CN112840917A - Tissue culture test box for researching multi-photon regulation and control of plant growth and use method - Google Patents

Tissue culture test box for researching multi-photon regulation and control of plant growth and use method Download PDF

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Publication number
CN112840917A
CN112840917A CN202011611565.5A CN202011611565A CN112840917A CN 112840917 A CN112840917 A CN 112840917A CN 202011611565 A CN202011611565 A CN 202011611565A CN 112840917 A CN112840917 A CN 112840917A
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box
box body
tissue culture
development
small
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撒刚
夏露露
袁剑龙
王玉萍
张峰
程李香
余斌
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Gansu Agricultural University
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Gansu Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/14—Greenhouses
    • A01G9/16—Dismountable or portable greenhouses ; Greenhouses with sliding roofs
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
    • A01G9/246—Air-conditioning systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
    • G01D21/00—Measuring or testing not otherwise provided for
    • G01D21/02—Measuring two or more variables by means not covered by a single other subclass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098—Plants or trees
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14—Measures for saving energy, e.g. in green houses

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Forests & Forestry (AREA)
  • Medicinal Chemistry (AREA)
  • Ecology (AREA)
  • Analytical Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a tissue culture test box for researching multi-light quality regulation and control of plant growth and development.A box body is internally provided with 9 rows of small lamp boxes, two side walls in the small lamp boxes are symmetrically provided with a plurality of rows of longitudinal seedling tray buckles, and seedling trays can be lapped on the seedling tray buckles on two sides; the top in the small lamp box is provided with a plurality of LED lamps, and the small lamp box is internally provided with a temperature sensor, a humidity sensor and an illumination intensity sensor; the rear wall of the small box body is provided with a plurality of rows of box body rear side ventilation openings, and a box body back air outlet is arranged on the rear wall between the upper layer and the lower layer of the small box body. The invention provides a method for quickly and accurately obtaining test data, which comprises the following steps: the time used by the test is greatly shortened, meanwhile, the consistency of other environmental factors in the test is kept, and the rapidness, consistency and accuracy of obtaining test data are ensured.

Description

Tissue culture test box for researching multi-photon regulation and control of plant growth and use method
Technical Field
The invention belongs to the technical field of light-controlled plant growth and development, and particularly relates to a tissue culture test box for researching multi-light quality-controlled plant growth and development.
Background
Light, one of the important environmental factors affecting plant growth and development, is the main energy source for plant photosynthesis. Plays an important role in all stages of plant growth. The influence of light on the growth and development of plants is mainly reflected in three aspects of illumination intensity (Irradiance), illumination time (Photoperiod) and light quality (Lightqualit). The response of plants to changes in light quality is more complex than the intensity and duration of the light. The influence of light on the growth and development, morphological structure, photosynthetic property and physiological metabolism of plants has been an important subject in the field of plant photobiology. Therefore, the research on the differences of the light quality, the light intensity and the illumination time has very important significance in the aspects of researching the growth and development mechanism of plants, improving the yield and the quality of crops and the like.
With the development of the photoelectric technology, a Light-emitting diode (LED) as a fourth-generation novel illumination Light source not only has the advantages of energy saving, environmental protection, high photoelectric conversion efficiency, long service life, low heat generation, small cooling load, and adjustable combination of illumination intensity, Light quality and frequency spectrum, but also has become an important technical means for researchers to study the regulation and control of plant growth and development, photosynthesis and metabolism.
At present, the agricultural production mode of China is gradually changed from a traditional agriculture mode to a facility agriculture mode, and is gradually changed to an industrial cultivation mode. Plant factories can exploit artificial light regulation to positively influence plants and crops, thereby increasing crop productivity and throughput. Therefore, the basic requirement of crops on light is met, the production of fruits and vegetables is not influenced by weather, the vegetable waste is avoided, the threat of plant diseases and insect pests is avoided, and the requirement of modern people on healthy diet is met. Therefore, the reasonable manual regulation and control of the light has important significance on the growth of plants.
The study of suitable lighting conditions is very important for the development of "facility agriculture". However, in the current industrial production system, the limitation of non-uniform environmental factors such as temperature, humidity and ventilation is imposed, and the limitation of finding suitable lighting conditions by accurate test conditions affects the unrepeatability and accuracy of test results. The investment of manpower and material resources is large, and the production is wasted, so that the production cost of an enterprise is increased, and the development of the enterprise is not facilitated. Meanwhile, the uncontrollable environmental factors in factory are too many, so that serious interference can be caused to the research on the aspect of light regulation and control of plant growth and development mechanism, if the laboratory assembly culture technology can be utilized to integrate the factory facility agriculture concept into the controllable laboratory condition, the cost problem of enterprise research can be greatly reduced, and the problems that the factory condition is uncontrollable in the aspect of research on the basis of light regulation and control of plant growth and development, the laboratory space is small, and the number of personnel is small can be solved.
The research cost in the aspect of optimizing the light-regulated crop production technology in factory is reduced by combining the facility agriculture concept with the tissue culture technology under the laboratory condition, and the problem of environment condition uniformity culture in the research of scientific problems such as light-regulated plant growth and development, light-regulated crop yield and quality in the laboratory is solved. The key point of the technology is that controllable tests of single light quality, single light intensity, single light time processing and the like can be realized in a laboratory under the uniform conditions of environmental interference factors such as natural light, temperature, humidity, ventilation and the like, so that the repeatability and the accuracy of the tests are better ensured.
There is no such apparatus and method to achieve this. If the device is manufactured, the equipment ensures that the interference environment in the whole test process is uniform; how to realize that the light quality can be adjusted independently; how to realize that the illumination intensity can be adjusted independently; how to realize the comprehensive regulation and control such as independent adjustment of illumination time. These problems need to be solved.
The problems of the prior art are as follows: the prior art does not have a multi-light-quality and multi-function integrated illumination tissue culture device specially used for scientific research in a laboratory, and a common light control device is produced in an industrial manner, so that the operation cost is high, the condition controllability is poor, and the requirement of scientific research in the laboratory cannot be met. In addition, in basic scientific problems such as light control of plant growth and development in laboratory research, facilities such as a large-scale illumination culture room are needed, and the operation is difficult for laboratories with short test lands; moreover, it is very difficult to achieve the uniform control of environmental interference factors in a large-scale illumination culture room, and the cost is very large. Therefore, the patent aims to develop a multi-light and multi-functional test device for scientific research in a laboratory and a method for quickly and accurately obtaining test data. The device can well solve the difficult problems of uncontrollable and high cost in the process of researching the condition optimization in the aspects of light-controlled crop yield and quality, further researches the molecular mechanisms of morphological construction, photosynthetic property and physiological metabolism of factors such as light quality, light intensity, light illumination time and the like for controlling different growth and development stages of plants in the future, and provides scientific basis and reference examples for the application in facility cultivation.
Disclosure of Invention
In order to solve the technical problems, the invention provides a tissue culture test box for researching multi-light quality regulation and control of plant growth and development, which comprises a rectangular box body, wherein the box body is provided with a box door, the upper part in the box body is provided with three rows of 9 small lamp boxes, the small lamp boxes are provided with small box doors, and the small box doors are provided with test box door handles; the box door is provided with a box door observation port made of transparent materials, the box door observation port is in a pocket shape, and a label can be placed in the pocket so as to shield the light of the column; a plurality of rows of longitudinal seedling tray buckles are symmetrically arranged on two side walls in the small lamp box, and the seedling trays can be lapped on the seedling tray buckles on the two sides; the top in the small lamp box is provided with a plurality of LED lamps, and the small lamp box is internally provided with a temperature sensor, a humidity sensor and an illumination intensity sensor; the rear wall of the small box body is provided with a plurality of rows of box body rear side ventilation openings, the rear wall between the upper layer and the lower layer of the small box body is provided with a box body back air outlet, and the box body rear side ventilation openings and the box body back air outlet are communicated with a temperature, humidity and ventilation adjusting mechanism arranged at the lower part of the box body through an air channel.
Preferably, the temperature, humidity and ventilation adjusting mechanism comprises a compressor, an electric heater, a humidifier and a fan, wherein an air return pipe for adjusting air conditions is arranged at the lower part in the box body, and the air return pipe is communicated with the upper part of the box body for air circulation; a cooling cover and a heating sleeve are arranged on the air return pipeline, the compressor is communicated with the cooling cover through a refrigeration pipeline, and the electric heater is connected with the heating cover through a circuit; the spray opening of the humidifier is communicated with the air return opening; the return air pipe is provided with a fan used for driving air circulation.
Preferably, the device also comprises a control box arranged at the lower part in the box body, and the control box is connected with a temperature sensor, a humidity sensor and an illumination intensity sensor through signal lines; the control box is respectively connected with the compressor and the control switch of the ultrasonic generator through control circuits.
Preferably, an LED lamp control switch is arranged on the inner side wall of the small lamp box.
Preferably, the box door is a split rotary door, and the door handles are arranged on the box doors on two sides.
Preferably, a touch display screen is arranged on the box door.
Preferably, a power indicator lamp is arranged below the front of the box body.
Preferably, the rear side ventilation opening of the box body is formed by punching a plurality of horizontal notches in a metal plate.
Preferably, the width of the box body is 1580mm, and the height of the box body is 2240 mm.
The use method of the tissue culture test box for researching multi-photon regulation and control of plant growth and development comprises the following steps: firstly, the temperature, the humidity and the ventilation performance of the whole test environment are set as required through a large box body; then, setting the light quality, the illumination intensity and the illumination time of each small lamp box; finally, respectively putting the tissue culture seedlings of the plants into small lamp boxes for illumination treatment; and after the treatment is finished, performing physiological and biochemical and molecular biological detection as required to obtain a test result.
The invention has the patent innovation points that:
1. the method for quickly and accurately obtaining the test data is provided: in the invention, a test device comprising 9 groups of independently controllable lamp boxes is designed, 9 groups of treatments can be carried out in each test, and the test environments (temperature, humidity, ventilation and the like) of the 9 groups of treatments can be kept consistent through setting. Therefore, the time for the test is greatly shortened, the consistency of other environmental factors in the test is kept, and the rapidness, consistency and accuracy of obtaining test data are ensured. In the prior experiments related to the growth and development of plants treated by multiple light qualities, a single light box is mostly used, and the treatment groups of each experiment are fewer, so that the experiments need to be carried out for multiple times, the risk of inconsistency of other environmental factors exists in each experiment, the consistency of the experiment conditions is influenced, and the accuracy of data is influenced. Moreover, the multiple tests also increase the personnel cost, the time cost and the economic cost, and the failure rate of the tests is higher. And a plurality of groups of treatments are carried out on the culture shelf, but the tests on the culture shelf have the problems of too many environmental interference factors, larger occupied space, high cost and the like, and are difficult to realize in smaller laboratories.
2. A test method for multifunctional operation is provided: the nine small boxes are unified independently, the LED lamp panels in each box can be simply replaced, and the illumination time and the illumination intensity in each box can also be set independently. During the test, the temperature, the humidity, the ventilation performance and the like of the whole test environment can be set as required through the large box body, and then the light quality, the illumination intensity and the illumination time of each small lamp box are set. The test method can be set according to different test requirements of different testers in the test, and can also be used for carrying out cross setting on environmental factors, light quality, light wavelength and light intensity, so that the test method which is beneficial to the growth and development of different plants among different cross treatments is obtained. The invention can basically meet all test items related to illumination treatment, and has wide application range and stronger test performance comprehensiveness.
3. Simple operation, practicality are strong: compared with the prior light treatment test, the invention has the advantage of very convenient operation in the light test. The whole equipment box body is small, does not occupy too large laboratory space, and is convenient to move and place. Meanwhile, the whole machine and 9 independent lamp boxes can be controlled independently, and the digital setting is simple, quick, easy to operate and high in practicability.
Drawings
FIG. 1 is a perspective view of an open cabinet door of a tissue culture test box for studying growth and development of multi-light quality control plants.
FIG. 2 is a perspective view of the light quality tissue culture test box with the small cabinet door opened.
FIG. 3 is a front elevation view of the light quality tissue culture test chamber.
FIG. 4 is a side elevation view of the light quality tissue culture test chamber.
FIG. 5 is a front view of an open cabinet door of the light tissue culture test chamber.
FIG. 6 is a cross-sectional view of a small box body of the light quality tissue culture test box.
FIG. 7 is a back view of a small box of the light quality tissue culture test box.
FIG. 8 is a schematic view of the upward opening of the small box body of the light quality tissue culture test box.
FIG. 9 is a front sectional view of the light tissue culture test chamber.
FIG. 10 is a side sectional view of the light tissue culture test chamber.
In the figure, 1-door handle of test box, 2-place of label (door viewing port), 3-air outlet at back of box, 4-LED lamp, 5-air vent at back of box (stamping part), 6-seedling tray buckle, 7-seedling tray, 8-touch display screen, 9-door handle, 10-power indicator lamp, 11-LED control switch, 12-compressor, 13-humidifier, 14-fan, 15-heater.
Detailed Description
As shown in figures 1-10, the invention discloses a tissue culture test box for researching growth and development of multi-light regulation plants, the tissue culture test box is of a cuboid structure, the width of the box body is 1580MM, the height of the box body is 2240MM, the box body is of a middle double-door structure, two sides of the box door are provided with oppositely-pulling door handles 9, one side of the box door is provided with a touch display screen 8, and a power indicator lamp 10 is arranged below the front side of the box body. The upper row and the lower row in the box are provided with 9 small lamp boxes, the small lamp boxes are provided with box doors, the box doors are provided with test box door handles 1 and pulling door handles, the box doors can rotate outwards around hinges at the bottoms, the box doors are provided with box door observation ports 2, the box door observation ports 2 are pocket-shaped and made of transparent materials, and labels can be placed in the pockets so as to shield the column light.
A plurality of rows of longitudinal seedling tray buckles 6 are symmetrically arranged on two side walls in the small lamp box, and the seedling trays 7 can be lapped on the seedling tray buckles 6 on two sides and are similar to a partition plate structure of a refrigerator; the top in the small box body is provided with a plurality of LED lamps 4, the rear wall of the small box body is provided with a plurality of box body rear side ventilation openings 5, the box body rear side ventilation openings 5 are formed by punching a plurality of horizontal notches on a metal plate, and the inner side wall of the small box body is provided with an LED lamp control switch 11.
The inner wall of the small lamp box is provided with reflective paper or a reflective plate, and the reflective paper can be made of PET.
The rear wall of the middle of the upper and lower layers of small boxes is provided with a box back air outlet 3, and the lower part of the small box in the box is provided with a compressor 12, a fan 14, a humidifier 13 and a heater 15.
The lower part of the box body comprises an air return pipe used for adjusting air conditions, and the air return pipe is communicated with the upper part of the box body for air circulation; a cooling cover and a heating sleeve are arranged on the air return pipeline, a compressor is arranged in the lower side box body and is communicated with the cooling cover through a refrigeration pipeline, and an electric heater in the lower side box body is connected with the heating cover through a circuit; the lower part in the box body is also provided with a humidifier, and a spray nozzle of the humidifier is communicated with the return air inlet; the return air pipe is provided with a fan used for driving air circulation.
The adjusting mechanism also comprises a control box arranged at the lower part in the box body, a temperature sensor, a humidity sensor and an illumination intensity sensor which are connected with the control box through signal lines are arranged in the small lamp box, and the control box can independently adjust the light quality, the illumination intensity and the illumination time; the control box is respectively connected with the control switches of the compressor and the humidifier through control circuits.
Example 1
Experiment for regulating plant growth and development by different light qualities:
in the experiment of plant growth and development controlled by different light qualities, the LED lamp panels with different light qualities required by the experiment are replaced, and 9 treatment wavelengths, such as full spectrum white, 390nm, 435nm, 470nm, 525nm, 570nm, 620nm, 670nm and 780nm, can be set according to the experiment requirements. Then, the illumination time and the illumination intensity of the 9 treatment boxes are individually set, for example, the illumination time of each of the 9 treatment boxes is set to 16:8h, and the illumination intensity is uniformly set to 4500 lx. Setting the whole test environment on a large box body, such as temperature, humidity and ventilation, and finally respectively putting tissue culture seedlings of arabidopsis thaliana or other plants into small lamp boxes for illumination treatment. And after the treatment is finished, performing physiological and biochemical and molecular biological detection as required to obtain a test result.
Example 2
Experiment for regulating and controlling plant growth and development by different light intensities:
in the experiment of plant growth and development controlled by different light intensities, the same light quality LED lamp panel required by the experiment is replaced firstly, and 9 illumination intensity gradients can be set according to the experiment requirements, such as 500lx, 1000lx, 1500lx, 2000lx, 3500lx, 5000lx, 8000lx, 10000lx and 20000 lx. Then, the spectral wavelength and the illumination time of the 9 processing boxes are individually set, for example, the illumination time of the 9 processing boxes is set to be 16:8h, and the light quality is uniformly set to be full-spectrum white. Setting the whole test environment on a large box body, such as temperature, humidity and ventilation, and finally respectively putting tissue culture seedlings of arabidopsis thaliana or other plants into small lamp boxes for illumination treatment. And after the treatment is finished, performing physiological and biochemical and molecular biological detection as required to obtain a test result.
Example 3
Experiments for regulating and controlling plant growth and development at different illumination time:
in the experiment of regulating and controlling the growth and development of plants at different light times, the same light quality LED lamp panel required by the experiment is replaced at first, and 9 illumination time gradients can be set according to the experiment requirement, for example, 30min, 1h, 2h, 4h, 6h, 8h, 12h, 24h and 48 h. Then, the spectral wavelength and the illumination intensity of the 9 processing boxes are individually set, for example, the illumination intensity of the 9 processing boxes is set to 5000lx, and the light quality is uniformly set to full spectrum white. Setting the whole test environment on a large box body, such as temperature, humidity and ventilation, and finally respectively putting tissue culture seedlings of arabidopsis thaliana or other plants into small lamp boxes for illumination treatment. And after the treatment is finished, performing physiological and biochemical and molecular biological detection as required to obtain a test result.
Therefore, by the experimental device and the method, various experiments related to the light-controlled plant growth and development can be carried out in a laboratory, and the method has the advantages that: the LED lamp panel light intensity and illumination time adjusting device has the advantages that controllable test conditions can be guaranteed, illumination conditions can be controllably adjusted according to different research subjects, test data can be rapidly and accurately obtained, simple replacement of the LED lamp panel and independent adjustment of light intensity and illumination time can be achieved, the laboratory is portable in use, comprehensive in function, low in input cost and high in operability.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. A tissue culture test box for researching multi-light quality regulation and control of plant growth and development is characterized by comprising a rectangular box body, wherein the box body is provided with a box door, the upper part in the box body is provided with three rows of 9 small lamp boxes, the small lamp boxes are provided with small box doors, and test box door handles are arranged on the small box doors; the box door is provided with a box door observation port made of transparent materials, the box door observation port is in a pocket shape, and a label can be placed in the pocket so as to shield the light of the column; a plurality of rows of longitudinal seedling tray buckles are symmetrically arranged on two side walls in the small lamp box, and the seedling trays can be lapped on the seedling tray buckles on the two sides; the top in the small lamp box is provided with a plurality of LED lamps, and the small lamp box is internally provided with a temperature sensor, a humidity sensor and an illumination intensity sensor; the rear wall of the small box body is provided with a plurality of rows of box body rear side ventilation openings, the rear wall between the upper layer and the lower layer of the small box body is provided with a box body back air outlet, and the box body rear side ventilation openings and the box body back air outlet are communicated with a temperature, humidity and ventilation adjusting mechanism arranged at the lower part of the box body through an air channel.
2. The tissue culture test box for researching the growth and development of the multi-light quality regulation and control plant as claimed in claim 1, wherein the temperature, humidity and ventilation regulating mechanism comprises a compressor, an electric heater, a humidifier and a fan, a return air pipe for regulating air conditions is arranged at the lower part in the box body, and the return air pipe is communicated with the upper part of the box body for air circulation; a cooling cover and a heating sleeve are arranged on the air return pipeline, the compressor is communicated with the cooling cover through a refrigeration pipeline, and the electric heater is connected with the heating cover through a circuit; the spray opening of the humidifier is communicated with the air return opening; the return air pipe is provided with a fan used for driving air circulation.
3. The tissue culture test box for researching the growth and development of the multi-light quality regulation and control plant according to claim 1, characterized by further comprising a control box arranged at the lower part in the box body, wherein the control box is connected with a temperature sensor, a humidity sensor and an illumination intensity sensor through signal lines; the control box is respectively connected with the compressor and the control switch of the ultrasonic generator through control circuits.
4. The tissue culture test box for researching the growth and development of the multi-light quality regulation and control plant of claim 1, wherein the inner wall of the small lamp box is provided with a reflective paper or a reflective plate, and the inner side wall of the small lamp box is provided with an LED lamp control switch.
5. The tissue culture test box for studying growth and development of plants under multiple light qualities according to claim 1, wherein the box door is a split rotating door, and door handles are arranged on the box doors at two sides.
6. The tissue culture test box for researching growth and development of multi-light quality regulated plants according to claim 1, wherein a touch display screen is arranged on the box door.
7. The tissue culture test box for researching plant growth and development regulated by multiple light qualities of claim 1 is characterized in that a power indicator lamp is arranged below the front of the box body.
8. The tissue culture test box for studying the growth and development of plants with multiple light qualities according to claim 1, wherein the ventilation opening at the rear side of the box body is formed by punching a plurality of horizontal notches on a metal plate.
9. The tissue culture test box for researching growth and development of the multi-light quality regulation and control plant of claim 1, wherein the width of the box body is 1580mm, and the height of the box body is 2240 mm.
10. The use method of the tissue culture test box for researching the growth and development of the multi-light quality regulation and control plant as claimed in claims 1-9 is characterized in that the temperature, the humidity and the ventilation performance of the whole test environment are set as required through a large box body; then, setting the light quality, the illumination intensity and the illumination time of each small lamp box; finally, respectively putting the tissue culture seedlings of the plants into small lamp boxes for illumination treatment; and after the treatment is finished, performing physiological and biochemical and molecular biological detection as required to obtain a test result.
CN202011611565.5A 2020-12-30 2020-12-30 Tissue culture test box for researching multi-photon regulation and control of plant growth and use method Pending CN112840917A (en)

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* Cited by examiner, † Cited by third party
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CN113647279A (en) * 2021-09-10 2021-11-16 内蒙古科技大学包头师范学院 Facility for short-lived plant adaptive evolution research
CN114258805A (en) * 2021-12-30 2022-04-01 武汉亘谷源生态农业科技有限公司 Test device for rice field seedling growth and recording method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113647279A (en) * 2021-09-10 2021-11-16 内蒙古科技大学包头师范学院 Facility for short-lived plant adaptive evolution research
CN114258805A (en) * 2021-12-30 2022-04-01 武汉亘谷源生态农业科技有限公司 Test device for rice field seedling growth and recording method thereof

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