CN106212382A - A kind of method improving sheep semen quality and feedstuff - Google Patents
A kind of method improving sheep semen quality and feedstuff Download PDFInfo
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- CN106212382A CN106212382A CN201610803530.9A CN201610803530A CN106212382A CN 106212382 A CN106212382 A CN 106212382A CN 201610803530 A CN201610803530 A CN 201610803530A CN 106212382 A CN106212382 A CN 106212382A
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- sheep
- cysteamine
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- semen quality
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to herding and animal reproduction field, specifically disclose a kind of method improving sheep semen quality.Present invention firstly discovers that the cysteamine making ram that daily dose 9.0 15.0mg/kg BW is administered orally can significantly improve the motility of sperm of sheep.By the method adding cysteamine in feedstuff, make sheep obtain cysteamine, convenient on-demand interpolation can not only be accomplished, change the dosage of interpolation in time, and be capable of promoting the use of on a large scale of large-scale cultivation sheep.The method of the invention is applied to sheep cultivation, is possible not only to ensure the safety of sheep meat, also will promote the fast development of meat sheep aquaculture simultaneously.
Description
Technical field
The present invention relates to herding and animal reproduction field, relate in particular to a kind of method improving sheep semen quality.
Background technology
People and animals strive grain problem and become increasingly conspicuous, and greatly developing Herbivore is one of national main solution advocated.
Scale, standardization, intensive cultivation situation has been gradually formed at agriculture district sheep husbandry.Along with facility scaleization cultivates not
Disconnected propelling, especially kind sheep sheep hurdle moving region is relatively limited, plants sheep semen quality in the last few years the most on a declining curve, directly
Connect and cause ewe pregnancy rate to present in different regions declining to some extent, and then have impact on apparent availability and the valency of Carnis caprae seu ovis
Lattice.According to industry statistic data, China's Mutton yield 4,410,000 tons in 2015, increase by 2.9% on a year-on-year basis, per capita Carnis caprae seu ovis occupancy volume 3.2 thousand
Gram.But 21 century first arrival 2014, is affected by many factors, Carnis caprae seu ovis rise in price is very fast, most area market supply
Tight slightly, cause society's extensive concern.The main cause that Carnis caprae seu ovis price goes up continuously and healthily is that Carnis caprae seu ovis consumption growth is faster than production increasing
Long, Carnis caprae seu ovis is become national consumption from regionality consumption, minority crowd consumption become whole people's consumption, seasonality consumption become
Perennially consuming, consumption demand is totally in accelerated growth trend, and supply falls short of demand for total amount.Therefore, Sheep development is further speeded up
It it is trend of the times.Agriculture district solves the key of this problem in a short time and expands the cultivation scale of meat sheep exactly, and visits in agriculture district at present
The kind method of sheep semen quality begging for raising facility scaleization cultivation is exactly one of important measure.
Cysteamine (Cysteamine, CS), also known as 2-MEA, is the constituent of coenzyme A, is the life in animal body
Reason active substance, has important physiological action to animal body, and the cysteamine of relatively low-dose typically exhibits antioxygen in vivo
Change effect.CS contains the amino mercapto of reproducibility, has reproducibility.Research shows, CS can induced oxidation reduction sensitive Protein
The expression of Early gowth response factor-1 (Egr-1), and Egr-1 can induce bFGF, PDGF, tissue factor, vascular endothelial growth
The gene expression of factor acceptor.CS can improve temperature capacity, increases the synthesis of heat shock protein.Research finds Oral Administration in Rats CS
After, hypothalamic amicine immunocompetence declines rapidly, and recovers normal after 1 week;CS can make Gaoyou duck reach in advance
Mature weight, feed efficiency increases;CS, in addition to having reduction amicine, improving growth hormone, also can improve
Beta-endorphin, T3, T4, insulin level.The amicine of regulation and control growth hormone release is not only present in central nervous system
Hypothalamus in, there is also in digestive tract.Oral CS then can directly consume gastrointestinal growth inhibited cellulose content, increases growth and swashs
Element and the generation of associated metabolic hormone and release, thus improve the speed of growth of animal.
Summary of the invention
For solving the problem that prior art exists, continuous under the conditions of it is an object of the invention to provide a kind of raising large-scale cultivation
The method of sheep semen quality.
The technical scheme that the inventive method is taked is as follows:
A kind of method improving sheep semen quality, particularly as follows: make ram pass through oral way picked-up cysteamine, picked-up
Daily dose and ram weight ratio be 9.0-15.0mg/kg.
Described cysteamine grain shape is white powder, fusing point 97.0-98.5 DEG C.
Further, sheep absorbs the daily dose of cysteamine and the preferred 12.0mg/kg of weight ratio of sheep.
Further, the 2.5-8.0 monthly age that oral opportunity is ram of described cysteamine.The present invention is ram 2.5
Starting during the monthly age to feed cysteamine, be now sheep Development of Reproductive System very important period, the stage is i.e. the most in early days
Start the growth feeding cysteamine to regulate its reproductive system.
Further, described cysteamine is absorbed by ram by addition in feedstuff.
Further, described sheep is during feeding, and feedstuff is replaced every day, to prevent feed spoilage.
A kind of feedstuff improving sheep semen quality, containing cysteamine, cysteamine in feedstuff proportion according to sheep
Body weight and food ration determine, the total daily dose making cysteamine is 9.0-15.0mg/kg BW.
Further, possibly together with Semen Maydis flour, Sorghum vulgare Pers., wheat bran, bean cake, oil cake of flax seed, corn starch in described feedstuff (concentrate)
Slag (DDGS), alfalfa meal, Petiolus Trachycarpi dregs, yeast powder, conch meal, bone meal, salt, ADE powder, multidimensional, carbamide, trace element, sodium bicarbonate,
One or more in aminoacid, enzyme preparation, choline and phagostimulant, consumption those skilled in the art of said components can be according to often
Rule consumption selects.
Relative to prior art, the beneficial effects of the present invention is:
(1) present invention monitors first and enables sheep oral suitable dose cysteamine to improve sheep seminal fluid product significantly
Matter, and then safely and effectively improve sheep pregnancy rate.Ram picked-up half is made by the way of adding cysteamine in animal feed
Cystamine, can not only accomplish the most on-demand interpolation, changes dosage in time, and can realize on a large scale under the conditions of automatic feeding
Use.
(2) cysteamine that the present invention uses can buied on the market, is applied to improve sheep semen quality, not only may be used
To ensure the safety of sheep meat, additionally it is possible to bring considerable economic benefit and good social benefit.Application the method is permissible
Allow meat sheep scale livestock farming and cultivation self-employed entrepreneurs directly be benefited, improve sheep semen quality, improve sheep raising field and sheep raising is individual
The income at family.
(3) according to nearest China Statistical Yearbook (2015) report, Chinese Sheep breeding stock 1.58 hundred million in 2014.According to
0.8 hundred million adult ewes, pregnancy rate improves 5.0%, often produces one, a tire per year, sells to body weight 30.0kg, and 40.0 yuan/kg counts
Calculating, annual direct economic benefit can improve about 50.0 hundred million yuan, and overall economic benefit is considerable.
Accompanying drawing explanation
The motility of sperm of 8 monthly age sheep during Fig. 1 oral different daily dose cysteamine;
The body weight of 8 monthly age sheep during Fig. 2 oral different daily dose cysteamine;
The AST content of 8 monthly age sheep during Fig. 3 oral different daily dose cysteamine;
The T-SOD content of 8 monthly age sheep during Fig. 4 oral different daily dose cysteamine.
Detailed description of the invention
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.It will be appreciated that following reality
That executes is given merely to play descriptive purpose, is not used to limit the scope of the present invention.The technology of this area
Personnel, in the case of without departing substantially from spirit of the invention and spirit, can carry out various amendment and replacement to the present invention.
Experimental technique used in following embodiment if no special instructions, is conventional method.
The reagent and the material that use in following example are commercial goods.The cysteamine used is from Sigma company
(St.Louis, MO, USA), for white powder, fusing point 97.0-98.5 DEG C.
(1) selection of ram:
Selecting the Small-fat-tail sheep ram at 480 2.5 monthly ages, Small-fat-tail sheep ram is from the limited public affairs of Shandong Kou Shi animal husbandry
Department, is randomly divided into 8 groups (often 60 rams of group) cysteamine in order to various dose of throwing something and feeding by ram.Often group sets 3 repetitions,
20 rams of each repetition, ram can free choice feeding and drinking-water.
(2) rearing conditions of ram:
Fall and winter natural lighting, semi-open sheep hurdle.By 480 rams after the adaptability of 2 weeks is raised, from the
2.5 monthly ages started to add cysteamine in feedstuff, and the daily dose making 8 groups of rams take cysteamine is respectively 0mg/kg (comparison
Group), 3.0mg/kg, 6.0mg/kg, 9.0mg/kg, 12.0mg/kg, 15.0mg/kg, 18.0mg/kg, 21.0mg/kg.Every day three
Secondary replacing feedstuff.
(3) interpretation of result:
Take seminal fluid and the blood of ram when 8 monthly age, seminal fluid uses sperm analysis instrument (Nikon Eclipse
And SCA software detection motility of sperm E200).Blood is carried out glutamic oxaloacetic transaminase, GOT (AST), total number born (T-SOD)
Detection.The data result of above-mentioned analysis is processed: use SPASS20.0 statistical software that data are carried out single factor test variance and divide
Analysis (ANOVA), P < 0.05 thinks that data are that there were significant differences, and P < 0.01 thinks that data difference heteropole is notable.The multiple ratio of average
DuncanShi is relatively used to carry out, result Means ± SEM.
Shown in Fig. 1 for motility of sperm during difference group 8 monthly age of ram, visible in figure, cysteamine daily dose exists
When 9.0mg/kg, 12.0mg/kg, 15.0mg/kg, sheep sperm vigor is significantly higher than matched group, as daily dose 12.0mg/kg
Effect is optimum, and (in figure, a, b, c, d represent the different brackets of motility of sperm: a level shows that motility of sperm is fabulous, and sperm is the most forward
Motion, speed is exceedingly fast;B level shows that motility of sperm is fine, and sperm linearly travels forward, and speed is slower;C level shows motility of sperm
Typically, sperm is curved travels forward;D level shows that motility of sperm is poor, and sperm can only be wriggled in original place.Generally a+b is represented alive
The sperm that power is strong, puts together and shows).
Shown in Fig. 2 for body weight during difference group 8 monthly age of ram, visible in figure, between different groups ram body weight without
Significant difference, it can thus be appreciated that throwing something and feeding of cysteamine does not produce impact to ram body weight.
Shown in Fig. 3 for blood AST during difference group 8 monthly age of ram, visible in figure, ram AST between different groups
Content is without significant difference, it can thus be appreciated that throwing something and feeding of cysteamine does not produce impact to ram AST.
Shown in Fig. 4 for blood T-SOD during difference group 8 monthly age of ram, visible in figure, ram T-between different groups
SOD content is without significant difference, it can thus be appreciated that throwing something and feeding of cysteamine does not produce impact to ram T-SOD.
In summary, feed cysteamine that dosage is 9.0-15.0mg/kg can on certain level sheep semen quality,
Especially when daily dose is 12.0mg/kg, effect is preferable.Therefore the method for the present invention provides one for improving sheep semen quality
Plant beneficial reference.
Although the present invention is described in detail the most with a general description of the specific embodiments in literary composition, but at this
On the basis of bright, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, exist
Without departing from these modifications or improvements on the basis of spirit of the present invention, belong to the scope of protection of present invention.
Claims (8)
1. the method improving sheep semen quality, it is characterised in that particularly as follows: make ram pass through oral way picked-up half
Cystamine, the daily dose of picked-up and ram weight ratio are 9.0-15.0mg/kg.
The method improving sheep semen quality the most according to claim 1, it is characterised in that during described cysteamine oral
Machine is the 2.5-8.0 monthly age of ram.
The method improving sheep semen quality the most according to claim 1 and 2, it is characterised in that sheep picked-up cysteamine
The weight ratio of daily dose and sheep be 12.0mg/kg.
The method improving sheep semen quality the most according to claim 1 and 2, it is characterised in that described cysteamine granule
It is shaped as white powder, fusing point 97.0-98.5 DEG C.
The method improving sheep semen quality the most according to claim 1 and 2, it is characterised in that described cysteamine passes through
Add in feedstuff and absorbed by ram.
The method improving sheep semen quality the most according to claim 5, it is characterised in that described sheep is in the process of nursing
In, feedstuff is replaced every day.
7. the feedstuff improving sheep semen quality, it is characterised in that containing cysteamine, cysteamine is proportion in feedstuff
Determining according to sheep body weight and food ration, the total daily dose making cysteamine is 9.0-15.0mg/kg BW.
The feedstuff improving sheep semen quality the most according to claim 7, it is characterised in that possibly together with jade in described feedstuff
Rice and flour, Sorghum vulgare Pers., wheat bran, bean cake, oil cake of flax seed, corn starch residue, alfalfa meal, Petiolus Trachycarpi dregs, yeast powder, conch meal, bone meal, salt, ADE
One or more in powder, multidimensional, carbamide, trace element, sodium bicarbonate, aminoacid, enzyme preparation, choline and phagostimulant.
Priority Applications (1)
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108904585A (en) * | 2018-09-18 | 2018-11-30 | 青岛农业大学 | A method of animal semen quality is improved using chestnut flower ethyl acetate extract |
CN112868919A (en) * | 2021-02-25 | 2021-06-01 | 新疆农业大学 | Feed for improving quality of ram semen and application thereof |
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CN1649509A (en) * | 2002-02-20 | 2005-08-03 | 华扩达动物科技(I.P.3)有限公司 | Feed for fish and use thereof |
CN101400265A (en) * | 2006-01-20 | 2009-04-01 | 华扩达动物科技(I.P.2)有限公司 | Materials and methods for improving livestock productivity |
CN103445004A (en) * | 2013-05-25 | 2013-12-18 | 安康市汉滨区阳晨牧业科技有限公司 | Feed for promoting estrus of boars |
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CN1649509A (en) * | 2002-02-20 | 2005-08-03 | 华扩达动物科技(I.P.3)有限公司 | Feed for fish and use thereof |
CN101400265A (en) * | 2006-01-20 | 2009-04-01 | 华扩达动物科技(I.P.2)有限公司 | Materials and methods for improving livestock productivity |
CN103445004A (en) * | 2013-05-25 | 2013-12-18 | 安康市汉滨区阳晨牧业科技有限公司 | Feed for promoting estrus of boars |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108904585A (en) * | 2018-09-18 | 2018-11-30 | 青岛农业大学 | A method of animal semen quality is improved using chestnut flower ethyl acetate extract |
CN108904585B (en) * | 2018-09-18 | 2021-05-28 | 青岛农业大学 | Method for improving animal semen quality by using chestnut flower ethyl acetate extract |
CN112868919A (en) * | 2021-02-25 | 2021-06-01 | 新疆农业大学 | Feed for improving quality of ram semen and application thereof |
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