CN106212382A - A kind of method improving sheep semen quality and feedstuff - Google Patents

A kind of method improving sheep semen quality and feedstuff Download PDF

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Publication number
CN106212382A
CN106212382A CN201610803530.9A CN201610803530A CN106212382A CN 106212382 A CN106212382 A CN 106212382A CN 201610803530 A CN201610803530 A CN 201610803530A CN 106212382 A CN106212382 A CN 106212382A
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CN
China
Prior art keywords
sheep
cysteamine
feedstuff
semen quality
ram
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Pending
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CN201610803530.9A
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Chinese (zh)
Inventor
闵令江
赵勇
王彦迪
寇鑫
初美强
崔连涛
于帅
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Qingdao Agricultural University
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Qingdao Agricultural University
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Priority to CN201610803530.9A priority Critical patent/CN106212382A/en
Publication of CN106212382A publication Critical patent/CN106212382A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02Breeding vertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to herding and animal reproduction field, specifically disclose a kind of method improving sheep semen quality.Present invention firstly discovers that the cysteamine making ram that daily dose 9.0 15.0mg/kg BW is administered orally can significantly improve the motility of sperm of sheep.By the method adding cysteamine in feedstuff, make sheep obtain cysteamine, convenient on-demand interpolation can not only be accomplished, change the dosage of interpolation in time, and be capable of promoting the use of on a large scale of large-scale cultivation sheep.The method of the invention is applied to sheep cultivation, is possible not only to ensure the safety of sheep meat, also will promote the fast development of meat sheep aquaculture simultaneously.

Description

A kind of method improving sheep semen quality and feedstuff
Technical field
The present invention relates to herding and animal reproduction field, relate in particular to a kind of method improving sheep semen quality.
Background technology
People and animals strive grain problem and become increasingly conspicuous, and greatly developing Herbivore is one of national main solution advocated. Scale, standardization, intensive cultivation situation has been gradually formed at agriculture district sheep husbandry.Along with facility scaleization cultivates not Disconnected propelling, especially kind sheep sheep hurdle moving region is relatively limited, plants sheep semen quality in the last few years the most on a declining curve, directly Connect and cause ewe pregnancy rate to present in different regions declining to some extent, and then have impact on apparent availability and the valency of Carnis caprae seu ovis Lattice.According to industry statistic data, China's Mutton yield 4,410,000 tons in 2015, increase by 2.9% on a year-on-year basis, per capita Carnis caprae seu ovis occupancy volume 3.2 thousand Gram.But 21 century first arrival 2014, is affected by many factors, Carnis caprae seu ovis rise in price is very fast, most area market supply Tight slightly, cause society's extensive concern.The main cause that Carnis caprae seu ovis price goes up continuously and healthily is that Carnis caprae seu ovis consumption growth is faster than production increasing Long, Carnis caprae seu ovis is become national consumption from regionality consumption, minority crowd consumption become whole people's consumption, seasonality consumption become Perennially consuming, consumption demand is totally in accelerated growth trend, and supply falls short of demand for total amount.Therefore, Sheep development is further speeded up It it is trend of the times.Agriculture district solves the key of this problem in a short time and expands the cultivation scale of meat sheep exactly, and visits in agriculture district at present The kind method of sheep semen quality begging for raising facility scaleization cultivation is exactly one of important measure.
Cysteamine (Cysteamine, CS), also known as 2-MEA, is the constituent of coenzyme A, is the life in animal body Reason active substance, has important physiological action to animal body, and the cysteamine of relatively low-dose typically exhibits antioxygen in vivo Change effect.CS contains the amino mercapto of reproducibility, has reproducibility.Research shows, CS can induced oxidation reduction sensitive Protein The expression of Early gowth response factor-1 (Egr-1), and Egr-1 can induce bFGF, PDGF, tissue factor, vascular endothelial growth The gene expression of factor acceptor.CS can improve temperature capacity, increases the synthesis of heat shock protein.Research finds Oral Administration in Rats CS After, hypothalamic amicine immunocompetence declines rapidly, and recovers normal after 1 week;CS can make Gaoyou duck reach in advance Mature weight, feed efficiency increases;CS, in addition to having reduction amicine, improving growth hormone, also can improve Beta-endorphin, T3, T4, insulin level.The amicine of regulation and control growth hormone release is not only present in central nervous system Hypothalamus in, there is also in digestive tract.Oral CS then can directly consume gastrointestinal growth inhibited cellulose content, increases growth and swashs Element and the generation of associated metabolic hormone and release, thus improve the speed of growth of animal.
Summary of the invention
For solving the problem that prior art exists, continuous under the conditions of it is an object of the invention to provide a kind of raising large-scale cultivation The method of sheep semen quality.
The technical scheme that the inventive method is taked is as follows:
A kind of method improving sheep semen quality, particularly as follows: make ram pass through oral way picked-up cysteamine, picked-up Daily dose and ram weight ratio be 9.0-15.0mg/kg.
Described cysteamine grain shape is white powder, fusing point 97.0-98.5 DEG C.
Further, sheep absorbs the daily dose of cysteamine and the preferred 12.0mg/kg of weight ratio of sheep.
Further, the 2.5-8.0 monthly age that oral opportunity is ram of described cysteamine.The present invention is ram 2.5 Starting during the monthly age to feed cysteamine, be now sheep Development of Reproductive System very important period, the stage is i.e. the most in early days Start the growth feeding cysteamine to regulate its reproductive system.
Further, described cysteamine is absorbed by ram by addition in feedstuff.
Further, described sheep is during feeding, and feedstuff is replaced every day, to prevent feed spoilage.
A kind of feedstuff improving sheep semen quality, containing cysteamine, cysteamine in feedstuff proportion according to sheep Body weight and food ration determine, the total daily dose making cysteamine is 9.0-15.0mg/kg BW.
Further, possibly together with Semen Maydis flour, Sorghum vulgare Pers., wheat bran, bean cake, oil cake of flax seed, corn starch in described feedstuff (concentrate) Slag (DDGS), alfalfa meal, Petiolus Trachycarpi dregs, yeast powder, conch meal, bone meal, salt, ADE powder, multidimensional, carbamide, trace element, sodium bicarbonate, One or more in aminoacid, enzyme preparation, choline and phagostimulant, consumption those skilled in the art of said components can be according to often Rule consumption selects.
Relative to prior art, the beneficial effects of the present invention is:
(1) present invention monitors first and enables sheep oral suitable dose cysteamine to improve sheep seminal fluid product significantly Matter, and then safely and effectively improve sheep pregnancy rate.Ram picked-up half is made by the way of adding cysteamine in animal feed Cystamine, can not only accomplish the most on-demand interpolation, changes dosage in time, and can realize on a large scale under the conditions of automatic feeding Use.
(2) cysteamine that the present invention uses can buied on the market, is applied to improve sheep semen quality, not only may be used To ensure the safety of sheep meat, additionally it is possible to bring considerable economic benefit and good social benefit.Application the method is permissible Allow meat sheep scale livestock farming and cultivation self-employed entrepreneurs directly be benefited, improve sheep semen quality, improve sheep raising field and sheep raising is individual The income at family.
(3) according to nearest China Statistical Yearbook (2015) report, Chinese Sheep breeding stock 1.58 hundred million in 2014.According to 0.8 hundred million adult ewes, pregnancy rate improves 5.0%, often produces one, a tire per year, sells to body weight 30.0kg, and 40.0 yuan/kg counts Calculating, annual direct economic benefit can improve about 50.0 hundred million yuan, and overall economic benefit is considerable.
Accompanying drawing explanation
The motility of sperm of 8 monthly age sheep during Fig. 1 oral different daily dose cysteamine;
The body weight of 8 monthly age sheep during Fig. 2 oral different daily dose cysteamine;
The AST content of 8 monthly age sheep during Fig. 3 oral different daily dose cysteamine;
The T-SOD content of 8 monthly age sheep during Fig. 4 oral different daily dose cysteamine.
Detailed description of the invention
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.It will be appreciated that following reality That executes is given merely to play descriptive purpose, is not used to limit the scope of the present invention.The technology of this area Personnel, in the case of without departing substantially from spirit of the invention and spirit, can carry out various amendment and replacement to the present invention.
Experimental technique used in following embodiment if no special instructions, is conventional method.
The reagent and the material that use in following example are commercial goods.The cysteamine used is from Sigma company (St.Louis, MO, USA), for white powder, fusing point 97.0-98.5 DEG C.
(1) selection of ram:
Selecting the Small-fat-tail sheep ram at 480 2.5 monthly ages, Small-fat-tail sheep ram is from the limited public affairs of Shandong Kou Shi animal husbandry Department, is randomly divided into 8 groups (often 60 rams of group) cysteamine in order to various dose of throwing something and feeding by ram.Often group sets 3 repetitions, 20 rams of each repetition, ram can free choice feeding and drinking-water.
(2) rearing conditions of ram:
Fall and winter natural lighting, semi-open sheep hurdle.By 480 rams after the adaptability of 2 weeks is raised, from the 2.5 monthly ages started to add cysteamine in feedstuff, and the daily dose making 8 groups of rams take cysteamine is respectively 0mg/kg (comparison Group), 3.0mg/kg, 6.0mg/kg, 9.0mg/kg, 12.0mg/kg, 15.0mg/kg, 18.0mg/kg, 21.0mg/kg.Every day three Secondary replacing feedstuff.
(3) interpretation of result:
Take seminal fluid and the blood of ram when 8 monthly age, seminal fluid uses sperm analysis instrument (Nikon Eclipse And SCA software detection motility of sperm E200).Blood is carried out glutamic oxaloacetic transaminase, GOT (AST), total number born (T-SOD) Detection.The data result of above-mentioned analysis is processed: use SPASS20.0 statistical software that data are carried out single factor test variance and divide Analysis (ANOVA), P < 0.05 thinks that data are that there were significant differences, and P < 0.01 thinks that data difference heteropole is notable.The multiple ratio of average DuncanShi is relatively used to carry out, result Means ± SEM.
Shown in Fig. 1 for motility of sperm during difference group 8 monthly age of ram, visible in figure, cysteamine daily dose exists When 9.0mg/kg, 12.0mg/kg, 15.0mg/kg, sheep sperm vigor is significantly higher than matched group, as daily dose 12.0mg/kg Effect is optimum, and (in figure, a, b, c, d represent the different brackets of motility of sperm: a level shows that motility of sperm is fabulous, and sperm is the most forward Motion, speed is exceedingly fast;B level shows that motility of sperm is fine, and sperm linearly travels forward, and speed is slower;C level shows motility of sperm Typically, sperm is curved travels forward;D level shows that motility of sperm is poor, and sperm can only be wriggled in original place.Generally a+b is represented alive The sperm that power is strong, puts together and shows).
Shown in Fig. 2 for body weight during difference group 8 monthly age of ram, visible in figure, between different groups ram body weight without Significant difference, it can thus be appreciated that throwing something and feeding of cysteamine does not produce impact to ram body weight.
Shown in Fig. 3 for blood AST during difference group 8 monthly age of ram, visible in figure, ram AST between different groups Content is without significant difference, it can thus be appreciated that throwing something and feeding of cysteamine does not produce impact to ram AST.
Shown in Fig. 4 for blood T-SOD during difference group 8 monthly age of ram, visible in figure, ram T-between different groups SOD content is without significant difference, it can thus be appreciated that throwing something and feeding of cysteamine does not produce impact to ram T-SOD.
In summary, feed cysteamine that dosage is 9.0-15.0mg/kg can on certain level sheep semen quality, Especially when daily dose is 12.0mg/kg, effect is preferable.Therefore the method for the present invention provides one for improving sheep semen quality Plant beneficial reference.
Although the present invention is described in detail the most with a general description of the specific embodiments in literary composition, but at this On the basis of bright, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, exist Without departing from these modifications or improvements on the basis of spirit of the present invention, belong to the scope of protection of present invention.

Claims (8)

1. the method improving sheep semen quality, it is characterised in that particularly as follows: make ram pass through oral way picked-up half Cystamine, the daily dose of picked-up and ram weight ratio are 9.0-15.0mg/kg.
The method improving sheep semen quality the most according to claim 1, it is characterised in that during described cysteamine oral Machine is the 2.5-8.0 monthly age of ram.
The method improving sheep semen quality the most according to claim 1 and 2, it is characterised in that sheep picked-up cysteamine The weight ratio of daily dose and sheep be 12.0mg/kg.
The method improving sheep semen quality the most according to claim 1 and 2, it is characterised in that described cysteamine granule It is shaped as white powder, fusing point 97.0-98.5 DEG C.
The method improving sheep semen quality the most according to claim 1 and 2, it is characterised in that described cysteamine passes through Add in feedstuff and absorbed by ram.
The method improving sheep semen quality the most according to claim 5, it is characterised in that described sheep is in the process of nursing In, feedstuff is replaced every day.
7. the feedstuff improving sheep semen quality, it is characterised in that containing cysteamine, cysteamine is proportion in feedstuff Determining according to sheep body weight and food ration, the total daily dose making cysteamine is 9.0-15.0mg/kg BW.
The feedstuff improving sheep semen quality the most according to claim 7, it is characterised in that possibly together with jade in described feedstuff Rice and flour, Sorghum vulgare Pers., wheat bran, bean cake, oil cake of flax seed, corn starch residue, alfalfa meal, Petiolus Trachycarpi dregs, yeast powder, conch meal, bone meal, salt, ADE One or more in powder, multidimensional, carbamide, trace element, sodium bicarbonate, aminoacid, enzyme preparation, choline and phagostimulant.
CN201610803530.9A 2016-09-06 2016-09-06 A kind of method improving sheep semen quality and feedstuff Pending CN106212382A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108904585A (en) * 2018-09-18 2018-11-30 青岛农业大学 A method of animal semen quality is improved using chestnut flower ethyl acetate extract
CN112868919A (en) * 2021-02-25 2021-06-01 新疆农业大学 Feed for improving quality of ram semen and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1649509A (en) * 2002-02-20 2005-08-03 华扩达动物科技(I.P.3)有限公司 Feed for fish and use thereof
CN101400265A (en) * 2006-01-20 2009-04-01 华扩达动物科技(I.P.2)有限公司 Materials and methods for improving livestock productivity
CN103445004A (en) * 2013-05-25 2013-12-18 安康市汉滨区阳晨牧业科技有限公司 Feed for promoting estrus of boars

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1649509A (en) * 2002-02-20 2005-08-03 华扩达动物科技(I.P.3)有限公司 Feed for fish and use thereof
CN101400265A (en) * 2006-01-20 2009-04-01 华扩达动物科技(I.P.2)有限公司 Materials and methods for improving livestock productivity
CN103445004A (en) * 2013-05-25 2013-12-18 安康市汉滨区阳晨牧业科技有限公司 Feed for promoting estrus of boars

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108904585A (en) * 2018-09-18 2018-11-30 青岛农业大学 A method of animal semen quality is improved using chestnut flower ethyl acetate extract
CN108904585B (en) * 2018-09-18 2021-05-28 青岛农业大学 Method for improving animal semen quality by using chestnut flower ethyl acetate extract
CN112868919A (en) * 2021-02-25 2021-06-01 新疆农业大学 Feed for improving quality of ram semen and application thereof

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Application publication date: 20161214