CN107114392A - Bio-insecticidal composition comprising destruxins - Google Patents
Bio-insecticidal composition comprising destruxins Download PDFInfo
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- CN107114392A CN107114392A CN201710322666.2A CN201710322666A CN107114392A CN 107114392 A CN107114392 A CN 107114392A CN 201710322666 A CN201710322666 A CN 201710322666A CN 107114392 A CN107114392 A CN 107114392A
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- Prior art keywords
- destruxins
- indoxacarb
- bio
- insecticidal composition
- present
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of bio-insecticidal composition for including destruxins, its using destruxins and indoxacarb as principle active component, wherein, the weight ratio of destruxins and indoxacarb is 1:20‑10:1, active component has obvious synergistic effect in the range of aforementioned proportion to various crop insect in the Pesticidal combination；The bio-insecticidal composition of the present invention can be prepared as any one dosage form for being adapted to agriculturally use, and the dosage form is preferably any one in missible oil, water dispersible granules, microemulsion, suspending agent, aqueous emulsion, microcapsule suspending agent；The Pesticidal combination of the present invention is mainly used in the lepidoptera pests such as preventing and treating diamondback moth, beet armyworm, cabbage butterfly, bollworm.
Description
Technical field
The invention belongs to biological pesticide technical field, and in particular to be a kind of biology comprising destruxins and indoxacarb
Pesticidal combination, the bio-insecticidal composition is safe efficient, is widely used in preventing and treating various crop pests.
Background technology
Diamondback moth is a kind of global brassicaceous vegetable primary pest.For a long time, the preventing and treating of diamondback moth it is main according to
Rely in chemical pesticide.A large amount of insecticides are used due to unreasonable, make diamondback moth to the desinsection developed before nearly all nineties
Agent generates the higher resistance to the action of a drug.Since the nineties, external agricultural chemicals company develops several specific to diamondback moth new
Type insecticide, such as AVM, Frontline, organic phosphates, but these insecticides are expensive, greatly increase and prevent and treat into
This, and these insecticides are solely used, also lead to these insecticides and develop immunity to drugs.
Indoxacarb, English name is indoxacarb, is by the new height of du pont company's success Kai Fa oxadiazine classes
Low toxic pesticide is imitated, is a kind of broad-spectrum highly efficient insecticide, and cross resistance is not present with other insecticides, is to substitute organophosphorus insecticidal
One of preferable kind of worm agent.Its mechanism of action is different from current various insecticides, is sodium-ion channel inhibitor, causes target
Mark insect coordination, paralysis, final death.Be mainly used in preventing and treating vegetables, many Species of Lepidopterous Insect Pests such as diamondback moth of fruit tree and cotton,
Beet armyworm, cabbage caterpillar, prodenia litura etc., but its absorbability in crop body is very poor, substantially inconductivity.
Destruxins, also known as destruxin or destruction element, the ring contracting carboxylic peptide being made up of 5 amino acid and 1 carboxylic acid
Element, is a kind of secondary metabolite that green muscardine fungus produces in metabolic process, to Lepidoptera, Homoptera, Diptera coleoptera etc.
20 various pests have action of contace poison and Antifeedant Effects；Destruxins has the characteristics of efficient, low toxicity, prevention effect are stable, but
Long-term use also inevitably causes the generation of pest resistance to insecticide.
In order to reduce the cost accounting of vegetable-crop pest-insect, delay insect to the resistance development of existing excellent pesticidal agent, it is to avoid single
One using the resistance to the action of a drug caused by insecticide, is badly in need of exploitation and is directed to the efficient of diamondback moth, low toxicity, cheap chemical compounding system
Agent.The compounding of different pesticide species, is the method for overcoming or delaying pest resistance to insecticide to produce.Different farm chemical ingredients are compounded,
According to practical application effect, to judge that compounding is synergy, addition or antagonism.Present inventor passes through substantial amounts of room
Interior screening active ingredients and field control effectiveness test, be found surprisingly that will have to various pests after destruxins and indoxacarb compounding it is notable
Synergistic effect, and there is no at present about destruxins and indoxacarb compounding relevant report.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of with synergistic function, insecticidal effect
Good bio-insecticidal composition, the Pesticidal combination is very safe to world insect, the low-residual in crop, is that can be used for nothing
The favourable agents of control of insect in the production of public hazards agricultural product.
Another object of the present invention is to provide the application of the Biocidal composition pesticide, in particular for preventing and treating vegetable
The agriculture and garden crop pest such as dish, beet, fruit tree, gardening, flowers, such as diamondback moth, beet armyworm, cabbage butterfly, bollworm squama
Wing mesh insect.
To reach above-mentioned purpose, the present invention is adopted the following technical scheme that：
A kind of bio-insecticidal composition, its active component includes destruxins and indoxacarb, wherein, destruxins and indoxacarb
Weight ratio is 1:20-10:1.
Further, the weight ratio of the destruxins and indoxacarb is 1:10-5:1.
Further, the weight percentage of active component is 1-80%, preferably 10- in Pesticidal combination of the invention
50%, remaining is auxiliary element conventional in Pesticide formulation, such as surfactant, filler and/or carrier.
It is preferred that, bio-insecticidal composition of the invention is prepared as suitable agricultural by being aided with above-mentioned conventional auxiliary element
On any one dosage form for using, the dosage form is preferably missible oil, water dispersible granules, microemulsion, suspending agent, water and milk
Any one in agent, microcapsule suspending agent, it is specifically used in the formulation form to be processed can be according to using object, use environment
Do not selected and prepared by conventional method on an equal basis.
It is preferred that, in Pesticidal combination of the invention can also contain other pesticide activity components, for example can be insecticide,
Bactericide, fertilizer, plant growth regulator, synergist, biostimulant etc., or can be used in combination with them.
Further, the destruxins is containing green pure substance or mixture by rhzomorph A, B, C, D, E, the indoxacarb
For pure substance or mixture containing indoxacarb, above-mentioned pesticide activity component can be commercially available by conventional commercial means.
Further, the percentage in embodiment refers both to percentage by weight, and the destruxins refers both to effective dose, the indenes
Worm prestige it is actual prepare when according to contained by active compound converted.
Compared with prior art, Biocidal composition pesticide of the invention has the beneficial effect that：
（1）There is obvious synergistic function, compared with single dose, Ke Yixian between the composition pesticide active component of the present invention
Write reduction pesticide activity component usage amount, threat of the reduction to environment；
（2）The active component mechanism of action is different in the composition pesticide of the present invention, and both can increase target site at compounding, gram
Take and/or delay the drug-fast generation of pathogen；
（3）The composition pesticide of the present invention, which is expanded, prevents spectrum, there is well simultaneous to control effect simultaneous various pests.
Embodiment
Below by embodiment, the present invention will be further described, it should be understood that is prepared described in the embodiment of the present invention
Method is only the description of the invention, and be should be in no way restrictive, to the present invention under the concept thereof of the present invention
The simple modifications of preparation method can be seen as belonging to the scope of protection of present invention, and the scope of protection of present invention is to weigh
Sharp claim is defined.
First, example of formulations
1st, embodiment 1：33% destruxins indoxacarb wettable powder（1:10）
Destruxins 3%
Indoxacarb 30%
Neopelex 5%
Sodium lignin sulfonate 6%
White carbon 15%
Diatomite surplus
The preparation method of the wettable powder is：By above-mentioned formula by active component, wetting agent, dispersant and filler according to
Ratio is well mixed, after ultra-fine pulverizer disintegrating, you can wettable powder of the present invention is made.
2nd, embodiment 2:30% destruxins indoxacarb water dispersible granule（1:5）
Destruxins 5%
Indoxacarb 25%
Dialkyl group Succinate sodium sulfonate salt 6%
Polycarboxylate 5%
Ammonium sulfate 4%
Starch 5%
Bentonite surplus
The preparation method of the water dispersible granules is：Destruxins and indoxacarb are added in carrier by above-mentioned formula, Ran Houjia
Enter wetting agent, dispersant, disintegrant, binding agent mixing, appropriate water is added after air-flow crushing, through mediating, granulating, dry,
Screening can be prepared by water dispersible granules of the present invention.
3rd, embodiment 3:36% destruxins indoxacarb suspending agent（1:1）
Destruxins 18%
Indoxacarb 18%
AEO 5%
Alkylnaphthalene sulfonate formaldehyde condensation products 6%
Aluminium-magnesium silicate 3%
C10Fatty alcohol 1.4%
OIT 0.6%
Urea 3%
Deionized water surplus
The preparation method of the suspending agent is：By wetting agent, dispersant, defoamer, thickener, antifreeze, preservative through at a high speed
Shear-mixed is uniform, adds destruxins and indoxacarb, ball milling more than 2 hours in ball mill so that preparation particle diameter is micro- 5
Rice is following, adds deionized water, further stirring, you can suspending agent of the present invention is made.
4th, embodiment 4:18% destruxins indoxacarb aqueous emulsion（5:1）
Destruxins 15%
Indoxacarb 3%
Aliphatic alcohol polyoxyvinethene phosphate 4%
Sodium lignin sulfonate 4%
Isobutanol 3%
1-METHYLPYRROLIDONE 15%
Organic silicone 4%
Glycerine 3%
Deionized water surplus
The preparation method of the aqueous emulsion is：Destruxins and indoxacarb, solvent, emulsifying agent and cosolvent are added by above-mentioned formula
Together, it is made to be dissolved as uniform oil phase；Then other insecticides adjuvants such as part water, antifreeze are mixed to be formed
Even aqueous phase；Oil phase is added into aqueous phase while high-speed stirred in a kettle., slowly added water until reaching phase inversion point, unlatching is cut
Cut machine and carry out high speed shear, and add remaining water, shear about 30 minutes, you can aqueous emulsion of the present invention is made.
5th, embodiment 5:24% destruxins indoxacarb suspending agent（3:5）
Destruxins 9%
Indoxacarb 15%
Dibutyl naphthalenesulfonic acid salt 4%
EO-PO block polyethers 5%
Xanthans 3%
Silicone oil 1.4%
OIT 0.6%
Glycerine 3%
Deionized water surplus
The preparation method of the suspending agent is：By wetting agent, dispersant, defoamer, thickener, antifreeze, preservative through at a high speed
Shear-mixed is uniform, adds destruxins and indoxacarb, ball milling more than 2 hours in ball mill so that preparation particle diameter is micro- 5
Rice is following, adds deionized water, further stirring, you can suspending agent of the present invention is made.
2nd, Toxicity Determination
1st, subjects：The instar larvae of wild cabbage diamondback moth 3；
2nd, test method：Reference《People's Republic of China (PRC) agricultural industry criteria NY-T1154.7-2006》, wild cabbage will be picked up from
Diamondback moth through the generation of indoor feeding 5, select 3 consistent instar larvaes of growth, be connected to the culture for the cm of diameter 9 for putting wild cabbage dish leaf
Ware, connects 10 third-instar larvaes and makees Anesthetic preconditioning, sprayed with Potter spray towers under 50PST pressure, one is sprayed every time per ware
Individual ware, per the mL of ware spraying liquid 1, blank control spray equivalent clear water.Each processing sets 6 concentration gradients, is repeated 4 times.Will processing
The culture dish crossed is put into 25 DEG C, 12H/12H illumination boxs and cultivated, and checks life or death borer population within 72 hours, calculates the death rate.With
DPS data processing softwares carry out statistical analysis, calculate the LC of each medicament50, then calculate co-toxicity coefficient according to the abundant methods of Sun Yun
(CTC), result of the test is as shown in table 1.
As CTC≤80, composition shows as antagonism；
As 80 ＜ CTC ＜ 120, composition shows as summation action；
As CTC >=120, then composition shows as synergistic effect.
As shown in table 1, as shown in Table 1, destruxins and indoxacarb are 1 for Toxicity Determination result:20-10:It is right in the range of 1
The co-toxicity coefficient of wild cabbage diamondback moth preventing and treating shows as synergistic function more than 120；And outside the above range, experiment
It is in processing to show as summation action more.Wherein, when both compound proportions are 1:10-5:When 1, co-toxicity coefficient is all higher than 150, collaboration
Synergistic effect is relative to be become apparent from；And when the compound proportion of destruxins and indoxacarb is 3:When 5, synergistic function is most notable,
Co-toxicity coefficient is up to 219.51.
3rd, field control effectiveness test
In order to further verify the practical application effect of bio-insecticidal composition of the invention, using 1-5's of the embodiment of the present invention
Pesticidal preparations carry out preventing and treating wild cabbage diamondback moth field control effectiveness test, with 0.5% destruxins suspending agent（Carried by Agricultural University Of South China
For）, 5% indoxacarb suspending agent（Purchased from Guangxi Tianyuan Biochemical Co., Ltd.）.
Test method：Experiment is arranged on the truck garden progress of Guangzhou, Guangdong Huadu District somewhere, and experiment is using conventional
Spraying is watered, spraying equipment is WF-16 type knapsack hand sprayers, and shower nozzle is single fan-shaped spray fog-spray nozzle, and operating pressure is
0.2-0.4 Mpa, spray amount is 0.36-0.48 L/min, carries out conventional spraying, makes every effort to uniform thoughtful during spray, sweet during dispenser
Blue vegetable plot diamondback moth is larva occurance peak（Based on 2-3 ages）.Sampled respectively at pressing 5 points of diagonal in every cell before dispenser,
Choosing for every has 2-3 plant of Beet Armyworm Eggs and a wild cabbage of larva, and beet exigua larvae head number is gone up in probability demarcation strain, in this, as applying
Insect population before medicine, in the diamondback moth larvae number that survival is remained 3 after dispenser, in investigation fixed point strain in 7,14 days, is subtracted with correcting insect population
Rate is as prevention effect, and result of the test is as shown in table 2.Drug effect computational methods are as follows：
As shown in table 2, composition pesticide of the invention is 15 g a.i./hm in application dosage to field control effectiveness test result2Can have
The generation for preventing and treating wild cabbage diamondback moth of effect, compared with the control, 1-5 of embodiment of the present invention composition pesticide prevention effect are more preferable,
Lasting period is longer.7 after the especially composition pesticide preparation of embodiment 5, medicine, the preventive effect of 14 days is more than 90%, hence it is evident that be better than
Comparison medicament.In addition, not finding the composition pesticide preparation of the present invention during experiment has poisoning generation to cole crops, show this
The composition pesticide security of invention is also preferable, is worth the popularization and application in production.
Claims (7)
1. a kind of bio-insecticidal composition, it is characterised in that active component includes destruxins and indoxacarb.
2. bio-insecticidal composition according to claim 1, it is characterised in that the weight of the destruxins and indoxacarb
Than for 1:20-10:1.
3. bio-insecticidal composition according to claim 2, it is characterised in that the weight of the destruxins and indoxacarb
Than being preferably 1:10-5:1, more preferably 3:5.
4. the bio-insecticidal composition according to any one of claim 1-3 claim, it is characterised in that the biology
Pesticidal combination can also contain other agricultural active compositions.
5. the bio-insecticidal composition described in as requested 4, it is characterised in that other agricultural active compositions are selected from desinsection
One or more combination in agent, bactericide, fertilizer, plant growth regulator, synergist, biostimulant.
6. the bio-insecticidal composition according to claim 1-5 is used for the purposes for preventing and treating crop pests.
7. purposes according to claim 6, it is characterised in that the crop pests be preferably diamondback moth, beet armyworm,
Cabbage butterfly, bollworm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710322666.2A CN107114392A (en) | 2017-05-09 | 2017-05-09 | Bio-insecticidal composition comprising destruxins |
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| CN201710322666.2A CN107114392A (en) | 2017-05-09 | 2017-05-09 | Bio-insecticidal composition comprising destruxins |
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| CN201710322666.2A Pending CN107114392A (en) | 2017-05-09 | 2017-05-09 | Bio-insecticidal composition comprising destruxins |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108496659A (en) * | 2018-03-19 | 2018-09-07 | 叶虹 | The technique that lepidopterous insect pest is prevented using composite bacteria agent |
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2017
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| CN103636678A (en) * | 2013-11-29 | 2014-03-19 | 安徽省化工研究院 | Pesticide compound preparation of microbial fungicide and antibiotic insecticides |
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| CN108496659A (en) * | 2018-03-19 | 2018-09-07 | 叶虹 | The technique that lepidopterous insect pest is prevented using composite bacteria agent |
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