CN112823208A - 烟草转基因事件及用于检测与使用烟草转基因事件的方法 - Google Patents

烟草转基因事件及用于检测与使用烟草转基因事件的方法 Download PDF

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CN112823208A
CN112823208A CN201980055523.7A CN201980055523A CN112823208A CN 112823208 A CN112823208 A CN 112823208A CN 201980055523 A CN201980055523 A CN 201980055523A CN 112823208 A CN112823208 A CN 112823208A
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tobacco
event
plant
dna
sample
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奥代德·舒斯约夫
达芙娜·米切利
伊塔玛·卢波
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Collplant Ltd
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN201980055523.7A 2018-07-31 2019-07-31 烟草转基因事件及用于检测与使用烟草转基因事件的方法 Pending CN112823208A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862712289P 2018-07-31 2018-07-31
US62/712,289 2018-07-31
PCT/IL2019/050861 WO2020026241A1 (en) 2018-07-31 2019-07-31 Tobacco transgenic event and methods for detection and use thereof

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CN112823208A true CN112823208A (zh) 2021-05-18

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US (1) US20210310082A1 (https=)
EP (1) EP3827087A1 (https=)
JP (1) JP7479343B2 (https=)
KR (1) KR20210042430A (https=)
CN (1) CN112823208A (https=)
AU (1) AU2019315054A1 (https=)
BR (1) BR112021001586A2 (https=)
CA (1) CA3108243A1 (https=)
IL (1) IL280541A (https=)
WO (1) WO2020026241A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115572732A (zh) * 2022-01-25 2023-01-06 贵州省烟草科学研究院 一种培育栽培烟草单倍体诱导系的方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11935238B2 (en) * 2021-12-22 2024-03-19 RealizeMD Ltd. Systems and methods for generating clinically relevant images that preserve physical attributes of humans while protecting personal identity
US11631208B1 (en) * 2021-12-22 2023-04-18 RealizeMD Ltd. Systems and methods for generating clinically relevant images that preserve physical attributes of humans while protecting personal identity

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009128076A2 (en) * 2008-04-18 2009-10-22 Collplant Ltd. Methods of generating and using procollagen
CN105263511A (zh) * 2013-03-21 2016-01-20 科尔普兰特有限公司 用于组织再生的包含胶原和prp的组合物
CN106381327A (zh) * 2005-02-28 2017-02-08 二十二世纪有限公司 鉴定烟草植物的方法、生产烟草植物细胞的方法、植物细胞和降低了生物碱的产品
WO2017059795A1 (zh) * 2015-10-09 2017-04-13 上海交通大学 大豆转化事件shzd32-01及其应用方法

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3755560A (en) 1971-06-30 1973-08-28 Dow Chemical Co Nongreasy cosmetic lotions
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
DE2437090A1 (de) 1974-08-01 1976-02-19 Hoechst Ag Reinigungsmittel
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
US4421769A (en) 1981-09-29 1983-12-20 The Procter & Gamble Company Skin conditioning composition
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5073371A (en) 1990-11-30 1991-12-17 Richardson-Vicks, Inc. Leave-on facial emulsion compositions
US5073372A (en) 1990-11-30 1991-12-17 Richardson-Vicks, Inc. Leave-on facial emulsion compositions
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5667487A (en) 1993-04-07 1997-09-16 Henley; Julian L. Ionosonic drug delivery apparatus
US6007802A (en) 1996-03-27 1999-12-28 The Procter & Gamble Co. Conditioning shampoo composition
GB9804720D0 (en) 1998-03-05 1998-04-29 Unilever Plc Shampoo compositions
DE602005023332D1 (de) 2004-09-29 2010-10-14 Collplant Ltd Kollagenproduzierende pflanzen sowie verfahren zu deren erzeugung und verwendung
WO2009053985A1 (en) 2007-10-26 2009-04-30 Collplant Ltd. Methods of processing recombinant procollagen

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106381327A (zh) * 2005-02-28 2017-02-08 二十二世纪有限公司 鉴定烟草植物的方法、生产烟草植物细胞的方法、植物细胞和降低了生物碱的产品
WO2009128076A2 (en) * 2008-04-18 2009-10-22 Collplant Ltd. Methods of generating and using procollagen
CN105263511A (zh) * 2013-03-21 2016-01-20 科尔普兰特有限公司 用于组织再生的包含胶原和prp的组合物
WO2017059795A1 (zh) * 2015-10-09 2017-04-13 上海交通大学 大豆转化事件shzd32-01及其应用方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115572732A (zh) * 2022-01-25 2023-01-06 贵州省烟草科学研究院 一种培育栽培烟草单倍体诱导系的方法

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JP7479343B2 (ja) 2024-05-08
CA3108243A1 (en) 2020-02-06
AU2019315054A1 (en) 2021-03-18
KR20210042430A (ko) 2021-04-19
IL280541A (en) 2021-03-01
JP2021532780A (ja) 2021-12-02
EP3827087A1 (en) 2021-06-02
US20210310082A1 (en) 2021-10-07
WO2020026241A1 (en) 2020-02-06
BR112021001586A2 (pt) 2021-05-04

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