CN109392829A - A kind of method for breeding and feed improving bull semen quantity and quality - Google Patents
A kind of method for breeding and feed improving bull semen quantity and quality Download PDFInfo
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- CN109392829A CN109392829A CN201811173164.9A CN201811173164A CN109392829A CN 109392829 A CN109392829 A CN 109392829A CN 201811173164 A CN201811173164 A CN 201811173164A CN 109392829 A CN109392829 A CN 109392829A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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Abstract
The invention belongs to farm animals breeding technology fields, and in particular to a kind of method for breeding and feed for improving bull semen quantity and quality.The method for breeding of the raising bull semen quantity and quality is the following steps are included: adding fresh " double low " cabbage type rape in the basal diet of breeding oxen is feed.The present invention adds fresh rape feeding breeding oxen in basal diet, it can get higher former smart yield and freeze smart yield, and the index of quality such as sperm concentration, bacterial population, Frozen semen activity, abnormal rate, forward motile sperm number are also relatively high, it is suitable with addition carrot feeding effect.In addition, vigor will be significantly higher than carrot group before the jelly of rape group bull semen.Therefore it can be added in basal diet with fresh " double low " cabbage type rape substitution carrot and feed breeding oxen, improve the quantity and quality of bull semen.
Description
Technical field
The invention belongs to farm animals breeding technology fields, and in particular to a kind of raising bull semen quantity and quality
Method for breeding and feed.
Background technique
Freezing of semen not only can be with long-term preservation excellent genes, can be with batch production, large-scale production, at low cost, benefit
It is high.Artificial insemination is carried out using frozen semen, efficiency can be more than the manyfold of dam natural mating breeding head number, such as Niu Keda
Hundreds times or more, significantly improve the breeding efficiency of excellent sire, it is therefore prevented that the transmission between male and female poultry accelerates house
The breeding improvement of poultry and breeding process, the technology play an important role to promotion Animal husbandry production to modernization development.Currently,
How much all closely related with the quality of frozen semen success or failure, dam conception rate and the litter size of artificial insemination be, and frozen semen product
The core index of matter is made of semen volume, sperm concentration, sperm motility and sperm morphology etc..
The feeding sire such as vitamin A, vitamin C, vitamin E and beta carotene is added in basal diet, can be mentioned
Height freezes fine work matter, additional income.Vitamin E be so far prove with the most close vitamin of animal reproduction property relationship it
One.It secretes promoting sexual gland hormone by anterior pituitary, adjusts formation and activity that sexual performance promotes sperm.Vitamin E deficiency can
Cause male animal organ of multiplication impaired, such as spermatogonium is degenerated, and supplements appropriate vitamin E in daily ration, sire sperm can be improved
Quantity, Fresh semen activity reduce abnormal rate and increase sperm motility after defrosting.Vitamin C can prevent the peroxidation of frozen sperm
It answers, improves defrosting sperm motility, extend the sperm in vitro time-to-live.Vitamin A plays a significant role in regulation proliferation, and
Beta carotene is main provitamin A, can reduce livestock lipid peroxide quantity and improve body antioxidant status, day
Beta carotene is added in grain can be improved breeding oxen production essence amount, fresh smart vigor, sperm concentration and Frozen semen activity.Some researches show that,
Vitamin C, vitamin E and content beta-carotene in carrot is higher, therefore in freezing essence production, frequently on basis
A certain amount of carrot feeding sire is added in daily ration, then essence is frozen in collecting semen, production, and it is remarkably improved and freezes fine work matter,
Increase the income of sire farm.
Summary of the invention
To solve the deficiencies in the prior art, the present invention provides a kind of raising sides for improving bull semen quantity and quality
Method and feed.
Technical solution provided by the present invention is as follows:
A kind of method for breeding improving bull semen quantity and quality, comprising the following steps: in the basic day of breeding oxen
The fresh rape of " double low " Wild cabbage type is added in grain.
Based on the above-mentioned technical proposal, fresh rape feeding breeding oxen is added in basal diet, can get higher former essence
Yield and the smart yield of jelly, and the index of quality such as sperm concentration, bacterial population, Frozen semen activity, abnormal rate, forward motile sperm number also compare
It is higher, it is suitable with addition carrot feeding effect.In addition, vigor will be significantly higher than carrot before the jelly of rape group bull semen
Group.Therefore it can be added in basal diet with fresh " double low " cabbage type rape substitution carrot and feed breeding oxen, improve kind
The quantity and quality of bull semen.Low-sulfur glycosides and low erucic acid cabbage type rape are double low cabbage type rapes, refer to mustard in rape oil
Acid content is lower than 3%, and glucosinolate content is lower than the rape variety of 30 micromoles per gram cakes in rapeseed cake.
Specifically the additive amount of the fresh rape of " double low " Wild cabbage type is 8~12kg/ days.
Based on above-mentioned additive amount, adds fresh rape and feed breeding oxen into basal diet, breeding oxen can be significantly improved
The quantity and quality of sperm.
Preferably, the additive amount of fresh rape is 10kg/ days.
Based on above-mentioned additive amount, adds fresh rape and feed breeding oxen into basal diet, breeding oxen can be significantly improved
The quantity and quality of sperm.
The present invention also provides a kind of feed for improving bull semen quantity and quality, each group including following parts by weight
Point: 3~6 parts of basal diet and 1~3 part of fresh rape.
Fresh rape feeding breeding oxen is added in basal diet, can get higher former smart yield and freezes smart yield, and
The index of quality such as sperm concentration, bacterial population, Frozen semen activity, abnormal rate, forward motile sperm number are also relatively high, with addition carrot
Feeding effect is suitable.In addition, vigor will be significantly higher than carrot group before the jelly of rape group bull semen.It therefore can be with fresh
" double low " cabbage type rape substitution carrot, which is added in basal diet, feeds breeding oxen, improves the quantity and matter of bull semen
Amount.
Preferably, the feed of raising bull semen quantity and quality includes each component of following parts by weight: 3~5 parts
Basal diet and 1~2 part of fresh rape.
Based on above-mentioned additive amount, adds fresh rape and feed breeding oxen into basal diet, breeding oxen can be significantly improved
The quantity and quality of sperm.
Generally, fresh rape feeding breeding oxen is added in basal diet, can get higher former smart yield and freezes essence
Yield, and the index of quality such as sperm concentration, bacterial population, Frozen semen activity, abnormal rate, forward motile sperm number are also relatively high, and adds
Add carrot feeding effect suitable.In addition, vigor will be significantly higher than carrot group before the jelly of rape group bull semen.It therefore can
Breeding oxen is fed to be added in basal diet with fresh " double low " cabbage type rape substitution carrot, improves bull semen
Quantity and quality.
Specific embodiment
The principles and features of the present invention are described below, and illustrated embodiment is served only for explaining the present invention, is not intended to
It limits the scope of the invention.
Embodiment
1, the fresh rapeseed cultivation of " double low " Wild cabbage type
Idle 9 mu of the field of Breeding bull station is chosen in this test, sows " double low " wild cabbage after site preparation was turned on September 20th, 2017
Type rape raises oily No. 2 rape varieties, other planting technologies are produced with normal rapeseed.In mid-December, 2017, Brassica campestris L seedling are up to
50~60 centimetres, green forage yield reaches 4 tons or more per acre.
2, bull semen quality determination
Experimental animal: healthy and sexually matured male kind of ox totally 20.
Basal feed: ox mixed concentrate, sheep's hay.
Test equipment: semen collection cylinder, microscope, water-bath, sperm concentration analyzer, blood cell counting plate, pipettor etc. are used
Measure semen quality index.
3, experimental design
This test period is 7 months.
First stage: before 2017.12.30, to feed early period.All 20 to participate in the experiment breeding oxens are all made of identical normal
Advise the mode raising of daily ration；
Second stage: 2018.1.1 to 2018.4.30 is feed period, by breeding oxen 20 participated in the experiment by date of birth, life
It produces performance and is divided into two groups, wherein one group using the feeding method for adding carrot (every ox 5kg/ days) in basal diet, in addition
One group using the feeding method for adding fresh rape (every ox 10kg/ days) in basal diet.
Phase III: 2018.5.1-2018.6.1 is the feeding later period.Two groups of breeding oxens be simultaneously stopped addition carrot with
Fresh rape, only feeds basal diet.
Respectively in the first stage, second stage, phase III taken at regular intervals bull semen, measure the crucial index of quality.
4, testing index and method
Volume of semen: record statistics acquires the actual quantity of fresh essence every time, and unit is milliliter.Freeze essence amount: record statistics is each
The actual count of essence is frozen in production, and unit is branch.Fresh precision: it is carried out using French Etablissements Caillau (IMV) sperm concentration instrument.Fresh essence
The detection of vigor and Frozen semen activity: 10 grades of marking systems are used, detect the sperm count linearly moved under the microscope.Teratospermia
Rate: breeding oxen rate of teratosperm is detected according to the requirement of GB4143-2008 " cow frozen semen national standard ".Forward travel
Sperm count: it is in the sperm count of forward travel in every dosage sperm, is counted with blood counting chamber.Bacterial population: every dosage sperm
The bacteria colony count observed after cultivation carries out Bacteria Culture with culture dish.
5, result and analysis
The influence of fresh rape and carrot to the bull semen index of quality is added in 5.1 basal diets
The influence of fresh rape and carrot to the bull semen index of quality is added in basal diet is shown in Table 1.Test knot
Fruit shows (to add fresh rape and carrot not add fresh rape and carrot before feeding to check the number) in feeding and raise
The smart yield of original, the smart yield of jelly of (fresh rape and carrot are added in stopping) kind ox, forward motile sperm number are significantly high after feeding
Before feeding (P < 0.05), vigor, abnormal rate are substantially less than and (P < 0.05) before feeding before freezing；Frozen semen activity in feeding is significant
Higher than before feeding and after feeding (P < 0.05)；Bacterial population in feeding is significantly higher than before feeding and after feeding (P < 0.05)；Sperm
Density be not significantly different (p > 0.05) in each phase.
The above result shows that adding in basal diet compared with not adding fresh rape and carrot in basal diet
Former smart yield can be significantly improved, freeze the qualities such as smart yield, forward motile sperm number and abnormal rate by adding fresh rape and carrot
Index；Stop after adding fresh rape and carrot, before each index of quality is also higher than addition.Illustrate, is added in basal diet
Fresh rape and carrot can significantly improve each semen quality index of breeding oxen, and this added with apparent Carry-over effect.
Table 1 adds the influence of fresh rape and carrot to different times bull semen quality
Different feeding periods compare, and data shoulder mark capitalization difference indicates significant difference (P < 0.05), identical to indicate poor
Different not significant (p > 0.05)；
Compare between different feeds, data shoulder mark lowercase difference indicates significant difference (P < 0.05), identical expression difference
Not significant (p > 0.05).
Fresh rape and carrot are added in 5.2 basal diets, and carrot is added to the comparison in difference of bull semen quality
And influence of the fresh rape during feeding to bull semen quality is shown in Table 2.The result shows that the density of kind ox sperm, jelly
Vigor, bacterial population are not significantly different (p > 0.05) between rape group and carrot group afterwards, and former smart yield, freeze smart yield,
Vigor, abnormal rate and forward motile sperm number have significant difference (P < 0.05) between each group before freezing.Wherein, just former smart yield and
Speech, carrot group are significantly higher than rape group (P < 0.05)；On freezing smart yield, there is no significance difference between carrot group and rape group
Different (p > 0.05)；Before jelly on vigor, carrot group and rape group are without significant difference (p > 0.05)；In the index of abnormal rate,
(p > 0.05) is not significantly different between rape group and carrot group；On forward motile sperm number, rape group and carrot group
Between without significant difference (p > 0.05).
The above result shows that after adding fresh rape or carrot feeding breeding oxen in basal diet, in addition to rape group
The smart yield of the original of bull semen is substantially less than carrot, and the two difference for freezing smart yield is not significant；And rape group kind is public
Vigor will be significantly higher than carrot group before the jelly of ox sperm.In addition, sperm concentration, bacterial population, Frozen semen activity, abnormal rate, advance
Difference of the index of quality such as motile number between the two is not significant.This illustrates to freeze in essence production, can with it is a certain amount of newly
Fresh rape replaces carrot.
The comparison of 2 rape group of table and carrot group bull semen quality
In table between each data, the different expression significant difference (p < 0.05) of shoulder mark English alphabet, the identical table of English alphabet
Show that difference is not significant (p > 0.05).
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (5)
1. a kind of method for breeding for improving bull semen quantity and quality, which comprises the following steps: in breeding oxen
Basal diet in add double low fresh rapes of Wild cabbage type.
2. the method for breeding according to claim 1 for improving bull semen quantity and quality, it is characterised in that: double low sweet
The additive amount of the blue fresh rape of type is 8~12kg/ days.
3. the method for breeding according to claim 2 for improving bull semen quantity and quality, it is characterised in that: double low sweet
The additive amount of the blue fresh rape of type is 10kg/ days.
4. a kind of feed for improving bull semen quantity and quality, which is characterized in that each component including following parts by weight: 3
~6 parts of basal diet and 1~3 part of double low fresh rapes of Wild cabbage type.
5. the feed according to claim 4 for improving bull semen quantity and quality, which is characterized in that including following heavy
Measure each component of part: 3~5 parts of basal diet and 1~2 part of double low fresh rapes of Wild cabbage type.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811173164.9A CN109392829B (en) | 2018-10-09 | 2018-10-09 | Breeding method and feed for improving quantity and quality of seminal fluid of breeding bull |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811173164.9A CN109392829B (en) | 2018-10-09 | 2018-10-09 | Breeding method and feed for improving quantity and quality of seminal fluid of breeding bull |
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| Publication Number | Publication Date |
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| CN109392829A true CN109392829A (en) | 2019-03-01 |
| CN109392829B CN109392829B (en) | 2022-05-17 |
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| CN201811173164.9A Active CN109392829B (en) | 2018-10-09 | 2018-10-09 | Breeding method and feed for improving quantity and quality of seminal fluid of breeding bull |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113106159A (en) * | 2020-01-09 | 2021-07-13 | 华中农业大学 | Genetic marker for evaluating bovine semen quality and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113106159A (en) * | 2020-01-09 | 2021-07-13 | 华中农业大学 | Genetic marker for evaluating bovine semen quality and application thereof |
| CN113106159B (en) * | 2020-01-09 | 2022-05-06 | 华中农业大学 | Genetic marker for evaluating bovine semen quality and application thereof |
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| CN109392829B (en) | 2022-05-17 |
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