NL1039387C2

NL1039387C2 - Method for controlling plant disease in ornamental bulbous plants.

Info

Publication number: NL1039387C2
Application number: NL1039387A
Authority: NL
Inventors: Norifumi Yasuda; Atsushi Fukuchi
Original assignee: Sumitomo Chemical Co
Priority date: 2011-03-01
Filing date: 2012-02-17
Publication date: 2012-09-26
Also published as: NL1039387A; JP2012193163A

Description

METHOD FOR CONTROLLING PLANT DISEASE IN ORNAMENTAL BULBOUS PLANTS

Technical Field 5 The present invention relates to a method for controlling plant disease in ornamental bulbous plants.

Background Art A large number of chemical compounds for controlling 10 plant diseases have heretofore been developed and provided for practical use.

Tolclofos-methyl and azoxystrobin are known as compounds with plant disease controlling activity (see, The Pesticide Manual Thirteenth Edition (published by British Crop 15 Protection Council)).

However, these compounds are not satisfactory in some cases, and excellent methods have been needed for controlling plant diseases in accordance with the situation.

20 Disclosure of Invention

Thus, the present invention is claimed as follows: [1] A method for controlling plant diseases in an ornamental bulbous plant, comprising the steps of: treating, with an effective amount of tolclofos-methyl and 25 azoxystrobin, furrows with the depth of 1 to 40 cm and the width of 1 to 50 cm in the soil of a cultivation area for the ornamental bulbous plant; and planting bulbs of the ornamental bulbous plant in the furrows .

30 [2] The method of [1] , wherein the total amount of tolclofos-methyl and azoxystrobin is from 1 to 1000 kg per 10000 m2 of a cultivation area for an ornamental bulbous plant.

[3] The method of [1] or [2], wherein the amount of azoxystrobin is from 1 to 1000 parts by weight relative to 100 35 parts by weight of tolclofos-methyl.

1039387 2 [4] The method of any one of [1] to [3], wherein the ornamental bulbous plant is selected from the group consisting of tulip, lily, gladiolus and hyacinth.

[5] The method of any one of [1] to [3], wherein the 5 ornamental bulbous plant is tulip.

[6] A method for cultivating an ornamental bulbous plant, comprising the steps of: treating, with tolclofos-methyl and azoxystrobin in an amount effective for controlling plant diseases, furrows with 10 the depth of 1 to 40 cm and the width of 1 to 50 cm in the soil of a cultivation area for the ornamental bulbous plant; planting bulbs of the ornamental bulbous plant; and then filling, with soil, the furrows where bulbs has been planted.

15 [7] The method of [6], wherein the total amount of tolclofos-methyl and azoxystrobin is from 1 to 1000 kg per 10000 m2 of a cultivation area for an ornamental bulbous plant.

[8] The method of [6] or [7], wherein the amount of azoxystrobin is from 1 to 1000 parts by weight relative to 100 20 parts by weight of tolclofos-methyl.

[9] The method of any one of [6] to [8], wherein the ornamental bulbous plant is a plant selected from the group consisting of tulip, lily, gladiolus and hyacinth.

[10] The method of any one of [6] to [8], wherein the 25 ornamental bulbous plant is tulip

The present invention can control plant diseases in ornamental bulbous plants and is useful for growing ornamental bulbous plants.

30

Embodiment of the Invention

The method according to the present invention for controlling plant diseases comprises the steps of: treating, with an effective amount of tolclofos-methyl and 35 azoxystrobin, furrows with the depth of 1 to 40 cm and the width 3 of 1 to 50 cm in the soil of a cultivation area for the ornamental bulbous plant; and planting bulbs of the ornamental bulbous plant in the treated furrows.

5 Tolclofos-methyl and azoxystrobin are described, for example, on pages 979 and 52 of The Pesticide Manual, Thirteenth Edition (published by British Crop Protection Council).

These compounds may be commercially available formulations or produced using a known process. Alternatively, a 10 commercially available formulation containing tolclofos-methyl and/or azoxystrobin may be used in the present invention.

The amount of tolclofos-methyl to be used in the present invention is generally from 1 to 1000 parts and preferably from 10 to 500 parts by weight relative to 100 parts 15 by weight of tolclofos-methyl.

For the present invention, tolclofos-methyl and azoxystrobin may be simply admixed or combined. However, in general, as tolclofos-methyl and azoxystrobin, a formulation which contains an azoxystrobin and a formulation which contains 20 tolclofos-methyl are admixed or combined, or a formulation which contains tolclofos-methyl and azoxystrobin are used.

Such formulations are formulated into oil solutions, emulsions, flowables, water-dispersible powders, water-dispersible granules, dust powders, granules and the like which 25 can be obtained by mixing tolclofos-methyl and/or azoxystrobin with an inert carrier and, if necessary, adding a surfactant or other formulation additives. Such formulations contain an active compound(s), i.e., tolclofos-methyl and/or azoxystrobin in the total amount of usually from 0.1 to 99% and preferably 30 from 0.2 to 90% by weight of the formulations.

Solid carriers used for the formulation include fine powders or granules composed of minerals such as kaolin clay, attapulgite clay, bentonite, montmorillonite, acid white clay, pyrophyllite, talc, diatomaceous earth and calcite; natural 35 organic materials such as corn cob powder and walnut husk powder; synthetic organic materials such as urea; salts such as 4 calcium carbonate and ammonium sulfate; or synthetic inorganic materials such as synthetic hydrated silicon oxide. Liquid carriers include aromatic hydrocarbons such as xylene, alkylbenzene and methylnaphthalene; alcohols such as 2-propanol, 5 ethylene glycol, propylene glycol and ethylene glycol monoethyl ether; ketones such as acetone, cyclohexanone and isophorone; vegetable oil such as soybean oil and cotton seed oil; petroleum aliphatic hydrocarbons; esters; dimethylsulfoxide; acetonitrile; and water.

10 Surfactants include anionic surfactants, such as alkyl sulfate ester salts, alkyl aryl sulfonate salts, dialkyl sulfosuccinate salts, polyoxyethylene alkyl aryl ether phosphate ester salts, lignosulfonate salts and naphthalene sulfonate formaldehyde polycondensates; and non-ionic surfactants such as 15 polyoxyethylene alkyl aryl ethers, polyoxyethylene-alkylpolyoxypropylene block copolymers and sorbitan fatty acid esters; and cationic surfactants such as alkyltrimethylammonium salts .

Other formulation additives include water-soluble 20 polymers, such as polyvinyl alcohol and polyvinylpyrrolidone; polysaccharides such as Arabic gum, alginic acid and salts thereof, CMC (carboxymethyl-cellulose) and Xanthan gum; inorganic materials such as aluminum magnesium silicate and alumina sol; preservatives; coloring agents; and stabilizing 25 agents such as PAP (acid phosphate isopropyl) and BHT.

Ornamental bulbous plants to be used in the method of the present invention for controlling plant diseases include Lily (Lilium spp), Tulip (Tulipa gesneriana), Hyacinth (Hyacinthus orientalis), Muscari (Muscari latifolium), Allium 30 (Allium cristophii), Tritoma (Kniphofia spp), colchicum (Colchicum autumnale), Pineapple lily (Eucomis autumnalis), Sandasonia (Sandersonia aurantiaca), Gloriosa (Gloriosa superba), Narcissus (Narcissus spp), Daffodil (Narcissus pseudonarcissus), Amaryllis (Hippeastrum spp), snowdrop 35 (Galanthus spp), snowflake (Leucojum aestivum) , Diamond lily (Bodenii Nerine), Zefiransasu (Zephyranthes carinata), licorice 5 (Lycoris spp), Amazon lily (Eucharis grandiflora), Crocus (Crocus spp), Gladiolus (Gladiolus spp), Montbretia (Crocosmia χ crocosmiiflora), German iris (Iris spp), Saffron (Saffron crocus), Dahlias (dahlias spp), Ranunculus (Ranunculus 5 asiaticus), Curcuma (Curcuma spp), Oxalis (Oxalis deppei), Canna (Canna indica), and Cyclamen (Cyclamen persicum).

Among them, a plant selected from the group consisting of tulip, lily, gladiolus and hyacinth is preferred for the present invention.

10 Plant diseases that can be controlled by the present invention include soil-borne diseases, such as Bulb rot (Fusarium spp. Fusarium oxysporum, Fusarium solani, Rhizopus spp. Rhizopus necans, Rhizopus stolonifer), Damping off (Rhizoctonia spp. Rhizoctonia solani), Bare batch, brown batch, 15 Leaf rot (Rhizoctonia spp. Rhizoctonia solani), Bulb-coat rot (Rhizoctonia spp. Rhizoctonia solani), Sclerotial blight (Sclerotium spp. Sclerotium rolfaii), and Crown rot (Pythium spp. Pythium irregulare, P.spinosum, P. ultimum).

20 In the method of the present invention, the soil of a cultivation area for ornamental bulbous plants is provided with furrows which have been made by using a conventional furrow opener.

The size of the furrows is appropriately determined 25 depending on factors such as the varieties of the ornamental bulbous plants to be grown and the size of the bulbs. The furrows has the following size; the depth is from 1 to 40 cm, preferably from 5 to 15 cm, and the width is from 1 to 50 cm, preferably from 5 to 30 cm.

30

The method for treating such furrows with tolclofos-methyl and azoxystrobin may be implemented by spraying or injecting tolclofos-methyl and azoxystrobin, either in combination or separately, into the furrows prior to planting ornamental 35 bulbous plants. In this embodiment, tolclofos-methyl and azoxystrobin may be independently or together in the formulated 6 form of an oil solution, water-dispersible powders, water-dispersible granules, dust powders, granules, an emulsion or a flowable, or a water dilution thereof. After the treatment, bulbs are placed in the furrows and then covered with soil. By 5 conducting these steps, plant diseases caused by soil-borne pathogens that may occur during growth of the plants is controlled so that efficient cultivation of ornamental bulbous plants is possible.

In the present invention, prior to planting of bulbs 10 of ornamental bulbous plants in the treated furrows, the above-described treatment with tolclofos-methyl and azoxystrobin may be carried out simultaneously with providing furrows in the soil of the cultivation area for ornamental bulbous plants by using a conventional furrow opener.

15 The method of the present invention can be carried out by spraying or injecting tolclofos-methyl and azoxystrobin, either in combination or separately, into the furrows after planting bulbs of ornamental bulbous plants into the furrows provided in a cultivation area for ornamental bulbous plants. In case of 20 treatment with tolclofos-methyl and azoxystrobin after planting the bulbs into the furrows, tolclofos-methyl and azoxystrobin may be independently or together in the formulated form of an oil solution, water-dispersible powders, water-dispersible granules, dust powders, granules, an emulsion or a flowable, or 25 a water dilution thereof.

After the above-mentioned steps of treating the furrows and planting bulbs of ornamental bulbous plants, the bulbs are covered with soil.

Such method may provide efficient cultivation of ornamental 30 bulbous plants by controlling plant diseases caused by soil-borne pathogens that may occur during growth of the plants.

In the present invention, although the treatment amount of tolclofos-methyl and azoxystrobin is appropriately determined depending on the species of ornamental bulbous plants 35 and the size of the bulbs, the type and the degree of occurrence of plant diseases, the forms of tolclofos-methyl and 7 azoxystrobin to be used, the timing of the treatment, and weather conditions, the total amount of tolclofos-methyl and azoxystrobin is generally from 1 to 1000 kg, preferably from 10 to 500 kg per 10000 m2 of the cultivation area for ornamental 5 bulbous plants. Or depending on the above-mentioned conditions, the total amount is preferably from 1 to 100kg, more preferably from 1.5 to 50kg per 10000 m2 of the cultivation area for ornamental bulbous plants.

10 Examples

The present invention is further described in detail below by reference, for example, to test examples. However, the present invention is not limited to the following examples.

In the following examples, unless otherwise 15 specified, the term "part" refers to "part by weight."

Initially, reference formulation examples are shown below.

Reference Formulation Example 1

An emulsion is obtained by well mixing 2.5 parts of 20 tolclofos-methyl, 1.25 parts of azoxystrobin, 14 parts of polyoxyethylene styryl phenyl ether, 6 parts of calcium dodecylbenzenesulfonate and 76.25 parts of xylene.

Reference Formulation Example 2 25 A flowable formulation is obtained by mixing 5 parts of tolclofos-methyl, 5 parts of azoxystrobin, 35 parts of a mixture of white carbon and a polyoxyethylene alkyl ether sulfate ammonium salt (weight ratio 1:1) and 55 parts of water, followed by pulverizing the mixture by wet grinding.

30

Reference Formulation Example 3 A flowable formulation is obtained by mixing 10 parts of tolclofos-methyl, 10 parts of azoxystrobin, 1.5 parts of sorbitan trioleate and 23.5 parts of an aqueous solution 35 containing 2 parts of polyvinyl alcohol, then by pulverizing the mixture by wet grinding, further by adding 45 parts of an 8 aqueous solution containing 0.05 part of Xanthan gum and 0.1 part of aluminum magnesium silicate to the mixture, still further by adding 10 parts of propylene glycol, followed by blending the resulting mixture by stirring.

5

Reference Formulation Example 4 A flowable formulation is obtained by mixing 20 parts of tolclofos-methyl, 5 parts of azoxystrobin, 1.5 parts of sorbitan trioleate and 28.5 parts of an aqueous solution 10 containing 2 parts of polyvinyl alcohol, then by pulverizing the mixture by wet grinding, further by adding 45 parts of an aqueous solution containing 0.05 part of Xanthan gum and 0.1 part of aluminum magnesium silicate to the mixture, still further by adding 10 parts of propylene glycol, followed by 15 blending the resulting mixture by stirring.

Reference Formulation Example 5

Forty parts of tolclofos-methyl, 5 parts of azoxystrobin, 5 parts of propylene glycol (manufactured by 20 Nacalai Tesque, INC.), 5 parts of Soprophor FLK (manufactured by Rhodia Nicca, Ltd.), 0.2 part of an anti-form C emulsion (manufactured by Dow Corning Corporation), 0.3 part of proxel GXL (manufactured by Arch Chemicals, Inc.) and 49.5 parts of ion-exchange water are mixed to prepare a bulk slurry. To 100 25 parts of the slurry, 150 parts of glass beads (Φ=1 mm) are added, and the slurry is ground for 2 hours while it is cooled with coolant water. After the grinding, the glass beads are removed by filtration, thereby obtaining a flowable formulation.

30 Reference Formulation Example 6

An AI premix is obtained by mixing 50 parts of

tolclofos-methyl, 0.5 part of azoxystrobin, 38.5 parts of NN kaolin clay (manufactured by Takehara Chemical Industrial Co., Ltd.), 10 parts of Morwet D425 and 1.5 parts of Morwer EFW

35 (manufactured by Akzo Nobel N.V.). The premix is pulverized with a jet mill to obtain powder.

9

Reference Formulation Example 7 A granule was obtained by well grinding and mixing 1 part of tolclofos-methyl, 1 part of azoxystrobin, 1 part of 5 synthetic hydrated silicon oxide, 2 parts of calcium lignin sulfonate, 30 parts of bentonite and 65 parts of kaolin clay, and then by adding water to well knead the mixture, followed by granulation and drying.

10 Reference Formulation Example 8 A water-dispersible powder is obtained by well grinding and mixing 40 parts of tolclofos-methyl, 1 part of azoxystrobin, 3 parts of calcium lignin sulfonate, 2 parts of sodium lauryl sulfate and 54 parts of synthetic hydrated silicon 15 oxide.

Reference Formulation Example 9 A powder is obtained by well grinding and mixing 1 part of tolclofos-methyl, 10 parts of azoxystrobin, 79 parts of 20 kaolin clay, and 10 parts of talc.

Reference Formulation Example 10

An emulsion is obtained by well mixing 2 parts of tolclofos-methyl, 0.25 part of azoxystrobin, 14 parts of 25 polyoxyethylene styryl phenyl ether, 6 parts of calcium dodecylbenzenesulfonate and 77.75 parts of xylene.

Reference Formulation Example 11 A flowable formulation is obtained by mixing 10 parts 30 of tolclofos-methyl, 2.5 parts of azoxystrobin, 1.5 parts of sorbitan trioleate and 30 parts of an aqueous solution containing 2 parts of polyvinyl alcohol, then by pulverizing the mixture by wet grinding, further by adding 47.5 parts of an aqueous solution containing 0.05 part of Xanthan gum and 0.1 35 part of aluminum magnesium silicate to the mixture, still 10 further by adding 10 parts of propylene glycol, followed by blending the resulting mixture by stirring.

Reference Formulation Example 12 5 A granule was obtained by well grinding and mixing 20 parts of tolclofos-methyl, 1 part of azoxystrobin, 1 part of synthetic hydrated silicon oxide, 2 parts of calcium lignin sulfonate, 30 parts of bentonite and 47 parts of kaolin clay, and then by adding water to well knead the mixture, followed by 10 granulation and drying.

Reference Formulation Example 13 A water-dispersible powder is obtained by well grinding and mixing 5 parts of tolclofos-methyl, 10 parts of 15 azoxystrobin, 5 parts of calcium lignin sulfonate, 3 parts of sodium lauryl sulfate and 77 parts of synthetic hydrated silicon oxide.

Next, test examples are shown below.

20

Test Example 1 A test solution is prepared by mixing tolclofos-methyl 50% (w/v) flowable (Product name: Rizolex® Flowable; Sumitomo Chemical Co. Ltd.) and azoxystrobin 20% (w/w) flowable 25 (Product name: Amistar®20 Flowable; Syngenta Japan K.K.) in water.

Filling a planter (size: 35cm χ 65cm, depth 26 cm) with soil, furrows each with width 7cm, depth 10 cm and length 70 cm are made. One hundred (100) ml of test solution is injected to 30 each furrow, per length 70 cm. The amount of the injected compound is shown in Table 1. Then 5 bulbs of tulip (variety "Angelique") are planted in each of the treated furrows and then covered with soil, followed by being cultured in a glasshouse.

(These tests are conducted twice, which are the treated 35 plots).

11

On the other hand, cultivation is carried out as described above, except that the test solution is not injected into furrows (the untreated plots).

After a predetermined time period, each of the 5 treated and non-treated plots is observed for the presence or absence of germination and the emergence of disease in the sprouts. The percentage disease incidence is calculated according to Equation 1 for each of the treated and untreated plots, and the disease control value is calculated according to 10 Equation 2 for the treated plot.

Percentage disease incidence = (number of non-germinated bulbs + number of diseased plants)/ (total number of bulbs) x 100 (Equation 1) 15

Disease control value = (A-B)/ A x 100 (Equation 2), A: Percentage disease incidence in the untreated plot; B: Percentage disease incidence in the untreated plot 20

The results show that plant diseases are controlled in the treated plot.

Table 1

Applied Amount of Applied Amount of

Test No. Tolclofos-Methyl Azoxystrobin [a.i. kg/10000m2] [a.i. kg/10000m2] ï 3Ö 175 2 15 ΓΤ5 3 Π5 15 4 Ö Ö 25 Test Example 2 A test solution was prepared by mixing tolclofos-methyl 50% (w/v) flowable (Product name: Rizolex® Flowable;

Sumitomo Chemical Co. Ltd.) and azoxystrobin 20% (w/w) flowable 12 (Product name: Amistar®20 Flowable; Syngenta Japan K.K.) in water. Filling a planter (size: 35cm χ 65cm, depth 26 cm) with soil, furrows each with width 7cm, depth 10 cm and length 70 cm were made. One hundred (100) ml of test solution was injected 5 to each furrow, per length 70 cm. The amount of the injected compound was shown in Table 2. Then 5 bulbs of tulip (variety "Angelique") were planted in each of the treated furrows and then covered with soil, followed by being cultured in a glasshouse.

10 (These tests are conducted twice, which are the treated plots).

On the other hand, cultivation was carried out as described above, except that the test solution was not injected into furrows (the untreated plots).

15 After a predetermined time period, about 8 weeks, each of the treated and non-treated plots was observed for the presence or absence of germination and the emergence of disease in the sprouts. The percentage disease incidence was calculated according to Equation 1, shown in Test Example 1, for each of 20 the treated and untreated plots, and the disease control value was calculated according to Equation 2, shown in Test Example 1, for the treated plot.

The results are shown in Table 2.

Table 2

Applied Applied

Amount of Amount of Percentage Disease

Test

Tolclofos-Methyl Azoxystrobin disease control

No.

[a.i. [a.i. incidence value kg/10000m2] kg/10000m2] 5 3Ö ΓΤ5 2Ö 71.4 6 15 ΪΤ5 Ö 1ÖÖ 7 175 15 Ö 1ÏÏÖ 8 Ö Ö 70 25 13

Test Example 3 A test solution is prepared by mixing tolclofos-methyl 50% (w/v) flowable (Product name: Rizolex® Flowable;

Sumitomo Chemical Co. Ltd.) and azoxystrobin 20% (w/w) flowable 5 (Product name: Amistar®20 Flowable; Syngenta Japan K.K.) in water.

Filling a planter (size: 35cm * 65cm, depth 26 cm) with soil, furrows each with width 7cm, depth 10 cm and length 70 cm are made. One hundred (100) ml of test solution is injected to 10 each furrow, per length 70 cm. The amount of the injected compound is shown in Table 1. Then 5 bulbs of lily (variety "Party Diamond") are planted in each of the treated furrows and then covered with soil, followed by being cultured in a glasshouse.

15 (These tests are conducted twice, which are the treated plots) .

20 After a predetermined time period, each of the treated and non-treated plots is observed for the presence or absence of germination and the emergence of disease in the sprouts. The percentage disease incidence is calculated according to Equation 1, shown in Test Example 1, for each of 25 the treated and untreated plots, and the disease control value is calculated according to Equation 2, shown in Test Example 1, for the treated plot.

The results show that plant diseases are controlled in the treated plot.

30 35 14

Table 3

Applied Amount of Applied Amount of

Test No. Tolclofos-Methyl Azoxystrobin [a.i. kg/10000m2] [a.i. kg/10000m2] 9 30 Γ5 Ï0 15 ΓΤ5 Π Π5 15 Ï2 Ö Ö

Test Example 4 A test solution was prepared by mixing tolclofos-5 methyl 50% (w/v) flowable (Product name: Rizolex® Flowable;

Sumitomo Chemical Co. Ltd.) and azoxystrobin 20% (w/w) flowable (Product name: Amistar®20 Flowable; Syngenta Japan K.K.) in water.

Filling a planter (size: 35cm * 65cm, depth 26 cm) 10 with soil, furrows each with width 7cm, depth 10 cm and length 70 cm were made. One hundred (100) ml of test solution was injected to each furrow, per length 70 cm. The amount of the injected compound was shown in Table 2. Then 5 bulbs of lily (variety "Party Diamond") were planted in each of the treated 15 furrows and then covered with soil, followed by being cultured in a glasshouse.

(These tests are conducted twice, which are the treated plots) .

On the other hand, cultivation was carried out as 20 described above, except that the test solution was not injected into furrows (the untreated plots).

After a predetermined time period, about 15 weeks, each of the treated and non-treated plots was observed for the presence or absence of germination and the emergence of disease 25 in the sprouts. The percentage disease incidence was calculated according to Equation 1, shown in Test Example 1, for each of the treated and untreated plots, and the disease control value 15 was calculated according to Equation 2, shown in Test Example 1, for the treated plot.

The results are shown in Table 4.

5 Table 4

Applied Applied

Perce Di

Amount of Amount of

Test ntage sease

Tolclofos-Methyl Azoxystrobin

No. disease control [a. i . [a. i .

. _ incidence value kg/10000m2] kg/10000m2] "Ï3 15 Π5 40 60~ H Ö Ö 80

Test Example 5 A test solution is prepared by mixing tolclofos- methyl 50% (w/v) flowable (Product name: Rizolex® Flowable; 10 Sumitomo Chemical Co. Ltd.) and azoxystrobin 20% (w/w) flowable (Product name: Amistar®20 Flowable; Syngenta Japan K.K.) in water.

Filling a planter (size: 35cm * 65cm, depth 26 cm) with soil, furrows each with width 7cm, depth 10 cm and length 15 70 cm are made. One hundred (100) ml of test solution is injected to each furrow, per length 70 cm. The amount of the injected compound is shown in Table 1. Then 5 bulbs of gladiolus (variety "Cayenne") are planted in each of the treated furrows and then covered with soil, followed by being cultured 20 in a glasshouse.

(These tests are conducted twice, which are the treated plots) .

On the other hand, cultivation is carried out as described above, except that the test solution is not injected 25 into furrows (the untreated plots).

After a predetermined time period, each of the treated and non-treated plots is observed for the presence or absence of germination and the emergence of disease in the 16 sprouts. The percentage disease incidence is calculated according to Equation 1, shown in Test Example 1, for each of the treated and untreated plots, and the disease control value is calculated according to Equation 2, shown in Test Example 1, 5 for the treated plot.

The results show that plant diseases are controlled in the treated plot.

Table 5

Applied Amount of Applied Amount of

Test No. Tolclofos-Methyl Azoxystrobin [a.i. kg/10000m2] [a.i. kg/10000m2] 15 3Ö Π5 Ï6 15 175 17 175 15 18 Ö Ö 10

Test Example 6 A test solution was prepared by mixing tolclofos-methyl 50% (w/v) flowable (Product name: Rizolex® Flowable;

Sumitomo Chemical Co. Ltd.) and azoxystrobin 20% (w/w) flowable 15 (Product name: Amistar®20 Flowable; Syngenta Japan K.K.) in water.

Filling a planter (size: 35cm χ 65cm, depth 26 cm) with soil, furrows each with width 7cm, depth 10 cm and length 70 cm were made. One hundred (100) ml of test solution was injected 20 to each furrow, per length 70 cm. The amount of the injected compound was shown in Table 2. Then 5 bulbs of gladiolus (variety "Cayenne") were planted in each of the treated furrows and then covered with soil, followed by being cultured in a glasshouse.

25 (These tests are conducted twice, which are the treated plots) .

17

After a predetermined time period, about 7 weeks, 5 each of the treated and non-treated plots was observed for the presence or absence of germination and the emergence of disease in the sprouts. The percentage disease incidence was calculated according to Equation 1, shown in Test Example 1, for each of the treated and untreated plots, and the disease control value 10 was calculated according to Equation 2, shown in Test Example 1, for the treated plot.

The results are shown in Table 6.

Table 6

Applied Applied

Amount of Amount of Percentage Disease

Test

Tolclofos-Methyl Azoxystrobin disease control NO.

(a.i. [a.i. incidence value kg/10000m2] kg/10000m2] ï ÏÖ~ 15 1.5 0 9 0 2 0 0 70 0 15 Test Example 7 A test solution is prepared by mixing tolclofos-methyl 50% (w/v) flowable (Product name: Rizolex® Flowable;

Sumitomo Chemical Co. Ltd.) and azoxystrobin 20% (w/w) flowable (Product name Amistar®20 Flowable; Syngenta Japan K.K.) in 20 water.

Filling a planter (size: 35cm * 65cm, depth 26 cm) with soil, furrows each with width 7cm, depth 10 cm and length 70 cm are made. One hundred (100) ml of test solution is injected to each furrow, per length 70 cm. The amount of the injected 25 compound is shown in Table 1. Then 5 bulbs of hyacinth (variety "Odysseus") are planted in each of the treated furrows 18 and then covered with soil, followed by being cultured in a glasshouse.

(These tests are conducted twice, which are the treated plots) .

5 On the other hand, cultivation is carried out as described above, except that the test solution is not injected into furrows (the untreated plots).

After a predetermined time period, each of the treated and non-treated plots is observed for the presence or 10 absence of germination and the emergence of disease in the sprouts. The percentage disease incidence is calculated according to Equation 1, shown in Test Example 1, for each of the treated and untreated plots, and the disease control value is calculated according to Equation 2, shown in Test Example 1, 15 for the treated plot.

The results show that plant diseases are controlled in the treated plot.

Table 7

Applied Amount of Applied Amount of

Test No. Tolclofos-Methyl Azoxystrobin [a.i. kg/10000m2] [a.i. kg/100Q0m2] 21 30 571 22 15 ÏT5 23 175 15 24 Ö Ö 20

Test Example 8 A predetermined concentration of a test solution was prepared by mixing tolclofos-methyl 50% (w/v) flowable (Product name: Rizolex® Flowable; Sumitomo Chemical Co. Ltd.) and 25 azoxystrobin 20% (w/w) flowable (Product name Amistar®20

Flowable; Syngenta Japan K.K.) in water.

Filling a planter (size: 35cm χ 65cm, depth 26 cm) with soil, furrows each with width 7cm, depth 10 cm and length 70 cm 19 were made. One hundred (100) ml of test solution was injected to each furrow, per length 70 cm. The amount of the injected compound was shown in Table 2. Then 5 bulbs of hyacinth (variety "Odysseus") were planted in each of the treated furrows 5 and then covered with soil, followed by being cultured in a glasshouse .

(These tests are conducted twice, which are the treated plots).

On the other hand, cultivation was carried out as 10 described above, except that the test solution was not injected into furrows (the untreated plots).

After a predetermined time period, about 15 weeks, each of the treated and non-treated plots was observed for the presence or absence of germination and the emergence of disease 15 in the sprouts. The percentage disease incidence was calculated according to Equation 1, shown in Test Example 1, for each of the treated and untreated plots, and the disease control value was calculated according to Equation 2, shown in Test Example 1, for the treated plot.

20 The results are shown in Table 8.

Table 8

Applied Applied

Amount of Amount of Percentage Disease

Test

Tolclofos-Methyl Azoxystrobin disease control

No.

[a.i. [a.i. incidence value kg/10000m2] kg/10000m2] "~25 15 ÏT5 Ö 1ÏÏÖ ~26 Ö Ö 4Ö 1039387

Claims

Method for controlling plant diseases in an ornamental bulb plant, comprising the following steps: treating, with an effective amount of tolclophos-methyl and azoxystrobin, from the front in the soil of a growing area for the ornamental bulb crop with a depth of 1 to at 40 cm and a width from 1 to 50 cm; and planting bulbs of the ornamental bulb crop in the furrows. 10

The method as set forth in claim 1, wherein the total amount of tolclophos-methyl and azoxystrobin is from 1 to 1000 kg per 10,000 m2 of an ornamental bulb growing area. 15

The method as set forth in claim 1 or claim 2, wherein the amount of azoxystrobin is from 1 to 1000 parts by weight relative to 100 parts by weight of tolclophos-methyl.

The method as set forth in any one of claims 1 to 3, wherein the ornamental bulb is selected from the group consisting of: tulip, lily, gladiolus and hyacinth. 25

The method as set forth in any one of claims 1 to 3, wherein the ornamental bulb crop consists of tulips.

A method for growing an ornamental bulb plant, comprising the steps of: treating, with tolclophos-methyl and azoxystrobin in an amount effective for controlling plant diseases, from the front, with a depth of 1 to 40 cm 35 and a width of 1 to 50 cm, in the bottom of a growing area for the ornamental bulb crop; 1039387 planting bulbs of the ornamental bulb; and then filling with soil from the front where the bulbs are planted.

The method as set forth in claim 6, wherein the total amount of tolclophos-methyl and azoxystrobin is from 1 to 1000 kg per 10,000 m2 of an ornamental bulb growing area.

The method as set forth in claim 6 or claim 7, wherein the amount of azoxystrobin is from 1 to 1000 parts by weight relative to 100 parts by weight of tolclophos-methyl.

The method as set forth in any one of claims 6 to 8, wherein the ornamental bulb is a plant selected from the group consisting of: tulip, lily, gladiolus and hyacinth.

The method as set forth in any one of claims 6 to 8, wherein the ornamental bulb consists of tulips. 25 30 35 1 03 9 387