CN103384720A - Biological culturing device with complete wavelength light-control - Google Patents
Biological culturing device with complete wavelength light-control Download PDFInfo
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- CN103384720A CN103384720A CN2012800044808A CN201280004480A CN103384720A CN 103384720 A CN103384720 A CN 103384720A CN 2012800044808 A CN2012800044808 A CN 2012800044808A CN 201280004480 A CN201280004480 A CN 201280004480A CN 103384720 A CN103384720 A CN 103384720A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
A biological culturing device with complete wavelength light-control comprises a box body (12) which is provided with a heat insulating layer on the outside, light wavelength controllable LED illumination panels which are arranged on the inner top side and four lateral sides of the box body (12), a temperature and humidity sensor which is arranged in the box body (12), a heater (7a), a humidifier (8) and a miniature mute compressor (7b) which are fixed on the bottom of the box body (12). Biologic photo-reaction researches can be performed in plant tissue culture, cell culture, microbiological culture and animal culture ways in the culturing device provided with complete wavelength light-control and quartz culture flask (13). The operation is simple. The light source can be subjected to wavelength combination and illumination time program setting according to different requirements.
Description
A kind of organism culturing device the technology of the present invention of Full-wavelength light source controllable is related to biological culture field, more particularly to a kind of vegetable material of Full-wavelength light source controllable（Including callus and seedling）Culture apparatus, it is adaptable to which illumination controls biological cell or tissue or plant light reaction research, physiological metabolism research or animal light reaction physiological Study.
Background
Substantial amounts of scientific research is proved：Nutrient growth and secondary metabolite accumulation are all by the orientation adjustment of wavelength under the light source irradiation of certain wavelength for autotroph cell, and heterotrophic organism cell metabolic pathway under the stimulation of special wavelength light source can also change and cause the accumulation of some bioactive substances.This feature of living cells can be used for the bioactive substance or medicine of industrialized production human needs.Because the light source of different wave length all possesses certain energy, and can only be by some special phytochromes or other photoactive substances in living cells（Such as some enzymes or non-enzymatic protein acceptor）Absorb and gene expression or corresponding metabolism network is changed, thus cause some specific secondary metabolite expression quantity increases.This is the theoretical foundation of apparatus of the present invention.Utilize this characteristic of living cells, light sources with different wavelengths can be in depth studied with system to organism or histiocytic physiological activity, the Regulation Mechanism of culture cell metabolism network, these unknown light reaction mechanism are the important contents that bio-light reacts scientific research.
The light irradiation of various wavelength is broken up to plant growth, the metabolic, physiological activity of animal and the intracellular complicated metabolism network of culture have certain photochemical effect, so as to have regulatory function to signal transduction, the activity of enzyme and gene expression, this by bio-light reaction theory and by cell culture for the genetic engineering industry for the purpose of means production bioactive substance and medicine to being significant.The especially tissue cultures of rare medicinal plant or cultivation or cell fermentation light reaction research is significant, but does not meet the scientific instrument of this research needs still at present.
The content of the invention
The purpose of the present invention is the above mentioned problem existed for existing technology, it is to be the provision of a kind of organism culturing device of Full-wavelength light source controllable, the device is provided from 200 nanometers to the all-wave length light source of 1000 nanometer ranges and LED daylight lamp light source, the need for scientific research, light source controller on the device can select the light of any wavelength in the wave-length coverage to be combined to obtain the mixing light source or monochromatic source of special wavelength combination, light reaction research is carried out to fermented cells or plant callus or plant seedlings or meiofauna using this light source.It is particularly suitable for use in：Expression regulation of the illumination of specific wavelength to some genes, the regulation and control accumulated to secondary metabolite, to the research in terms of plant cell growth, the regulation and control of differentiation, physiological activity, the regulation and control of metabolism to plant influence on the physiological activity of meiofauna.
The purpose of the present invention can be realized by following technical proposal：
A kind of organism culturing device of Full-wavelength light source controllable, including casing, blake bottle, it is characterised in that：
In casing：
First face solid LED illumination panel controller, second face solid LED illumination panel controller, 3rd face solid LED illuminating panel controllers, fourth face solid LED illumination panel controller, 5th face solid LED illumination panel controller, temperature, humidity and ultraviolet lamp controller are separately fixed at the inner side of casing top panel, are from left to right arranged in order.
First face solid LED illumination panel controller is connected with the three-dimensional LED illumination panel controller in the second face, second face solid LED illumination panel controller is connected with the three-dimensional LED illumination panel controller in the 3rd face, 3rd face solid LED illumination panel controller is connected with the three-dimensional LED illumination panel controller of fourth face, fourth face solid LED illumination panel controller is connected with the three-dimensional LED illumination panel controller in the 5th face, and the 5th face solid LED illumination panel controller is connected with temperature, humidity and ultraviolet lamp controller；Each the numerical monitor and parameter setting panel of controller are exposed and are located at each self-corresponding position in top of chamber door.
Heater, compressor, three parts of humidifier are separately fixed on box bottom.
LED light source plate is fixed on corresponding cabinet panel inwall；Inwall panel（Quartzy panel）It is covered on LED light source plate；Other four sides inwalls are adopted in casing is set in a like fashion.
First valve is fixed on above casing top panel, and the passage of the first valve runs through casing top in favor of ventilation in vertical position；Second valve is the gas valve on box bottom, and the second valve also runs through bottom half in vertical position, and the first valve and the second valve are used to ventilate in case.
First chamber door is fixed on the panel on the casing left side, and the second chamber door is fixed on the panel on the right of casing, and two chamber doors are split；First boxboard is arranged on 1/2nd height in casing, and the second boxboard is arranged on the vertical center position of left and right directions in casing.
It is highly 5-20 centimetres that blake bottle, which includes quartzy bottle, quartzy bottle cap, sterile breather plug socket, quartzy bottle, a diameter of 3-20 centimetres, it can also be needed according to research customized, quartzy closure inside is ground, cover on bottleneck, bottleneck outer surface is also ground, it is easy to and quartzy bottle cap sealing, sterile breather plug socket is located at quartzy Bottle cap top, quartzy Bottle cap top has perforate under the sterile breather plug socket, is easy to plug after sterile plug and keeps filtrated air to enter in bottle.
Described inwall panel is quartzy panel.
Wave-length coverage is installed for 200-1000 nanometers of LEDs and LED daylight lamp on described LED light source plate, its medium wavelength range is the light source that 200-1000 nanometers of LEDs are used to adjust different wave length, LED daylight lamp is compareed for conventional light source, and the minimal gradient of the wavelength regulation of all-wave length LED matrix is 5-10 nanometers.
A kind of organism culturing device of Full-wavelength light source controllable, it is by peripheral casing, quartzy bottle, quartzy bottle cap, sterile breather plug socket, the three-dimensional LED illumination panel controller in the first face, and the second face solid LED illumination panel controller, the 3rd face is three-dimensional
LED illumination panel controller, fourth face solid LED illumination panel controller, the 5th face solid LED illumination panel controller, temperature, humidity and ultraviolet lamp controller, heater and compressor, humidifier, quartzy panel, LED lamps, gas valve, chamber door, boxboard composition.Its annexation is：Peripheral casing is that double-layer stainless steel panel is sandwiched between the heat preservation plate material that foam heat insulating material is made, and three-dimensional five surfaces of peripheral box inside are equipped with LED panels（The circuit board of used in electronic industry）, LED lamp beads are fixed in LED panel.Laterally or longitudinally to be arranged according to the wavelength order of lamp bead, i.e., LED lamp bead close-packed arrays on each circuit board, put in order：200nm, 205nm, 21 Onm 1000nm, 200nm, 205nm, the OOOnm of 21 Onm 1 and LED daylight lamp, the rest may be inferred, untill the end for being discharged to circuit board, the orientation of one of circuit board is opposite with the arrangement trend of the circuit board of opposite, to ensure fermented cells in casing, the uniformity of light source suffered by tissue or plant, the controller of five pieces of LED panels is located at the three-dimensional LED illumination panel controller in the first face below casing top respectively, second face solid LED illumination panel controller, 3rd face solid LED illumination panel controller, fourth face solid LED illumination panel controller, 5th face solid LED illumination panel controller,（As shown in Figure 1）, in addition, being connected positioned at temperature, humidity and ultraviolet lamp controller 6 below casing top with heater and mute compressor and humidifier.In the peripherally located casing of LED panel and case between quartz glass plate, the quartz glass plate all around with top in case is combined closely with bottom half stainless steel plate, forms space in a closed case.Top quartz glass plate, top LED panel and casing top panel have an aperture to be connected with gas valve, and bottom plate has the aperture of a formed objects, to ensure air circulation.
A kind of organism culturing device of Full-wavelength light source controllable, it includes peripheral casing, it is arranged on the LED luminescent screens in peripheral casing, the inwall being made up of in casing quartz glass plate, top, before, afterwards, the controller of the LED luminescent panels of left and right is respectively the three-dimensional LED illumination panel controller in the first face, second face solid LED illumination panel controller, 3rd face solid LED illuminating panel controllers, fourth face solid LED illumination panel controller, 5th face solid LED illumination panel controller, temperature, humidity and ultraviolet lamp controller are control appliance of temperature and humidity and bactericidal lamp shift knob, heater and compressor are to heat refrigeration system（Including a heater and a miniature silent compressor, and temperature sensor）, humidifier is humidifier.Heater and compressor and humidifier are digitized control by temperature, humidity and ultraviolet lamp controller, and gas valve is cross-ventilation hole, it is characterised in that：The face illumination of space five is provided, available for tissue cultures, cell culture, microculture, the culture of plant seedling and animal culture；Five face LED/light sources are respectively by controller control, and five controllers have wavelength selection and combination function and LED daylight lamp control function in 200-1000 nanometer ranges, and light application time program sets function；Cabinet wall is constituted by quartz glass panel, smooth sterile；Mute compressor ensures quiet in case；Humidity in humidifier regulating box, is easy to cultivate plant seedling.
Matching used with this incubator is quartzy blake bottle for tissue cultures or cell culture.Bottle is made up of quartz glass, is bottle cap to ensure the permeability of all wavelengths in 200-1000 nanometer ranges, is bottleneck, and the position that bottle cap is contacted with bottleneck contacts for frosted, is easy to sealing, is sterile breather plug insert port.It is characterized in that：Whole bottle is made up of quartz glass, right
Light wave in 200-1000 nanometer ranges has good permeability, it is adaptable to all-wave length bio-light repercussion study；Bottle cap solves tissue cultures or the ventilation problem of cell culture with frosted mouthful and breather plug socket, simplifies aseptic experiment operation.
The wave-length coverage of described all-wave length LED matrix is 200-1000 nanometers and LED daylight lamp, and the minimal gradient of the wavelength regulation of all-wave length LED matrix is 5-10 nanometers.
First face solid LED illumination panel controller, second face solid LED illumination panel controller, 3rd face solid LED illuminating panel controllers, fourth face solid LED illumination panel controller, the 5th face solid LED illumination panel controller is connected with top, front, rear, left and right LED panel respectively；The heating refrigerating plant and humidifier of compressor and heater including mute function are connected with controller and by controller control.
The wave-length coverage of described all-wave length LED matrix is 200-1000 nanometers, and the minimal gradient of the wavelength regulation of all-wave length LED matrix is 5-10 nanometers.Described LED lamp bead is fixed on circuit boards.Laterally or longitudinally to be arranged according to the wavelength order of lamp bead, i.e., LED lamp bead close-packed arrays on each circuit board, put in order：
200nm, 205nm, 21 Onm 1000nm, 200nm, 205nm, OOOnm the and LED fluorescent lamps of 21 Onm 1, the rest may be inferred, untill the end for being discharged to circuit board, and the orientation of one of circuit board is opposite with the arrangement trend of the circuit board of opposite, to ensure the uniformity of fermented cells in casing, light source suffered by tissue or plant seedling, the control function of each light source controller is：Multiple LED lamp beads parallel connection of Same Wavelength is used as a power supply unit in the LED panel that the controller is controlled.
Described light insulation pad will can be divided into four or multiple culture spaces, each culture space in casing can control the wavelength of light source respectively, can carry out the cell or tissue cultures contrast test of multiple wavelength light sources simultaneously in same incubator.
The inwall and light insulation pad being made including peripheral casing, the quartz glass being arranged in peripheral casing（As needed, light insulation pad can increase, reduces or remove), the LED panel for having five positionses in casing provides three-dimensional light source, heater, compressor and the humidifier for being arranged on bottom half and being connected with top controller.
The wave-length coverage of all-wave length LED matrix as described above is 200-1000 nanometers and LED daylight lamp, and the minimal gradient of the wavelength regulation of all-wave length LED devices is 5-10 nanometers.
The present apparatus, which also includes casing top gas valve, can keep having minute ventilation fan in aeration in case, gas valve.Compared with prior art, the beneficial effects of the invention are as follows：
1. it is simple in construction, it is simple to operate；
2. providing accurate light-source control system, it is adapted to scientific research needs.
3. Full-wavelength light source controllable is added on the basis of existing incubator, the blank of instrument and technology needed for having filled up bio-light repercussion study.
4. cabinet wall and culture bottle are quartz glass, it is ensured that the transmitance of each wavelength light source.
5. the need for according to fermented cells and purpose product, light source is made into the light source board of specific wavelength combination, can be used for work
Industry tissue cultures, the production for improving tissue cell growth speed, increment and purpose product using light reaction principle is illustrated
Fig. 1 is a kind of structural representation of the organism culturing device of Full-wavelength light source controllable.
Fig. 2 is a kind of quartzy Bottle structure schematic diagram.
Wherein：The faces of 1- first solid LED illumination panel controller, the faces of 2- second solid LED illumination panel controller, the faces of 3- the 3rd solid LED illumination panel controller, 4- fourth faces solid LED illumination panel controller, the faces of 5- the 5th solid LED illumination panel controller, 6- temperature, humidity and ultraviolet lamp controller, 12- casings, 13- blake bottles, 13a- quartz bottles, 13b- quartz bottle caps, the sterile breather plug sockets of 13c-, 13d- bottlenecks, 7a- heaters, 7b- compressors, 8- humidifiers, 9- inwalls panel (quartzy panel）, 10-LED light source boards, the valves of 11- first, the chamber doors of 14a- first, the chamber doors of 14b- second, the boxboards of 15a- first, the boxboards of 15b- second, the valves of 16- second.
It is specific real》Formula
Embodiment 1:
As shown in figure 1, a kind of organism culturing device of Full-wavelength light source controllable, including casing 12, blake bottle 13.First face solid LED illumination panel controller 1, second face solid LED illumination panel controller 2,3rd face solid LED illumination panel controller 3, fourth face solid LED illumination panel controller 4,5th face solid LED illumination panel controller 5, temperature, humidity and ultraviolet lamp controller 6 are separately fixed at cabinet wall, and its numerical monitor and parameter setting panel are fixed on the top outside casing.Heater 7a, compressor 7b, humidifier 8 these three parts are separately fixed on the bottom plate of casing 12, inwall panel（Quartzy panel）9 are covered on LED light source plate, and be separately fixed at by screw on the panel of casing 12, LED light source plate 10 is fixed on the corresponding panel of casing 12, gas valve 11 is fixed on above the top panel of casing 12, top panel has passage to be connected with the valve, the quartzy panel of case inner top and the LED panel of case inner top have a passage and connected with valve passage, for being ventilated in case.First chamber door 14a is fixed on the panel on the casing left side, and the second chamber door 14b is fixed on the panel on the right of the casing, and two chamber doors are split；First boxboard 15a is arranged on 1/2nd height in case, and the second boxboard 15b is arranged on the vertical center position of casing left and right directions.Blake bottle 13 is constituted：Quartzy bottle 13a height and diameter dimension can need customized according to research, it is ground inside quartzy bottle cap 13b, cover on bottleneck 13d, bottleneck 13d outer surfaces are also ground, it is easy to seal with bottle cap 13, sterile breather plug socket 13c is located at the top of quartzy bottle cap 13b, has perforate under the sterile breather plug socket 13c at the top of quartzy bottle cap 13b, is easy to plug after sterile plug and keeps filtrated air to enter in bottle.
Laterally or longitudinally to be arranged according to the wavelength order of lamp bead, i.e., LED light source plate close-packed arrays on each circuit board, put in order： 200nm,205nm,210nm 1000nm,200nm,205nm,21 Onm lOOOnm
And LED daylight lamp, the rest may be inferred, untill the end for being discharged to circuit board, the orientation of one of circuit board and opposite
Circuit board arrangement trend it is opposite, to ensure the uniformity of fermented cells in casing, light source suffered by tissue or plant, the controller of five pieces of LED panels is located at the three-dimensional LED illumination panel controller 1 in the first face below casing top respectively, second face solid LED illumination panel controller 2,3rd face solid LED illumination panel controller 3, fourth face solid LED illumination panel controller 4, the 5th face solid LED illumination panel controller 5 is (as shown in Figure 1）, in addition, temperature and appropriateness monitoring and control are located at temperature, humidity and ultraviolet lamp controller 6 below casing top, temperature, humidity and ultraviolet lamp controller 6 are connected with heater 7a, compressor 7b and humidifier 8.In the peripherally located casing 12 of LED light source plate 10 and case between quartz glass plate 9, the quartz glass plate all around with top in case is combined closely with bottom half stainless steel plate, forms space in a closed case.Top quartz glass plate has an aperture to be connected with gas valve 11, and bottom plate has the passage of a formed objects, to ensure air circulation.What the quartz glass being arranged in peripheral casing 12 was made（Quartzy panel）Inwall panel 9, LED light source plate 10 is provided between casing 12 and quartz wall panel 9, it is to provide three-dimensional light source in case from five faces, and respectively by the three-dimensional LED illumination panel controller 1 in the first face, second face solid LED illumination panel controller 2,3rd face solid LED illumination panel controller 3, fourth face solid LED illumination panel controller 4,5th face solid LED illumination panel controller 5 is controlled, the wave-length coverage of all-wave length LED matrix 10 is 200-1000 nanometers, and the minimal gradient of wavelength regulation is 5-10 nanometers.Also include being arranged on heater 7a, the compressor 7b of bottom half and be connected with the ultraviolet lamp controller 6 of casing top, the ultraviolet displays temperature of lamp controller 6 and design temperature are simultaneously automatically controlled.The humidifier 8 that is arranged in casing and it is connected with the ultraviolet lamp controller of casing top 6, display humidity and humidity is set, and is automatically controlled.The boxboard 15a and 15b separated for space in case.Blake bottle 13 in Fig. 2 is made for quartz glass.Bottle cap 13b, bottleneck 13d, sterile breather plug insert port 13c including bottle 13a, with ground, the all material of blake bottle 13 is made by quartz glass, to ensure the permeability of all wavelengths in 200-1000 nanometer ranges, bottle cap 13b is contacted with the bottleneck 13d positions contacted for frosted, is easy to sealing.
Embodiment 2:
A kind of Full-wavelength light source controllable organism culturing device laboratory applications method：
Biomaterial：Rhodiola rosea callus
Culture medium：The rhodiola rosea callus culture medium of optimization.
Light reaction condition research：
It is inoculated with after callus 0-24 hours：Dark culturing, cell adapted phase.
2-30 days after inoculation：620-630 nanometers of photo-irradiation treatments, surface intensity of illumination：400LUX (luxs）, it is the light wave condition that cell quickly divides growth, callus can be with rapid growth.30-60 days：(50% two kinds of light sources of expression respectively account for half formation mixing light source and irradiated simultaneously 420-440 nanometers of 50%+620-630 nanometers 50%, below similarly）, or 320-350 nanometers of 40%+620-630 nanometers 60%, or the 280-320 nanometers of photo-irradiation treatments of 10%+620-630 nanometers 90%, surface intensity of illumination：400LUX, is rhodiola root differentiation seedling and the optimization light wave condition taken root.It can repeatedly optimize and obtain rhodiola root callus group
Knit the optimal light wave combination that growth and tissue-cultured seedling break up.
Embodiment 3:
A kind of Full-wavelength light source controllable biological culture puts laboratory applications method：
Biomaterial：Cordyceps militaris i Cordyceps militaris t strains
Culture medium：Cordyceps militaris culture medium
Light-operated fermenting procedure：
0-48 hours after inoculation：Dark culturing, so that strain is adapted to and restoration ecosystem.
48-168 hours after inoculation：620-630 nanometers or 600-620 or 630-640 nanometers or 650-660 nanometers of photo-irradiation treatments, surface intensity of illumination：400LUX, repeatedly optimization can obtain the optimal light wave condition of mycelia division growth and adenosine biosynthesis.
168-288 hours after inoculation：380-400 nanometers or 400-410 nanometers or 410-420 nanometers or 420-440 nanometers of photo-irradiation treatments, surface intensity of illumination：400LUX, repeatedly optimization can obtain cordycepin and the optimal light wave condition of herxheimer-liked reaction.
Embodiment 4:
A kind of Full-wavelength light source controllable biological culture puts laboratory applications method：
Biomaterial：Ginseng separates cell
Culture medium：The ginseng-cell fluid nutrient medium of optimization
Light-operated culture program：
0-24 hours after inoculation：Dark culturing, so that cell adapted and restoration ecosystem.
24-168 hours after inoculation：620-630 nanometers of photo-irradiation treatments, surface intensity of illumination：400LUX, light wave condition is increased for the ginseng-cell division of optimization.Maximum ginseng-cell biomass can now be obtained.
168-288 hours after inoculation：620-630 nanometers carry out photo-irradiation treatment, surface intensity of illumination with the 420-440 nanometers of ratios for respectively accounting for 50%：400LUX, for the ginsenoside biosynthesis light wave condition of optimization.Maximum ginsenoside production can now be obtained
Claims (3)
- Claims1st, a kind of organism culturing device of Full-wavelength light source controllable, including casing（12), blake bottle（13), it is characterised in that：Casing（12) in：First face solid LED illumination panel controller（1), the three-dimensional LED illumination panel controller in the second face（2), the three-dimensional LED illumination panel controller in the 3rd face（3), the three-dimensional LED illumination panel controller of fourth face（4), the 5th face solid LED illuminating panel controllers（5), temperature, humidity and ultraviolet lamp controller（6) inner side of casing top panel is separately fixed at, is from left to right arranged in order；First face solid LED illumination panel controller（1) with the three-dimensional LED illumination panel controller in the second face（2) connect, the second face solid LED illumination panel controller（2) with the three-dimensional LED illumination panel controller in the 3rd face（3) connect, the 3rd face solid LED illumination panel controller（3) with the three-dimensional LED illumination panel controller of fourth face（4) connect, fourth face solid LED illumination panel controller（4) with the three-dimensional LED illumination panel controller in the 5th face（5) connect, the 5th face solid LED illuminating panel controllers（5) with temperature, humidity and ultraviolet lamp controller（6) connect；Each the numerical monitor and parameter setting panel of controller are exposed and are located at each self-corresponding position in top of chamber door；Heater（7a), compressor (7b), humidifier（8) three parts are separately fixed on box bottom；LED light source boards（10) it is fixed on corresponding casing（12) on panel inner wall；Inwall panel（9) LED light source boards are covered in（10) on；Other four sides inwalls are adopted in casing is set in a like fashion；First valve（11) it is fixed on casing（12) above top panel, the first valve（11) passage runs through casing in vertical position（12) top is in favor of ventilation；Second valve（16) it is positioned at casing（12) gas valve on bottom plate, the second valve（16) also casing is run through in vertical position（12) bottom, the first valve（11) with the second valve（16) it is used to ventilate in case；First chamber door（14a) it is fixed on the casing（12) on the panel on the left side, the second chamber door（14b) it is fixed on casing（12) on the panel on the right of, two chamber doors are split；First boxboard（15a) it is arranged on casing（12) 1/2nd height, the second boxboard in（15b) it is arranged on casing（12) the vertical center position of left and right directions in；Blake bottle（13) quartzy bottle is included（13a), quartzy bottle cap（13b), sterile breather plug socket（13c), quartzy bottle（13a) highly it is 5-20 centimetres, a diameter of 3-20 centimetres, quartzy bottle cap（13b) internal is ground, is covered in bottleneck（On 13d), bottleneck（13d) outer surface is also ground, is easy to and quartzy bottle cap（13b) seal, sterile breather plug socket（13c) it is located at quartzy bottle cap（13b) top, quartzy bottle cap（13b) top is in the sterile breather plug socket（There is perforate under 13c), be easy to plug after sterile plug and keep filtrated air to enter in bottle.2nd, the organism culturing device of a kind of Full-wavelength light source controllable according to claim 1, it is characterised in that：Described inwall Panel（9) it is quartzy panel.3rd, the organism culturing device of a kind of Full-wavelength light source controllable according to claim 1, it is characterised in that：Described LED light source boards（10) wave-length coverage is installed for 200-1000 nanometers of LEDs and LED daylight lamp on.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201280004480.8A CN103384720B (en) | 2011-12-16 | 2012-11-10 | A kind of organism culturing device of Full-wavelength light source controllable |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104244497 | 2011-12-16 | ||
| CN201110424449.7A CN102433259B (en) | 2011-12-16 | 2011-12-16 | Full-wavelength light source controllable biological culture device |
| CN201110424449.7 | 2011-12-16 | ||
| PCT/CN2012/084420 WO2013086915A1 (en) | 2011-12-16 | 2012-11-10 | Biological culturing device with complete wavelength light-control |
| CN201280004480.8A CN103384720B (en) | 2011-12-16 | 2012-11-10 | A kind of organism culturing device of Full-wavelength light source controllable |
Publications (2)
| Publication Number | Publication Date |
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| CN103384720A true CN103384720A (en) | 2013-11-06 |
| CN103384720B CN103384720B (en) | 2015-10-21 |
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| CN201110424449.7A Expired - Fee Related CN102433259B (en) | 2011-12-16 | 2011-12-16 | Full-wavelength light source controllable biological culture device |
| CN201280004480.8A Active CN103384720B (en) | 2011-12-16 | 2012-11-10 | A kind of organism culturing device of Full-wavelength light source controllable |
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| CN201110424449.7A Expired - Fee Related CN102433259B (en) | 2011-12-16 | 2011-12-16 | Full-wavelength light source controllable biological culture device |
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| WO (1) | WO2013086915A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102517210B (en) * | 2011-12-08 | 2014-05-14 | 湖北民族学院 | Biological reactor with full-wavelength controllable light sources |
| CN102433259B (en) * | 2011-12-16 | 2014-04-02 | 湖北民族学院 | Full-wavelength light source controllable biological culture device |
| CN203202817U (en) * | 2013-04-03 | 2013-09-18 | 李锋 | LED (Light-emitting Diode) biological lighting lamp |
| CN103270901B (en) * | 2013-05-17 | 2015-02-18 | 河北大学 | Habitable room flower multifunctional nursing system |
| CN103650928A (en) * | 2013-11-26 | 2014-03-26 | 大连好地农业有限公司 | Grafted seedling callusing system |
| CN103688853A (en) * | 2013-11-29 | 2014-04-02 | 广西壮族自治区农业科学院花卉研究所 | Tissue culture flask with light splitting function |
| CN103704063B (en) | 2013-12-17 | 2014-12-17 | 京东方科技集团股份有限公司 | Plant growing device and plant growing control method thereof |
| CN104542065A (en) * | 2014-12-25 | 2015-04-29 | 北京林业大学 | Plant incubator equipped with three-dimensional program-controlled LED (Light-Emitting Diode) illumination system |
| CN104962455A (en) * | 2015-06-24 | 2015-10-07 | 中山安荞生物科技有限公司 | Illumination bioreactor |
| CN106645667B (en) * | 2016-11-07 | 2018-12-25 | 中国农业科学院植物保护研究所 | It is a kind of for evaluating liquid fumigant to the method and device of earthworm acute toxicity |
| CN106645666B (en) * | 2016-11-07 | 2018-12-25 | 中国农业科学院植物保护研究所 | It is a kind of for evaluating solid fumigant to the method and device of earthworm acute toxicity |
| CN106645668B (en) * | 2016-11-07 | 2018-12-25 | 中国农业科学院植物保护研究所 | It is a kind of for evaluating liquid fumigant to the method and device of earthworm acute toxicity |
| CN106962025A (en) * | 2017-05-20 | 2017-07-21 | 天津市东方中滨农业科技有限公司 | A kind of method that utilization LED lamplight cultivates Cordyceps militaris |
| CN107937261A (en) * | 2017-11-16 | 2018-04-20 | 王孔旭成 | A kind of fresh and alive algae culturing device and cultural method |
| CN109187478A (en) * | 2018-10-09 | 2019-01-11 | 魏泽林 | A kind of automatic experimental provision of biological sample multimode |
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- 2011-12-16 CN CN201110424449.7A patent/CN102433259B/en not_active Expired - Fee Related
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2012
- 2012-11-10 WO PCT/CN2012/084420 patent/WO2013086915A1/en active Application Filing
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| CN101701183A (en) * | 2009-10-28 | 2010-05-05 | 福建师范大学 | Isolated cell photodynamic action effect reference apparatus based on LED array |
| CN101985599A (en) * | 2010-11-15 | 2011-03-16 | 中国科学院深圳先进技术研究院 | In-vitro light control cell co-culture device |
| CN102533522A (en) * | 2011-11-30 | 2012-07-04 | 镇江绿能环保科技有限公司 | Full-plastic airtight modularized airlift light biological reactor |
| CN102517210A (en) * | 2011-12-08 | 2012-06-27 | 湖北民族学院 | Biological reactor with full-wavelength controllable light sources |
| CN102433259A (en) * | 2011-12-16 | 2012-05-02 | 湖北民族学院 | Full-wavelength light source controllable biological culture device |
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| CN103384720B (en) | 2015-10-21 |
| WO2013086915A1 (en) | 2013-06-20 |
| CN102433259A (en) | 2012-05-02 |
| CN102433259B (en) | 2014-04-02 |
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