CN111758742A - Microbial pesticide synergist suitable for wheat and application thereof - Google Patents
Microbial pesticide synergist suitable for wheat and application thereof Download PDFInfo
- Publication number
- CN111758742A CN111758742A CN202010703723.3A CN202010703723A CN111758742A CN 111758742 A CN111758742 A CN 111758742A CN 202010703723 A CN202010703723 A CN 202010703723A CN 111758742 A CN111758742 A CN 111758742A
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- Prior art keywords
- wheat
- microbial
- pesticide
- pesticide synergist
- microbial pesticide
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
Abstract
The invention discloses a microbial pesticide synergist suitable for wheat, which comprises the following components in percentage by weight: 18-25% of copper sulfate, 45-65% of zinc sulfate and the balance of dihydrocapsaicin. The pesticide synergist is applied to a pesticide containing Brevibacillus laterosporus, has a remarkable enhancement effect on the Brevibacillus laterosporus, and particularly has a control effect of more than 65% on Pseudomonas syringae (Pseudomonas syringae) causing wheat bacterial leaf blight. The microbial pesticide synergist comprises the main components of inorganic salt and natural plant extracts, does not contain organic pesticide components harmful to human bodies, is matched with microbial pesticide components for use, and is a safe and pollution-free pesticide.
Description
Technical Field
The invention relates to the technical field of fertilizers, in particular to a microbial pesticide synergist suitable for wheat and application thereof.
Background
Microbial fertilizers are also called biological fertilizers, inoculants or bacterial fertilizers (bacterial manures) and the like, and refer to a fertilizer product which takes the life activities of microorganisms as the core and enables crops to obtain specific fertilizer effects. The microbial fertilizer and the micro-fertilizer have essential differences: the former are living life and the latter are mineral elements. The microbial resources are rich, the variety and the functions are various, and the fertilizer can be developed into fertilizers with different functions and different purposes. And the microbial strains can be artificially bred, continuously purified and rejuvenated to improve the activity of the strains, and particularly, with the further development of biotechnology, the acquisition of the required strains by a genetic engineering method becomes possible.
Microbial fertilizers are of many types, and microbial fertilizer products are generally divided into two main categories: one is a microbial fertilizer in the narrow sense, which means that the supply of plant nutrient elements is increased through the life activities of microorganisms, and comprises the steps of increasing the total supply of the plant nutrient elements in soil and production environment, so that the nutrient condition of the plants is improved, and the yield is increased, wherein the representative variety of the microbial fertilizer is rhizobium fertilizer; the other is a broad-sense microbial fertilizer, which means that the supply of plant nutrient elements can be improved through the life activities of microorganisms in the microbial fertilizer, plant growth hormone can be generated, the absorption and utilization of the nutrient elements by plants are promoted or the pathogenic effect of certain pathogenic microorganisms is antagonized, the crop diseases and insect pests are reduced, and the crop yield is increased.
The invention patent with the application number of 201410623410.1, namely a saline-alkali tolerant microbial flora and application thereof, discloses a saline-alkali tolerant compound microbial active flora, discloses the existing common Shewanella, pseudomonas and halomonas, but the saline-alkali tolerant compound microbial active flora is mainly used for treating waste drilling mud and oily sludge with high saline-alkali content. The invention patent 'Huanghe Halomonas BJGMM-B45 and application thereof' with the application number of 201210572369.0 discloses a bacterium with excellent salt tolerance, namely, the Huanghe Halomonas BJGMM-B45, and the bacterium is disclosed to have antibiotic resistance and can be used as a gene donor of a new transgenic organism, but other special effects are not described in detail. The invention patent with application number 201310466328.8, namely a method for improving saline-alkali soil by using microorganisms, discloses a method for improving saline-alkali soil by using a mixed microbial inoculum of halomonas elongata, bacillus lignicolis and bacillus alkalescens, but the three strains contained in the technical scheme are the conventional common strains, only the microbial preparation strain powder is disclosed to improve the germination rate of corn and tomato, and more beneficial effects are not recorded.
The invention patent CN107151641A discloses a Brevibacillus laterosporus for inhibiting rice sheath blight and application thereof, the Brevibacillus laterosporus SNB10 for inhibiting rice sheath blight is adopted, the control effect on rice sheath blight is good, the strain can produce chitinase, the bacteriostasis spectrum is wide, and the strain has a certain inhibiting effect on various plant pathogenic fungi and plant pathogenic bacteria. But the pesticide effect of the pesticide prepared by the strain still has a larger improvement space. Therefore, the invention provides a microbial pesticide synergist suitable for wheat.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a microbial pesticide synergist suitable for wheat.
The technical scheme of the invention is as follows:
a microbial pesticide synergist suitable for wheat comprises the following components: copper sulfate, zinc sulfate and dihydrocapsaicin.
Preferably, the microbial pesticide synergist suitable for wheat comprises the following components in percentage by weight: 18-25% of copper sulfate, 45-65% of zinc sulfate and the balance of dihydrocapsaicin.
Preferably, the dihydrocapsaicin is extracted from natural plant capsicum.
Preferably, the microbial pesticide synergist is applied to a pesticide containing brevibacillus laterosporus.
Further preferably, the brevibacillus laterosporus is brevibacillus laterosporus SNB10, and the strain is deposited in China general microbiological culture Collection center on 5-17 th month in 2017 with the deposition numbers: 14171.
further preferably, the microbial pesticide synergist suitable for wheat can be used for controlling pseudomonas syringae (pseudomonas syringae).
The invention has the advantages that: the microbial pesticide synergist suitable for wheat of the invention comprises the following components in percentage by weight: 18-25% of copper sulfate, 45-65% of zinc sulfate and the balance of dihydrocapsaicin. The pesticide synergist is applied to a pesticide containing Brevibacillus laterosporus, has a remarkable enhancement effect on the Brevibacillus laterosporus, and particularly has a control effect of more than 65% on Pseudomonas syringae (Pseudomonas syringae) causing wheat bacterial leaf blight. The microbial pesticide synergist comprises the main components of inorganic salt and natural plant extracts, does not contain organic pesticide components harmful to human bodies, is matched with microbial pesticide components for use, and is a safe and pollution-free pesticide.
Detailed Description
Example 1
A microbial pesticide synergist suitable for wheat comprises the following components in percentage by weight: 22% of copper sulfate, 60% of zinc sulfate and the balance of dihydrocapsaicin.
The dihydrocapsaicin is extracted from natural plant Capsici fructus.
Example 2
A microbial pesticide synergist suitable for wheat comprises the following components in percentage by weight: 25% of copper sulfate, 45% of zinc sulfate and the balance of dihydrocapsaicin.
The dihydrocapsaicin is extracted from natural plant Capsici fructus.
Example 3
A microbial pesticide synergist suitable for wheat comprises the following components in percentage by weight: 18 percent of copper sulfate, 65 percent of zinc sulfate and the balance of dihydrocapsaicin.
The dihydrocapsaicin is extracted from natural plant Capsici fructus.
Example 4
A microbial pesticide synergist suitable for wheat comprises the following components in percentage by weight: 20% of copper sulfate, 65% of zinc sulfate and the balance of dihydrocapsaicin.
The dihydrocapsaicin is extracted from natural plant Capsici fructus.
Example 5
A microbial pesticide synergist suitable for wheat comprises the following components in percentage by weight: 20% of copper sulfate, 55% of zinc sulfate and the balance of dihydrocapsaicin.
The dihydrocapsaicin is extracted from natural plant Capsici fructus.
The pesticide synergist of the above examples 1-5 is mixed with the Brevibacillus laterosporus SNB10 microbial inoculum, wherein the number of live spores of the Brevibacillus laterosporus microbial inoculum is 1-2 × 109cfu/mL。
Comparative example 1
The dihydrocapsaicin in example 1 was removed and the remaining ratio was unchanged.
Comparative example 2
The copper sulfate and the zinc sulfate in example 1 were removed simultaneously, and the remaining mixture ratio was unchanged.
1. Test material
Pesticide synergist 1g and viable spore number of 1-2 × 10 in examples 1-5910mL of cfu/mL Brevibacillus laterosporus SNB10 microbial inoculum, 1L of water is added to obtain the pesticide, the contrast agent is antiviral pesticide ningnanmycin, and clear water spraying is a blank contrast.
Pathogen: pseudomonas syringae (Pseudomonas syringae)
Host plant: wheat (Triticum aestivum L.)
2. Test method
The area of the test area was 26.6 square meters, 300 holes per cell, four replicates. Spraying the first time at the seedling stage (sporadic incidence) of wheat for 2 times at intervals of 5-7 days, wherein the spraying amount of the pesticide is 40L/mu.
3. Investigation method
Investigating all plants in each cell, investigating the disease grading standard according to the industry standard of preventing and treating wheat virus diseases, and dividing into 7 grades:
level 0: the whole plant is disease-free;
level 1: the plant is not obviously dwarfed and is shorter than a healthy plant by less than 20 percent;
and 3, level: dwarfing the plant, wherein the height of the plant is 20-35% shorter than that of a healthy plant;
and 5, stage: the plant is seriously dwarfed, and the height of the plant is 35 to 50 percent shorter than that of a healthy plant;
and 7, stage: the plant is severely dwarfed, and the height of the plant is shorter than that of a healthy plant by more than 50 percent or the plant dies.
4. Test results
The disease index and control effect of each treatment are as follows:
5. and (4) conclusion:
the pesticide synergist can obviously enhance the control effect of the brevibacillus laterosporus on the pseudomonas syringae.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The microbial pesticide synergist for wheat is characterized by comprising the following components: copper sulfate, zinc sulfate and dihydrocapsaicin.
2. The microbial pesticide synergist applicable to wheat as claimed in claim 1, which consists of the following components in percentage by weight: 18-25% of copper sulfate, 45-65% of zinc sulfate and the balance of dihydrocapsaicin.
3. A microbial pesticide synergist for use on wheat as claimed in claim 1 or claim 2 wherein said dihydrocapsaicin is extracted from the natural plant capsicum.
4. The microbial enhancer for agricultural chemicals suitable for wheat according to claim 1 or 2, wherein the microbial enhancer for agricultural chemicals is used in agricultural chemicals containing Brevibacillus laterosporus.
5. The microbial pesticide synergist for wheat as claimed in claim 4, wherein said Brevibacillus laterosporus is Brevibacillus laterosporus SNB10, which has been deposited in China general microbiological culture Collection center on 5.17.2017 with the following deposition numbers: 14171.
6. the microbial pesticide synergist for wheat as claimed in any one of claims 1-5, wherein the microbial pesticide synergist for wheat is used for controlling pseudomonas syringae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010703723.3A CN111758742A (en) | 2020-07-18 | 2020-07-18 | Microbial pesticide synergist suitable for wheat and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010703723.3A CN111758742A (en) | 2020-07-18 | 2020-07-18 | Microbial pesticide synergist suitable for wheat and application thereof |
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| Publication Number | Publication Date |
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| CN111758742A true CN111758742A (en) | 2020-10-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN202010703723.3A Withdrawn CN111758742A (en) | 2020-07-18 | 2020-07-18 | Microbial pesticide synergist suitable for wheat and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113383795A (en) * | 2021-07-05 | 2021-09-14 | 德州学院 | Wheat microbial pesticide synergist and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102976840A (en) * | 2011-09-05 | 2013-03-20 | 麻林涛 | Specialized composite microbial fertilizer for corn, and preparation method thereof |
| CN107151641A (en) * | 2017-07-04 | 2017-09-12 | 沈阳农业大学 | One plant suppression Rhizoctonia solani Kuhn Brevibacillus laterosporus and its application |
| US20170290332A1 (en) * | 2010-04-09 | 2017-10-12 | Pacific Tech Industries, Inc. | Grease-like gel for repelling rodents |
| CN110771630A (en) * | 2019-10-15 | 2020-02-11 | 汤怀信 | Insecticide |
| CN111394361A (en) * | 2018-12-27 | 2020-07-10 | 中粮生物科技（北京）有限公司 | Brevibacillus laterosporus, composition and application thereof |
-
2020
- 2020-07-18 CN CN202010703723.3A patent/CN111758742A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170290332A1 (en) * | 2010-04-09 | 2017-10-12 | Pacific Tech Industries, Inc. | Grease-like gel for repelling rodents |
| CN102976840A (en) * | 2011-09-05 | 2013-03-20 | 麻林涛 | Specialized composite microbial fertilizer for corn, and preparation method thereof |
| CN107151641A (en) * | 2017-07-04 | 2017-09-12 | 沈阳农业大学 | One plant suppression Rhizoctonia solani Kuhn Brevibacillus laterosporus and its application |
| CN111394361A (en) * | 2018-12-27 | 2020-07-10 | 中粮生物科技（北京）有限公司 | Brevibacillus laterosporus, composition and application thereof |
| CN110771630A (en) * | 2019-10-15 | 2020-02-11 | 汤怀信 | Insecticide |
Non-Patent Citations (4)
| Title |
|---|
| GEUN CHEOL SONG ET AL.: "Elicitation of Induced Resistance against Pectobacterium carotovorum and Pseudomonas syringae by Specific Individual Compounds Derived from Native Korean Plant Species", 《MOLECULES》 * |
| 林晓安 等主编: "《河南林业有害生物防治技术》", 30 September 2005, 黄河水利出版社 * |
| 陈三斌 主编: "《农用化工产品手册》", 30 April 2008, 金盾出版社 * |
| 陈士林 主编: "《中华医学百科全书 中药资源学》", 30 April 2018, 中国协和医科大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113383795A (en) * | 2021-07-05 | 2021-09-14 | 德州学院 | Wheat microbial pesticide synergist and preparation method thereof |
| WO2023279418A1 (en) * | 2021-07-05 | 2023-01-12 | 德州学院 | Wheat microbial pesticide synergist, and preparation method therefor |
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