CN112852865A - Oaan-1 protein, coding gene and application of related biological material thereof - Google Patents

Abstract

The invention discloses an OaAn-1 protein, a coding gene and application of related biological materials thereof. The OaAn-1 protein is a1) or a2) or a3) protein as follows: a1) the amino acid sequence is protein shown as SEQ ID No.1 or SEQ ID No.2 in the sequence table; a2) the protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown in SEQ ID No.1 or SEQ ID No.2 in the sequence table; a3) protein which has 80% or more than 80% of identity with the amino acid sequence limited by SEQ ID No.1 or SEQ ID No.2 in the sequence table, is derived from rice and has the same biological function. According to the specific embodiment of the invention, the OaAn-1 protein coding genes OaAn-1-CC and OaAn-1-DD are simultaneously knocked out by a genome editing technology, so that the rice with obviously shortened miscanthus length is obtained.

Description

Oaan-1 protein, coding gene and application of related biological material thereof

Technical Field

The invention relates to the technical field of biology, in particular to an OaAn-1 protein, a coding gene and application of related biological materials thereof.

Background

The awn of the rice refers to a needle-shaped body with inverted saw teeth, which is positioned at the top end of a rice glume. Wild rice has longer awns, while cultivated rice shows the character of short awns, even no awns. In the natural growth state, the longer awns help the wild rice seeds to spread and store through the relevant media, facilitating the self-reproduction, and the spiky awns also help to protect the seeds from being eaten by predators. However, too long a length of cultivated rice awns grown in a cultivated state is detrimental to the harvesting, processing and storage of seeds. The tetraploid wild rice Oryza alta has the advantages of large biomass, strong adaptability, strong stress resistance and the like, but because the wild rice Oryza alta is not domesticated, the length of the awns is more than 4cm, the wild rice Oryza alta is not beneficial to field harvest, and meanwhile, the difficulty of rice hulling is increased. Therefore, the method for rapidly domesticating the wild rice awn length has practical value by changing long awn into short awn or awn-free character which is more suitable for production practice of people.

Disclosure of Invention

The invention provides application of OaAn-1 protein in regulating rice awn length or preparation of a product for regulating rice awn length.

The OaAn-1 protein is a1) or a2) or a3) protein as follows:

a1) the amino acid sequence is protein shown as SEQ ID No.1 or SEQ ID No.2 in the sequence table;

a2) the protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown in SEQ ID No.1 or SEQ ID No.2 in the sequence table;

a3) protein which has 80% or more than 80% of identity with the amino acid sequence limited by SEQ ID No.1 or SEQ ID No.2 in the sequence table, is derived from rice and has the same biological function.

Hereinbefore, said substitution and/or deletion and/or addition of one or several amino acid residues is a substitution and/or deletion and/or addition of not more than 10 amino acid residues.

The invention also provides the application of the OaAn-1 coding gene in regulating the rice awn length or the application in preparing rice awn length regulating products,

the OaAn-1 protein coding gene is a DNA molecule shown as c1) or c2) or c3) or c 4):

c1) the coding sequence is a DNA molecule shown in SEQ ID No.3 or SEQ ID No.4 in a sequence table;

c2) the nucleotide sequence is a DNA molecule shown as SEQ ID No.5 or SEQ ID No.6 in the sequence table;

c3) a DNA molecule which has 90 percent or more than 90 percent of identity with the nucleotide sequence limited by c1) or c2), is derived from rice and encodes the OaAn-1 protein;

c4) a DNA molecule which is hybridized with the nucleotide sequence limited by c1) or c2) under strict conditions and codes the OaAn-1 protein.

The nucleotide sequence of the gene encoding the OaAn-1 protein of the present invention can be easily mutated by a person of ordinary skill in the art using known methods, such as directed evolution and point mutation. Those nucleotides which are artificially modified to have 90% or more identity to the nucleotide sequence provided by the present invention are derived from the nucleotide sequence of the present invention and are equivalent to the sequence of the present invention as long as they encode the OaAn-1 protein and are derived from rice. The term "identity" as used herein refers to sequence similarity to a native nucleic acid sequence.

The invention also provides the application of the biological material related to the OaAn-1 protein in regulating rice growth or preparing rice growth regulating products,

the biological material is any one of the following C1) to C6) and D1) to D7):

C1) an expression cassette containing the OaAn-1 protein coding gene;

C2) a recombinant vector containing the gene encoding the OaAn-1 protein, or a recombinant vector containing the expression cassette of C1);

C3) a recombinant microorganism containing a gene encoding said OaAn-1 protein, or a recombinant microorganism containing C1) said expression cassette, or a recombinant microorganism containing C2) said recombinant vector;

C4) a transgenic plant cell line containing the gene encoding the OaAn-1 protein, or a transgenic plant cell line containing the expression cassette of C1);

C5) transgenic plant tissue containing the gene encoding the OaAn-1 protein, or transgenic plant tissue containing the expression cassette of C1);

C6) a transgenic plant organ containing said gene encoding an OaAn-1 protein, or a transgenic plant organ containing C1) said expression cassette;

D1) a nucleic acid molecule that inhibits or reduces the expression of a gene encoding said OaAn-1 protein;

D2) an expression cassette comprising the nucleic acid molecule of D1);

D3) a recombinant vector containing the nucleic acid molecule of D1) or a recombinant vector containing the expression cassette of D2);

D4) a recombinant microorganism containing D1) the nucleic acid molecule, or a recombinant microorganism containing D2) the expression cassette, or a recombinant microorganism containing D3) the recombinant vector;

D5) a transgenic plant cell line comprising D1) the nucleic acid molecule or a transgenic plant cell line comprising the expression cassette of D2);

D6) transgenic plant tissue comprising the nucleic acid molecule of D1) or transgenic plant tissue comprising the expression cassette of D2);

D7) a transgenic plant organ containing D1) the nucleic acid molecule or a transgenic plant organ containing D2) the expression cassette.

The invention also provides a method for shortening the rice miscanthus length, which comprises the steps of reducing the content and/or the activity of the OaAn-1 protein in rice and/or reducing or inhibiting the expression quantity of the OaAn-1 protein coding gene in rice, thereby shortening the miscanthus length of the rice.

The reduction or inhibition of the expression level of the gene encoding the OaAn-1 protein in rice can be achieved by any method known in the art, such as chemical mutagenesis, physical mutagenesis, RNAi, site-directed genome editing, or homologous recombination, to cause deletion, insertion, or base transition mutation in the gene, thereby achieving reduction or loss of the gene function. In any of these methods, the entire gene encoding the OaAn-1 protein may be targeted, and each element regulating the expression of the OaAn-1 gene may be targeted, as long as the loss or reduction of the gene function can be achieved. For example, the exon of the gene encoding OaAn-1 can be targeted.

In the above-mentioned genome site-directed editing method, Zinc Finger Nuclease (ZFN) technology, Transcription activator-like effector nuclease (TALEN) technology, Clustered regularly spaced short palindromic repeats and their related systems (Clustered regularly interspaced short palindromic repeats/CRISPR associated, CRISPR/Cas9 system) technology, and other technologies capable of realizing genome site-directed editing can be used.

Alternatively, the method comprises introducing into the rice a substance that reduces or inhibits the expression of the gene encoding the OaAn-1 protein according to the above method.

For example, a nuclease (i.e., a substance which reduces or inhibits the expression of the gene encoding the OaAn-1 protein) specific to a target fragment is introduced into the rice, the target fragment is sheared under the action of the nuclease, and then the target fragment is subjected to site-directed modification through the self-DNA repair of the rice, so that the function reduction or the loss of the gene encoding the OaAn-1 protein is realized.

Among the above methods, the method for expressing a nuclease specific to a target fragment in rice may be: directly introducing a nuclease or genetic material for expressing the nuclease into rice. Wherein the genetic material is a DNA plasmid or a linear fragment of DNA or in vitro transcribed RNA. Nuclease refers to an active functional substance that undergoes site-directed editing.

The method for expressing the nuclease specific to the target fragment in the rice can further comprise the following steps: and planting and growing the rice after the nuclease or the genetic material is introduced under the condition of hygromycin selective pressure.

Alternatively, when the CRISPR/Cas9 method is used, the genetic material is a recombinant vector or linear fragment of DNA capable of transcribing a guide RNA and expressing Cas9 protein; or the genetic material consists of a recombinant vector or linear fragment of DNA capable of transcribing a guide RNA (or two recombinant vectors or linear fragments of DNA capable of transcribing crRNA and tracrRNA, respectively), and a recombinant vector or linear fragment of DNA or RNA capable of expressing a Cas9 protein; or the genetic material consists of a guide RNA, and a recombinant vector or linear fragment of DNA or RNA capable of expressing Cas9 protein; the guide RNA is RNA with a palindromic structure formed by combining crRNA and tracrRNA through partial base pairing; the crRNA contains an RNA fragment that is capable of complementary binding to the target fragment. In the recombinant vector, the promoter that initiates transcription of the nucleotide sequence encoding the guide RNA may be the OsU3 promoter or the OsU6a promoter.

Alternatively, when a TALEN nuclease is used, the genetic material for expressing the nuclease specific for the target fragment can be a recombinant plasmid that simultaneously expresses paired TALEN proteins, or a linear fragment of DNA that simultaneously expresses paired TALEN proteins; or RNA that simultaneously expresses paired TALEN proteins; the TALEN protein consists of a DNA binding domain and a Fok I domain capable of recognizing and binding to the target fragment;

alternatively, when zinc finger nucleases are used, the genetic material for expressing nucleases specific for the target fragment may be a recombinant plasmid simultaneously expressing paired ZFN proteins, or linear fragments of DNA simultaneously expressing paired ZFN proteins; or RNA simultaneously expressing paired ZFN proteins; the ZFN protein consists of a DNA binding domain and a fokl domain that is capable of recognizing and binding to the target fragment.

Optionally, according to the above method, the substance reducing or inhibiting the expression of the gene encoding the OaAn-1 protein is any one of the above biomaterials D1) -D4).

Optionally, according to the above method, the nucleic acid molecule of D1) is a DNA molecule that expresses a gRNA targeting the OaAn-1 protein-encoding gene or a gRNA targeting the OaAn-1 protein-encoding gene; the target sequence of gRNA of the OaAn-1 protein coding gene is shown as SEQ ID No.7 and/or SEQ ID No. 8. The OaAn-1 protein encoding gene can be subjected to site-directed editing by a CRISPR/Cas9 method, so that the expression level of the OaAn-1 protein encoding gene in the plant is reduced or inhibited, for example, a recombinant plasmid vector expressing Cas9 protein and gRNA is infected by agrobacterium to realize the transfer and integration of a foreign gene to a plant cell, and specifically, the recombinant plasmid CRISPR-OaAn-1-2Target prepared in the following examples can be used.

The CRISPR-Oaan-1-2Target is prepared by the following method: the designed Target primers are respectively constructed into intermediate vectors pYLsgRNA-OsU3 and pYLsgRNA-OsU6a by a fusion PCR method, and then the fragments are connected with a pYLCISPR/Cas 9Pubi-H vector skeleton by a 'gold gate cloning' method to obtain a recombinant vector which is recorded as CRISPR-OaAn-1-2 Target.

Optionally, according to the above method, the method for reducing the content and/or activity of oan-1 protein in plants and/or reducing or inhibiting the expression level of the oan-1 protein encoding gene in plants comprises subjecting the oan-1 protein encoding gene represented by SEQ ID No.5 or SEQ ID No.6 in rice genome to at least one of the following mutations:

1) inserting a nucleotide T between 17 th and 18 th nucleotides in a target point SEQ ID No.8 of the Oaan-1 protein coding gene shown in SEQ ID No.5 in a rice genome, or deleting a G;

2) a nucleotide T is inserted between the 17 th to 18 th nucleotides in the target point SEQ ID No.7 of the Oaan-1 protein coding gene shown in the SEQ ID No.6 in the rice genome or a nucleotide A is inserted and/or a nucleotide T is inserted between the 17 th to 18 th nucleotides in the target point SEQ ID No.8 or a G is deleted.

The above method is applicable to any rice plant, only containing the above target sequence, and examples of the present invention are long-stalked wild rice (Oryza alta, CCDD, 2 n-4 x-48).

Because the high-stalk wild rice is a tetraploid plant, the rice obtained by the method after transformation can be rice with all dyeing monomers edited at fixed points, and can also be rice with part of the dyeing monomers edited at fixed points, 2target points are designed by the method, and any one target point plays a role, so that the awn length can be shortened.

The OaAn-1 protein, the OaAn-1 protein coding gene and the biological materials related to the OaAn-1 protein are also in the protection scope of the invention.

According to the specific embodiment of the invention, the OaAn-1 protein coding genes OaAn-1-CC and OaAn-1-DD are simultaneously knocked out by a genome editing technology, so that the rice with obviously shortened miscanthus length is obtained.

Drawings

FIG. 1 is a design drawing of Oaan-1 target in O.alta

FIG. 2 is a diagram of the partial backbone and double-target structure of pYLCRISPR/Cas9Pubi-H vector.

FIG. 3 is the electrophoresis diagram of the second round PCR amplification expression cassette in the plasmid CRISPR-Oaan-1-2Target constructed in example 1.

FIG. 4 is a PCR electrophoresis chart of bacterial liquid constructed by the plasmid CRISPR-OaaN-1-2Target in example 1, and a band at an arrow is a Target band.

FIG. 5 shows the results of the amplification of OaAn-1-CC and OaAn-1-DD from the T0 transformed shoots of example 1, wherein lanes 1-12 show the results of the amplification of T0 transformed shoots, respectively.

FIG. 6 shows the sequencing results of the partially positive seedlings in example 1.

FIG. 7 shows the comparison of awns of example 2 partially positive-edited seedlings and wild type.

FIG. 8 is a statistical analysis of the data for the partially positive edited shoots and wild type length (Awn length) of example 2, averaged. + -. standard deviation, and for significance, P < 0.01.

Detailed Description

The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The examples provided below serve as a guide for further modifications by a person skilled in the art and do not constitute a limitation of the invention in any way.

The experimental procedures in the following examples, unless otherwise indicated, are conventional and are carried out according to the techniques or conditions described in the literature in the field or according to the instructions of the products. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Expression vectors pYLsgRNA-OsU3, pYLsgRNA-OsU6a, binary vector pYLCRISPR/Cas9Pubi-H, CRISPR knockout vector construction methods are disclosed in the literature "Ma, X., et al. A robust CRISPR/Cas9 system for compatibility.high-efficiency multiplex gene editing in monocot and di cot plants.molecular Plant 8, 1274-1284. (2015)", and publicly available from the institute of genetics and developmental biology.

The tetraploid wild rice in the following examples is wild rice Oryza alta 2007-24, which is a high-stalk wild rice seed material (o. alta Swallen, chromosome set CCDD, No. YD-7900) in left column 1.1 of page 906 of document 1, and publicly available from the institute of genetics and developmental biology or the national germplasm nanning wild rice nursery. Document 1: douglas et al, research on callus-induced differentiation of high-stalk wild rice, journal of agriculture in the southwest, 2014, 27(3), 905 and 909.

Tetraploid wild rice Oryza alta is an allotetraploid, with most of the genes having copies on both the CC and DD subgenomes. Through comparison analysis, two homologous genes of An-1 exist in Oryza alta, the gene positioned on the chromosome 4 of the CC subgenome is named as Oaan-1-CC, and the other gene positioned on the chromosome 4 of the DD subgenome is named as Oaan-1-DD. The sequence of OaAn-1-CC is shown in SEQ ID No.5, the amino acid sequence of the coded OaAn-1 protein is shown in SEQ ID No.1, and the CDS of the OaAn-1 protein is shown in SEQ ID No. 3. The sequence of OaAn-1-DD is shown as SEQ ID No.6, the amino acid sequence of the coded OaAn-1 protein is shown as SEQ ID No.2, and the CDS of the OaAn-1 protein is shown as SEQ ID No. 4.

Example 1 knocking out OaAn-1 protein encoding Gene in Rice to shorten Rice growth

1. Construction of positive editing seedlings by knocking out OaAn-1 protein coding genes in rice

The plasmid CRISPR-Oaan-1-2Target is transformed into agrobacterium EHA105 by an electric excitation method, recombinant agrobacterium is utilized to infect callus of tetraploid wild rice, and then resistance screening, differentiation regeneration and rooting culture are sequentially carried out to obtain T0 generation transformed seedlings.

Specific primers of the OaAn-1 on the CC and DD subgenomic group are designed, the obtained T0 generation transformed seedlings are amplified by the specific primers of the OaAn-1-CC and the OaAn-1-DD (as shown in figure 5), then sequencing is carried out to determine the mutation type, and the T0 generation transformed seedlings containing the mutation are named as positive editing seedlings. FIG. 6 shows mutation types of positive editing seedlings T0-1 and T0-2, specifically T0-1, wherein OaAn-1-CC has a T inserted homozygously at target point 2, and OaAn-1-DD frame shift mutation (a A inserted homozygously at target point 1, and a T inserted homozygously at target point 2). In T0-2, OaAn-1-CC has biallelic mutation (one chromosome is inserted with one T, one chromosome is deleted with one G), and OaAn-1-DD frame shift mutation (one T is homozygously inserted at target point 1, one G is homozygously deleted at target point 2), so that the function of OaAn-1 protein is lost.

The primer pair for amplifying OaAn-1-CC is as follows

OaAn-1-CC-F：5’-CGGTACCATACTCCGACCAC-3’

OaAn-1-CC-R：5’-TGGCACGAGCGACTGCAGCA-3’

The primer pair for amplifying OaAn-1-DD is as follows

OaAn-1-DD-F：5’-AACAAACACTGCTCCGGCCAC-3’

OaAn-1-DD-R：5’-TGGCACGAGCGAGTGGAGAA-3’

The plasmid CRISPR-Oaan-1-2Target was prepared by the following method.

Design of first, target sequence

Designing targets in conserved regions of the first exons of OaAn-1-CC and OaAn-1-DD.

The target sequences (i.e., targets) are as follows:

target site 1: 5'-CCGAAGCGGTCCAAGGTCGC-3' (PAM is CGG, SEQ ID No.7, corresponding to position 996-1015 of SEQ ID No. 6)

Target site 2: 5'-ACATCCATGTGAGGGCGAGG-3' (PAM is AGG, SEQ ID No.8, corresponding to positions 1016-1035 of SEQ ID No.5 and 1087-1106 of SEQ ID No. 6)

Design of second, gRNA

gRNA was designed based on the target sequence.

Construction of recombinant plasmid

The following single-stranded primers with linker sequences (underlined) were synthesized:

U3-OaAn-1-F：5’-CCGAAGCGGTCCAAGGTCGCGTTTTAGAGCTAGAAAT-3’

U3-OaAn-1-R：5’-GCGACCTTGGACCGCTTCGGTGCCACGGATCATCTGC-3’

U6a-OaAn-1-F：5’-ACATCCATGTGAGGGCGAGGGTTTTAGAGCTAGAAAT-3’

U6a-OaAn-1-R：5’-CCTCGCCCTCACATGGATGTCGGCAGCCAAGCCAGCA-3’

synthesizing single-stranded primers for constructing the vector:

U-F：5’-CTCCGTTTTACCTGTGGAATCG-3’

gRNA-R：5’-CGGAGGAAAATTCCATCCAC-3’

U-F, gRNA-R, U3-OaAn-1-F, U3-OaAn-1-R is used as a primer, pYLsgRNA-OsU3 plasmid is used as a template to perform fusion PCR to amplify the pOsU3-OaAn-1gRNA expression cassette. U-F, gRNA-R, U6a-OaAn-1-F, U6a-OaAn-1-R is used as a primer, pYLsgRNA-OsU6a plasmid is used as a template to perform fusion PCR to amplify the pOsU6a-OaAn-1gRNA expression cassette. The PCR product is diluted 10 times and used as a second round PCR amplification template.

Synthesis of second round PCR amplification primers:

B-L：5’-TTCAGAGGTCTCTCTCGACTAGTATGGAATCGGCAGCAAAGG-3’

B2：5’-AGCGTGGGTCTCGTCAGGGTCCATCCACTCCAAGCTC-3’

B2’：5’-TTCAGAGGTCTCTCTGACACTGGAATCGGCAGCAAAGG-3’

B-R：5’-AGCGTGGGTCTCGACCGACGCGTATCCATCCACTCCAAGCTC-3’

B-L, B2 was used as a primer and pOsU3-OaAn-1gRNA expression cassette was used as a template for the second round of PCR amplification. B2' and B-R are used as primers, and pOs6a-OaAn-1gRNA expression cassette is used as a template for carrying out second round PCR amplification. The amplified products were detected by agarose gel electrophoresis, and the fragments were recovered as 564bp and 629bp bands, respectively, as shown in FIG. 2. And (5) purifying and recovering. The second round of purified product was constructed into pYLCRISPR/Cas9Pubi-H (restriction enzyme BsaI, ligase T4 ligase) according to the routine molecular experimental procedure "edge-cut ligation". After transformation of DH 5. alpha. a single clone was picked up and subjected to PCR, a positive single clone with a band size of about 1.2kb was selected and sequenced (FIG. 3). The sequencing primer is SP1 (5'-CCGACATAGATGCAATAACTTC-3'), and the recombinant plasmid with correct sequencing is named as CRISPR-OaaN-1-2 Target.

A schematic diagram of a partial vector backbone of pYLRISPR/Cas 9Pubi-H is shown in FIG. 1A, wherein a Pubi promoter, a nuclear localization signal sequence (NLS), a Cas9 encoding gene (Cas9), a nuclear localization signal sequence (NLS) and a Tnos terminator are connected in sequence. The schematic structural diagram of the double-Target sequence in the CRISPR-oan-1-2 Target is shown in fig. 1B, wherein OsU3 promoter, Target 1 sequence (expressing gRNA targeting Target site 1), OsU6a promoter and Target 2 sequence (expressing gRNA targeting Target site 2) are connected in sequence. In the plasmid CRISPR-Oaan-1-2Target, the aforementioned double Target sequence is ligated after the Tnos terminator.

2. Phenotypic identification of positively-edited shoots

The positive seedlings prepared above, T0-1, T0-2 and tetraploid wild rice (o.alta, wild type), were planted under natural conditions, the field phenotype was observed and the awn length was measured.

As shown in FIGS. 7 and 8, when the OaAn-1-CC and OaAn-1-DD genes are subjected to frame shift mutation, the An-1 protein function is lost, and the awn length is obviously shortened at the T0 generation. The length of wild-type awns is 4.11cm (average value of 20 awns), the length of T0-1 awns is 1.61cm (average value of 20 awns), the length of T0-2 awns is 2.63cm (average value of 20 awns), the length of the seedling awns is shortened in positive editing, and harvesting is facilitated. The invention proves that the rapid domestication of the miscanthus sinensis length can be realized by editing the major gene Oaan-1 of the miscanthus sinensis length in tetraploid wild rice, the miscanthus sinensis length is shortened, and the harvest and the processing are convenient.

The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.

Sequence listing

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gaccagatga tgatgatggt gccagcggcg gcggagtccg tcgcgcactt cgactccgcg 180

ctcagctcgc tcgtgtcgtc gccgggtgga ggtggcgggc ttgtgaatgg caatggtgga 240

gggggtggtg atgagatggc ggccatcggc gacctgatcg ggcggctggg gagcatctgc 300

aacaacgggg ccagcgccaa caactcctgc tacagcacgc cactcagctc cccgccgcgc 360

ggcggcccgc ctccggtgac ggcgttccgt ggctacgccg ccgtggacac cggcaggctg 420

tccagggtct ccagcagcaa gtccctcggc gccggcgccg ccgccaccgc cgccgcattg 480

gacagctccg aggcggagat gagccccacc accgccgccg gtgaccaacc agccaagccc 540

tccgcctccg ccgccgccgc cgcccgcaag cgcaagtcct cggcgaagcc caaggcctcc 600

tcctcatcct tacccacggc tacggcagcg acgacgaacg cgagcccgaa gcggtccaag 660

gtcgtcgccg ccgccgccgg cggcggacac gacggcgacg gcgacgcggc gacgcaggag 720

gagaaacccg agccgaccaa agactacatc catgtgaggg cgaggagggg acaagccacc 780

gatagccata gcctcgccga gagggtgagg agggagagga taagcgagag gatgaagctg 840

ctgcagtcgc tcgtgccagg ctgcaacaag atcaccggca aggctctcat gctggacgag 900

atcatcaact atgtccagtc gctgcagcgt caggtcgagt ttttgtccat gaagttagcg 960

accatgaatc ctcagctgga ctttgacggc cattacatgc cttccaaaga tatgaaccac 1020

atgccagtac ccacataccc gtcaggcgac ccaaccaccg ccaccgtgtt ctcctacacc 1080

ggctctcctg ctactgccga tccattcaac ccctataact gttgggagct cgaccttcat 1140

accgcaatgc aaatgagagc cgccgccgcc accgggctca gccaagatgg cctagtggca 1200

gcgatggcat cctcgccatt gccacaccaa cctcctcacg gcttctatgg ggacgacatg 1260

aaggccgagc cgtaa 1275

<210> 4

<211> 1335

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 4

atggccgacg agcacttctt ccccgccgga gattacttct cctcctcctc cggcgccacg 60

gggacgggcg cgttactgcc cggcgggctg gacgtcgccg ccgcggcggc atacgggatg 120

atgccgccct gggcagtggc cgaccagatg atgatgatga tggtgccggc ggcgggggcg 180

gaggcggagt gcgttgcgca cttcgactcc gcgctcagct cgctcgtgtc gtctccgggt 240

ggaggtggcg ggcttgtgaa cggcaatggc ggcggctgcg gcgggggtga tgagatggcg 300

gccatcggcg acctgatcgg ccggctcggg agcatctgca acggcgccag cgccaacaac 360

tcctgctaca gcacgccgct cagctccccg ccgcgcggcg gcccgccgcc acctccgccg 420

ctgacggcgt tccgtggcta cgccgccgtg gacaccggca ggctgtcccg ggtctccagc 480

agcaagtccc tcggcgccgc cgcattggag agctccgagg cggagatgag ccccaccacg 540

gccgccggcg accaaccagc caagccctcc gccgccgcag ccgccgccgc ccgcaagcgc 600

aagtcctcag cgaaggccaa ggccacctcc tcctccttac ccacggctac ggcagcgacg 660

aatgcgagtc cgaagcggtc caaggtcgcc ggcggcggcg gtggcgacgg cgacggcgac 720

gcggcggcgg aggaggagaa gcccgagccg gccaaagact acatccatgt gagggcgagg 780

aggggacaag ccaccgatag ccacagcctc gccgagaggg tgaggaggga aaggataagc 840

gagaggatga agcttctgca gtcgctcgtg ccaggctgca acaagatcac cggcaaggct 900

ctaatgctgg acgagatcat caactatgtc cagtcgctgc agcgtcaggt cgagtttttg 960

tccatgaagt tggcgaccat gaatcctcag ctggactttg acggccatta tataacttcc 1020

aaagatacga gtcatatgcc aatgctcgca tacccgtcag gcgacctgac caccgccacc 1080

gcgttctcct ataccggctc acccgccgcc accgccgccg atccattcgc cgcctacaac 1140

tgctgggagc tcgtcgacct ccacaccaag atgcaaatag gagccaccac cactggcctc 1200

aaccaagatg gcccatcggt cgcagcgatg gcaccctcgc cttcaccacc attgccgcac 1260

catcctcctc acggcttcta cggggggcag aagcagcagg ggacgacagt aaaccacatg 1320

aaggccgagc cgtaa 1335

<210> 5

<211> 5064

<212> DNA

<213> wild rice (Oryza alta)

<400> 5

gatagtggca gtatatcttt tcttccgcgt tattaccgtg ctcgtgccgg catctcatct 60

cttcccaccc accaacacgg ttacttccct ccccttccca accatttctt atatataacg 120

gtcaaccacc gcctccctct ttccctctcc tctctcctct tcctccactc ccacccactc 180

tcccacgcgc cacgccgcca ccgatggccg acgagcactt cttccccgcc ggagattact 240

tctcctccgg cgccgccacg gggacgggcg cgttgctgcc cgccgcgtcg tacgggatga 300

tgccgccgtg ggcggtggcc gatgaccaga tgatgatgat ggtgccagcg gcggcggagt 360

ccgtcgcgca cttcgactcc gcgctcagct cgctcgtgtc gtcgccgggt ggaggtggcg 420

ggcttgtgaa tggcaatggt ggagggggtg gtgatgagat ggcggccatc ggcgacctga 480

tcgggcggct ggggagcatc tgcaacaacg gggccagcgc caacaactcc tgctacagca 540

cgccactcag ctccccgccg cgcggcggcc cgcctccggt gacggcgttc cgtggctacg 600

ccgccgtgga caccggcagg ctgtccaggg tctccagcag caagtccctc ggcgccggcg 660

ccgccgccac cgccgccgca ttggacagct ccgaggcgga gatgagcccc accaccgccg 720

ccggtgacca accagccaag ccctccgcct ccgccgccgc cgccgcccgc aagcgcaagt 780

cctcggcgaa gcccaaggcc tcctcctcat ccttacccac ggtaccatac tccgaccacc 840

gctccatcac ttctccgatc cccagctgac caacccataa taatgcaggc tacggcagcg 900

acgacgaacg cgagcccgaa gcggtccaag gtcgtcgccg ccgccgccgg cggcggacac 960

gacggcgacg gcgacgcggc gacgcaggag gagaaacccg agccgaccaa agactacatc 1020

catgtgaggg cgaggagggg acaagccacc gatagccata gcctcgccga gagggtaatt 1080

acctaatcaa ctaattaacc atcctctcct aattaaatcc taattagctt tttgcaagta 1140

cactaatccc caaattaatc acaatgcgcg gcacccccta attgcgcacc gttttactcc 1200

tacttgtcac taattgctct acttaattag taagttgcaa ggacgcttaa ttattaaaat 1260

ttaattagct tgtgttttgt ttaatggtag gtgaggaggg agaggataag cgagaggatg 1320

aagctgctgc agtcgctcgt gccaggctgc aacaaggtat gaacacagca ctctccagct 1380

gcaatctcat gcttaaattt tatccaaatt tatctatcca ttactccaat tttaaaattc 1440

tagccatctc tccatccatg ttactagcta gtttagagtt taatatcaaa atgtgcgaaa 1500

attttgtctc gaaaaatagt accaatttgt aatatcagaa atccgattta ttttacaatt 1560

acatagcggt actagaaatt agtggtcaaa aatgtattta agagaacgca cattaagggg 1620

gaatagtaac ctaacgtaca aaacggtctt acctgtgtgg acggagagaa cagtaattaa 1680

gtttaatata tatatataat tactttagtt tcagtattgc ctagagctag gagttggact 1740

ttttgctagt aggatagata aaattaagtg caattaatca attattttgt accgaaagta 1800

tgagcactaa ctgcggtggt tagtctacgc agccagtagt aattttgacc gtaatgcatt 1860

gtaagctacc agtagtactc ccaagttaag ctcccagtgg tccaagaaga tataataata 1920

attgattaat taataatctg agaggctctt gctctgtact taaactaatc aaaccggcta 1980

atcaattgca aggtcctggt ggtttaggtg gtgcgcacga ttattttagt tgccccaatt 2040

attgccacgg tagtgatgcc cctgccggtg gtcaaatttg ggttgaattt taaagttggt 2100

tgataacttt tatacctttt taaaaattat tgatcagttt ttttgctgga tgatcgttta 2160

ttgtctttta gcaaataaat atgaatgttg cggtgtgcag atcaccggca aggctctcat 2220

gctggacgag atcatcaact atgtccagtc gctgcagcgt caggtcgagg taccaatgca 2280

acatttgctt tagtagtgtg ctaaatgaac ttgttttaat gacaataaat ttgttggaga 2340

aaaatgttgt tgtgttctcc ttctgtccta gttagctctt aattgaagtt ctgtatgaaa 2400

ttaaacccaa taatagctaa attttggcat tggtgtgtct cacagttttt gtccatgaag 2460

ttagcgacca tgaatcctca gctggacttt gacggccatt acatgccttc caaagatgta 2520

agtagcatct ttcaatttgt tactaaaaga aaacaatgct atgtgatctg gatatcaaac 2580

ggttaagata gagtattatt cgatattgtc cctccatttg caaatgttat aacgaccgca 2640

ctcaccatat cattttcaga tgaaccacat gccagtaccc acatacccgt caggcgaccc 2700

aaccaccgcc accgtgttct cctacaccgg ctctcctgct actgccgatc cattcaaccc 2760

ctataactgt tgggagctcg accttcatac cgcaatgcaa atgagagccg ccgccgccac 2820

cgggctcagc caagatggcc tagtggcagc gatggcatcc tcgccattgc cacaccaacc 2880

tcctcacggc ttctatggca agtgaaacca aaccatcacc tctcctcgta accctaacaa 2940

atcatacata gacttttaaa atactcaaaa aaaaatcaaa acataagttt tttttttact 3000

agactacaat actggtcata ctactccagt agtactggta cggtaggttt gtttgtccaa 3060

gtttgttgag tttcgctgtt ggtggtaatt gggtgcaggg gacgacatga aggccgagcc 3120

gtaataaatg ggcgacctcc ttggccttcc cttctgtaca tacgccagcc gccgtacgtg 3180

tactccgctt ttctgctccc atcctgcagc atcagcatca gcagcagcag cagacccagc 3240

tcacaatcat tgccatcaaa gagaaagaag aggatttgtg tgtaatgctt catggcttga 3300

tgatggcttt ggctccctag cttcccttgc tcttcttctc actggatatg tgggccctct 3360

catatgtggc agcatcttct tttcaccttt ctcccctctg ccctctgcca ctgcaggtgg 3420

ggcccacctg catagggaag gagaaggttt ttcagatgca ttggtggcat gatgaagggg 3480

cacattgtca ttgtttatgc aagaacttgt ttgtgttgtg atgttgtagg acaagaggag 3540

tatgtgtgtg tttggaaatg gcaaggcagg ggagttgtgt gaccttttgt gtgtgctggt 3600

tgcatgccct aactgtagaa aaaaatggac gactgtggta gtgatgtagt ggtagtggct 3660

ttgtaggatt ggcatgcatg gggatgtatg tacttctgta tgtagagatt ggagagggtt 3720

ttgaagaaca ccttcctttt tggtgaacat tgtttgtatc aaaagtactg gaatcataat 3780

gcatgcaaga aatgtttttg aacaatattc gacaatgtcg ttagtatttt tgatgtggtt 3840

gtctatatta gtttagaatc ctttggtttg tcaaaagtta tccgcattac aatctggaag 3900

gatttaggat cttggggaca atgagaggat gagcgacaat attactatga ttatatatca 3960

cattcgcttc atctttcacc agtttttgca atagattcat gtgttgatcc ttgtcactag 4020

agcaattttt ttatcaatga actaaagata tatacccaat tgagccacaa agatgccatg 4080

gcctccatct gcattttgtt gtgacttctt aagaaattga tccatcatca tctgcatgtt 4140

gctatgactt ctggagaaat tgatccatca ttgatgcaga gtctgcatac gtactatgag 4200

gagtatgcaa taacttttag cttcttccaa atgatcggtc ggcaaaaaaa aacatgtgtt 4260

tcttgtgtca ctaataccat aattcatagg catgtcgacg attgtgctct tcctataacc 4320

atagctaacc cccctacaac caacacacaa ttaaagtgtt gaaccaacaa aatggtacta 4380

catcaataat gcatcaacct atgaaatgaa aagataactt cttcctttta ccagcattgg 4440

catttccaac caagatctcc ctagatgcac acaacatgat cagtgttaat catgggattc 4500

tctctaatca cctccacact ctcaaatcat tgtttcatct gttgccatat cctaatcaca 4560

gaaggcccca cagtgctttc tctctgtgga atctagccag tagggggagg gggcttgatc 4620

ccagctggca tgtgggcccc cacccacatc aacccttttg gatcttcttc ttcttcttct 4680

attctactcc tacatctctc tttcacattg cagctactag ctgacattgg agtacttgac 4740

aagtccttaa acaagtgcac ttttggggcc tttaattcag ttgtaatagt agtagtagaa 4800

gtaattaaat ttcttgttgt cttgtgtagc attttcttct tcatgtcttt gcccttcatt 4860

aataagtcaa gtgatgtgac aatggatgct ggaccctttt ttacctgcct ccctgaccgg 4920

ccttccttta tgcagacatc aagtcggtga gtaccaaagc agctcaactg taattctgtc 4980

attaagttgg tgattagtgc atgcaaattg tcattagcat cagtacagtt aggtgatgat 5040

taaattaaag tacagttgtg tgag 5064

<210> 6

<211> 5367

<212> DNA

<213> wild rice (Oryza alta)

<400> 6

ctataaattt actttaaaat atcacatctt aggtctactt agattgaaag gagagagtat 60

ttggttccgc gttattacgg ccggcatctc ttcccagttc ccacccacca atacggttac 120

ttccctcccc tttcccaaac gatttcttat ataacggtca accaccgccc ctctcttttc 180

cctctccgct ctcctcctcc actcccaccc actctcccac gcgccacgcc gccaccgccg 240

acgacgacga tggccgacga gcacttcttc cccgccggag attacttctc ctcctcctcc 300

ggcgccacgg ggacgggcgc gttactgccc ggcgggctgg acgtcgccgc cgcggcggca 360

tacgggatga tgccgccctg ggcagtggcc gaccagatga tgatgatgat ggtgccggcg 420

gcgggggcgg aggcggagtg cgttgcgcac ttcgactccg cgctcagctc gctcgtgtcg 480

tctccgggtg gaggtggcgg gcttgtgaac ggcaatggcg gcggctgcgg cgggggtgat 540

gagatggcgg ccatcggcga cctgatcggc cggctcggga gcatctgcaa cggcgccagc 600

gccaacaact cctgctacag cacgccgctc agctccccgc cgcgcggcgg cccgccgcca 660

cctccgccgc tgacggcgtt ccgtggctac gccgccgtgg acaccggcag gctgtcccgg 720

gtctccagca gcaagtccct cggcgccgcc gcattggaga gctccgaggc ggagatgagc 780

cccaccacgg ccgccggcga ccaaccagcc aagccctccg ccgccgcagc cgccgccgcc 840

cgcaagcgca agtcctcagc gaaggccaag gccacctcct cctccttacc cacggtacca 900

caacaaacac tgctccggcc accgccgctc catcacttct ccgatcccca gctgaccaac 960

ccataatgca ggctacggca gcgacgaatg cgagtccgaa gcggtccaag gtcgccggcg 1020

gcggcggtgg cgacggcgac ggcgacgcgg cggcggagga ggagaagccc gagccggcca 1080

aagactacat ccatgtgagg gcgaggaggg gacaagccac cgatagccac agcctcgccg 1140

agagggtact actacctaat taaccatcca ttatccccta attatctttt gcaagtacac 1200

taatccccaa attaatcaca atccgcggcc cccctaattg cgcaccgtgt tactacttgt 1260

cactaattgc tctacttaat taagttgcaa ggacacttaa ttattgttta acggtaggtg 1320

aggagggaaa ggataagcga gaggatgaag cttctgcagt cgctcgtgcc aggctgcaac 1380

aaggtatgaa cacaacactc tccagctgca atctctcatg cttaaatttc cttcaaattt 1440

atctatccat tactccaatt ttaaaattct agcaatcctc ctccatccat gttactagtt 1500

tagagtctaa tatcgaaatg tgcgaaaaag aattcctgaa aagtagtacc aatttgtaat 1560

attagaaacc cgctatattt tacgattata agcggtacta taaattagtc atcaaaatag 1620

tatttcggtg aacgcgcatt aagggagaat agtaacctaa catacaaaat ggacttacct 1680

gtgtggacgg agagaacaat aattaagttt aacatatata attactttag tttcagtatt 1740

gcctagagct aggagttgga ctttttgcta ctaggataga taaaattaag tacaaataat 1800

caattttttg taccggatgt atgagcacta actgcggtgg ttagtctaca cagccagtag 1860

taattttgac cgtaatgcat ctcctaggtt aagctcccaa tggtccaaga agatataata 1920

ataattgatt aattaataat ctgataggct cttgctctgc acttaaacta atcaaaccgg 1980

ctaatcaatt gcaaggtcct ggtggttttg gtggtgcgca cgattatttt agttgcccca 2040

attattgcca cggtagtgtg ccccagccgg tggtcaaatt tgggttgaat tttaaatttg 2100

gttgataact tttctacctt tttaaaaatt attgttgagt tttttttgtt ggatgattgt 2160

tcattgtttt tgtttagtca atgtgaatgt tgcggtgtgc agatcaccgg caaggctcta 2220

atgctggacg agatcatcaa ctatgtccag tcgctgcagc gtcaggtcga ggtaccaatg 2280

caacattgct ctaatagtgt gctaaatgaa cttgttttaa tgacaataat tcgttggaga 2340

aaattgttgt tgtgtgctcc ttctgtccta gactagctct taattgaagt tttgtatgga 2400

accaaatagc taaattttaa cattttgaca ttggtgtgtc tcatagtttt tgtccatgaa 2460

gttggcgacc atgaatcctc agctggactt tgacggccat tatataactt ccaaagatgt 2520

aagtagtatc ttcaatttgt tacgtactaa aagagaaaac atttgatatc tggacacaaa 2580

caaacattgc cactatgttt ttccctcaat ttgcaaatgt tataacgacc gtaattaccc 2640

ctatcatttt cagacgagtc atatgccaat gctcgcatac ccgtcaggcg acctgaccac 2700

cgccaccgcg ttctcctata ccggctcacc cgccgccacc gccgccgatc cattcgccgc 2760

ctacaactgc tgggagctcg tcgacctcca caccaagatg caaataggag ccaccaccac 2820

tggcctcaac caagatggcc catcggtcgc agcgatggca ccctcgcctt caccaccatt 2880

gccgcaccat cctcctcacg gcttctacgg caagtgaaac caaaccacca gatcttctca 2940

tatccttaaa aaaagattaa aacatacatt ctctatttca aagtattgct acctagtaca 3000

tgattggaca ctttctagta caacgaatct aaatagagaa cctatctaga tgcgtctgta 3060

tcgctccctt catcccaaat tatagtatac ccttattttt agaaaaatca aactcggtaa 3120

cttttgacta ataatcattc taacaatatc tataagaagt attatgcgtg ctatatcact 3180

agatttatat ttcaaattag ttttatatga tactaatttt atatctattg gtaacgtaac 3240

attaaataaa ttaatggtca aaatatgtca ttgaagaccg tgtaaaaaat ttataagact 3300

tataattaaa caaagtaagt aagaaatgtt caatgttaac attttaggac agagatagta 3360

cgctgttttt tctctgtggt actggaccat agtagtggtc atggtgtagg tttgtttgtc 3420

caagtttgtt gagtttcgct gttggtggta attgggcgca ggggggcaga agcagcaggg 3480

gacgacagta aaccacatga aggccgagcc gtaataaatg cgcgacctcc ttcgccttcc 3540

ttctgtacat acgccagccg ccgtacgtac tccgcttttc tgctcccatc ctgcagcatc 3600

agcatcagca gcagcaaacc cagctcacaa tcattgccat caaagagaag aagaggatgt 3660

gtgcttggct ccctcctcct cctccctctc actggatatg tgggccctct catatgtggc 3720

agcatctttt tccccccctt tctccccctc tgccctctgt cactgcaggt ggggcccgtc 3780

tgcataggga aggagaaggt ttttcaggct caactttttc agatgcattg gcatgcaggc 3840

aacattgtca ttgtttagca aggactgtag aaaagccagg ggagctgtga ccattgtgtg 3900

tgctggttgc atgccctaac tgtagaaaag gaggactgtg gcttggtagg attggcatgc 3960

atggggatgt atgtactatt atgtatgtag agattggaga gggttttgag gaacaccttc 4020

ctttttgggg ggtgaacatt gtttgtatca gaagtaccga aatcatcgcg catgcaagaa 4080

atgtctttga tcaataaccg acgatgtcgt tagtattttt gatgtggttg tctatgttag 4140

ttcagaattc tttgtttgtc aaaagttatc tgtgttataa tctagacggg tttaggattt 4200

tgggaacaac tagaggatga gcgataatat tattatgatt atatatccta ttcgcttcat 4260

cattcaccag tttttgcaat agattcctgt gttgatcctt gtcattggag caaatttttt 4320

gtcaatgaac taaagatata tacccaattg agccacaacg atgccatggc cttcatctgc 4380

attttgttat gacttcttaa gaaattgatc catcattgat gcagagagtc tgcatatgta 4440

ctatgaggag tatacaataa tttttacctt cttccaaatg atcgatcagc taaaaaagaa 4500

catgtgtttt ctatgtcact aataccatag ttcataggca tgtaggtgat tgtgcgcttc 4560

gtagaaccat agctaatccc cctacaacca acacacagta gaagtgttga accaacaaaa 4620

aatggtacta catgaataat gcatcaacct aatgaaatga aaagataact tcttctcttt 4680

ttttttacca gcattggcat ttccaaccaa gatctcccta gatgcacaca acatgatcag 4740

tgttaatcat gggcttccct ctaatcacct cctcactctc aatcattgtt tcatccgttg 4800

ccatatccta atcacagcag ggccccacag tgctttctct ctgtggaatc tagccagtag 4860

gggggagggg acttgatccc agctggcatg tgggccccac ccacatcaac ccttttggat 4920

cttcttcttc ttctattcta ctcctgcatc tctctttcac attgcagcta ctattagctg 4980

acattggagt acttgacaag tccttgaaca agtgcacttt tggggccttt aattcagttg 5040

tggtggtagt agagataatt aaatttcttg ttgtcttgtg aggcacttac ttgttcatgt 5100

ctttgccctt cattaagtca agtgatgtga caatggatgc tggacccttt tttacctgcc 5160

tccctgaccg gccttccttt atgcagacat caagtcggtg agtaccaagc agctcaactg 5220

taattctgtc attaagttgt tgattagtgc atgcaaattg tcattagcat cagtacagtt 5280

aagagaggat tagattaaag tacagttgtg tgagatgcta actactgatt agagaggtgt 5340

taatttgggg attaaggcaa ggctggc 5367

<210> 7

<211> 20

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 7

ccgaagcggt ccaaggtcgc 20

<210> 8

<211> 20

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 8

acatccatgt gagggcgagg 20

Claims (10)

1. The application is characterized in that: the application of the OaAn-1 protein in regulating and controlling the rice awn length or the application in preparing a product for regulating and controlling the rice awn length,

the OaAn-1 protein is a1) or a2) or a3) protein as follows:

a1) the amino acid sequence is protein shown as SEQ ID No.1 or SEQ ID No.2 in the sequence table;

a2) the protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown in SEQ ID No.1 or SEQ ID No.2 in the sequence table;

a3) protein which has 80% or more than 80% of identity with the amino acid sequence limited by SEQ ID No.1 or SEQ ID No.2 in the sequence table, is derived from rice and has the same biological function.

2. The application is characterized in that: the application of the Oaan-1 coding gene in regulating rice awn length or the application in preparing rice awn length products,

the OaAn-1 protein coding gene is a DNA molecule shown as c1) or c2) or c3) or c 4):

c1) the coding sequence is a DNA molecule shown in SEQ ID No.3 or SEQ ID No.4 in a sequence table;

c2) the nucleotide sequence is a DNA molecule shown as SEQ ID No.5 or SEQ ID No.6 in the sequence table;

c3) a DNA molecule which has 90 percent or more than 90 percent of identity with the nucleotide sequence limited by c1) or c2), is derived from rice and encodes the OaAn-1 protein;

c4) a DNA molecule which is hybridized with the nucleotide sequence limited by c1) or c2) under strict conditions and codes the OaAn-1 protein.

3. The application is characterized in that: the use of a biological material related to the Oaan-1 protein of claim 1 for regulating rice stem length or for preparing a product for regulating rice stem length,

the biological material is any one of the following C1) to C6) and D1) to D7):

C1) an expression cassette containing the OaAn-1 protein coding gene;

C2) a recombinant vector containing the gene encoding the OaAn-1 protein, or a recombinant vector containing the expression cassette of C1);

C3) a recombinant microorganism containing a gene encoding said OaAn-1 protein, or a recombinant microorganism containing C1) said expression cassette, or a recombinant microorganism containing C2) said recombinant vector;

C4) a transgenic plant cell line containing the gene encoding the OaAn-1 protein, or a transgenic plant cell line containing the expression cassette of C1);

C5) transgenic plant tissue containing the gene encoding the OaAn-1 protein, or transgenic plant tissue containing the expression cassette of C1);

C6) a transgenic plant organ containing said gene encoding an OaAn-1 protein, or a transgenic plant organ containing C1) said expression cassette;

D1) a nucleic acid molecule that inhibits or reduces the expression of a gene encoding said OaAn-1 protein;

D2) an expression cassette comprising the nucleic acid molecule of D1);

D3) a recombinant vector containing the nucleic acid molecule of D1) or a recombinant vector containing the expression cassette of D2);

D4) a recombinant microorganism containing D1) the nucleic acid molecule, or a recombinant microorganism containing D2) the expression cassette, or a recombinant microorganism containing D3) the recombinant vector;

D5) a transgenic plant cell line comprising D1) the nucleic acid molecule or a transgenic plant cell line comprising the expression cassette of D2);

D6) transgenic plant tissue comprising the nucleic acid molecule of D1) or transgenic plant tissue comprising the expression cassette of D2);

D7) a transgenic plant organ containing D1) the nucleic acid molecule or a transgenic plant organ containing D2) the expression cassette.

4. A method for shortening the awn length of rice is characterized in that: comprising reducing the content and/or activity of the OaAn-1 protein in the rice as claimed in claim 1 and/or reducing or inhibiting the expression level of the OaAn-1 protein coding gene in the rice as claimed in claim 2, thereby shortening the awn length of the rice.

5. The method of claim 4, wherein: comprising introducing into said rice a substance which reduces or inhibits the expression of said OaAn-1 protein-encoding gene.

6. The method of claim 5, wherein: the substance which reduces or inhibits the expression of the gene encoding the OaAn-1 protein is any one of the biomaterials D1) -D4) of claim 3.

7. The method of claim 6, wherein:

D1) the nucleic acid molecule is a DNA molecule for expressing a gRNA targeting the OaAn-1 protein coding gene or a gRNA targeting the OaAn-1 protein coding gene;

the target sequence of gRNA of the OaAn-1 protein coding gene is shown as SEQ ID No.7 and/or SEQ ID No. 8.

8. The method of claim 1, wherein:

the method for reducing the content and/or the activity of the OaAn-1 protein in the plant and/or reducing or inhibiting the expression level of the OaAn-1 protein coding gene in the plant comprises the step of carrying out at least one mutation on the OaAn-1 protein coding gene shown in SEQ ID No.5 or SEQ ID No.6 in a rice genome, wherein the mutation is carried out on the gene:

1) inserting a nucleotide T between 17 th and 18 th nucleotides in a target point SEQ ID No.8 of the Oaan-1 protein coding gene shown in SEQ ID No.5 in a rice genome, or deleting a G;

2) a nucleotide T is inserted between the 17 th to 18 th nucleotides in the target point SEQ ID No.7 of the Oaan-1 protein coding gene shown in the SEQ ID No.6 in the rice genome or a nucleotide A is inserted and/or a nucleotide T is inserted between the 17 th to 18 th nucleotides in the target point SEQ ID No.8 or a G is deleted.

9. Protein or biological material related to said protein, characterized in that: the protein is the OaAn-1 protein of claim 1.

10. A nucleic acid molecule characterized by: the nucleic acid molecule is the OaAn-1 protein coding gene in claim 2.

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