CN112795512A - Microbial preparation for treating potato starch wastewater and application method thereof - Google Patents
Microbial preparation for treating potato starch wastewater and application method thereof Download PDFInfo
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- 239000002351 wastewater Substances 0.000 title claims abstract description 80
- 230000000813 microbial effect Effects 0.000 title claims abstract description 75
- 238000002360 preparation method Methods 0.000 title claims abstract description 63
- 229920001592 potato starch Polymers 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 16
- 241000209140 Triticum Species 0.000 claims abstract description 34
- 235000021307 Triticum Nutrition 0.000 claims abstract description 33
- 244000005700 microbiome Species 0.000 claims abstract description 26
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000004202 carbamide Substances 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 18
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 150000001875 compounds Chemical class 0.000 claims abstract description 14
- 238000011010 flushing procedure Methods 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims description 31
- 241000132177 Aspergillus glaucus Species 0.000 claims description 29
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 28
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 19
- 241000190984 Rhodospirillum rubrum Species 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 230000000243 photosynthetic effect Effects 0.000 claims description 12
- 241000191025 Rhodobacter Species 0.000 claims description 5
- 238000009472 formulation Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000002994 raw material Substances 0.000 abstract description 5
- 239000000843 powder Substances 0.000 description 87
- 230000001580 bacterial effect Effects 0.000 description 45
- 239000007788 liquid Substances 0.000 description 27
- 239000007787 solid Substances 0.000 description 21
- 238000012360 testing method Methods 0.000 description 14
- 239000002131 composite material Substances 0.000 description 11
- 239000001963 growth medium Substances 0.000 description 10
- 241000191023 Rhodobacter capsulatus Species 0.000 description 9
- 235000013339 cereals Nutrition 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 description 5
- 238000013329 compounding Methods 0.000 description 5
- 238000011049 filling Methods 0.000 description 5
- 238000009630 liquid culture Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 210000005069 ears Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000012015 potatoes Nutrition 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000013573 potato product Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G25/00—Watering gardens, fields, sports grounds or the like
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
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Abstract
The invention discloses a microbial preparation for treating potato starch wastewater and an application method thereof. The microbial preparation is prepared by mixing compound microbes and urea. The two raw material components are 40-50 parts of compound microorganism and 50-60 parts of urea according to weight. The application process of the microbial preparation comprises the following steps: step 1, adding a microbial preparation into potato starch wastewater according to a set proportion; 2, performing facultative fermentation on the wastewater treated in the step 1 at the temperature higher than 4 ℃; and 3, flushing the wastewater treated in the step 2 into a wheat field. The microbial preparation provided by the invention is used for treating potato starch wastewater, can reduce the pollution of the wastewater to the environment, and effectively utilizes organic matters in the wastewater. The treated wastewater is used for planting wheat, so that the growth of wheat can be promoted, and the yield of wheat can be increased.
Description
Technical Field
The invention belongs to the technical field of wastewater treatment, and particularly relates to a microbial preparation for treating potato starch wastewater and an application method thereof.
Background
With the continuous and deep knowledge of the nutritional value of potatoes, people have increasingly demanded potato products, resulting in the increasing scale of potato processing.
The potato starch wastewater is waste liquid generated in the production process of producing starch by taking potatoes as raw materials, wherein the COD content can reach more than 10000mg/L, so the potato starch wastewater is highly polluted wastewater. But from one perspective, the waste water is also an abundant organic resource. The direct discharge of the potato starch wastewater causes serious environmental pollution and huge resource waste.
Therefore, the development of a microbial preparation containing beneficial microorganisms and the application of the microbial preparation to the treatment of potato starch wastewater have great significance to the potato processing industry.
Disclosure of Invention
The invention aims to provide a microbial preparation for treating potato starch wastewater, which is applied to the treatment of wastewater from potato production and processing, reduces the pollution of the wastewater to the environment and effectively utilizes organic matters in the wastewater.
In one aspect, the invention provides a microbial preparation for treating potato starch wastewater, which is prepared by mixing compound microorganisms and urea.
Preferably, the microbial preparation is prepared by mixing 40-50 parts of the compound microorganism and 50-60 parts of urea by weight.
Preferably, the microbial preparation is prepared by mixing 45-50 parts of the compound microorganisms and 50-55 parts of urea by weight.
Preferably, the microbial preparation is prepared by mixing 47 parts of the compound microorganisms and 53 parts of urea by weight.
Preferably, the complex microorganism includes aspergillus glaucus, photosynthetic bacteria and bacillus amyloliquefaciens.
Preferably, the photosynthetic bacteria are at least one of rhodopseudomonas palustris, rhodospirillum rubrum or rhodobacter capsulatum.
In another aspect, the present invention also provides a method for using the microbial preparation, which comprises the following steps:
step 1, adding a microbial preparation into potato starch wastewater according to a set proportion;
2, performing facultative fermentation on the wastewater treated in the step 1 at the temperature higher than 4 ℃;
and 3, flushing the wastewater treated in the step 2 into a wheat field.
Preferably, the weight ratio of the microbial agent to the potato starch wastewater in the step 1 is 1-5: 100.
Preferably, the fermentation time in step 2 is 30-180 days.
Preferably, the flushing amount of the wastewater in the step 3 is 15-75t/hm every time2。
The microbial preparation provided by the invention is used for treating potato starch wastewater, so that the pollution of the wastewater to the environment can be reduced, and organic matters in the wastewater can be effectively utilized. The treated wastewater is used for planting wheat, so that the growth of wheat can be promoted, and the yield of wheat can be increased.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
According to the invention, the composite microorganism and urea are mixed according to a certain proportion to obtain the microbial preparation for treating the potato starch wastewater. The microbial preparation provided by the invention is used for treating potato starch wastewater, can reduce the pollution of the wastewater to the environment, and effectively utilizes organic matters in the wastewater. The treated wastewater is used for planting wheat, so that the growth of wheat can be promoted, and the yield of wheat can be increased.
In one embodiment of the invention, the microbial preparation for treating the potato starch wastewater is prepared by mixing the compound microorganisms and urea. The specific preparation process comprises the following steps:
1) selecting Aspergillus glaucus powder, photosynthetic bacteria powder and Bacillus amyloliquefaciens powder, and then compounding the three kinds of bacteria powder according to the proportioning relationship by weight to obtain the composite microorganism, wherein 5-15 parts of Aspergillus glaucus powder, 15-30 parts of photosynthetic bacteria powder and 10-20 parts of Bacillus amyloliquefaciens powder are preferred. Wherein the photosynthetic bacteria is preferably at least one of Rhodopseudomonas palustris (Rhodopseudomonas palustris), Rhodospirillum rubrum (Rhodospirillum rubrum) or Rhodobacter capsulatus (Rhodobacter capsulatus). The five bacterial powders can be prepared by self, on one hand, rhodopseudomonas palustris, rhodospirillum rubrum, rhodobacter capsulatum and bacillus amyloliquefaciens are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacteria in the bacterial liquids are separated, concentrated and dried to obtain single bacterial strain powder. The mesh number of the four single-strain bacterial powders is preferably more than or equal to 100 meshes, and the bacterial content of each single-strain bacterial powder is as follows: the bacteria content of Rhodopseudomonas palustris is not less than 5.0 × 1010cfu/g, the bacterium content of Rhodospirillum rubrum is not less than 4.0 × 1010cfu/g, the bacterium content of rhodobacter capsulatus is more than or equal to 4.5 multiplied by 1010cfu/g, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 2.0 multiplied by 1010cfu/g. On the other hand, the aspergillus glaucus is prepared into liquid seed liquid firstly, then the liquid seed liquid is transferred into a corresponding solid culture medium, a solid culture is obtained through fermentation, then the solid culture is dried and crushed to prepare single-strain solid bacterial powder, the mesh number of the aspergillus glaucus solid bacterial powder is preferably more than or equal to 100 meshes, and the bacterial content of the aspergillus glaucus is more than or equal to 3.0 multiplied by 109cfu/g. In addition, the five bacterial powders can also adopt the qualified products sold in the market.
2) Weighing the compound microorganism and urea according to a predetermined weight ratio, adding the two raw materials together, stirring, and uniformly mixing to obtain the microbial preparation. The weight ratio of the two raw material components is preferably 40-50 parts of composite microorganism and 50-60 parts of urea.
When the microbial preparation is applied, the microbial preparation is added into the potato starch wastewater according to a set proportion. Wherein the weight ratio of the microbial agent to the potato starch wastewater is preferably 1-5: 100. Then, the wastewater added with the microbial preparation is subjected to facultative fermentation at a temperature higher than 4 ℃, and the fermentation time is preferably 30-180 days. Then the wastewater after fermentation treatment is flushed into the wheat field, wherein the flushing amount of the wastewater is preferably 15-75t/hm every time2。
To help better understand the technical solution of the present invention, the following examples are provided to illustrate the preparation process and application method of the microbial preparation for treating potato starch wastewater of the present invention.
Example one
The microbial preparation of the present example was prepared by mixing 40 parts of composite microorganisms and 60 parts of urea by weight. The composite microorganism is prepared by compounding aspergillus glaucus powder, rhodopseudomonas palustris powder and bacillus amyloliquefaciens powder, wherein the mesh number of the three kinds of the powder is 100 meshes of the aspergillus glaucus powder, 110 meshes of the rhodopseudomonas palustris powder and 115 meshes of the bacillus amyloliquefaciens powder. The three bacteria powder are respectively 5 parts of aspergillus glaucus powder, 22 parts of rhodopseudomonas palustris powder and 20 parts of bacillus amyloliquefaciens powder by weight.
The inventor prepares the three bacterial powders, on one hand, the rhodopseudomonas palustris and the bacillus amyloliquefaciens are fermented and cultured in corresponding liquid culture media respectively to obtain bacterial liquids, and then the bacteria in the bacterial liquids are separated, concentrated and dried to obtain single bacterial strain powder. The bacterial contents of the two single bacterial strains are respectively as follows: the bacteria content of Rhodopseudomonas palustris is not less than 5.0 × 1010cfu/g, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 2.0 multiplied by 1010cfu/g. On the other hand, the aspergillus glaucus is prepared into liquid seed liquid firstly, then is transferred into a corresponding solid culture medium, is fermented to obtain a solid culture, and then the solid culture is driedDrying and pulverizing to obtain single-strain solid powder containing Aspergillus glaucus of not less than 3.0 × 109cfu/g。
The preparation process of the microbial preparation comprises the following steps:
1) preparing the composite microorganism, and uniformly mixing the aspergillus glaucus powder, the rhodopseudomonas palustris powder and the bacillus amyloliquefaciens powder to obtain the composite microorganism.
2) Weighing Aspergillus glaucus powder, Rhodopseudomonas palustris powder, Bacillus amyloliquefaciens powder and urea according to a preset weight ratio, adding the raw materials together, stirring, and mixing uniformly to obtain the microbial preparation 1.
The application method of the microbial preparation 1 comprises the following steps: according to the proportion of adding 1kg of microbial preparation to every 100kg of potato starch wastewater, firstly adding the microbial preparation 1 into the potato starch wastewater, and then carrying out facultative fermentation on the wastewater added with the microbial preparation for 30 days at the temperature higher than 4 ℃. Then respectively flushing the fermented wastewater into the wheat field in the jointing stage, the booting stage and the filling stage of the wheat, wherein the flushing amount of the wastewater is 15t/hm every time2。
Example two
The microbial preparation of the embodiment is prepared by mixing 45 parts of compound microorganisms and 55 parts of urea by weight. The composite microorganism is prepared by compounding aspergillus glaucus powder, rhodospirillum rubrum powder and bacillus amyloliquefaciens powder, wherein the mesh number of the three kinds of the powder is 110 meshes of the aspergillus glaucus powder, 100 meshes of the rhodospirillum rubrum powder and 120 meshes of the bacillus amyloliquefaciens powder. The three bacteria powder are 8 parts of aspergillus glaucus powder, 26 parts of rhodospirillum rubrum powder and 10 parts of bacillus amyloliquefaciens powder respectively by weight.
The inventor prepares the three bacterial powders, on one hand, the rhodospirillum rubrum and the bacillus amyloliquefaciens are fermented and cultured in corresponding liquid culture media respectively to obtain bacterial liquids, and then the bacteria in the bacterial liquids are separated, concentrated and dried to obtain single bacterial strain bacterial powders. The bacterial contents of the two single bacterial strains are respectively as follows: the content of Rhodospirillum rubrum is not less than 4.0 × 1010cfu/g, solutionThe bacteria content of the bacillus amyloliquefaciens is more than or equal to 3.0 multiplied by 1010cfu/g. On the other hand, the aspergillus glaucus is prepared into liquid seed liquid, then the liquid seed liquid is transferred into a corresponding solid culture medium, a solid culture is obtained by fermentation, then the solid culture is dried and crushed to prepare single-strain solid bacterial powder, wherein the bacterial content of the aspergillus glaucus is more than or equal to 3.5 multiplied by 109cfu/g。
A microbial preparation 2 was prepared in the same manner as in example 1, except that Rhodospirillum rubrum powder was replaced with Rhodopseudomonas palustris powder.
The application method of the microbial preparation 2 comprises the following steps: according to the proportion of adding 3kg of microbial agent to every 100kg of potato starch wastewater, firstly adding the microbial agent 2 into the potato starch wastewater, and then carrying out facultative fermentation on the wastewater added with the microbial agent for 90 days at the temperature higher than 4 ℃. Then respectively flushing the fermented wastewater into the wheat field in the jointing stage, the booting stage and the filling stage of the wheat, wherein the flushing amount of the wastewater is 30t/hm every time2。
EXAMPLE III
The microbial preparation of the present example was prepared by mixing 47 parts by weight of composite microorganisms and 53 parts by weight of urea. The composite microorganism is prepared by compounding aspergillus glaucus powder, rhodobacter capsulatus powder and bacillus amyloliquefaciens powder, wherein the mesh number of the three kinds of the powder is 120 meshes of the aspergillus glaucus powder, 115 meshes of the rhodospirillum rubrum powder and 100 meshes of the bacillus amyloliquefaciens powder. The three bacteria powder are respectively 13 parts of aspergillus glaucus powder, 30 parts of rhodobacter capsulatus powder and 14 parts of bacillus amyloliquefaciens powder by weight.
The inventor prepares the three bacterial powders, on one hand, firstly, rhodobacter capsulatus and bacillus amyloliquefaciens are respectively fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then, thalli in the bacterial liquids are separated, concentrated and dried to obtain single bacterial strain powder. The bacterial contents of the two single bacterial strains are respectively as follows: the bacteria content of rhodobacter capsulatus is not less than 4.5 × 1010cfu/g, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 2.5 multiplied by 1010cfu/g. On the other hand, the Aspergillus glaucus is prepared into liquid seedsInoculating the liquid into solid culture medium, fermenting to obtain solid culture, drying the solid culture, and pulverizing to obtain single-strain solid powder with Aspergillus glaucus content of 4.0 × 109cfu/g。
Microbial preparation 3 was prepared in the same manner as in example 1, except that rhodobacter capsulatus powder was replaced with rhodopseudomonas palustris powder.
The application method of the microbial preparation 3 comprises the following steps: according to the proportion of adding 4kg of microbial agent to every 100kg of potato starch wastewater, firstly adding the microbial agent 3 into the potato starch wastewater, and then carrying out facultative fermentation on the wastewater added with the microbial agent for 120 days at the temperature higher than 4 ℃. Then respectively flushing the fermented wastewater into the wheat field in the jointing stage, the booting stage and the filling stage of the wheat, wherein the flushing amount of the wastewater is 60t/hm every time2。
Example four
The microbial preparation of the present example was prepared by mixing 50 parts of the complex microorganism and 50 parts of urea by weight. The composite microorganism is prepared by compounding aspergillus glaucus powder, mixed photosynthetic bacteria powder and bacillus amyloliquefaciens powder, wherein the mesh number of the three bacteria powder is 115 meshes of the aspergillus glaucus powder, 120 meshes of the mixed photosynthetic bacteria powder and 115 meshes of the bacillus amyloliquefaciens powder. The three bacterial powders are 15 parts by weight of aspergillus glaucus bacterial powder, 15 parts by weight of mixed photosynthetic bacterial powder and 17 parts by weight of bacillus amyloliquefaciens bacterial powder. The mixed photosynthetic bacteria powder is prepared by mixing rhodopseudomonas palustris powder, rhodospirillum rubrum powder and rhodobacter capsulatum powder according to the weight ratio of 1:1: 1.
The inventor prepares the three bacterial powders, on one hand, rhodopseudomonas palustris, rhodospirillum rubrum, rhodobacter capsulatum and bacillus amyloliquefaciens are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacterial bodies in the bacterial liquids are separated, concentrated and dried to obtain single bacterial strain powder. The bacterial content of each single bacterial strain powder is respectively as follows: the bacteria content of Rhodopseudomonas palustris is not less than 5.5 × 1010cfu/g, content of Rhodospirillum rubrum is greater than or equal to 4.51010cfu/g, the bacterium content of rhodobacter capsulatus is more than or equal to 5.0 multiplied by 1010cfu/g, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 3.5 multiplied by 1010cfu/g. On the other hand, the aspergillus glaucus is prepared into liquid seed liquid, then the liquid seed liquid is transferred into a corresponding solid culture medium, a solid culture is obtained by fermentation, then the solid culture is dried and crushed to prepare single-strain solid bacterial powder, wherein the bacterial content of the aspergillus glaucus is more than or equal to 4.0 multiplied by 109cfu/g。。
A microbial preparation 4 was prepared in the same manner as in example 1, except that the mixed photosynthetic bacteria powder was replaced with Rhodopseudomonas palustris powder.
The application method of the microbial preparation 4 comprises the following steps: according to the proportion of adding 5kg of microbial agent to every 100kg of potato starch wastewater, firstly adding the microbial agent 4 into the potato starch wastewater, and then carrying out facultative fermentation on the wastewater added with the microbial agent for 180 days at the temperature higher than 4 ℃. Then respectively flushing the fermented wastewater into the wheat field in the jointing stage, the booting stage and the filling stage of the wheat, wherein the flushing amount of the wastewater is 75t/hm every time2。
In order to help better understand the technical scheme of the invention, a test example of wheat planting is provided below for illustrating the application effect of the invention.
Test example: application effect of microbial preparation for treating potato starch wastewater on wheat planting
The field piece of the test is located in the Xinxiang city of Henan province, 5 groups of test designs comprise 4 test groups and 1 control group, each group of test designs 3 test cells, and the area of each test cell is 30m2All test cells are randomly distributed.
The test group applied the potato starch wastewater treated by the microbial preparation prepared by the invention. And respectively applying the treated wastewater into a wheat field in the jointing stage, the booting stage and the filling stage of the wheat. The four test groups are respectively applied with potato starch wastewater treated by microbial preparations 1-4, wherein the application amount of the potato starch wastewater treated by the microbial preparation 1 is 15t/hm every time2Through which is passedThe application amount of the potato starch wastewater treated by the microbial preparation 2 is 30t/hm every time2The application amount of the potato starch wastewater treated by the microbial preparation 3 is 60t/hm each time2The application amount of the potato starch wastewater treated by the microbial preparation 4 is 75t/hm every time2. The control group was not applied with the potato starch wastewater treated with the microbial preparation, and the rest of the operation was the same as that of the test group.
Selecting Xinmai No. 26 as a test variety, sowing in ten days of 10 months, wherein the row spacing is 25cm, and the sowing amount is 7.5kg/667m2. The same conventional management is adopted for each cell, and the cells are harvested in the 6 th last month of the next year. Before wheat harvest, 15 plants are selected from each cell for seed test, the plant height, the ear length, the number of formed ears, the number of grains per ear and the thousand grain weight of the wheat are counted, and the wheat yield of each cell is counted after the wheat is harvested. The average wheat plant height, average ear length, average ear number, average ear grain number, average thousand grain weight and average wheat yield of each cell were calculated. The results are shown in Table 1.
TABLE 1
As can be seen from the data in Table 1, the plant height, ear length, number of ears, number of grains per ear, thousand grain weight and wheat yield in the plot of the potato starch wastewater treated with the four groups of microbial agents were all significantly higher than the control. The potato starch wastewater treated by the microbial preparation can promote the growth of wheat and improve the yield of wheat.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.
Claims (10)
1. A microbial preparation for treating potato starch wastewater is prepared by mixing compound microorganisms and urea.
2. The microbial formulation of claim 1, wherein: the microbial preparation is prepared by mixing 40-50 parts of the compound microorganism and 50-60 parts of urea by weight.
3. The microbial preparation of claim 1 or 2, wherein: the microbial preparation is prepared by mixing 45-50 parts of the compound microbes and 50-55 parts of urea by weight.
4. The microbial preparation of claim 1 or 2, wherein: the microbial preparation is prepared by mixing 47 parts of compound microorganisms and 53 parts of urea by weight.
5. The microbial preparation of claim 1 or 2, wherein: the compound microorganism comprises aspergillus glaucus, photosynthetic bacteria and bacillus amyloliquefaciens.
6. The microbial formulation of claim 5, wherein: the photosynthetic bacteria are at least one of rhodopseudomonas palustris, rhodospirillum rubrum or rhodobacter capsulatum.
7. A method of use of a microbial preparation according to any one of claims 1 to 6, comprising the steps of:
step 1, adding a microbial preparation into potato starch wastewater according to a set proportion;
2, performing facultative fermentation on the wastewater treated in the step 1 at the temperature higher than 4 ℃;
and 3, flushing the wastewater treated in the step 2 into a wheat field.
8. The method of application according to claim 7, characterized in that: the weight ratio of the microbial agent to the potato starch wastewater in the step 1 is 1-5: 100.
9. The method of application according to claim 7, characterized in that: the fermentation time in the step 2 is 30-180 days.
10. The method of application according to claim 7, characterized in that: the flushing amount of the wastewater in the step 3 is 15-75t/hm every time2。
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