CN112781948B

CN112781948B - Cover glass absorbs and transfers mounting device and automatic mounting equipment

Info

Publication number: CN112781948B
Application number: CN202011521364.6A
Authority: CN
Inventors: 弥胜利; 杨健; 沈俊旭; 叶成
Original assignee: Guangdong Jinquan Medical Technology Co ltd
Current assignee: Guangdong Jinquan Medical Technology Co ltd
Priority date: 2020-12-21
Filing date: 2020-12-21
Publication date: 2023-01-31
Anticipated expiration: 2040-12-21
Also published as: CN112781948A

Abstract

The device comprises a suction head mechanism with a suction head and a reciprocating transfer mechanism, wherein the reciprocating transfer mechanism is coupled to the suction head mechanism to transfer the suction head mechanism between a cover glass suction station and a cover glass pressing station in a reciprocating manner, the suction head is triggered to open and suck to suck the cover glass or close and suck to release the cover glass on the corresponding station, the reciprocating transfer mechanism comprises a driving unit and an upright bearing plate, the driving unit is coupled to the suction head mechanism, the suction head mechanism is arranged on the upright bearing plate in a manner of moving along the surface of the upright bearing plate, the cover glass suction station and the cover glass pressing station are respectively arranged in a first area and a second area of the upright bearing plate, and a path for reciprocating transfer of the suction head mechanism is formed between the first area and the second area of the upright bearing plate. The device is favorable to realizing the automatic mounting operation of simple, high efficiency, high stability and accurate control, and the efficient simple operation of absorption and mounting processing is continuously guaranteed.

Description

Cover glass absorbs and transfers mounting device and automatic mounting equipment

Technical Field

The invention relates to automatic mounting processing equipment for biological tissue sample slices, in particular to a cover glass suction transfer mounting device and automatic mounting equipment.

Background

Pathological sections in biological tissue sample sections are widely applied, and generally pathological sections are prepared by taking pathological tissues with certain sizes and using a histopathology method, the pathological tissues are usually embedded in paraffin blocks, cut into slices by a slicer, stained by hematoxylin-eosin (H-E), further examined by a microscope for pathological changes, the occurrence and development processes of the pathological changes are observed, and finally pathological diagnosis is made. The automatic sealing processing technology of the biological tissue sample section is an important step in the whole automatic staining, drying and sealing process of the biological tissue sample, and is an indispensable step, so that the final biological tissue sample section has a convenient observed appearance and can be stored for a long time, and the sealing step is absolutely the core of the post-processing. Compare with the manual work and carry out the film-making, dye piece and mounting, automatic mounting requires to realize high efficiency and accurate control, requires to have high-efficient simple and easy method to carry out the mounting operation for a large amount of sections when can continuously guaranteeing to carry out the post processing.

Furthermore, automatic mounting also requires that the final sample be stored intact for about 20 years, which places high demands on the presence of impurities such as air bubbles in the coverslip. The traditional slide sealing machine carries out physical linear hard pressing when a cover glass is taken to seal a slide, so that bubbles are easily generated, the number of the bubbles in a sealed piece is relatively large, and subsequent observation and the storage quality and the storage time limit of the sealed piece are influenced.

The full automation of the traditional sealing machine is not complete, the full automation and the unmanned realization of the whole process of drying, dyeing, drying and sealing can not be realized, and a part of functions can be separated for independent treatment.

The conventional mounting machine is designed such that the slides are arranged in a rectangular array on the tray, and when the steps of dyeing, mounting and the like which require the operation of individual slides are performed, the slide module is basically fixed, and the cover glass module is moved and corrected in position.

It is noted that the information disclosed in the above background section is only for understanding of the background of the present application and therefore may include information that does not constitute prior art that is known to a person of ordinary skill in the art.

Disclosure of Invention

The present invention is to overcome the above problems in the prior art, and provides a cover glass sucking and transferring mounting device and an automatic mounting apparatus.

In order to realize the purpose, the invention adopts the following technical scheme:

a cover glass sucking and transferring sealing device comprises a sucker mechanism with a sucker and a reciprocating transfer mechanism, wherein the reciprocating transfer mechanism is coupled to the sucker mechanism so as to transfer the sucker mechanism between a cover glass sucking station and a sealing pressing station in a reciprocating mode, the sucker is triggered to open and suck to suck a cover glass or close and suck to release the cover glass on the corresponding station, the reciprocating transfer mechanism comprises a driving unit and an upright bearing plate, the driving unit is coupled to the sucker mechanism, the sucker mechanism is arranged on the upright bearing plate in a mode of moving along the surface of the upright bearing plate, the cover glass sucking station and the sealing pressing station are respectively arranged in a first area and a second area of the surface of the upright bearing plate, and the reciprocating path of the sucker transfer mechanism is formed between the first area and the second area of the upright bearing plate.

Further:

the drive unit is step motor, come and go transfer mechanism still including being located erect the loading board with rotation cantilever between the suction head mechanism, be provided with one section fretwork slide on the rotation cantilever, step motor passes through erect the through-hole on the loading board with the rotation cantilever is connected, it is provided with the gyro wheel slide that defines and come and go the transfer route to erect on the loading board, be fixed with the gyro wheel pole in the suction head mechanism, install on the gyro wheel pole and follow the rolling gyro wheel of gyro wheel slide, the gyro wheel pole passes fretwork slide on the rotation cantilever, step motor drives when rotating the cantilever by it drives to rotate the cantilever suction head mechanism along the gyro wheel slide removes, the fretwork slide does the degree of freedom that the gyro wheel pole provided is guaranteed rotate cantilever pivoted in-process suction head mechanism can follow-up.

The roller slideway is a round-corner inverted U-shaped slideway.

The reciprocating transfer mechanism also comprises a longitudinal moving guide rail, a transverse sliding block and a transverse moving guide rail, the sucker mechanism is slidably arranged on the longitudinal moving guide rail, the longitudinal moving guide rail is fixed on the transverse sliding block, the transverse sliding block is slidably arranged on the transverse moving guide rail, and the transverse moving guide rail is fixed on the vertical bearing plate, so that the sucker mechanism is always kept in a vertical state in the reciprocating transfer process; preferably, the suction head mechanism is arranged on the longitudinal moving guide rail through a slide block.

The suction head mechanism comprises a first suction head, a second suction head and a suction head platform, wherein the first suction head and the second suction head are fixed at different positions at the bottom of the suction head platform, when the suction head platform is in an intermediate state between a suction action and a sealing piece pressing action, the suction head platform keeps an inclined state to enable the first suction head to be lower than the second suction head, when the suction head platform is in an operation state of a downward suction action or a sealing piece pressing action, the first suction head is firstly jacked upwards to enable the suction head platform to gradually tend to be horizontal, and therefore the second suction head is enabled to be delayed to participate in the suction action or the sealing piece pressing action relative to the first suction head.

The suction head mechanism comprises a movable suction head bearing plate, a movable connecting rod, a longitudinal supporting plate, an upper limiting block, a lower limiting block and a trigger switch, wherein one end of the suction head platform is hinged to the lower end of the movable suction head bearing plate, the movable connecting rod is obliquely hinged between the other end of the suction head platform and the longitudinal supporting plate, the upper limiting block and the lower limiting block are fixed on the longitudinal supporting plate, the movable suction head bearing plate is slidably arranged on the longitudinal supporting plate between the upper limiting block and the lower limiting block, the reciprocating transfer mechanism is coupled to the longitudinal supporting plate, the trigger switch is arranged on the longitudinal supporting plate, so that when the movable suction head bearing plate reaches the upper limiting block, the suction head is triggered to be opened or closed, wherein when the suction head bearing plate is in an intermediate state between suction action and sealing plate pressing action, the movable suction head bearing plate is located on the lower limiting block under the action of gravity, and when the suction head is in an operating state of downward suction action or sealing plate pressing action, the suction head platform tends to be horizontal gradually by means of upward sliding of the movable suction head bearing plate and outward sliding of the movable connecting rod in the upward jacking process of the first suction head bearing plate.

The device is characterized in that the longitudinal supporting plate is provided with a sensing piece, the cover glass sucking station and the sealing piece pressing station are respectively provided with a sensor, and the sensors trigger the reciprocating transfer mechanism to return the longitudinal supporting plate to the opposite end station from the current station when sensing the sensing piece.

The longitudinal support plate is provided with a reciprocating limiting block, the cover glass sucking station and the sealing piece pressing station are respectively provided with a group of spring ejector pins and a stopping bolt, and the positions of the spring ejector pins are higher than the stopping bolts, so that the reciprocating limiting block firstly touches the spring ejector pins and then touches the stopping bolts on the stations, and the unloading force of the longitudinal support plate is slowly reduced.

The reciprocating limiting block is a beam structure which is vertically fixed at the upper end of the longitudinal supporting plate, and two ends of the beam structure are respectively provided with an induction sheet.

An automatic mounting device for biological tissue sample slices comprises the cover glass sucking and transferring mounting device.

The invention has the following beneficial effects:

the invention provides a cover glass sucking and transferring sealing device, wherein a sucker mechanism is arranged on a vertical bearing plate in a mode of moving along the surface of the vertical bearing plate, a cover glass sucking station and a pressing sealing station are respectively arranged in a first area and a second area of the surface of the vertical bearing plate, and a path for the sucker mechanism to be transferred back and forth by a back and forth transfer mechanism is formed between the first area and the second area of the vertical bearing plate, so that the cover glass sucking and transferring sealing device is beneficial to realizing simple, high-efficiency, high-stability and accurately-controlled automatic sealing operation, and continuously ensures efficient and simple operation of sucking and sealing treatment.

The cover glass suction and transfer mounting device has high efficiency, simplicity, accuracy and high repeatability in cover glass transfer operation, and is suitable for fully automatically completing cover glass suction and mounting operation. The invention can efficiently and accurately carry out fixed path transportation on a single cover glass, and achieves the effect of batch processing of glass slides in a simple repeated operation mode.

In a preferred scheme, the cover glass sucking and transferring sealing device disclosed by the invention not only can realize full automation of cover glass sucking and cover glass sealing operations and obtain high precision, but also can avoid the problem that bubbles are easily generated under a cover glass in the sealing process, obviously improves the sealing quality, and is beneficial to subsequent observation and extension of the storage time limit of the sealing.

Since the first suction head and the second suction head are fixed at different positions on the bottom of the suction head platform, the reciprocating transfer mechanism is coupled to the suction head platform and is arranged in such a way that when the suction head platform is in an intermediate state between the suction action and the sealing piece pressing action, the suction head platform keeps an inclined state so that the first suction head is lower than the second suction head, and when the suction head platform is in an operation state of downward suction action or sealing piece pressing action, the first suction head is firstly jacked up so that the suction head platform gradually tends to be horizontal, thereby enabling the second suction head to be delayed to participate in the suction action or the sealing piece pressing action relative to the first suction head. Therefore, the cover glass sucking and transferring sealing device has a sealing action mode different from the traditional physical straight line hard pressing mode, and forms a side pressure progressive sealing mode starting from one side.

The invention provides a slicing and mounting device for batch processing of the whole mounting process, which can achieve the effect of batch processing of glass slides in the simplest operation mode of a system.

The invention can achieve the effects of automatic batch mounting treatment of biological tissue samples in the preorder dyeing process, multi-degree-of-freedom movement of the cover glass, automatic monitoring of the allowance and position of the cross-linking agent of the cover glass, automatic titration of the cross-linking agent, removal of redundant cross-linking agent, consumption saving of the cross-linking agent, high efficiency, time saving and prevention of a large amount of bubbles generated under the cover glass in the mounting process.

The invention provides a batch, efficient, simple and accurate full-automatic mounting scheme, which can automatically drop a cross-linking agent and reduce bubbles generated in the mounting process as much as possible.

Drawings

FIG. 1 is a schematic view showing the overall configuration of a cover glass sucking and transferring mounting device according to an embodiment of the present invention.

FIG. 2 is a schematic diagram showing the detailed structure of the suction head of the cover glass sucking and transferring device according to one embodiment of the present invention.

FIG. 3 is a schematic view showing the final state of the cover glass sucking and transferring device when the tip part of the cover glass sucking and transferring device is forced to suck the cover glass and press the cover glass according to one embodiment of the present invention.

Fig. 4 is a schematic structural view of the cover glass sucking and transferring mounting device of one embodiment of the present invention after hiding the longitudinal support plate and the limiting block.

Fig. 5 is a schematic structural view of the cover glass sucking and transferring mounting device in operation according to an embodiment of the present invention.

Fig. 6 is a schematic view showing the final state of the cover slip sucking and transferring slide device in one cover slip-mounting process according to the embodiment of the present invention.

Fig. 7 is a partially enlarged view showing the final state of the cover glass sucking and transferring mounting device in one cycle according to the embodiment of the present invention.

FIG. 8 is an overall schematic view of a crosslinker titration apparatus in accordance with an embodiment of the present invention.

FIG. 9 is a schematic view of a major portion of a crosslinker titration apparatus of one embodiment of the present invention with minor components hidden.

FIG. 10 is an enlarged schematic view of a main part in the operation of a crosslinking agent titration apparatus according to an embodiment of the present invention.

FIG. 11 is a schematic view of an end-of-cycle condition of a crosslinker titration apparatus in accordance with an embodiment of the present invention.

Figure 12 is a schematic view of the overall configuration of the slide tray transfer device and the coverslip loading transport device of one embodiment of the present invention.

Figure 13 is a bottom perspective view of a slide tray transfer device according to one embodiment of the present invention.

Fig. 14 is a schematic structural view of a cover glass loading and transporting apparatus according to an embodiment of the present invention.

Figure 15 is one of the detailed views of the coverslip loading transport apparatus of one embodiment of the present invention.

Figure 16 is a second detail view of the coverslip loading transport apparatus of one embodiment of the present invention.

Fig. 17 is a schematic view of a cover glass loading process of the cover glass loading and transporting apparatus according to an embodiment of the present invention.

FIG. 18 is a general schematic view of the back side of the automated coverslipping apparatus for sections of biological tissue samples in accordance with one embodiment of the present invention.

FIG. 19 is a schematic overall view of the front side of an automated mounting apparatus for biological tissue sample slices according to one embodiment of the present invention.

Reference numerals:

1. a stepping motor; 2. erecting a bearing plate; 3. EE photoelectric limit switch; 4. a spring thimble; 5. a stop bolt; 6. a lateral movement guide rail; 7. a longitudinally moving guide rail; 8. a transverse slide block; 9. a fixed block; 10. a slide block for moving the suction head bearing plate; 11. moving the suction head bearing plate; 12. a suction head; 13. a suction head platform; 14. a movable connecting rod; 15. a touch switch; 16. a longitudinal support plate; 17. a lower limiting block; 18. an upper limit block; 19. a reciprocating limiting block; 20. rotating the cantilever; 21. a roller slideway; 22. moving the suction head bearing plate guide rail; 23. an air pump coupling head; 24. a roller; 25. a slider for supporting the plate longitudinally; 26. an induction sheet; 27. a vertical fixing plate with a window; 28. a second support plate; 29. a peristaltic pump; 30. a spray head; 31. a second synchronizing wheel; 32. an induction sheet; 33. a rotating arm; 34. a crosslinker reagent bottle; 35. a cross-linked reagent bottle cartridge; 36. soaking the tank; 37. an inductor; 38. a first synchronization wheel; 39. 40, a limiting block of the rotating arm; 41. a slide tray; 42. a glass slide; 43. a positioning pin of the slide tray; 44. a first support plate in a T shape; 45. covering the glass box; 46. the cover glass box bears the moving holder; 47. an indexing dial; 48. a photodetector on the index dial; 49. an I/O port; 50. an elastic ball; 51. a cradle head moving guide rail; 52. a cradle head sliding block; 53. a side baffle limiting block; 54. a ball screw hole; 55. a side dam; 56. a top package board; 57. and (6) fixing the rod.

Detailed Description

The embodiments of the present invention will be described in detail below. It should be emphasized that the following description is merely exemplary in nature and is not intended to limit the scope of the invention or its application.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element. In addition, the connection can be for fixation or for coupling or communication.

It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the embodiments of the present invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed in a particular orientation, and be in any way limiting of the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the embodiments of the present invention, "a plurality" means two or more unless specifically limited otherwise.

Referring to fig. 1, 4 to 6, in some embodiments, a coverslipping transfer device includes a tip mechanism having a tip (e.g., a first tip 12) coupled to the tip mechanism to transfer the tip mechanism to and from a coverslipping station and a coverslipping station, the tip being triggered to open suction to aspirate a coverslip or close suction to release a coverslip at the corresponding station, and a shuttle mechanism including a driving unit coupled to the tip mechanism, the tip mechanism being disposed on the upright carrier plate 2 so as to be movable along a surface of the upright carrier plate 2, and the coverslipping station being disposed at a first region and a second region of the surface of the upright carrier plate 2, respectively, a path of the shuttle of the tip mechanism being formed between the first region and the second region of the upright carrier plate 2.

As shown in fig. 1 and fig. 4 to fig. 7, in a preferred embodiment, the driving unit is a stepping motor 1, the reciprocating transfer mechanism further includes a rotating cantilever 20 located between the vertical bearing plate 2 and the suction head mechanism, the rotating cantilever 20 is provided with a section of hollow slideway, the stepping motor 1 passes through a through hole on the vertical bearing plate 2 and is connected with the rotating cantilever 20, a roller slideway 21 defining a reciprocating transfer path is provided on the vertical bearing plate 2, a roller rod is fixed on the suction head mechanism, a roller 24 along the roller slideway 21 is installed on the roller rod, the roller rod passes through the hollow slideway on the rotating cantilever 20, the stepping motor 1 drives the rotating cantilever 20 while the rotating cantilever 20 is driven by the rotating cantilever 20 to drive the suction head mechanism along the roller slideway 21 to move, the hollow slideway is the degree of freedom provided by the roller rod to ensure that the rotation cantilever 20 can follow the suction head mechanism in the rotating process. In a preferred embodiment, the roller ramps 21 are rounded U-shaped ramps.

As shown in fig. 1, 4 to 7, in a preferred embodiment, the shuttle mechanism further comprises a longitudinal moving guide 7, a transverse slide 8 and a transverse moving guide 6, the suction head mechanism is slidably disposed on the longitudinal moving guide 7, the longitudinal moving guide 7 is fixed on the transverse slide 8, the transverse slide 8 is slidably disposed on the transverse moving guide 6, and the transverse moving guide 6 is fixed on the vertical loading plate 2, so that the suction head mechanism is always kept in a vertical state during the shuttle. Preferably, the suction head mechanism can be arranged on the longitudinal moving guide 7 by means of a slide.

Referring to fig. 1 to 3, in a preferred embodiment, the nozzle mechanism includes a first nozzle 12, a second nozzle 12 and a nozzle platform 13, the first nozzle 12 and the second nozzle are fixed at different positions on the bottom of the nozzle platform 13, wherein, in an intermediate state between the sucking action and the cover pressing action, the nozzle platform 13 is kept in a tilted state so that the first nozzle 12 is positioned lower than the second nozzle, and in an operating state of the downward sucking action or the cover pressing action, the first nozzle 12 is first lifted up to gradually level the nozzle platform 13, thereby causing the second nozzle to be delayed from participating in the sucking action or the cover pressing action with respect to the first nozzle 12.

As shown in fig. 1 to 6, in a preferred embodiment, the suction head mechanism includes a movable suction head carrier plate 11, a movable link 14, a trigger switch 15, a longitudinal support plate 16, a lower limit block 17 and an upper limit block 18, one end of the suction head platform 13 is hinged to a lower end of the movable suction head carrier plate 11, the movable link 14 is obliquely hinged between the other end of the suction head platform 13 and the longitudinal support plate 16, the upper limit block 18 and the lower limit block 17 are fixed to the longitudinal support plate 16, the movable suction head carrier plate 11 is slidably disposed on the longitudinal support plate 16 between the upper limit block 18 and the lower limit block 17, the shuttle mechanism is coupled to the longitudinal support plate 16 to shuttle the longitudinal support plate 16 between a cover glass suction station and a cover sheet pressing station, the trigger switch 15 is disposed on the longitudinal support plate 16 to trigger the open suction or the close suction when the movable carrier plate 11 reaches the upper limit block 18, wherein, when in an intermediate state between the suction action and the cover sheet pressing action, the movable suction head carrier plate 11 is seated on the lower limit block under the action of gravity, the movable suction head carrier plate 11 is gradually moved upward and the movable suction head carrier plate 13 is moved toward a horizontal state by the first suction head carrier plate 14.

In a preferred embodiment, as shown in fig. 1, 4 to 6, the moving tip carrier plate 11 is arranged on the longitudinal support plate 16 by means of a slide 10. The longitudinal support plate 16 can be arranged on the longitudinal movement rail 7 by means of a slide 25.

As shown in fig. 1, 5 to 7, in a preferred embodiment, a sensing piece 26 is disposed on the longitudinal support plate 16, and a sensor, such as an EE photoelectric limit switch 3, is disposed at each of the cover glass suction station and the cover glass pressing station, and when the sensing piece 26 is sensed, the sensor triggers the shuttle mechanism to return the longitudinal support plate 16 from the current station to the opposite station.

As shown in fig. 1 and fig. 5 to 7, in a preferred embodiment, a reciprocating limiting block 19 is disposed on the longitudinal supporting plate 16, a set of pogo pins 4 and a stopping bolt 5 are respectively disposed at the cover glass sucking station and the sealing sheet pressing station, and the pogo pins 4 are higher than the stopping bolt 5, so that the reciprocating limiting block 19 contacts the pogo pins 4 and then contacts the stopping bolt 5 at the station, so that the unloading force of the longitudinal supporting plate 16 is slowly reduced.

In a preferred embodiment, the reciprocating limiting block 19 is a beam structure vertically fixed on the upper end of the longitudinal support plate 16, and two ends of the beam structure are respectively provided with a sensing piece 26.

In other embodiments, an automatic mounting device for biological tissue sample slices comprises the cover glass suction and transfer mounting device of any one of the previous embodiments.

The following further illustrates the automatic mounting device for biological tissue sample slices and the cover glass sucking and transferring mounting device provided therein according to the embodiment of the present invention.

As shown in fig. 1 to 19, in one embodiment, an automatic mounting apparatus for a biological tissue sample slice mainly comprises: the device comprises a slide glass tray conveying device, a cross-linking agent titration device, a cover glass loading and conveying device and a cover glass sucking and transferring sealing device.

As shown in fig. 1 to 7, in the cover glass suction and mounting device of some embodiments, the upright carrier plate 2 is fixedly connected with the stepping motor 1, the longitudinal support plate 16, the lateral movement guide rail, the EE photoelectric limit switch 3 and other components mounted thereon by screws, and the upright carrier plate 2 plays an integral fixed supporting role and provides a planar movement path. The stepping motor 1 powers the reciprocating movement of the longitudinal support plate 16, and the stepping motor 1 can reverse the characteristics of the rotation direction to achieve the repeatability of the reciprocating movement of the longitudinal support plate 16.

The stepping motor 1 can be in interference fit with the shaft hole of the rotating cantilever 20 through the circular hole on the vertical bearing plate 2. The rotating cantilever 20 is provided with a section of hollow slide way, the hollow slide way provides more degrees of freedom for the rollers on the longitudinal support plate 16, and the longitudinal support plate 16 can be ensured to follow up in the rotating process of the rotating cantilever 20.

A roller 24 is fixed to the upper end of the longitudinal support plate 16 by a screw, and the roller 24 is caught in the roller slide 21 of the upright support plate 2. The upper end of the longitudinal support plate 16 is also fixed with a reciprocating limiting block 19 through a screw. The lower end of the longitudinal support plate 16 fixes the trigger switch 15, the longitudinal moving guide rail 7, the movable connecting rod 14 and other components through screws.

The reciprocating stopper 19 is used to limit the limit position of the movement of the longitudinal support plate 16. The vertical bearing plate 2 is provided with a spring thimble 4 and a stop bolt 5 corresponding to the limit position. Before the longitudinal support plate 16 moves to the limit positions of the two stations, the reciprocating limiting block 19 firstly touches the spring ejector pin 4 and then touches the stop bolt 5, so that the longitudinal support plate 16 and the suction head carried by the longitudinal support plate cannot cross the limit positions.

The height of the spring ejector pin 4 is a little higher than that of the stop bolt, so that the limiting block firstly touches the spring ejector pin 4 in the moving process to provide the function of unloading in advance. In addition, the height of the stop bolt can be adjusted, and the height of the limiting position of the sucker can also be adjusted, so that more flexibility is brought to the device.

Two induction sheets 26 are fixed on two sides of the reciprocating limiting block 19 by screws, and the corresponding induction sheets 26 can trigger the corresponding EE photoelectric switch 3 at two limiting positions and then control the switch of the stepping motor.

The transverse moving guide rail 6 is fixedly connected with the vertical bearing plate 2 through a screw. The lateral movement guide 6 can support the lateral slider 8 sliding thereon. The transverse slide block 8 is connected with the longitudinal moving guide rail 7 by a screw and a fixed block 9. The longitudinal support plate 16 is movable in a vertical direction perpendicular to the lateral movement rail 6 by the longitudinal movement rail 7, and is movable in a horizontal direction along the lateral movement rail 6 by the lateral slider 8.

The trigger switch 15 is fixed on the longitudinal support plate 16, when the lower moving suction head bearing plate 11 touches the trigger switch 15 during upward movement, the trigger switch 15 generates a trigger signal, and the air pump is controlled to be turned on or off according to the station where the longitudinal support plate 16 is located, so that the suction head can open or close suction.

The middle part of the moving tip carrier plate 11 is fixed by means of screws and a slide 10, is slidably arranged on the longitudinal support plate 16 by means of the slide 10, and the extreme sliding position of the moving tip carrier plate 11 is defined by means of stop blocks 17, 18 fixed to the longitudinal support plate 16. The lower end of the mobile suction head bearing plate 11 is connected with the suction head platform 13 by bolts, and the joint can rotate. The lower end of the longitudinal support plate 16 is connected with the suction head platform 13 through a movable connecting rod 14, and the movable connecting rod 14 keeps inclining. By this connection it is ensured that in normal conditions the first suction head 12 and the second suction head are not in the same horizontal line, the first suction head 12 being positioned lower than the second suction head, and that the suction head platform 13 can be gradually brought to level by the outward turning of the movable link 14 and the upward sliding of the moving suction head carrier plate 11 when the first suction head 12 is lifted.

Referring to fig. 1 to 3, in the preferred embodiment, for the initial state of the cover glass sucking and transferring device, the suction head 12 in the low position contacts the cover glass first, then the suction head platform 13 gradually tends to be horizontal along with the slow downward movement of the longitudinal support plate 16, in the process, the movable connecting rod 14 rotates, the suction head platform 13 pushes the movable suction head bearing plate 11 to move upwards, the movable suction head bearing plate 11 moves upwards together with the slide block 10 fixed together with the movable suction head bearing plate until the upper limit block 18 is touched and the touch switch 15 is triggered, then the air pump is started, and the suction head sucks the cover glass.

Referring to fig. 1, 4 to 6, in a preferred embodiment, after the air pump is started and the two suction heads suck the cover glass, the sensing piece 26 on the limiting block triggers the EE photoelectric switch 3, the stepping motor 1 is started, the rotation of the rotating cantilever 20 makes the longitudinal support plate 16 and the roller 24 thereon move on a plane along the slide track 21 on the upright carrying plate 2, meanwhile, the roller rod also moves in the slide on the rotating cantilever 20, the longitudinal support plate 16 follows along the longitudinal moving guide rail 7, and the transverse slide 8 also follows along the longitudinal moving guide rail 7, the longitudinal support plate 16 and the slide 25 thereof on the transverse moving guide rail 6.

Referring to fig. 5, in the preferred embodiment, during the operation of the stepping motor 1, the cover glass is separated from the original position, and under the action of gravity, the suction head carrier plate 11 is moved vertically downwards and clamped on the lower limiting block 17, so that the space posture of the cover glass is still not horizontal during transportation, and the whole longitudinal carrier plate is always kept vertical under the action of a plurality of sliding rails.

Referring to fig. 7, in the preferred embodiment, when the structure is operated to the second half, the stop block 19 contacts the spring thimble 4 first, then contacts the stop bolt 5, and after the force releasing process, the sensor sheet 26 beside the stop block 19 slowly descends to trigger the EE photoelectric switch 3, so that the stepping motor 1 stops rotating and reverses after several seconds.

Referring to fig. 6 to 7, in a preferred embodiment, the lower position of the double suction head 12 carrying the cover glass first contacts the slide glass, i.e. the lower end first covers the cover glass, during the process of slowly descending the longitudinal support plate 16, the suction head platform 13 gradually levels until the cover glass at the upper end also firmly contacts the slide glass, during this process, the suction head bearing plate 11 together with the slide block 10 slides up to the upper limit block 18, which is equivalent to pushing out air bubbles from the single-side cover glass by force to complete the sealing, and triggering the trigger switch 15, at this time, the air pump is turned off, the stepping motor 1 reverses to the initial position, a step of completely sucking the single cover glass and obliquely pressing the sealing is completed, and the batch processing can be completed by continuously repeating the above processes.

The background of the present invention may contain background information related to the problem or environment of the present invention and does not necessarily describe the prior art. Accordingly, the inclusion in the background section is not an admission of prior art by the applicant.

The foregoing is a more detailed description of the invention in connection with specific/preferred embodiments and is not intended to limit the practice of the invention to those descriptions. It will be apparent to those skilled in the art that numerous alterations and modifications can be made to the described embodiments without departing from the inventive concepts herein, and such alterations and modifications are to be considered as within the scope of the invention. In the description of the present specification, reference to the description of "one embodiment," "some embodiments," "preferred embodiments," "example," "specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Those skilled in the art will be able to combine and combine features of different embodiments or examples and features of different embodiments or examples described in this specification without contradiction. Although embodiments of the present invention and their advantages have been described in detail, it should be understood that various changes, substitutions and alterations can be made herein without departing from the scope of the claims.

Claims

1. A cover glass sucking and transferring sealing device is characterized by comprising a sucker mechanism with a sucker and a reciprocating transferring mechanism, wherein the reciprocating transferring mechanism is coupled to the sucker mechanism to transfer the sucker mechanism between a cover glass sucking station and a sealing sheet pressing station in a reciprocating manner, the sucker is triggered to open and suck to suck a cover glass or close and suck to release the cover glass at a corresponding station, and the sucker is enabled to open and suck or close to suck by controlling the opening or closing of an air pump; the reciprocating transfer mechanism comprises a driving unit and an upright bearing plate, the driving unit is coupled to the sucker mechanism, the sucker mechanism is arranged on the upright bearing plate in a movable mode along the surface of the upright bearing plate, the cover glass sucking station and the sealing piece pressing station are respectively arranged in a first area and a second area of the surface of the upright bearing plate, and a reciprocating transfer path of the sucker mechanism is formed between the first area and the second area of the upright bearing plate; the sucker mechanism comprises a first sucker, a second sucker, a sucker platform, a movable sucker bearing plate, a movable connecting rod, a longitudinal supporting plate, an upper limiting block, a lower limiting block and a touch switch, the first suction head and the second suction head are fixed at different positions at the bottom of the suction head platform, wherein the first suction head and the second suction head are respectively positioned at two ends of the bottom surface of the suction head platform, one end of the top of the sucker platform is hinged with the lower end of the movable sucker bearing plate, the movable connecting rod is obliquely hinged between the other end of the top of the sucker platform and the longitudinal supporting plate, the upper limiting block and the lower limiting block are fixed on the longitudinal support plate, the movable sucker bearing plate is slidably arranged between the upper limiting block and the lower limiting block on the longitudinal support plate, the shuttle mechanism is coupled to the longitudinal support plate, the tact switch is disposed on the longitudinal support plate, so as to trigger the suction head to open or close suction when the movable suction head bearing plate reaches the upper limiting block, wherein, when the suction head is in an intermediate state between the sucking action and the sealing sheet pressing action, the movable suction head bearing plate is located on the lower limiting block under the action of gravity, the tip platform is maintained in an inclined state such that the first tip is positioned lower than the second tip, when the first suction head is in the operation state of the downward suction action or the sealing sheet pressing action, the first suction head is firstly jacked up, the entire bottom surface of the tip platform gradually tends to level during upward jacking of the first tip by means of the upward sliding movement of the moving tip carrier plate and the outward rotation of the movable link, thereby delaying the participation of the second suction head in the sucking action or the flap pressing action with respect to the first suction head.

2. The cover glass absorbing and transferring sealing device according to claim 1, wherein the driving unit is a stepping motor, the reciprocating transfer mechanism further comprises a rotating cantilever located between the vertical bearing plate and the suction head mechanism, the rotating cantilever is provided with a section of hollowed-out slide way, the stepping motor is connected with the rotating cantilever through a through hole on the vertical bearing plate, the vertical bearing plate is provided with a roller slide way defining a reciprocating transfer path, the suction head mechanism is fixed with a roller rod, the roller rod is provided with a roller wheel rolling along the roller slide way, the roller rod passes through the hollowed-out slide way on the rotating cantilever, the stepping motor drives the rotating cantilever and simultaneously drives the suction head mechanism to move along the roller slide way, and the hollowed-out slide way ensures that the suction head mechanism can follow the rotating cantilever in the rotating process.

3. The cover glass suction transfer coverslipping device of claim 2, wherein said roller slides are rounded-off U-shaped slides.

4. The cover glass sucking and transferring mounting device according to any one of claims 1 to 3, wherein the shuttle mechanism further comprises a longitudinal moving guide rail on which the tip mechanism is slidably disposed, a lateral slide rail on which the longitudinal moving guide rail is fixed, and a lateral moving guide rail on which the lateral slide rail is slidably disposed, the lateral moving guide rail being fixed on the upright carrying plate, thereby allowing the tip mechanism to be kept in an upright state at all times during the shuttle; the sucker mechanism is arranged on the longitudinal moving guide rail through a sliding block.

5. The cover glass sucking and transferring sealing device as claimed in any one of claims 1 to 3, wherein a reciprocating limiting block is arranged on the longitudinal supporting plate, the reciprocating limiting block is a beam structure which is vertically fixed on the upper end of the longitudinal supporting plate, two ends of the beam structure are respectively provided with a sensing piece, the cover glass sucking station and the sealing piece pressing station are respectively provided with a sensor, and the sensor triggers the reciprocating transferring mechanism to transfer the longitudinal supporting plate from the current station to the opposite station when sensing the sensing piece.

6. The cover glass sucking and transferring sealing device as claimed in claim 5, wherein the cover glass sucking station and the sealing pressing station are respectively provided with a set of spring ejector pins and stop bolts, and the positions of the spring ejector pins are higher than the stop bolts, so that the reciprocating limiting block firstly touches the spring ejector pins and then touches the stop bolts on the station, thereby the unloading force of the longitudinal supporting plate is slowly reduced.

7. An automatic mounting apparatus for a biological tissue sample section, comprising the cover glass suction transfer mounting device according to any one of claims 1 to 6.