferably 6 to 48 hours, and more preferably 12 to 48 hours.
As a preferable technical scheme, the preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
mixing alkaline electrolyzed water and a synergist in proportion at the temperature of 20-60 ℃, and treating for 1-72 hours at the temperature of 0-10 ℃ to obtain the alkaline electrolyzed water synergistic solution.
In a third aspect, the invention provides the use of an alkaline electrolyzed water enhancing solution as defined in the first aspect in the preparation of a disinfectant.
Compared with the prior art, the invention has the following beneficial effects:
(1) in the alkaline electrolyzed water synergistic solution, the alkaline electrolyzed water and a synergist (chitosan and/or a chitosan derivative) are matched with each other, so that the alkaline electrolyzed water synergistic solution has a high-efficiency and long-acting bacteriostatic effect;
(2) the alkaline electrolyzed water synergistic solution has the advantages of simple production process, environmental protection, rich raw material source, low cost and suitability for wide popularization;
(3) the alkaline electrolyzed water synergistic solution has long-acting antibacterial effect, and the antibacterial efficiency reduction rate is lower than 8.4 percent after the synergistic solution is placed for 3 months.
Drawings
FIG. 1 shows the inhibition zones of the alkaline electrolyzed water prepared in example 7 and the alkaline electrolyzed water prepared in comparative example 3 against Escherichia coli and Staphylococcus aureus;
FIG. 2 is a graph showing the bacterial killing rate of the alkaline electrolyzed water enhancing solution prepared in example 4 and the alkaline electrolyzed water of comparative example 4.
Detailed Description
To further illustrate the technical means adopted by the present invention and the effects thereof, the present invention is further described below with reference to the embodiments and the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention.
The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or apparatus used are conventional products commercially available from normal sources, not indicated by the manufacturer.
In the examples of the present invention, alkaline electrolyzed water was prepared according to the literature [ Wangxueping, et al. Research on preparation of strong alkaline electrolyzed water, Shandong chemical engineering, volume 44 of 2015, 38-41] and chitosan, chitosan quaternary ammonium salt, 2-hydroxypropyl trimethyl ammonium chloride chitosan and carboxymethyl chitosan are all purchased from Bailingwei science and technology Limited.
Example 1
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) weighing 10mg of chitosan quaternary ammonium salt with the molecular weight of 10kDa, and adding 100mL of alkaline electrolyzed water (pH 12.0);
(2) sealing, placing on 30 deg.C shaking table, oscillating for 0.5 hr, and dissolving;
(3) after dissolving, standing for 12h at 4 ℃ to obtain the alkaline electrolyzed water synergistic solution.
The pH of the alkaline electrolyzed water synergistic solution was measured by a pH meter, and as a result, the pH was 11.8.
Example 2
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) 10g of 2-hydroxypropyltrimethylammonium chloride chitosan having a molecular weight of 3kDa was weighed out, and 100mL of alkaline electrolyzed water (pH 10.2) was added.
(2) After sealing, the mixture is placed on a shaking table at 20 ℃ and shaken for 12 hours to be dissolved.
(3) After dissolving, standing at 0 ℃ for 72h to obtain the alkaline electrolyzed water synergistic solution.
The pH of the alkaline electrolyzed water synergistic solution was measured by a pH meter, and as a result, the pH was 10.1.
Example 3
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) 100mg of carboxymethyl chitosan with a molecular weight of 20kDa was weighed out, and 100mL of alkaline electrolyzed water (pH 12.0) was added.
(2) After sealing, placing on a shaking table at 40 ℃, shaking for 2h, and dissolving.
(3) After dissolving, standing for 24h at 0 ℃ to obtain the alkaline electrolyzed water synergistic solution.
The pH value of the alkaline electrolyzed water synergistic solution was measured by a pH meter, and as a result, the pH was 12.0.
Example 4
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) 10mg of chitosan quaternary ammonium salt having a molecular weight of 100kDa was weighed, and 100mL of alkaline electrolyzed water (pH 12.8) was added.
(2) After sealing, placing on a shaking table at 60 ℃, shaking for 6h, and dissolving.
(3) After dissolution, standing for 12h at 4 ℃ to obtain the alkaline electrolyzed water synergistic solution.
The pH value of the alkaline electrolyzed water synergistic solution was measured by a pH meter, and as a result, the pH was 12.7.
Example 5
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) 10mg of chitosan quaternary ammonium salt having a molecular weight of 10kDa was weighed, and 100mL of alkaline electrolyzed water (pH 12.0) was added.
(2) After sealing, the mixture is placed on a shaking table at 30 ℃ and shaken for 4 hours to be dissolved.
(3) After dissolving, standing for 12h at 4 ℃ to obtain the alkaline electrolyzed water synergistic solution.
The pH value of the alkaline electrolyzed water synergistic fluid was measured by a pH meter, and as a result, the pH was 12.7.
Example 6
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) 1g of chitosan quaternary ammonium salt having a molecular weight of 30kDa was weighed, and 100mL of alkaline electrolyzed water (pH 11.0) was added.
(2) After sealing, placing on a shaking table at 30 ℃, shaking for 2h, and dissolving.
(3) After dissolving, standing for 12h at 4 ℃ to obtain the alkaline electrolyzed water synergistic solution.
The pH of the alkaline electrolyzed water synergistic solution was measured by a pH meter, and as a result, the pH was 11.0.
Example 7
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) 10mg of hydroxymethyl chitosan with a molecular weight of 20kDa was weighed out and 100mL of alkaline electrolyzed water (pH 12.0) was added.
(2) After sealing, the mixture is placed on a shaking table at 30 ℃ and shaken for 4 hours to be dissolved.
(3) After dissolving, standing for 12h at 4 ℃ to obtain the alkaline electrolyzed water synergistic solution.
The pH of the alkaline electrolyzed water synergistic solution was measured by a pH meter, and as a result, the pH was 11.8.
Example 8
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) 10mg of hydroxymethyl chitosan with a molecular weight of 100kDa was weighed out and 100mL of alkaline electrolyzed water (pH 12.8) was added.
(2) After sealing, the mixture is placed on a shaking table at 20 ℃ and shaken for 6 hours to be dissolved.
(3) After dissolving, standing for 24h at 4 ℃ to obtain the alkaline electrolyzed water synergistic solution.
The pH value of the alkaline electrolyzed water synergistic fluid was measured by a pH meter, and as a result, the pH was 12.7.
Example 9
The embodiment provides an alkaline electrolyzed water synergistic solution, and a preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:
(1) 10mg of chitosan with a molecular weight of 3kDa was weighed out and 100mL of alkaline electrolyzed water (pH 12.8) was added.
(2) After sealing, the mixture is placed on a shaking table at 20 ℃ and shaken for 6 hours to be dissolved.
(3) After dissolving, standing for 1h at 10 ℃ to obtain the alkaline electrolyzed water synergistic solution.
The pH value of the alkaline electrolyzed water synergistic fluid was measured by a pH meter, and as a result, the pH was 12.7.
In order to evaluate the beneficial effects of the alkaline electrolyzed water and the synergist (chitosan and/or chitosan derivative) in a matching way, the alkaline electrolyzed water with different pH values is used as a reference for subsequent antibacterial evaluation and stability evaluation.
Comparative example 1
This comparative example provides an alkaline electrolyzed water having a pH of 10.2.
Comparative example 2
This comparative example provides an alkaline electrolyzed water having a pH of 11.0.
Comparative example 3
This comparative example provides an alkaline electrolyzed water having a pH of 12.0.
Comparative example 4
This comparative example provides an alkaline electrolyzed water having a pH of 12.8.
Test example 1 bacteriostatic effectiveness evaluation test 1
The test example tests the bacteriostatic effect of the samples in each example and comparative example. The test is carried out according to the steps of a quantitative bacteria killing test method in disinfection technical specification (2002 edition) 2.1.1.7, and the test conditions are as follows: action concentration: stock solution, action time: for 30 s. The results of the experiment are shown in table 1.
TABLE 1
As can be seen from table 1, in examples 1 to 9 of the present invention, the alkaline electrolyzed water and the synergist (chitosan and/or chitosan derivative) were used in combination to prepare the alkaline electrolyzed water enhancing solution, and the killing rates of the prepared electrolyzed water enhancing solution on escherichia coli, staphylococcus aureus and candida albicans were all 99.9%, indicating that the alkaline electrolyzed water enhancing solution has a high antibacterial effect; comparative examples 1 to 4 are alkaline electrolyzed water, the pH value of the alkaline electrolyzed water is the same as that of the alkaline electrolyzed water used in each example (for example, example 1 and comparative example 3, example 2 and comparative example 1, example 6 and comparative example 2, or example 8 and comparative example 4), and the killing rate of the alkaline electrolyzed water in comparative examples 1 to 4 on escherichia coli, staphylococcus aureus and candida albicans is lower, which shows that the synergistic effect of the alkaline electrolyzed water and the synergist (chitosan and/or chitosan derivative) is matched for use in the invention, so that the bacteriostatic effect of the synergistic solution of the alkaline electrolyzed water can be obviously improved.
Test example 2 bacteriostatic effectiveness evaluation test 2
The carrier method is adopted to measure the bacteriostatic circle experiment to evaluate the bacteriostatic effect: respectively culturing Escherichia coli 8099 and Staphylococcus aureus ATCC 6538 at 37 deg.C under shaking for 24 hr, and diluting the bacterial liquid to bacterial concentration of 1 × 108CFU/mL, the bacteria were uniformly spread on an agar plate, 60. mu.L of each of the solutions to be tested in examples and comparative examples was added to a well (6 mm diameter) of a pre-perforated agar plate, incubated at 37 ℃ for 24 hours, and the zone diameter was measured, the results are shown in Table 2<Judging that the composition is not inhibitory when the composition is 6mm, marking the composition as (-) and judging that the composition is moderate inhibitory when the diameter of the inhibition zone is 6-8 mm, and inhibitingThe diameter of the bacteria ring is 8-12 mm, the bacteria ring is judged to have strong inhibition (++), and the diameter of the bacteria ring is ≧ 12mm, the bacteria ring is judged to have very strong inhibition (+++).
TABLE 2
As can be seen from Table 2, the alkaline electrolyzed water synergistic solutions prepared in examples 1 to 9 have at least moderate bacteriostatic activity against Escherichia coli and Staphylococcus, and have very strong bacteriostatic activity at most; the electrolyzed water in the comparative examples 1 to 3 has no bacteriostatic effect; the alkaline electrolyzed water in the comparative example 4 is only moderately inhibitory, and the inhibitory activity of the alkaline electrolyzed water is lower than that of the alkaline electrolyzed water synergistic solution prepared in the examples 4 and 8, so that the alkaline electrolyzed water and the synergist (chitosan and/or chitosan derivative) are matched for use, the synergy is realized, and the bacteriostatic effect of the alkaline electrolyzed water synergistic solution can be obviously improved.
The results of the inhibition zone experiment of the alkaline electrolyzed water synergistic solution prepared in example 7 and the alkaline electrolyzed water prepared in comparative example 3 are shown in fig. 1, and it can be seen from fig. 1 that the alkaline electrolyzed water synergistic solution prepared in example 7 has an obvious inhibition zone, while the alkaline electrolyzed water prepared in comparative example 3 has no inhibition zone.
Test example 3 Long-term stability test 1
100mL of the alkaline electrolyzed water synergistic solution prepared in example 4 and the alkaline electrolyzed water prepared in comparative example 4 were taken and left at room temperature, and samples were periodically taken to determine the killing rate against Escherichia coli (the method was the same as in test example 1), and the killing rate was plotted against time to evaluate the storage stability, and the results are shown in FIG. 2.
As can be seen from fig. 2, as the storage time is prolonged, the killing rate of the alkaline electrolyzed water prepared in example 4 is kept stable, while the killing rate of the alkaline electrolyzed water prepared in comparative example 4 is continuously reduced, which indicates that the alkaline electrolyzed water and the synergist (chitosan and/or chitosan derivative) are used in combination to achieve synergistic effect, and the stability of the synergistic solution of the alkaline electrolyzed water can be remarkably improved.
Test example 4 Long-term stability test 2
The alkaline electrolyzed water synergists prepared in examples 1 to 9 and the alkaline electrolyzed water prepared in comparative examples 1 to 4 were left for 3 months, the pH value and the killing rate against Escherichia coli were measured (the same method as in test example 1), and the reduction rate was calculated according to the following formula (1) in comparison with the data at the time of the new preparation.
Decrease (%) - (kill rate (left for 3 months) -kill rate (newly prepared))/kill rate (newly prepared) × 100% (1)
TABLE 3
As can be seen from Table 3, the alkaline electrolyzed water synergistic solution prepared in examples 1-9 has a reduced killing rate of Escherichia coli of less than 8.4% after being placed for 3 months, which indicates that the stability is high and the solution can be stored for a long time; the reduction rate of the killing rate of the alkaline electrolyzed water to the escherichia coli in the comparative examples 1 to 4 is higher than 27 percent; the invention shows that the alkaline electrolyzed water is matched with the synergist (chitosan and/or chitosan derivative) for synergy, and the stability of the alkaline electrolyzed water synergistic solution can be obviously improved.
In conclusion, the alkaline electrolyzed water and the synergist (chitosan and/or chitosan derivative) are matched for use, so that the buffering capacity of the alkaline electrolyzed water synergist can be improved, and the alkaline electrolyzed water synergist has a high-efficiency and long-acting antibacterial effect.
The applicant states that the present invention is illustrated by the above examples to show the detailed method of the present invention, but the present invention is not limited to the above detailed method, that is, it does not mean that the present invention must rely on the above detailed method to be carried out. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (18)
1. An alkaline electrolyzed water synergistic fluid is characterized by comprising alkaline electrolyzed water and a synergist;
the synergist is a chitosan derivative;
the chitosan derivative is any one or the combination of two of 2-hydroxypropyl trimethyl ammonium chloride chitosan or carboxymethyl chitosan;
the pH value of the alkaline electrolyzed water is 10.2-12.8.
2. The alkaline electrolyzed water synergistic solution according to claim 1, wherein the pH value of the alkaline electrolyzed water is 11.0-12.8.
3. The alkaline electrolyzed water synergistic solution of claim 1, wherein the chitosan derivatives have molecular weights of 3 to 100kDa, respectively.
4. The alkaline electrolyzed water synergistic solution as set forth in claim 1, wherein the concentration of the synergist in the alkaline electrolyzed water synergistic solution is 0.1-100 mg/mL.
5. The alkaline electrolyzed water synergistic solution as set forth in claim 4, wherein the concentration of the synergist in the alkaline electrolyzed water synergistic solution is 1-50 mg/mL.
6. The alkaline electrolyzed water synergistic solution as set forth in claim 5, wherein the concentration of the synergist in the alkaline electrolyzed water synergistic solution is 1-20 mg/mL.
7. A method for preparing an alkaline electrolyzed water enhancing solution as defined in any one of claims 1 to 6, comprising:
and mixing the alkaline electrolyzed water and the synergist in proportion, and cooling to obtain the alkaline electrolyzed water synergistic solution.
8. The method according to claim 7, wherein the mixing temperature is 20 to 60 ℃.
9. The method according to claim 8, wherein the mixing temperature is 20 to 40 ℃.
10. The method of claim 9, wherein the mixing time is 0.5 to 12 hours.
11. The method of claim 10, wherein the mixing time is 1 to 6 hours.
12. The method of claim 11, wherein the mixing is carried out for 2 to 4 hours.
13. The preparation method according to claim 7, wherein the temperature reduction treatment is carried out to reduce the temperature to 0-10 ℃.
14. The preparation method according to claim 13, wherein the temperature reduction treatment is temperature reduction to 3-6 ℃.
15. The preparation method according to claim 7, wherein the time of the temperature reduction treatment is 1-72 hours.
16. The preparation method according to claim 15, wherein the time for the temperature reduction treatment is 6-48 hours.
17. The preparation method according to claim 16, wherein the time of the temperature reduction treatment is 12-48 hours.
18. Use of an alkaline electrolyzed water enhancing solution according to any one of claims 1 to 6 for the preparation of a disinfectant.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102422869A (en) * | 2011-11-09 | 2012-04-25 | 上海海洋大学 | Fresh-keeping method for globefish |
| CN106474530A (en) * | 2015-08-24 | 2017-03-08 | 中国科学院金属研究所 | A kind of preparation method of the polyelectrolyte sponge bleeding-stopping dressing based on chitosan oligosaccharide |
| CN105285436A (en) * | 2015-10-26 | 2016-02-03 | 张静 | High-efficiency laying hen feed capable of improving eggshell quality through chitosan oligosaccharide compound amino acid modified inorganic calcium, magnesium and phosphorus, and preparation method of high-efficiency laying hen feed |
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Patent Citations (1)
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|---|---|---|---|---|
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Non-Patent Citations (2)
| Title |
|---|
| 不同pH值碱性电解水杀菌效果评价;邱梅，田少贤，高圆圆，郝智慧;《黑龙江畜牧兽医》;20170830;137 * |
| 甲壳素及其衍生物研究与应用的新进展;杜予民;《应用化学新进展》;20030930;9 * |
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