CN101438713A

CN101438713A - Compositional disinsection main agent containing destruxins of Metarhizium anisopliae and chlopyrifos

Info

Publication number: CN101438713A
Application number: CNA2008102200011A
Authority: CN
Inventors: 胡琼波; 任顺祥
Original assignee: South China Agricultural University
Current assignee: South China Agricultural University
Priority date: 2008-12-15
Filing date: 2008-12-15
Publication date: 2009-05-27
Anticipated expiration: 2028-12-15
Also published as: CN101438713B

Abstract

The invention discloses a pesticidal main agent compounding destruxin and chlorpyrifos, and the mass ratio between the destruxin and the chlorpyrifos is 5 to 1 : 5 to 9; and the invention also discloses a pesticide prepared by adopting the main agent. The invention adopts the highly effectively, secure and broad-spectrum azotic heterocyclic organic phosphorus insecticide and acaricide chlorpyrifos to be compounded with the insect pathogenic fungus destruxin with remarkable pesticidal effect, and the preferable mixed proportion between the destruxin and the chlorpyrifos is determined through experiments, therefore, a new compound pesticide is provided. The pesticide has a completely new function mechanism different form the prior pesticide, has an excellent pesticidal effect particularly in poisoning and killing prodenia litura with serious harms, and meanwhile is environment-friendly.

Description

The desinsection host and the insecticide of a kind of destruxins and chlorpyrifos mixture

Technical field

The invention belongs to biological pesticide technical field, relate to a kind of novel destruxins and the desinsection host and the insecticide of chlorpyrifos mixture.

Background technology

Prodenia litura (Spodoptera litura) is the important pest of a kind of universal omnivorousness, and the frequency in China's big generation in many areas obviously improved in the last few years, and harm increases the weight of.Because the long-term chemical pesticide control that uses, cause this worm that insecticides such as organochlorine, organic phosphor, carbamate and pyrethroid have been produced pesticide resistance in various degree, the most conventional effect of chemical control significantly descends, and can not effectively control its harm, the control difficulty.

Chlopyrifos is the nitrogen heterocyclic ring organophosphorus insecticidal/acaricidal agent of a kind of efficient, safety and wide spectrum, insect had tag, stomach toxicity and fumigation action, can be used on numerous field crops such as water paddy and wheat class, corn, soybean, peanut, cotton, fruit tree, tea tree, sugarcane, vegetables and flowers and the economic crops underground insect, animal parasite also being had the better prevention effect.Because it is relatively low to the person poultry toxicity, insecticidal spectrum is wide, and preventive effect is good, as one of important agricultural chemicals of preventing and treating pest, is used widely in the world, and its sales volume occupies the insecticide first always, contains for a long time not wane.After China forbade sale and uses 5 kinds high malicious organophosphorus insecticides such as acephatemet, parathion, parathion-methyl, nuvacron, phosphamidon from January 1st, 2007 especially, chlopyrifos has been classified the important kind that replaces high-toxic pesticide as, and the recommended special pesticide miticide that is used for non-polluted farm product production.The same with existing other insecticides, if long-term the use with a kind of insecticide causes the pest resistance to insecticide development easily, adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, and therefore, exploitation Compositional type insecticide is significant.

Though the chlorpyrifos mixture agent of China's registration is many, for example chlopyrifos and pyrethroid, carbamates, organic phosphates, antibiotics and growth regulator class principal item all have mix preparation, but insect is constantly development to the pesticide resistance of agricultural chemicals, and Recompounded pesticide has also constantly been proposed new demand for development.

Destruxins (destruxin) is an insect pathogenic fungus---the cyclic depsipeptides antibiotic that green muscardine fungus Metarhiziumanisopliae var.anisopliae produces.Destruxins has significant insecticidal effect, and has the brand-new mechanism of action that is different from existing insecticide, mainly acts on the hemolymph immune system of insect, makes it to lose the immune defense ability.Simultaneously, destruxins is easily degraded, noresidue, and toxicity is low, has eco-friendly characteristics.

Do not see at present the report of composite dose of chlopyrifos and destruxins, development chlopyrifos and destruxins compounded pesticides have great importance to pest control, developing agricultural production, have important economic worth in particular for chlopyrifos of control prodenia litura and composite dose of destruxins.

Summary of the invention

An object of the present invention is to overcome the deficiencies in the prior art, the desinsection host of a kind of destruxins and chlorpyrifos mixture at first is provided, by continuous experimental study, find the suitable compound proportion of destruxins and chlopyrifos, obtain a kind of Compositional type desinsection host of efficient, low toxicity.

Another object of the present invention is to be on the basis of above-mentioned desinsection host, further determines the proportioning of host and assistant agent, prepares good disinsection effect and the insecticide stable, that the practical application generalization is very strong.

Purpose of the present invention is achieved through the following technical solutions:

The desinsection host of a kind of destruxins and chlorpyrifos mixture is provided, and the mass ratio of described destruxins and chlopyrifos is 5～1: 5～9.

The preferred 5:5 of the mass ratio of described destruxins and chlopyrifos, 2:8 and 1:9.

Described destruxins is any one or the two or more mixture that mixes with any ratio among destruxins A, destruxins B or the destruxins E.

The present invention provides the Compositional type insecticide of destruxins and chlopyrifos simultaneously, comprises above-mentioned host and conventional assistant agent, and by mass percentage, host accounts for 50%, and assistant agent accounts for 50%.

Described compounded pesticides can be prepared into various formulations such as emulsifiable concentrate according to existing routine techniques.

The invention has the beneficial effects as follows:

The invention provides a kind of new compound insecticidal host, the insect pathogenic fungus destruxins that utilizes the nitrogen heterocyclic ring organophosphorus insecticidal/acaricidal agent chlopyrifos of efficient, safety and wide spectrum and have a remarkable insecticidal effect carries out composite, has determined the preferred proportion of the two by a large amount of experiments.Be equipped with the insecticide that assistant agent prepares with above-mentioned host as active ingredient and have the brand-new mechanism of action that is different from existing insecticide, insecticidal effect is remarkable, especially the toxic action at the serious prodenia litura of harm is remarkable, has eco-friendly characteristics simultaneously.

Embodiment

Always further describe the present invention below in conjunction with concrete enforcement.

Embodiment 1 desinsection host of the present invention is to the Toxicity Determination experiment of prodenia litura

During Toxicity Determination, according to the mass ratio of destruxins and chlopyrifos is respectively to measure destruxins and chlopyrifos at 5～1: 5～9, be made into certain density mother liquor with acetone and place refrigerator (4 ℃) standby, all medicaments are all used 0.01% Tween-80 solution dilution during experiment.Described destruxins is any one or the two or more mixture that mixes with any ratio among destruxins A, destruxins B or the destruxins E.Each is handled 3 times and repeats, and each repeats 1 culture dish, each culture dish put 15 three age Spodoptera litura larvae.At first cabbage leaves is cut into the fritter of 6～7cm, immerses soup and take out after about 5 seconds, after indoor drying, again blade is put into the culture dish that is connected to prodenia litura, preserve moisture with wet cotton balls.Every 24h changes cabbage leaves and clears up culture dish behind the medicine.Experiment repeats secondary.Behind medicine 24,48,72h observed and recorded prodenia litura survival condition, calculate lethality, corrected mortality, and the lethal concentration of 50 (LC of 72h after the computing ₅₀), correlation coefficient and regression equation, calculate the co-toxicity coefficient (CTC) of intermixture with reference to the abundant method of Sun Yun.Because experimental technique belongs to this area conventional method, experimental result is not given unnecessary details one by one, and part of test results sees Table 1, by the visible destruxins of table 1 and chlopyrifos with 5:5,2:8, when the 1:9 ratio is mixed, its co-toxicity coefficient reaches 127.87,140.27 and 124.47, and synergistic effect is arranged.

Table 1 destruxins, chlopyrifos and intermixture thereof are to the toxicity test of prodenia litura 3 instar larvaes

Illustrate: co-toxicity coefficient CTC had the effect of subtracting effect less than 80 o'clock between the two; Greater than having synergistic effect at 120 o'clock; It in the time of between 80～120 summation action.

Embodiment 2:50% destruxins and chlopyrifos are tested the Toxicity Determination of prodenia litura with 2: 8 composite insecticide emulsifiable concentrates of mass ratio

Described missible oil is diluted with water to concentration gradient to be measured, and experimental technique is with embodiment 1.Experimental result sees Table 2, and the co-toxicity coefficient of described missible oil reaches 153.69, has obvious synergistic effect.

Table 2 destruxins, the single agent of chlopyrifos and 50% green poison (2: 8) missible oil are to the toxicity test of prodenia litura 3 instar larvaes

Claims

1, the desinsection host of a kind of destruxins and chlorpyrifos mixture, the mass ratio that it is characterized in that described destruxins and chlopyrifos is 5～1:5～9.

2, according to the desinsection host of described destruxins of claim 1 and chlorpyrifos mixture, the mass ratio that it is characterized in that described destruxins and chlopyrifos is 5:5,2:8 or 1:9.

3,, it is characterized in that described destruxins is any one or the two or more mixture that mixes with any ratio among destruxins A, destruxins B or the destruxins E according to the desinsection host of claim 1 or 2 described destruxinses and chlorpyrifos mixture.

4, a kind of insecticide comprises host and assistant agent, it is characterized in that by mass percentage, host accounts for 50%, and assistant agent accounts for 50%; Described host destruxins is the mixture that mix 5～1:5～9 with chlopyrifos according to mass ratio.

5, according to the described insecticide of claim 4, it is characterized in that by mass percentage, host accounts for 50%, assistant agent accounts for 50%; Described host destruxins is the mixture that 5:5,2:8 or 1:9 mix with chlopyrifos according to mass ratio.