CN101884326A - Preparation and application technology for waxy bacillus suspension agent - Google Patents
Preparation and application technology for waxy bacillus suspension agent Download PDFInfo
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- CN101884326A CN101884326A CN2010102268327A CN201010226832A CN101884326A CN 101884326 A CN101884326 A CN 101884326A CN 2010102268327 A CN2010102268327 A CN 2010102268327A CN 201010226832 A CN201010226832 A CN 201010226832A CN 101884326 A CN101884326 A CN 101884326A
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- waxy bacillus
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Abstract
The invention relates to preparation and application technology for a waxy bacillus suspension agent. The suspension agent is characterized by comprising the following components of: usually 1 to 1,010 bacterial colonies of living body waxy bacillus per milliliter, 1 to 25 weight percent of dispersing agent, 0.1 to 5 weight percent of thickening agent and the balance of water. The waxy bacillus suspension agent can be used for efficiently preventing and controlling root-knot nematodes of greenhouse vegetables such as watermelons, balsam pears, west melons, cucumbers, tomatoes, towel gourds, celeries and the like and outdoor vegetables. The using method comprises the following steps of: (1) after applying farmyard manure on a soil surface, diluting 1,000 milliliters of the waxy bacillus suspension agent by using 40 to 50 kilograms of water, uniformly spraying the diluted suspension agent on the soil surface, and turning soil; and transplanting crops after 3 to 10 days, wherein the effect of preventing and controlling the root-knot nematodes of the vegetables can reach 70 to 90 percent; and (2) after conventional fertilization of the soil, diluting 6,000 milliliters of the waxy bacillus suspension agent by using water until the diluted volume is 40 to 50 times the original volume of the suspension agent and irrigating roots by using the diluted suspension agent which serves as root-fixing water when transplanting the crops. The waxy bacillus suspension agent can efficiently prevent and control the root-knot nematodes of the vegetables.
Description
Technical field:
The present invention is a kind of preparation and application technology of waxy bacillus suspension agent.
Background technology:
Root-knot nematode is a root system of plant obligate endoparasite, and annual agricultural production to China has caused very big loss.Investigation by throughout the country shows; booth vegetable such as watermelon, balsam pear, western muskmelon, cucumber, tomato, sponge gourd, celery and outdoor vegetable plot almost all have nematodiasis in various degree to take place; especially even more serious at protection ground continuous cropping cultivation average attack rate, weight person's total crop failure.As a kind of soil-borne disease, nematodosis is prevented and treated very difficulty.At present mainly prevent and treat nematodiasis with chemical insecticides such as thiophene worm phosphorus, carbosulfan, cadusafos, Celfumes.Even, use furans pellet, the contour insecticide that poisons of Aldicarb to be used to prevent and treat root-knot nematode in some serious zones of root-knot nematode harm.A large amount of uses of chemical insecticide cause crop residue of pesticide severe overweight on the one hand, and personnel's poisoning takes place often, owing to use high-toxic pesticide continuously, have destroyed soil structure on the one hand, cause soil to desertify, and the pesticide resistance problem are very outstanding again.
Waxy Bacillus (Bacillus cereus) is a kind of microorganism that obtains that separates in soil, and by discovering, it can prevent and treat the root-knot nematode of vegetables.Significant feature mechanism is mainly to comprise: (1) plant rhizosphere decide grow, preferentially capture and grow the site surely, reduce the absorption of pathogen; (2) inducing plant disease resistance; (3) promote plant growing by multiple modes such as phosphorus decomposing, fixed nitrogen, generation heteroauxins, make resistivity and the increasing both production and income of its enhancing disease; (4) produce one or more antibiotic or hydrolase, pathogen is had killing or the stunning effect to a certain degree.Root knot nematode disease is the disease that has a strong impact on China's plant production development, and the control of falling ill for soil-borne diseases such as root-knot nematodes is always based on chemical agent.But chemical nematocide environmental pollution is serious, and is also dangerous to people, animal in the use, so the use of biopesticide is at home and abroad all paid much attention to.
By to the use of waxy Bacillus research, find that it can effectively prevent and treat root knot nematode under certain using dosage, control efficiency can reach 50%-70%, has solved the problem of preventing and treating of root-knot nematode well.
The preparation of the waxy Bacillus that uses on market at present is mainly the wetting powder of deactivation etc., and the use of live body suspending agent yet there are no report.
Summary of the invention:
Active ingredient is among the present invention: waxy Bacillus
The clump count that contains the live body waxy Bacillus in every milliliter of the suspending agent of the present invention is 1-10
10Individual.
Adoptable surface-active has anionic surfactant and nonionic surface active agent.The aliphatic alcohol sulfate that comprises Sulfates, alkylol polyoxyethylene groups ether sodium sulfate etc., the sodium alkyl sulfonate of Sulfonates, alkylbenzenesulfonate, alkylnaphthalene sulfonate, dialkyl succinylsuccinate sulfonate, monoalkyl phenyl polyoxyethylene groups ether succinic acid sulfonate, fatty alcohol ethylene oxide adduct sulfonate, alkylphenol formaldehyde condensation product ethylene oxide adduct sulfonate, fatty alcohol epoxy vinyl ether, alkylphenol-polyethenoxy base ether, the anhydrous sorbitol fatty acid ester, anhydrous sorbitol aliphatic acid polyethenoxy base ether, alkylphenol formaldehyde condensation product polyoxyethylene groups ether, sulfonation castor-oil plant alkoxide, quaternary ammonium salt derivative, the condensation product of oxirane and nonyl or octyl phenol is main product or base and the oxirane that does not dissociate by esterification, the alkalies and alkaline earth salt of sulfuric ester and sulfonic acid is as sulphonyl amber ester octyl group sodium in two ninth of the ten Heavenly Stems and such as the alkali of the macromolecule sulfonic acid derivative of sodium lignin sulfonate and calcium and carboxylate that the alkali salt condensation forms the anhydrous sorbierite of looking into dissolubility etc.That can give an example has farming breast No. 2000 series, petroleum sodium sulfonate, dodecyl sodium sulfates, draws back powder, ethoxylated dodecyl alcohol sodium sulfonate, tween series etc.
Adoptable thickener has xanthans, organobentonite, Magnesiumaluminumsilicate, white carbon, diatomite, carboxymethyl cellulose etc.
Suspending agent of the present invention is active ingredient waxy Bacillus, wetting dispersing agent, mixtures of thickening agents.Usually the clump count that contains the live body waxy Bacillus in the suspending agent of this invention is a 1-1010/milliliter, the dispersant of 1-25% (weight), and the thickener of 0.1-5%, surplus makes water supply 100%.
Suspending agent processing technology of the present invention is: waxy Bacillus, the wetting agent of metering, dispersant, the thickener with metering mixes fully earlier, and wet grinding can obtain suspending agent.
Suspending agent provided by the present invention can be used for preventing and treating the root-knot nematode of booth vegetables such as watermelon, balsam pear, western muskmelon, cucumber, tomato, sponge gourd, celery and outdoor vegetable.
The using method of suspending agent of the present invention is:
1, farmyard manure is imposed on soil surface after, 1000 milliliters waxy bacillus suspension agents are diluted in the 40-50 kg of water, with sprayer evenly spray sweep to soil surface, plough.Transplant crop after 3-10 days, can reach 70-90% the control efficiency of root knot nematode.
2, behind the soil conventional fertilizer application, in vegetable transplanting, every mu is used 6000 milliliters of waxy bacillus suspension agent dilution 40-50 doubly, irritates root as normal root water when transplanting, and can prevent and treat root knot nematode effectively.
Embodiment:
Following examples describe the present invention in detail, and embodiment mainly is for the present invention is described but not is used for limiting the present invention.
Embodiment 1: the formulation preparation example
The Magnesiumaluminumsilicate that adds metering in the water of metering fully after the stirring and dissolving, counts the waxy Bacillus that an amount of nonionic emulsifier adds metering simultaneously and can form suspending agent under stirring fully fast.
Embodiment 2: using method embodiment
(1) control efficiency of 1 pair of root-knot nematode of employing using method.
The place: period is investigated in the Xinyi: the 90 day time behind the medicine: 2009
| Medicament | Using dosage (milliliter/mu) | Preventive effect (%) |
| Waxy bacillus suspension agent | ??1000 | ??72.5 |
| Waxy bacillus suspension agent | ??1500 | ??84.3 |
| Waxy bacillus suspension agent | ??2000 | ??90.6 |
(2) control efficiency of 2 pairs of root-knot nematodes of employing using method.
The place: period is investigated in the Xinyi: the 90 day time behind the medicine: 2009
| Medicament | Using dosage (milliliter/mu) | Preventive effect (%) |
| Waxy bacillus suspension agent | ??5000 | ??69.8 |
| Waxy bacillus suspension agent | ??6000 | ??81.2 |
| Waxy bacillus suspension agent | ??7000 | ??89.7 |
Claims (3)
1. the preparation of a waxy bacillus suspension agent and application technology is characterized in that comprising processing method and operation technique etc.
2. according to the preparation of the described waxy bacillus suspension agent of claim 1 and application technology preparation and application technology, the clump count that it is characterized in that containing usually in the suspending agent of this invention the live body waxy Bacillus is 1-10
10Individual/milliliter, the dispersant of 1-25% (weight), the thickener of 0.1-5%, surplus makes water supply 100%.
3. according to claim 1, the using method of waxy bacillus suspension agent is: (1), farmyard manure imposed on soil surface after, 1000 milliliters waxy bacillus suspension agents are diluted in the 40-50 kg of water, evenly spray with sprayer and sweep to soil surface, plough.Transplant crop after 3-10 days, can reach 70-90% the control efficiency of root knot nematode.(2), behind the soil conventional fertilizer application, in vegetable transplanting, every mu is used 6000 milliliters of waxy bacillus suspension agents dilution 40-50 doubly, irritates root as normal root water when transplanting, and can prevent and treat root knot nematode effectively.
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| CN2010102268327A CN101884326A (en) | 2010-07-15 | 2010-07-15 | Preparation and application technology for waxy bacillus suspension agent |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154159A (en) * | 2010-12-29 | 2011-08-17 | 南京农业大学 | Antagonistic bacteria for preventing and controlling tomato root-knot nematodes and microorganism organic fertilizer produced by same |
| CN102321554A (en) * | 2011-08-30 | 2012-01-18 | 新疆农业科学院微生物应用研究所 | Bacillus cereus and its application of being as plant growth-promoting rhizobacteria |
| CN102578152A (en) * | 2012-01-16 | 2012-07-18 | 南京农业大学 | Bacillus cereus AR156 wettable powders as well as preparation and application thereof |
| CN105265245A (en) * | 2015-09-28 | 2016-01-27 | 常德泰谷生物肥料有限公司 | Method of controlling jute root knot nematode disease |
| CN105296366A (en) * | 2015-11-27 | 2016-02-03 | 青岛农业大学 | Compound microbial agent capable of promoting tomato growth and development and application thereof |
| CN105660706A (en) * | 2016-01-27 | 2016-06-15 | 山东省科学院生物研究所 | Application of bacillus cereus BCJB01 in control of root-knot nematodes |
| US9468215B2 (en) | 2013-01-30 | 2016-10-18 | Bio Insumos Nativa Spa | Bionematicide composition and method for controlling phytopathogenic nematodes using the same |
| CN108077313A (en) * | 2016-11-22 | 2018-05-29 | 上海农乐生物制品股份有限公司 | A kind of shenqinmycin waxy Bacillus compounding suspending agent and its preparation and application |
| CN109042742A (en) * | 2018-08-18 | 2018-12-21 | 杭州渔森农业技术开发有限公司 | Compound formulation is used to prevent and treat soil and passes the purposes fallen ill |
| CN111713206A (en) * | 2020-06-19 | 2020-09-29 | 四川省农业科学院植物保护研究所 | Method for increasing yield of bitter gourds by using biocontrol strain bacillus amyloliquefaciens Bam22 |
| CN112868672A (en) * | 2021-01-28 | 2021-06-01 | 湖北省生物农药工程研究中心 | Bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101028009A (en) * | 2007-04-03 | 2007-09-05 | 盐城苏滨生物农化有限公司 | Caprotrahol and Bacillus subtilis compound agricultural chemicals |
| CN101481668A (en) * | 2009-01-15 | 2009-07-15 | 山东省科学院中日友好生物技术研究中心 | Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use |
| CN101671641A (en) * | 2009-08-12 | 2010-03-17 | 南京农业大学 | Bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease |
-
2010
- 2010-07-15 CN CN2010102268327A patent/CN101884326A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101028009A (en) * | 2007-04-03 | 2007-09-05 | 盐城苏滨生物农化有限公司 | Caprotrahol and Bacillus subtilis compound agricultural chemicals |
| CN101481668A (en) * | 2009-01-15 | 2009-07-15 | 山东省科学院中日友好生物技术研究中心 | Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use |
| CN101671641A (en) * | 2009-08-12 | 2010-03-17 | 南京农业大学 | Bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease |
Non-Patent Citations (1)
| Title |
|---|
| 罗文辉等: "10%井·蜡芽菌悬浮剂防治水稻纹枯病试验初报", 《中国稻米》, no. 4, 20 July 2009 (2009-07-20), pages 67 - 68 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154159A (en) * | 2010-12-29 | 2011-08-17 | 南京农业大学 | Antagonistic bacteria for preventing and controlling tomato root-knot nematodes and microorganism organic fertilizer produced by same |
| CN102321554A (en) * | 2011-08-30 | 2012-01-18 | 新疆农业科学院微生物应用研究所 | Bacillus cereus and its application of being as plant growth-promoting rhizobacteria |
| CN102321554B (en) * | 2011-08-30 | 2012-12-05 | 新疆农业科学院微生物应用研究所 | Bacillus cereus and its application of being as plant growth-promoting rhizobacteria |
| CN102578152A (en) * | 2012-01-16 | 2012-07-18 | 南京农业大学 | Bacillus cereus AR156 wettable powders as well as preparation and application thereof |
| US9468215B2 (en) | 2013-01-30 | 2016-10-18 | Bio Insumos Nativa Spa | Bionematicide composition and method for controlling phytopathogenic nematodes using the same |
| CN105265245B (en) * | 2015-09-28 | 2018-01-19 | 常德泰谷生物肥料有限公司 | A kind of method for preventing and treating jute root knot nematode disease |
| CN105265245A (en) * | 2015-09-28 | 2016-01-27 | 常德泰谷生物肥料有限公司 | Method of controlling jute root knot nematode disease |
| CN105296366A (en) * | 2015-11-27 | 2016-02-03 | 青岛农业大学 | Compound microbial agent capable of promoting tomato growth and development and application thereof |
| CN105660706A (en) * | 2016-01-27 | 2016-06-15 | 山东省科学院生物研究所 | Application of bacillus cereus BCJB01 in control of root-knot nematodes |
| CN105660706B (en) * | 2016-01-27 | 2018-05-25 | 山东省科学院生物研究所 | Applications of the bacillus cereus BCJB01 in terms of root-knot nematode is prevented |
| CN108077313A (en) * | 2016-11-22 | 2018-05-29 | 上海农乐生物制品股份有限公司 | A kind of shenqinmycin waxy Bacillus compounding suspending agent and its preparation and application |
| CN109042742A (en) * | 2018-08-18 | 2018-12-21 | 杭州渔森农业技术开发有限公司 | Compound formulation is used to prevent and treat soil and passes the purposes fallen ill |
| CN111713206A (en) * | 2020-06-19 | 2020-09-29 | 四川省农业科学院植物保护研究所 | Method for increasing yield of bitter gourds by using biocontrol strain bacillus amyloliquefaciens Bam22 |
| CN112868672A (en) * | 2021-01-28 | 2021-06-01 | 湖北省生物农药工程研究中心 | Bacillus thuringiensis suspending agent for killing meloidogyne incognita and application thereof |
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Application publication date: 20101117 |