CN112795597A - Method for acclimatizing paddy field soil and promoting straw rapid hydrolysis - Google Patents
Method for acclimatizing paddy field soil and promoting straw rapid hydrolysis Download PDFInfo
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Abstract
The invention discloses a method for acclimating paddy soil to promote straw rapid hydrolysis, which acclimates paddy soil microorganisms through straw and fecal sewage substrates, induces the formation of groups of diverse straw decomposition compound bacteria, and effectively enhances the activity of soil enzymes, wherein the paddy soil plays the dual functions of a strain nest and an extracellular enzyme immobilization carrier secreted by the microorganisms. Then taking domesticated rice soil as a cheap inoculum, carrying out inoculation treatment on the straws, immersing the straws in liquid manure, keeping the straws in a micro-oxygen state, and further hydrolyzing the straws into smaller fragments or cellulose oligomers, hemicellulose oligomers and reducing sugar; stirring and primarily settling, separating liquid substances and fine organic matter particles in the straw manure mixture, transferring the liquid substances and the fine organic matter particles into an acidification tank containing abundant acetobacter and butyric acid bacillus, keeping low dissolved oxygen and high reaction temperature, and promoting the organic matter to be comprehensively and efficiently converted into acetic acid or butyric acid. Provides rich organic acid raw materials for methane production through continuous, stable and efficient anaerobic fermentation of the methane tank.
Description
Technical Field
The invention relates to a method for domesticating paddy field soil to promote straw to be hydrolyzed quickly, belongs to the field of agricultural resource and environmental science, and is a key applicable technical innovation for efficiently producing biogas, high-quality biogas residues, biogas slurry and organic fertilizer by straw-excrement combined fermentation.
Background
With the rapid development of intensive breeding industry, the environmental pollution problem caused by the rapid development of livestock and poultry breeding industry is increasingly serious, according to statistics of 2016 department of agriculture, livestock and poultry breeding waste generated in China every year reaches 38 hundred million tons, and the most effective method for recycling feces is anaerobic fermentation at present. But the problems of low carbon-nitrogen ratio, low gas production efficiency, serious ammonia inhibition and the like exist in the anaerobic fermentation process of the excrement, and the co-fermentation of the straw and the excrement is the best solution.
China is the largest producing country of straws, the annual output reaches more than 8 hundred million tons, but a large amount of straws are not reasonably utilized or disposed. Straw is burnt disorderly, so that energy and nutrients are wasted, and serious environmental pollution is caused. The method for producing the biogas by straw fermentation is the most effective way for obtaining biomass energy sources. However, most of the straws are lignocellulose with highly complex supermolecular structures and are compounded by three major components of cellulose, hemicellulose and lignin (see table 1). A large number of silicified protective cells also exist in part of the straws of the gramineous crops, so that the straws are difficult to be rapidly decomposed by microorganisms in a short time. To solve this problem, various methods of straw pretreatment have been used, including physical pretreatment, chemical pretreatment and microbial pretreatment. The chemical pretreatment methods have the problems of high cost and easy environmental pollution.
Table 1: lignocellulose content of wheat straw, corn straw and rice straw
The published Chinese invention patents: a method for improving the yield of straw anaerobic digestion biogas by sodium hydroxide solid-state normal temperature treatment (CN 1814762A) comprises the steps of fully and uniformly mixing a sodium hydroxide solution with a certain concentration with crushed straw, mixing the mixture to be in a wet state, and then placing the mixture in a container for sealed storage, wherein the method promotes the decomposition of lignin in the straw, but the lignin still generates great impact on the anaerobic fermentation tank for a long time after the mixture is directly put into the anaerobic fermentation tank, and the improvement of the efficiency of converting the straw into the biogas is limited;
the published Chinese invention patents: a method for improving methane yield of rice straw by wet soaking with pure ammonia at normal temperature (publication No. CN 102827879A) comprises placing chopped rice straw in a sealed bag, evacuating air in the bag, and mixing ammonia water and water uniformly and pouring into the sealed bag; the addition amount of the ammonia water is 2 to 6 percent of the dry weight of the straws measured by pure ammonia; the total mass of the water in the ammonia water and the water contained in the straws is 30-90% of the dry weight of the straws, the straws are stored in an environment with the temperature of 25-35 ℃, the pretreatment time is 90-110 h, and then the straws are added into an anaerobic fermentation reactor.
The published Chinese invention patents: a method for improving the anaerobic digestion gas production performance of straw stalks by combined pretreatment of calcium oxide and biogas slurry (publication No. CN 108384813A) comprises the steps of dissolving calcium oxide which is 6-12% of the dry weight of the stalks in a mixture of the stalks and the biogas slurry, standing for 1-4 days, and adding an inoculum of a pig manure anaerobic fermentation tank to perform mesophilic anaerobic digestion for 40-60 days. The method is not beneficial to the efficient utilization of lime, and simultaneously, the difference between the microorganisms in the anaerobic stage and the microorganisms in the straw hydrolysis acidification stage is ignored.
Compared with physical and chemical methods, the microbial pretreatment has the characteristics of mild conditions, low cost, no environmental pollution and the like. In the initial stage of microbial pretreatment research, most researchers isolated and screened lignocellulose-degrading bacteria by pure culture methods. However, complete degradation of lignocellulose is a result of the co-action of fungi, bacteria, actinomycetes and the corresponding microbial communities, so that a single species has some problems in maintaining the cellulolytic ability. In recent years, many studies have been made to treat lignocellulose with composite bacteria in order to achieve the purpose of rapid degradation, but so far, the actual rapid and effective biological methods applied to the pretreatment of biogas anaerobic fermentation are few.
Disclosure of Invention
The technical problem solved by the invention is as follows: aiming at the defects of the prior art, the method for acclimatizing the soil in the rice field to promote the rapid hydrolysis of the straws is provided, by acclimatizing the soil microorganisms in the rice field, the formation of diverse straw decomposition complex floras is induced and the soil enzyme activity is effectively enhanced, meanwhile, the rice soil plays the roles of a germ nest and a microorganism extracellular enzyme immobilization carrier, the activity of polyphenol oxidase is further enhanced by rich low-valence manganese elements in reductive rice soil, the decomposition of lignin is accelerated, the decomposition, transformation and resource utilization of the straws are effectively improved, and the method is low in cost, simple to operate, low in operating cost, clean and free of pollution.
In order to solve the technical problems, the invention adopts the following technical scheme:
And 2, taking 100 parts of air-dried straws, taking the rice soil domesticated in the step 1 as a cheap inoculum, inoculating 1-100% (in a water saturation state), carrying out inoculation treatment on the straws for 12-96h, keeping the dissolved oxygen amount in a water layer of 1-2cm at 6-8mg/L, fishing out, draining, transferring into a plastic tank, controlling the water evaporation speed, providing sufficient air, keeping the temperature of 20-40 ℃, fermenting for 24-96h under a dark condition, and disintegrating the complex structure of lignocellulose after the surfaces of the straws are completely colonized by microorganisms with rich diversity. If the conditions allow, spraying 80-120 parts of lime water to 100 parts of air-dried straws, wherein the lime water comprises quicklime (kg): water (L) ═ 1: and 8, then uniformly mixing, compacting, diffusing for 6-24 hours, and then inoculating with domesticated rice soil.
And 3, immersing 50-100 parts by weight of the structurally loosened straw obtained in the step 2 into a reaction vessel filled with 100-200 parts by weight of liquid manure, and continuously keeping the micro-oxygen (dissolved oxygen amount is 3-9mg/L) and light-shielding state to promote the straw to be further hydrolyzed into fragments below 0.1mm or into cellulose oligomer, hemicellulose oligomer, amino acid and reducing sugar.
And 4, stirring and primarily settling the container in the step 3, separating liquid substances in the straw manure mixture and fine organic matter particles dispersed in the straw manure mixture, transferring the liquid substances into an acidification tank containing abundant acetobacter and butyric acid bacteria, and keeping low dissolved oxygen (1-6mg/L) and reaction temperature of 40-60 ℃ to promote the organic matters to be comprehensively and efficiently converted into acetic acid or butyric acid. And the settled substances return to the soil acclimation pool of the paddy field to keep the diversity of the soil microorganisms of the paddy field and the strong soil enzyme activity.
And 5, carrying out anaerobic filtration on the liquid organic acid obtained in the step 4, and continuously and slowly inputting the liquid organic acid into a large-volume methane tank (the hydraulic retention time is based on 20-30 days as a design basis) for efficient and stable anaerobic fermentation to produce methane according to a dilution multiple of 1/16-20 under an anaerobic condition (the dissolved oxygen is close to zero). The filter residue is kept in the acidification tank as the source of the acidification strains.
Based on the fact that a large number of microorganisms capable of decomposing lignocellulose, including bacteria, fungi and actinomycetes, exist in the rice field soil and can be rapidly degraded in the rice field soil with a proper C/N ratio, strains rich in hydrolyzed straws are obtained by directionally inducing and domesticating the rice field soil by taking straw-excrement as a substrate and taking the carbon-nitrogen ratio of 25:1, wherein the strains comprise 25.7 percent of bacteroides (Bacteroidetes), 21.0 percent of Firmicutes (Firmicutes), 20.9 percent of Proteobacteria (Proteobacteria), 12.4 percent of chloroflexixi (chlorothalothrix) and the like, and the strain effect of the rice field soil as the microorganisms is utilized, and the effect of the synergistic action of multiple strains in the straw degradation is enhanced; the solid components of the soil are utilized to play the role of the microbial extracellular enzyme immobilization carrier, and the activity of cellulase, catalase and laccase in the domesticated rice soil is obviously improved through determination; meanwhile, the existence of manganese elements and copper ions in the strong reducing environment of the rice field soil plays an active role in improving the activity of catalase and laccase and promotes the degradation of straw lignin.
The invention relates to a key technology for realizing the methane production by straw-livestock pollution co-fermentation, which is characterized in that after straw and excrement are used as substrates to domesticate soil microorganisms in a rice field and used as cheap inocula to hydrolyze the straw, the microorganisms can quickly colonize on the surface of the straw (lime water pretreatment can be carried out on the straw with serious silicification when conditions are allowed), and extracellular enzyme is secreted to promote smaller fragments or cellulose oligomers, hemicellulose oligomers, reducing sugar and the like generated by straw disintegration so as to provide cheap organic acid raw materials for subsequent acidification and continuous and stable methane production system by continuous high-buffer three-stage anaerobic fermentation.
Compared with the prior art, the invention has the advantages that:
1. the method has the advantages of simplicity, convenience, innovativeness, practicability and scientificity, is compact and effective by using the domesticated rice field soil as the inoculum for straw hydrolysis, and is low in production cost, simple in operation, low in operation cost and free of pollution;
2. the method utilizes the straws and the excrement as substrates to carry out substrate induction domestication on the soil of the rice field, and induces the diversified straw decomposition composite flora to enrich by taking soil substances as a strain nest; the rice soil also plays a role of a microbial extracellular enzyme immobilization carrier, so that the activity of the soil enzyme is effectively enhanced; the activity of catalase and laccase for decomposing lignin is further enhanced by rich manganese elements and trace copper elements brought by feces in the rice soil with strong reducibility;
3. when the technology of the invention is adopted, the straws can not be pretreated, but if the conditions allow, a small amount of lime water can also be adopted to treat the straws with developed silicified cells, such as straws, to destroy the silicified cells and further reduce physical barriers.
Drawings
FIG. 1 is a schematic view showing the structure of the soil microbial community in the paddy field before and after the acclimation in example 1.
FIG. 2 is a schematic view showing the cellulase activity of the paddy soil before and after acclimation in example 1.
FIG. 3 is a schematic diagram showing the increase of Total Organic Carbon (TOC) produced by three domesticated straws after the inoculation treatment of the paddy soil.
FIG. 4 is a schematic view showing the improvement of the lignocellulose degradation effect of the rice straw after acclimation and the rice field soil inoculation treatment.
FIG. 5 is a schematic view showing the increase of the total amount of organic acids generated by acidification reaction of liquid products and fine organic particles generated by the inoculation treatment of the soil in the paddy field after three domesticated straws.
FIG. 6 is a schematic view showing that the total amount of methane produced by the methane production reaction of liquid organic acids produced by the domesticated three straws after the inoculation treatment of the paddy field soil is increased compared with the conventional one-step direct input.
Detailed Description
The invention will be further described with reference to specific embodiments and the accompanying drawings.
Example 1: a method for inducing and domesticating rice soil by using straws and pig manure as a cheap inoculum for promoting straw hydrolysis.
and 2, mixing 100 parts by weight of the mixture of the straws and the pig manure in the step 1 with 1000 parts by weight of the paddy soil, keeping a 2cm submerged layer, naturally drying after 2 days, carrying out secondary rehydration after the water content of the soil surface layer is reduced to 60%, domesticating the soil microorganisms in the paddy soil for 4 times, keeping the 2cm submerged layer after the domestication is finished, and keeping the dissolved oxygen of the water layer at 6-8 mg/L. Inducing the formation of diversified straw decomposition compound floras and effectively enhancing the activity of soil enzymes, wherein the paddy soil plays the roles of a germ nest and a microorganism extracellular enzyme immobilization carrier, and due to the reducibility formed by the decomposition of organic matters, the low-valence manganese elements in the paddy soil are increased, and the trace copper ions brought into the soil by the excrement are generated;
and 4, collecting the domesticated paddy field soil to determine the soil cellulase activity, extracting DNA for high-throughput sequencing, and showing the results in the figure 1 and the figure 2, wherein the soil cellulase activity and the microbial community structure change.
As can be seen from FIG. 1, the distribution of Firmicutes, Chloroflexi, Proteobactera and Bacteroides in the soil microorganisms of paddy field acclimatized by straw and manure was more uniform than that of the control example. Firmicutes, Chloroflexi, Proteobacteria and bacterioides are considered to be the main phylum responsible for hydrolysis, producing extracellular enzymes to metabolize the substrate, break down cellulose and polysaccharides in the substrate and participate efficiently in the hydrolysis stage. Oceanirhabdus and Terrisporabacter in Firmicutes play an important role in the biodegradation of organic matter during the hydrolysis stage. Metagenome in Chroflexi plays a useful role in the degradation of butyrate and refractory organics in substrates. Simplicispira and Aquamibium in Proteobacteria are facultative and obligate heterotrophic bacteria which can reduce sulfate, degrade propionate, butyrate and monosaccharide; and utilizes long chain fatty acids and amino acids. Lentimicrobiaceae in Bacteroides is an important organic heterotrophic bacterium involved in the recovery of organic carbon and protein materials. Under the synergistic action of Firmicutes, Chloroflexi, Proteobacteria and Bacteroides, the lignin of the straws can be effectively degraded by destroying the straws, and the availability of the straws for being subjected to the enzymolysis of microbial exoenzymes is improved.
As can be seen from fig. 2, the cellulase activity of the acclimatized paddy soil was increased by 3.6 times as compared with the control example. The rice soil plays the role of a microbial extracellular enzyme immobilized carrier, and lays a foundation for the subsequent hydrolysis of cellulose in the straws.
Example 2: the acclimated paddy soil in the example 1 is used as an inoculum to promote the rapid hydrolysis of the straws after the pretreatment of the lime water.
and 4, immersing 50 parts by weight of the straw with the loosened structure obtained in the step 3 into a reaction vessel filled with 200 parts by weight of liquid manure, and continuously maintaining the state of micro oxygen (dissolved oxygen amount is 4mg/L) and keeping away from light to promote the straw to be further hydrolyzed into fragments with the size of less than 0.1mm or into cellulose oligomer, hemicellulose oligomer, amino acid and reducing sugar.
And 5, stirring and primarily settling the container in the step 4, separating liquid substances in the straw manure mixture and fine organic matter particles dispersed in the liquid substances, transferring the liquid substances into an acidification tank containing abundant acetobacter and butyric acid bacilli, and keeping low dissolved oxygen (4mg/L) and reaction temperature of 50 ℃ to promote the organic matters to be comprehensively and efficiently converted into acetic acid or butyric acid. The settled substances return to the soil domestication pool of the paddy field to keep the diversity of the domesticated soil microorganisms and the strong soil enzyme activity;
and 6, carrying out anaerobic filtration on the liquid organic acid obtained in the step 5, and continuously and slowly inputting the liquid organic acid into a large-volume methane tank (the hydraulic retention time is based on 25 days) for high-efficiency stable anaerobic fermentation to produce methane according to the dilution multiple of 1/20 under the anaerobic condition (the dissolved oxygen is close to zero). The filter residue equivalent to one tenth of the volume of the acidification tank is kept in the acidification tank as the source of the acidification strains.
Example 3: the acclimatized paddy soil in the example 1 is used as an inoculum to promote the quick hydrolysis of sorghum straws.
and 3, immersing 100 parts by weight of the structurally loosened sorghum straws obtained in the step 2 into a reaction container filled with 200 parts by weight of liquid manure, continuously keeping a micro-oxygen (dissolved oxygen amount is 5mg/L) and light-shielding state, and promoting the sorghum straws to be further hydrolyzed into fragments with the diameter of less than 0.1mm or into cellulose oligomers, hemicellulose oligomers, amino acids and reducing sugars.
And 4, stirring and primarily settling the container in the step 3, separating liquid matters in the sorghum straw manure mixture and fine organic matter particles dispersed in the sorghum straw manure mixture, transferring the sorghum straw manure mixture into an acidification tank containing abundant acetobacter and butyric acid bacteria, and keeping low dissolved oxygen (2.5mg/L) and reaction temperature of 55 ℃ to promote the organic matters to be comprehensively and efficiently converted into acetic acid or butyric acid. The settled material is returned to the rice field to maintain the diversity and strong soil enzyme activity of the domesticated rice field soil microorganisms;
and 5, carrying out anaerobic filtration on the liquid organic acid obtained in the step 4, and continuously and slowly inputting the liquid organic acid into a large-volume methane tank (the hydraulic retention time is based on 25 days as a design basis) for high-efficiency stable anaerobic fermentation to produce methane according to the dilution multiple of 1/18 under the anaerobic condition (the dissolved oxygen is close to zero). The filter residue equivalent to one tenth of the volume of the acidification tank is kept in the acidification tank as the source of the acidification strains.
Example 4: a method for inducing and domesticating rice soil by using wheat straws and chicken manure as a cheap inoculum for promoting hydrolysis of the wheat straws.
and 2, mixing 100 parts by weight of the mixture of the wheat straws and the chicken manure in the step 1 with 2000 parts by weight of the paddy soil, keeping a 1.8cm flooded layer, naturally drying after 2 days, repeating for 4 times when the water content of the surface layer of the soil is reduced to 70%, domesticating the soil microorganisms in the paddy soil, keeping a 1.5cm flooded layer after the domestication is finished, and keeping the dissolved oxygen amount of the water layer to be 6.5 mg/L. Inducing the formation of diversified wheat straw decomposition compound floras and effectively enhancing the activity of soil enzymes, wherein the rice soil plays the roles of a germ nest and a microorganism extracellular enzyme immobilization carrier, and due to the reducibility formed by the decomposition of organic matters, the low-valent manganese elements in the rice soil are increased, and the trace copper ions brought into the soil by the manure are generated;
example 5: the domesticated paddy soil of the embodiment 4 is used as an inoculum to promote the rapid hydrolysis of the wheat straws.
and 3, immersing 100 parts by weight of the wheat straw with the loosened structure obtained in the step 2 into a reaction container filled with 200 parts by weight of liquid chicken manure, and continuously keeping the micro-oxygen (dissolved oxygen amount is 3.8mg/L) and light-proof state to promote the wheat straw to be further hydrolyzed into fragments with the diameter of less than 0.1mm or into cellulose oligomer, hemicellulose oligomer, amino acid and reducing sugar.
And 4, stirring and primarily settling the container in the step 3, separating liquid matters in the wheat straw manure mixture and fine organic matter particles dispersed in the mixture, transferring the mixture into an acidification tank containing abundant acetobacter and butyric acid bacteria, and keeping low dissolved oxygen (3mg/L) and reaction temperature of 52 ℃ to promote the organic matters to be comprehensively and efficiently converted into acetic acid or butyric acid. The settled substances return to the soil domestication pool of the paddy field to keep the diversity of the domesticated soil microorganisms and the strong soil enzyme activity;
and 5, carrying out anaerobic filtration on the liquid organic acid obtained in the step 4, and continuously and slowly inputting the liquid organic acid into a large-volume methane tank (the hydraulic retention time is based on 25 days as a design basis) for high-efficiency stable anaerobic fermentation to produce methane according to the dilution multiple of 1/19 under the anaerobic condition (the dissolved oxygen is close to zero). The filter residue equivalent to one tenth of the volume of the acidification tank is kept in the acidification tank as the source of the acidification strains.
Example 6: a method for inducing and domesticating rice soil by using corn stalks and cow dung as a cheap inoculum for promoting the hydrolysis of the corn stalks.
and 2, mixing 100 parts by weight of the mixture of the corn straws and the dairy cow dung in the step 1 with 3000 parts by weight of the paddy soil, keeping a 2cm submerged layer, naturally drying after 2 days, carrying out secondary rehydration when the water content of the surface layer of the soil is reduced to 65%, domesticating the soil microorganisms in the paddy soil for 4 times, keeping the 2cm submerged layer after the domestication is finished, and keeping the dissolved oxygen content of the water layer to be 8 mg/L. Inducing the formation of the diversified maize straw decomposition compound flora and effectively enhancing the activity of the soil enzyme, wherein the paddy soil plays the roles of a strain nest and a microorganism extracellular enzyme immobilization carrier, and due to the reducibility formed by the decomposition of organic matters, the low-valence manganese elements in the paddy soil are increased, and the trace copper ions brought into the soil by the excrement are generated;
example 7: the acclimatized paddy soil in the example 6 is used as an inoculum to promote the rapid hydrolysis of the corn straws.
and 3, immersing 100 parts by weight of the structurally loosened corn straws obtained in the step 2 into a reaction container filled with 200 parts by weight of liquid dairy cow dung, and continuously keeping the micro-oxygen (dissolved oxygen amount is 3.8mg/L) and light-shielding state to promote the corn straws to be further hydrolyzed into fragments with the diameter of less than 0.1mm or into cellulose oligomers, hemicellulose oligomers, amino acids and reducing sugars.
And 4, stirring and primarily settling the container in the step 3, separating liquid substances in the corn straw and cow dung mixture and fine organic matter particles dispersed in the liquid substances, transferring the liquid substances into an acidification tank containing abundant acetobacter and butyric acid bacteria, keeping low dissolved oxygen (3mg/L) and reaction temperature of 52 ℃, and promoting the organic matters to be comprehensively and efficiently converted into acetic acid or butyric acid. The settled substances return to the soil domestication pool of the paddy field to keep the diversity of the domesticated soil microorganisms and the strong soil enzyme activity;
and 5, carrying out anaerobic filtration on the liquid organic acid obtained in the step 4, and continuously and slowly inputting the liquid organic acid into a large-volume methane tank (the hydraulic retention time is based on 25 days as a design basis) for high-efficiency stable anaerobic fermentation to produce methane according to the dilution multiple of 1/19 under the anaerobic condition (the dissolved oxygen is close to zero). The filter residue equivalent to one tenth of the volume of the acidification tank is kept in the acidification tank as the source of the acidification strains.
The foregoing merely represents preferred embodiments of the invention, which are described in some detail and detail, and therefore should not be construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, various changes, modifications and substitutions can be made without departing from the spirit of the present invention, and these are all within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (6)
1. A method for acclimatizing paddy field soil to promote straw rapid hydrolysis is characterized by comprising the following steps:
step 1, mixing 100-500 parts by weight of straw and excrement with 1000-5000 parts by weight of paddy soil, keeping a 1-2cm submerged layer, naturally drying after 1-2 days, repeating for 3-4 times when the water content of the surface layer of the soil is reduced to 50% -60%, domesticating soil microorganisms of the paddy field, and repeatedly adding straw and excrement substrates once every two months, wherein the quantity is one tenth of the quantity of the first addition;
step 2, taking 100 parts of air-dried straws, taking the rice soil domesticated in the step 1 as an inoculum, wherein the inoculum size is 1% -100%, carrying out inoculation treatment on the straws for 12-96h, keeping the dissolved oxygen amount in a water layer of 1-2cm at 6-8mg/L, fishing out, draining, transferring into a plastic tank, controlling the water evaporation speed, providing sufficient air, keeping the temperature at 20-40 ℃, and fermenting for 24-96h under a light-tight condition;
step 3, immersing 50-100 parts by weight of the structurally loosened straw obtained in the step 2 into a reaction vessel filled with 100-200 parts by weight of liquid manure, continuously keeping the micro-oxygen and light-proof state, and hydrolyzing the straw into fragments with the diameter of less than 0.1mm or into cellulose oligomer, hemicellulose oligomer, amino acid and reducing sugar;
step 4, stirring and primarily settling the container in the step 3, separating out liquid matters in the straw manure mixture and fine organic matter particles dispersed in the straw manure mixture, transferring the liquid matters into an acidification tank containing abundant acetobacter and butyric acid bacteria, keeping low dissolved oxygen and reaction temperature of 40-60 ℃, converting the organic matters into acetic acid or butyric acid, and returning settled substances to a paddy field soil acclimation pool;
and 5, carrying out anaerobic filtration on the liquid organic acid obtained in the step 4, continuously and slowly inputting the liquid organic acid into a large-volume methane tank under an anaerobic condition according to a dilution multiple of more than 1/16-20 to carry out efficient and stable anaerobic fermentation to produce methane, and keeping filter residues in an acidification tank as a source of acidification strains.
2. The method for acclimatizing paddy soil to promote straw rapid hydrolysis according to claim 1, wherein the carbon-nitrogen ratio of straw + fecal sewage is 25: 1.
3. The method for acclimatizing paddy field soil to promote straw rapid hydrolysis according to claim 1, wherein the air-dried straw of step 2 is sprayed with 80-120 parts of lime water to 100 parts of air-dried straw before inoculation, wherein the lime water comprises quicklime (kg): water (L) ═ 1: and 8, then uniformly mixing, compacting, diffusing for 6-24 hours, and then inoculating with domesticated rice soil.
4. The method for acclimatizing paddy soil to promote straw rapid hydrolysis according to claim 1, wherein the dissolved oxygen amount of the micro-oxygen is 3-9 mg/L.
5. The method for acclimatizing paddy soil to promote rapid hydrolysis of straws as claimed in claim 1, wherein the low dissolved oxygen amount is 1-6 mg/L.
6. The method for acclimatizing paddy soil to promote rapid hydrolysis of straws as claimed in claim 1, wherein the hydraulic retention time in the step 5 is 20-30 days.
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| CN116144714A (en) * | 2023-03-06 | 2023-05-23 | 哈尔滨工业大学 | Method for preparing caproic acid by means of mixed flora electric domestication and straw cathodic electric fermentation reinforcement |
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