CN112843086B - Polysaccharide freeze-dried powder with anti-depression effect and preparation method and application thereof - Google Patents
Polysaccharide freeze-dried powder with anti-depression effect and preparation method and application thereof Download PDFInfo
- Publication number
- CN112843086B CN112843086B CN202110138841.9A CN202110138841A CN112843086B CN 112843086 B CN112843086 B CN 112843086B CN 202110138841 A CN202110138841 A CN 202110138841A CN 112843086 B CN112843086 B CN 112843086B
- Authority
- CN
- China
- Prior art keywords
- freeze
- concentrating
- dried powder
- polysaccharide
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/36—Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/24—Antidepressants
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Neurosurgery (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Pain & Pain Management (AREA)
- Psychiatry (AREA)
- Biomedical Technology (AREA)
- Neurology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Botany (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses polysaccharide freeze-dried powder with an anti-depression effect, and a preparation method and application thereof, and belongs to the technical field of medicines. The polysaccharide lyophilized powder is prepared from Lonicera Japonica flos by extracting with water, precipitating with ethanol, collecting precipitate, redissolving to remove protein, concentrating, and lyophilizing. The raw materials are natural and safe, do not cause serious side effect, can effectively play the role of resisting depression, and can be used for preparing the medicine for preventing and treating depression.
Description
Technical Field
The invention relates to the technical field of medicines, in particular to polysaccharide freeze-dried powder with an anti-depression effect and a preparation method and application thereof.
Background
The depression is one of the most common mental diseases, the clinical manifestations of depression are emotional depression, self-mutilation, anhedonia, despair and the like, but some patients are accompanied by anxiety, and severe patients can have suicide attempts or behaviors, and the disease is characterized by repeated attacks, is often accompanied by serious psychological and physiological disorders, and has great harm to human health. With the rapid development of economy in recent years, the pressure of social competition and the like is increasing, and the incidence rate of depression is rapidly increased.
The depression patients often need to take the medicine for a long time, and the treatment period is longer. The current clinical commonly selected antidepressant drugs are selective 5-hydroxytryptamine reuptake inhibitors, monoamine oxidase inhibitors, tricyclic antidepressants, heterocyclic antidepressants and the like; most western medicines have various adverse reactions; there are surveys that about one third of patients who take antidepressant drugs are forced to stop taking drugs due to their intolerable side effects; such as hypertensive crisis, dysphoric symptom aggravation, hyposexuality, and serotonin toxicity (also called serotonin syndrome, which is the main cause of mania, restlessness, insomnia, etc.).
Therefore, the problem to be solved by those skilled in the art is how to excavate a novel antidepressant drug with good curative effect and lower side effect from natural products.
Disclosure of Invention
In view of the above, the invention provides the polysaccharide freeze-dried powder and the preparation method thereof, the polysaccharide is extracted from the raw material of the honeysuckle flower and bupleurum root, the polysaccharide freeze-dried powder is natural and safe, does not cause serious side effects, can effectively exert the anti-depression effect, and can be used for preparing the medicine for preventing and treating depression.
In order to achieve the purpose, the invention adopts the following technical scheme:
a lyophilized polysaccharide powder with antidepressant effect is prepared from herba Melastomatis Crispi by extracting with water, precipitating with ethanol, collecting precipitate, redissolving to remove protein, concentrating, and lyophilizing.
Radix Stellariae (also known as YINHOU, SHANCAGEN, NIUJIGEN, SHASHEN, BAIGEN, etc.) is the dried root of Stellaria dichotoma L (Stellaria dichotoma L) belonging to Stellaria of Caryophyllaceae, is sweet and cold in taste, has effects of clearing deficiency heat and removing infantile malnutrition heat, and is clinically used for symptoms such as fever due to yin deficiency, bone steaming, overstrain heat, infantile malnutrition heat, etc.
The polysaccharide extracted from the raw material of the honeysuckle, the bupleurum root and the Chinese angelica root can effectively exert the anti-depression curative effect, have no toxic or side effect and are safe to take.
A preparation method of polysaccharide freeze-dried powder with an antidepressant effect comprises the following steps:
(1) Taking radix Stellariae decoction pieces, adding distilled water, heating for extraction, concentrating the extractive solution, centrifuging to remove insoluble substances, adding ethanol, stirring, standing for precipitation;
(2) Centrifuging and collecting the precipitate;
(3) Redissolving the precipitate with distilled water, removing protein, concentrating, and freeze drying.
Preferably, in the step (1),
the material liquid ratio of the starwort root decoction pieces to the distilled water for each extraction is 1 (15-25) w/v.
Preferably, in the step (1),
extracting for 1-3 times for 2-4h.
Preferably, in the step (1),
after the first extraction, filtering and collecting an extracting solution and filter residues; extracting the residue for 1-2 times, and mixing the extractive solutions for concentrating.
Preferably, in the step (1),
the concentration is reduced pressure concentration at 65 ℃;
the final concentration of the ethanol after being added is 80 percent;
the standing precipitation was performed at 4 ℃ and left overnight.
The low-temperature standing is beneficial to the separation of the polysaccharide, and the yield is improved.
Preferably, in the step (2),
the centrifugal speed is 3000-5000rpm, and the time is 10min.
Application of Stellaria polysaccharide in preparing antidepressant is provided.
Has the advantages that:
the Stellaria dichotoma polysaccharide can obviously shorten the immobility time of Forced Swimming (FST) of CUMS rats, the activity score and the exploration score of the rats are both obviously increased without influencing the autonomous activity of the rats, and the sugar water preference rate (SPT) is obviously increased, which shows that the Stellaria dichotoma polysaccharide has stronger antidepressant effect and no central excitation or inhibition effect.
The starwort root polysaccharide can obviously improve the content of Dopamine (DA), 3, 4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), tyrosine Hydroxylase (TH) and tryptophan hydroxylase (TPH) in the brain of a CUMS model rat, and the antidepressant action mechanism of the starwort root polysaccharide is probably related to the regulation of the content of monoamine neurotransmitters in the brain.
The detection of glucocorticoid in rat serum shows that CUMS model rats excessively secrete adrenocortical hormone (ACTH) and Corticosterone (CORT), and the Stellaria dichotoma polysaccharide can restore the function of HPA axis, reverse the rise of ACTH and CORT and restore the ACTH and CORT to be close to normal level; it is revealed that the antidepressant effect of Stellaria dichotoma polysaccharide may be related to the negative feedback function of reconstructing normalized HPA axis.
In conclusion, the Stellaria polysaccharide can be used for preparing the medicine for treating the depression, is derived from Stellaria dichotoma and belongs to nontoxic and harmless natural components, and the medicine prepared from the Stellaria dichotoma does not cause serious side effects, is safe to take and can solve the problem of treating the depression.
Drawings
FIG. 1 shows a standard curve for polysaccharide assay.
FIG. 2 shows the results of a sugar water preference test; n =10, and n is a linear variable of, ** P<0.01 compared to the Control group, # P<0.05、 ## P<0.01 compared to the CUMS group.
FIG. 3 shows the results of forced swim tests; n =10 (m) of the total number of the main chains, ** P<0.01 compared to the Control group, # P<0.05 compared to the CUMS group.
FIG. 4 shows an open field trial activity score; n =10, and n is a linear variable of, ** P<0.01 compared to the Control group, # P<0.05、 ## P<0.01 compared to the CUMS group.
FIG. 5 shows the open field trial exploration score; n =10, and n is a linear variable of, ** P<0.01 compared to the Control group, # P<0.05 compared to the CUMS group.
FIG. 6 shows the results of detection of a monoamine neurotransmitter; n =10, and n is a linear variable of, * P<0.05、 ** P<0.01 compared to the Control group, # P<0.05、 ## P<0.01 compared to the CUMS group.
FIG. 7 shows the results of glucocorticoid detection; n =10, and n is a linear variable of, * P<0.05、 ** P<0.01 compared to the Control group, # P<0.05、 ## P<0.01 compared to the CUMS group.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Taking dried bupleurum root decoction pieces, and mixing the raw materials in a ratio of 1:20 (w/v) adding distilled water, boiling for water extraction for 1h, filtering and collecting an extracting solution and filter residues; extracting the residue for 2 times, each for 1 hr. Mixing extractive solutions, vacuum concentrating at 65 deg.C with vacuum pump connected with rotary evaporator, precipitating with 95% ethanol to final alcohol concentration of 80%, standing at 4 deg.C overnight, centrifuging at 4000rpm for 10min, and collecting precipitate. Redissolving the precipitate with distilled water, removing protein by sevag method, collecting water layer liquid, concentrating, and freeze drying to obtain Stellaria delavayi polysaccharide lyophilized powder.
The polysaccharide content of the starwort root polysaccharide freeze-dried powder is determined by adopting a phenol-sulfuric acid method, a standard curve is shown in figure 1, and the result shows that the polysaccharide content in the starwort root polysaccharide freeze-dried powder is 76.3% +/-0.27 (n = 6).
Example 2
Taking dried bupleurum root decoction pieces, and mixing the raw materials in a ratio of 1:25 (w/v) adding distilled water, boiling for water extraction for 1.5h, filtering and collecting an extracting solution and filter residues; extracting the residue for 1 time for 1.5 hr each time. Mixing extractive solutions, vacuum concentrating at 65 deg.C with rotary evaporator, precipitating with 95% ethanol to final alcohol concentration of 80%, standing overnight at 4 deg.C, centrifuging at 4000rpm for 10min, and collecting precipitate. Redissolving the precipitate with distilled water, removing protein by sevag method, collecting water layer liquid, concentrating, and freeze drying to obtain Stellaria delavayi polysaccharide lyophilized powder.
Example 3
Taking dried starwort root decoction pieces, and mixing the raw materials in a material-liquid ratio of 1:20 (w/v) adding distilled water, boiling and extracting for 1h, and filtering to collect an extracting solution and filter residues; the residue is extracted for 0.5 hr for 2 times. Mixing extractive solutions, vacuum concentrating at 65 deg.C with rotary evaporator, precipitating with 95% ethanol to final alcohol concentration of 80%, standing overnight at 4 deg.C, centrifuging at 4000rpm for 10min, and collecting precipitate. Redissolving the precipitate with distilled water, removing protein by sevag method, collecting water layer liquid, concentrating, and freeze-drying to obtain Stellaria dichotoma polysaccharide lyophilized powder.
Example 4
1. Experimental Material
The starwort root polysaccharide freeze-dried powder prepared in the embodiment 1 is stored at 4 ℃ for later use.
2. Laboratory animal
SPF male SD rat with weight of 200-220g provided by Ningxia medical university laboratory animal center has the qualification number of SCXK (NING) 2020-0001. Animals are raised in a light and dark circulating environment with the humidity of 50 +/-10% and the room temperature of 23 +/-2 ℃, are freely fed with water and are adapted to the environment for one week, and then are used for experiments. All animal experiments in the study are carried out according to the requirements of related national laws and animal ethics and after the approval of animal ethics committees.
3. Animal grouping and establishment of rat unpredictable chronic stress model (CUMS)
A chronic stress rat model induced by chronic unpredictable stimuli was used.
After male SD adaptive feeding for one week, the rats are raised in a single cage, and are randomly divided into 5 groups, namely a Control (blank) group, an FLU (positive drug) group, a starwort root polysaccharide freeze-dried powder low-dose group, a high-dose group and a CUMS (model) group.
Except for the Control group, the rats were housed in separate laboratories, and the remaining 4 groups of rats were housed individually and subjected to 28-day chronic unpredictable mild stimulation molding, including cold water swimming (4 ℃,5 min), tail clamping (1 min), water deprivation (24 h), fasting (24 h), inclined cage rearing (taking out the padding, inclining the cage 45 °), moist rearing (adding 200mL of water per 100g of padding), overnight lighting stimulation, and day-night reversing for 24h; a stress stimulus was administered randomly daily and the same stimulus was not applied continuously.
Wherein, the low and high dose groups are respectively administrated by intragastric administration according to 35mg/kg/d and 150mg/kg/d, the FLU group is administrated by intragastric administration according to 1.8mg/kg/d of fluoxetine hydrochloride, and the Control group and the CUMS group are administrated by normal saline with equal volume.
4. Molding dieAfter 4 weeks of chronic mild stimulation, all rats are subjected to behavioral tests and index detection in turn; the data were counted using SPSS21.0 software, and the results are expressed as X + -S; experimental results the comparisons among groups were first analyzed by One-way ANOVA, and when the differences were significant, statistical analysis was performed by two-sided T-test. # P<0.05、 ## P<0.01， * P<0.05 and ** P<0.01 was significantly different.
1) Sweet water preference test (SPT)
The index is set to simulate the pleasure of human beings and is an effective index reflecting the pleasure of rats.
Rats were trained to drink 1% sugar water for 24h before the start of the experiment, and then given distilled water for 24h, with a normal diet during the training period. After training is finished, the rats are fasted and are forbidden to drink for 12 hours, one bottle of 1% sugar water and one bottle of distilled water are given to the rats, and after 24 hours, the sugar water consumption and the distilled water consumption are measured and recorded.
According to the syrup preference formula: sugar water preference rate% = sugar water consumption (mL)/[ total consumption (sugar water consumption (mL) + pure water consumption (mL) ] × 100% sugar water preference rate was calculated.
The results are shown in fig. 2, and after 28 days of molding, the sugar water preference rate of CUMS rats is significantly reduced compared with that of Control rats ( ** P<0.01 Prove that the molding is successful. After 28 days of continuous administration, the sugar water preference rate of the low dose group was significantly increased compared to that of the CUMS group ( # P<0.05 Sugar water preference rates significantly increased in the high dose and FLU groups: ( ## P<0.01 Sugar water preference results show that the high dose group has better antidepressant action.
2) Forced Swimming Test (FST)
Forced swim tests were used to test the despair behavior of rats.
According to Porsolt's experimental method, rats are first placed in a transparent glass jar 60cm in height and 30cm in diameter, and pre-swim for 5min at a water depth of 25 + -5 cm and a water temperature of 24 + -1 deg.C. And performing formal experiments after 24 hours, wherein the experiments are performed for 6min. The rat tries to struggle to escape initially, but stops struggling after feeling unperceived, only a little movement is carried out to expose the head out of the water, and the limbs float and maintain a motionless state, which shows behavior hopelessness. When the experiment is started, the rat is firstly adaptive to swimming for 2min, the accumulated motionless time of the rat within 4min after the recording is carried out, and the washing and water changing are carried out after each experiment, so that the influence on the next rat test is avoided.
As shown in FIG. 3, the immobility time of the CUMS group rats was significantly prolonged compared to the Control group ( ** P<0.05 ); the high dose group significantly reduced the immobility time of the rats in swimming compared to the CUMS group: ( # P<0.01). The experimental results show that the starwort root polysaccharide shows obvious antidepressant effect.
3) Open Field Test (OFT)
Open field tests are used to test rats for adaptation, satisfaction, exploratory behavior in new and different environments, and for fear, stress and alertness of new environments.
Reference is made to the experimental method of Monteggia with modifications: in the morning in a quiet room. The open field specification is 100cm × 100cm × 40cm, and the activity of the rat in 5min is observed while the rat is placed at the midpoint position during the experiment, including activity score (horizontal cross-cell number, number of crossing into the adjacent cell with three claws or more) and exploration score (vertical standing number, number of crossing into the adjacent cell with two forelimbs 1cm or more). After the detection of each rat is finished, 75% ethanol is used for cleaning an open field to avoid influence of factors such as smell on the activity of the rat, and the experimental test environment should be kept as quiet as possible.
The test results are shown in fig. 4 and 5, and the activity score and the exploration score of the CUMS group rats are significantly reduced compared with the Control group 28 days after the model building (the activity score and the exploration score of the CUMS group rats are significantly reduced) ( ** P<0.01 Indicating that the CUMS-induced activity of the rat in the depression model is obviously reduced, and the exploration of unknown environment is reduced; after continuous administration, the activity score and the exploration score of the high dose group were significantly increased: ( # P<0.05； ## P<0.01 Experimental results show that the high-dose group has obvious antidepressant effect.
4) Influence of Stellaria dichotoma polysaccharide on monoamine neurotransmitters of CUMS model rats
Monoamine neurotransmitter detection: after 1h of dosing, the rats were immediately decapitated and the entire brain, including hippocampus and frontal cortex, was removed. The cells were transferred rapidly and treated with ice saline, 1mL of saline was added to each tissue sample, homogenized with a homogenizer to make hippocampal and cortical homogenates, and centrifuged (16000 rpm) at 4 ℃ for 10min. Taking supernatant, and respectively measuring the levels of Dopamine (DA), 3, 4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), tyrosine Hydroxylase (TH) and tryptophan hydroxylase (TPH) according to the operation instruction of an enzyme-linked immunosorbent assay (ELISA) kit.
The average contents of neurotransmitter-like components and related metabolic enzymes in hippocampus (hippopampus) and cortex (prefrontal core) of rats in the CUMS group and each administration group are shown in FIG. 6. Compared with the Control group, the concentrations of TH,5-HT,5-HIAA, TPH, DA and DOPAC in hippocampus and cortex of rats in the CUMS group are all significantly reduced ( * p<0.05， ** p<0.01 ); the related components had a tendency of significant callback in both the administered group compared to the CUMS group, in which TH in hippocampus and cortex was significantly higher in the high dose group than in the CUMS group (c # p<0.05 ); 5-HT and 5-HIAA in hippocampus and cortex are significantly higher in the high dose group and FLU group than in CUMS group () # p<0.05 ); TPH and DA were significantly higher in hippocampus than in model group in high dose and FLU groups ((II)) # p<0.05； ## p<0.01 ); TPH and DA in cortex were significantly higher in the high dose group than in the model group ( # p<0.05 ); the content of DOPAC in hippocampus was significantly higher in the high dose group than in the CUMS group ( # p<0.05)。
5) Effect of Stellaria dichotoma polysaccharide on glucocorticoid of rat
And (3) glucocorticoid detection: rat serum samples were taken, thawed at room temperature, and the levels of adrenocorticotropic hormone (ACTH) and Corticosterone (CORT) in rat serum were determined separately according to the protocol in the ELISA kit.
As shown in FIG. 7, compared with the Control group, the CUMS group significantly increased the levels of ACTH and CORT in the serum, while the Bupleurum scorzonerifolium polysaccharide lyophilized powder low dose group, the high dose group and the FLU group significantly decreased the levels of ACTH and CORT in the serum of rats (C) # p<0.05； ## p<0.01)。
In conclusion, the starwort root polysaccharide freeze-dried powder has the pharmacological activity of resisting depression, and the action mechanism of the starwort root polysaccharide freeze-dried powder is probably related to the regulation of the content of monoamine neurotransmitters in the brain and the reconstruction of the normalized HPA axis negative feedback function.
The general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (2)
1. The application of the starwort root polysaccharide freeze-dried powder in the preparation of antidepressant drugs is characterized in that,
the starwort root polysaccharide freeze-dried powder is prepared by taking starwort roots as raw materials and performing water extraction and alcohol precipitation, collecting precipitates, redissolving to remove protein, concentrating and freeze-drying;
the preparation method of the starwort root polysaccharide freeze-dried powder comprises the following steps:
(1) Taking radix Stellariae decoction pieces, adding distilled water, heating for extraction, concentrating the extractive solution, centrifuging to remove insoluble substances, adding ethanol, stirring, standing for precipitation;
(2) Centrifuging and collecting the precipitate;
(3) Redissolving the precipitate with distilled water, removing protein, concentrating, and freeze drying;
in the step (1), the step (c),
the material liquid ratio of the starwort root decoction pieces to the distilled water for each extraction is 1 (15-25) w/v; extracting for 1-3 times for 2-4h;
the concentration is reduced pressure concentration at 65 ℃; the final concentration of the added ethanol is 80%; the standing precipitation is carried out at 4 ℃;
in the step (2), the step (3),
the centrifugal speed is 3000-5000rpm, and the time is 10min.
2. The use according to claim 1,
in the step (1), the step (c),
after the first extraction, filtering and collecting an extracting solution and filter residues; extracting the residue for 1-2 times, and mixing the extractive solutions for concentrating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110138841.9A CN112843086B (en) | 2021-02-01 | 2021-02-01 | Polysaccharide freeze-dried powder with anti-depression effect and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110138841.9A CN112843086B (en) | 2021-02-01 | 2021-02-01 | Polysaccharide freeze-dried powder with anti-depression effect and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112843086A CN112843086A (en) | 2021-05-28 |
| CN112843086B true CN112843086B (en) | 2022-11-08 |
Family
ID=75987543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110138841.9A Active CN112843086B (en) | 2021-02-01 | 2021-02-01 | Polysaccharide freeze-dried powder with anti-depression effect and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112843086B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115819634B (en) * | 2022-11-23 | 2024-01-26 | 宁夏大学 | Radix bupleuri polysaccharide and preparation method and application thereof |
| CN115677874B (en) * | 2022-11-23 | 2024-01-23 | 宁夏大学 | Radix bupleuri crude polysaccharide with prebiotic activity and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104666328A (en) * | 2015-02-02 | 2015-06-03 | 西北农林科技大学 | Application of traditional Chinese medicine polysaccharide in medicine and extraction method of traditional Chinese medicine polysaccharide |
| CN105963314A (en) * | 2016-06-28 | 2016-09-28 | 华侨大学 | Application of dendrobium officinale polysaccharides to preparation of antidepressant medicines and antidepressant healthcare products |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006327970A (en) * | 2005-05-25 | 2006-12-07 | Daicho Kikaku:Kk | Digestive apparatus agent and anti-stress agent |
| CN104490972A (en) * | 2015-01-06 | 2015-04-08 | 山西大学 | Pharmaceutical composition for treating and preventing depression and preparation method of pharmaceutical composition |
| CN111777692B (en) * | 2020-08-17 | 2022-02-08 | 山东聚胜生物科技有限公司 | Rubus parvifolius graded polysaccharide, preparation method and application in depression resistance |
-
2021
- 2021-02-01 CN CN202110138841.9A patent/CN112843086B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104666328A (en) * | 2015-02-02 | 2015-06-03 | 西北农林科技大学 | Application of traditional Chinese medicine polysaccharide in medicine and extraction method of traditional Chinese medicine polysaccharide |
| CN105963314A (en) * | 2016-06-28 | 2016-09-28 | 华侨大学 | Application of dendrobium officinale polysaccharides to preparation of antidepressant medicines and antidepressant healthcare products |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112843086A (en) | 2021-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6416795B1 (en) | Herbal extract composition for stress prevention and treatment | |
| CN112843086B (en) | Polysaccharide freeze-dried powder with anti-depression effect and preparation method and application thereof | |
| CN105920476B (en) | Traditional Chinese medicine composition for preventing and treating Alzheimer disease and preparation method thereof | |
| CN103751707B (en) | Oral liquid of a kind of great burdock achene beauty treatment and eliminating spot and preparation method thereof | |
| KR100699790B1 (en) | Pharmaceutical composition for the prevention and treatment of liver disease comprising a Lonicera caerulea L. var. edulis extract | |
| CN114766672A (en) | Old lobular broadleaf holly leaf extract and application thereof in preparing food for relieving alcohol-induced liver injury | |
| CN104363773B (en) | For treating herbal-composition of gastroenteritis disease and its production and use | |
| CN113197315B (en) | A food composition for improving male sexual function, and its preparation method | |
| KR20060104407A (en) | Herbal composition for treating hyperlipemia | |
| KR20130122834A (en) | Korean herb tea composing of extract from spring onion root and method preparing thereof | |
| JP3628999B2 (en) | Anonymous tea and its manufacturing method | |
| CN109430854B (en) | Health product and preparation method thereof | |
| CN1738633A (en) | Anti-obesity ingretients from medicinal plants and their composittion | |
| CN104435773A (en) | Traditional Chinese medicine preparation for treating tetanus | |
| CN107712890A (en) | A kind of antitoxic heart-soothing and sedative cream taste and preparation method thereof | |
| JP2004352626A (en) | Anticholesterol agent containing plant-derived component | |
| CN104208683B (en) | The pharmaceutical composition of a kind of anti-duck viral disease, lyophilized powder, preparation method and application | |
| KR20050096742A (en) | Extract of nelumbinis semen for the treatment of depression, medicinal composite and health foods including the extract of nelumbinis semen | |
| CN109939211B (en) | Traditional Chinese veterinary medicine preparation for treating chicken colibacillosis and preparation method thereof | |
| WO2013115534A1 (en) | Composition for preventing or treating multiple sclerosis | |
| CN112972483A (en) | Application of morinda officinalis oligosaccharide in preparation of medicine for treating body dysfunction caused by altitude anoxia | |
| CN111265600A (en) | Application of jackfruit extract in preparing weight-reducing preparation | |
| CN106692711A (en) | Traditional Chinese medicine drug granule of nestling initial drug and preparation method thereof | |
| CN116832066B (en) | Application of cuttlefish ink extract in preparing medicine for treating hyperkinetic syndrome and depression | |
| CN114931214B (en) | Chinese herbal medicine composition for improving hearing impairment and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |