CN112868919B - Feed for improving quality of ram semen and application thereof - Google Patents
Feed for improving quality of ram semen and application thereof Download PDFInfo
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- CN112868919B CN112868919B CN202110213830.2A CN202110213830A CN112868919B CN 112868919 B CN112868919 B CN 112868919B CN 202110213830 A CN202110213830 A CN 202110213830A CN 112868919 B CN112868919 B CN 112868919B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention particularly provides a feed for improving the quality of ram semen and application thereof. The result of the feeding test shows that the reproductive hormones GnRH, LH, FSH, T and E in the serum and the sperm serum of the ram 2 The content is improved, the contents of antioxidant substances CAT, MDA, SOD, T-A, GSH-PX, NO and ASAFR in ram serum and semen are improved, the sperm density, the number of linear motion sperm and the survival rate in the semen are improved, the death rate and the deformity rate are obviously reduced, and the method has wide practicability and great potential development value in the field of ram breeding.
Description
Technical Field
The invention belongs to the technical field of animal reproductive physiology and reproduction, relates to a feed and application thereof, and particularly relates to the technical field of a feed for improving the sperm quality of rams and application thereof.
Background
The price of mutton is continuously and rapidly increased, the consumption demand is on an accelerated growth trend overall, and the total supply is short of demand. Further accelerating the development of mutton sheep industry. The improvement of the quality of the sheep semen is one of the important measures for enlarging the breeding scale of the mutton sheep in a short period. The quality of the stud ram semen mainly depends on the genetic characteristics, the feeding management and the semen collection technology. Therefore, the stud ram breeding management measures are very important. The quality of the sperm of the ram is poor, and whether the sperm can be used for insemination or not and whether the standard required by fertilization of the ewe can be met or not is determined. The semen quality is related to the production of frozen semen, the conception rate of ewes to be bred and the economic benefit of farmers.
The invention provides a feed for improving the quality of sheep semen, which comprises spinach, rehmannia root, winged euonymus twig, soybean oil, liquorice, wheat bran, black-bone trametes, radix ophiopogonis, yeast powder, honeysuckle, rice bran, bean pulp, shrimp flower, blackberry lily, chinese cabbage, scutellaria baicalensis, salt, lysine, poria cocos, threonine and glucose, wherein the Chinese herbal medicine compound feed is adopted in the existing technical scheme for improving the quality of the ram semen; the invention patent with the application number of 201711245024.3 provides a feed additive for improving breeding capacity and semen quality of male livestock, and raw materials comprise herba epimedii, herba cistanches, eucommia ulmoides, actinolite, vitamins, L-arginine, copper glycinate and pinctada fucata martensii meat; the invention with the application number of 201710733905.3 discloses a preparation method of a feed for improving the quality of sheep semen, which comprises the following steps: spinach, rehmannia glutinosa, euonymus alatus, soybean oil, liquorice, wheat bran, black-bone trametes, radix ophiopogonis, yeast powder, honeysuckle, rice bran, soybean meal, dried small shrimps, blackberry lily, cabbage, scutellaria baicalensis, salt, lysine, poria cocos, threonine and glucose. Although natural plant components are added, the addition of a plurality of Chinese herbal medicine components has certain disadvantages, the problems of high cost and unstable source quality of the Chinese herbal medicine raw materials exist in the aspect of obtaining the first raw materials, the interaction relationship among partial Chinese herbal medicine components is not completely researched, and whether adverse reaction exists or not needs to be further researched.
In some technical solutions, a technical solution of adding a separate additive into a basic ration is adopted, for example, in an invention patent with application number 201811086252.5, a method for improving the quality of animal semen by using Chinese chestnut polysaccharide is provided, male mammals are supplemented with Chinese chestnut polysaccharide by intragastric feeding or adding into feed every day after weaning, the intake dose of small male mammals is 0.1-100mg/kg of body weight, the intake dose of medium male mammals is 0.5-200mg/kg of feed, and in an invention patent with application number 10803530.9, a method for improving the quality of sheep semen is provided, which is characterized in that: the male sheep can take cysteamine by oral taking, and the ratio of the daily dose taken to the weight of the male sheep is 9.0-15.0mg/kg. However, the feed needs large addition amount, the cost is high in actual feeding management, and the economic benefit of cultivation cannot be highlighted.
Disclosure of Invention
Aiming at the defects of complex components, unstable quality control and higher cost in the actual culture process of the technical scheme for improving the quality of the ram semen in the prior art, the invention aims to provide the feed for improving the quality of the ram semen and the application thereof, and the feed is used for breeding hormones GnRH, LH, FSH, T and E in ram serum and the sperm serum 2 The level has obvious influence, and substances CAT, MDA, SOD, T-A and GSH-P with antioxidant capacity in ram serum and refined serum are obviously improved X The contents of NO and ASAFR, ejaculatory amount in semen quality, density, number of dead sperms, death rate, number of malformed sperms, abnormality rate, number of linear motion sperms and survival rate are improved and improved to different degrees, and the method has wide practicability and huge potential development value in the field of ram breeding.
The invention is realized by the following technical scheme:
the invention provides a feed for improving the sperm quality of rams, which is obtained by mixing basic daily ration and L-citrulline, wherein each part of the basic daily ration is prepared by mixing 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 702 100kg of Dabeinong, 186300kg of fattening materials, 13kg of baking soda and 9kg of livestock salt, and the L-citrulline is added according to the proportion of 2-6mg per ram.
Preferably, the addition amount of the L-citrulline is 6mg per pig.
Preferably, the mass content of the basic ration dry matter is 1.26%.
Preferably, the base ration has a moisture content of 41%.
Further, the invention specifically provides application of the feed for improving the quality of the ram semen in the feeding process for improving the quality of the ram semen.
The feeding amount is 0.69kg per pig, and the feeding times are twice per day.
The Dabeinong 702 adopted by the invention is mutton sheep compound feed 702 produced by Xinjiang Dabei farming and animal husbandry science and technology Limited liability company, the fattening feed 186 adopted by the invention is Zhengda-186 mutton sheep concentrated feed produced by Shijiazhuang Zhengda Limited company, and the concentrated feed can be purchased and obtained by common public through public channels.
By implementing the specific invention content of the invention, the following beneficial effects can be achieved:
(1) The feed for improving the quality of the ram semen, which is provided by the invention, is used for improving the quality of the ram semen by adding the basic ration and the L-citrulline in a proper proportion, is low in cost, and the schedule of each sheep is only 2.65 yuan, so that the cost in the breeding process can be obviously reduced, and the feed has wide practicability for the field of ram breeding.
(2) By adopting the feed for improving the quality of the ram semen, provided by the invention, the reproductive hormones GnRH, LH, FSH, T and E in ram serum and the sperm semen can be obviously improved 2 The level of the substances CAT, MDA, SOD, T-A and GSH-P with antioxidant capacity in ram serum and refined serum is obviously improved X NO, ASAFR content.
(3) By adopting the feed for improving the quality of the ram semen, the ejaculatory amount, the density, the number of dead sperm, the death rate, the number of malformed sperm, the abnormality rate, the number of linearly moving sperm and the survival rate of the ram semen quality are improved and improved to different degrees, and the feed has great potential development value for the field of ram breeding.
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Detailed Description
The present invention will be described below by way of examples, but the present invention is not limited to the following examples.
The materials of the invention are: wheat straw, green grass, alfalfa, dabeinong 702, a fertilizer 186, corn, baking soda, animal salt, vitamin AD3, sodium selenite VE, L-citrulline (purchased from Shandong Nuphar Biotech limited company with a purity of 99.1%), "Dabeinong 702" is a mutton sheep compound feed 702 produced by Xinjiang Dabei farming and animal husbandry Limited liability company, and "fattening material 186" adopted by the invention is a concentrated feed of Zhengda-186 mutton sheep produced by Shijiazhuang Zhengda Limited company, distilled water, physiological saline, a blood count plate, a microscope, a disposable blood taking needle, a sodium citrate anticoagulation tube, an antioxidant index in plasma, a plasma hormone level and the like. The reagents and materials can be purchased through public channels, and the equipment and instruments adopted in the process are all common equipment in the field.
All materials, reagents and equipment selected for use in the present invention are well known in the art, but do not limit the practice of the invention, and other reagents and equipment well known in the art may be suitable for use in the practice of the following embodiments of the invention.
The following examples further illustrate the present invention but are not to be construed as limiting thereof. Modifications or substitutions to methods, steps or conditions of the present invention may be made without departing from the spirit and scope of the invention.
The first embodiment is as follows: feed for improving sperm quality of rams
The invention provides a feed for improving the sperm quality of rams, which is obtained by mixing basic daily ration and L-citrulline, wherein each part of the basic daily ration is prepared by mixing 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 702 100kg of Dabeinong, 186 kg of fattening material, 13kg of baking soda and 9kg of livestock salt, and the L-citrulline is added according to the proportion of 2-6mg per ram.
Example two: feed for improving quality of ram semen and application thereof
The invention provides a feed for improving the sperm quality of rams, which is obtained by mixing basic daily ration and L-citrulline, wherein the basic daily ration is obtained by mixing 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 702 100kg of Dabeinong, 186300kg of fattening material, 13kg of baking soda and 9kg of livestock salt, and the L-citrulline is added according to 2 mg/ram; the mass content of the basic ration is 1.26%, and the water content of the basic ration is 41%. The invention provides application of a feed for improving the quality of ram semen in feeding for improving the quality of ram semen, wherein the feeding amount is 0.69kg per ram, and the feeding times are twice per day.
Example three: feed for improving quality of ram semen and application thereof
The invention provides a feed for improving the sperm quality of rams, which is obtained by mixing basic daily ration and L-citrulline, wherein the basic daily ration is obtained by mixing 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 702 100kg of Dabeinong, 186300kg of fattening material, 13kg of baking soda and 9kg of livestock salt, and the L-citrulline is added according to 4 mg/ram; the mass content of the basic ration is 1.26%, and the water content of the basic ration is 41%. The invention provides application of a feed for improving the quality of ram semen in feeding for improving the quality of ram semen, wherein the feeding amount is 0.69kg per ram, and the feeding times are twice per day.
Example four: feed for improving sperm quality of rams and application thereof
The invention provides a feed for improving the sperm quality of rams, which is obtained by mixing basic daily ration and L-citrulline, wherein the basic daily ration is obtained by mixing 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 702 100kg of Dabeinong, 186300kg of fattening material, 13kg of baking soda and 9kg of livestock salt, and the addition amount of the L-citrulline is 6mg per ram; the mass content of the basic ration is 1.26%, and the water content of the basic ration is 41%. The invention provides application of a feed for improving the quality of ram semen in feeding for improving the quality of ram semen, wherein the feeding amount is 0.69kg per ram, and the feeding times are twice per day.
Example five: feed for improving quality of ram semen and application effect thereof
Based on the technical scheme provided by the second embodiment to the fourth embodiment, 32 healthy Turpan black sheep rams in 1 year are selected for training and semen collection, the average weight (62.65 +/-5.79) kg is set, and a control group and test groups I, II and III are set, wherein the test group and the control group are fed with different feeds, and the other feeding management conditions are consistent. In the feeding process, blood samples and semen samples are collected from rams in the colony house in 0, 15, 30, 45 and 60 days respectively, and the contents of reproductive hormone and antioxidant substances in related serum and semen are measured and the semen quality is detected.
1. The effect of feeding different dosages of L-citrulline on the contents of reproductive hormone and antioxidant substances in ram serum and concentrate serum
The measurement results were analyzed by SAS software, and the analysis results are shown in tables 1, 2, 3, 4, and 5 below.
Table 1: effect of feeding different doses of L-citrulline on ram serum reproductive hormone levels
Note: the data in the same row are marked with different lower case letters in shoulder marks to indicate that the difference is obvious (P < 0.05), and marked with the same lower case letters or no letter marks to indicate that the difference is not obvious (P > 0.05); the capital letters in the same row indicate significant difference (P < 0.01), and the same applies below.
Table 2: effect of feeding different doses of L-citrulline on the antioxidant capacity of ram serum
Table 3: effect of feeding different doses of L-citrulline on stud ram seminal emission reproductive hormone levels
Table 4: the effect of feeding different dosages of L-citrulline on the sperm clearance antioxidant capacity of rams
As shown by the analysis results in the tables 1, 2, 3 and 4, through the measurement of the technical levels and the substances with antioxidant capacity in the ram serum and the sperm clearance, the indexes of each test group and the control group are obviously different, and the technical scheme provided by the invention has obvious effect on improving the quality of the ram sperm.
2. Effect of different doses of L-citrulline on ram semen quality
The results are shown in Table 5, and the ejaculatory amount test group III is respectively increased by 4.67%, 4.67% and 7.69% compared with the control group and the test groups I and II, but the difference among the groups is not obvious. Sperm density tests I, II and III are respectively improved by 5.89% (P < 0.01), 7.10% (P < 0.01) and 18.26% (P < 0.01) compared with a control group, and test III is respectively improved by 11.69% (P < 0.01) and 1042% (P < 0.01) compared with test I and test II, and the difference between the test I and the test II is not significant. Compared with the control group, the test I and the test II, the number of dead sperm in the test III group is respectively increased by 10.08 percent (P < 0.01), 8.52 percent (P < 0.01) and 9.47 percent (P < 0.01), and the difference between the control group and the test I and the test II group is not significant. The sperm mortality rate of the control group is respectively improved by 4.56% (P < 0.05), 6.96% (P < 0.01) and 8.43% (P < 0.01) compared with the test groups I, II and III, and the difference among the test groups is not significant. The number of teratospermia and the rate of deformity are not significant in the control group compared with the test groups. Compared with a control group and a test group I and a test group II, the number of the linear motion sperms in the test group III is respectively increased by 21.04 percent (P < 0.01), 12.86 percent (P < 0.01) and 10.66 percent (P < 0.01), the number of the test group I and the test group II is respectively increased by 7.257.10 percent (P < 0.01) and 9.38 percent (P < 0.01) compared with the control group, and the difference between the test group I and the test group II is not obvious. Compared with the control group, the sperm motility rate tests I, II and III respectively improve 1.56% (P > 0.05), 2.29% (P < 0.01) and 2.58% (P < 0.01), and the difference among the test groups is not obvious.
Table 5: effect of different doses of L-citrulline on ram semen quality (n = 8)
As can be seen from the above embodiments, in the technical scheme provided by the invention, the feed for improving the quality of the ram semen, provided by the embodiment four, has the best effect of improving the contents of reproductive hormone and antioxidant substances in ram serum and the ram semen, has the best effect of improving the semen quality index, and has the best feeding effect, namely, the feed adopting 6 mg/L-citrulline added amount combined with basic ration has the best feeding effect.
Through cost measurement, the addition amount of the L-citrulline is a main factor influencing the feeding cost, the cost is also continuous along with the increase of the addition amount of the L-citrulline, after the feeding effect is remarkably improved by adopting the addition amount of 6 mg/L-citrulline in combination with the feed of basic ration, the feeding cost and the feeding effect are comprehensively considered, and the schedule of each sheep is only 2.65 yuan when the addition amount of 6 mg/L-citrulline is adopted, namely the optimal scheme.
The feed for improving the quality of the ram semen, provided by the invention, is used for improving the quality of the ram semen by adding the basic ration and the L-citrulline in a proper proportion, and the feed for improving the quality of the ram semen, obtained by adopting the technical scheme provided by the invention, is low in cost, the schedule of each sheep is only 2.65 yuan, the cost in the breeding process can be obviously reduced, and the feed has wide practicability for the field of ram breeding.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.
Claims (4)
1. The feed for improving the sperm quality of the rams is characterized by being obtained by mixing basic daily ration and L-citrulline, wherein each part of the basic daily ration is prepared by mixing 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 702 100kg of Dabeinong, 186 kg of fattening material, 13kg of baking soda and 9kg of livestock salt, and 6mg of L-citrulline is added per ram; the mass content of the basic ration dry matter is 1.26%.
2. The feed for improving sperm quality in rams according to claim 1 wherein said basal ration has a moisture content of 41%.
3. The use of a feed for improving the sperm quality of rams as claimed in any one of claims 1 to 2 in the preparation of a feed for improving the sperm quality of rams in an amount of 0.69 kg/pig.
4. The use of a feed for improving the sperm quality of a ram as claimed in claim 3, wherein the number of feeds is twice a day.
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