CN112868672A - Bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof - Google Patents
Bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof Download PDFInfo
- Publication number
- CN112868672A CN112868672A CN202110115748.6A CN202110115748A CN112868672A CN 112868672 A CN112868672 A CN 112868672A CN 202110115748 A CN202110115748 A CN 202110115748A CN 112868672 A CN112868672 A CN 112868672A
- Authority
- CN
- China
- Prior art keywords
- bacillus thuringiensis
- suspending agent
- meloidogyne incognita
- stock solution
- aluminum silicate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
- A01N63/23—B. thuringiensis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a bacillus thuringiensis suspending agent for killing meloidogyne incognita and an application thereof, wherein the suspending agent comprises the following components in parts by weight: 93.5-96.2 of Bacillus thuringiensis fermentation stock solution, 0.3-0.5 of potassium sorbate, 0.5-1.0 of magnesium aluminum silicate, 3-5 of calcium lignosulfonate and 4.5-5.5 of pH value; the invention also provides a preparation method and application of the suspending agent, and the suspending agent has a good insecticidal effect on Meloidogyne incognita, is a high-efficiency biological pesticide, and has good application and popularization prospects.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof.
Background
At present, more than 3000 nematodes are harmful to plants, and more than 40 nematodes are found in China, wherein one of the nematodes which are most harmful to crops is root-knot nematode. The root-knot nematode has tiny bodies, lives in the plant body or soil, can be transferred along with rainwater, labor operation and the transfer of diseased plants, and is extremely difficult to control. It is not fully estimated that nematodes cause economic losses around $ 1500 billion globally, and that in our country losses due to nematodes are about $ 35 billion per year. In tropical and subtropical areas with serious root-knot nematode diseases, the yield of crops is reduced by over 90 percent. Root knot nematode disease must be prevented effectively by strictly implementing a plant protection policy of prevention-oriented and comprehensive control, emphasizing on grasping agricultural and physical control measures and matching with chemical and biological control. However, the varieties of the wire killer products available in the market are few, a few products such as abamectin and fosthiazate have become absolute major for nematode prevention and treatment, and the products have been continuously used for decades from the market to the present, so that a lot of nematodes have already generated serious drug resistance, and the new varieties are urgently needed to be replaced. Biological nematicides such as paecilomyces lilacinus and verticillium pachycetes can enter the market in recent years, but compared with chemical nematicides, the varieties are too small in quantity, and novel biological nematicides are urgently needed to be innovated.
Bacillus thuringiensis (Bacillus thuringiensis) is a gram-positive agrobacterium belonging to the genus Bacillus, and its thallus is short rod-shaped, flagellated, single-grown or short-chain. It can produce several insecticidal crystal proteins in the course of sporulation, and has higher insecticidal activity for several pests of lepidoptera, diptera, hymenoptera, coleoptera and nematode, so that it is currently the most extensively-used biological insecticide product in the world.
The bacillus thuringiensis NBIN-863 is a strain disclosed by the applicant and has patent rights, and the preservation number is CCTCC NO: m2013612, and the strain can be used for controlling meloidogyne incognita.
The invention mainly aims at NBIN-863 bacterial strain to create and apply different dosage forms, and the market capacity and application effect of functional microorganism products such as Bacillus thuringiensis (Bt) and the like are closely related to the dosage forms. Hitherto, domestic Bt formulations comprise wettable powder, suspending agent, oil suspending agent, water dispersible granules and the like, and different formulations are suitable for different plants, different medication modes or different pests. The suspending agent is a water-based liquid pesticide preparation, is generally prepared by carrying out wet superfine grinding on a bacillus original drug or fermentation liquor, a surfactant such as a dispersing agent, a viscosity regulator, an antifreezing agent and the like by taking water as a medium, and has a viscous and flowable liquid appearance. The suspending agent has the advantages of fine active ingredient particles, generally 1-5 microns in particle size, good dispersibility and spreadability, high suspension rate, firm adhesion to the surface of a plant, rain wash resistance and high drug effect. It has the features of both missible oil and wettable powder and has no flammability and chemical damage caused by organic solvent. The suspending agent can be uniformly mixed and dispersed with water in any proportion, is convenient to use, does not add an organic solvent in the processing process, basically has no three-waste discharge in the production process, has simple production process, is not easy to pollute the environment, has high yield and high safety, and becomes a main formulation of biological pesticide.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the bacillus thuringiensis suspension for killing the meloidogyne incognita, which is used for preventing and treating the meloidogyne incognita and has more friendly ecological benefit to human bodies and environmental organisms.
The invention also aims to provide application of the bacillus thuringiensis suspending agent for killing meloidogyne incognita.
In order to solve the technical problems, the invention adopts the following technical scheme:
the bacillus thuringiensis suspending agent for killing meloidogyne incognita comprises the following components in parts by weight: 93.5-96.2 of Bacillus thuringiensis fermentation stock solution, 0.3-0.5 of potassium sorbate, 0.5-1.0 of magnesium aluminum silicate, 3-5 of calcium lignosulfonate and 4.5-5.5 of pH value; the Bacillus thuringiensis is Bacillus thuringiensis NBIN-863, and the preservation number is CCTCC NO: m2013612.
The effective bacteria concentration of the bacillus thuringiensis in the suspending agent is 52-112 multiplied by 108CFU/mL；
The Bacillus thuringiensis fermentation stock solution can be obtained according to the conventional fermentation method of the Bacillus thuringiensis.
The above-mentioned suspending agent, preferably, the components of the suspending agent according to the parts by weight ratio include: 93.75-95.85 of Bacillus thuringiensis fermentation stock solution, 0.4-0.5 of potassium sorbate, 0.5-1.0 of magnesium aluminum silicate, 3-5 of calcium lignosulfonate and 4.5-5.5 of pH value;
the above-mentioned suspending agent, preferably, the components of the suspending agent according to the parts by weight ratio include: 93.75-95.00 of Bacillus thuringiensis fermentation stock solution, 0.4-0.5 of potassium sorbate, 0.5-1.0 of magnesium aluminum silicate, 43-5 of calcium lignosulfonate and 4.5-5.5 of pH value;
the application of the bacillus thuringiensis suspending agent for killing the meloidogyne incognita to control the meloidogyne incognita comprises preparing the suspending agent into a pesticide for the meloidogyne incognita, in particular to field killing of the meloidogyne incognita such as tomatoes, cucumbers, lettuce, radishes and the like; can also be used for killing caenorhabditis elegans.
Compared with the prior art, the invention has the following technical effects:
the suspending agent is the first creation of bacillus thuringiensis on the dosage form of a biological nematicide product, has more obvious effect of preventing and treating target nematodes and is safer for users and environmental organisms compared with the conventional medicament. The invention can achieve the ecological benefit of more effectively preventing and controlling the meloidogyne incognita and being more friendly to human bodies and environmental organisms. The field control effect test result shows that the compound pesticide has excellent insecticidal activity on meloidogyne incognita and can be popularized and used as an effective pesticide for field control.
The invention provides a special Bacillus thuringiensis NBIN-863 and a suspending agent formula for biological control of meloidogyne incognita and caenorhabditis elegans.
Drawings
FIG. 1 is a graph of the biological control effect of tomato root-knot nematode;
wherein, A, clear water control group; NBIN-863 Biogroup; C. avermectin, fosthiazate chemical group; D. plant root condition of the clear water control group; plant root status of NBIN-863 Biogroup; F. abamectin, fosthiazate chemical group.
FIG. 2 is a diagram of the biological control effect of cucumber root-knot nematode;
wherein, A, clear water control group; NBIN-863 Biogroup; C. fosthiazate chemical group.
FIG. 3 is a chart of the biological control effect of meloidogyne raphanus.
FIG. 4 is a graph of the biological control effect of lettuce root knot nematodes.
Detailed Description
The technical scheme of the invention is a conventional operation method in the field if not specifically stated, and the reagent or the material is sourced from commercial sources if not specifically stated.
The Bacillus thuringiensis (Bacillus thuringiensis) provided by the embodiment of the invention is Bacillus thuringiensis NBIN-863, and the preservation number is CCTCC NO: m2013612, disclosed in patent document No. 201410137036.4.
The preparation method of the Bacillus thuringiensis fermentation stock solution comprises the following steps:
the fermentation medium is prepared from 3.52% of starch, 3.47% of soybean meal, 0.4% of yeast powder, 0.3% of industrial peptone, pH7.0 and the balance of water.
The fermentation was carried out in a 200L fermenter (120L pot volume) with a ratio of aeration per minute to fermenter volume of 1.5: 1, the culture temperature is 30 ℃, the inoculation amount of the Bacillus thuringiensis NBIN-863 seed liquid is 1 percent, the rotating speed is 300r/min, and the fermentation time is 36 hours.
After fermentation is completed, the effect in the fermentation stock solutionThe bacterial concentration is 79X 108CFU/mL, used in the following examples.
Example 1:
preparing a bacillus thuringiensis suspending agent for killing meloidogyne incognita:
863 suspending agent 1:
94.7g of Bacillus thuringiensis fermentation stock solution, 0.3g of potassium sorbate, 1.0g of magnesium aluminum silicate and 4.0g of calcium lignosulfonate, and the pH value is adjusted to 4.5 by dilute hydrochloric acid.
Adding the components into Bacillus thuringiensis fermentation stock solution, and stirring.
863 suspending agent 2:
94.95g of Bacillus thuringiensis fermentation stock solution, 0.3g of potassium sorbate, 0.75g of magnesium aluminum silicate and 4.0g of calcium lignosulfonate, and the pH value is adjusted to 5.0 by dilute hydrochloric acid.
863 suspending agent 3:
93.7g of Bacillus thuringiensis fermentation stock solution, 0.3g of potassium sorbate, 1.0g of magnesium aluminum silicate and 5.0g of calcium lignosulphonate, and the pH value is adjusted to 5.5 by dilute hydrochloric acid.
863 suspending agent 4:
94.1g of Bacillus thuringiensis fermentation stock solution, 0.4g of potassium sorbate, 0.5g of magnesium aluminum silicate and 5.0g of calcium lignosulfonate, and the pH value is adjusted to 5.0 by dilute hydrochloric acid.
863 suspending agent 5:
95.85g of Bacillus thuringiensis fermentation stock solution, 0.4g of potassium sorbate, 0.75g of magnesium aluminum silicate and 3.0g of calcium lignosulphonate, and the pH value is adjusted to 5.5 by dilute hydrochloric acid.
863 suspending agent 6:
94.6g of Bacillus thuringiensis fermentation stock solution, 0.4g of potassium sorbate, 1.0g of magnesium aluminum silicate and 4.0g of calcium lignosulfonate, and the pH value is adjusted to 4.5 by dilute hydrochloric acid.
863 suspending agent 7:
95.0g of Bacillus thuringiensis fermentation stock solution, 0.5g of potassium sorbate, 0.5g of magnesium aluminum silicate and 4.0g of calcium lignosulfonate, and the pH value is adjusted to 5.5 by dilute hydrochloric acid.
863 suspending agent 8:
93.75g of Bacillus thuringiensis fermentation stock solution, 0.5g of potassium sorbate, 0.75g of magnesium aluminum silicate and 5.0g of calcium lignosulfonate, and the pH value is adjusted to 4.5 by dilute hydrochloric acid.
863 suspending agent 9:
95.5g of Bacillus thuringiensis fermentation stock solution, 0.5g of potassium sorbate, 1.0g of magnesium aluminum silicate and 3.0g of calcium lignosulphonate, and the pH value is adjusted to 5.0 by dilute hydrochloric acid.
Example 2:
preparation of control suspension:
1) fermentation stock solution water dilution: directly diluting the stock solution of the Bacillus thuringiensis fermentation with water to make the effective bacteria concentration same as that of the 863 suspending agent 6;
2) adjuvant replacement control 1:
94.6g of Bacillus thuringiensis fermentation stock solution, 0.4g of sodium benzoate, 1.0g of magnesium aluminum silicate and 4.0g of calcium lignosulphonate, and the pH value is adjusted to 4.5 by dilute hydrochloric acid.
3) Adjuvant replacement control 2:
94.6g of Bacillus thuringiensis fermentation stock solution, 0.4g of potassium sorbate, 1.0g of xanthan gum and 4.0g of calcium lignosulfonate, and the pH value is adjusted to 4.5 by dilute hydrochloric acid.
Example 3:
indoor activity determination of the southern root knot nematode killing bacillus thuringiensis suspending agent on caenorhabditis elegans:
LC50(mg/L) calculated procedure: bacillus thuringiensis suspending agents (prepared in example 1) and a control (prepared in example 2) are added into a 96-well plate according to 5 different concentration gradients (1000mg/L, 100mg/L, 10mg/L, 1mg/L and 0.1mg/L), about 40 nematodes are added into each well, the total system for life test is 200 mu L, purified water is used as a control, each group is repeated for 3 times, the action is performed for 1 day at the temperature of 16 ℃, the number of dead insects in each well is counted by observing and counting under an orinbis inverted microscope. LC calculation by LD50 data processing software (C) blue universe studio Version 1.0150The results are shown in Table 1.
By combining all indexes, the 863 suspending agent 6 has the best effect, and has better fluidity and dispersibility and better storage resistance. In this example, the suspending agent before heat storage at 54 ℃ is a fresh suspension agent.
TABLE 1 indoor Activity assay results for C.elegans with Bacillus thuringiensis suspension for killing Meloidogyne incognita
TABLE 2 indices of each drug after 6 months storage at room temperature (20 ℃. + -. 5 ℃)
Example 4:
the field activity of the southern root-knot nematode killing bacillus thuringiensis suspending agent on the tomato root-knot nematodes is determined:
the test was carried out in 2017 in a greenhouse with severe tomato root-knot nematodes in soil in the beginning of Lichuan Jian county, Hubei province. The healthy tomato seedlings are transplanted to a greenhouse containing eggs of root-knot nematodes and then the treatments are started 1 week later.
Set 3 treatment groups: bt NBIN-863 biological control group, 21% fosthiazate avermectin chemical control group and clear water control group.
Biological group: the tomato strains are all alive and grow vigorously, root nodules are hardly seen, the tomatoes can be harvested normally, the disease condition is grade 1, the disease index is 17.13%, and the control effect is 81.46%. Chemical group: tomato plants survive, but have large and many nodules, the yield is reduced by 40%, the disease condition is grade 3, the disease index is 59.88, and the prevention effect is 35.18%.
Control group: the control group and more than ten greenhouse which are not treated are totally harvested, most plants die, the disease condition is 4 grade, and the disease index is 92.38 percent.
The disease index and control effect were calculated according to the following formula (Yang Ji et al, 2017):
disease index [ ∑ (number of plants per disease grade × number of representative plants on that grade)/(total number of plants × 7) ] × 100%
The prevention and treatment effect is [ (control disease index-treatment disease index)/control disease index ] × 100%
The effective period and the dosage of the medicament are 75 days, 1.5 kg/mu and 80 yuan/mu cost of the biological group effective period, 60 days, 2 kg/mu and 120 yuan/mu cost of the chemical group effective period.
The medicines are all newly prepared, and the formula for Bt NBIN-863 biological control group is 863 suspending agent 6.
Table 3 results of treatment groups on control of tomato root-knot nematode
Example 5:
the field activity of the southern root-knot nematode killing bacillus thuringiensis suspending agent on cucumber root-knot nematodes is determined:
the test was carried out in 2019 in a greenhouse with severe meloidogyne cucurbitae in soil of tiger river of Jingzhou city, Hubei province. The healthy cucumber seedlings are transplanted to a greenhouse containing the eggs of the root-knot nematodes and then are treated 1 week later.
Set 3 treatment groups: bt NBIN-863 biological control group, 20% fosthiazate chemical control group and clear water control group. The results of the cucumber after harvesting are checked, the biological group control effect reaches 70.01 percent, and the chemical group control effect reaches 61.31 percent.
The control effect was calculated in the same manner as in example 4.
The medicines are all newly prepared, and the formula for Bt NBIN-863 biological control group is 863 suspending agent 6.
Table 4 results of treatment groups on control of cucumber root-knot nematode
Example 6:
the field activity of the southern root-knot nematode killing bacillus thuringiensis suspending agent on the radish root-knot nematodes is determined:
the test was carried out in 2016 in a greenhouse with severe meloidogyne radish in Sanyo soil of Jingmen, Hubei. Radish seeds are sowed in a greenhouse containing eggs of root-knot nematodes, grown to the seedling stage for 2 weeks, and then subjected to various treatments.
Set 3 treatment groups: bt NBIN-863 biological control group, 1.8% avermectin missible oil chemical control group and clear water control group.
The medicines are all newly prepared, and the formula for Bt NBIN-863 biological control group is 863 suspending agent 6.
After the radish is harvested, the results are checked, the biological group control effect reaches 68.23 percent, the 1.8 percent avermectin emulsifiable solution chemical group control effect is 39.36 percent, the use amount of chemical pesticides is reduced by more than 50 percent, and the commodity of the radish is improved.
The control effect was calculated in the same manner as in example 4.
Table 5 root knot nematode control results of treatment groups
Example 7:
the field activity of the southern root-knot nematode killing bacillus thuringiensis suspending agent on lettuce root-knot nematodes is determined:
the test was carried out in a greenhouse with serious lettuce root knot nematodes in soil of tiger river of Jingzhou city, Hubei province in 2020. And transplanting healthy lettuce seedlings into a greenhouse containing eggs of root-knot nematodes, and starting each treatment 1 week later.
5 treatment groups were set: a dazomet control group, a Bt NBIN-863 microbial soil treatment agent biological control group, a dazomet + Bt NBIN-863 microbial soil treatment agent control group (firstly using dazomet to disinfect soil and then carrying out 863 root irrigation treatment), a fosthiazate chemical control group and a clear water control group.
The medicines are all newly prepared, and the formula for Bt NBIN-863 biological control group is 863 suspending agent 6.
The control effect of the dazomet control group is 55.35%, the control effect of the dazomet and microbial soil treatment agent control group is 64%, the control effect of the Bt NBIN-863 microbial soil treatment agent is 46.45%, and the control effect of the fosthiazate chemical group is 42.3%.
The control effect was calculated in the same manner as in example 4.
Table 6 results of treatment groups on lettuce root-knot nematode control
Claims (6)
1. The bacillus thuringiensis suspending agent for killing meloidogyne incognita comprises the following components in parts by weight: 93.5-96.2 of Bacillus thuringiensis fermentation stock solution, 0.3-0.5 of potassium sorbate, 0.5-1.0 of magnesium aluminum silicate, 3-5 of calcium lignosulfonate and 4.5-5.5 of pH value; the effective bacterium concentration of the bacillus thuringiensis in the suspending agent is 52-79 multiplied by 108CFU/mL; the preservation number of the bacillus thuringiensis in the suspending agent is CCTCC NO: m2013612.
2. The suspending agent according to claim 1, which comprises the following components in parts by weight: 93.75-95.85 of Bacillus thuringiensis fermentation stock solution, 0.4-0.5 of potassium sorbate, 0.5-1.0 of magnesium aluminum silicate, 3-5 of calcium lignosulphonate and 4.5-5.5 of pH value.
3. The suspending agent according to claim 1, which comprises the following components in parts by weight: 93.75-95.00 of Bacillus thuringiensis fermentation stock solution, 0.4-0.5 of potassium sorbate, 0.5-1.0 of magnesium aluminum silicate, 43-5 of calcium lignosulphonate and 4.5-5.5 of pH value.
4. Use of a suspension according to claim 1 for the preparation of a pesticide for controlling meloidogyne incognita or caenorhabditis elegans.
5. The use of claim 4, wherein said Meloidogyne incognita comprises: tomato, cucumber, lettuce or radish root knot nematodes.
6. Use of the suspension of claim 1 in the preparation of a field insecticide for the control of meloidogyne incognita.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110115748.6A CN112868672A (en) | 2021-01-28 | 2021-01-28 | Bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110115748.6A CN112868672A (en) | 2021-01-28 | 2021-01-28 | Bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112868672A true CN112868672A (en) | 2021-06-01 |
Family
ID=76052927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110115748.6A Pending CN112868672A (en) | 2021-01-28 | 2021-01-28 | Bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112868672A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114606155A (en) * | 2022-01-25 | 2022-06-10 | 云南大学 | Application of bacillus thuringiensis Bt79 and/or Bt80 strain in prevention and treatment of plant meloidogyne incognita |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6346262B1 (en) * | 1995-03-17 | 2002-02-12 | Lee County Mosquito Control District | Controlled delivery compositions and processes for treating organisms in a column of water or land |
| CN101481668A (en) * | 2009-01-15 | 2009-07-15 | 山东省科学院中日友好生物技术研究中心 | Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use |
| CN101884326A (en) * | 2010-07-15 | 2010-11-17 | 新沂中凯农用化工有限公司 | Preparation and application technology for waxy bacillus suspension agent |
| CN103898025A (en) * | 2014-04-04 | 2014-07-02 | 湖北省生物农药工程研究中心 | Bacillus thuringiensis for killing meloidogyn incognita and culture method of bacillus thuringiensis |
| CN105660706A (en) * | 2016-01-27 | 2016-06-15 | 山东省科学院生物研究所 | Application of bacillus cereus BCJB01 in control of root-knot nematodes |
-
2021
- 2021-01-28 CN CN202110115748.6A patent/CN112868672A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6346262B1 (en) * | 1995-03-17 | 2002-02-12 | Lee County Mosquito Control District | Controlled delivery compositions and processes for treating organisms in a column of water or land |
| CN101481668A (en) * | 2009-01-15 | 2009-07-15 | 山东省科学院中日友好生物技术研究中心 | Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use |
| CN101884326A (en) * | 2010-07-15 | 2010-11-17 | 新沂中凯农用化工有限公司 | Preparation and application technology for waxy bacillus suspension agent |
| CN103898025A (en) * | 2014-04-04 | 2014-07-02 | 湖北省生物农药工程研究中心 | Bacillus thuringiensis for killing meloidogyn incognita and culture method of bacillus thuringiensis |
| CN105660706A (en) * | 2016-01-27 | 2016-06-15 | 山东省科学院生物研究所 | Application of bacillus cereus BCJB01 in control of root-knot nematodes |
Non-Patent Citations (2)
| Title |
|---|
| 余洁等: "苏云金芽孢杆菌对新秀丽小杆线虫的作用", 《农业生物技术学报》 * |
| 沈晋良主编: "《农药加工与管理》", 30 June 2002, 中国农业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114606155A (en) * | 2022-01-25 | 2022-06-10 | 云南大学 | Application of bacillus thuringiensis Bt79 and/or Bt80 strain in prevention and treatment of plant meloidogyne incognita |
| CN114606155B (en) * | 2022-01-25 | 2023-08-04 | 云南大学 | Application of bacillus thuringiensis Bt79 and/or Bt80 strain in preventing and controlling plant southern root-knot nematode |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3046167B2 (en) | Plant disease control bacterium, control agent using the same, method for producing and use of control agent | |
| US10856551B2 (en) | Use of Bacillus methylotrophicus as a stimulant of plant growth and biological control means, and isolates of said species | |
| JP2000513582A (en) | Antimicrobial microbial preparation, its production method and treatment method | |
| CN101595892B (en) | PS mixture of compound biocontrol fungicide for preventing soil-borne diseases of vegetables | |
| WO2007011025A1 (en) | Agent for controlling diseases occurring in the stage of raising rice seedlings | |
| JP2009511012A (en) | Composition for controlling plant diseases comprising Bacillus subtilis KCCM10639 or KCCM10640 and method for controlling plant diseases using these | |
| US5403583A (en) | Bacillus megetarium ATCC 55000 and method of use thereof to control R. solani | |
| CN112868672A (en) | Bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof | |
| KR20190136009A (en) | Strains belonging to the genus Mitsaria and microbial pesticides using the strains | |
| CN113575614A (en) | Beauveria bassiana dispersible oil suspending agent and efficient use method | |
| CN109749940B (en) | Preparation method of fungal inoculant, product and application thereof | |
| WO2020262612A1 (en) | Plant disease control agent and plant disease control method | |
| CN112063543A (en) | Urease-producing bacterium and application thereof in preparation of microbial inoculum for preventing and treating tobacco root knot nematode disease | |
| CN107087640B (en) | Bactericidal composition containing bacillus amyloliquefaciens and prochloraz and application thereof | |
| KR101389975B1 (en) | culture medium for preventing disease and promoting growth of plant, manufacturing method of microbial agent using the same | |
| CN112646735B (en) | Metarhizium anisopliae, microbial insecticide, preparation method and application | |
| JPH06133763A (en) | Novel microorganism and blight control agent | |
| KR102075073B1 (en) | Serratia sp. KUDC3025 strain having antifungal activity against pathogens and plant growth promoting effect, and uses thereof | |
| KR101993986B1 (en) | New Paecilomyces lilacinus JEF076 and Purpureocillium lilacinum JEF141 strains controlling Orthoptera order, formulated compositions for controlling the Orthoptera order and application methods to control the Orthoptera order | |
| CN111758742A (en) | Microbial pesticide synergist suitable for wheat and application thereof | |
| CN116114712B (en) | Bactericidal composition containing bacillus amyloliquefaciens and application thereof | |
| RU2760337C1 (en) | Preparation for increasing the yield of spring wheat | |
| RU2800426C9 (en) | Bacillus amyloliquefaciens p20 strain as an agent for combating potato rhizoctoniosis | |
| CN115786172B (en) | Bacillus bailii and composition and application thereof | |
| KR20030087289A (en) | The antagonistic microorganism bacillus sp. big21003 and the preparation method of pelleted seed including thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210601 |
|
| WD01 | Invention patent application deemed withdrawn after publication |